CN108142333A - A kind of hirudiniculture method - Google Patents
A kind of hirudiniculture method Download PDFInfo
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- CN108142333A CN108142333A CN201711348527.3A CN201711348527A CN108142333A CN 108142333 A CN108142333 A CN 108142333A CN 201711348527 A CN201711348527 A CN 201711348527A CN 108142333 A CN108142333 A CN 108142333A
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 241000545744 Hirudinea Species 0.000 claims abstract description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 11
- 239000002985 plastic film Substances 0.000 claims abstract description 5
- 229920006255 plastic film Polymers 0.000 claims abstract description 5
- 238000009313 farming Methods 0.000 claims abstract description 4
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 238000003860 storage Methods 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 12
- 102000006395 Globulins Human genes 0.000 claims description 9
- 108010044091 Globulins Proteins 0.000 claims description 9
- 150000003839 salts Chemical class 0.000 claims description 9
- 241000196324 Embryophyta Species 0.000 claims description 7
- 239000004475 Arginine Substances 0.000 claims description 6
- 241000522254 Cassia Species 0.000 claims description 6
- 244000111489 Gardenia augusta Species 0.000 claims description 6
- 235000018958 Gardenia augusta Nutrition 0.000 claims description 6
- 244000153234 Hibiscus abelmoschus Species 0.000 claims description 6
- 244000062241 Kaempferia galanga Species 0.000 claims description 6
- 235000013421 Kaempferia galanga Nutrition 0.000 claims description 6
- 241000570011 Pomacea canaliculata Species 0.000 claims description 6
- 244000223014 Syzygium aromaticum Species 0.000 claims description 6
- 235000016639 Syzygium aromaticum Nutrition 0.000 claims description 6
- 235000019742 Vitamins premix Nutrition 0.000 claims description 6
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 6
- 210000004369 blood Anatomy 0.000 claims description 6
- 239000008280 blood Substances 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 238000005057 refrigeration Methods 0.000 claims description 6
- 240000002045 Guettarda speciosa Species 0.000 claims description 5
- 235000001287 Guettarda speciosa Nutrition 0.000 claims description 5
- 241001465754 Metazoa Species 0.000 claims description 4
- RXZBMPWDPOLZGW-XMRMVWPWSA-N (E)-roxithromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=N/OCOCCOC)/[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 RXZBMPWDPOLZGW-XMRMVWPWSA-N 0.000 claims description 3
- 208000019775 Back disease Diseases 0.000 claims description 3
- 102000004506 Blood Proteins Human genes 0.000 claims description 3
- 108010017384 Blood Proteins Proteins 0.000 claims description 3
- 241001133184 Colletotrichum agaves Species 0.000 claims description 3
- 240000003826 Eichhornia crassipes Species 0.000 claims description 3
- 102000009123 Fibrin Human genes 0.000 claims description 3
- 108010073385 Fibrin Proteins 0.000 claims description 3
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 claims description 3
- 241000248484 Ichthyophthirius Species 0.000 claims description 3
- 235000002710 Ilex cornuta Nutrition 0.000 claims description 3
- 241001310146 Ilex cornuta Species 0.000 claims description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
- 244000207740 Lemna minor Species 0.000 claims description 3
- 235000006439 Lemna minor Nutrition 0.000 claims description 3
- 235000010326 Osmanthus heterophyllus Nutrition 0.000 claims description 3
- 235000001855 Portulaca oleracea Nutrition 0.000 claims description 3
- 229910000831 Steel Inorganic materials 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 239000003146 anticoagulant agent Substances 0.000 claims description 3
- 229940127219 anticoagulant drug Drugs 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 230000006806 disease prevention Effects 0.000 claims description 3
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- 239000000835 fiber Substances 0.000 claims description 3
- 229950003499 fibrin Drugs 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims description 3
- 230000007774 longterm Effects 0.000 claims description 3
- 210000002381 plasma Anatomy 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 229960005224 roxithromycin Drugs 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 239000010959 steel Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 230000003442 weekly effect Effects 0.000 claims description 3
- BCZXFFBUYPCTSJ-UHFFFAOYSA-L Calcium propionate Chemical compound [Ca+2].CCC([O-])=O.CCC([O-])=O BCZXFFBUYPCTSJ-UHFFFAOYSA-L 0.000 claims description 2
- 239000004033 plastic Substances 0.000 claims description 2
- 229920003023 plastic Polymers 0.000 claims description 2
- 238000001471 micro-filtration Methods 0.000 claims 1
- 201000010099 disease Diseases 0.000 abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 5
- 239000002689 soil Substances 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 2
- 241000213006 Angelica dahurica Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
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- 239000004330 calcium propionate Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 238000003359 percent control normalization Methods 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/40—Culture of aquatic animals of annelids, e.g. lugworms or Eunice
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of hirudiniculture methods, include the following steps：(1) farming site selection and preparation：Excavate at least one long 100m on the ground, wide 1m, the pond of depth 1m, basin bottom setting drainage pipeline carries out disinfection with 0.5% sodium hydroxide, be laid with plastic film in basin bottom after disinfection and cover one layer of soil bed on a plastic film；Water storage；Water plant, the anti-wall of escaping of sink top setting are planted in the pond top, and pond top is provided with solar protection devices, and pond periphery should be at least connected with one piece of shady and cool moist place.The present invention provides a kind of hirudiniculture methods of science so that leech quantum of output is high, reduces the disease during hirudiniculture.
Description
Technical field
The present invention relates to cultural technique field, more particularly to a kind of hirudiniculture technology.
Background technology
With scientific development, the medical value of leech is increasingly valued by people, with social development, the open country of leech
Production-goods source increasingly depleted, the artificial breeding of leech lack one into a kind of current important development trend, however in the prior art
The hirudiniculture method of kind science so that leech quantum of output is high, reduces the disease during hirudiniculture.
Therefore those skilled in the art are dedicated to developing a kind of hirudiniculture method of science so that and leech quantum of output is high,
Reduce the disease during hirudiniculture.
Invention content
In view of the drawbacks described above of the prior art, the technical problems to be solved by the invention are to provide a kind of leech of science
Cultural method so that leech quantum of output is high, reduces the disease during hirudiniculture.
Realize above-mentioned purpose, the present invention provides a kind of hirudiniculture methods, include the following steps：
(1) farming site selection and preparation：At least one long 100m, wide 1m, the pond of depth 1m, pond are excavated on the ground
Bottom sets drainage pipeline, is carried out disinfection with 0.5% sodium hydroxide, is laid with plastic film in basin bottom after disinfection and is moulding
Expect the native bed of one layer of covering on film；Water storage；Plant water plant, the anti-wall of escaping of sink top setting, pond top in the pond top
Solar protection devices is provided with, pond periphery should be at least connected with one piece of shady and cool moist place.
(2) leech is launched：After introducing a fine variety, leech is thrown to shady and cool moist place in step (1), leech is waited for climb naturally
Pond is advanced into, each pond cultivates 2000-3000 leech.
(3) leech is raised：By the way of man-made feeds and natural feed, the natural feed is Pomacea canaliculata, often
Launch 50-53 kilograms of Pomacea canaliculata in a pond；Weekly launch a man-made feeds, man-made feeds launch 48 hours after pull out not by into
The feed of food.
(4) disease prevention and cure：The roxithromycin that man-made feeds in the step (3) participate in 0.5% is raised to prevent
Leech group's enterogastritis.
Change water is put into 0.5% salt to prevent leech ichthyophthirius, the die back disease of harnessing the river in pond every time.
Preferably, the water plant in the step (1) is water hyacinth and duckweed.
Preferably, prevent escaping in the step (1) being set as 80-90cm by force.
Preferably, in the step (1), the surface of the anti-wall of escaping is set as coarse metope.
Preferably, the man-made feeds by weight, including following ingredient：100-150 parts of globulin powder, arginine
0.35-0.38 parts, 15-20 parts of salt is mostly used 0.3-0.5 parts of vitamin premix, 2-5 parts of dried orange peel of 0.2-0.5 parts of Moschus, cloves
2-5 parts, 2-5 parts of Chinese cassia tree, 2-3 parts of the root of Dahurain angelica, 1-2 parts of cape jasmine, 1-2 parts of kaempferia galanga, 1-2 parts of asafoetide.
Preferably, the globulin powder is prepared in accordance with the following methods：
61) animal blood is taken to be put into rustless steel container, does not stop to stir in the same direction with the thick wooden stick for being tied with disinfection bandage
It mixes, until removing fibrin.
62) it is possible to be subjected to remaining fiber short slag and the external world in coarse filtration removing blood into liquid with 40 mesh filter clothes for step 61)
The impurity brought into.
63) step 62) product is proportionally added into 0.5% anti-coagulants Chinese holly edge acid sodium, adds in 0.2%-0.5% antistaling agents third
Sour calcium, spare in 2-4 DEG C of refrigeration after full and uniform, long-term preserve need to seal refrigeration under the conditions of -18 DEG C.
64) by step 63) product, using liquid-liquid separation machine, two phases were separated, centrifuge speed 1800-2000r/min,
Time is 3-5 minutes.
65) by step 64) product using 5-25 DEG C of temperature, pressure 0.15-0.45Mpa, 5-15 μm of filtration media pore diameters into
Row hyperfiltration.
66) the concentration blood plasma in step 65) is spray-dried, manufactures plasma protein powder.
Preferably, aforementioned artificial leech feed producing method, includes the following steps：
71) Moschus, dried orange peel, cloves, Chinese cassia tree, the root of Dahurain angelica, cape jasmine, kaempferia galanga, asafoetide by weight is taken, adds decocting 3-4
Hour, filter to take to obtain liquid.
72) globulin powder by weight, arginine are taken, salt is mostly used vitamin premix and adds in step 71)
Traditional Chinese medicine liquid, drying are granulated, and obtain feed.
The beneficial effects of the invention are as follows：The present invention provides a kind of hirudiniculture methods of science so that leech quantum of output
Height reduces the disease during hirudiniculture.
Specific embodiment
With reference to embodiment, the invention will be further described：
Embodiment one, selection leech 2000 follow the steps below cultivation：
(1) farming site selection and preparation：A long 100m, wide 1m, the pond of depth 1m, basin bottom are excavated on the ground
Set drainage pipeline, carried out disinfection with 0.5% sodium hydroxide, after disinfection basin bottom be laid with plastic film and in plastics it is thin
One layer of native bed is covered on film；Water storage；Water plant is planted on the pond top, and the anti-wall of escaping of sink top setting, pond top is set
There is solar protection devices, pond periphery should be at least connected with one piece of shady and cool moist place.
(2) leech is launched：After introducing a fine variety, leech is thrown to shady and cool moist place in step (1), leech is waited for climb naturally
Pond is advanced into, each pond cultivates 2000 leech.
(3) leech is raised：By the way of man-made feeds and natural feed, the natural feed is Pomacea canaliculata, often
Launch 50 kilograms of Pomacea canaliculatas in a pond；A man-made feeds are launched weekly, and man-made feeds are pulled out after launching 48 hours and do not fed
Feed.
(4) disease prevention and cure：The roxithromycin that man-made feeds in the step (3) participate in 0.5% is raised to prevent
Leech group's enterogastritis；
Change water is put into 0.5% salt to prevent leech ichthyophthirius, the die back disease of harnessing the river in pond every time.
Preferably, the water plant in the step (1) is water hyacinth and duckweed.
Preferably, anti-wall of escaping is set as 80cm in the step (1).
It escapes when wall is set as 80-90cm when anti-and can farthest prevent cultivated leech from escaping, other implementations
In mode, it is identical to achieve the effect that the wall that anti-can will also escape be set as the other values in 80-90cm, such as 81cm, 82cm,
83cm, 84cm, 85cm, 86cm, 87cm, 88cm, 89cm, 90cm.
Preferably, in the step (1), the surface of the anti-wall of escaping is set as coarse metope.
Preferably, the man-made feeds by weight, including following ingredient：100-150 parts of globulin powder, arginine
0.35 part, 15 parts of salt, multi-purpose 0.3 part of vitamin premix, 2 parts of dried orange peel of 0.2-0. parts of Moschus, 2 parts of cloves, 2 parts of Chinese cassia tree, in vain
2 parts of the root of Dahurian angelica, 1 part of cape jasmine, 1 part of kaempferia galanga, 1 part of asafoetide.
Preferably, the globulin powder is prepared in accordance with the following methods：
61) animal blood is taken to be put into rustless steel container, does not stop to stir in the same direction with the thick wooden stick for being tied with disinfection bandage
It mixes, until removing fibrin；
62) it is possible to be subjected to remaining fiber short slag and the external world in coarse filtration removing blood into liquid with 40 mesh filter clothes for step 61)
The impurity brought into；
63) step 62) product is proportionally added into 0.5% anti-coagulants Chinese holly edge acid sodium, adds in 0.5% antistaling agent calcium propionate,
Spare in 2-4 DEG C of refrigeration after full and uniform, long-term preserve need to seal refrigeration under the conditions of -18 DEG C；
64) by step 63) product, using liquid-liquid separation machine, two phases were separated, centrifuge speed 1800-2000r/min,
Time is 3-5 minutes；
65) by step 64) product using 5-25 DEG C of temperature, pressure 0.15-0.45Mpa, 5-15 μm of filtration media pore diameters into
Row hyperfiltration；
66) the concentration blood plasma in step 65) is spray-dried, manufactures plasma protein powder.
Preferably, aforementioned artificial leech feed producing method, includes the following steps：
71) Moschus, dried orange peel, cloves, Chinese cassia tree, the root of Dahurain angelica, cape jasmine, kaempferia galanga, asafoetide by weight is taken, adds decocting 3-4
Hour, filter to take to obtain liquid；
72) globulin powder by weight, arginine are taken, salt is mostly used vitamin premix and adds in step 71)
Traditional Chinese medicine liquid, drying are granulated, and obtain feed.
Comparative example：On April 1st, 2016, leech 4000 of fetching water are divided into two groups, and first group is test group, according to implementation
The method of example one is cultivated, and second group is control group, is raised according to normal method, after two months, compares its rate of animals delivered to the slaughter-house
And the rate of output, obtain 1 result of table：
| Group | The death rate | Average weight growth rate |
| Test group | 8% | 400% |
| Control group | 12% | 392% |
By it is found that the leech raised using this method, death rate smaller, weight gain bigger.
The preferred embodiment of the present invention described in detail above.It should be appreciated that those of ordinary skill in the art without
Creative work is needed according to the present invention can to conceive and makes many modifications and variations.Therefore, all technologies in the art
Personnel are available by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Technical solution, all should be in the protection domain being defined in the patent claims.
Claims (7)
1. a kind of hirudiniculture method, it is characterized in that：Include the following steps：
(1) farming site selection and preparation：At least one long 100m, wide 1m, the pond of depth 1m, basin bottom are excavated on the ground
Set drainage pipeline, carried out disinfection with 0.5% sodium hydroxide, after disinfection basin bottom be laid with plastic film and in plastics it is thin
One layer of native bed is covered on film；Water storage；Water plant is planted on the pond top, and the anti-wall of escaping of sink top setting, pond top is set
There is solar protection devices, pond periphery should be at least connected with one piece of shady and cool moist place.
(2) leech is launched：After introducing a fine variety, leech is thrown in step (1) shady and cool moist place, wait for leech creep naturally into
Enter pond, each pond cultivates 2000-3000 leech.
(3) leech is raised：Using by the way of man-made feeds and natural feed, the natural feed is Pomacea canaliculata, Mei Geshui
Launch 50-53 kilograms of Pomacea canaliculata in pond；A man-made feeds are launched weekly, and man-made feeds pull what is do not fed out after launching 48 hours
Feed.
(4) disease prevention and cure：The roxithromycin that man-made feeds in the step (3) participate in 0.5% is raised to prevent leech of harnessing the river
Group's enterogastritis；
Change water is put into 0.5% salt to prevent leech ichthyophthirius, the die back disease of harnessing the river in pond every time.
2. hirudiniculture method as described in claim 1, it is characterized in that:Water plant in the step (1) for water hyacinth and floats
Duckweed.
3. hirudiniculture method as described in claim 1, it is characterized in that:Prevent escaping in the step (1) being set as 80- by force
90cm。
4. hirudiniculture method as described in claim 1, it is characterized in that:In the step (1), the surface of the anti-wall of escaping
It is set as coarse metope.
5. hirudiniculture method as described in claim 1, it is characterized in that：The man-made feeds by weight, including as follows
Ingredient：100-150 parts of globulin powder, 0.35-0.38 parts of arginine, 15-20 parts of salt are mostly used vitamin premix 0.3-
0.5 part, 2-5 parts of dried orange peel of 0.2-0.5 parts of Moschus, 2-5 parts of cloves, 2-5 parts of Chinese cassia tree, 2-3 parts of the root of Dahurain angelica, 1-2 parts of cape jasmine, kaempferia galanga 1-2
Part, 1-2 parts of asafoetide.
6. hirudiniculture method as claimed in claim 5, it is characterized in that：The globulin powder is made in accordance with the following methods
It is standby：
61) animal blood is taken to be put into rustless steel container, is kept stirring in the same direction with the thick wooden stick for being tied with disinfection bandage, directly
Until fibrin is removed；
62) by step 61) into liquid, carrying out remaining fiber short slag and the external world in coarse filtration removing blood with 40 mesh filter clothes may bring into
Impurity；
63) step 62) product is proportionally added into 0.5% anti-coagulants Chinese holly edge acid sodium, adds in 0.2%-0.5% antistaling agent propionic acid
Calcium, spare in 2-4 DEG C of refrigeration after full and uniform, long-term preserve need to seal refrigeration under the conditions of -18 DEG C；
64) by step 63) product, using liquid-liquid separation machine, two phases were separated, centrifuge speed 1800-2000r/min, the time
It is 3-5 minutes；
65) step 64) product is used into 5-25 DEG C of temperature, pressure 0.15-0.45Mpa, 5-15 μm of filtration media pore diameters are surpassed
Microfiltration；
66) the concentration blood plasma in step 65) is spray-dried, manufactures plasma protein powder.
7. a kind of artificial leech feed producing method as claimed in claim 5, includes the following steps：
71) Moschus, dried orange peel, cloves, Chinese cassia tree, the root of Dahurain angelica, cape jasmine, kaempferia galanga, asafoetide by weight is taken, adds decocting 3-4 hours,
Filter to take to obtain liquid；
72) globulin powder by weight, arginine are taken, salt is mostly used the Chinese medicine that vitamin premix adds in step 71)
Liquid, drying are granulated, and obtain feed.
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| CN201711348527.3A CN108142333A (en) | 2017-12-15 | 2017-12-15 | A kind of hirudiniculture method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711348527.3A CN108142333A (en) | 2017-12-15 | 2017-12-15 | A kind of hirudiniculture method |
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Cited By (5)
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| CN108719152A (en) * | 2018-08-24 | 2018-11-02 | 合肥启迪农业发展有限公司 | A kind of cultural method of leech |
| CN108901954A (en) * | 2018-08-09 | 2018-11-30 | 合肥康青源养殖有限公司 | A kind of hirudiniculture method |
| CN109122427A (en) * | 2018-08-09 | 2019-01-04 | 合肥康青源养殖有限公司 | Improve the cultural method of leech disease resistance |
| CN109452208A (en) * | 2018-12-21 | 2019-03-12 | 涂元清 | The method of a kind of ground membrane type high-density breeding leech |
| CN113475624A (en) * | 2021-06-10 | 2021-10-08 | 云南农业大学 | Poecilobdella manillensis compound feed |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108901954A (en) * | 2018-08-09 | 2018-11-30 | 合肥康青源养殖有限公司 | A kind of hirudiniculture method |
| CN109122427A (en) * | 2018-08-09 | 2019-01-04 | 合肥康青源养殖有限公司 | Improve the cultural method of leech disease resistance |
| CN108719152A (en) * | 2018-08-24 | 2018-11-02 | 合肥启迪农业发展有限公司 | A kind of cultural method of leech |
| CN109452208A (en) * | 2018-12-21 | 2019-03-12 | 涂元清 | The method of a kind of ground membrane type high-density breeding leech |
| CN113475624A (en) * | 2021-06-10 | 2021-10-08 | 云南农业大学 | Poecilobdella manillensis compound feed |
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