CN108102389A - A kind of preparation method of clean type sterilization gelatin film - Google Patents
A kind of preparation method of clean type sterilization gelatin film Download PDFInfo
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- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
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Abstract
The present invention relates to gelatin film preparing technical fields, and in particular to a kind of preparation method of clean type sterilization gelatin film.The fruit of summer cypress is obtained liquid powder by the present invention by processing,Grass carp dry fish scale is taken to obtain enzymolysis fish scale by processing,Enzymolysis fish scale is obtained into gelatin solution by processing,Using discarded shrimp shell as raw material,Chitosan powder is obtained by processing,Prepare chitosan acid solution,By surface-active compound,Gelatin solution,Liquid powder,Solid hydrogen peroxide,The material mixings such as tea polyphenols obtain mixed glue solution,Mixed glue solution is finally poured into tape casting in tape casting machine,Re-dry obtains clean type sterilization gelatin film,Alkylamide propyl dimethyl amine glycine betaine is attached to micelle surface and tears the oiliness film layer of grease,So as to reach apparent cleaning action,Unrighted acid there are many also containing in gelatin solution,The Oxidation of Fat and Oils that can inhibit in food is rotten and mouldy,So that clean type sterilization gelatin film achievees the purpose that sterilization and toxic side effect is small,It has a extensive future.
Description
Technical field
The present invention relates to gelatin film preparing technical fields, and in particular to a kind of preparation method of clean type sterilization gelatin film.
Background technology
Extract of the gelatin as animal collagen tissue is a kind of natural polymer and biological macromolecule material.It
With good biocompatibility, no antigen, hemostasis and promote the performances such as organization healing, therefore, gelatin is also widely used
Into medicine and chemical industry.The gelatin resources reserve in China is enriched, derived from a wealth of sources, is cheap, and since gelatin is good
The performances such as reactivity, film forming, coagulability, biocompatibility, degradability, green environmental protection and recyclability make it near
Become the research hotspot of biological macromolecule material scientific domain over year.But because its thermal stability is low, bad mechanical property, meet it is water-soluble
It is swollen, be easy to degrade, dry after the features such as quality is crisp, forming ability is not strong and resistance is poor limit its application, it is therefore often right
Gelatin is modified processing.
With the development of economy, development of the packaging industry in China goes from strength to strength, they bring just not just for people
Profit and benefit, due to the nondegradable characteristic of these packaging materials, discarded object gradually causes human environment more and more
Harm.Film has edibility and degradability made of gelatin, has in terms of food and Key works Drug packing potentially using valency
Value.The spies such as good film forming ability that gelatin film has, low water vapo(u)r transmission, good oxygen barrier, natural inoxidizability
Point is expected to become a kind of new, environmentally friendly packaging material.
Then the preparation method of domestic and international gelatin film takes drying to take off film mainly using will be directly poured on into film liquid on substrate
Mode, this coating method is although easy to operate, but is not easy that continuous batch is film-made and the film for preparing is less uniform.Separately
Outside, since the thermal stability of gelatin film is low, mechanical strength is not high and preparation process is immature etc., so as to seriously constrain gelatin
The development and application of film.
It would therefore be highly desirable to developing a kind of gelatin film that can solve above-mentioned performance issue is highly desirable.
The content of the invention
Present invention mainly solves the technical issues of, for current gelatin film for oily food pack when, greasy dirt easily oozes
Go out, and oil substances in air can oxidation deterioration, it is easily mouldy after a period of time the defects of, provide a kind of clean type and kill
The preparation method of bacterium gelatin film.
In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is：
A kind of preparation method of clean type sterilization gelatin film, it is characterised in that specifically preparation process is：
（1）100~120g fruit of summer cypress is taken, is placed in conical flask, 300~350mL deionized waters are added in into conical flask, is impregnated,
Maceration extract is obtained, maceration extract is placed in ultrasonic cleaner, starts washer, cleaning cleans, obtains fruit of summer cypress medicine repeatedly
Liquid is filtered fruit of summer cypress liquid with non-woven fabrics, removes filter residue, obtains filtrate, filtrate is filtered, and removes residue, receives
Collection obtains extracting solution, extracting solution is transferred in evaporating dish, heat temperature raising, evaporation drying, obtains liquid powder；
（2）40~50g grass carps dry fish scale is taken to add in the beaker equipped with 400~450mL aseptic deionized waters, beaker water-bath is added
Heat, heating, then 4~5g trypsase is added in beaker, heat preservation enzymolysis, filtering removal filtrate obtains enzymolysis fish scale, will digest
Fish scale is washed with deionized, then the enzymolysis fish scale after washing is mixed with deionized water, heating water bath heating, enzyme deactivation, cooling,
Heat preservation concentration, obtains gelatin solution；
（3）Discarded shrimp shell is eluted with water, exposure drying obtains the shrimp shell of clean dried, by 100~120g clean drieds
Shrimp shell, which is placed in 200~230mL dilute hydrochloric acid, impregnates, and shrimp shell is taken out after immersion, is washed with distilled water shrimp shell to cleaning solution and is in
Neutrality obtains the shrimp shell of acidleach processing, and the shrimp shell that acidleach is handled is placed in 120~150mL sodium hydroxide solutions, and heating rises
Temperature, after boiling, filtering removal filtrate obtains gel, gel is placed in baking oven, heat temperature raising, chitosan powder is obtained after dry
End；
（4）50~55g Chitosan powders are placed in beaker, acetum is added in into beaker, adjust pH, obtain chitosan acid
Liquid mixes alkylamide propyl dimethyl amine alkali solution of beet with chitosan acid solution in equal volume, be placed in supercentrifuge from
The heart removes upper liquid, obtains sediment, put the precipitate in baking oven, and heat temperature raising is dry, obtains surface-active compound；
（5）Count in parts by weight, by 20~30 parts of surface-active compounds, 50~60 parts of gelatin solutions, 20~25 parts of liquid powder,
15~20 parts of Solid hydrogen peroxides, 100~120 parts of deionized waters, 10~15 parts of tea polyphenols mixing, are placed in high speed dispersor high speed
It is scattered, obtain mixed glue solution；
（6）Mixed glue solution is poured into tape casting machine and is cast, after glue curtain coating to be mixed, standing and drying obtains curtain coating glue
Glued membrane is placed in baking oven by film, heat temperature raising, dry, obtains clean type sterilization gelatin film.
Step（1）The dip time for 22~for 24 hours, it is 15 that maceration extract, which is placed in the time cleaned in ultrasonic cleaner,
~20min, temperature is 45~50 DEG C after heat temperature raising after extracting solution is transferred in evaporating dish, and the evaporation drying time is 2~3h.
Step（2）Temperature is 35~40 DEG C after the beaker water-bath heat temperature raising, and heat preservation enzymolysis time is 4~5h, will
Enzymolysis fish scale and deionized water mixing quality ratio after washing are 1 ︰ 10, and temperature is 90~95 DEG C after heating water bath heating, enzyme deactivation
Time is 10~15min, and temperature is 55~60 DEG C after cooling, and heat preservation concentration time is 6~7h.
Step（3）The exposure drying time is 2~3 days, and the mass fraction of dilute hydrochloric acid is 5%, soaking time for 2~
3h, the mass fraction of sodium hydroxide solution is 55%, and temperature is 100~110 DEG C after heat temperature raising, and digestion time is 4~5h, is added
Temperature is 80~90 DEG C after heat heating, and drying time is 4~6h.
Step（4）The mass fraction of the acetum is 2%, and it is 5.2~5.6 to adjust pH, alkylamide propyl diformazan
The mass fraction of base amine alkali solution of beet is 20%, and supercentrifuge rotating speed is 4000~4200r/min, centrifugation time for 15~
20min, temperature is 100~120 DEG C after heat temperature raising, and drying time is 2~3h.
Step（5）The high speed dispersor rotating speed is 3000~3300r/min, and the high speed dispersion time is 13~15min.
Step（6）Described controls scraper height as 0.5~2.0mm, and curtain coating base band rate travel is 0.5cm/s~1.5cm/s, is stood
Drying time is 2~3h, is 70~80 DEG C to temperature after baking oven heat temperature raising, drying time is 8~10h.
The beneficial effects of the invention are as follows：
（1）The present invention is impregnated in the fruit of summer cypress in deionized water, obtains maceration extract, by ultrasonic cleaning, filtering, then filter,
Evaporation drying obtains liquid powder, and grass carp dry fish scale is taken to add in aseptic deionized water, with trypsin digestion, refilters to obtain
Fish scale is digested, enzymolysis fish scale is mixed with deionized water, enzyme deactivation cools down again under the high temperature conditions is concentrated to give gelatin solution, with discarded
Shrimp shell for raw material, obtain gel by acidleach, soda boiling, then gel drying is obtained into Chitosan powder, prepare chitosan acid solution,
Alkylamide propyl dimethyl amine alkali solution of beet with chitosan acid solution is mixed, is lived by centrifuging, being dried to obtain surface
Property compound, by material mixings such as surface-active compound, gelatin solution, liquid powder, Solid hydrogen peroxide, tea polyphenols, is placed in height
Fast dispersion machine high speed disperses to obtain mixed glue solution, and mixed glue solution finally is poured into tape casting in tape casting machine, re-dry
Clean type sterilization gelatin film is obtained, the clean type sterilization gelatin film prepared by the present invention can absorb the moisture in air, redisperse
Colloid is formed, alkylamide propyl dimethyl amine glycine betaine is attached to micelle surface by grease as a kind of surface reactive material
Oiliness film layer tear, clean type sterilization gelatin film neutral and alkali ingredient weakly acidic grease is dissociated, be adsorbed in gelatin formation
In gel, so as to reach apparent cleaning action；
（2）Clean type sterilization gelatin film prepared by the present invention is with antioxidant such as the liquid powder of the fruit of summer cypress, tea polyphenols
Reach fungistatic effect, containing abundant triterpenoid saponin and flavone compound wherein in the fruit of summer cypress, to Escherichia coli and golden yellow
Color staphylococcus etc. has stronger inhibitory action, is the substance of a kind of natural degradable, fruit of summer cypress toxicity is low, in solid dioxygen
Water outlet and oxygen are decomposed in water oxygen sterilization process, thus is generated without the secondary substance of any poison, will not be produced when food packaging is applied
Raw toxic side effect, is a kind of natural animal-plant fiber with the made Chitosan powder of shrimp shell, to the antibacterial action of aquatic pathogen
Very by force and with stronger degradability, when cleaning oily food, chitin fiber gathers clean type sterilization gelatin film in shrimp shell shell
In the micelle of Icing Sugar entanglement effect it is extremely strong, the bacterium that grease exposes after being cleaned can by all kinds of antibacterial substance killings in gelatin,
In addition also contain there are many unrighted acid in the made gelatin solution of fish scale, the Oxidation of Fat and Oils that can inhibit in food goes bad and sends out
It is mould, so that clean type sterilization gelatin film achievees the purpose that sterilization and toxic side effect is small, have a extensive future.
Specific embodiment
100~120g fruit of summer cypress is taken, is placed in conical flask, 300~350mL deionized waters, dipping are added in into conical flask
22~for 24 hours, maceration extract is obtained, maceration extract is placed in ultrasonic cleaner, starts washer, cleans 15~20min, repeatedly clearly
It washes 2~4 times, obtains fruit of summer cypress liquid, fruit of summer cypress liquid is filtered with non-woven fabrics, remove filter residue, filtrate is obtained, to filtrate
It is filtered, removes residue, collection obtains extracting solution, extracting solution is transferred in evaporating dish, is heated to 45~50 DEG C, steams
Dry dry 2~3h, obtains liquid powder；40~50g grass carps dry fish scale is taken to add in equipped with 400~450mL aseptic deionized waters
In beaker, by beaker heating water bath, 35~40 DEG C are warming up to, then 4~5g trypsase is added in beaker, heat preservation enzymolysis 4~
5h, filtering removal filtrate, obtains enzymolysis fish scale, and enzymolysis fish scale is washed with deionized 3~5 times, then by the enzymolysis after washing
Fish scale is mixed with deionized water for 1 ︰ 10 in mass ratio, and heating water bath is warming up to 90~95 DEG C, and 10~15min of enzyme deactivation is cooled to
55~60 DEG C, heat preservation 6~7h of concentration obtains gelatin solution；Discarded shrimp shell is eluted with water, exposure is dried 2~3 days, is obtained clean
The shrimp shell of 100~120g clean drieds is placed in 200~230mL mass fractions to impregnate 2 in 5% dilute hydrochloric acid by dry shrimp shell only
~3h takes out shrimp shell after immersion, is washed with distilled water shrimp shell to cleaning solution and is in neutrality, and obtains the shrimp shell of acidleach processing, will
The shrimp shell of acidleach processing is placed in 120~150mL mass fractions in 55% sodium hydroxide solution, to be heated to 100~110 DEG C,
After 4~5h of boiling, filtering removal filtrate obtains gel, and gel is placed in baking oven, is heated to 80~90 DEG C, dry 4~
Chitosan powder is obtained after 6h；50~55g Chitosan powders are placed in beaker, the acetic acid that mass fraction is 2% is added in into beaker
Solution adjusts pH to 5.2~5.6, obtains chitosan acid solution, by the alkylamide propyl dimethyl amine sweet tea that mass fraction is 20%
Dish aqueous slkali mixes in equal volume with chitosan acid solution, is placed in supercentrifuge and centrifuges 15 with the rotating speed of 4000~4200r/min
~20min removes upper liquid, obtains sediment, put the precipitate in baking oven, is heated to 100~120 DEG C, dry 2~
3h obtains surface-active compound；It counts in parts by weight, by 20~30 parts of surface-active compounds, 50~60 parts of gelatin solutions, 20
~25 parts of liquid powder, 15~20 parts of Solid hydrogen peroxides, 100~120 parts of deionized waters, 10~15 parts of tea polyphenols mixing, are placed in
With rotating speed 13~15min of high speed dispersion of 3000~3300r/min in high speed dispersor, mixed glue solution is obtained；By mixed glue solution
Pour into tape casting machine and be cast, control scraper height as 0.5~2.0mm, curtain coating base band rate travel for 0.5cm/s~
1.5cm/s, after glue to be mixed is cast, 2~3h of standing and drying obtains curtain coating glued membrane, and glued membrane is placed in baking oven, heats
70~80 DEG C, dry 8~10h are warming up to, obtains clean type sterilization gelatin film.
Example 1
The 100g fruit of summer cypress is taken, is placed in conical flask, 300mL deionized waters are added in into conical flask, 22h is impregnated, obtains maceration extract,
Maceration extract is placed in ultrasonic cleaner, starts washer, cleans 15min, cleans 2 times repeatedly, obtains fruit of summer cypress liquid, is used
Non-woven fabrics is filtered fruit of summer cypress liquid, removes filter residue, obtains filtrate, filtrate is filtered, and removes residue, and collection obtains
Extracting solution is transferred in evaporating dish by extracting solution, is heated to 45 DEG C, and evaporation drying 2h obtains liquid powder；Take 40g careless
Dried fish fish scale is added in the beaker equipped with 400mL aseptic deionized waters, by beaker heating water bath, is warming up to 35 DEG C, then by 4g pancreases
Protease is added in beaker, heat preservation enzymolysis 4h, and filtering removal filtrate obtains enzymolysis fish scale, enzymolysis fish scale is washed with deionized water
It washs 3~5 times, then the enzymolysis fish scale after washing is mixed with deionized water for 1 ︰ 10 in mass ratio, heating water bath is warming up to 90 DEG C,
Enzyme deactivation 10min, is cooled to 55 DEG C, and heat preservation concentration 6h obtains gelatin solution；Discarded shrimp shell is eluted with water, exposure is dried 2 days,
The shrimp shell of clean dried is obtained, the shrimp shell of 100g clean drieds is placed in 200mL mass fractions to impregnate 2h in 5% dilute hydrochloric acid, is soaked
Shrimp shell is taken out after bubble, shrimp shell to cleaning solution is washed with distilled water and is in neutrality, the shrimp shell of acidleach processing is obtained, acidleach is handled
Shrimp shell be placed in 120mL mass fractions in 55% sodium hydroxide solution, to be heated to 100 DEG C, after boiling 4h, filtering removal
Filtrate obtains gel, and gel is placed in baking oven, is heated to 80 DEG C, Chitosan powder is obtained after dry 4h；50g shells are gathered
Icing Sugar end is placed in beaker, and the acetum that mass fraction is 2% is added in into beaker, pH to 5.2 is adjusted, obtains chitosan acid solution,
The alkylamide propyl dimethyl amine alkali solution of beet that mass fraction is 20% with chitosan acid solution is mixed in equal volume, is placed in height
15min is centrifuged with the rotating speed of 4000r/min in fast centrifuge, upper liquid is removed, obtains sediment, put the precipitate in baking oven
In, 100 DEG C are heated to, dry 2h obtains surface-active compound；It counts in parts by weight, 20 parts of surface-actives is compounded
Object, 50 parts of gelatin solutions, 20 parts of liquid powder, 15 parts of Solid hydrogen peroxides, 100 parts of deionized waters, 10 parts of tea polyphenols mixing, are placed in height
With the rotating speed high speed dispersion 13min of 3000r/min in fast dispersion machine, mixed glue solution is obtained；Mixed glue solution is poured into tape casting
It being cast in machine, controls scraper height as 0.5mm, curtain coating base band rate travel is 0.5cm/s, after glue curtain coating to be mixed,
Standing and drying 2h obtains curtain coating glued membrane, and glued membrane is placed in baking oven, is heated to 70 DEG C, and dry 8h obtains clean type sterilization
Gelatin film.
Example 2
The 115g fruit of summer cypress is taken, is placed in conical flask, 325mL deionized waters are added in into conical flask, 23h is impregnated, obtains maceration extract,
Maceration extract is placed in ultrasonic cleaner, starts washer, cleans 18min, cleans 3 times repeatedly, obtains fruit of summer cypress liquid, is used
Non-woven fabrics is filtered fruit of summer cypress liquid, removes filter residue, obtains filtrate, filtrate is filtered, and removes residue, and collection obtains
Extracting solution is transferred in evaporating dish by extracting solution, is heated to 48 DEG C, and evaporation drying 2.5h obtains liquid powder；Take 45g
Grass carp dry fish scale is added in the beaker equipped with 425mL aseptic deionized waters, by beaker heating water bath, is warming up to 38 DEG C, then will
4.5g trypsase is added in beaker, heat preservation enzymolysis 4.5h, and filtering removal filtrate obtains enzymolysis fish scale, enzymolysis fish scale is spent
Ion water washing 4 times, then the enzymolysis fish scale after washing is mixed with deionized water for 1 ︰ 10 in mass ratio, heating water bath is warming up to
93 DEG C, enzyme deactivation 12min, 53 DEG C are cooled to, heat preservation concentration 6.5h obtains gelatin solution；Discarded shrimp shell is eluted with water, exposure is done
Dry 2 days, the shrimp shell of clean dried is obtained, the shrimp shell of 110g clean drieds is placed in 220mL mass fractions to be soaked in 5% dilute hydrochloric acid
2.5h is steeped, shrimp shell is taken out after immersion, is washed with distilled water shrimp shell to cleaning solution and is in neutrality, obtains the shrimp shell of acidleach processing,
The shrimp shell that acidleach is handled is placed in 130mL mass fractions in 55% sodium hydroxide solution, to be heated to 105 DEG C, boiling 4.5h
Afterwards, filtering removal filtrate obtains gel, and gel is placed in baking oven, is heated to 85 DEG C, chitosan powder is obtained after dry 5h
End；53g Chitosan powders are placed in beaker, the acetum that mass fraction is 2% is added in into beaker, pH to 5.4 is adjusted, obtains
It is the bodies such as 20% alkylamide propyl dimethyl amine alkali solution of beet and chitosan acid solution by mass fraction to chitosan acid solution
Product mixing, is placed in supercentrifuge and centrifuges 17min with the rotating speed of 4100r/min, removes upper liquid, obtains sediment, will be heavy
Starch is placed in baking oven, is heated to 110 DEG C, and dry 2.5h obtains surface-active compound；It counts in parts by weight, by 25
Part surface-active compound, 55 parts of gelatin solutions, 23 parts of liquid powder, 18 parts of Solid hydrogen peroxides, 110 parts of deionized waters, 14 parts of tea
Polyphenol mixes, and is placed in high speed dispersor with the rotating speed high speed dispersion 14min of 3200r/min, obtains mixed glue solution；By epoxy glue
Liquid is poured into tape casting machine and is cast, and controls scraper height as 1.2mm, is cast base band rate travel as 1.0cm/s, glue to be mixed
After liquid stream is prolonged, standing and drying 2.5h obtains curtain coating glued membrane, and glued membrane is placed in baking oven, is heated to 75 DEG C, dry 9h,
Obtain clean type sterilization gelatin film.
Example 3
The 120g fruit of summer cypress is taken, is placed in conical flask, 350mL deionized waters are added in into conical flask, dipping for 24 hours, obtains maceration extract,
Maceration extract is placed in ultrasonic cleaner, starts washer, cleans 20min, cleans 4 times repeatedly, obtains fruit of summer cypress liquid, is used
Non-woven fabrics is filtered fruit of summer cypress liquid, removes filter residue, obtains filtrate, filtrate is filtered, and removes residue, and collection obtains
Extracting solution is transferred in evaporating dish by extracting solution, is heated to 50 DEG C, and evaporation drying 3h obtains liquid powder；Take 50g careless
Dried fish fish scale is added in the beaker equipped with 450mL aseptic deionized waters, by beaker heating water bath, is warming up to 40 DEG C, then by 5g pancreases
Protease is added in beaker, heat preservation enzymolysis 5h, and filtering removal filtrate obtains enzymolysis fish scale, enzymolysis fish scale is washed with deionized water
It washs 5 times, then the enzymolysis fish scale after washing is mixed with deionized water for 1 ︰ 10 in mass ratio, heating water bath is warming up to 95 DEG C, goes out
Enzyme 15min, is cooled to 60 DEG C, and heat preservation concentration 7h obtains gelatin solution；Discarded shrimp shell is eluted with water, exposure is dried 3 days, is obtained
To the shrimp shell of clean dried, the shrimp shell of 120g clean drieds is placed in 230mL mass fractions to impregnate 3h in 5% dilute hydrochloric acid, is impregnated
After take out shrimp shell, be washed with distilled water shrimp shell to cleaning solution and be in neutrality, obtain acidleach processing shrimp shell, by acidleach processing
Shrimp shell is placed in 150mL mass fractions in 55% sodium hydroxide solution, to be heated to 110 DEG C, after boiling 5h, filtering removal filter
Liquid obtains gel, and gel is placed in baking oven, is heated to 90 DEG C, Chitosan powder is obtained after dry 6h；By 55g chitosans
Powder is placed in beaker, and the acetum that mass fraction is 2% is added in into beaker, pH to 5.6 is adjusted, obtains chitosan acid solution, will
The alkylamide propyl dimethyl amine alkali solution of beet that mass fraction is 20% mixes in equal volume with chitosan acid solution, is placed at a high speed
20min is centrifuged with the rotating speed of 4200r/min in centrifuge, upper liquid is removed, obtains sediment, put the precipitate in baking oven,
120 DEG C are heated to, dry 3h obtains surface-active compound；Count in parts by weight, by 30 parts of surface-active compounds,
60 parts of gelatin solutions, 25 parts of liquid powder, 20 parts of Solid hydrogen peroxides, 120 parts of deionized waters, 15 parts of tea polyphenols mixing, are placed at a high speed
With the rotating speed high speed dispersion 15min of 3300r/min in dispersion machine, mixed glue solution is obtained；Mixed glue solution is poured into tape casting machine
Middle curtain coating controls scraper height as 2.0mm, and curtain coating base band rate travel is 1.5cm/s, quiet after glue curtain coating to be mixed
It puts dry 3h and obtains curtain coating glued membrane, glued membrane is placed in baking oven, be heated to 80 DEG C, it is bright to obtain clean type sterilization by dry 10h
Glued membrane.
Comparative example
With the gelatin film of company of Jinan City production as a comparison case
The gelatin film in gelatin film and comparative example is sterilized to clean type produced by the present invention to be detected, testing result such as 1 institute of table
Show：
1st, Mechanics Performance Testing
Sample is cut into the sample that size is 180mm × 25mm according to ASTM D882-01, is placed on 25 DEG C, relative humidity
In 52% drier（Saturation Mg (NO3)2·6H2O solution）It is tested after balance at least 48.Test fixture spacing is 100mm, is stretched
Rate 10mm/min, test carry out at room temperature.To reduce influence of the environment epidemic disaster to test, test is completed in 10min.
5~10 samples are tested, take arithmetic mean of instantaneous value.
2nd, swelling behavior is tested
Sample is cut into size as 2.5cm × 2.5cm, is weighed（m1）, it is placed on 3min in 20 DEG C of distilled water, taking-up filter paper
The water of remained on surface is blotted, is then weighed（m1）, the swelling ratio S of sample=（m2-m1）/m1.3~5 samples are tested, arithmetic is taken to put down
Average.
3rd, going mouldy property is tested
The food pack of same size, thickness is made in example 1~3 and comparative example prepared by the present invention, and is packed into city
Moon cake similar in one species obtained by field and quality, size, sealing.For the accuracy of guarantee test, experiment is divided into 4
Group, every group 5 parts.It is taken out after 30d, go mouldy situation of the observation moon cake at 30 days and 60 days.
1 performance measurement result of table
From the data in table 1, it can be seen that clean type sterilization gelatin film produced by the present invention has, mechanical property is good, biocidal property is strong, hence it is evident that excellent
In comparative example product.Therefore, there is wide prospect of the application.
Claims (7)
1. a kind of preparation method of clean type sterilization gelatin film, it is characterised in that specifically preparation process is：
（1）100~120g fruit of summer cypress is taken, is placed in conical flask, 300~350mL deionized waters are added in into conical flask, is impregnated,
Maceration extract is obtained, maceration extract is placed in ultrasonic cleaner, starts washer, cleaning cleans, obtains fruit of summer cypress medicine repeatedly
Liquid is filtered fruit of summer cypress liquid with non-woven fabrics, removes filter residue, obtains filtrate, filtrate is filtered, and removes residue, receives
Collection obtains extracting solution, extracting solution is transferred in evaporating dish, heat temperature raising, evaporation drying, obtains liquid powder；
（2）40~50g grass carps dry fish scale is taken to add in the beaker equipped with 400~450mL aseptic deionized waters, beaker water-bath is added
Heat, heating, then 4~5g trypsase is added in beaker, heat preservation enzymolysis, filtering removal filtrate obtains enzymolysis fish scale, will digest
Fish scale is washed with deionized, then the enzymolysis fish scale after washing is mixed with deionized water, heating water bath heating, enzyme deactivation, cooling,
Heat preservation concentration, obtains gelatin solution；
（3）Discarded shrimp shell is eluted with water, exposure drying obtains the shrimp shell of clean dried, by 100~120g clean drieds
Shrimp shell, which is placed in 200~230mL dilute hydrochloric acid, impregnates, and shrimp shell is taken out after immersion, is washed with distilled water shrimp shell to cleaning solution and is in
Neutrality obtains the shrimp shell of acidleach processing, and the shrimp shell that acidleach is handled is placed in 120~150mL sodium hydroxide solutions, and heating rises
Temperature, after boiling, filtering removal filtrate obtains gel, gel is placed in baking oven, heat temperature raising, chitosan powder is obtained after dry
End；
（4）50~55g Chitosan powders are placed in beaker, acetum is added in into beaker, adjust pH, obtain chitosan acid
Liquid mixes alkylamide propyl dimethyl amine alkali solution of beet with chitosan acid solution in equal volume, be placed in supercentrifuge from
The heart removes upper liquid, obtains sediment, put the precipitate in baking oven, and heat temperature raising is dry, obtains surface-active compound；
（5）Count in parts by weight, by 20~30 parts of surface-active compounds, 50~60 parts of gelatin solutions, 20~25 parts of liquid powder,
15~20 parts of Solid hydrogen peroxides, 100~120 parts of deionized waters, 10~15 parts of tea polyphenols mixing, are placed in high speed dispersor high speed
It is scattered, obtain mixed glue solution；
（6）Mixed glue solution is poured into tape casting machine and is cast, after glue curtain coating to be mixed, standing and drying obtains curtain coating glue
Glued membrane is placed in baking oven by film, heat temperature raising, dry, obtains clean type sterilization gelatin film.
2. a kind of preparation method of clean type sterilization gelatin film according to claim 1, it is characterised in that：Step（1）Institute
The dip time stated for 22~for 24 hours, it is 15~20min that maceration extract, which is placed in the time cleaned in ultrasonic cleaner,, extracting solution turns
Temperature is 45~50 DEG C after heat temperature raising after moving in evaporating dish, and the evaporation drying time is 2~3h.
3. a kind of preparation method of clean type sterilization gelatin film according to claim 1, it is characterised in that：Step（2）Institute
Temperature is 35~40 DEG C after the beaker water-bath heat temperature raising stated, and heat preservation enzymolysis time is 4~5h, by the enzymolysis fish scale after washing and
Deionized water mixing quality ratio is 1 ︰ 10, and temperature is 90~95 DEG C after heating water bath heating, and the enzyme deactivation time is 10~15min, is dropped
Temperature is 55~60 DEG C after temperature, and heat preservation concentration time is 6~7h.
4. a kind of preparation method of clean type sterilization gelatin film according to claim 1, it is characterised in that：Step（3）Institute
The exposure drying time stated is 2~3 days, and the mass fraction of dilute hydrochloric acid is 5%, and soaking time is 2~3h, sodium hydroxide solution
Mass fraction is 55%, and temperature is 100~110 DEG C after heat temperature raising, and digestion time is 4~5h, after heat temperature raising temperature for 80~
90 DEG C, drying time is 4~6h.
A kind of 5. preparation method of clean type sterilization gelatin film according to claim 1, it is characterised in that step（4）It is described
's：The mass fraction of acetum is 2%, and it is 5.2~5.6 to adjust pH, the matter of alkylamide propyl dimethyl amine alkali solution of beet
It is 20% to measure fraction, and supercentrifuge rotating speed is 4000~4200r/min, and centrifugation time is 15~20min, temperature after heat temperature raising
It spends for 100~120 DEG C, drying time is 2~3h.
6. a kind of preparation method of clean type sterilization gelatin film according to claim 1, it is characterised in that：Step（5）Institute
The high speed dispersor rotating speed stated is 3000~3300r/min, and the high speed dispersion time is 13~15min.
7. a kind of preparation method of clean type sterilization gelatin film according to claim 1, it is characterised in that：Step（6）Institute
That states controls scraper height as 0.5~2.0mm, is cast base band rate travel as 0.5cm/s~1.5cm/s, the standing and drying time
It it is 70~80 DEG C to temperature after baking oven heat temperature raising, drying time is 8~10h for 2~3h.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112677583A (en) * | 2020-12-23 | 2021-04-20 | 青岛正大环保科技有限公司 | Heat-preservation and heat-insulation vacuum packaging bag and preparation method thereof |
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