CN108096620A - The purposes of tsaoko essential oil and its emulsion as air freshener and/or air bacteriostatic agent - Google Patents
The purposes of tsaoko essential oil and its emulsion as air freshener and/or air bacteriostatic agent Download PDFInfo
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Abstract
The present invention relates to the purposes of tsaoko essential oil and its emulsion as air freshener and/or air bacteriostatic agent.The tsaoko essential oil and tsaoko essential oil emulsion of the present invention have antibacterial and fungistatic effect for following noxious air microorganism：Escherichia coli (Escherichia coli), staphylococcus aureus (Staphylococcus aureus), Candida albicans (Candida albicans), Penicillium (Penicillium), the fungi of aspergillus (Aspergillus) and Cladosporium (Cladosporium).
Description
Technical field
The present invention relates to the use as air freshener and/or air bacteriostatic agent of tsaoko essential oil and its emulsion of plant source
On the way, can be widely used in room air pollution prevention.
Background technology
It is essentially all by chemistry such as ether, essence although there are many current market sales of air freshener species
Into being grouped into, these substances inherently air pollutants after chemical breakdown is carried out, and contain in air freshener
Chemical aroma agent can also generate harm to the nervous system of people, influence the growth and development of children.Many chemical synthesis preparations are made
Air freshener simply with fragrance temporarily cover foreign flavor indoors, can not really improve the quality of air, can not be to air
In microorganism play the role of dissipation.
Although indicating the effect of sterilization in many air fresheners packagings, can not actually kill bacterium in air and
Fungi, that is to say, that can not be used simultaneously as air freshener and air bacteriostatic agent.Therefore exploitation is highly desirable to human body
Safety air freshener, air bacteriostatic agent or have both it is with fresh air and sterilization, the preparation of fungistatic effect.
Natural plants are tellurian resource treasure-houses, and the mankind, which have natural plant object Quality Research thousands of years, to go through
History is the main source of drug.In recent years, with spies such as people are natural to plant source preparation, health, low toxicity, no chemical residues
The understanding of point, world market also increasingly increase the demand of natural health raw produce, and the development of plant extracts industry is more next
It is faster.Meanwhile the preparation of natural plant is typically the mixture of polytype ingredient, containing there are many chemical composition, is often contained
There are tens kinds to arrive hundreds of ingredient, microorganism is not likely to produce the resistance to the action of a drug.Therefore, in recent years, the research and development of plant source preparation and
Using progressively obtaining the attention of insider, and significant progress is obtained.
The exploitation of plant source preparation at present is there are mainly two types of mode, first, active ingredient is found from plant, as synthesis
The lead compound of the new chemicals of high-efficiency low-toxicity, second is that plant source preparation directly is made using plant resources.China's plant money
Source is very abundant, thus the exploitation of botanical components and preparation mostly in the second based on.Existing Activities of Some Plants source system at present
Agent product comes out, but wherein most is plant insecticide, and plant source sterilization/bacteriostatic agent is then less.
Tsaoko (Amomum tsaoko) is Zingiber (Zingiberaceae), the plant of Amomum (Amomum) perennial herb
Object is distributed mainly on the provinces and regions such as the Yunnan, Guangxi, Guizhou in China.Tsaoko has special strong pungent fragrance, mainly seasons
Fragrance is the good merchantable brand in cooking sauce.
The content of the invention
The present invention relates to the tsaoko essential oil from the extraction of the fruit of drying and ripening tsaoko and its emulsion as air freshener with/
Or the purposes of air bacteriostatic agent.
In a first aspect, the present invention provides purposes of the tsaoko essential oil as air freshener and/or air bacteriostatic agent, it is described
Tsaoko essential oil contains the characteristic component of following content by weight：
(1) 1,8- cineoles：45-65%；
(2) citral：1-3.5%.
Preferably, by weight, the content of 1,8- cineoles is 50-63%.Preferably, by weight, 1,8- folium eucalyptis
The content of oily element is 58-63%.
Second aspect, the present invention provides using tsaoko essential oil prepared by following preparation method as air freshener and/or
The purposes of air bacteriostatic agent, the described method includes：
(1) weigh a certain amount of dry tsaoko, knock fritter or be ground into coarse powder；
(2) to dry tsaoko fritter or coarse powder tsaoko essential oil is extracted as raw material；And
(3) tsaoko essential oil is collected with volatile oil extractor, and calculates oil yield.
The method of extraction tsaoko essential oil is selected from：Steam distillation, solvent extraction method, CO2Supercritical extraction technique, Asia are faced
Boundary's water extraction and microwave-assisted.
In the extracting method using steam distillation：
Dry tsaoko fritter or the weight ratio of coarse powder and water are 1:3-1:20, dry tsaoko fritter or coarse powder are soaked in water
When bubble 0-24 is small；Then when steam distillation extraction 0.5-10 is small；Tsaoko essential oil is collected with volatile oil extractor, and calculates oily
Rate.
Preferably, to dry the weight ratio of tsaoko fritter or coarse powder and water as 1:3-1:10, by dry tsaoko fritter or thick
Powder impregnate in water 0-4 it is small when；Then when steam distillation extraction 3-8 is small；Tsaoko essential oil is collected with volatile oil extractor, and is counted
Calculate oil yield.
Preferably, the weight ratio that can dry tsaoko fritter or coarse powder and water is 1:4, dry tsaoko fritter or coarse powder are existed
When immersion 1 is small in water；Then when steam distillation extraction 7 is small；Tsaoko essential oil is collected with volatile oil extractor, and calculates oily
Rate.
Dry tsaoko can be the drying tsaoko through drying acquisition or the drying tsaoko through drying acquisition.
The oil yield of the tsaoko essential oil of the present invention is by weight in the range of 1.0%-2.5%.The tsaoko essence of the present invention
Oil is pale yellow transparent grease, has special strong fragrance.
The tsaoko essential oil of the present invention as active ingredient, can be configured to for solid with fresh air, liquid, emulsion,
Spray and aerosol-type.In the situation of spray, propellant used is selected from dichlorodifluoromethan hydro carbons, hydrocarbon and compressed gas
Body.
The third aspect, the present invention provides purposes of the tsaoko essential oil emulsion as air freshener and/or air bacteriostatic agent,
The composition of the tsaoko essential oil emulsion by weight is as follows：
(1) 10 parts of tsaoko essential oil；
(2) solvent 20-35 parts；
(3) auxiliary agent 5-15 parts；With
(4) water 40-55 parts.
The solvent is selected from：Methanol, ethyl alcohol, n-butanol, normal propyl alcohol, isopropanol, isobutanol, n-amyl alcohol, isoamyl alcohol, just oneself
Alcohol, acetone, dichloromethane, ethyl acetate, dimethyl sulfoxide, tetrahydrofuran, dimethylformamide, cyclohexanone, pyrrolidones, N- first
The mixture of base pyrrolidones or wherein two or more.Preferably, the solvent is selected from：Ethyl alcohol, methanol, propyl alcohol, isopropyl
The mixture of alcohol, butanol, glycerine, acetone etc. or wherein two or more.Preferably, the solvent for methanol, ethyl alcohol or
The mixture of the two.
The auxiliary agent be surfactant, carrier and/or dispersant, the surfactant be selected from Tweens, spans,
Lauryl sodium sulfate, alcohols, propylene glycol, polyethylene glycol and glycerine, the dispersant be selected from calgon, sodium pyrophosphate,
Polyacrylic acid sodium salt and polyvinyl alcohol, the carrier are selected from liposome, cyclodextrin and micro-capsule.Preferably, the surfactant
Selected from Tweens, alkylphenol polyoxyethylene ether class, spans, lauryl sodium sulfate.Preferably, the surfactant is selected from
Tweens, alkylphenol polyoxyethylene ether class.
The emulsion of the tsaoko essential oil of the present invention is selected from：
By 1 part of tsaoko essential oil, 3.5 parts of ethyl alcohol, 1.5 parts of Tweens surfactant and 4 parts of breasts for stirring evenly acquisition of water
Agent；
By 1 part of tsaoko essential oil, 2 parts of ethyl alcohol, the 5.5 parts of stirrings of 1.5 parts of alkylphenol polyoxyethylene ether class surfactant and water
The emulsion uniformly obtained；
By 1 part of tsaoko essential oil, 3.5 parts of ethyl alcohol, the 4 parts of stirrings of 0.5 part of alkylphenol polyoxyethylene ether class surfactant and water
The emulsion uniformly obtained；
By 1 part of tsaoko essential oil, 3.5 parts of ethyl alcohol, the 4 parts of stirrings of 1.5 parts of alkylphenol polyoxyethylene ether class surfactant and water
The emulsion uniformly obtained；With
By 1 part of tsaoko essential oil, 2.5 parts of ethyl alcohol, 1 part of methanol, 1.5 parts of alkylphenol polyoxyethylene ether class surfactant and water
4 parts of emulsions for stirring evenly acquisition.
The tsaoko essential oil of the present invention as active ingredient, can be configured to for solid with fresh air, liquid, emulsion,
Spray and aerosol-type.
For example, the tsaoko essential oil of the present invention, which can be not pressurized to be directly added into aerosol can, is made spray or with propellant system
Into aerosol, carry out disinfection to room air sterilization.The situation of spray, propellant used are selected from dichlorodifluoromethan hydro carbons, hydrocarbon
Compound and compressed gas.The present invention tsaoko essential oil also can humidified device ultrasonic atomizatio, be disseminated in room air and disappear
Kill bacterium.These occupation modes are easy to operate, greatly simplifie production operation program, applied widely.It can also be according to need
Aromatic is added, is prepared into the product of various fragrance.
The tsaoko essential oil and tsaoko essential oil emulsion of the present invention has special, strong pungent fragrance, and mellowness is abnormal, ooze people
Heart spleen, therefore air freshener can be used as.
The tsaoko essential oil and tsaoko essential oil emulsion of the present invention has the antibacterial and suppression for following pathogenic air microbe
Bacterium effect：Escherichia coli (Escherichia coli), staphylococcus aureus (Staphylococcus aureus), white
Candida albicans (Candida albicans), aspergillus flavus (Aspergillus flavus), the dendritic mould (Cladosporium of branch spore
Cladosporioides), Polish mould (Penicillium polonicum) and penicillium chrysogenum (Penicillium
Chrysogenum), therefore, air bacteriostatic agent can be used as.
The tsaoko essential oil and tsaoko essential oil emulsion of the present invention has both the with fresh air and antibacterial double effects of air, comes from
It is safe and nontoxic, nonirritant in natural plants tsaoko, therefore be the excellent selection of air freshener and air bacteriostatic agent.
And the tsaoko essential oil emulsion of the present invention has compared to tsaoko essential oil antibacterial beyong contemplation itself, antibacterial effect
Fruit.
The tsaoko essential oil and tsaoko essential oil emulsion of the present invention is safe and non-toxic, nonirritant, can be not only used for the skies such as interior, warehouse
Between the dissipation of air and pure and fresh, it can also be used to the dissipation of air and pure and fresh in the vehicles such as train, automobile, aircraft.
Description of the drawings：
Fig. 1 illustrates the GC-MS chromatograms of the tsaoko essential oil of the present invention.
Fig. 2 illustrates inhibition of the tsaoko essential oil emulsion to aspergillus flavus.
Specific embodiment
Technical scheme is described further with reference to embodiment and Figure of description.These embodiments are only
For illustrating the present invention rather than limiting protection scope of the present invention.
Embodiment 1：The preparation of tsaoko essential oil
Each 0.5kg of the tsaoko for drying and drying is weighed respectively, is ground into coarse powder, impregnates 1h in 3.5L water.Vapor steams
Extraction 7h is evaporated, collects tsaoko essential oil with volatile oil extractor, calculating oil yield=(obtain oil mass/weigh tsaoko amount) × 100%, knot
Fruit is that dry the oil yield of tsaoko be 2.0%, and the oil yield for drying tsaoko is 1.3%.
Embodiment 2：The characterization of tsaoko essential oil
The constituent analysis of tsaoko essential oil
Using the ingredient and content of the tsaoko essential oil that the analysis of GC/MS methods is extracted by drying and drying tsaoko.By tsaoko essential oil
10000 times are diluted with n-hexane, 1 μ l of sample introduction.GC conditions：Using Thermo TR-5MS (30m*0.32mm, 0.25 μm) chromatography
Column, 50 DEG C of column initial temperature, 6 DEG C/min of temperature programming rise to 220 DEG C, then rise to 250 DEG C with 10 DEG C/min, constant temperature 5min.Sample introduction
250 DEG C, carrier gas He, carrier gas flux 2.0ml/min of temperature of mouth, split ratio 20:1.MS conditions：EI ion sources, scan pattern full
Scan, scanning range m/z 50-600amu, 250 DEG C of ion source temperature, 250 DEG C of GC/MS interface temperatures.
The GC-MS chromatograms of tsaoko essential oil are referring to attached drawing 1.The result shows that it is examined altogether in drying the tsaoko essential oil that tsaoko extracts
Go out chromatographic peak 29, wherein identifying 25 kinds of volatile matters, the total relative amount 98.7% of essential oil is accounted for, wherein the phase of 1,8- cineole
It is 58.11% to content, the relative amount of citral is 3.33%.First part of sample in the tsaoko essential oil of tsaoko extraction is dried to be total to
Chromatographic peak 33 is detected, wherein identifying 30 kinds of volatile matters, the total relative amount 97.75% of essential oil is accounted for, wherein 1,8- cineole
Relative amount for 62.73%, the relative amount of citral is 2.92%.Second part of tsaoko essential oil for drying tsaoko sample extraction
Chromatographic peak 32 is detected altogether, wherein identifying 30 kinds of volatile matters, the total relative amount 97.12% of essential oil is accounted for, wherein 1,8- eucalyptus oil
The relative amount of element is 50.29%, and the relative amount of citral is 3.34%.
Specific ingredient and content are referring to the following table 1：
Compared with table 1 dries the chemical composition of the tsaoko essential oil with drying tsaoko extraction：
As can be seen from the above table, in the three kinds of tsaoko essential oils obtained by three kinds of tsaoko samples, two kinds of main components and content
Composition is as follows：
1,8- cineoles：50-63%
Citral：2.9-3.4%.
The Odor Evaluations of tsaoko essential oil：Special, strong pungent fragrance, mellowness are abnormal, refreshing.
Embodiment 3：The preparation of tsaoko essential oil emulsion
1 part of tsaoko essential oil, 3.5 parts of ethyl alcohol, 1.5 parts of Tween 80,4 parts of water, stir evenly to get.
1 part of tsaoko essential oil, 2 parts of ethyl alcohol, 1.5 parts of alkylphenol polyoxyethylene ether (OP10), 5.5 parts of water stir evenly, i.e.,
.
1 part of tsaoko essential oil, 3.5 parts of ethyl alcohol, 0.5 part of alkylphenol polyoxyethylene ether (OP10), 4 parts of water stir evenly, i.e.,
.
1 part of tsaoko essential oil, 3.5 parts of ethyl alcohol, 1.5 parts of alkylphenol polyoxyethylene ether (OP10), 4 parts of water stir evenly, i.e.,
.
1 part of tsaoko essential oil, 2.5 parts of ethyl alcohol, 1 part of methanol, 1.5 parts of alkylphenol polyoxyethylene ether (OP10), 4 parts of water, stirring
Uniformly to get.
The Odor Evaluations of tsaoko essential oil emulsion：Special, strong pungent fragrance, mellowness are abnormal, refreshing.
Embodiment 4：Tsaoko essential oil inhibits air harmful fungoid with tsaoko essential oil emulsion to be tested
Experimental strain：Aspergillus flavus (Aspergillus flavus) and the dendritic mould (Cladosporium of branch spore
Cladosporioides) it is isolated from air-conditioning air.
Tsaoko essential oil emulsion：Using being formulated following emulsion in embodiment 3：
1 part of tsaoko essential oil, 3.5 parts of ethyl alcohol, 1.5 parts of alkylphenol polyoxyethylene ether (OP10), 4 parts of water stir evenly, i.e.,
.
Toxicity test is tested ----mycelial growth rate method
1) tsaoko essential oil is added to culture medium, mixing, cooling, similarly, by tsaoko according to 5000 times of extension rate
(concentration of tsaoko essential oil and tsaoko essential oil dilute 5000 times of phases to essential oil emulsion in itself wherein in tsaoko essential oil emulsion by 500 times
Extension rate together) is added to culture medium, mixing, cooling；
2) tsaoko essential oil is added to culture medium, mixing, cooling, similarly, by tsaoko according to 4000 times of extension rate
Essential oil emulsion is by 500 times (when the concentration of tsaoko essential oil dilutes 4000 times in itself less than tsaoko essential oil wherein in tsaoko essential oil emulsion
Concentration) extension rate be added to culture medium, mixing, cooling；
3) it is mould in the dendritic branch spore of above inoculation of medium 2) in above inoculation of medium aspergillus flavus 1), per plate
It is inoculated with 10 μ L spore suspensions, 6 repetitions, as test sample group；
3) tablet without medicament is inoculated with 3 strains respectively, and 10 μ L spore suspensions, 6 repetitions, as blank are inoculated with per plate
Control group；
4) in the culture 7 days of 25 DEG C of incubator.
Judge regulation：
Measure colony diameter (mm), calculating bacteriostasis rate, bacteriostasis rate=((control group colony diameter-test group colony diameter)/
Control group colony diameter) × 100%.
2 tsaoko essential oil of table and the comparison of tsaoko essential oil emulsion suppression air root-pruning effect
Can be seen that from above-mentioned 2 result of table when tsaoko essential oil dilutes 5000 times is 39.8% to the inhibiting rate of aspergillus flavus, is contained
The emulsion of tsaoko essential oil 10% reaches same concentrations when diluting 500 times, inhibiting rate is up to 72.2%, and fungistatic effect is better than tsaoko essence
Oil itself.Inhibiting rate is 70.19% when tsaoko essential oil dilutes 4000 times, and the emulsion of essential oil containing tsaoko 10% dilutes low at 500 times
In this concentration, and the inhibiting rate mould to dendritic branch spore is up to 100%, and fungistatic effect is also superior to tsaoko essential oil in itself.
Embodiment 5：Tsaoko essential oil emulsion is to the bacteriostasis of bacterium
According to country《Disinfection technology standard (version in 2002)》(Ministry of Health of the People's Republic of China, December 2002)
It is required that it is evaluated.
Experimental strain：
Escherichia coli (Escherichia coli) ATCC8099, staphylococcus aureus (Staphylococcus
Aureus) golden yellow subspecies ATCC6538 and Candida albicans (Candida albicans) ATCC10231 are bought from Beijing
Municipal Disease Control and Prevention Center, they are all bacteriums harmful in air.
Culture medium：
Nutrient broth medium is used for the culture of Escherichia coli and staphylococcus aureus；
Sabouraud culture medium is used for the culture of Candida albicans.
The formula of tsaoko essential oil emulsion is as follows：
1 part of tsaoko essential oil, 3.5 parts of ethyl alcohol, 1.5 parts of Tween 80,4 parts of water, stir evenly to get.
Minimal inhibitory concentration measures (nutrient broth dilution method) ----MIC (Minimal Inhibitory
Concentration)：
1) Escherichia coli, staphylococcus aureus and Candida albicans bacteria suspension are prepared；
2) tsaoko essential oil emulsion is done into two-fold dilution into various concentration by test solution with distilled water, respectively takes 2.5mL by test solution
It adds in the nutrient broth medium and Sharpe fluid nutrient medium test tube of the double concentration of 2.5mL.Concentration setting is as follows：Inhibit large intestine
The concentration gradient of bacillus is：0.05%th, 0.1%, 0.2%, 0.4%, 0.8%；Inhibit the concentration gradient of staphylococcus aureus
For：0.0625%th, 0.125%, 0.25%, 0.5%, 1%；Inhibit the concentration gradient of Candida albicans for 1%, 2%, 4%,
8%th, 16%；
3) nutrient broth medium of the double concentration of 2.5mL and Sharpe fluid nutrient medium test tube are added in 2.5mL distilled water
It is middle to be used as solvent control；
4) 0.1mL is taken to contain 108Escherichia coli, staphylococcus aureus and the Candida albicans bacteria suspension of cfu/mL be inoculated in
In upper test tube, as test group sample；
5) 0.1mL is taken to contain 108Escherichia coli, staphylococcus aureus and the Candida albicans bacteria suspension of cfu/mL is inoculated in not
The test tube of the essential oil containing tsaoko, as positive control；
6) 0.1mL is taken to contain 108Escherichia coli, staphylococcus aureus and the Candida albicans bacteria suspension of cfu/mL is inoculated in molten
Agent contrast test tube, as solvent control；
7) 2 blank cultures are taken as negative control；
8) sample sets of Escherichia coli and Staphylococcus aureus, control group are positioned over 37 DEG C of cultures, 24 it is small when after observe
As a result.The sample sets of Candida albicans, control group are positioned over 28 DEG C of cultures, 48 it is small when after observe result.
Judge regulation：
When positive control pipe has bacterial growth (muddiness), negative control pipe asepsis growth (transparent), test group asepsis growth
Highest dilution corresponding to anti-(suppression) bacteria suspension concentration, be the sample to the MIC of tested bacterium.
MIC result of the tests are as follows：Tsaoko essential oil is 0.2% to the MIC of Escherichia coli, to the MIC of staphylococcus aureus
For 0.125%, the MIC to Candida albicans is 4%.
Embodiment 6：Tsaoko essential oil emulsion is to the bacteriostasis of air root-pruning
Experimental strain：
Polish mould (Penicillium polonicum) is isolated from air-conditioning air.
Culture medium：Potato agar culture medium
The formula of tsaoko essential oil emulsion bacteriostatic agent is as follows：
1 part of tsaoko essential oil, 3.5 parts of ethyl alcohol, 1.5 parts of Tween 80,4 parts of water, stir evenly to get.
Toxicity test is tested ----EC50(half-maximal effect concentration)：
1) Polish mould bacteria suspension, bacterial concentration 10 are prepared8cfu/mL；
2) tsaoko essential oil emulsion is pressed to the concentration gradient of 500,200,100,50,25 μ L/L, added to PDA culture medium, is mixed
It is even, cooling；
3) each concentration is inoculated with Polish mould respectively, and 10 μ L, 6 repetitions, as test sample group are inoculated with per plate；
4) tablet without tsaoko essential oil formulation is inoculated with Polish mould respectively, and 10 μ L, 6 repetitions, as sky are inoculated with per plate
White control group；
5) in the culture 7 days of 25 DEG C of incubator.
Judge regulation：
Measure colony diameter (mm), calculating bacteriostasis rate, bacteriostasis rate=((control group colony diameter-test group colony diameter)/
Control group colony diameter) × 100%.Bacteriostasis rate is converted into niqueMin (Y), pharmacy quality concentration (μ L/L) is converted into numerical value
(X), virulence regression equation (Y=a+bX) and related coefficient (R) is obtained, calculates the drug concentration inhibited for examination growth 50%
(EC50)。
Tsaoko essential oil emulsion bacteriostatic agent is as shown in table 3 below to the antibacterial result of air root-pruning Poland mould.
3 tsaoko essential oil emulsion of table is to the fungistatic effect of Polish mould
Embodiment 7：Tsaoko essential oil emulsion is to the bacteriostasis of air root-pruning
Experimental strain：
Aspergillus flavus (Aspergillus flavus), dendritic branch spore mould (Cladosporium cladosporioides) and
Penicillium chrysogenum (Penicillium chrysogenum), is isolated from air-conditioning air.
Culture medium：Potato agar culture medium
The formula of tsaoko essential oil emulsion is as follows：
1 part of tsaoko essential oil, 3.5 parts of ethyl alcohol, 1.5 parts of alkylphenol polyoxyethylene ether (OP10), 4 parts of water stir evenly, i.e.,
.
Toxicity test is tested ----mycelial growth rate method
1) tsaoko essential oil emulsion is added to culture medium, mixing, cooling by the extension rate of 500 times, 1000 times；
2) each concentration is inoculated with 3 strains respectively, and 10 μ L, 6 repetitions, as test sample group are inoculated with per plate；
3) tablet without medicament is inoculated with 3 strains respectively, and 10 μ L, 6 repetitions, as blank control group are inoculated with per plate；
4) in the culture 7 days of 25 DEG C of incubator.
Judge regulation：
Measure colony diameter (mm), calculating bacteriostasis rate, bacteriostasis rate=((control group colony diameter-test group colony diameter)/
Control group colony diameter) × 100%.
The experimental results showed that (table 5), tsaoko essential oil emulsion is to air root-pruning aspergillus flavus (Aspergillus flavus), branch
Shape branch spore mould (Cladosporium cladosporioides), penicillium chrysogenum (Penicillium chrysogenum) have
Stronger inhibitory action.
5 tsaoko essential oil emulsion of table is to the fungistatic effect of air root-pruning
Embodiment 8：Tsaoko essential oil emulsion is to the Disinfection Effect of room air
According to national standard《Public place air hygiene method of inspection third portion：Air microbe》(GB/T
18204.3-2013) (National Standard of the People's Republic of China, in December, 201 4) is evaluated.
Culture medium：
Total number of bacteria is counted using nutrient agar；
Total number of fungi is counted using sabouraud's agar.
The formula of tsaoko essential oil emulsion is as follows：1 part of tsaoko essential oil, 3.5 parts of ethyl alcohol, alkylphenol polyoxyethylene ether (OP10)
1.5 parts, 4 parts of water, stir evenly to get.
Fungistatic effect evaluation-natural sedimentation
1) experiment is carried out in common lab, and area is about 15m2.In setting 3 on indoor cornerwise 3 Along ents
Sampled point, when sampling, close the doors and windows.Sampled point, apart from more than wall 1m, avoids air-conditioning, door and window apart from ground level 1.2-1.5m
Etc. at air circulations.Air sample before acquisition spray first, after the completion of acquisition, even spraying 25ml tsaoko essential oil emulsions, spraying
When nozzle spray to upper, middle and lower and surrounding, 2h, 4h monitor bacterium, fungi in air after spraying.
2) culture medium flat plate is placed at sampled point, opens ware lid, exposure 5min covers ware lid.Nutrient agar panel is put
In 36 DEG C of ± 1 DEG C of insulating boxs, 48h is cultivated, counts total number of bacteria；Sabouraud's agar tablet is put in 28 DEG C of insulating boxs, is cultivated 5 days, meter
Number total number of fungi.
3) clump count grown on every piece of tablet is counted, is denoted as every plate clump count (cfu/ wares).Whole sampled points are obtained
Average colony number, calculate bacteriostasis rate.Bacteriostasis rate=((average colony number after average colony number-spray before spray)/average colony before spraying
Number) × 100%.
The experimental results showed that (table 6), after tsaoko essential oil emulsion spraying 2h, total Bacteria Count of Bacteria in Indoor Air is reduced
75.0%, total number of fungi reduces 83.3%；Spraying 4h remains to reach 25.0% to the inhibiting rate of air-borne bacteria sum, true to air
The inhibiting rate of bacterium sum is 94.4%, shows that tsaoko essential oil emulsion can effectively inhibit the microorganism growth in room air.
6 tsaoko essential oil emulsion of table is to the Disinfection Effect of room air
Discussion of results：
As can be seen from the above embodiments with tsaoko essential oil tsaoko essential oil emulsion as active component compared with tsaoko essence
Oil has the effect of more significant inhibition air root-pruning in itself；Tsaoko essential oil emulsion also shows significantly to inhibit in air to human body
The effect of harmful bacterium.
Claims (11)
1. purposes of the tsaoko essential oil as air freshener and/or air bacteriostatic agent, the tsaoko essential oil contains by weight such as
The characteristic component of lower content：
(1) 1,8- cineoles：45-65%；
(2) citral：1-3.5%.
2. purposes according to claim 1, wherein the content of 1,8- cineoles is 50-63% by weight, it is preferable that with weight
The content of gauge 1,8- cineoles is 58-63%.
3. purposes of the tsaoko essential oil prepared in the following manner as air freshener and/or air bacteriostatic agent, the method bag
It includes：
(1) weigh a certain amount of dry tsaoko, knock fritter or be ground into coarse powder；
(2) to dry tsaoko fritter or coarse powder tsaoko essential oil is extracted as raw material；And
(3) tsaoko essential oil is collected with volatile oil extractor, and calculates oil yield；
The method for wherein extracting tsaoko essential oil is selected from：Steam distillation, solvent extraction method, CO2Supercritical extraction technique, Asia are faced
Boundary's water extraction and microwave-assisted.
4. purposes according to claim 3, wherein in the extracting method using steam distillation：
Dry tsaoko fritter or the weight ratio of coarse powder and water are 1:3-1:20, dry tsaoko fritter or coarse powder are impregnated into 0- in water
24 it is small when；Then when steam distillation extraction 0.5-10 is small；Tsaoko essential oil is collected with volatile oil extractor, and calculates oil yield,
The oil yield by weight in the range of 1.0%-2.5%,
Preferably, to dry the weight ratio of tsaoko fritter or coarse powder and water as 1:4-1:10, dry tsaoko fritter or coarse powder are existed
When immersion 0-4 is small in water；Then when steam distillation extraction 3-8 is small；Tsaoko essential oil is collected with volatile oil extractor, and is calculated
Oil cut rate；The dry tsaoko can be the drying tsaoko through drying acquisition or the drying tsaoko through drying acquisition,
Preferably, to dry the weight ratio of tsaoko fritter or coarse powder and water as 1:7, dry tsaoko fritter or coarse powder are soaked in water
Steep 1 it is small when；Then when steam distillation extraction 7 is small；Tsaoko essential oil is collected with volatile oil extractor, and calculates oil yield, it is described
Oil yield by weight in the range of 1.0%-2.5%, the dry tsaoko can be drying tsaoko through drying acquisition or
Person is the drying tsaoko through drying acquisition.
5. according to the purposes of any one of Claims 1-4, wherein tsaoko essential oil can be configured to be used for air as active ingredient
Pure and fresh solid, liquid, emulsion, spray and aerosol-type, wherein in spray propellant used be selected from dichlorodifluoromethan hydro carbons,
Hydrocarbon and compressed gas, and preferably, the form of spray is made in emulsion.
6. purposes of the tsaoko essential oil emulsion as air freshener and/or air bacteriostatic agent, the tsaoko essential oil emulsion is by weight
The composition of meter is as follows：
(1) 10 parts of the tsaoko essential oil in claim 1 to 5；
(2) solvent 20-35 parts；
(3) auxiliary agent 5-15 parts；With
(4) water 40-55 parts.
7. purposes according to claim 6, the solvent is selected from：Methanol, ethyl alcohol, n-butanol, normal propyl alcohol, isopropanol, isobutanol,
N-amyl alcohol, isoamyl alcohol, n-hexyl alcohol, acetone, dichloromethane, ethyl acetate, dimethyl sulfoxide, tetrahydrofuran, dimethylformamide, ring
Hexanone, pyrrolidones, the mixture of N-Methyl pyrrolidone or wherein two or more；Preferably, the solvent is selected from：
The mixture of ethyl alcohol, methanol, propyl alcohol, isopropanol, butanol, glycerine, acetone etc. or wherein two or more；Preferably, institute
State the mixture that solvent is methanol, ethyl alcohol or the two.
8. according to the purposes of claim 6 or 7, the auxiliary agent is surfactant, carrier and/or dispersant, and the surface is lived
Property agent be selected from Tweens, spans, lauryl sodium sulfate, alcohols, propylene glycol, polyethylene glycol and glycerine, dispersant choosing
From calgon, sodium pyrophosphate, polyacrylic acid sodium salt and polyvinyl alcohol, the carrier is selected from liposome, cyclodextrin and micro-capsule；
Preferably, the auxiliary agent is surfactant and/or carrier, and the surfactant is selected from Tweens, alkylphenol-polyethenoxy
Ethers, spans, lauryl sodium sulfate, the carrier are selected from liposome, cyclodextrin and micro-capsule.
9. purposes according to claim 6, the tsaoko essential oil emulsion is selected from：
By 1 part of tsaoko essential oil, 3.5 parts of ethyl alcohol, 1.5 parts of Tweens surfactant and 4 parts of emulsions for stirring evenly acquisition of water；
By 1 part of tsaoko essential oil, 2 parts of ethyl alcohol, 5.5 parts of 1.5 parts of alkylphenol polyoxyethylene ether class surfactant and water stir evenly
The emulsion of acquisition；
By 1 part of tsaoko essential oil, 3.5 parts of ethyl alcohol, 4 parts of 0.5 part of alkylphenol polyoxyethylene ether class surfactant and water stir evenly
The emulsion of acquisition；
By 1 part of tsaoko essential oil, 3.5 parts of ethyl alcohol, 4 parts of 1.5 parts of alkylphenol polyoxyethylene ether class surfactant and water stir evenly
The emulsion of acquisition；With
By 4 parts of 1 part of tsaoko essential oil, 2.5 parts of ethyl alcohol, 1 part of methanol, 1.5 parts of alkylphenol polyoxyethylene ether class surfactant and water
Stir evenly the emulsion of acquisition.
10. the use of tsaoko essential oil and tsaoko essential oil emulsion in following noxious air microorganism is inhibited in claim 1-9
On the way：Escherichia coli (Escherichia coli), staphylococcus aureus (Staphylococcus aureus), Candida albicans
Bacterium (Candida albicans), selected from Penicillium (Penicillium), aspergillus (Aspergillus) and Cladosporium
(Cladosporium) fungi.
11. purposes according to claim 10, the fungi of the aspergillus is aspergillus flavus (Aspergillus flavus), described
The fungi of Penicillium is Polish mould (Penicillium polonicum) and penicillium chrysogenum (Penicillium
Chrysogenum), the fungi of the Cladosporium is mould (Cladosporium cladosporioides) for dendritic branch spore.
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| CN113913245B (en) * | 2021-06-29 | 2023-10-10 | 中国热带农业科学院热带作物品种资源研究所 | Extraction method of fructus tsaoko essential oil for inhibiting candida tropicalis and fructus tsaoko essential oil |
| JP7447141B2 (en) | 2021-06-29 | 2024-03-11 | 中国▲熱▼▲帯▼▲農▼▲業▼科学院▲熱▼▲帯▼作物品▲種▼▲資▼源研究所 | Extraction method of Souka essential oil that inhibits Candida tropicalis and Souka essential oil |
| CN114532372A (en) * | 2022-02-18 | 2022-05-27 | 华南农业大学 | Application of amomum tsao-ko and waste thereof in preparation of plant source herbicide |
| CN114532372B (en) * | 2022-02-18 | 2022-08-12 | 华南农业大学 | Application of amomum tsao-ko and waste thereof in preparation of plant source herbicide |
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