CN108048332A - A kind of green alga viable bacteria compound and its preparation method and application - Google Patents
A kind of green alga viable bacteria compound and its preparation method and application Download PDFInfo
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- CN108048332A CN108048332A CN201711426032.8A CN201711426032A CN108048332A CN 108048332 A CN108048332 A CN 108048332A CN 201711426032 A CN201711426032 A CN 201711426032A CN 108048332 A CN108048332 A CN 108048332A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a kind of green alga viable bacteria compounds and its preparation method and application.The green alga viable bacteria compound includes the raw material of following parts by weight：40 60 parts of green alga, 10 20 parts of viable bacteria, the green alga use chlorella, and the viable bacteria uses photosynthetic bacteria and bacillus polymyxa.The green alga viable bacteria compound of the present invention can be used for agriculture field, complementary using bacterium algae, more environmentally protective；With the effect for promoting crop growth, improving disease-resistant crops evil ability, improving crop yield and quality；The green alga viable bacteria compound of the present invention is used as foliar fertilizer, and crops can be made to improve 23 times of photosynthetic rates, make full use of nitrogen source and its carbon dioxide in air, more energy conservation and environmental protection；The green alga viable bacteria compound of the present invention also plays an important role of rehabilitating soil, can fix the harmful substances such as heavy metal, degrading polycyclic aromatic hydrocarbons, the effect for reducing salinity, improving soil water-reataining fertilizer-keeping ability.
Description
Technical field
The present invention relates to agricultural technology fields, are specifically a kind of green alga viable bacteria compound and its preparation method and application.
Background technology
Green alga is plant kingdom's Chlorophyta（Chlorophyta）Member, there are about 6,700 kinds of classification.It is distributed mainly in fresh water,
In the seawater and dark and damp place of land is also distributed.The photosynthetic pigments of green alga（Chlorophyll a and b, carrotene, lutein）Ratio
Example is similar to seed plant and other higher plants.Typical chlorella cell is movable or stiff.With a central vacuole,
For pigment in plastid, plastid shape is different due to species.Cell membrane is made of two layers of cellulose and pectic substance.Food is with the shape of starch
Formula is stored in the in vivo pyrenoids of matter.
Photosynthetic bacteria (abbreviation PSB) is to occur the prokaryotes with original luminous energy synthetic system on the earth earliest, is to detest
The general name of the photosynthetic bacterium of oxygen is not put under the conditions of oxygen, is widely present in global biosphere everywhere.It is supported in aquatic products
In growing, the noxious materials such as nitrite, sulfide in the water body that can degrade, realization serves as bait, purifies water, prevents disease
Disease, as functions such as feed addictives, its many characteristics make it have in Non-environmental Pollution Aquiculture and huge apply valency
Value.
Bacillus polymyxa exists with spore form, and temperature tolerance is good, stable quality, " fungus treatment ", lasting medicine；" medicine
Fertile and energy " is not only the optimal medicaments of soil-borne diseases such as prevention bacterial wilt, droop, root rot, soft rot, and has
The apparent effect for promoting growth, improving yield, improving quality；It is nuisanceless, green, organic vegetable without any poisoning and residual
The preferred microorganism formulation of dish production；It is notable to solanaceae class crop bacterial wilt preventive effect.Environmental Protection Agency (EPA) has been arranged
For can commercial one of microbe species.
At present, the situation is tense for arable soil Tiny ecosystem, and the disease resistance ability of crops is weak, seriously affects growing environment and product
Matter.The present invention by by green alga with viable bacteria is compound prepares compound, be conducive to improve the disease resistance ability of crops, improve growth
Speed and quality.
The content of the invention
It is an object of the invention to provide a kind of green alga viable bacteria compound and its preparation method and application, to solve the above-mentioned back of the body
The problem of being proposed in scape technology.
To achieve the above object, the present invention provides following technical solution：
A kind of green alga viable bacteria compound includes the raw material of following parts by weight：40-60 parts of green alga, 10-20 parts of viable bacteria, it is described green
Algae uses chlorella, and the viable bacteria uses photosynthetic bacteria and bacillus polymyxa.
As further embodiment of the present invention：The weight ratio of the photosynthetic bacteria and bacillus polymyxa is 1:（0.5-1.5）.
As further embodiment of the present invention：The cultural method of viable bacteria：Photosynthetic bacteria and bacillus polymyxa strain are distinguished
The aqueous solution of each culture medium is inoculated into, is placed in fermentation tank；Photosynthetic bacteria cultivates 24-28h at 28-30 DEG C；Bacillus polymyxa
18-20h is cultivated at 30-32 DEG C；Every milliliter of photosynthetic bacteria viable count is made to be more than 200,000,000, every milliliter of bacillus polymyxa viable count
More than 3,000,000,000；After the completion of culture, dormancy processing is carried out to viable bacteria.
A kind of preparation method of green alga viable bacteria compound, comprises the following steps：Green alga with viable bacteria is mixed, is sufficiently stirred
It is even, mixing speed 60-100r/min；Packed products.
A kind of application of green alga viable bacteria compound, the green alga viable bacteria compound are used for agriculture field.
As further embodiment of the present invention：100-300 times, which is diluted with water, using the green alga viable bacteria compound carries out farming
Object foliar spray operates, and the feed liquid that 200-500 volume multiples are diluted with water using the green alga viable bacteria compound carries out crops filling
Root operates.
Compared with prior art, the beneficial effects of the invention are as follows：
The green alga viable bacteria compound of the present invention can be used for agriculture field, complementary using bacterium algae, more environmentally protective；With promotion agriculture
Plant growth, the effect for improving disease-resistant crops evil ability, improving crop yield and quality；The green alga viable bacteria of the present invention is compound
Object is used as foliar fertilizer, and crops can be made to improve 2-3 times of photosynthetic rate, make full use of the nitrogen source and its titanium dioxide in air
Carbon, more energy conservation and environmental protection；Also there is rehabilitating soil, the harmful substances such as heavy metal, degrading polycyclic aromatic hydrocarbons can be fixed, reduced
Salinity, the effect for improving soil water-reataining fertilizer-keeping ability.
Specific embodiment
The technical solution of this patent is described in more detail With reference to embodiment.
Embodiment 1
A kind of green alga viable bacteria compound includes the raw material of following parts by weight：40 parts of green alga, 10 parts of viable bacteria, the green alga use
Chlorella, the viable bacteria use photosynthetic bacteria and bacillus polymyxa.The weight ratio of the photosynthetic bacteria and bacillus polymyxa is 1:
0.5。
The cultural method of viable bacteria：Photosynthetic bacteria and bacillus polymyxa strain are inoculated into the aqueous solution of each culture medium respectively,
It is placed in fermentation tank；Photosynthetic bacteria is cultivated for 24 hours at 28 DEG C；Bacillus polymyxa cultivates 18h at 30 DEG C；Make every milliliter of photosynthetic bacteria
Viable count is more than 200,000,000, and every milliliter of bacillus polymyxa viable count is more than 3,000,000,000；After the completion of culture, dormancy is carried out to viable bacteria
Processing.
A kind of preparation method of green alga viable bacteria compound, comprises the following steps：Green alga with viable bacteria is mixed, is sufficiently stirred
It is even, mixing speed 60r/min；Packed products.
A kind of application of green alga viable bacteria compound, the green alga viable bacteria compound are used for agriculture field.It is lived using the green alga
Bacterium compound is diluted with water 100 times of progress corps leaf surface spray operations, and 200 bodies are diluted with water using the green alga viable bacteria compound
The feed liquid of product multiple carries out crops pouring root operation.
Embodiment 2
A kind of green alga viable bacteria compound includes the raw material of following parts by weight：60 parts of green alga, 20 parts of viable bacteria, the green alga use
Chlorella, the viable bacteria use photosynthetic bacteria and bacillus polymyxa.The weight ratio of the photosynthetic bacteria and bacillus polymyxa is 1:
1.5。
The cultural method of viable bacteria：Photosynthetic bacteria and bacillus polymyxa strain are inoculated into the aqueous solution of each culture medium respectively,
It is placed in fermentation tank；Photosynthetic bacteria cultivates 28h at 30 DEG C；Bacillus polymyxa cultivates 20h at 32 DEG C；Make every milliliter of photosynthetic bacteria
Viable count is more than 200,000,000, and every milliliter of bacillus polymyxa viable count is more than 3,000,000,000；After the completion of culture, dormancy is carried out to viable bacteria
Processing.
A kind of preparation method of green alga viable bacteria compound, comprises the following steps：Green alga with viable bacteria is mixed, is sufficiently stirred
It is even, mixing speed 100r/min；Packed products.
A kind of application of green alga viable bacteria compound, the green alga viable bacteria compound are used for agriculture field.It is lived using the green alga
Bacterium compound is diluted with water 300 times of progress corps leaf surface spray operations, and 500 bodies are diluted with water using the green alga viable bacteria compound
The feed liquid of product multiple carries out crops pouring root operation.
Embodiment 3
A kind of green alga viable bacteria compound includes the raw material of following parts by weight：50 parts of green alga, 15 parts of viable bacteria, the green alga use
Chlorella, the viable bacteria use photosynthetic bacteria and bacillus polymyxa.The weight ratio of the photosynthetic bacteria and bacillus polymyxa is 1:
1。
The cultural method of viable bacteria：Photosynthetic bacteria and bacillus polymyxa strain are inoculated into the aqueous solution of each culture medium respectively,
It is placed in fermentation tank；Photosynthetic bacteria cultivates 26h at 29 DEG C；Bacillus polymyxa cultivates 19h at 31 DEG C；Make every milliliter of photosynthetic bacteria
Viable count is more than 200,000,000, and every milliliter of bacillus polymyxa viable count is more than 3,000,000,000；After the completion of culture, dormancy is carried out to viable bacteria
Processing.
A kind of preparation method of green alga viable bacteria compound, comprises the following steps：Green alga with viable bacteria is mixed, is sufficiently stirred
It is even, mixing speed 80r/min；Packed products.
A kind of application of green alga viable bacteria compound, the green alga viable bacteria compound are used for agriculture field.It is lived using the green alga
The feed liquid that bacterium compound is diluted with water 400 volume multiples carries out crops pouring root operation.
Embodiment 4
A kind of green alga viable bacteria compound includes the raw material of following parts by weight：45 parts of green alga, 12 parts of viable bacteria, the green alga use
Chlorella, the viable bacteria use photosynthetic bacteria and bacillus polymyxa.The weight ratio of the photosynthetic bacteria and bacillus polymyxa is 1:
0.8。
The cultural method of viable bacteria：Photosynthetic bacteria and bacillus polymyxa strain are inoculated into the aqueous solution of each culture medium respectively,
It is placed in fermentation tank；Photosynthetic bacteria cultivates 25h at 28 DEG C；Bacillus polymyxa cultivates 18.5h at 32 DEG C；Make every milliliter it is photosynthetic
Bacterium viable count is more than 200,000,000, and every milliliter of bacillus polymyxa viable count is more than 3,000,000,000；After the completion of culture, viable bacteria is stopped
Dormancy is handled.
A kind of preparation method of green alga viable bacteria compound, comprises the following steps：Green alga with viable bacteria is mixed, is sufficiently stirred
It is even, mixing speed 70r/min；Packed products.
A kind of application of green alga viable bacteria compound, the green alga viable bacteria compound are used for agriculture field.It is lived using the green alga
Bacterium compound is diluted with water 200 times of progress corps leaf surface spray operations, and 300 bodies are diluted with water using the green alga viable bacteria compound
The feed liquid of product multiple carries out crops pouring root operation.
Embodiment 5
A kind of green alga viable bacteria compound includes the raw material of following parts by weight：55 parts of green alga, 18 parts of viable bacteria, the green alga use
Chlorella, the viable bacteria use photosynthetic bacteria and bacillus polymyxa.The weight ratio of the photosynthetic bacteria and bacillus polymyxa is 1:
1.2。
The cultural method of viable bacteria：Photosynthetic bacteria and bacillus polymyxa strain are inoculated into the aqueous solution of each culture medium respectively,
It is placed in fermentation tank；Photosynthetic bacteria cultivates 27h at 29 DEG C；Bacillus polymyxa cultivates 19.5h at 32 DEG C；Make every milliliter it is photosynthetic
Bacterium viable count is more than 200,000,000, and every milliliter of bacillus polymyxa viable count is more than 3,000,000,000；After the completion of culture, viable bacteria is stopped
Dormancy is handled.
A kind of preparation method of green alga viable bacteria compound, comprises the following steps：Green alga with viable bacteria is mixed, is sufficiently stirred
It is even, mixing speed 90r/min；Packed products.
A kind of application of green alga viable bacteria compound, the green alga viable bacteria compound are used for agriculture field.It is lived using the green alga
Bacterium compound is diluted with water 250 times of progress corps leaf surface spray operations, and 400 bodies are diluted with water using the green alga viable bacteria compound
The feed liquid of product multiple carries out crops pouring root operation.
Embodiment 6
A kind of green alga viable bacteria compound includes the raw material of following parts by weight：58 parts of green alga, 14 parts of viable bacteria, the green alga use
Chlorella, the viable bacteria use photosynthetic bacteria and bacillus polymyxa.
As further embodiment of the present invention：The weight ratio of the photosynthetic bacteria and bacillus polymyxa is 1:0.6.
As further embodiment of the present invention：The cultural method of viable bacteria：Photosynthetic bacteria and bacillus polymyxa strain are distinguished
The aqueous solution of each culture medium is inoculated into, is placed in fermentation tank；Photosynthetic bacteria cultivates 27.5h at 30 DEG C；Bacillus polymyxa is 31
19.5h is cultivated at DEG C；Every milliliter of photosynthetic bacteria viable count is made to be more than 200,000,000, every milliliter of bacillus polymyxa viable count is more than 3,000,000,000
It is a；After the completion of culture, dormancy processing is carried out to viable bacteria.
A kind of preparation method of green alga viable bacteria compound, comprises the following steps：Green alga with viable bacteria is mixed, is sufficiently stirred
It is even, mixing speed 95r/min；Packed products.
A kind of application of green alga viable bacteria compound, the green alga viable bacteria compound are used for agriculture field.It is lived using the green alga
Bacterium compound is diluted with water 180 times of progress corps leaf surface spray operations, and 420 bodies are diluted with water using the green alga viable bacteria compound
The feed liquid of product multiple carries out crops pouring root operation.
The green alga viable bacteria compound of the present invention can be used for agriculture field, complementary using bacterium algae, more environmentally protective；With rush
Into crop growth, the effect for improving disease-resistant crops evil ability, improving crop yield and quality；Empirical tests, it is of the invention
Green alga viable bacteria compound is used as foliar fertilizer, and crops can be made to improve 2-3 times of photosynthetic rate, make full use of the nitrogen source in air
And its carbon dioxide, more energy conservation and environmental protection；Empirical tests, green alga viable bacteria compound of the invention also play an important role of rehabilitating soil,
The harmful substances such as heavy metal, degrading polycyclic aromatic hydrocarbons, the effect for reducing salinity, improving soil water-reataining fertilizer-keeping ability can be fixed.
The better embodiment of this patent is explained in detail above, but this patent is not limited to above-mentioned embodiment party
Formula, can also be on the premise of this patent objective not be departed from the knowledge that one skilled in the relevant art possesses
Various changes can be made.
Claims (6)
1. a kind of green alga viable bacteria compound, which is characterized in that include the raw material of following parts by weight：40-60 parts of green alga, viable bacteria
10-20 parts, the green alga uses chlorella, and the viable bacteria uses photosynthetic bacteria and bacillus polymyxa.
2. green alga viable bacteria compound according to claim 1, which is characterized in that the photosynthetic bacteria and bacillus polymyxa
Weight ratio is 1:（0.5-1.5）.
3. green alga viable bacteria compound according to claim 1, which is characterized in that the cultural method of viable bacteria：By photosynthetic bacteria and
Bacillus polymyxa strain is inoculated into the aqueous solution of each culture medium respectively, is placed in fermentation tank；Photosynthetic bacteria is cultivated at 28-30 DEG C
24-28h；Bacillus polymyxa cultivates 18-20h at 30-32 DEG C；Every milliliter of photosynthetic bacteria viable count is made to be more than 200,000,000, every milliliter
Bacillus polymyxa viable count is more than 3,000,000,000；After the completion of culture, dormancy processing is carried out to viable bacteria.
4. a kind of preparation method according to any green alga viable bacteria compounds of claim 1-3, which is characterized in that including with
Lower step：Green alga with viable bacteria is mixed, is stirred, mixing speed 60-100r/min；Packed products.
5. a kind of application according to any green alga viable bacteria compounds of claim 1-3, which is characterized in that the green alga lives
Bacterium compound is used for agriculture field.
6. the application of green alga viable bacteria compound according to claim 4, which is characterized in that using the green alga viable bacteria compound
It is diluted with water 100-300 times and carries out corps leaf surface spray operation, 200-500 bodies are diluted with water using the green alga viable bacteria compound
The feed liquid of product multiple carries out crops pouring root operation.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006432A (en) * | 2018-08-01 | 2018-12-18 | 李慧 | Application of the chlorella in cultivation technique without soil |
| CN109729803A (en) * | 2018-12-28 | 2019-05-10 | 广西壮族自治区林业科学研究院 | Balance the topdressing method of bamboo root region soil nutrient |
| CN110330983A (en) * | 2019-08-21 | 2019-10-15 | 阿尔格生命科学(江苏)有限公司 | A kind of active high-effect heavy metal degradation agent of algae |
| WO2020107116A1 (en) | 2018-11-28 | 2020-06-04 | Algueria Inc. | Process for the manufacture of a biostimulant or natural fertilizer based on fermented macroalgae, natural additives and probiotic bacteria for agriculture and horticulture |
| CN112741114A (en) * | 2021-02-23 | 2021-05-04 | 广西壮族自治区农业科学院 | Method for preventing and treating root rot of muskmelon |
| CN114107126A (en) * | 2021-12-14 | 2022-03-01 | 上海荣吉明微生物工程有限公司 | Composite microbial inoculum for degrading pesticide residues and preparation method and application thereof |
| CN114195598A (en) * | 2021-12-29 | 2022-03-18 | 河北省农林科学院农业资源环境研究所 | Microalgae fertilizer for improving soil fertility and preparation method and application thereof |
-
2017
- 2017-12-26 CN CN201711426032.8A patent/CN108048332A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006432A (en) * | 2018-08-01 | 2018-12-18 | 李慧 | Application of the chlorella in cultivation technique without soil |
| WO2020107116A1 (en) | 2018-11-28 | 2020-06-04 | Algueria Inc. | Process for the manufacture of a biostimulant or natural fertilizer based on fermented macroalgae, natural additives and probiotic bacteria for agriculture and horticulture |
| EP3887343A4 (en) * | 2018-11-28 | 2022-08-10 | Produits Bio Sun Inc. | Process for the manufacture of a biostimulant or natural fertilizer based on fermented macroalgae, natural additives and probiotic bacteria for agriculture and horticulture |
| CN109729803A (en) * | 2018-12-28 | 2019-05-10 | 广西壮族自治区林业科学研究院 | Balance the topdressing method of bamboo root region soil nutrient |
| CN110330983A (en) * | 2019-08-21 | 2019-10-15 | 阿尔格生命科学(江苏)有限公司 | A kind of active high-effect heavy metal degradation agent of algae |
| CN112741114A (en) * | 2021-02-23 | 2021-05-04 | 广西壮族自治区农业科学院 | Method for preventing and treating root rot of muskmelon |
| CN112741114B (en) * | 2021-02-23 | 2022-07-01 | 广西壮族自治区农业科学院 | Method for preventing and treating root rot of melons |
| CN114107126A (en) * | 2021-12-14 | 2022-03-01 | 上海荣吉明微生物工程有限公司 | Composite microbial inoculum for degrading pesticide residues and preparation method and application thereof |
| CN114195598A (en) * | 2021-12-29 | 2022-03-18 | 河北省农林科学院农业资源环境研究所 | Microalgae fertilizer for improving soil fertility and preparation method and application thereof |
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Application publication date: 20180518 |