CN108077253A - Application of the C6 volatile Aldehyde components in strawberry spherical cavity bacterium is inhibited - Google Patents
Application of the C6 volatile Aldehyde components in strawberry spherical cavity bacterium is inhibited Download PDFInfo
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- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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Abstract
The invention discloses application of the C6 volatile Aldehyde components in strawberry spherical cavity bacterium is inhibited, which is hexanal, trans 2 hexenoic aldehyde or cis 3 hexenoic aldehyde.Present invention firstly discloses hexanal, trans 2 hexenoic aldehyde and cis 3 hexenoic aldehyde these three C6 volatile Aldehyde components in Strawberry Leaves can inhibit strawberry spherical cavity bacterium, the especially spore germination of strawberry spherical cavity bacterium and mycelial growth, and the experiment of strawberry spherical cavity bacterium or Strawberry Leaves is fumigated by using the C6 volatile Aldehyde components, hexanal, trans 2 hexenoic aldehyde and cis 3 hexenoic aldehyde are demonstrated in the effect in effectively inhibiting the spore germination and mycelial growth of strawberry spherical cavity bacterium and the function in prevention white leaf spot of strawberry.The present invention prepares the preparation of the prevention microbial white leaf spot of strawberry of strawberry spherical cavity using hexanal, trans 2 hexenoic aldehyde and cis 3 hexenoic aldehyde, and carry out white leaf spot of strawberry disease control, control effect is apparent, the usage amount of pesticide can be effectively reduced, the biological control method to develop new, efficient, green safe prevention white leaf spot of strawberry provides foundation.
Description
Technical field
The invention belongs to technical field of agriculture science more particularly to C6 volatile Aldehyde components in strawberry spherical cavity bacterium is inhibited
Application.
Background technology
Strawberry spherical cavity bacterium (M.fragariae) is the pathogen that white leaf spot of strawberry is caused to do harm to, also known as the small spherical cavity of strawberry snake eye
Bacterium, belong to Ascomycotina chamber Pseudomonas fungi, Invisible element conidiophore grows thickly, shorter, filbert or colourless, branch or regardless of
Branch, base portion stroma are undeveloped.The oval tubular of conidium generally has 1~2 membrane, cell inclusion granule to spindle-like.
White leaf spot of strawberry is also known as strawberry Leucoplakia, leaf spot, and main harm blade falls ill mostly in old leaf and causes scab,
Also petiole, carpopodium, calyx, stolon and carpopodium are infected.There are many subcircular, purples to differ in size on blade in early stage
Red stigma, like snake eye shape.Afterwards, central spot be transformed into it is greyish white to taupe and form blotch, also aubergine sometimes
Take turns line.When humidity is high, scab surface generates the mould layer of white powder, i.e. germ conidiophore and conidium.Later stage is in scab
On can produce many pores, i.e. germ ascostome.During serious generation, scab gathers on blade, and morbidity leaf is in the short time
Necrosis is dried-up.White leaf spot of strawberry is very big to the harm of strawberry fruit, can seriously cause to drop in production over a large area.
At present, the controlling mode of white leaf spot of strawberry except wheel for ploughing does, lowering of watertable, ventilated, cleaning sick seedling,
It extracts outside the traditional field cultivation means of sick leaf and fertilizition control etc., mainly or based on chemical control, such as Bordeaux mixture, generation
Gloomy MnZn green must protect suspending agent, network ammonia copper water, can kill, the agrochemicals such as Bravo, thiophanate methyl and carbendazim.But by
Caused by long-time service in chemical bactericide the problems such as pesticide residue, environmental pollution, food security, the pathogen resistance to the action of a drug increasingly
Seriously, so, research and develop safe and efficient biological bactericide and be of great significance.
Plant itself can generate a variety of volatile materials, these volatile materials as airborne signal chemical, plant with
Very important effect is played in interaction between environment, plant and disease and plant and plant etc..Recently, research both at home and abroad
Show that plant can rise by internal volatile matter content after pathogen infection or generation mechanical damage, C6 aldehydes volatility objects
Matter is one type key components, confirms that these substances play an important role in disease resistance of plant on evidence
(Dudareva,Klempienet al.2013)。
He Peiqing etc. (2005) reviews the plant of volatile materials or component with antibacterial activity and corresponding cause of disease
Bacterium, but there is inhibitory activity for the active volatile ingredient in Strawberry Leaves and to strawberry spherical cavity bacterium (M.fragariae)
Volatile materials is not reported for work.Publication No. is that the application for a patent for invention document of CN104920359A discloses a kind of efficient prevention and control water
The plants essential oil of rice rice blast, principle active component are citral and citronellal.Publication No. is the invention of CN105123911A
Patent application discloses a kind of method for adopting rear gray mold using linalool prevention blueberry.
The content of the invention
It is an object of the invention to provide application of the C6 volatile Aldehyde components in strawberry spherical cavity bacterium is inhibited, the C6 aldehydes
Volatile materials can effectively inhibit spore germination and the mycelial growth of strawberry spherical cavity bacterium, and then it is microbial to prevent strawberry spherical cavity
White leaf spot of strawberry prepares pesticide control with existing C6 volatile Aldehyde components in such Strawberry Leaves, can have efficient, peace
The features such as complete and environmental-friendly, overcome deficiency of the prior art.
Particular technique content is as follows：
The present invention provides application of the C6 volatile Aldehyde components in strawberry spherical cavity bacterium is inhibited, the C6 aldehydes volatility
Substance is hexanal, trans -2- hexenoic aldehydes or cis -3- hexenoic aldehydes.
Further, C6 volatile Aldehyde components can inhibit spore germination and the mycelial growth of strawberry spherical cavity bacterium.
The present invention is using main C6 volatile Aldehyde components hexanal, trans -2- hexenoic aldehydes and cis -3- in Strawberry Leaves
Hexenoic aldehyde is tested, since hexanal, trans -2- hexenoic aldehydes and cis -3- hexenoic aldehydes are volatile materials, so in antibacterial reality
Test in disease control using carry out under closed environment it is stifling by the way of handled, found through experiment, certain
Under concentration range hexanal, trans -2- hexenoic aldehydes and cis -3- hexenoic aldehydes can effectively inhibit strawberry spherical cavity bacterium spore germination and
Mycelial growth.
Wherein, in the spore germination for inhibiting strawberry spherical cavity bacterium, with the body of the periphery closed environment where strawberry spherical cavity bacterium
Product meter, the μ L/L of the concentration of the hexanal >=0.5, the concentration of the trans -2- hexenoic aldehydes >=0.05 μ L/L, the cis -3- oneself
The μ L/L of the concentration of olefine aldehydr >=0.5.
In the mycelial growth for inhibiting strawberry spherical cavity bacterium, with the stereometer of the periphery closed environment where strawberry spherical cavity bacterium,
The μ L/L of the concentration of the hexanal >=0.05, the μ L/L of the concentration of the trans -2- hexenoic aldehydes >=0.05, the cis -3- hexenoic aldehydes
The μ L/L of concentration >=0.05.
When the concentration of hexanal and cis -3- hexenoic aldehydes reaches 5 μ L/L, when trans -2- hexenoic aldehydes reach 0.5 μ L/L, can have
Effect inhibits spore germination, and spore germination rate declines 80-99%；There is C6 volatile Aldehyde components higher than above-mentioned concentration sterilization to make
With, i.e., volatile materials is fumigated remove volatile materials after 16h after, spore, which continues to cultivate 72h, to be still failed to sprout.Above-mentioned C6 aldehyde
The low dosage of class volatile materials fumigate can also significantly inhibit sprout after pathogen mycelia growth, wherein trans -2- hexenes
When the concentration of aldehyde is up to 0.05 μ L/L, when the concentration of hexanal and cis -3- hexenoic aldehydes is up to 0.5 μ L/L, mycelia life can be significantly reduced
Long by 12~24%, during 10 × higher concentration, mycelial growth is almost totally constrained.
In addition, film potential has very important effect, film potential to the energetic supersession of mycelia with growing and infecting host
Largely reflect the physiological status of cell.In order to verify and probe into life of the C6 volatile Aldehyde components for the pathogen
Reason influences, and the film potential of different volatile materials treated mycelia is determined, film potential passes through after being dyed by JC1
Confocal microscopy simultaneously carries out fluorescent quantitation.
The result shows that it is corresponding with mycelial growth, with the rising of C6 volatile Aldehyde components concentration, strawberry spherical cavity bacterium
(M.fragariae) film potential of mycelia progressively declines, when the concentration of trans -2- hexenoic aldehydes is up to 0.5 μ L/L and higher, hexanal
During with the concentration of cis -3- hexenoic aldehydes up to 5 μ L/L and higher, mycelia mitochondrial membrane potential is remarkably decreased.C6 aldehydes volatility objects
Matter can destroy the film potential of the pathogen, and then influence the growth of thalline.
The present invention also provides C6 volatile Aldehyde components to prepare the prevention microbial white leaf spot of strawberry of strawberry spherical cavity
Application in preparation, the C6 volatile Aldehyde components are hexanal, trans -2- hexenoic aldehydes or cis -3- hexenoic aldehydes；With strawberry institute
Periphery closed environment stereometer, the concentration of the hexanal is 0.5~50 μ L/L, the concentration of the trans -2- hexenoic aldehydes
For 0.5~5 μ L/L, the concentration of the cis -3- hexenoic aldehydes is 0.5~50 μ L/L.
Specifically, the white leaf spot of strawberry is located on Strawberry Leaves.
The present invention also provides a kind of method for preventing the microbial white leaf spot of strawberry of strawberry spherical cavity, with C6 aldehydes volatility
Substance fumigates strawberry as fumigant；The C6 volatile Aldehyde components are hexanal, trans -2- hexenoic aldehydes or cis -3- hexenes
Aldehyde；With the stereometer of the periphery closed environment where strawberry, the concentration of the hexanal for 0.5~50 μ L/L, the trans -2- oneself
The concentration of olefine aldehydr is 0.5~5 μ L/L, and the concentration of the cis -3- hexenoic aldehydes is 0.5~50 μ L/L.
Preferably, stifling Strawberry Leaves.
Further, the concentration of the hexanal is 5 μ L/L, and the concentration of the trans -2- hexenoic aldehydes is 0.5 μ L/L, described
The concentration of cis -3- hexenoic aldehydes is 5 μ L/L.Under the concentration, C6 volatile Aldehyde components show best inhibition and
To blade without any damage.
Compared with prior art, the invention has the advantages that：
(1) present invention firstly discloses hexanal, trans -2- hexenoic aldehydes and the cis -3- hexenoic aldehydes in Strawberry Leaves this three
Kind C6 volatile Aldehyde components can inhibit strawberry spherical cavity bacterium, the especially spore germination of strawberry spherical cavity bacterium and mycelial growth, and
The experiment of strawberry spherical cavity bacterium or Strawberry Leaves is fumigated by using the C6 volatile Aldehyde components, it was demonstrated that hexanal, trans -2- oneself
The effect and prevention of olefine aldehydr and cis -3- hexenoic aldehydes in the spore germination and mycelial growth of strawberry spherical cavity bacterium is effectively inhibited
Function in white leaf spot of strawberry.
(2) present invention prepares prevention strawberry spherical cavity bacterium using hexanal, trans -2- hexenoic aldehydes and cis -3- hexenoic aldehydes and causes
White leaf spot of strawberry preparation, and carry out white leaf spot of strawberry disease control, control effect is apparent, can effectively reduce the use of pesticide
Amount, the biological control method to develop new, efficient, green safe prevention white leaf spot of strawberry provide foundation.
Description of the drawings
Fig. 1 is the form of strawberry spherical cavity bacterium (M.fragariae) under different growth conditions；
Wherein, A is not sprout spore；B is the mycelia that growth is inhibited by volatile materials；C is the bacterium after normal growth 16h
Silk；D is the mycelia sprouted by volatile materials inhibits but 72h sprouts after removal again.
Fig. 2 is influence of the hexanal to strawberry spherical cavity bacterium (M.fragariae) mycelial growth.
Fig. 3 is influence of the trans -2- hexenoic aldehydes to strawberry spherical cavity bacterium (M.fragariae) mycelial growth.
Fig. 4 is influence of the cis -3- hexenoic aldehydes to strawberry spherical cavity bacterium (M.fragariae) mycelial growth.
Fig. 5 is influence of the C6 volatile Aldehyde components to strawberry spherical cavity bacterium (M.fragariae) film potential；
Wherein, A is hexanal；B is trans -2- hexenoic aldehydes；C is cis -3- hexenoic aldehydes.
Fig. 6 is the Strawberry Leaves lesion area that C6 volatile Aldehyde components infect strawberry spherical cavity bacterium (M.fragariae)
Influence.
Specific embodiment
The measure of main volatile compounds in 1 Strawberry Leaves of embodiment
1. experiment material
Fresh strawberry blade is derived from strawberry (Fragaria × ananassa, Albion), trade name " A Er ratios ".
2. experimental method
Be enriched with using Headspace solid phase microextractiom-internal standard method for gas chromatography method (GC-MS) detected in Strawberry Leaves
Main volatile materials and its content.
Take 20g fresh strawberries blade with saturated salt solution according to (1g:2.5mL) mixing and homogenate, precise 3g samples
It is placed in the head space bottle of 15mL, adds in 1ul 300ppm, 2,6- dimethyl -6- heptene -2- alcohol are as internal standard；It is stirred at 40 DEG C
After balancing 15min, 50/30 μm of DVB/CAR/PDMS solid phase micro-extracting heads (Supelco, Bellefonte, PA, USA) extraction is inserted into
Take 30min.
After extraction, hand sampling, 250 DEG C of parsing 3min of injection port, using gassy system (6890N) series connection MS detections
Device (5975B) (Agilent, Santa Clara, CA, the U.S.) is detected.Chromatography column parameter：DB-5MS pillars (30m ×
0.25mm,0.25μm)；Column temperature program is set：40 DEG C (retaining 3min), with 2 DEG C of min-164 DEG C are risen to, with 3 DEG C of min-1On
145 DEG C are raised to, then with 15 DEG C of min-1It rises to 250 DEG C (retaining 10min).
Each volatile component passes through interior scalar quantity by being compared to differentiate with mass spectral database (NIST05).
3. interpretation of result
The main volatile compounds measured in strawberry, as shown in table 1, wherein 2- hexenoic aldehydes, hexanal, trans -2- hexenes
The C6 small molecule aldehyde volatile matter content highests such as aldehyde, cis- 3- hexenoic aldehydes, account for more than the 85% of total amount.
Main volatile compounds and its content in 1 Strawberry Leaves of table
In view of the antibacterial activity of C6 volatile Aldehyde components is it has been reported that this invention is selected in such Strawberry Leaves naturally
Existing volatile materials alternately, separately since commercially available 2- hexenoic aldehydes are all for trans -2- hexenoic aldehydes, so follow-up real
Apply case only with trans -2- hexenoic aldehydes unused 2- hexenoic aldehydes.Meanwhile with the nature of these volatile materials in Strawberry Leaves
Content is reference to set the tested concentration of follow-up subject volatile materials.
Main volatile compounds are to strawberry spherical cavity bacterium spore germination and the inhibition of mycelial growth in 2 Strawberry Leaves of embodiment
1. experiment material
1) for examination pathogen
Strawberry spherical cavity bacterium (M.fragariae) picks up from the strawberry of nature leave piece snake eye disease, by laboratory point
From after, purifying, identification and saving backup, spore used in present case is all within four generation of Strawberry Leaves medium culture.
2) reagent agent
(98%) Sigma-Aldrich, content are all higher than, cis -3- hexenoic aldehydes (Sigma- for hexanal, trans -2- hexenoic aldehydes
Aldrich, 50% is stable at glyceryl triacetate)
2. experimental method
It is 5 × 10 by concentration4Strawberry spherical cavity bacterium (M.fragariae) the spore suspension 100uL of a/mL is uniformly coated on
In PDA culture medium (the every disk of 7mL culture mediums, about 0.15cm is thick, a diameter of 90mm of culture dish), stand 10min and spore is allowed to be attached to
Media surface；
The filter paper of a 90mm is placed in culture dish cover inside, then respectively drips the volatile materials of certain volume
It is added on filter paper, lid lid is returned into culture dish rapidly, and with the closed sealing of sealed membrane.
The volume for each volatile materials being added dropwise reaches 0.05,0.5,5,50,500 μ according to concentration in final culture dish
L/L is implemented, and for low concentration therein, each volatile materials needs to carry out 1 with water according to experiment before dropwise addition:10、1:
100、1:103、1:104Corresponding volume is added dropwise after dilution again；Each volatile materials grouping is tested, and every group of setting is only dripped
Add the control group of isometric water, the volatile materials volume being added dropwise is volatilized for 16 μ L-50 μ L and all in 8h.
9 visuals field are randomly selected after 16h under an optical microscope to be taken pictures (amplification factor 125), then using aobvious
Micro mirror capture softwares are measured and count to each thalline length in the visual field；Thalline number in each visual field 6 with
On, the average thalline length for taking each visual field is parallel as one.
The measure of Hyphal length：The thalline picture of 16h captures is using the mating image measurement software of microscope to thalline axis
It is measured to total length.
The judgement of spore germination：Thalline elongation for spore average axial length (about 41 μm) be considered as more than twice sprouting (>82
μm)；All spore numbers in the spore number/visual field sprouted in spore germination rate=visual field；
For the processing group that 16h does not sprout, filter paper is removed, and culture dish is opened, the 1h that divulges information on super-clean bench allows phase
Volatile materials is answered to remove, then after seal culture 72h again, then observe spore germination situation；This is waved if spore is sprouted again
Volatile material only plays an important role of to inhibit spore germination under the concentration, is considered as the volatile materials if spore is not sprouted yet and exists
There is bactericidal effect under the concentration.
Entire experiment is independent to be repeated twice.
3. interpretation of result
The aspect graph of the strawberry spherical cavity bacterium (M.fragariae) of different growth conditions, as shown in Figure 1.
A is not sprout spore；B is the mycelia that growth is inhibited by volatile materials；C is the mycelia after normal growth 16h；D
The mycelia sprouted again by 72h after volatile materials inhibition but removal for sprouting.
Influence of the volatile materials to strawberry spherical cavity bacterium (M.fragariae) spore germination in 2 Strawberry Leaves of table
* this volatile materials has bacteriostasis under the concentration：72h spores are sprouted again after removal.
This volatile materials has bactericidal effect under the Δ concentration：72h spores are not sprouted yet after removal.
1) C6 volatile Aldehyde components hexanal is to the shadow of (M.fragariae) spore germination of strawberry spherical cavity bacterium and mycelial growth
It rings respectively as shown in table 2 and Fig. 2.
Hexanal has apparent bacteriostasis when concentration reaches 5 μ L/L, the spore germination receipts under the concentration hexanal environment
It is only 1% to inhibition；When hexanal concentrations raise again in system (50 μ L/L, 500 μ L/L), show significantly sterilization and make
With spore cannot still be sprouted after removing hexanal pressure.
In addition, in lower concentration 0.05 μ L/L and 0.5 μ L/L, hexanal also can effectively inhibit the growth of mycelia, right
The length of thalli growth 16h of should being averaged has dropped 13.5% and 19.5% respectively.
2) trans -2- hexenoic aldehydes of C6 volatile Aldehyde components are to (M.fragariae) spore germination of strawberry spherical cavity bacterium and bacterium
The influence difference of silk growth is as shown in Table 2 and Figure 3.
The volatile materials of other tests is compared, trans -2- hexenoic aldehydes show most effective inhibition, and environment is dense
Degree is in 0.5 μ L/L, it is possible to effectively inhibit the sprouting of spore, spore germination rate is only 8% or so under the concentration, and average
Hyphal length, which is substantially less than, to be compareed；Apparent bactericidal effect is shown in its higher concentration, after 5 μ L/L and concentrations above are handled
Spore no longer have sprouting ability.Meanwhile in 0.05 μ L/L of lower concentration, it is possibility to have the inhibition of effect is mycelial to stretch
Long, its growth declines 11.6%.
3) cis -3- hexenoic aldehydes of C6 volatile Aldehyde components are to (M.fragariae) spore germination of strawberry spherical cavity bacterium and bacterium
The influence of silk growth is respectively as shown in table 2 and Fig. 4.
When cis -3- hexenes aldehyde concentration reaches 0.5 μ L/L in system, there is apparent inhibitory action to mycelial growth,
Mycelial growth declines 24.5%.In higher concentration, spore germination is almost suppressed, and thalline does not extend.In system concentration
Reach 5 μ L/L and more than after, cis -3- hexenoic aldehydes show significant bactericidal effect, suitable in removal system after suffocating treatment
Formula -3- hexenoic aldehydes spore cannot still be sprouted, and comparison compares its sterilizing rate and improves more than 90%.
Influence of the embodiment 3C6 volatile Aldehyde components to strawberry spherical cavity bacterium (M.fragariae) film potential
1. experiment material
1) for examination pathogen
Strawberry spherical cavity bacterium (M.fragariae), with case study on implementation 2.
2. experimental method
1) it is 5 × 10 by concentration4Strawberry spherical cavity bacterium (M.fragariae) the spore suspension 100uL of a/mL is uniformly coated on
In PDA culture medium (the every disk of 20mL culture mediums, about 0.15cm is thick, a diameter of 90mm of culture dish), after culture 5 days, according to implementation case
Method in example 2 carries out C6 volatile Aldehyde components suffocating treatments 16h.
After treatment, the picking mycelia from each group (are given birth in the green skies using mitochondrial membrane potential detection kit (JC-1)
Object science and technology, Shanghai) mitochondrial membrane potential of mycelia is detected.
The experimental procedure that concrete operations are provided according to kit carries out, and fluorescence intensity passes through Laser Scanning Confocal Microscope SP8 (Lays
Card, Germany) it is detected.Each test each processing group in triplicate, and every group is done technology repetition three times.Final film potential knot
Fruit is with red fluorescence intensity (excitation wavelength 525nm, launch wavelength 590nm) and green fluorescence intensity (excitation wavelength 490nm, hair
The long 530nm of ejected wave) ratio be expression result as final film potential.10 μM of CCCP processing is as positive control (CCCP
Processing can cause mycelia film potential to completely lose), water process is as blank control.
3. interpretation of result
It is corresponding with mycelial growth, with processing used by C6 volatile Aldehyde components concentration rising, strawberry ball
The mitochondrial membrane potential of chamber bacterium (M.fragariae) mycelia progressively declines (Fig. 5), works as concentration up to 0.5 for trans -2- hexenoic aldehydes
μ L/L and during higher, hexanal and cis -3- hexenoic aldehydes are remarkably decreased in the film potential up to 5 μ L/L mycelia, show C6 aldehyde materials
The film potential of the pathogen can be destroyed, and then influences the growth of thalline.When the concentration of trans -2- hexenoic aldehydes reaches 50 μ L/L
When, the film potential of mycelia is almost lost, similar to 10 μM of CCCP handling results.
Embodiment 4C6 volatile Aldehyde components infect strawberry spherical cavity bacterium (M.fragariae) inhibition of Strawberry Leaves
1. experiment material
1) for examination Strawberry Leaves
Fresh A Er, with embodiment 1, chooses the similar blade of growth conditions and carries out than the fresh strawberry blade of strawberry
Experiment.
2) for examination pathogen
Strawberry spherical cavity bacterium (M.fragariae), with embodiment 2.
2. experimental method
The similar Strawberry Leaves of fresh, physiological status are placed in 90mm culture dishes, and spread two layers of filter paper in blade it
Under, 1mL water is added dropwise to filter paper, to keep the humidity of blade and intact physiological status.
It is 1 × 10 by concentration5Strawberry spherical cavity bacterium (M.fragariae) spore suspension of a/mL be sprayed on face of blade and
Reverse side carries out artificial infection；After the completion of inoculation, the filter paper of a 90mm is placed in culture dish cover inside, then respectively by one
The volatile materials for determining volume is added dropwise on filter paper, and lid lid returned culture dish rapidly, and with the closed sealing of sealed membrane.Each C6
The concentration of aldehyde material is respectively 0 (control), and 5,50,500 μ L/L are implemented.
Blade opens culture dish after handling 2h at room temperature, by with volatile materials filter paper remove, culture dish without
It is sealed again after collarium border lower open mouth 30min (fully removing volatile materials).Strawberry is observed and recorded after being further cultured for 8 days at room temperature
The incidence of blade.
3. interpretation of result
The C6 volatile Aldehyde components suffocating treatment of various concentration has the incidence of white leaf spot of strawberry the shadow of highly significant
It rings (Fig. 6).When trans -2- hexenoic aldehydes concentration for the treatment of can inhibit the morbidity of white leaf spot of strawberry for 0.5,5 μ L/L phases than control
Rate wherein 0.5 μ L/L effects are best, almost inhibits infecting for the pathogen.For hexanal and cis -3- hexenoic aldehydes,
Its optimal concentration is 5 μ L/L, in addition, 0.5 and 50 μ L/L processing also has certain inhibitory action for the generation of disease.
However, excessive concentrations volatile materials processing, Strawberry Leaves are also brought along with damage in itself, as trans -2- oneself
Olefine aldehydr is 50 μ L/L and higher in concentration, and when hexanal and cis -3- hexenes aldehyde concentration are 500 μ L/L, treated, and blade occurs
Brown shows that blade cell is damaged, and is easier to be infected be subject to pathogen so as to cause blade, and rear blade is almost complete within 8 days
It is infected by strawberry spherical cavity bacterium (M.fragariae) in portion.
According to result above preferably, hexanal, 5 μ L/L；Trans -2- hexenoic aldehydes, 0.5 μ L/L, cis -3- hexenoic aldehydes, 5
Best inhibition is shown during μ L/L and to blade without any damage.
In conclusion the main C6 volatile Aldehyde components in Strawberry Leaves, mainly including hexanal, trans -2- hexenoic aldehydes,
Cis -3- hexenoic aldehydes, under certain ambient concentration have significantly inhibit (M.fragariae) spore germination of strawberry spherical cavity bacterium and
The effect of mycelial growth, and can be so that pathogen mycelia mitochondrial membrane potential declines, so as to influence the growth of fungi.Suitable
Under concentration, C6 aldehyde materials can effectively inhibit the intrusion of the pathogen, prevent the generation of white leaf spot of strawberry, and in preferred agent
Under amount：5 μ L/L of hexanal；Best inhibition is shown during trans 0.5 μ L/L, cis 5 μ L/L of -3- hexenoic aldehydes of -2- hexenoic aldehydes
And to blade without any damage.This method to white leaf spot of strawberry it was proved that have good preventive and therapeutic effect.
It is demonstrated experimentally that hexanal, trans -2- hexenoic aldehydes and cis -3- hexenoic aldehydes have white leaf spot of strawberry prevention well
Effect.
Claims (8)
- Application of the 1.C6 volatile Aldehyde components in strawberry spherical cavity bacterium is inhibited, which is characterized in that the C6 aldehydes volatility object Matter is hexanal, trans -2- hexenoic aldehydes or cis -3- hexenoic aldehydes.
- 2. the application as described in right 1, which is characterized in that the C6 volatile Aldehyde components are used to inhibit the spore of strawberry spherical cavity bacterium Son is sprouted and mycelial growth.
- 3. the application as described in right 2, which is characterized in that in the spore germination for inhibiting strawberry spherical cavity bacterium, with strawberry spherical cavity bacterium The stereometer of the periphery closed environment at place, the μ L/L of the concentration of the hexanal >=0.5, the concentration of the trans -2- hexenoic aldehydes >= 0.05 μ L/L, the μ L/L of the concentration of the cis -3- hexenoic aldehydes >=0.5.
- 4. the application as described in right 2, which is characterized in that in the mycelial growth for inhibiting strawberry spherical cavity bacterium, with strawberry spherical cavity bacterium The stereometer of the periphery closed environment at place, the μ L/L of the concentration of the hexanal >=0.05, the concentration of the trans -2- hexenoic aldehydes >= 0.05 μ L/L, the μ L/L of the concentration of the cis -3- hexenoic aldehydes >=0.05.
- Application of the 5.C6 volatile Aldehyde components in the preparation for preparing the prevention microbial white leaf spot of strawberry of strawberry spherical cavity, it is special Sign is that the C6 volatile Aldehyde components are hexanal, trans -2- hexenoic aldehydes or cis -3- hexenoic aldehydes；With outer where strawberry The stereometer of all closed environments, the concentration of the hexanal are 0.5~50 μ L/L, the concentration of the trans -2- hexenoic aldehydes for 0.5~ 5 μ L/L, the concentration of the cis -3- hexenoic aldehydes is 0.5~50 μ L/L.
- 6. the application as described in right 5, which is characterized in that the white leaf spot of strawberry is located on Strawberry Leaves.
- A kind of 7. method for preventing the microbial white leaf spot of strawberry of strawberry spherical cavity, which is characterized in that with C6 volatile Aldehyde components As fumigant, strawberry is fumigated；The C6 volatile Aldehyde components are hexanal, trans -2- hexenoic aldehydes or cis -3- hexenoic aldehydes； With the stereometer of the periphery closed environment where strawberry, the concentration of the hexanal is 0.5~50 μ L/L, the trans -2- hexenes The concentration of aldehyde is 0.5~5 μ L/L, and the concentration of the cis -3- hexenoic aldehydes is 0.5~50 μ L/L.
- 8. the method as described in right 7, which is characterized in that the concentration of the hexanal is 5 μ L/L, the trans -2- hexenoic aldehydes Concentration is 0.5 μ L/L, and the concentration of the cis -3- hexenoic aldehydes is 5 μ L/L.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113229389A (en) * | 2021-05-28 | 2021-08-10 | 杭州国慷网络科技有限公司 | Processing and storing method of gynura procumbens pseudo-ginseng leaf powder |
| CN115812709A (en) * | 2022-09-28 | 2023-03-21 | 浙江大学 | Application of aldehyde volatile substances in inhibition of sclerotinia sclerotiorum |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104041547A (en) * | 2014-05-29 | 2014-09-17 | 赵兰 | Natural bactericide for controlling strawberry leaf spot and preparation method thereof |
| CN105010429A (en) * | 2015-06-26 | 2015-11-04 | 安徽禾众农业科技有限公司 | Strawberry white leaf spot prevention and control bacteriostat, and preparation method and application thereof |
| CN107711911A (en) * | 2017-11-09 | 2018-02-23 | 淮南市永光草莓专业合作社 | For preventing and treating the composition of white leaf spot of strawberry |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104041547A (en) * | 2014-05-29 | 2014-09-17 | 赵兰 | Natural bactericide for controlling strawberry leaf spot and preparation method thereof |
| CN105010429A (en) * | 2015-06-26 | 2015-11-04 | 安徽禾众农业科技有限公司 | Strawberry white leaf spot prevention and control bacteriostat, and preparation method and application thereof |
| CN107711911A (en) * | 2017-11-09 | 2018-02-23 | 淮南市永光草莓专业合作社 | For preventing and treating the composition of white leaf spot of strawberry |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113229389A (en) * | 2021-05-28 | 2021-08-10 | 杭州国慷网络科技有限公司 | Processing and storing method of gynura procumbens pseudo-ginseng leaf powder |
| CN115812709A (en) * | 2022-09-28 | 2023-03-21 | 浙江大学 | Application of aldehyde volatile substances in inhibition of sclerotinia sclerotiorum |
| CN115812709B (en) * | 2022-09-28 | 2024-03-29 | 浙江大学 | Application of aldehyde volatile substances in inhibiting sclerotinia sclerotiorum |
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