CN108094716A - A kind of probiotic feed additive and preparation method thereof containing nanometer selenium, rare earth element - Google Patents
A kind of probiotic feed additive and preparation method thereof containing nanometer selenium, rare earth element Download PDFInfo
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- CN108094716A CN108094716A CN201711290246.7A CN201711290246A CN108094716A CN 108094716 A CN108094716 A CN 108094716A CN 201711290246 A CN201711290246 A CN 201711290246A CN 108094716 A CN108094716 A CN 108094716A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
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- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A23K40/00—Shaping or working-up of animal feeding-stuffs
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Abstract
The invention discloses a kind of probiotic feed additives and preparation method thereof containing nanometer selenium, rare earth element, and used raw materials by weight portion ratio is：58 parts of nanometer selenium raw material, 10 30 parts of rare earth element raw material, 30 60 parts of nano zine oxide raw material；Three of the above raw material mixed liquor is ground into 8000 mesh by electric mill, is ground again after filtering, fermentation, oxygen is passed through after filtering；It ferments again after sealing；It is again sealed off through water dilution, is mixed by 1: 50 weight ratio in feed, constantly stirs feed, be allowed to uniformly mixed；The feed per kg for adding the compound probiotic feed additive contains selenium element for 4,000 6000 μ g.Feed addictive of the present invention can effectively improve absorptivity and conversion ratio of the feeding animals to nutrient；Raw material is cheap and easy to get, process warm and easily realization；Materials Environmental Safety does not damage for animal body and human body；It is of great significance to aquaculture and food security.
Description
Technical field
It is especially a kind of to contain nanometer selenium, rare earth element the present invention relates to a kind of feed addictive and preparation method thereof
Probiotic feed additive and preparation method thereof.
Background technology
China is a selenium deficiency big country, and many place soil, drinking water selenium deficiency, there is Hubei Province in the high selenium area grasped at present
Bestow favour, there are abundant selenium resource, but the whole nation overwhelming majority in Lai Feng counties, Sichuan Guangyuan, the ground such as Yichun City lukewarm water town in Jiangxi Province
The serious selenium deficiency in area.Due to the natural quality of selenium, absorptivity is not high, along with China is in Feed Manufacturing, also still seriously deposits
In violated additives such as addition hormone, antibiotic, trichlorine cyanogen ammonia, the death rate of the poultry aquatic products in feeding process is higher, such as
For pig in feeding process, the death rate is usually 7% or so.
Scientific research proves, by the way that in the feed to poultry plus after selenium-supply and rare earth element, the white muscle disease of poultry obtains
Control, incidence are substantially reduced, and the speed of growth, the sorption enhanced rate of feed are largely increased, and have saved grain.Generally it is in
Fowl (is less than 0.016mg) in the case of lacking selenium element per kg body weight selenium element content, by adding every kilogram of selenium-supply element
Weight selenium element content reaches 0.1mg, rare earth element (cerium, lanthanum, praseodymium) content reaches 20mg, can make the life of laying rate, chick
Power all increases, and more prominent is that the quality of egg and the protein density freshness of muscle all have clear improvement, and makes poultry
Poultry raising has gone up the New step of a green safe high quality again.
Selenium element and rare earth element are added in feed, and the effect in aquaculture is very significant.Such as in cattle-raising
In, the output of milk and daily gain can be improved by feeding ox using selenium element and rare earth element composite additive, and be saved food, and reduced and raised
Form this.It is confirmed in practice, adds the rare earth element of 100mg and 800mg, two groups of milk cow difference in every kilogram of dairy cow diet respectively
The daily ration of intake addition 100mg and 800mg rare earth elements, the results show that two groups of milk production of cow comparisons are respectively increased 9.27%
With 21.476%, 0.36% and 0.45% is respectively increased in butterfat percnetage.Rare earth element and selenium are added in poultry, fowl, aquaculture feeds
Element, it is also of great advantage to the cultivation of poultry, fowl, aquatic products etc..
After domestic animals and fowls are taken in, absorptivity is still undesirable, how to carry for addition selenium element, the feed of rare earth element at present
This technical problem of the absorptivity of the nutriments such as selenium element, rare earth element is urgently to be resolved hurrily in high feed.
The content of the invention
To solve above existing problem, the invention discloses a kind of probiotic feeds containing nanometer selenium, rare earth element to add
Add agent and preparation method thereof, what concrete scheme was realized in：
A kind of probiotic feed additive and preparation method thereof containing nanometer selenium, rare earth element, used raw material is pressed
Ratio of weight and number is：5-8 parts of nanometer selenium raw material, 10-30 parts of rare earth element raw material, 30-60 parts of nano zine oxide raw material；
A kind of probiotic feed additive and preparation method thereof containing nanometer selenium, rare earth element, step is as follows：
(1) three of the above raw material mixed liquor is ground into 8000 mesh by electric mill, is realized using nanofiltration device
The nanosizing of feed particles；Raw material mixed liquor after filtering ferment in the sun 10-14 days to pH be 3.6-4.0；
(2) the raw material mixed liquor through everfermentation for obtaining step (1) is ground again by electric mill, the original obtained after grinding
Material mixed liquor is passed through oxygen after being filtered with nanofiltration device, when the time is 10 small；The raw material mixed liquor obtained after logical oxygen is put
Enter in sterile chamber and be sealed, under illumination condition, be placed in 31 DEG C of environment and ferment 5 days；Then mixed toward equipped with raw material
The water for the 10 times of volumes of raw material mixed liquor fermented is added in the container of liquid, is again sealed off, is deflated once within every 4 days；So obtain
Compound probiotic feed additive；
(3) the compound probiotic feed additive for obtaining step (2) is mixed by 1: 50 weight ratio in feed；
The compound probiotic feed additive that step (2) is obtained is sprayed at the feed table to be added after being diluted with pure water
Then feed is constantly stirred in face, be uniformly mixed the compound probiotic feed additive and feed, generally with " hand one is pinched
Agglomerating, hand is scattered once letting go " it is advisable；The compound probiotic feed additive and quality of the fodder ratio are 1: (2000-
2500) feed per kg for, adding the compound probiotic feed additive contains selenium element for 4000-6000 μ g.
Further, the nanometer selenium raw material is obtained by：To be selenium dioxide 0.005- by ratio of weight and the number of copies
0.008 part, 10 parts of rare earth element, 0.2-40 parts of chitosan, 20 parts of L-lysine, 18 parts of cysteine, 100 parts of Nutrient medium is red
100 parts of sugar, glucose and honey mixt, 0.001 part of vitamin C, 0.001 part of sodium bicarbonate, 0.0001 part of potassium dihydrogen phosphate
All raw materials are uniformly mixed, and add in the water of 25-30 times of raw material gross mass, and are stirred evenly, and obtain the suspension of raw material；It adds
10% effective microorganisms(EM) of the suspension quality of raw material simultaneously stirs evenly, and obtains nanometer selenium feed stock solutions；By the nanometer selenium raw material
Stoste is poured into electronic levigator and is ground, and grinds to 5000 mesh；The nanometer selenium feed stock solutions after grinding pass through nanometer
After grade filter device filtering, be passed through oxygen 10 it is small when, then be sealed fermentation, temperature is controlled between 28-35 DEG C, is fermented
It is 3.6 ± 0.2 to pH value；Wherein, the selenium dioxide is SILVER REAGENT；The selenium dioxide can also use selenium dioxide and Asia
Sodium selenate mixture replaces, and the mass ratio of selenium dioxide and sodium selenite is 1: 1.5 in selenium dioxide and sodium selenite mixture；
The Nutrient medium is the Nutrient medium containing Golden flower ferment；The effective microorganisms(EM) is to contain lactobacillus, Xinghe flora, yeast flora
Microorganism formulation.
Further, the rare earth element raw material is obtained by：By the fluorine carbon containing rare earth elements such as lanthanum, cerium, praseodymiums
After cerium miberal powder end carries out primary attrition process, put it into electronic levigator and ground；It is carried out again through nanofiltration device
Repeated filtration, reaches 5000 mesh fine degrees, obtains the rare earth element feed stock solutions；Then the rare earth element raw material is added in
The Nutrient medium of stock solution quality 30% adds in the effective microorganisms(EM) of the rare earth element feed stock solutions quality 10%, adds in the rare earth member
Brown sugar, honey and the glucose mixture of plain feed stock solutions quality 30%, object mixed above is stirred evenly, in 20-26 DEG C of item
It ferments under part, is considered as when Surface Creation white hair bacterium and ferments successfully；Wherein, the bastnaesite powder also can be by fluorine carbon calcium
The mixture of one or more of cerium ore deposit, fluorine carbon cerium calcium ore deposit, cordylite and monazite replaces.
Further, the nano zine oxide raw material is obtained by：To be 2 parts of zinc oxide by ratio of weight and the number of copies, Portugal
Grape sugar, 4 parts of brown sugar and honey mixt, 6 parts of Nutrient medium, 1 part of effective microorganisms(EM), all materials that 26 parts of water stir into close after suspension
Envelope, in 28-35 DEG C of conditioned response 10 days or more；The suspension after fermentation is ground into 5000 mesh with electric mill repeatedly again；Finally adopt
It is filtered with nanofiltration device, obtains the nano zine oxide raw material of nanosizing；Wherein, zinc oxide is nanoscale,
Purity is 99.99%；The material composition of Nutrient medium includes by ratio of weight and the number of copies：20 parts of L-lysine, 2 parts of methionine, chitosan
0.2 part, 2 parts of concentration powder enzyme, 1 part of vitamin C, 3 parts of potassium dihydrogen phosphate additive；Water is alkalescent water.
Advantageous effect：Feed addictive of the present invention can effectively improve absorptivity of the feeding animals to nutrient
And conversion ratio；Raw material is cheap and easy to get, process warm and easily realization；Materials Environmental Safety does not damage for animal body and human body；
It is of great significance to aquaculture and food security.
Specific embodiment
In conjunction with the embodiments, the present invention is described in further details.
Embodiment 1
A kind of probiotic feed additive and preparation method thereof containing nanometer selenium, rare earth element, using following steps：
(1) preparation of nanometer selenium raw material
To be 0.005 part of selenium dioxide by ratio of weight and the number of copies, 10 parts of rare earth element, 0.2 part of chitosan, L-lysine 20
Part, 18 parts of cysteine, 100 parts of Nutrient medium, 100 parts of brown sugar, glucose and honey mixt, 0.001 part of vitamin C, little Su
0.001 part is made a call to, all raw materials of 0.0001 part of potassium dihydrogen phosphate are uniformly mixed, and add in the water of 30 times of raw material gross mass, and are stirred
Uniformly, the suspension of raw material is obtained；It adds 10% effective microorganisms(EM) of the suspension quality of raw material and stirs evenly, obtain nanometer
Selenium feed stock solutions；The nanometer selenium feed stock solutions are poured into electronic levigator to grind, are ground to 5000 mesh；After grinding
After the nanometer selenium feed stock solutions are filtered by nanofiltration device, be passed through oxygen 10 it is small when, then be sealed fermentation,
Temperature is controlled between 32 DEG C, and it is 3.6 to ferment to pH value；Wherein, the selenium dioxide is SILVER REAGENT；The selenium dioxide also may be used
It is replaced with selenium dioxide and sodium selenite mixture, selenium dioxide and sodium selenite in selenium dioxide and sodium selenite mixture
Mass ratio is 1: 1.5；The Nutrient medium is the Nutrient medium containing Golden flower ferment；The effective microorganisms(EM) is to contain lactobacillus, emerging
With flora, the microorganism formulation of yeast flora.
(2) preparation of rare earth element raw material
After bastnaesite powder containing rare earth elements such as lanthanum, cerium, praseodymiums is carried out primary attrition process, put it into electronic
Levigator is ground；It is repeatedly filtered again through nanofiltration device, reaches 5000 mesh fine degrees, obtain the rare earth
Element feed stock solutions；Then the Nutrient medium of the rare earth element feed stock solutions quality 30% is added in, it is former to add in the rare earth element
Expect the effective microorganisms(EM) of stock solution quality 10%, brown sugar, honey and the glucose for adding in the rare earth element feed stock solutions quality 30% mix
Object is closed, object mixed above is stirred evenly, is fermented under the conditions of 26 DEG C, is considered as when Surface Creation white hair bacterium and is fermented into
Work(；Wherein, the bastnaesite powder also can be by one in parisite, fluorine carbon cerium calcium ore deposit, cordylite and monazite
Kind or several mixtures replace.
(3) preparation of nano zine oxide raw material
To be 2 parts of zinc oxide by ratio of weight and the number of copies, 4 parts of glucose, brown sugar and honey mixt, 6 parts of Nutrient medium, effective microorganisms(EM)
1 part, all materials that 26 parts of water seal after stirring into suspension, in 32 DEG C of conditioned responses 10 days or more；It again will be mixed after fermentation
Suspension is ground into 5000 mesh with electric mill repeatedly；It is finally filtered using nanofiltration device, obtains the described of nanosizing and receive
Rice crude zinc oxide materials；Wherein, zinc oxide is nanoscale, purity 99.99%；The material composition of Nutrient medium wraps by ratio of weight and the number of copies
It includes：20 parts of L-lysine, 2 parts of methionine, 0.2 part of chitosan, 2 parts of concentration powder enzyme, 1 part of vitamin C, potassium dihydrogen phosphate addition
3 parts of agent；Water is alkalescent water.
(4) the nanometer selenium raw material, the rare earth element raw material, the nano zine oxide obtained three above step
Raw materials by weight portion ratio is：5-8 parts of nanometer selenium raw material, 10-30 parts of rare earth element raw material, 30-60 parts of nano zine oxide raw material
Proportioning mixing, obtains mixed liquor；Mixed liquor is ground into 8000 mesh by electric mill, raw material is realized using nanofiltration device
The nanosizing of particle；Raw material mixed liquor after filtering ferment in the sun 12 days to pH be 3.8.
(5) the raw material mixed liquor through everfermentation for obtaining previous step is ground again by electric mill, is obtained after grinding
Raw material mixed liquor is passed through oxygen after being filtered with nanofiltration device, when the time is 10 small；The raw material mixed liquor obtained after logical oxygen
It is put into sterile chamber and is sealed, under illumination condition, be placed in 31 DEG C of environment and ferment 5 days；Then it is past to be mixed equipped with raw material
The water that the 10 times of volumes of raw material mixed liquor fermented are added in the container of liquid is closed, is again sealed off, is deflated once within every 4 days；Such
To compound probiotic feed additive；
(6) the compound probiotic feed additive for obtaining previous step is mixed into feed by 1: 50 weight ratio
In；The compound probiotic feed additive that step (2) is obtained is sprayed at the feed to be added after being diluted with pure water
Then feed is constantly stirred on surface, be uniformly mixed the compound probiotic feed additive and feed, generally with " hand one
Pinch agglomerating, hand and be scattered once letting go " it is advisable；The compound probiotic feed additive and quality of the fodder ratio are 1: 2000,
The feed per kg for adding the compound probiotic feed additive contains selenium element for 4000 μ g.
The compound probiotic feed additive made above is suitble to the cultivation of poultry, egg production after feeding one month
It is improved with micronutrient levels in meat.
Embodiment 2
A kind of probiotic feed additive and preparation method thereof containing nanometer selenium, rare earth element, using following steps：
(1) preparation of nanometer selenium raw material
To be 0.008 part of selenium dioxide by ratio of weight and the number of copies, 10 parts of rare earth element, 2 parts of chitosan, 20 parts of L-lysine,
18 parts of cysteine, 100 parts of Nutrient medium, 100 parts of brown sugar, glucose and honey mixt, 0.001 part of vitamin C, sodium bicarbonate
0.001 part, all raw materials of 0.0001 part of potassium dihydrogen phosphate are uniformly mixed, and add in the water of 25 times of raw material gross mass, and are stirred equal
It is even, obtain the suspension of raw material；It adds 10% effective microorganisms(EM) of the suspension quality of raw material and stirs evenly, obtain nanometer selenium
Feed stock solutions；The nanometer selenium feed stock solutions are poured into electronic levigator to grind, are ground to 5000 mesh；Institute after grinding
State after nanometer selenium feed stock solutions are filtered by nanofiltration device, be passed through oxygen 10 it is small when, then be sealed fermentation, it is warm
Between 30 DEG C, it is 3.6 to ferment to pH value for degree control；Wherein, the selenium dioxide is SILVER REAGENT；The selenium dioxide also can use
Selenium dioxide and sodium selenite mixture replace, the matter of selenium dioxide and sodium selenite in selenium dioxide and sodium selenite mixture
Amount is than being 1: 1.5；The Nutrient medium is the Nutrient medium containing Golden flower ferment；The effective microorganisms(EM) is to contain lactobacillus, Xinghe
The microorganism formulation of flora, yeast flora.
(2) preparation of rare earth element raw material
After bastnaesite powder containing rare earth elements such as lanthanum, cerium, praseodymiums is carried out primary attrition process, put it into electronic
Levigator is ground；It is repeatedly filtered again through nanofiltration device, reaches 5000 mesh fine degrees, obtain the rare earth
Element feed stock solutions；Then the Nutrient medium of the rare earth element feed stock solutions quality 30% is added in, it is former to add in the rare earth element
Expect the effective microorganisms(EM) of stock solution quality 10%, brown sugar, honey and the glucose for adding in the rare earth element feed stock solutions quality 30% mix
Object is closed, object mixed above is stirred evenly, is fermented under the conditions of 25 DEG C, is considered as when Surface Creation white hair bacterium and is fermented into
Work(；Wherein, the bastnaesite powder also can be by one in parisite, fluorine carbon cerium calcium ore deposit, cordylite and monazite
Kind or several mixtures replace.
(3) preparation of nano zine oxide raw material
To be 2 parts of zinc oxide by ratio of weight and the number of copies, 4 parts of glucose, brown sugar and honey mixt, 6 parts of Nutrient medium, effective microorganisms(EM)
1 part, all materials that 26 parts of water seal after stirring into suspension, in 32 DEG C of conditioned responses 10 days or more；It again will be mixed after fermentation
Suspension is ground into 5000 mesh with electric mill repeatedly；It is finally filtered using nanofiltration device, obtains the described of nanosizing and receive
Rice crude zinc oxide materials；Wherein, zinc oxide is nanoscale, purity 99.99%；The material composition of Nutrient medium wraps by ratio of weight and the number of copies
It includes：20 parts of L-lysine, 2 parts of methionine, 0.2 part of chitosan, 2 parts of concentration powder enzyme, 1 part of vitamin C, potassium dihydrogen phosphate addition
3 parts of agent；Water is alkalescent water.
(4) the nanometer selenium raw material, the rare earth element raw material, the nano zine oxide obtained three above step
Raw materials by weight portion ratio is：8 parts of nanometer selenium raw material, 30 parts of rare earth element raw material, the 60 parts of proportioning mixing of nano zine oxide raw material,
Obtain mixed liquor；Mixed liquor is ground into 8000 mesh by electric mill, receiving for feed particles is realized using nanofiltration device
Meter Hua；Raw material mixed liquor after filtering ferment in the sun 14 days to pH be 3.8.
(5) the raw material mixed liquor through everfermentation for obtaining previous step is ground again by electric mill, is obtained after grinding
Raw material mixed liquor is passed through oxygen after being filtered with nanofiltration device, when the time is 10 small；The raw material mixed liquor obtained after logical oxygen
It is put into sterile chamber and is sealed, under illumination condition, be placed in 31 DEG C of environment and ferment 5 days；Then it is past to be mixed equipped with raw material
The water that the 10 times of volumes of raw material mixed liquor fermented are added in the container of liquid is closed, is again sealed off, is deflated once within every 4 days；Such
To compound probiotic feed additive；
(6) the compound probiotic feed additive for obtaining previous step is mixed into feed by 1: 50 weight ratio
In；The compound probiotic feed additive that step (2) is obtained is sprayed at the feed to be added after being diluted with pure water
Then feed is constantly stirred on surface, be uniformly mixed the compound probiotic feed additive and feed, generally with " hand one
Pinch agglomerating, hand and be scattered once letting go " it is advisable；The compound probiotic feed additive and quality of the fodder ratio are 1: 2200,
The feed per kg for adding the compound probiotic feed additive contains selenium element for 6000 μ g.
The compound probiotic feed additive made above is suitble to the cultivation of domestic animal especially milk cow, feeds one month
Micronutrient levels greatly improves in the output of milk and meat afterwards.
Embodiment 3
A kind of probiotic feed additive and preparation method thereof containing nanometer selenium, rare earth element, using following steps：
(1) preparation of nanometer selenium raw material
To be 0.006 part of selenium dioxide by ratio of weight and the number of copies, 10 parts of rare earth element, 0.8 part of chitosan, L-lysine 20
Part, 18 parts of cysteine, 100 parts of Nutrient medium, 100 parts of brown sugar, glucose and honey mixt, 0.001 part of vitamin C, little Su
0.001 part is made a call to, all raw materials of 0.0001 part of potassium dihydrogen phosphate are uniformly mixed, and add in the water of 28 times of raw material gross mass, and are stirred
Uniformly, the suspension of raw material is obtained；It adds 10% effective microorganisms(EM) of the suspension quality of raw material and stirs evenly, obtain nanometer
Selenium feed stock solutions；The nanometer selenium feed stock solutions are poured into electronic levigator to grind, are ground to 5000 mesh；After grinding
After the nanometer selenium feed stock solutions are filtered by nanofiltration device, be passed through oxygen 10 it is small when, then be sealed fermentation,
Temperature is controlled between 34 DEG C, and it is 3.6 to ferment to pH value；Wherein, the selenium dioxide is SILVER REAGENT；The selenium dioxide also may be used
It is replaced with selenium dioxide and sodium selenite mixture, selenium dioxide and sodium selenite in selenium dioxide and sodium selenite mixture
Mass ratio is 1: 1.5；The Nutrient medium is the Nutrient medium containing Golden flower ferment；The effective microorganisms(EM) is to contain lactobacillus, emerging
With flora, the microorganism formulation of yeast flora.
(2) preparation of rare earth element raw material
After bastnaesite powder containing rare earth elements such as lanthanum, cerium, praseodymiums is carried out primary attrition process, put it into electronic
Levigator is ground；It is repeatedly filtered again through nanofiltration device, reaches 5000 mesh fine degrees, obtain the rare earth
Element feed stock solutions；Then the Nutrient medium of the rare earth element feed stock solutions quality 30% is added in, it is former to add in the rare earth element
Expect the effective microorganisms(EM) of stock solution quality 10%, brown sugar, honey and the glucose for adding in the rare earth element feed stock solutions quality 30% mix
Object is closed, object mixed above is stirred evenly, is fermented under the conditions of 26 DEG C, is considered as when Surface Creation white hair bacterium and is fermented into
Work(；Wherein, the bastnaesite powder also can be by one in parisite, fluorine carbon cerium calcium ore deposit, cordylite and monazite
Kind or several mixtures replace.
(3) preparation of nano zine oxide raw material
To be 2 parts of zinc oxide by ratio of weight and the number of copies, 4 parts of glucose, brown sugar and honey mixt, 6 parts of Nutrient medium, effective microorganisms(EM)
1 part, all materials that 26 parts of water seal after stirring into suspension, in 33 DEG C of conditioned responses 10 days or more；It again will be mixed after fermentation
Suspension is ground into 5000 mesh with electric mill repeatedly；It is finally filtered using nanofiltration device, obtains the described of nanosizing and receive
Rice crude zinc oxide materials；Wherein, zinc oxide is nanoscale, purity 99.99%；The material composition of Nutrient medium wraps by ratio of weight and the number of copies
It includes：20 parts of L-lysine, 2 parts of methionine, 0.2 part of chitosan, 2 parts of concentration powder enzyme, 1 part of vitamin C, potassium dihydrogen phosphate addition
3 parts of agent；Water is alkalescent water.
(4) the nanometer selenium raw material, the rare earth element raw material, the nano zine oxide obtained three above step
Raw materials by weight portion ratio is：7 parts of nanometer selenium raw material, 25 parts of rare earth element raw material, the 50 parts of proportioning mixing of nano zine oxide raw material,
Obtain mixed liquor；Mixed liquor is ground into 8000 mesh by electric mill, receiving for feed particles is realized using nanofiltration device
Meter Hua；Raw material mixed liquor after filtering ferment in the sun 13 days to pH be 3.8.
(5) the raw material mixed liquor through everfermentation for obtaining previous step is ground again by electric mill, is obtained after grinding
Raw material mixed liquor is passed through oxygen after being filtered with nanofiltration device, when the time is 10 small；The raw material mixed liquor obtained after logical oxygen
It is put into sterile chamber and is sealed, under illumination condition, be placed in 31 DEG C of environment and ferment 5 days；Then it is past to be mixed equipped with raw material
The water that the 10 times of volumes of raw material mixed liquor fermented are added in the container of liquid is closed, is again sealed off, is deflated once within every 4 days；Such
To compound probiotic feed additive；
(6) the compound probiotic feed additive for obtaining previous step is mixed into feed by 1: 50 weight ratio
In；The compound probiotic feed additive that step (2) is obtained is sprayed at the feed to be added after being diluted with pure water
Then feed is constantly stirred on surface, be uniformly mixed the compound probiotic feed additive and feed, generally with " hand one
Pinch agglomerating, hand and be scattered once letting go " it is advisable；The compound probiotic feed additive and quality of the fodder ratio are 1: 2300,
The feed per kg for adding the compound probiotic feed additive contains selenium element for 5500 μ g.
The compound probiotic feed additive made above is suitble to the cultivation of aquatic products especially fish, feeds one month
Micronutrient levels is improved in the speed of growth and meat afterwards.
Above-mentioned specific embodiment is only intended to clearly illustrate example of the present invention, and not to embodiment
It limits.For those of ordinary skill in the art, other various forms can also be made on the basis of the above description
Variation or variation, there is no necessity and possibility to exhaust all the enbodiments, and thus amplifies out apparent
Variation or change still in protection scope of the present invention.
Claims (10)
1. a kind of probiotic feed additive and preparation method thereof containing nanometer selenium, rare earth element, which is characterized in that used
Raw materials by weight portion ratio be：5-8 parts of nanometer selenium raw material, 10-30 parts of rare earth element raw material, nano zine oxide raw material 30-60
Part；
A kind of probiotic feed additive and preparation method thereof containing nanometer selenium, rare earth element, step is as follows：
(1) three of the above raw material mixed liquor is ground into 8000 mesh by electric mill, raw material is realized using nanofiltration device
The nanosizing of particle；Raw material mixed liquor after filtering ferment in the sun 10-14 days to pH be 3.6-4.0；
(2) the raw material mixed liquor through everfermentation for obtaining step (1) is ground again by electric mill, and the raw material obtained after grinding mixes
It closes and oxygen is passed through after liquid is filtered with nanofiltration device, when the time is 10 small；The raw material mixed liquor obtained after logical oxygen is put into nothing
It is sealed in bacterium container, under illumination condition, is placed in 31 DEG C of environment and ferments 5 days；Then toward equipped with raw material mixed liquor
The water for the 10 times of volumes of raw material mixed liquor fermented is added in container, is again sealed off, is deflated once within every 4 days；It so obtains compound
Type probiotic feed additive；
(3) the compound probiotic feed additive for obtaining step (2) is mixed by 1: 50 weight ratio in feed；It will step
Suddenly the compound probiotic feed additive that (2) obtain is sprayed at the feed surface to be added after being diluted with pure water, so
Feed is constantly stirred afterwards, is uniformly mixed the compound probiotic feed additive and feed；The compound probiotics is raised
Feed additives and quality of the fodder ratio are 1: (2000-2500), the feed for adding the compound probiotic feed additive are every
Kilogram containing selenium element be 4000-6000 μ g.
A kind of contain nanometer selenium, the probiotic feed additive of rare earth element and its preparation side 2. according to claim 1
Method, which is characterized in that the nanometer selenium raw material was obtained by：To be selenium dioxide 0.005-0.008 by ratio of weight and the number of copies
Part, 10 parts of rare earth element, 0.2-40 parts of chitosan, 20 parts of L-lysine, 18 parts of cysteine, 100 parts of Nutrient medium, brown sugar, Portugal
100 parts of grape sugar and honey mixt, 0.001 part of vitamin C, 0.001 part of sodium bicarbonate, 0.0001 part of potassium dihydrogen phosphate own
Raw material is uniformly mixed, and adds in the water of 25-30 times of raw material gross mass, and is stirred evenly, and obtains the suspension of raw material；Add raw material
Suspension quality 10% effective microorganisms(EM) and stir evenly, obtain nanometer selenium feed stock solutions；By the nanometer selenium feed stock solutions
It pours into electronic levigator to be ground, grind to 5000 mesh；The nanometer selenium feed stock solutions after grinding pass through nanoscale mistake
After filtering device filtering, be passed through oxygen 10 it is small when, then be sealed fermentation, temperature control is fermented between 28-35 DEG C to pH
It is worth for 3.6 ± 0.2.
A kind of contain nanometer selenium, the probiotic feed additive of rare earth element and its preparation side 3. according to claim 2
Method, which is characterized in that the selenium dioxide can also be replaced with selenium dioxide and sodium selenite mixture, selenium dioxide and selenous acid
The mass ratio of selenium dioxide and sodium selenite is 1: 1.5 in sodium mixture.
A kind of contain nanometer selenium, the probiotic feed additive of rare earth element and its preparation side 4. according to claim 2
Method, which is characterized in that the Nutrient medium is the Nutrient medium containing Golden flower ferment.
A kind of contain nanometer selenium, the probiotic feed additive of rare earth element and its preparation side 5. according to claim 1
Method, which is characterized in that the rare earth element raw material was obtained by：By the bastnaesite containing rare earth elements such as lanthanum, cerium, praseodymiums
After powder carries out primary attrition process, put it into electronic levigator and ground；It is carried out repeatedly through nanofiltration device again
Filtering, reaches 5000 mesh fine degrees, obtains the rare earth element feed stock solutions；Then the rare earth element feed stock solutions are added in
The Nutrient medium of quality 30% adds in the effective microorganisms(EM) of the rare earth element feed stock solutions quality 10%, and it is former to add in the rare earth element
Expect brown sugar, honey and the glucose mixture of stock solution quality 30%, object mixed above is stirred evenly, under the conditions of 20-26 DEG C
It ferments, is considered as when Surface Creation white hair bacterium and ferments successfully.
A kind of contain nanometer selenium, the probiotic feed additive of rare earth element and its preparation side 6. according to claim 5
Method, which is characterized in that the bastnaesite powder also can be by parisite, fluorine carbon cerium calcium ore deposit, cordylite and monazite
One or more of mixture replace.
A kind of contain nanometer selenium, the probiotic feed additive of rare earth element and its preparation side 7. according to claim 1
Method, which is characterized in that the nano zine oxide raw material was obtained by：To be 2 parts of zinc oxide by ratio of weight and the number of copies, grape
Sugar, 4 parts of brown sugar and honey mixt, 6 parts of Nutrient medium, 1 part of effective microorganisms(EM), all materials that 26 parts of water stir into close after suspension
Envelope, in 28-35 DEG C of conditioned response 10 days or more；The suspension after fermentation is ground into 5000 mesh with electric mill repeatedly again；Finally adopt
It is filtered with nanofiltration device, obtains the nano zine oxide raw material of nanosizing.
A kind of contain nanometer selenium, the probiotic feed additive of rare earth element and its preparation side 8. according to claim 7
Method, which is characterized in that the zinc oxide be nanoscale, purity 99.99%.
A kind of contain nanometer selenium, the probiotic feed additive of rare earth element and its preparation side 9. according to claim 7
Method, which is characterized in that the material composition of the Nutrient medium includes by ratio of weight and the number of copies：20 parts of L-lysine, 2 parts of methionine, shell
0.2 part of glycan, 2 parts of concentration powder enzyme, 1 part of vitamin C, 3 parts of potassium dihydrogen phosphate additive.
A kind of contain nanometer selenium, the probiotic feed additive of rare earth element and its preparation side 10. according to claim 7
Method, which is characterized in that the water is alkalescent water.
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| CN111202185A (en) * | 2020-01-07 | 2020-05-29 | 香港科技大学深圳研究院 | Additive-containing feed for siganus and nutrition detection method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101313729A (en) * | 2008-06-20 | 2008-12-03 | 田福平 | Organic biological feedstuff additive containing nano-selenium, zinc oxide, preparation and use method thereof |
| CN103416597A (en) * | 2012-05-25 | 2013-12-04 | 鹤山市雷迅新农机械科技有限公司 | Preparation method of animal forage microbe organic rare earth |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101313729A (en) * | 2008-06-20 | 2008-12-03 | 田福平 | Organic biological feedstuff additive containing nano-selenium, zinc oxide, preparation and use method thereof |
| CN103416597A (en) * | 2012-05-25 | 2013-12-04 | 鹤山市雷迅新农机械科技有限公司 | Preparation method of animal forage microbe organic rare earth |
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| CN111202185A (en) * | 2020-01-07 | 2020-05-29 | 香港科技大学深圳研究院 | Additive-containing feed for siganus and nutrition detection method thereof |
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