CN108066237A - It is a kind of that there are whitening, the composition of light spot effect and its application, skin care item - Google Patents
It is a kind of that there are whitening, the composition of light spot effect and its application, skin care item Download PDFInfo
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- CN108066237A CN108066237A CN201711476645.2A CN201711476645A CN108066237A CN 108066237 A CN108066237 A CN 108066237A CN 201711476645 A CN201711476645 A CN 201711476645A CN 108066237 A CN108066237 A CN 108066237A
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- conchiolin
- bulbus lilii
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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Abstract
The present invention provides a kind of with whitening, the composition of light spot effect and its application, skin care item.Composition provided by the invention includes：Hydrolyze conchiolin 0.01~1% and Flos Lilii viriduli extract 0.01~3%.In composition of the present invention; hydrolyze conchiolin and Flos Lilii viriduli extract coordinated; resulting composition has significantly whitening, light spot effect, while have antiallergic, anti-inflammatory efficacy, can effectively protect the skin from damage and pigmentation caused by UVB irradiations.
Description
Technical field
It is more particularly to a kind of that there is whitening, the composition of light spot effect and its application, shield the present invention relates to cosmetic field
Skin product.
Background technology
Recently as environmental pollution, rhythm of life is accelerated, night life is enriched, and more and more people are chronically at sub- strong
Health state, so as to cause the dim heavy, color spot of skin increases, aging is accelerated phenomena such as, increasingly aggravate.Everybody has a love of beauty, smooth
The skin of whitening is the target that everybody pursues, particularly female friend, and with the raising of living standard, people progressively pursue
Quality of the life pursues crude.
At present, skin care item in the market are mainly using various natural plant extracts as active ingredient.Wherein, Whitening, spot
Though skin care item species is various, effect is irregular, mostly not notable to the removal effect of melanin, and Whitening, spot effect is not
It is preferable.
The content of the invention
Present invention solves the technical problem that it is to provide a kind of with whitening, the composition of light spot effect and its application, shield
Skin product, damage caused by said composition can effectively protect the skin from UVB irradiations, have significantly whitening, light spot effect.
The invention discloses a kind of compositions with whitening, light spot effect, are carried including hydrolysis conchiolin and lily
Take object.
In embodiment provided by the invention, in terms of weight %, the composition includes：
Hydrolyze conchiolin 0.01~1.00%；
Bulbus Lilii extract 0.01~3.00%；
Water surplus.
In embodiment provided by the invention, in terms of weight %, the composition includes：
Hydrolyze conchiolin 0.03~0.50%；
Bulbus Lilii extract 0.02~2.00%；
Water surplus.
In some specific embodiments provided by the invention, the composition includes：
Hydrolyze conchiolin 0.15%；
Bulbus Lilii extract 0.85%；
Water surplus.
In some specific embodiments provided by the invention, the composition includes：
Hydrolyze conchiolin 0.01%；
Bulbus Lilii extract 3.00%；
Water surplus.
In some specific embodiments provided by the invention, the composition includes：
Hydrolyze conchiolin 1.00%；
Bulbus Lilii extract 0.01%；
Water surplus.
Preferably, the preparation method of the hydrolysis conchiolin is：
Step 1:Crushing pearl is taken, water is added to stir to get pearl slurry, adds in lactic acid, reacts 1.5h~3h in 95~100 DEG C,
Filtrate and filter residue are collected in filtering respectively；
Step 2：Filter residue is taken to add water, pH value is adjusted to 6.0~8.0, adds in neutral proteinase, 39~41 DEG C of enzyme digestion reactions 2
Filtrate is collected in~4h, filtering；
Step 3：Merge the filtrate of step 1 and step 2, mixing, centrifugation freezes, and obtains hydrolysis conchiolin.
Wherein, pH value will preferably be adjusted to 7.0, the number of the enzyme digestion reaction is preferably 2 times, and the temperature of enzyme digestion reaction is excellent
Elect 40 DEG C as, enzymolysis time is preferably 3h.
Preferably, in composition of the present invention, the preparation method of the Bulbus Lilii extract is：Using lily petal as
Explant obtains lily callus through Fiber differentiation, successively through secondary culture, Shaking culture, is scattered in after cleaning in glycerine,
Obtain Bulbus Lilii extract.Wherein, lily petal preferably uses the petal of white lily, it is preferred to use N6 culture medium Fiber differentiations obtain
Bai Baihe callus.
In some specific embodiments provided by the invention, the preparation method of the Bulbus Lilii extract is：
The lily petal after sterilizing is cut, as explant, explant is placed in Fiber differentiation in N6 solid mediums, is obtained
To lily callus；The preferable cell mass secondary culture of body is chosen from callus, so as to obtain loose particles shape
Cell mass；It is filtered, the cell mass of individual cells or peanut is filtered out as competent cell, moves to the shaking flask equipped with culture solution
Middle carry out Shaking culture, obtains free cell.Then free cell is put on gauze, is rinsed with clear water, remove culture solution
In impurity, be finally scattered in glycerine, obtain Bulbus Lilii extract.
The present invention also provides above-mentioned composition prepare with whitening, light spot effect cosmetics in application.
The present invention also provides a kind of skin care item with whitening, light spot effect, by can on above-mentioned composition and cosmetics
The auxiliary material of receiving is made.
The present invention's has whitening, the composition of light spot effect, in terms of weight %, including：Hydrolyze conchiolin 0.01
~1%；Flos Lilii viriduli extract 0.01~3%.The present invention selects Bulbus Lilii extract to be compounded with hydrolysis conchiolin, each group
Divide reasonable formula, coordinated, resulting composition can significantly reduce the expression quantity of Rab27, PMEL, KIT, so as to reduce black
The generation of element；And the content of inflammatory factor IL-1 α, PGE2 and TNF α can be significantly reduced, so as to reduce the synthesis of melanin；And
And the expression quantity of CD44 albumen is improved, damage caused by protecting the skin from UVB irradiations has significantly whitening, light spot and guarantor
Wet effect.
Specific embodiment
There is whitening, the composition of light spot effect and its application, skin care item, people in the art the invention discloses a kind of
Member can use for reference present disclosure, be suitably modified technological parameter realization.In particular, it should be pointed out that all similar substitutions and modifications
Apparent to those skilled in the art, they are considered as being included in the present invention.The method of the present invention and application
Be described by preferred embodiment, related personnel substantially can not depart from present invention, in spirit and scope it is right
Method described herein and application are modified or suitably change with combining, to realize and using the technology of the present invention.
With reference to embodiment, the present invention is further explained：
For a further understanding of the present invention, with reference to embodiment to provided by the invention with whitening, light spot effect
Composition and its application, skin care item illustrate, and protection scope of the present invention is not limited by the following examples.
The 3D skin models used in embodiment are purchased from Lab skin creations companies；Rab27 test kits
Purchased from Sigma companies.
The preparation of 1 composition of embodiment
(1) pearl taken to clean, dried, crushed 100 mesh sieves, weighing 200g pearl powders, a small amount of deionized water being added in, in water
Pearl slurry is stirred into bath, when temperature is increased to 90 DEG C, 430ml lactic acid is added in, boils, 95~100 DEG C of reaction 2h are constantly stirred
It mixes, after reaction, filtering, filtrate is individually stored, and filter residue adds appropriate amount of deionized water, with Ca (OH)2PH value is adjusted to 7.0, is added
Enter 0.2g neutral proteinases, react 3h at 40 DEG C；Then 0.2g neutral proteinases are added in, 3h, reaction knot are reacted at 40 DEG C
Shu Hou, filtering merge front and rear filtrate twice, mixing, and ultracentrifuge surpasses from rear, freezes to hydrolyze conchiolin.
(2) lily petal is taken, sterilization cuts the lily petal after sterilizing, as explant, explant is placed in N6
Fiber differentiation in solid medium obtains white lily callus；The preferable cell mass passage of body is chosen from callus
Culture obtains the cell mass of loose particles shape；Filtered, the cell mass for filtering out individual cells or peanut is thin as activity
Born of the same parents move to and Shaking culture are carried out in the shaking flask equipped with culture solution, obtain free cell.Then free cell is put into gauze
On, it is rinsed with clear water, removes the impurity in culture solution, be finally scattered in glycerine, obtain Bulbus Lilii extract.
The water mixing obtained above for hydrolyzing conchiolin 0.15%, Bulbus Lilii extract 0.85% and surplus is taken, is obtained
Composition with whitening, light spot effect.
The preparation of 2 composition of embodiment
Conchiolin 0.01%, Bulbus Lilii extract 3.00% are hydrolyzed, with embodiment 1, be prepared has preparation method
Whitening, the composition of light spot effect.
The preparation of 3 composition of embodiment
Conchiolin 0.10%, Bulbus Lilii extract 1.00% are hydrolyzed, with embodiment 1, be prepared has preparation method
Whitening, the composition of light spot effect.
The preparation of 4 composition of embodiment
Conchiolin 0.50%, Bulbus Lilii extract 2.00% are hydrolyzed, with embodiment 1, be prepared has preparation method
Whitening, the composition of light spot effect.
The preparation of 5 composition of embodiment
Conchiolin 1.00%, Bulbus Lilii extract 0.01% are hydrolyzed, with embodiment 1, be prepared has preparation method
Whitening, the composition of light spot effect.
(1) Whitening, spot, anti-aging effects test
Experiment packet is shown in Table 1, wherein, the formula of 1~2 composition of comparative example is shown in Table 1, and preparation method is the same as embodiment 1.Its
In, items test uses 3D skin models.The dosage of each group UVB radiation treatments is 100mJ/cm2, one time a day, continuous 1d.
1 experiment packet of table
Note：C groups best results (group that dosage is few, effect is good)
1st, melanin transfer GAP-associated protein GAP Rab27 expressions
The transport of melanosome be happened at in the micro-pipe of melanocyte dendron long axis parallel distribution, it is and related by two class micro-pipes
Dynamin control driving albumen and cytoplasm dynein.Show that the research of black matrix transmitter loss they rely on micro-pipe
Transhipment be two-way.The study found that Rab27a mediates Myosin-Va to tie by another connection melanoidin Avidin
It closes on melanosome, so that this way moving can terminate at the dendron periphery position rich in actin.Rab27's
It reduces so that melanosome will not be in dendron apical enrichment.
Rab27 is handled according to the experiment grouping of table 1.Exempt from by the way that people Ras GAP-associated protein GAPs Rab27A (Rab27A) is enzyme-linked
Epidemic disease determining adsorption (ELISA) kit measurement Rab27 ratios.The results are shown in Table 2.
2nd, premelanosome albumen PMEL expressions detection combination object whitening function
Premelanosome albumen PMEL is in premelanosome into the transition process of II phase melanosomes and melanosome structure fiber
It plays a significant role in the formation of matrix.
PMEL is handled according to the experiment grouping of table 1, and 3D skin models use active ingredient processing group and untreated right
PMEL is marked using immunohistochemical method according to group, to observe the expression of PMEL after active ingredient is handled.
The results are shown in Table 2.
3rd, using the anti-aging effects of the KIT methods detection present composition
The absolute quantity of the c-KIT positive cells purified in the elderly's marrow is apparently higher than youth group.By old c-KIT
When positive cell is transplanted with young subjects, the T cell of candidate stem cell and the differentiation capability of NK cells are significantly lower than youth
Donor.Therefore KIT-SCF systems play an important roll physiological aging.It is handled according to the experiment grouping of table 2,3D skins
Model is marked KIT using immunohistochemical method using active ingredient processing group and untreated control group, to see
Examine the expression of KIT after active ingredient is handled.The results are shown in Table 2.
4th, skin injury repair ability test is carried out using CD44 methods
CD44 is the key factor of skin injury reparation.It is handled according to the grouping of table 2, wherein, what each processing group added in
The effective ingredient consumption of composition is identical (1% composition active ingredient), then using immunohistochemical method to CD44 into
Line flag, the expression of CD44 the results are shown in Table shown in 2 after observation processing.
The expression analysis of table 2 Rab27, PMEL, KIT and CD44
The results show that compared with model control group B groups, C~G groups each epidermal cell length unit table after UVB irradiates
Picounit area KIT and PMEL area significantly reduces (P<0.001), Rab27 expressing quantities reduce (P<And CD44 0.001)
Positive cell number (P<0.001) dramatically increase.Wherein, the processing group C group dosages of 1 composition of embodiment are minimum, but effect and its
His embodiment has similar as a result, being therefore coefficient optimum proportion.
The above result shows that the composition of the embodiment of the present invention can significantly reduce the expression of PMEL, KIT, Rab27 albumen
Amount and the expression quantity for improving CD44, have effects that apparent Whitening, spot, anti-aging, can protect the skin from UVB irradiations
Caused damage.
(2) antiallergic, anti-inflammatory efficacy test
Grouping according to table 2 is handled, test method：Human keratinized cell (Lab skin creations) is seeded in
0.5cm2Polycarbonate filter on, using MCDB 153modified as culture medium and supplementing preparation.Have a common boundary in solution-air
The culture of 14 days by a definite date is carried out in face of cell, replaces 1 subculture within during which every 2 days.After 14 days, culture epidermis is formed.
Epidermis adds in composition after being formed, filtration sterilization after composition ratio mixing is irradiated using UVB150mJ/cm2.It incubates
After the completion of educating, culture medium is removed, using IL-1 α detection kits (Quantikine), PGE2 detection kits, TNF α detection examination
Agent box (Quantikine) is respectively measured IL-1 α, PGE2 and TNF α content, the results are shown in Table 3.
3 IL-1 α of table, PGE2 and TNF α assay
| Grouping | IL-1α(pg/ml) | PGE2(pg/ml) | TNFα(pg/ml) |
| A (blank control) | 101.3±6.2 | 120.5±8.2 | 90.4±5.2 |
| B (blank control+UVB) | 170.0±10.7 | 198.4±12.3 | 140.1±10.9 |
| C (1 composition+UVB of embodiment) | 110.0±9.3 | 133.6±9.2 | 105.8±7.3 |
| D (2 composition+UVB of embodiment) | 125.8±7.1 | 146.8±10.5 | 121.9±2.4 |
| E (3 composition+UVB of embodiment) | 115.6±3.7 | 134.9±8.4 | 117.7±8.2 |
| F (4 composition+UVB of embodiment) | 102.0±8.4 | 119.1±14.0 | 109.6±9.0 |
| G (5 composition+UVB of embodiment) | 128.4±6.2 | 144.1±11.1 | 110.2±6.5 |
| H (hydrolysis conchiolin+UVB) | 150.5±7.6 | 167.8±10.8 | 130.2±6.4 |
| I (Bulbus Lilii extract+UVB) | 148.9±5.2 | 170.3±9.9 | 132.8±5.6 |
| J (1 composition+UVB of comparative example) | 147.8±6.7 | 168.5±5.6 | 131.5±3.8 |
| K (2 composition+UVB of comparative example) | 151.0±5.9 | 165.0±7.2 | 128.1±3.3 |
By table 4 as it can be seen that IL-1 α, PGE2 and the TNF α of model control group are above blank control group, difference has conspicuousness
(P<0.01).IL-1 α, PGE2 and the TNF α of present composition C~G groups significantly lower than model control group, H, I group and J,
K groups are respectively provided with significant difference (P<0.0l).
Wherein, the processing group C group dosages of 1 composition of embodiment are minimum, but effect has similar knot with other embodiment
Fruit.Therefore it is coefficient optimum proportion.
In composition of the present invention, conchiolin and Flos Lilii viriduli extract coordinated, resulting composition tool are hydrolyzed
There are significantly whitening, light spot effect, while there is antiallergic, anti-inflammatory efficacy, caused by can effectively protecting the skin from UVB irradiations
Damage and pigmentation.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of have whitening, the composition of light spot effect, including hydrolysis conchiolin and Bulbus Lilii extract.
2. composition according to claim 1, which is characterized in that in terms of weight %, including：
Hydrolyze conchiolin 0.01~1.00%；
Bulbus Lilii extract 0.01~3.00%；
Water surplus.
3. composition according to claim 1, which is characterized in that in terms of weight %, including：
Hydrolyze conchiolin 0.03~0.50%；
Bulbus Lilii extract 0.02~2.00%；
Water surplus.
4. composition according to claim 1, which is characterized in that including：
Hydrolyze conchiolin 0.15%；
Bulbus Lilii extract 0.85%；
Water surplus.
5. composition according to claim 1, which is characterized in that including：
Hydrolyze conchiolin 0.01%；
Bulbus Lilii extract 3.00%；
Water surplus.
6. composition according to claim 1, which is characterized in that including：
Hydrolyze conchiolin 1.00%；
Bulbus Lilii extract 0.01%；
Water surplus.
7. according to the composition described in claim 1~6 any one, which is characterized in that the system of the hydrolysis conchiolin
Preparation Method is：
Step 1:Crushing pearl is taken, water is added to stir to get pearl slurry, adds in lactic acid, reacts 1.5h~3h, mistake in 95~100 DEG C
Filtrate and filter residue are collected in filter respectively；
Step 2：Filter residue is taken to add water, adjusts pH value to 6.0~8.0, adds in neutral proteinase, 39~41 DEG C of 2~4h of enzyme digestion reaction,
Filtrate is collected in filtering；
Step 3：Merge the filtrate of step 1 and step 2, mixing, centrifugation freezes, and obtains hydrolysis conchiolin.
8. according to the composition described in claim 2~6 any one, which is characterized in that the preparation side of the Bulbus Lilii extract
Method is：
Using lily petal as explant, lily callus is obtained through Fiber differentiation, successively through secondary culture, Shaking culture, clearly
It is scattered in after washing in glycerine, obtains Bulbus Lilii extract.
9. composition according to claim 8, which is characterized in that the lily petal be Madonna lily valve, the induction
The culture medium used is cultivated as N6 culture mediums.
10. composition as claimed in any one of claims 1 to 9 wherein prepare with whitening, light spot effect cosmetics in
Using.
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| CN201711476645.2A CN108066237B (en) | 2017-12-29 | 2017-12-29 | Composition with whitening and spot-fading effects, application thereof and skin care product |
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| CN201711476645.2A CN108066237B (en) | 2017-12-29 | 2017-12-29 | Composition with whitening and spot-fading effects, application thereof and skin care product |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111053729A (en) * | 2020-02-14 | 2020-04-24 | 广州巴宝莉化妆品有限公司 | Transparent hair conditioner with hair and hair scale repairing effect |
| CN111455010A (en) * | 2020-04-22 | 2020-07-28 | 广东丸美生物技术股份有限公司 | Pearl protein, preparation method and application thereof, and cosmetics containing pearl protein |
| WO2020221145A1 (en) * | 2019-04-29 | 2020-11-05 | 欧诗漫生物股份有限公司 | Pearl shell whitening factor and preparation method therefor and use thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020221145A1 (en) * | 2019-04-29 | 2020-11-05 | 欧诗漫生物股份有限公司 | Pearl shell whitening factor and preparation method therefor and use thereof |
| CN111053729A (en) * | 2020-02-14 | 2020-04-24 | 广州巴宝莉化妆品有限公司 | Transparent hair conditioner with hair and hair scale repairing effect |
| CN111053729B (en) * | 2020-02-14 | 2024-02-27 | 广州巴宝莉化妆品有限公司 | Transparent hair conditioner with hair and hair scale repairing effect |
| CN111455010A (en) * | 2020-04-22 | 2020-07-28 | 广东丸美生物技术股份有限公司 | Pearl protein, preparation method and application thereof, and cosmetics containing pearl protein |
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| Publication number | Publication date |
|---|---|
| CN108066237B (en) | 2020-04-14 |
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Inventor after: Liu Xiaoying Inventor after: Liu Guangrong Inventor after: Che Biao Inventor after: Tang Jian Inventor before: Liu Xiaoying Inventor before: Liu Guangrong Inventor before: Che Biao Inventor before: Tang Jian |