CN108118002A - A kind of horizontal stalk of branch is mould and its applies - Google Patents
A kind of horizontal stalk of branch is mould and its applies Download PDFInfo
- Publication number
- CN108118002A CN108118002A CN201810063402.4A CN201810063402A CN108118002A CN 108118002 A CN108118002 A CN 108118002A CN 201810063402 A CN201810063402 A CN 201810063402A CN 108118002 A CN108118002 A CN 108118002A
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- mould
- branch
- yeast
- stalk
- horizontal
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
Abstract
A kind of horizontal stalk of branch of offer of the present invention is mould, and preserving number is：CCTCC NO：2017696.The horizontal mould diacetyl content for being mixed with the distiller's yeast of brewed wine, can improving base liquor of stalk of the branch, promotes fragrance, to improve distillation yield and quality product rate.In addition, it can brew pure and sweet net refreshing, dense aroma distiller's yeast and its application and a kind of wine and its manufacturing method of pure and sweet net refreshing, dense aroma the present invention also provides the one kind.
Description
Technical field
The present invention relates to brewing technical fields, obstruct mould and its application in particular to a kind of branch is horizontal.
Background technology
Biacetyl, be under room temperature it is light yellow have benzoquinones smell to yellow-green liquid, weak solution has cream smell, is present in
In fennel oil and cream.Water is dissolved in, it can be miscible with ethyl alcohol, ether.It is mainly used as the increasing of cream, margarine, cheese and candy
The curing agent etc. of pastil, gelatin.A kind of substance for influencing drinks flavor, molecular formula CH3COCOCH3 is fermentation process
Middle yeast and the metabolite of bacterium.It is one of liquor flavor ingredient in white wine.At present, biacetyl in fragrant yeast
Ingredient is insufficient or unstable and cause wine product and vinosity difference.
The content of the invention
The first object of the present invention is to provide that a kind of horizontal stalk of branch is mould, and can increase makes base liquor ingredient keep basic
Stablize, improve distillation yield and quality product rate.
The second object of the present invention is in a kind of mould application in wine brewing of the horizontal stalk of branch of offer.
The third object of the present invention is to provide a kind of distiller's yeast, can improve distillation yield and quality product rate, brew and be rich in
The wine of aroma.
The fourth object of the present invention is the preparation method for providing above-mentioned distiller's yeast, and can produce can improve out wine
The distiller's yeast of rate and quality product rate.
The fifth object of the present invention is in the application in the above-mentioned distiller's yeast of offer in wine brewing.
The sixth object of the present invention is to provide a kind of brewing method, can brew it is pure and sweet net refreshing, rich in aroma
Wine.
The seventh object of the present invention is to provide one kind just, with the advantages of pure and sweet net refreshing, vinosity is high.
What the present invention was realized in：
A kind of horizontal stalk of branch is mould, and preserving number is：CCTCC NO：2017696.
Above-mentioned branch is horizontal to obstruct the mould application in wine brewing.
A kind of distiller's yeast, it is mould containing the horizontal stalk of above-mentioned branch.
The preparation method of above-mentioned distiller's yeast, including：It mixes the horizontal stalk of starter-making materials and above-mentioned branch is mould.
Application of the distiller's yeast in wine brewing, including following either step：
(1) during wine brewing, distiller's yeast is mixed with liquor-making raw material；
(2) pit mud cultivation with it is aging during, distiller's yeast is mixed with pit mud.
A kind of brewing method, including being mixed and being fermented the mould raw material with being used for wine brewing of the horizontal stalk of above-mentioned branch.
A kind of wine is made by above-mentioned brewing method.
The invention has the advantages that：
A kind of horizontal stalk of branch of offer of the present invention is mould, and preserving number is：CCTCC NO： 2017696.The horizontal stalk of the branch is mould
It is mixed with the distiller's yeast of brewed wine, the diacetyl content of base liquor can be improved, promote fragrance, to improve distillation yield and quality product rate.This
Outside, the present invention also provides the one kind, it can brew pure and sweet net refreshing, dense aroma distiller's yeast and its application and one kind is pure and sweet
The wine and its manufacturing method of net refreshing, dense aroma.
Description of the drawings
It in order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment
Attached drawing is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not to be seen as
It is the restriction to scope, it for those of ordinary skill in the art, without creative efforts, can be with
Other relevant attached drawings are obtained according to these attached drawings.
Fig. 1 is mycelia of the horizontal mould Lichtheimia ramosa 7C2 of stalk of branch provided by the invention under 40 times of object lens
Volume morphing.
Specific embodiment
It, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal condition or manufacturer
It is recommended that condition carry out.Reagents or instruments used without specified manufacturer is the routine that can be obtained by commercially available purchase
Product.
Stalk horizontal to the branch of the embodiment of the present invention is mould below is specifically described.
A kind of horizontal stalk of branch of offer of the embodiment of the present invention is mould, is specially the horizontal mould Lichtheimia ramosa of stalk of branch
7C2 bacterial strains, the bacterial strain on November 15th, 2017 in being preserved in the training of Hubei Wuhan Wuchang Luo Jia Shan Wuhan University Chinese Typical Representative
Object collection is supported, preserving number is：CCTCC NO：2017696.
Liquid nitrogen CTAB methods extraction genome can be used in the horizontal mould Lichtheimia ramosa 7C2 of stalk of branch of the present invention
DNA, using the mould 18S rDNA conserved sequences of the horizontal stalk of universal primer ITS1/ITS4 amplification branches, amplified fragments 750bp is adopted
With clone sequencing, with 3730 sequenators, the mould 18S rDNA conserved sequences of the horizontal stalk of the branch are obtained, provided by the invention point
The sequence of the mould 18S rDNA of the horizontal stalk of branch is as shown in SEQ ID No.1.In NCBI (National Center for
Biotechnology Information) comparison of US National Biotechnology Information center nucleic acid database, with
The horizontal mould uniformity of stalk of Lichtheimia ramosa branches reaches more than 99%.
Wherein, the sequence of primer is as follows：
The sequence of ITS1 is：5 '-TCCGTAGGTGAACCTGCGG-3 ' (as shown in SEQ ID No.2)；
The sequence of ITS4 is：5 '-TCCTCCGCTTATTGATATGC-3 ' (as shown in SEQ ID No.3).
The mould cultural characteristic of the horizontal stalk of branch is as follows：In dusty yeast peptone dextrose agar medium (YPD culture mediums：Ferment
Female powder 1%, peptone 2%, glucose 2%, agar 2%, pH6.5-7.0) surface seeding, 30 DEG C of culture 3d.Bacterium colony gas silk is rich
Richness, sprawling property is strong, covers 9cm diameter plates.Attached drawing 1 is refer to, celadon is presented in the middle part of white colony.
The mould cultural characteristic of the horizontal stalk of branch is as follows：Attached drawing 1 is refer to, in 40 times of object Microscopic observations, bacterium tool siphon
With circular sporangiospore.
The mould physio-biochemical characteristics of the horizontal stalk of branch are as follows：Culture display growth in sorghum rice culture medium test tube is vigorous,
Illustrate that there is production amylase ability.Enzyme is extracted using phosphate buffer, iodine solution is added in after enzyme solution hydrolysis soluble starch, makes indigo plant
Decoloration illustrates with alpha amylase and glucoamylase activity.
The mould biological characteristics of the horizontal stalk of branch is as follows：The horizontal growth temperature for obstructing mould (Lichtheimia ramosa 7C2) of branch
It spends for 20 DEG C~45 DEG C, optimum growth temperature is 30 DEG C~35 DEG C, can be grown in the range of pH4.0~7.0, sorghum rice
Ethanol-torlerance in culture medium is less than or equal to 6.2%.
The mould Lichtheimia ramosa 7C2 of the horizontal stalk of branch of the present invention in 30 DEG C of culture 7d of YPD Agr culture medium,
Continuous passage number is commissioned to train foster, and cultural characteristic and morphological feature have no significant change, production biacetyl level 30mg/L~
100mg/L.The biological character of the bacterial strain is basicly stable.
The present invention provides the horizontal stalk of branch the mould application in wine brewing, the mould production biacetyl energy of the horizontal stalk of branch provided by the invention
Power is stronger, and it is pure and sweet net refreshing to assign aromatic Chinese spirit, and plays the role of promoting fragrance, improves distillation yield and quality product rate.
Further, the horizontal stalk of branch provided by the invention is mould applies in wine brewing, including following either step：
(1) during wine brewing, the horizontal stalk of branch mould is mixed with liquor-making raw material；
(2) in distiller's yeast production process, the horizontal stalk of branch mould is mixed with starter-making materials；
(3) pit mud cultivation with it is aging during, the horizontal stalk of branch mould is mixed with pit mud.
Specifically, in distiller's yeast production process, by branch it is horizontal stalk it is mould mix with starter-making materials, reinforcing song by way of,
Base liquor ingredient is made to keep basicly stable, keeps the high production of biacetyl, improves distillation yield and quality product rate.
Pit mud is the basis of the good wine of Luzhou-flavor, and the quality of pit mud determines the quality of vinosity.Because pit mud is acetic acid bacteria, first
The carrier of the various beneficial objects such as alkane bacterium, butyric and habitat and procreation hotbed, these beneficial microorganism species and
Quantity be weigh pit mud quality a standard, add branch provided by the invention it is horizontal stalk it is mould, the production of biacetyl can be improved
Go out, then improve vinosity.
It is mould containing the horizontal stalk of above-mentioned branch the present invention also provides a kind of distiller's yeast.
The present invention provides the preparation method of above-mentioned distiller's yeast, including：It mixes the horizontal stalk of starter-making materials and above-mentioned branch is mould.
Above-mentioned starter-making materials are specially the raw material for making distiller's yeast, and starter-making materials can be appointing in wheat bran, barley, wheat and Cereals
The combination for one or more of anticipating.
The present invention provides application of the above-mentioned distiller's yeast in wine brewing, including following either step：
(1) during wine brewing, distiller's yeast is mixed with liquor-making raw material；
(2) pit mud cultivation with it is aging during, distiller's yeast is mixed with pit mud.
It is mould with being mixed for the raw material of wine brewing including the horizontal stalk of above-mentioned branch the present invention also provides a kind of brewing method
It closes and ferments.
The present invention also provides a kind of wine, are made by above-mentioned brewing method.
The feature and performance of the present invention are described in further detail with reference to embodiments.
First embodiment
The mould separation method of the horizontal stalk of branch provided in this embodiment.
The horizontal mould Lichtheimia ramosa 7C2 bacterial strains of stalk of branch of the present invention, it is big to be located away from Hubei Luzhou-flavor medium temperature
Song, separation method are as follows：
The accurate koji powder sample 10g that weighs puts into the 90mL sterile waters containing bead scattered, gradient dilution to 10-1~10-4, 3 gradients are actually used, 100mL are taken to be coated with various each 2 wares of culture medium flat plate, 30 DEG C of culture 5d, daily picking colony is extremely
YEPD (Yeast Extract Peptone Dextrose Medium, also referred to as yeast extract powder peptone dextrose culture-medium) tablet
Purifying obtains the horizontal mould Lichtheimia ramosa 7C2 bacterial strains of stalk of branch.The YEPD culture mediums form：Yeast extract 10g,
Peptone 20g, glucose 20g, agar powder 20g, water 1000mL, pH are natural.
Second embodiment
The present embodiment provides the mould distiller's yeasts of the horizontal stalk of branch provided comprising first embodiment.
The horizontal mould Lichtheimia ramosa 7C2 strains of stalk of branch are taken, aseptically, a little mycelium of picking/
Spore is transferred in sterile YPD (yeast extract peptone glucose agar medium) slant medium, and 48h is cultivated at 28 DEG C~32 DEG C
~52h is level-one test tube slant seed.
By rice 50% (mass percent), sorghum rice valve 30%, wheat valve 20% mix, impregnate for 24 hours, drain,
500mL triangles bottled 100g, 121 DEG C of sterilizing 30min are inoculated with primary inclined plane spore.48h~52h is cultivated at 28 DEG C~32 DEG C,
After mycelia is covered with, shaking triangular flask makes solid state rheology object depart from bottom of bottle, and triangular flask was fallen, and continues culture two days.50 DEG C~60
DEG C ventilation drying obtain secondary seed for 24 hours.
Distiller's yeast production provided in this embodiment comprising the horizontal mould Lichtheimia ramosa 7C2 of stalk of branch is using former
Material：Rice 50% (mass percent), sorghum rice valve 30%, wheat valve 20% mix, and above-mentioned raw materials are mixed, are impregnated for 24 hours,
It drains, after sterile wind is cooled to 28 DEG C~32 DEG C, inoculation secondary seed, moisturizing aerlbic culture 3 days or so in ventilation slot, until
Sporogenesis.50 DEG C of -60 DEG C of ventilation drying are used to produce for 24 hours afterwards.
It should be noted that in other embodiments, the starter-making materials that koji-making production uses can be wheat bran or other grains
Paddy, such as sweet potato.
The moisture of distiller's yeast (block is bent) provided in this embodiment can reach 8.7%, and saccharifying power can reach 2100 units
(according to People's Republic of China (PRC) light industry standard QB/T 4257-2011：Wine brewing yeast universaling analysis method).It needs to illustrate
, should use as early as possible after distiller's yeast qualification, prevent vigor from failing.
3rd embodiment
Verify the ability of the production biacetyl of the horizontal mould Lichtheimia ramosa 7C2 of stalk of branch provided by the invention.
1. experimental method
Five grains mix grain after moistening grain, and uploading in rice steamer hangs wine proportioning water.Grain grain after taking out from retort and cooling added in when lower bent 20% yeast and
The distiller's yeast that 1% second embodiment of yeast quantity provides, enters to store material temperature and does as usual, and the fermentation phase is not required to special maintenance.Act heap after storing
Grain mixes grain, and uploading in rice steamer hangs wine, leaves out the beginning and the end, and takes the wine heart (alcohol content 60%).Using gas chromatographic analysis, diacetyl content compared with
Compare pit (song, but the distiller's yeast of second embodiment offer are provided).
2. experimental result is as shown in table 1
Table 1 is the results of comparison of diacetyl content
(remarks：Processing pit is to add the pit for the distiller's yeast mould comprising horizontal stalk that second embodiment of the invention provides).
As shown in Table 1, the diacetyl content 1153.5mg/L of the wine heart of pit is handled, compares the biacetyl of the pit wine heart
Content 640.2mg/L adds double second of the pit for the distiller's yeast mould comprising horizontal stalk that can promote second embodiment of the invention offer
Acyl content adds 80%.
Described in increasing, a kind of horizontal stalk of branch of offer of the invention is mould, and preserving number is：CCTCC NO：2017696.This point
The mould diacetyl content for mix with the distiller's yeast of brewed wine, base liquor being improved of the horizontal stalk of branch, enhancement fragrance, with improve distillation yield with
Quality product rate.In addition, the present invention also provides the one kind its can brew pure and sweet net refreshing, dense aroma distiller's yeast and its application and
A kind of wine and its manufacturing method of pure and sweet net refreshing, dense aroma.
The foregoing is only a preferred embodiment of the present invention, is not intended to limit the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.Within the spirit and principles of the invention, that is made is any
Modification, equivalent substitution, improvement etc., should all be included in the protection scope of the present invention.
SEQUENCE LISTING
<110>Sky dragon Yellow Crane Tower the wine industry Xianning Co., Ltd
<120>A kind of horizontal stalk of branch is mould and its applies
<130> 250
<160> 3
<170> PatentIn version 3.5
<210> 1
<211> 785
<212> DNA
<213>The horizontal stalk of branch is mould
<400> 1
cgcgactagt tgatttcaga tctagatgtg agtttgttca agagaagacc caagttagat 60
cctctcacta gtccgtaaag attaagacct agttcaagaa gagactgatt gacttggaat 120
tatgaactca ctaaccaagt cgttgctctt caggcactct agagtctaca tccggcaaat 180
gactaaagcc aattgcctag gactaaatgt atttaaggcc atgacagcaa ctgaatgcca 240
tcaacacaag cccatttcca gctcgcttag gttcaaagaa ccaagttgaa ctgattggta 300
gttgcagata ctgaaacaac tgtgcctagt agattgacta ctaggcgcaa gatgcgttcg 360
agaactcgat gattcgctat gaatgcaagt cgcaataatt atcgcacttt gctacgctct 420
tcatcgatgc gagaaccaag agatccattg ccaagagttg tttttaagtt aacaactaac 480
tttttcgtac atcatggttt acacgagaag aataaacacc tttggggata gttaatacta 540
gagccccaaa ggcttgcctt gcttgctttg acttcaaaga cgtaggtctc ctgaaagaag 600
ggtccataca tcttctcttt tcaagcagcc tagatcttag tagtggcaca agtctcctaa 660
aaggagggtc tctatgccaa caaccagatc tacagcacaa ggcagagcca accaagttga 720
cccgactttg cacaaaactg tgaggaacta ctagagggga agagacccca actagtggtt 780
taact 785
<210> 2
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<212> DNA
<213>Artificial sequence
<400> 2
tccgtaggtg aacctgcgg 19
<210> 3
<211> 20
<212> DNA
<213>Artificial sequence
<400> 3
tcctccgctt attgatatgc 20
Claims (10)
1. a kind of horizontal stalk of branch is mould, which is characterized in that its preserving number is：CCTCC NO：2017696.
2. the horizontal stalk of branch is mould according to claim 1, which is characterized in that the mould 18S rDNA sequences of the horizontal stalk of branch are such as
Shown in SEQ ID No.1.
3. the mould application in wine brewing of the horizontal stalk of branch described in claim 1 or 2.
4. application according to claim 3, which is characterized in that it includes following either step：
(1) during wine brewing, the horizontal stalk of the branch mould is mixed with liquor-making raw material；
(2) in distiller's yeast production process, the horizontal stalk of the branch mould is mixed with starter-making materials；
(3) pit mud cultivation with it is aging during, the horizontal stalk of the branch mould is mixed with pit mud.
5. a kind of distiller's yeast, which is characterized in that the horizontal stalk of branch that it contains described in claim 1 or 2 is mould.
6. the preparation method of distiller's yeast as claimed in claim 5, which is characterized in that it includes：By starter-making materials and claim 1
Or the horizontal mould mixing of stalk of branch described in 2.
7. the preparation method of distiller's yeast according to claim 6, which is characterized in that the starter-making materials are wheat bran, barley, small
The combination of any one or more in wheat and Cereals.
8. application of the distiller's yeast described in claim 5 in wine brewing, which is characterized in that it includes following either step：
(1) during wine brewing, the distiller's yeast is mixed with liquor-making raw material；
(2) pit mud cultivation with it is aging during, the distiller's yeast is mixed with pit mud.
9. a kind of brewing method, which is characterized in that it includes the horizontal stalk of the branch described in claim 1 or 2 is mould with being used to make wine
Raw material mixed and fermented.
10. a kind of wine, which is characterized in that it is made as the brewing method described in claim 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810063402.4A CN108118002B (en) | 2018-01-22 | 2018-01-22 | Acremonium ramosum and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810063402.4A CN108118002B (en) | 2018-01-22 | 2018-01-22 | Acremonium ramosum and application thereof |
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| Publication Number | Publication Date |
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| CN108118002A true CN108118002A (en) | 2018-06-05 |
| CN108118002B CN108118002B (en) | 2021-04-09 |
Family
ID=62234063
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| CN201810063402.4A Active CN108118002B (en) | 2018-01-22 | 2018-01-22 | Acremonium ramosum and application thereof |
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Cited By (6)
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| CN108707556A (en) * | 2018-06-11 | 2018-10-26 | 华南农业大学 | A kind of horizontal stalk fungal strain and its application in producing cellulase |
| CN109181948A (en) * | 2018-07-27 | 2019-01-11 | 江苏洋河酒厂股份有限公司 | A kind of preparation method of nucleosides function Xiaoqu wine |
| CN113444646A (en) * | 2021-06-02 | 2021-09-28 | 湖北稻花香酒业股份有限公司 | Mould for producing beta-caryophyllene and application thereof |
| CN113980817A (en) * | 2021-08-26 | 2022-01-28 | 轻工业环境保护研究所 | Acremonium ramosum and application thereof |
| CN115353982A (en) * | 2022-09-13 | 2022-11-18 | 劲牌有限公司 | High-yield phenolic acid-yield aureobasidium ramosum and application thereof in fen-flavor Xiaoqu liquor |
| CN117143744A (en) * | 2023-08-29 | 2023-12-01 | 四川轻化工大学 | Bremia mycorrhizae and application thereof in preparation of yellow water esterified liquid |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108707556A (en) * | 2018-06-11 | 2018-10-26 | 华南农业大学 | A kind of horizontal stalk fungal strain and its application in producing cellulase |
| CN108707556B (en) * | 2018-06-11 | 2019-10-18 | 华南农业大学 | A kind of horizontal stalk fungal strain and its application in production cellulase |
| CN109181948A (en) * | 2018-07-27 | 2019-01-11 | 江苏洋河酒厂股份有限公司 | A kind of preparation method of nucleosides function Xiaoqu wine |
| CN109181948B (en) * | 2018-07-27 | 2022-01-07 | 江苏洋河酒厂股份有限公司 | Preparation method of nucleoside functional Xiaoqu liquor |
| CN113444646A (en) * | 2021-06-02 | 2021-09-28 | 湖北稻花香酒业股份有限公司 | Mould for producing beta-caryophyllene and application thereof |
| CN113444646B (en) * | 2021-06-02 | 2022-09-02 | 湖北稻花香酒业股份有限公司 | Mould for producing beta-caryophyllene and application thereof |
| CN113980817A (en) * | 2021-08-26 | 2022-01-28 | 轻工业环境保护研究所 | Acremonium ramosum and application thereof |
| CN113980817B (en) * | 2021-08-26 | 2023-06-23 | 北京市科学技术研究院资源环境研究所 | Bremia mycorrhizae and application thereof |
| CN115353982A (en) * | 2022-09-13 | 2022-11-18 | 劲牌有限公司 | High-yield phenolic acid-yield aureobasidium ramosum and application thereof in fen-flavor Xiaoqu liquor |
| CN115353982B (en) * | 2022-09-13 | 2023-09-12 | 劲牌有限公司 | High-yield phenolic acid branched aureobasidium pullulans and application thereof in fen-flavor Xiaoqu liquor |
| CN117143744A (en) * | 2023-08-29 | 2023-12-01 | 四川轻化工大学 | Bremia mycorrhizae and application thereof in preparation of yellow water esterified liquid |
| CN117143744B (en) * | 2023-08-29 | 2024-03-26 | 四川轻化工大学 | Bremia mycorrhizae and application thereof in preparation of yellow water esterified liquid |
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