CN108142443B - Asarum sieboldii extract and application thereof as plant antibacterial - Google Patents
Asarum sieboldii extract and application thereof as plant antibacterial Download PDFInfo
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- 239000000284 extract Substances 0.000 title claims abstract description 63
- 241000196324 Embryophyta Species 0.000 title claims abstract description 16
- 230000000844 anti-bacterial effect Effects 0.000 title abstract description 10
- 241001289295 Asarum sieboldii Species 0.000 title description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 53
- 241000758794 Asarum Species 0.000 claims abstract description 32
- 239000006286 aqueous extract Substances 0.000 claims abstract description 11
- 238000004519 manufacturing process Methods 0.000 claims abstract description 11
- 238000001914 filtration Methods 0.000 claims abstract description 8
- 238000005303 weighing Methods 0.000 claims abstract description 8
- 241000125121 Aspergillus carbonarius Species 0.000 claims abstract description 7
- 239000000706 filtrate Substances 0.000 claims abstract description 7
- 238000002791 soaking Methods 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 238000001035 drying Methods 0.000 claims abstract description 5
- 239000000843 powder Substances 0.000 claims abstract description 5
- 238000000605 extraction Methods 0.000 claims abstract description 4
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 4
- 230000005764 inhibitory process Effects 0.000 claims description 34
- 241000123650 Botrytis cinerea Species 0.000 claims description 23
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 18
- 239000006013 carbendazim Substances 0.000 claims description 18
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 18
- 241000219095 Vitis Species 0.000 claims description 16
- 230000001580 bacterial effect Effects 0.000 claims description 15
- 241000222199 Colletotrichum Species 0.000 claims description 13
- 230000002401 inhibitory effect Effects 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 9
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- 239000003814 drug Substances 0.000 claims description 6
- 201000002909 Aspergillosis Diseases 0.000 claims description 5
- 208000036641 Aspergillus infections Diseases 0.000 claims description 5
- 239000006229 carbon black Substances 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 241000228245 Aspergillus niger Species 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 235000009392 Vitis Nutrition 0.000 claims description 2
- 239000012675 alcoholic extract Substances 0.000 claims description 2
- 230000002223 anti-pathogen Effects 0.000 claims 1
- 235000013305 food Nutrition 0.000 abstract description 4
- 240000000560 Citrus x paradisi Species 0.000 abstract description 3
- 241001465754 Metazoa Species 0.000 abstract description 2
- 230000000853 biopesticidal effect Effects 0.000 abstract description 2
- 231100000956 nontoxicity Toxicity 0.000 abstract description 2
- 238000001228 spectrum Methods 0.000 abstract description 2
- 235000009754 Vitis X bourquina Nutrition 0.000 description 12
- 235000012333 Vitis X labruscana Nutrition 0.000 description 12
- 235000014787 Vitis vinifera Nutrition 0.000 description 12
- 241000219094 Vitaceae Species 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 235000021021 grapes Nutrition 0.000 description 7
- 239000000575 pesticide Substances 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000003053 toxin Substances 0.000 description 6
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- 241000193738 Bacillus anthracis Species 0.000 description 5
- 230000003385 bacteriostatic effect Effects 0.000 description 5
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- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000758795 Aristolochiaceae Species 0.000 description 2
- 241001529387 Colletotrichum gloeosporioides Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
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- 229920001817 Agar Polymers 0.000 description 1
- 241001465180 Botrytis Species 0.000 description 1
- 241000688200 Cingulata Species 0.000 description 1
- 241000326334 Coniella diplodiella Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000061457 Solanum nigrum Species 0.000 description 1
- 235000002594 Solanum nigrum Nutrition 0.000 description 1
- 101710182532 Toxin a Proteins 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- HQVFCQRVQFYGRJ-UHFFFAOYSA-N formic acid;hydrate Chemical compound O.OC=O HQVFCQRVQFYGRJ-UHFFFAOYSA-N 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 150000002611 lead compounds Chemical class 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to an asarum extract, which is an aqueous extract or an alcohol extract, wherein the alcohol extract extraction method comprises the following steps: weighing 800g of a crushed sample, soaking plant dry powder in 5 times of ethanol, and standing at room temperature for 12 h; ultrasonic extraction is carried out for 2 h; vacuum filtering, continuously extracting for 3 times, mixing filtrates, concentrating at 45 deg.C with rotary evaporator, drying, and storing at 4 deg.C; also includes the application of the asarum extract as a plant antibacterial, wherein the asarum extract can obviously inhibit the production of aspergillus carbonarius ochraceus A (OTA). The asarum extract used in the invention is a botanical biopesticide, has the characteristics of high antibacterial activity, wide antibacterial spectrum, difficult generation of resistance and no toxicity to human and animals, and can improve the food safety of grape fruits.
Description
Technical Field
The invention relates to the field of biological pesticides, in particular to a botanical biological pesticide, and more particularly relates to an asarum extract and application thereof as a plant antibacterial.
Background
Grapes are one of the oldest, most widely distributed fruits in the world. The cultivation history is long-flowing, and the yield accounts for about one fourth of the total fruit yield in the world. More than 70 species of Vitis have been reported globally, more than 8000 species of Vitis species, distributed in temperate and subtropical regions, generally 20-52 ° north latitude and 30-45 ° south latitude. According to the difference of geographical origin, the grapes are divided into three groups of north america, europe asia and east asia. China is one of the most abundant countries in the distribution of the grape plant resources, and the east Asia population has 42 known varieties of China, 1 variety of subspecies and 12 varieties. The grape variety is planted by about 500 varieties, and the grape planting area is second in the world and is second only to Spain. In 2016, the area of grapes in China exceeds 800 kilo hectares (1240 thousand mu), and the yield of fresh grapes is stable in the first place in the world since 2000 years.
The grapes are good in appearance and flavor and rich in nutrition, and good economic benefits and social benefits are obtained in grape cultivation in nearly 20 years, so that the grape industry in China develops rapidly. However, like other horticultural crops, the serious damage of diseases and pests of grapes is a limiting factor for the healthy development of industry, and common grape diseases such as grape gray mold, white rot, anthracnose and the like which are serious in damage; the damage of the diseases seriously affects the quality and the yield of the grapes, and is not only a difficulty which needs to be solved in the grape production in China, but also a difficult problem in the grape production worldwide. In addition, mycotoxins are important factors affecting food safety, among which the most important are the toxin a (ota) produced by aspergillus carbonarius; the toxin with excessive content can cause great harm to human body and even cause cancer. In agricultural production, chemical pesticides are used as a common prevention and control method, but chemical prevention and control have the risks of causing drug resistance, residual pollution, rampant and the like of grape pathogenic bacteria. Therefore, the search for non-chemical pesticides is an important task for the development of industry and an important subject of scientific research. The method for mining plant sources from plants, including developing plant source pesticides, searching lead compounds from plant source compounds and even directly utilizing plant leaching liquor, is an important content in the development direction.
Asarum (Asarum sieboldii Miq.) is a plant of Asarum (Aristolochiaceae) in Aristolochiaceae, and is produced in Shandong, Anhui, Zhejiang, Jiangxi, Henan, Hubei, Shaanxi and Sichuan in the soil for planting in the forest with the elevation of 1200-year-old 2100 m in the shade and wet rot. Also in Japan and Korea. The whole herb is used as medicine. But reports and researches on applying the compound as a biopesticide to control grape diseases are not found.
Disclosure of Invention
The invention mainly solves the technical problem of providing the asarum extract and the application thereof as a plant antibacterial, can achieve ideal prevention and treatment effects, has a remarkable inhibition effect on OTA production capacity of aspergillus carbonarius, and can improve the safety of grape food.
In order to solve the technical problems, the invention adopts the technical scheme that:
the asarum extract is an aqueous extract or an alcohol extract, and the aqueous extract extraction method comprises the following steps: weighing herba asari, pulverizing into dry 100g, adding 1000mL distilled water, and soaking for 30 min. Boiling with strong fire, decocting with slow fire for 40min, filtering the medicinal liquid with 4 layers of gauze, repeatedly extracting for one time, mixing filtrates, concentrating with slow fire to 1000mg/mL, and storing at 4 deg.C; the method for extracting the alcohol extract comprises the following steps: weighing 800g of a crushed sample, soaking plant dry powder in 5 times of ethanol, and standing at room temperature for 12 h; ultrasonic extraction is carried out for 2 h; vacuum filtering, continuously extracting for 3 times, mixing filtrates, concentrating at 45 deg.C with rotary evaporator, drying, and storing at 4 deg.C.
The asarum extract has obvious bacteriostatic activity on pathogenic bacteria of botrytis cinerea, botrytis cinerea and aspergillus niger.
Further, the colletotrichum gloeosporioides comprises a highly-resistant colletotrichum gloeosporioides strain to carbendazim, and the botrytis cinerea comprises a highly-resistant botrytis cinerea strain to carbendazim.
Furthermore, the concentration of the water extract is 6.25mg/mL-100mg/mL, and the concentration of the alcohol extract is 0.3125mg/mL-5 mg/mL.
Further, when the water extract is 100mg/mL, the bacteriostasis rates of the water extract on anthrax, white rot and gray mold are 40.40%, 74.87% and 37.50%; when the concentration of the alcohol extract is 5mg/mL, the inhibition rate of the alcohol extract on botrytis cinerea is 97.13%, and the inhibition rate on white rot, colletotrichum and carbon black aspergillosis is up to 100%. When the concentration of the alcohol extract is 2.5mg/mL, the bacteriostasis rate to 4 pathogenic bacteria is over 85.5 percent; when the concentration of the alcohol extract is 5mg/mL, the inhibition rates of the alcohol extract on multi-bacterium sensitive colletotrichum anthracnose bacterial strains FJZZ-62, coll-9 and coll-20 are respectively 96.30%, 94.33% and 96.88%, the inhibition rates on carbendazim high-resistant colletotrichum anthracnose bacterial strains FJND-9 and GZSD-89 are respectively up to 100%, the inhibition rate on FJND-40 is also up to 96.09%, the inhibition rates on multi-bacterium sensitive botrytis cinerea bacterial strains GXGL-7 and GXNN-7 are respectively up to 100%, the inhibition rate on GXNN-6 is 96.21%, the inhibition rates on the multi-bacterium sensitive botrytis cinerea bacterial strains HBLF-10 and GXZY-7 are respectively up to 100%, and the inhibition rate on GXZY-4 is up to 97.01%.
Furthermore, the alcohol extract has obvious inhibiting effect on OTA (over the air) generated by carbon black aspergillosis, and when the concentration of the alcohol extract is 5mg/mL, the OTA generation delay is most obvious
The invention has the beneficial effects that: the invention relates to a bactericidal medicament with asarum extract as an active ingredient, which is a botanical biological pesticide, belongs to one of biological medicaments, and meets the requirements of modern society on new pesticides. Secondly, the asarum extract has the characteristics of high antibacterial activity, wide antibacterial spectrum, difficult generation of resistance and no toxicity to people and animals. In addition, the aspergillus niger powder has an inhibiting effect on common pathogenic bacteria of grape fruits, has a remarkable inhibiting effect on OTA production capacity of aspergillus carbonarius, and can improve food safety of the grape fruits.
Detailed Description
The following detailed description is given with reference to preferred embodiments so that advantages and features of the present invention will be readily understood by those skilled in the art, and the scope of the present invention will be clearly and unequivocally defined.
Example 1:
preparation of aqueous and alcoholic extracts
The asarum of the present invention is purchased from the Anhui Bozhou medicinal material market. Drying the materials in the sun, pulverizing, and storing in a ventilated and dry place.
The method for extracting the water extract comprises the following steps: weighing 100g of pulverized and dried solanum nigrum, adding 1000mL of distilled water, and soaking for 30 min. Boiling with strong fire, decocting with slow fire, and extracting for 40 min. The liquid medicine is filtered with 4 layers of gauze while it is hot. Extracting once again, mixing 2 times of filtrates, and concentrating with slow fire to 1000 mg/mL. Storing at 4 deg.C for use. The alcohol extraction step of asarum comprises the following steps: weighing 800g of a crushed sample, soaking plant dry powder in 5 times of ethanol, and standing at room temperature for 12 h; ultrasonic extraction is carried out for 2 h; vacuum filtering, continuously extracting for 3 times, mixing filtrates, concentrating at 45 deg.C with rotary evaporator, drying, and storing at 4 deg.C.
Second, bacteriostatic culture and OTA detection method
The bacteriostatic activity of the asarum aqueous extract and the alcohol extract on botrytis cinerea (Botrytiscinerea), botrytis viticola (Coniothyrium diplodiella), botrytis cinerea (glomeriella cingulata) and Aspergillus carbonarius (Aspergillus carbonarius) is determined by adopting a hypha growth rate method. The method comprises the following specific steps:
activating strains, uniformly perforating the positions with strong hypha growth at the edge of the culture dish by using a sterile perforator (phi is 5mm) to obtain bacterial cakes, inoculating the bacterial cakes onto a culture medium, inoculating one bacterial cake to the center of each culture dish, inversely culturing for 2-7 days in an incubator at the temperature of 25-28 ℃ and the humidity of 73%, measuring the diameter of the bacterial colonies by using a cross method, and calculating the inhibition rate. Each treatment was set to 3 replicates.
The strain was cultured on CYA medium for 14d, and 12 pieces of the cell-carrying culture were punched out daily with a sterile punch (. phi. ═ 5 mm). Placing the bacteria-carrying culture in a 5mL small test tube, weighing, adding 2mL of methanol, shaking in a vortex for 1min, standing for 60min, filtering with a 0.45 μm filter membrane, filtering with a 0.22 μm filter membrane, and detecting with an ultra-high performance liquid-tandem mass spectrometer.
Detection conditions of the ultra-high performance liquid-tandem mass spectrometer are as follows: the ultra high liquid chromatograph xevo TQ-S of Water in usa was used for programming and automatic sampling. The mobile phase is A1: chromatographic acetonitrile; a2: chromatographic methanol; b1: 0.2% formic acid water; b2: water; the flow rates were set as: 0.3 mL/min; sample introduction amount: 1 μ L. The toxin concentration per gram of culture was calculated from the mass of the agar block taken and the detected toxin content and expressed as toxin yield (ng/g).
Thirdly, bacteriostasis of the water extract and the alcohol extract
The result shows that the bacteriostasis rate of the asarum aqueous extract on white rot pathogen is 74.87%, the bacteriostasis effect is superior to that of anthrax and botrytis cinerea, and the asarum aqueous extract has no inhibition effect on carbon black aspergillosis.
The asarum alcohol extract has a good bacteriostatic effect on 4 grape pathogenic fungi. When the concentration of the asarum alcohol extract is 5mg/mL, the inhibition rate of the asarum alcohol extract on botrytis cinerea is 97.13%, and the inhibition rate on white rot, colletotrichum and carbon black aspergillosis is up to 100%. When the concentration of the alcohol extract is 2.5mg/mL, the bacteriostasis rate to 4 pathogenic bacteria is above 85.5 percent, and the EC50 value is 0.48-1.10 mg/mL. The asarum alcohol extract has the best bacteriostatic effect on white rot fungi, and when the alcohol extract concentration is 1.25mg/mL, the suppression rate on white rot fungi is as high as 98.08%.
Fourth, the inhibiting effect of the alcohol extract on multi-bacterium sensitive anthrax bacteria strains
When the concentration of the asarum alcohol extract is 5mg/mL, the bacteriostasis rates of multi-bacterium sensitive anthrax bacteria strains FJZZ-62, coll-9 and coll-20 are respectively 96.30 percent, 94.33 percent and 96.88 percent, and the EC50 of the asarum alcohol extract is respectively 0.52, 0.92 and 0.46 mg/mL.
Fifthly, the inhibiting effect of the alcohol extract on carbendazim strain with high resistance to colletotrichum
The asarum alcohol extract has good inhibition effect on carbendazim strain with high resistance to colletotrichum. When the concentration of the alcohol extract is 5mg/mL, the inhibition rates of the carbendazim on high-resistance anthrax bacteria strains FJND-9 and GZSD-89 are both up to 100%, and the inhibition rate of the carbendazim on FJND-40 is also up to 96.09%. EC50 was 0.46, 1.39, and 0.77mg/mL, respectively. And the inhibition rates of carbendazim of 100 mu g/mL on FJND-9, FJND-40 and GZSD-89 strains are respectively 9.80%, 21.50% and 10.01%. When the concentration of the alcohol extract is 0.625mg/mL, the inhibitory activity of the alcohol extract on high-antibacterial strains is also obviously stronger than that of carbendazim of 100 mu g/mL.
Sixthly, inhibiting effect of alcohol extract on multiple sensitive botrytis cinerea strains
When the concentration of the asarum alcohol extract is 5mg/mL, the bacterial inhibition rates of the asarum alcohol extract on multiple sensitive botrytis cinerea strains GXGL-7 and GXNN-7 are both up to 100%, the bacterial inhibition rate on GXNN-6 is 96.21%, and the EC50 is 1.06-1.59 mg/mL.
Seventhly, inhibiting effect of alcohol extract on carbendazim high-resistance gray mold bacterial strain
The inhibition rate of carbendazim with the concentration of 100 mu g/mL on HBLF-10, GXZY-4 and GXZY-7 strains is 53.45%, 55.11% and 14.76% respectively. The asarum alcohol extract has a good inhibition effect on the carbendazim high-resistance botrytis cinerea strains, and the effect is stronger than that of the carbendazim of 100 mu g/mL. When the concentration is 5mg/mL, the inhibition rates of the carbendazim for the high-resistance botrytis cinerea strains HBLF-10 and GXZY-7 are both up to 100 percent, and the inhibition rate of the carbendazim for the GXZY-4 is 97.01 percent. The EC50 is 1.04-1.34 mg/mL.
Eighthly, inhibiting effect of alcohol extract on toxin production of aspergillus niger
In the blank, OTA was produced starting at 3d and at a concentration of 6.22ng/g, and OTA production peaked at 9d at a concentration of 2970.78 ng/g. When asarum alcohol extract was added to the medium, OTA production was retarded compared to the blank control. The higher the asarum alcohol extract concentration, the later the OTA production time is, and the less toxin is produced. At the concentration of 0.3125mg/mL, OTA began to be produced at 5d, and the concentration of OTA was 196.44 ng/g. At 5mg/mL, the OTA yield lags the most obviously, only a small amount of OTA is produced at 9d, the OTA concentration is 31.56ng/g, and 563.78ng/g at 14d, which is obviously lower than that of the control.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (5)
1. An application of asarum extract as plant antipathogen is characterized in that: the asarum extract is an aqueous extract or an alcohol extract, and the aqueous extract extraction method comprises the following steps: weighing 100g of dried crushed asarum, adding 1000mL of distilled water, soaking for 30min, boiling with strong fire, decocting with slow fire for 40min, filtering the liquid medicine with 4 layers of gauze while the liquid medicine is hot, repeatedly extracting for one time, combining 2 times of filtrates, concentrating with slow fire to a concentration of 1000mg/mL, and storing at 4 ℃ for later use; the method for extracting the alcohol extract comprises the following steps: weighing 800g of a crushed sample, soaking the plant dry powder in 5 times of ethanol, and standing at room temperature for 12 h; ultrasonic extraction is carried out for 2 h; vacuum filtering, continuously extracting for 3 times, mixing filtrates, concentrating at 45 deg.C with rotary evaporator, drying, and storing at 4 deg.C for use, wherein the pathogenic bacteria include Botrytis cinerea, Colletotrichum viticola and Aspergillus niger.
2. The use of claim 1, wherein said colletotrichum vitis is comprised of a highly carbendazim resistant strain of colletotrichum and said botrytis cinerea is comprised of a highly carbendazim resistant strain of botrytis cinerea.
3. The use of claim 2, wherein the aqueous extract concentration is 6.25mg/mL to 100mg/mL and the alcoholic extract concentration is 0.3125mg/mL to 5 mg/mL.
4. The use according to claim 3, wherein the inhibition rates for Colletotrichum anthracis, white rot, and Botrytis cinerea are 40.40%, 74.87%, and 37.50%, respectively, when the aqueous extract is 100 mg/mL; when the concentration of the alcohol extract is 5mg/mL, the inhibition rate on botrytis cinerea is 97.13%, and the inhibition rate on white rot, colletotrichum and carbon black aspergillosis is up to 100%; when the concentration of the alcohol extract is 2.5mg/mL, the bacteriostasis rate to 4 pathogenic bacteria is over 85.5 percent; when the concentration of the alcohol extract is 5mg/mL, the inhibition rates of the alcohol extract on multi-bacterium sensitive colletotrichum anthracnose bacterial strains FJZZ-62, coll-9 and coll-20 are respectively 96.30%, 94.33% and 96.88%, the inhibition rates on carbendazim high-resistant colletotrichum anthracnose bacterial strains FJND-9 and GZSD-89 are respectively up to 100%, the inhibition rate on FJND-40 is also up to 96.09%, the inhibition rates on multi-bacterium sensitive botrytis cinerea bacterial strains GXGL-7 and GXNN-7 are respectively up to 100%, the inhibition rate on GXNN-6 is 96.21%, the inhibition rates on the multi-bacterium sensitive botrytis cinerea bacterial strains HBLF-10 and GXZY-7 are respectively up to 100%, and the inhibition rate on GXZY-4 is up to 97.01%.
5. The use of claim 4, wherein the alcohol extract has significant inhibitory effect on OTA production by Aspergillus carbonarius, and the OTA production lag is most significant when the alcohol extract concentration is 5 mg/mL.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101027990A (en) * | 2007-03-13 | 2007-09-05 | 西北农林科技大学无公害农药研究服务中心 | Bactericidal use of asarone for preventing and treating plant diseases caused by plant pathogenic bacteria |
| CN105941513A (en) * | 2016-06-26 | 2016-09-21 | 合肥慧谷农业科技有限公司 | Plant bactericide and preparation method thereof |
-
2017
- 2017-12-13 CN CN201711325207.6A patent/CN108142443B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101027990A (en) * | 2007-03-13 | 2007-09-05 | 西北农林科技大学无公害农药研究服务中心 | Bactericidal use of asarone for preventing and treating plant diseases caused by plant pathogenic bacteria |
| CN105941513A (en) * | 2016-06-26 | 2016-09-21 | 合肥慧谷农业科技有限公司 | Plant bactericide and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 细辛挥发油抗植物病原真菌活性及作用机理研究;刘海燕;《中国优秀博硕士学位论文全文数据库 (硕士)医药卫生科技辑》;20061115(第11期);第24-26页 * |
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| CN108142443A (en) | 2018-06-12 |
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