CN108130269A - Plant-derived material DNA extraction elements and extracting method - Google Patents
Plant-derived material DNA extraction elements and extracting method Download PDFInfo
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- CN108130269A CN108130269A CN201711457291.7A CN201711457291A CN108130269A CN 108130269 A CN108130269 A CN 108130269A CN 201711457291 A CN201711457291 A CN 201711457291A CN 108130269 A CN108130269 A CN 108130269A
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- selection
- winnowing cylinder
- winnowing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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Abstract
The invention discloses a kind of plant-derived material DNA extraction elements and extracting method, including：Air classifier, air classifier include selection by winnowing cylinder and the mill for being arranged on selection by winnowing cylinder lower part, and selection by winnowing cylinder bottom end is also associated with wind turbine, and the clast that mill is ground can be blown into selection by winnowing cylinder by wind turbine；Selection by winnowing cylinder top is blocked by filter bed, and at least one back flow plate tilted upward is equipped in the inner cavity of selection by winnowing cylinder vertical portion, is close to be provided with return port on the inner wall of the selection by winnowing cylinder at the upper surface of back flow plate, return port passes through conveying pipeline unicom mill.The present invention will be ground and selection by winnowing is combined into one, the particle size packaging material finally needed can be collected by selection by winnowing spare, pass through the effect of back flow plate and conveying pipeline etc. simultaneously, as long as can by the larger undesirable clast of particle flow back into mill repeat grinding its meet the requirements, present invention may apply to room temperature grindings, and it can remove from largely grinding demand, the short slab of manpower grinding.
Description
Technical field
The present invention relates to a kind of gene extractive technique more particularly to a kind of plant-derived material DNA extraction elements and extractions
Method.
Background technology
The environmental problem brought with food-safety problem and food packaging is paid close attention to by various circles of society, is eaten as health
Product packaging and environmentally safe edible packaging progress into the visual field of people.Edible packaging in plant-derived material
Refer to meet the functional requirements such as complete protection food appearance, health, safety, and to environment and the free of contamination packaging of food, that is, wrap
Material therefor, technique, equipment are filled all to environment and food no pollution.Scholar both domestic and external, expert are one after another to its performance and system
Standby mode is innovated, is improved, make it is more practical, conveniently.Since edible packaging quickly develops, safety also obtains
It is extensive to pay attention to.As the food using plant as Raw material processing, safety is unclear, therefore, it is necessary to commercial plant
Transgene component in source property edible packing material is detected, and situation and long-term consumption safety are taken in assess its crowd
Background information is provided.
Report in relation to transgenic foods safety has caused world wide internal medicine scholar and the public and transgenic product has been pacified
The extensive concern of full property.The safety of transgenic product is all the time just controversial, therefore GMO detections are with particularly significant
Meaning.What ministry of Health of China was promulgated《GM food administration of health method》Also require to GM food edible safety and
Nutritional quality is evaluated.The country has issued many relevant national standards and professional standard, corresponding detection method are non-
It is often ripe.Mostly to detect based on exogenous DNA, fluorescence PCR method is widely used in transgenosis inspection for the detection of transgene component
It surveys.Has pertinent literature report, to the detection GMOs of plant and its converted products, however to plant source edibility packet in the market
Package material detection of GMOs there is no literature reported on.The present invention collects all kinds of wafers on sale in the market, plant capsule, egg
The plant sources edible packing materials such as cylinder are sample, carry out detection of GMOs.
Invention content
The present invention overcomes the deficiencies in the prior art, provide a kind of plant-derived material DNA extraction elements and extracting method.
In order to achieve the above objectives, a kind of technical solution for using of the present invention for：A kind of plant-derived material DNA extractions dress
It puts, including：Air classifier, the air classifier include selection by winnowing cylinder and the mill for being arranged on selection by winnowing cylinder lower part, the selection by winnowing cylinder bottom end
Wind turbine is also associated with, the clast that mill is ground can be blown into the selection by winnowing cylinder by the wind turbine；The selection by winnowing cylinder top passes through
Filter bed blocks, and at least one back flow plate tilted upward is equipped in the inner cavity of the selection by winnowing cylinder vertical portion, is close to the reflux
Return port is provided on the inner wall of selection by winnowing cylinder at the upper surface of plate, the return port passes through mill described in conveying pipeline unicom.
In a preferred embodiment of the present invention, the back flow plate bottom margin connects the selection by winnowing cylinder inner wall.
In a preferred embodiment of the present invention, the selection by winnowing cylinder is made of transparent glass material.
In a preferred embodiment of the present invention, the extraction element further includes isolation cover, and the air classifier is arranged on isolation
In cover.
In a preferred embodiment of the present invention, tip portion of the selection by winnowing cylinder with filter bed is horizontally disposed with.
In a preferred embodiment of the present invention, the filter bed is interwoven by glass fibre.
In a preferred embodiment of the present invention, the filter bed is provided with two layers of alternate setting, close to selection by winnowing cylinder port
The glass fibre tight ness rating of filter bed is more than the glass fibre tight ness rating of the filter bed far from selection by winnowing cylinder port.
In a preferred embodiment of the present invention, three pieces of upper and lower alternate setting are provided in the vertical portion of the selection by winnowing cylinder
Back flow plate, every piece of back flow plate are correspondingly arranged a return port.
In a preferred embodiment of the present invention, the mill includes two grinding bases being mutually close together, at least
Water cooling coil pipe is equipped in one grinding base.
The another technical solution that the present invention uses for：A kind of extracting method carried out using DNA extraction elements, including with
Lower step：
S1 pours into the plant-derived material of preliminary crushing into particle in selection by winnowing cylinder by the opening at the top of selection by winnowing cylinder, then uses
Filter bed blocks the opening at the top of selection by winnowing cylinder；
S2 opens mill and wind turbine, makes the wind that wind turbine is blown out that plant-derived material be driven to be recycled in mill and selection by winnowing cylinder,
Particle the most tiny simultaneously is hidden attached by filter bed；
S3 finally removes filter bed, by beaing that the plant-derived material collection for being ground into smalls is spare.
The present invention solves defect present in background technology, and the present invention has following advantageous effect：
（1）The packaging materials such as wafer, plant capsule, egg cylinder can be ground into clast by usually grinding setting, but in clast
The different clast of particle size is contained, is difficult to use subsequently in DNA extraction process, and grinding is closed two by the present invention with selection by winnowing
It is one, the particle size packaging material finally needed can be collected by selection by winnowing spare, while passes through back flow plate and defeated
The effect of expects pipe etc., if can by the larger undesirable clast of particle flow back into mill repeat grinding its meet
It is required that present invention may apply to room temperature grinding, and can remove from largely grinding demand, the short slab of manpower grinding（People
The clast grain size of power grinding is still not uniform enough）.
（2）By using the filter bed of two kinds of tight ness ratings, the smalls in the range of different-grain diameter can be hidden and be attached to corresponding filter
In pad, when in use, optimal smalls grain size can be selected according to demand.
（3）Glass fibre is mainly made of inorganic silica, and smalls will not be polluted, and will not influence to test
Data.
（4）By the effect of back flow plate, when the larger packaging material of particle enters back flow plate leeward, due to wind speed
Become smaller, will back flow into mill, and the more tiny packaging material of particle can be entered in filter bed with the wind.
（5）It is acted on by the reflux of three back flow plates, it is ensured that the packaging material for not having bulky grain enters filter bed
It is interior, while the size of back flow plate is sequentially increased from lower to upper, is ensured reflux effect gradually increase, can be increased selection by winnowing effect.
（6）Selection by winnowing cylinder and isolation cover can more clearly observe grinding and selection by winnowing situation so using glass material,
It is clean convenient for seeing whether before replacing grinding object material simultaneously, with anti-cross infection；Isolation cover can prevent wind turbine from inhaling
Take extraneous air, it is ensured that the air-supply of wind turbine is comparison totally free of contamination inner air.
Description of the drawings
The present invention is further described with reference to the accompanying drawings and examples；
Fig. 1 is the structure diagram of the preferred embodiment of the present invention；
In figure：1st, isolation cover；2nd, selection by winnowing cylinder；3rd, mill；4th, wind turbine；5th, back flow plate；6th, return port；7th, conveying pipeline；8th, first
Filter bed；9th, the second filter bed.
Specific embodiment
Presently in connection with drawings and examples, the present invention is described in further detail, these attached drawings are simplified signal
Figure only illustrates the basic structure of the present invention, therefore it only shows composition related to the present invention in a schematic way.
As shown in Figure 1, a kind of plant-derived material DNA extraction elements, including：Air classifier, air classifier include selection by winnowing cylinder and
The mill of selection by winnowing cylinder lower part is arranged on, selection by winnowing cylinder bottom end is also associated with wind turbine, and wind turbine can blow the clast that mill is ground
Enter selection by winnowing cylinder；Selection by winnowing cylinder top is blocked by filter bed, is tilted upward in the inner cavity of selection by winnowing cylinder vertical portion equipped at least three
Back flow plate is close to be provided with return port on the inner wall of the selection by winnowing cylinder at the upper surface of back flow plate, and return port passes through conveying pipeline unicom
Mill.
The packaging materials such as wafer, plant capsule, egg cylinder can be ground into clast by usually grinding setting, but in clast
The different clast of particle size is contained, is difficult to use subsequently in DNA extraction process, and grinding is closed two by the present invention with selection by winnowing
It is one, the particle size packaging material finally needed can be collected by selection by winnowing spare, while passes through back flow plate and defeated
The effect of expects pipe etc., if can by the larger undesirable clast of particle flow back into mill repeat grinding its meet
It is required that present invention may apply to room temperature grinding, and can remove from largely grinding demand, the short slab of manpower grinding（People
The clast grain size of power grinding is still not uniform enough）.
Back flow plate bottom margin connects selection by winnowing cylinder inner wall.By the effect of back flow plate, the larger packaging material of particle into
When entering to back flow plate leeward, since wind speed becomes smaller, it will back flow into mill, and the packaging that particle is more tiny
Material can be entered in filter bed with the wind.Three pieces of back flow plates of upper and lower alternate setting are provided in the vertical portion of selection by winnowing cylinder,
Every piece of back flow plate is correspondingly arranged a return port.It is acted on by the reflux of three back flow plates, it is ensured that do not have bulky grain
Packaging material enter in filter bed, while the size of back flow plate is sequentially increased from lower to upper, ensure reflux effect gradually increase,
Selection by winnowing effect can be increased.
Extraction element further includes isolation cover, and air classifier is arranged in isolation cover.Selection by winnowing cylinder and isolation cover use glass material
Matter can more clearly observe grinding and selection by winnowing situation in this way, while before replacing grinding object material, clear convenient for seeing whether
It is clean clean, with anti-cross infection；Isolation cover can prevent wind turbine suck up outside air, it is ensured that the air-supply of wind turbine is that comparison is clean
Free of contamination inner air.
Isolation cover selection transparent material is made, and has leakproofness, selection by winnowing cylinder is made of transparent glass material.
Tip portion of the selection by winnowing cylinder with filter bed is horizontally disposed with, and is hidden the attached smalls into filter bed in this way and will not be fallen easily
It falls.
Filter bed is interwoven by glass fibre, and filter bed is provided with two layers of alternate setting, the filter bed close to selection by winnowing cylinder port
For the first filter bed, the filter bed far from selection by winnowing cylinder port is the second filter bed, and the glass fibre tight ness rating of the filter bed of the first filter bed is more than
The glass fibre tight ness rating of second filter bed.It, can be by the smalls in the range of different-grain diameter by using the filter bed of two kinds of tight ness ratings
Tibetan is attached in corresponding filter bed, when in use, can select optimal smalls grain size according to demand.
Mill includes two grinding bases being mutually close together, and water cooling coil pipe is equipped at least one grinding base.It is logical
Integral outer cooling can be carried out, while water cooling coil pipe is by being passed through coolant media, Ke Yiyou to mill by crossing the blowing of wind turbine
Targetedly cool down to mill, ensure that mill will not be because of the DNA of high temperature smalls.
A kind of extracting method carried out using DNA extraction elements, is included the following steps：
S1 pours into the plant-derived material of preliminary crushing into particle in selection by winnowing cylinder by the opening at the top of selection by winnowing cylinder, then uses
Filter bed blocks the opening at the top of selection by winnowing cylinder；
S2 opens mill and wind turbine, makes the wind that wind turbine is blown out that plant-derived material be driven to be recycled in mill and selection by winnowing cylinder,
Particle the most tiny simultaneously is hidden attached by filter bed；
S3 finally removes filter bed, by beaing that the plant-derived material collection for being ground into smalls is spare.
By adjusting the wind speed of wind turbine, selection by winnowing particle can be adjusted, guarantees to hide the particle for being attached to the smalls in filter bed
Size meets the requirements.
If necessary to replace grinding selection by winnowing packaging material if, by wash and air-dry can thoroughly remove before it is residual
Stay in the residual packaging material in air classifier；, by being then turned on mill and wind turbine, it can also be made by being passed through glass sand simultaneously
Glass sand takes the residue remained in wind turbine and mill gap out of, puts subsequent cross-infection.
Desirable embodiment according to the present invention is enlightenment above, and by above-mentioned description, related personnel completely can be with
Without departing from the scope of the technological thought of the present invention', various changes and amendments are carried out.The technical scope of this invention
The content being not limited on specification, it is necessary to determine the technical scope according to the scope of the claims.
Claims (10)
1. a kind of plant-derived material DNA extraction elements, including：Air classifier, which is characterized in that the air classifier includes selection by winnowing cylinder
With the mill for being arranged on selection by winnowing cylinder lower part, the selection by winnowing cylinder bottom end is also associated with wind turbine, and the wind turbine can grind mill
The clast of mill is blown into the selection by winnowing cylinder；The selection by winnowing cylinder top is blocked by filter bed, in the inner cavity of the selection by winnowing cylinder vertical portion
Equipped at least one back flow plate tilted upward, it is close to be provided with back on the inner wall of the selection by winnowing cylinder at the upper surface of the back flow plate
Discharge orifice, the return port pass through mill described in conveying pipeline unicom.
2. a kind of plant-derived material DNA extraction elements according to claim 1, it is characterised in that：The back flow plate bottom
Portion edge connects the selection by winnowing cylinder inner wall.
3. a kind of plant-derived material DNA extraction elements according to claim 1, it is characterised in that：The selection by winnowing cylinder is
Transparent glass material is made.
4. a kind of plant-derived material DNA extraction elements according to claim 1, it is characterised in that：The extraction element
Isolation cover is further included, the air classifier is arranged in isolation cover.
5. a kind of plant-derived material DNA extraction elements according to claim 1, it is characterised in that：The mill packet
Two grinding bases being mutually close together are included, water cooling coil pipe is equipped at least one grinding base.
6. a kind of plant-derived material DNA extraction elements according to claim 1, it is characterised in that：The selection by winnowing cylinder band
The tip portion for having filter bed is horizontally disposed with.
7. a kind of plant-derived material DNA extraction elements according to claim 1, it is characterised in that：The filter bed is by glass
Glass fiber interweaving forms.
8. a kind of plant-derived material DNA extraction elements according to claim 1, it is characterised in that：The filter bed setting
Have two layers of alternate setting, the glass fibre tight ness rating close to the filter bed of selection by winnowing cylinder port is more than the filter bed far from selection by winnowing cylinder port
Glass fibre tight ness rating.
9. a kind of plant-derived material DNA extraction elements according to claim 1, it is characterised in that：The selection by winnowing cylinder
Three pieces of back flow plates of upper and lower alternate setting are provided in vertical portion, every piece of back flow plate is correspondingly arranged a return port.
10. the extracting method that the DNA extraction elements in a kind of 1-9 using claim described in any claim carry out, special
Sign is, includes the following steps：
S1 pours into the plant-derived material of preliminary crushing into particle in selection by winnowing cylinder by the opening at the top of selection by winnowing cylinder, then uses
Filter bed blocks the opening at the top of selection by winnowing cylinder；
S2 opens mill and wind turbine, makes the wind that wind turbine is blown out that plant-derived material be driven to be recycled in mill and selection by winnowing cylinder,
Particle the most tiny simultaneously is hidden attached by filter bed；
S3 finally removes filter bed, by beaing that the plant-derived material collection for being ground into smalls is spare.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711457291.7A CN108130269A (en) | 2017-12-28 | 2017-12-28 | Plant-derived material DNA extraction elements and extracting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711457291.7A CN108130269A (en) | 2017-12-28 | 2017-12-28 | Plant-derived material DNA extraction elements and extracting method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108130269A true CN108130269A (en) | 2018-06-08 |
Family
ID=62393323
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711457291.7A Pending CN108130269A (en) | 2017-12-28 | 2017-12-28 | Plant-derived material DNA extraction elements and extracting method |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3794251A (en) * | 1972-05-08 | 1974-02-26 | Williams Patent Crusher & Pulv | Material reducing system and apparatus |
| JP3767882B2 (en) * | 2000-05-26 | 2006-04-19 | 株式会社中山鉄工所 | Cyclone dust collector |
| CN201267805Y (en) * | 2008-08-28 | 2009-07-08 | 巨锋 | Fanning machine |
| CN203044344U (en) * | 2012-12-18 | 2013-07-10 | 上海季明环保设备有限公司 | Household garbage comprehensive sorting machine |
| CN203678465U (en) * | 2014-01-15 | 2014-07-02 | 王飞 | Lime grinder |
| CN205109773U (en) * | 2015-11-09 | 2016-03-30 | 武汉惠华三农种业有限公司 | Quick broken plant tissue's grinding machine |
-
2017
- 2017-12-28 CN CN201711457291.7A patent/CN108130269A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3794251A (en) * | 1972-05-08 | 1974-02-26 | Williams Patent Crusher & Pulv | Material reducing system and apparatus |
| JP3767882B2 (en) * | 2000-05-26 | 2006-04-19 | 株式会社中山鉄工所 | Cyclone dust collector |
| CN201267805Y (en) * | 2008-08-28 | 2009-07-08 | 巨锋 | Fanning machine |
| CN203044344U (en) * | 2012-12-18 | 2013-07-10 | 上海季明环保设备有限公司 | Household garbage comprehensive sorting machine |
| CN203678465U (en) * | 2014-01-15 | 2014-07-02 | 王飞 | Lime grinder |
| CN205109773U (en) * | 2015-11-09 | 2016-03-30 | 武汉惠华三农种业有限公司 | Quick broken plant tissue's grinding machine |
Non-Patent Citations (2)
| Title |
|---|
| 李根成: "《制粉工艺与设备》", 30 September 1994 * |
| 郭丰年: "《实用袋滤除尘技术》", 30 January 2015 * |
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Application publication date: 20180608 |
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