CN1080474A - The combination in the control termite of chemicals and microorganism is used - Google Patents
The combination in the control termite of chemicals and microorganism is used Download PDFInfo
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Abstract
A kind of by going out except that the composition of termite that the following component of effective dose is formed: (1) is selected from the chemical termiticide of pyrethroid, pyrazolines, nitrofuanidine and nitromethane compounds, (2) predacious fungi or bacterium preferably are selected from the fungi that Conidiobolus belongs to, Paecilomyces belongs to, Veauveria belongs to or Metarhizium belongs to.Chemical termiticide is applied to the place that the place of observing termite or suspection have termite.If had described fungi or bacterium, then needn't more described fungi or bacterium be applied to this place in the place of observing termite.
Description
The present invention relates to a kind of going out except that the method and the composition that is used for this eliminating method that goes out of termite.
Known have many chemicals can kill termite under particular concentration, the instantiation of such chemicals comprises that cyfluthrin(is disclosed in for example United States Patent (USP) 4,218, in 469), unden (is disclosed in for example United States Patent (USP) 3,111, in 539), nitrile chlorobenzene phenothrin (is disclosed in for example United States Patent (USP) 4,061, in 664), isofenphos (for example be disclosed in United States Patent (USP) 3,621,082 in), Fenvalerate (is disclosed in for example United States Patent (USP) 4,024, in 163) and 1-(2-chloro-5-pyridylmethyl)-2-(nitro imino group) imidazolidine (for example be disclosed in United States Patent (USP) 4,742,060 in).
Known certain micro-organisms, particularly some predacious fungis and bacterium, the relevant adverse effect that also causes under certain conditions with the termite colony.Referring to, for example, Ko, W.H., et al, " The Nature of Soil Pernicious to Coptotermes Formosanus ", Journal of Invertebrate Pathology, the 39th volume, 38-40 page or leaf (1982).
But various known termiticides, bacterium and fungi all have some characteristics, and these characteristics make it industrial undesirable.For example, many known chemicals and microorganism must be used with very big rate of application, so that uneconomical, also be that environment is unallowed.Also usually effect is too slow for known termiticide, can not guarantee its successfully application in practice.The effect of many known termiticides depends on the specific environment of using them, and therefore many uncontrollable factors may have a negative impact to it.
Have now found that, with the chemicals of specific type be selected from the fungi of Special Category or bacterium combines when being used for handling the place that termite haunts, produced the synergy of beyond thought control termite.When being used in combination, the rate of application when rate of application is significantly less than chemical termiticide and each independent use of microorganism.The chemicals of combination of the present invention and fungi or bacterium act on a couple of days rather than in several weeks as seen.This combination make people may realize stronger, have more meaning and the control of more economical termite.
Advantageous particularly part of the present invention is that it does not use chlorinated hydrocarbon, and the latter is the one group of chemicals of controlling termite that uses the most widely.The present invention control during termite based on a kind of so new notion, promptly be used in combination the residual action chemical reagent littler, and use and handle the haunt application technique in place of termite that drug effect reduces again than known termiticide with biological agent.
An object of the present invention is to provide the bond of chemical reagent and biological substance, this bond can go out except that termite effectively.
Another object of the present invention provides a kind of composition, the environmental problem that there is not chlorinated hydrocarbon in said composition and is caused, and do not need a large amount of uses just can be effective.
A further object of the invention provides a kind of method of controlling termite effectively.
These and other conspicuous purposes concerning the professional of this area realize by a kind of composition, described composition comprises chemicals that is selected from nitrofuanidine, nitromethane class, pyrazolines and pyrethroid of (1) effective dose and (2a) predacious fungi, the fungi of preferred (Conidiobolus(Entomophthora) or Metarhizium genus or Paecilomyces or Beauveria or Actinomucor kind or (2b) carnivorism bacterium such as Serratia.The consumption of chemicals should make it be 1ppm for 0.01ppm at least or in bait usually in handled medium (as soil).The consumption of fungi or bacterium should make usually when contacting with termite, has 1-100 spore at least in the handled medium of every gram.The optimised quantity of fungi or bacterium depends on used concrete fungi or the kind of bacterium.This processing method can be to use with the same way as that is used to use other known chemical termiticides.Composition of the present invention also can be used for the bait formula neutralization and is used for handling the place of having handled again.
The present invention relates to the termiticide be made up of following component, described component is (1) at least a chemicals that is selected from following material: (a) nitrofuanidine such as 1-(2-chloro-5-pyridylmethyl)-2-(nitro imino group) imidazolidine; (b) nitromethane class such as 1-(2-chloro-5-pyridylmethyl)-the 2-(Nitromethylene) imidazolidine; (c) pyrazolines; (d) pyrethroid such as cyfluthrin, and or (2a) predacious fungi such as Conidiobolus(Entomophthora) or the fungi that belongs to of Matarhizium or Paecilomyces or Beauveria or Actinomucor or (2b) carnivorism bacterium such as Serratia.
The termiticide chemicals that is used for the present invention's practice is a known substance, can be by any known technology preparation.Some concrete nitrofuanidines and nitromethane compounds and preparation method thereof are for example disclosed in following disclosed application and patent:
EP 464，830;EP 428，541;EP 425，978;DE 36 39 877;DE 37 12 307;US 5，034，524;EP 386，565;EP 383，091;EP 375，907;EP 364，844;JP 02.207 083;EP 315，826;EP 259，738;EP 254，859;JP 63 307，857;JP 63 287，764;EP235，725;EP 212，600;EP 192，060;EP 163，855;EP154，178;EP 136，636;US 4，948，798;EP 303，570;EP 302，833;US 4，918，086;EP 306，696;FR 2，611，114;EP 183，972;EP 455，000;JP A3 279，359;JP A3，246，283;WO91/17，650;WO 91/104，965;US 5，039，686;EP 135，956;US 5，034，404;EP 471，372;EP 302，389;JP 3，220，176;Brazil 8，803，621;JP 3，246，283;JP A92/9371;and JP 3，255，072.
For example, United States Patent (USP) 4,742,060 discloses, 1-(2-chloro-5-pyridylmethyl)-2-(nitro imino group) imidazolidine can be by at room temperature by N-(2-chloro-5-pyridylmethyl) toluene solution and the cyanogen bromide reaction of ethylenediamine prepare.Make the 1-(2-chloro-5-pyridylmethyl of formation like this)-2-imido imidazolyl alkane hydrobromate again with the reaction of sulfuric acid and fuming nitric aicd.Remove dichloromethane solvent, reclaim desired 1-(2-chloro-5-pyridylmethyl)-2-(nitro imino group) imidazolidine.
Be applicable to that the chemical termiticide of the present invention in putting into practice represent with following formula:
In the formula, R represents acyl group, the alkyl of hydrogen, acyl group, replacement, alkyl, aryl, aryl, the heterocyclic radical of replacement or the heterocyclic radical that replaces of replacement;
A representative or (1) are selected from the monofunctional group of following groups: the aryl of the acyl group of hydrogen, acyl group, alkyl, aryl, replacement, the alkyl of replacement, replacement, perhaps (2) double functional group of being connected with Z;
E represents electron withdraw group;
X represents N or CH; And
Z representative or (1) are selected from the monofunctional group of following groups: the heterocyclic radical of the aryl of the alkyl of the acyl group of hydrogen, acyl group, replacement, alkyl, replacement, aryl, replacement, heterocyclic radical, replacement, OR, NR
2, SR, perhaps (2) double functional group of being connected with A or X.
The acyl group of preferred acyl group and replacement comprises the aryl phosphoryl of alkyl phosphoryl, aryl phosphoryl and replacement of aryl sulfonyl, alkyl phosphoryl, the replacement of alkyl sulphonyl, aryl sulfonyl, the replacement of aryl carbonyl, alkyl sulphonyl, the replacement of alkyl-carbonyl, aryl carbonyl, the replacement of alkyl-carbonyl, replacement.
Preferred alkyl substituent comprises: any C-C alkyl that replaces, the particularly C that replaces arbitrarily
1-C
4Alkyl, most preferably methyl, ethyl, n-pro-pyl, isopropyl, normal-butyl, sec-butyl or the tert-butyl group.
Preferred aryl groups comprises: phenyl and naphthyl, the most preferably phenyl that can replace arbitrarily.
Preferred heterocyclic radical comprises: have maximum 10 atoms wherein to have particularly N of N, an O or S(at least on the ring) aromatic ring.Particularly preferred heterocycle is thienyl, furyl, thiazolyl, imidazole radicals, pyridine radicals and benzothiazolyl.
For above-mentioned acyl group, alkyl, aryl and heterocyclic radical, suitable substituents comprises: alkyl such as methyl, ethyl, n-pro-pyl, isopropyl, normal-butyl, isobutyl group and the tert-butyl group with 1-4, preferred 1 or 2 carbon atom; Alkoxyl such as methoxyl group, ethyoxyl, positive propoxy, isopropoxy, n-butoxy, isobutoxy and tert-butoxy with 1-6, preferred 1 or 2 carbon atom; Alkylthio group such as methyl mercapto, ethylmercapto group, positive rosickyite base, different rosickyite base, positive butylthio, isobutyl sulfenyl and uncle's butylthio with 1-4, preferred 1 or 2 carbon atom; Have 1-4, preferred 1 or 2 carbon atom and 1-5, the fontanel substituted alkyl of preferred 1-3 fontanelle atom, fontanelle atom wherein can be identical or different, particularly F, Cl or Br(fluorine most preferably), trifluoromethyl for example; Hydroxyl; Fontanel is plain as fluorine, chlorine, bromine and iodine, particularly fluorine, chlorine and bromine; Cyano group; Nitro; Amino; The alkyl monosubstituted amino and the dialkyl amido that contain 1-4, preferred 1 or 2 carbon atom in the alkyl, as methylamino, methyl-ethyl-amino, n-propylamine base, isopropylamino and methyl-normal-butyl-amino; Carboxyl; Alkoxy carbonyl group such as methoxycarbonyl group and carbethoxyl group with 2-4, preferred 2 or 3 carbon atoms; Sulfo group (SO
3H-); Alkyl sulphonyl such as mesyl and ethylsulfonyl with 1-4, preferred 1 or 2 carbon atom; And the aryl sulfonyl such as the benzenesulfonyl that on aromatic ring, have 6-10 carbon atom.
In the formula I, A and Z can form together have 5-7 in the ring, the saturated or unsaturated heterocycle of preferred 5 or 6 atoms.This ring can contain 1 or 2 hetero atom such as O, S, N or N-alkyl.If in the ring two atoms being arranged is not carbon, these two atoms can identical (as two N) or difference (as a N, an O).
The instantiation of the electron withdraw group of E representative comprises NO, CN and fontanel substituted alkyl carbonyl as 1 in the formula I, 5-fontanel generation-C
1-C
6Alkyl-carbonyl.
In the formula I, Z and X can form together 5-7 in the ring, the heterocycle of preferred 5 or 6 atoms, this ring can contain 1 or 2 heteroatom such as O, S, N or N-alkyl, and heteroatom can be identical or different.The instantiation of preferred heterocycle comprises pyrrolidines, piperidines, piperazine, hexamethylene imine, morpholine and N methyl piperazine.
Preferred compound is following compound in the formula I scope, wherein
A and Z respectively represent hydrogen, can substituted alkyl, can substituted acyl group, can substituted phosphate or NR ' R " group; wherein R ' and R " respectively represent hydrogen or alkyl or 5 or 6 yuan of rings replacing arbitrarily of representative together; this ring can contain N or S as one of its ring person
E represents electron withdraw group such as NO
2, CN or COCF
3,
X represents N or CH, and
R represent hydrogen, can substituted pyridine radicals, pyridyl alkyl, thiazolyl alkyl, alkylthio alkyl or 5 or 6 yuan of rings, this ring contains O, N or S as one of its ring person, this ring can be replaced by one or more fontanel elements, alkyl, fontanel substituted alkyl or nitro.
Particularly preferred compound comprises following formula I compound, wherein,
Z represents NH, CH or NR ' R ", wherein R ' and R " is alkyl one of at least,
A represents alkyl,
A and Z represent nitrogenous 5 or 6 yuan of sulfur-bearing 5 or the 6 yuan of rings that encircle or replaced by alkylthio arbitrarily that replaced by alkylthio arbitrarily together,
E represents CN or NO
2,
X represents N or CH, and
R represents 5 or 6 yuan of rings, and this ring contains O, N or S as its at least one ring person, and this ring is at random replaced by fontanel element or alkyl; With the compound of formula II representative,
In the formula,
N represents 1 or 2,
Above the Y representative as suitable substituents to the described any substituting group of the acyl group in the formula I, alkyl, aryl and heterocyclic radical, be preferably the fontanel element, chlorine most preferably,
A, Z, X and E have the meaning in the formula I, and formula (III) compound:
A, Z, X, E, Y and n have the meaning in formula I and II in the formula.
The instantiation that can be used for nitrofuanidine of the present invention and nitromethane compounds comprises:
3-(2-chloro-5-pyridylmethyl)-and 2-(nitro imino group) thiazolidine;
1-(2-chloro-5-pyridylmethyl)-and 2-(nitro imino group) imidazolidine;
1-(2-chloro-5-pyridylmethyl)-and the 2-(Nitromethylene) imidazolidine;
R represents the aryl of alkyl, aryl or the replacement of hydrogen, alkyl, replacement in the formula,
Particularly preferred nitrofuanidine and nitromethane compounds are 1-(2-chloro-5-pyridylmethyls)-2-(nitro imino group) imidazolidine.
When using with the form of bait, the consumption of nitroguanidine and nitromethane compounds should account for the weight of 0.0001%(at least of total bait component usually), be preferably about 0.001～10.0%(weight), most preferably be about 0.01～1.0%(weight).When nitroguanidine or nitromethane compounds directly being mixed soil or directly are applied to handled surface, its consumption should make it contain at least 0.01 ppm(1,000,000 umbers in soil or on the handled surface usually), preferred about 0.1～1000ppm most preferably is about 1～300ppm.
The pyrethroid that can be used in the present composition is known.Naturally occurring and synthetic chrysanthemum ester all is suitable for.The example of suitable pyrethroid comprises: cinerins, the cinerin class, the jasmolin class, allethrin [2-methyl-4-oxo-3-(2-acrylic)-2-cyclopentene-1-base-2,2-dimethyl-3-(2-methyl isophthalic acid-acrylic) cyclopropanecarboxylcompound], dextrorotation counter-propylene chrysanthemum ester (D-trans-allethrin), barthrin [6-chloro-1,3-Ben Bing Er Evil penta ring-5-yl) methyl 2,2-dimethyl-3-(2-methyl isophthalic acid-acrylic) cyclopropanecarboxylcompound], tetramethyl chrysanthemum ester [(1,3,4,5,6,7-six hydrogen-1,3-dioxo-2H-iso-indoles-2-yl) methyl 2,2-dimethyl-3-(2-methyl isophthalic acid-acrylic) cyclopropanecarboxylcompound], alkynes sugar chrysanthemum ester [[the 5-(2-propinyl)-the 2-furyl] methyl 2,2-dimethyl-3-(2-methyl isophthalic acid-acrylic) cyclopropane carboxylic acid ester ester], resmethrin [[the 5-(phenyl methyl)-the 3-furyl] methyl 2,2-dimethyl-3-(2-methyl isophthalic acid-acrylic) cyclopropanecarboxylcompound], the bioethanomethrin[[5-(phenyl methyl)-and the 3-furyl] methyl 3-(ring pentylidene methyl)-2,2-dimethyl cyclopropane carboxylic acid ester], phenothrin [(3-Phenoxyphenyl) methyl 2,2-dimethyl-3-(2-methyl isophthalic acid-acrylic) cyclopropanecarboxylcompound], fenpropanate[cyano group (3-Phenoxyphenyl) methyl 2,2,3,3-4-methyl cyclopropane carboxylic acid ester], Permanone [(3-Phenoxyphenyl) methyl 3-(2, the 2-dichloroethylene)-2,2-dimethyl cyclopropane carboxylic acid ester], cyfluthrin[cyano group (4-fluoro-3-Phenoxyphenyl) methyl-3-(2, the 2-dichloroethylene)-2,2-dimethyl cyclopropane carboxylic acid ester], Fenvalerate [cyano group (3-epoxy and phenyl) methyl 3-(2, the 2-dichloroethylene)-2,2-dimethyl cyclopropane carboxylic acid ester] decamethrin[cyano group (3-Phenoxyphenyl) methyl 3-(2, the 2-dibromo vinyl)-2,2-dimethyl cyclopropane carboxylic acid ester], nitrile chlorobenzene phenothrin [cyano group (3-Phenoxyphenyl) methyl 4-chloro-α-(1-Methylethyl) phenylacetate, with cyhalothrin[cyano group (3-Phenoxyphenyl) methyl 3-(2-chloro-3,3,3-three fluoro-1-acrylic)-2,2-dimethyl cyclopropane carboxylic acid ester].Particularly preferred pyrethroid is cyfluthrin and nitrile chlorobenzene phenothrin.
The consumption of pyrethroid in the bait composition should use it to account for the weight of 0.0001%(at least of bait composition weight usually), preferred about 0.001-10.0%(weight), most preferably be about 0.01～1.0%(weight).When pyrethroid directly is added in the soil or directly is applied to handled when surface, its consumption should make it have preferred about 0.1～1000ppm, most preferably about 1.0～300ppm with the concentration of 0.01ppm at least usually.
Also can use any known pyrazoline compounds of the present invention in going out except that the termite composition.Such pyrazoline compounds is disclosed in for example disclosed european patent application 0,438,690.The example of suitable pyrazoline compounds comprises those pyrazoline compounds of following various representative:
α represents fontanelle atom, alkyl, fontanel element or nitro in the formula.
The consumption of pyrazoline in the bait composition should make it account for the weight of 0.0001%(at least of bait composition weight usually), preferred about 0.001-10.0%(weight), most preferably be about 0.01～1.0%(weight).When pyrazoline directly is added in the soil or directly is applied to handled when surface, its consumption should make it have preferred about 0.1～1000ppm, most preferably about 1.0～300ppm with the concentration of 0.01ppm at least usually.
Of the present invention go out except that the termite composition in used natural being present in the soil of fungi, and can be at an easy rate from wherein separating.Can be used for the Conidiobolus(Entomophthora in the termiticide of the present invention) belong to fungi and comprise Conidiobolus coronatus, Conidiobolus virulenta and Conidiobolus obscura.Particularly preferably be Conidiobolus coronatus.
Can be used for Metarhizium of the present invention and belong to that fungi is natural to be present in the soil, and can be at an easy rate from wherein separating.Various Metarhizium anisopliae bacterial strains all can be used for the present invention.Most preferably (BIO 1020 for the F52 bacterial strain of Metarhizium anisopliae, DSM Number 3884) and MADA bacterial strain (deriving from the University of Florida) (CBS Num ber 326, Baarn, Netherlands).
Can be used for Paecilomyces in the termiticide of the present invention and belong to fungi and comprise Paecilomyces farinosus, this fungi is natural to be present in the soil and can easily to separate from soil or from the back and forming the termite of spore of causing a disease by the known method of this area professional.
It is Beauveria bassiana that preferred Beauveria belongs to fungi, its also natural being present in the soil, and can easily from soil or the termite after causing a disease and that form spore, separate by the known method of this area professional.
The Actinomucor fungi also can be used in the practice of the present invention.
Can be used for bacterium of the present invention and comprise the Serratia bacterial classification, these bacterial classifications are natural to be present in the soil, and can easily separate from soil or from the back and forming the termite of sporozoite of causing a disease by the known method of this area professional.
Content of described fungi or bacterium and existence form should make in every gram medium usually at least 10
1-10
2Individual spore, preferred 10
3-10
5Individual spore.Certainly optimised quantity depends on used bacterial classification.
Termiticide of the present invention can use with following form: natural and synthetic that powder, solution, suspension, emulsion, foam, cream, particle, aerosol, usefulness reactive compound and fungi were handled and the tiny capsule in polymer.When with powder or particle form, fungi can be added in soil-nitroguanidine, nitromethane, pyrazoline or the pyrethroid Recipe with suitable amount.Can comprise at random that in final composition other additives known of using for termiticide are as expanding agent, attractant, the analeptic of looking for food, pheromones.The example of suitable dust carrier comprises clay, talcum, lime and pyrophyllite.
Termiticide of the present invention also can use with various liquid forms.The suitable liquid-carrier of using for termiticide comprises water and atent solvent.Usually other additives such as the emulsifier that can be used in the insecticidal liquid agent formulation also can be included in the liquid formulations of termiticides of the present invention.The liquid preparation of chemicals and/or fungi preparation also can comprise lignin, hydrocellulose, bentonite, pectin or use after preparation is solidified any other material.
Chemical compound and fungi or bacterium can be applied in the medium successively.When this technology of use, can apply chemical compound earlier, also can apply fungi earlier.The time interval that applies chemical compound and fungi can be as short as a few minutes, also is a couple of days even several weeks.If suitable fungi or bacteria culture have been present in the medium to be processed as the naturally occurring material with essential spore density, then do not need to add fungi or bacterium, only need add chemical compound.These processing also can be carried out to guarantee long-acting with well-regulated or random intermittence repeatedly.
Termiticide of the present invention can resist all types of termites effectively, and has found that they are effective especially to underground cave termite Reticulitermes flavipes and formosan termite Coptotermes formosanus.
Below just understand beat all synergy in conjunction with the given tables of data of the effect of using chemicals and biological agent.If these medicaments are used to separately go out except that termite, reach and use identical the going out that is obtained by combination of the present invention and remove the termite effect, then their rate of application wants several powers of high ten doubly.
The present invention described above, the following example is used for illustrating the present invention.Except as otherwise noted, otherwise all umbers that provide in these embodiments and percentage all are parts by weight and weight percent.
Embodiment
Embodiment 1-5 explanation with the chemical termiticide of independent usefulness or go out with fungi separately and compare except that termite, reaches high termite at short notice with chemical termiticide binding to fungal and goes out except that degree.The interaction of embodiment 6 and 7 explanation Conidiobolus coronatus and the agent of various pest control with insecticide ant in going out except that termite.Embodiment 8,9 and 10 explanation 1-(2-chloro-5-pyridylmethyls)-2-(nitro imino group) imidazolidine belongs to Metarhizium and the interaction of two fungal pathogens of Conidiobolus genus.Embodiment 11 explanation 1-(2-chloro-5-pyridylmethyls)-and 2-(nitro imino group) synergy of imidazolidine and three kinds of other fungies and a kind of bacterium.Embodiment 12 explanation in bacterium soil is arranged microorganism and the synergy between various nitromethane class and the nitrofuanidine.
Embodiment 1
In described each sample of table 1, use the 100g sterile soil.Control sample A does not handle with nitroguanidine, nitromethane, pyrethroid, pyrazoline or fungi.With 1-(2-chloro-5-pyridylmethyl)-2-(nitro imino group) imidazolidine directly adds in the soil among sample B, C, D, E and the F with various concentration (shown in the table 1).In the soil of these samples, do not add fungi.To have the 1-(2-chloro-5-pyridylmethyl of concentration shown in the table 1)-2-(nitro imino group) filter paper disk that soaked in the solution of imidazolidine is placed on above the soil among sample G, H and the I.In the soil of sample G, H or I, there is not fungi.In the soil of sample J, do not add nitroguanidine, but sample J comprises the soil that is obtained by used container in the previous test that wherein has fungi Conidiobolus coronatus naturally, in soil pattern K, use the soil of the previous test that wherein has fungi Conidiobolus coronatus naturally, but in addition, will add to termite with the filter paper that 0.01% nitroguanidine soaks.
Then, 1g subteranean termites Reticulitermes flavipes is added in each of soil pattern A-K.Observed result in 18 days is listed in the table 1 afterwards.
Can obviously see by the data in the table 1,, in soil, must have the 1ppm nitroguanidine in order to reach obvious effect and last (after exposing 18 days) 100% lethality to termite.(nitroguanidine as 10～100ppm) can obviously not change this result to use higher proportion in soil.
When using nitroguanidine with the form (promptly on the filter paper of handling) of poison bait, more a spot of nitroguanidine (promptly 0.001%; 0.003% and 0.010%) has same effect with the big consumption that is used to handle soil.
Data in the table 1 show that also when only applying fungi (promptly not using nitroguanidine) in soil, the gained result is the same with the result who observes in undressed control sample.That is, observe strong tunnel-effect and location in sample container bottom.Yet when the filter paper disk of existing 0.01% nitroguanidine was placed on the soil that has fungi on it, the termite of 100% in the sample died in two days.
Embodiment 2
Repeat the method for embodiment 1, but the soil pattern that uses the sterile soil sample and wherein be added with Conidiobolus coronatus with the form of a thick spore suspension.This thick spore suspension is to make by the spore with a Petri dish medium of 50cc rinsed with sterile water.With 1-(2-chloro-5-pyridylmethyl)-2-(nitro imino group) imidazolidine directly is added in the soil with various concentration (shown in the table 2).The results are shown in the table 2 of these tests.
Data in the table 2 show, at first observe the variation of termite situation in the sterile soil sample when 1ppm nitroguanidine content.Yet the nitroguanidine that needs 100ppm could produce suitable high mortality after 6 days.Have in the soil of fungi in transplanting, when 1ppm content, reached quite high lethality, and when 3ppm, reach whole lethality of termite.If do not handle, then transplant the termite of activity in undressed control sample of the termite in the soil that fungi is arranged with nitroguanidine.
Embodiment 3
In described each sample of table 3, use 100g soil.The soil pattern of half is made with sterile soil.Second half is to be made by the sterile soil of the thick spore suspension of transplanting one/sample Conidiobolus coronatus.Thick spore suspension is by accompanying the spore of Ti Shi medium to make with the 50cc rinsed with sterile water.The filter filter disc is with 1-(2-chloro-5-pyridylmethyl with concentration shown in the table 3)-2-(nitro imino group) imidazolidine is saturated.1g subteranean termites Reticulitermes flavipes is directly added each sample.Then the termite in each sample was observed 6 days.The termite lethality of each sample is listed in the table 3.
The result: if do not use any nitroguanidine, the viewing duration at 6 days, termite has the soil of Conidiobolus coronatus without any reaction to transplanting.When the filter paper poison bait that will handle is added in the soil that does not have fungi, first disease million that behavior changes appears in 0.001% handles, but even when 100 times of high degree (0.1%), lethality still very low (8%).Yet, have in the soil of fungi in transplanting, when 0.001% nitroguanidine content, the lethality after 6 days is 95%.
Table 3
The lethality (%) of nitroguanidine processing (%) termite after 6 days of filter paper disk poison bait
OF FILTER DISC BAITS transplants Conidiobolus
The soil of IN% Cor. in sterile soil
0 0 0
0.00001 0 0
0.00003 0
0.0001 0 0(+)
0.0003 50+
0.001 2+ 95+++
0.003 100
0.01 5++ 100
0.1 8+++ 100
Some reduction of (+) activity
+ many termites are got lost
++ most of termite is subjected to grievous injury
+++all termites are subjected to grievous injury
Embodiment 4
There is or does not exist 1-(2-chloro-5-pyridylmethyl in research)-2-(nitro imino group) termite directly is exposed under the situation of imidazolidine the effect of the fungi of automatic growth.In these experiments, with an agar plate that does not have fungi and do not have nitroguanidine thing as a comparison.Sample is to have the pyridylmethyl with 1-(2-chloro-5-)-2-(nitro imino group) agar plate (0.01% active component) of the filter paper disk handled of imidazolidine.The 3rd agar plate transplanted to be had Conidiobolus coronatus and uses the filter paper plate that contains 0.01% nitroguanidine to handle.Be added to termite (Reticulitermes flavipes) on each agar plate and observed 4 days.The results are shown in the table 4 of these tests.
In the control sample of not transplanting, the normal phenomenon that termite expands the tunnel immediately takes place.Do not observe lethality after 4 days.In the plate of only handling, observe 25% lethality after 4 days with fungi.In the plate of only handling with the nitroguanidine poison bait, termite seriously poisons, and does not exist with the road.For the agar of handling with fungi and nitroguanidine, 100% lethality in 1 day, occurs, and, a large amount of sporulations in 4 days, occurs for the termite of death.
Embodiment 5
4 kilograms of sands are sterilized with autoclave.Soil with sterilization is divided into two equal portions then.In a soil, water content is transferred to 10% by in every 100g soil, adding 10ml distilled water.Second part of sterilization soil mud is implanted with the thick slurry of Conidiobolus coronatus spore and mycelia.This slurry is to make by being scraped in the 50ml distilled water by the fungi of the growth of the medium on the Petri dish.Then slurry is added in the soil of sterilization with the ratio that every 100g soil adds 10ml.Each 2 kilograms a collection of soil are divided in 20 plastic cups so that contain 100g soil in each cup again.Ten cups of every batch accept to have used 0.01%1-(2-chloro-5-pyridylmethyl)-2-(nitro imino group) filter paper disk handled of imidazolidine.The filter paper disk that remaining sample acceptance had been soaked in distilled water.The Reticulitermes flavipes that about 1g is lived is added in each sample.Per two, three days observations lethality, activity and conks in the time in a week.The results are shown in the table 5 of these tests.
The result: as might be expected, in control sample, termite is acted normally at 7 days viewing durations.Actual in the processing of only carrying out with fungi is same situation, but observes 20% final lethality.Only handle the lethality reach same degree, but observe the poisoning effect in early days in research with chemical agent.Fungi added the chemical agent processing and reached 100% lethality in 2 day.
Embodiment 6
The interaction of research fungi Conidiobolus coronatus and various known termiticide (listing in the table 6).In these trials, use the 100g soil pattern.According to said method among the embodiment 2 Conidiobolus coronatus is added in half soil pattern.Do not add fungi in second half sample.Then chemical termiticide is added each sample (promptly transplanted the sample of fungi and do not transplanted the sample of fungi), its addition is enough to reach ratio listed in the table 6.Then, 1g subteranean termites Reticulitermes flavipes is added in each sample.Observe these samples with 7 days time.Observed result is listed in the table 6.The carbamates and the organophosphorus compounds that are used for this test almost do not influence termite, and perhaps they are same to the reacting phase that does not have fungi and the soil that is added with fungi.On the contrary, used pyrethroid (cyfluthrin) does not kill termite when not having fungi, but reaches 100% lethality when adding fungi in soil.
Embodiment 7
Only repeat the method for embodiment 6 with the pyrethroid termiticide.The results are shown in the table 7.
The result: used three kinds of pyrethroids (cyfluthrin, fenvalerate and cypermethrin) do not have the soil of fungi all to have sizable active difference for having fungi and transplanting.Cyfluthrin is that the most active compound and all three observed data show that all the termite control in being added with the soil of fungi is higher 10 times than the soil that does not have fungi.
Embodiment 8
With listed result of the test among this embodiment of following grade evaluation and the embodiment 10.
Grade lethality % subjective assessment
1 98-100 is excellent
2 90-97 are fine
3 80-89 are good
4 65-79 are satisfied
5 45-64 are dissatisfied
6 30-44 are dissatisfied
7 20-29 are poor
8 3-19 are poor
9 0-2 are invalid
In this test, use the 100g soil pattern.Some samples add the spore of Conidiobolus coronatus, and some samples add Metarhizium anisopliae(MADA strains, CBS Number 326) spore.The concrete added spore quantity of sample is listed among table 8A and the 8B.Use then at the 1-(2-of variable concentrations chloro-5-pyridylmethyl)-2-(nitro imino group) filter paper disk that soaked in the imidazolidine solution handles these samples.For concrete sample, the concentration that is used for preparing the solution of filter paper disk is listed in table 8A and 8B.Then 1g termite Reticulitermes flavipes is added in each sample.After 7 days, with these samples of above-mentioned grade evaluation.Gained the results are shown among table 8A and the 8B.Their explanations are for Conidiobolus itself (table 8A), every gram soil 10
5Spore density to almost not influence of termite.Yet, only using 1-(2-chloro-5-pyridylmethyl)-2-(nitro imino group) the imidazolidine poison bait is with in the soil that is generally invalid concentration and handles, and the spore concentration in soil is low to moderate 10
1The time reach 100% lethality.This means, when the poison bait that nitroguanidine is handled also adds in the soil pattern, 4 powers of required spore concentration low at least 10.When adding Metarhizium in the soil pattern (table 8B), under situation about the poison bait of nitro processing also being added in the soil pattern, spore concentration can hang down several powers of 10.
Embodiment 9
Step with two different Metaehizium anisoplinae strain-MADA strains (CBS Number 326) and BIO 1020 strains (DSM Number 3384) and Conidiobolus coronatus repetition embodiment 8.Yet, be not to use the nitroguanidine of various concentration, and with filter paper disk at distilled water or 0.001%1-(2-chloro-5-pyridylmethyl)-2-(nitro imino group) the imidazolidine solution soaking.Observe 10 days the results are shown in the table 9.
Embodiment 10
With the Metarhizium anisopliae(MADA strain of consumption shown in the table 10, CBS Number 326) and 1-(2-chloro-5-pyridylmethyl)-2-(nitro imino group) imidazolidine repeats the step of embodiment 8.Observed the results are shown in the table 10 after 5 days adopted and identical grade described in the embodiment 8.
Result among the embodiment 9 and 10 confirms, by adding the 1-(2-chloro-5-pyridylmethyl of low concentration)-2-(nitro imino group) the imidazolidine poison bait, can be reduced to many 10 4 powers with the irrelevant spore concentration of fungi kind or strain, and compare with the soil that the poison bait that only is soaked in water is handled, will reach more effective termite control.
Embodiment 11
With fungi Actinomucor sp., Paecilomyces farinosus and Beauveria bassiana and bacterium Serratia sp repeat the step of embodiment 5.Filter paper disk is immersed in distilled water or 0.001%1-(2-chloro-5-pyridylmethyl)-2-(nitro imino group) in the imidazolidine solution.Observe 7 days the results are shown in the table 11.
In table 11, the listed hierarchical system in hurdle, each frame left side is as follows:
-normal: relatively do not change with untreated control sample.
+ slight: some termites are arranged from the teeth outwards, perhaps feed and some variations are arranged with the road.
++ medium: as many termites to be arranged from the teeth outwards, feed and significantly reduce with the road; Poisoning symptom (torpescence, inharmonious).
+++serious: all termites all from the teeth outwards, not feed and with the road; Poisoning symptom strengthens.
++ ++ cause death: the termite of 95-100% is dead or be about to dead.
Listed numeral is the % lethality in the right hurdle of each frame in table 11.
Embodiment 12
Step with the filter paper disk in several different nitromethane compound that is immersed in various concentration and two kinds of soil repetition embodiment 5.A kind of soil be take from the field bacterium soil arranged.Second kind of soil is autoclaving soil.The concentration of every kind of nitromethane and observed the results are shown in the table 12 after 4 days.
Degree of intoxication is listed in the table 12, uses the hierarchical system identical with table 11.
Table 12
Lethality % after 4 days
Nitromethane nitromethane % initial stage degree sterile soil 1) bacterium soil is arranged
A 0.001 + 10FF 100FF
B 0.1 ++++ 99FF 100FF
0.01 ++++ 25FF 100FF
C 0.1 +++ 62FF 100FF
0.01 +++ 59FF 100FF
0.001 ++ 35FF 100FF
D 0.1 ++++ 96FF 100FF
0.01 +++ 74FF 100FF
E 0.1 ++++ 74FF 100FF
0.01 +++ 42FF 100FF
0.001 +++ 24FF 100FF
F 0.1 +++ 22FF 100FF
0.01 ++ 28FF 100FF
G 0.1 ++++ 41FF 100FF
0.01 +++ 21FF 100FF
H 0.1 ++ 12FF 100FF
0.01 + 6FF 100FF
0.001 - 0 7FF
I 0.1 +++ 18FF 100FF
0.01 +++ 13FF 100FF
0.001 +++ 20FF 100FF
Comparison 0.1% aqueous solvent-0 4
0.1% aqueous solvent water-0 0
1) sterilizing methods of soil obviously is not enough.
" F " expression is Metarhizium and Conidiobolus class owing to having entomophagus fungi, major part, the minimizing naturally of termite number.Each F represents one of twice parallel determination.
Though, described the present invention above in detail, should be appreciated that this details only is in order to illustrate that those skilled in the art can do some variations therein but not break away from spirit of the present invention and exceed scope of the present invention in order to describe.
Claims (33)
1, a kind of going out except that the method for termite is comprising being selected from the chemicals of nitrofuanidine, nitromethane class, pyrazolines and pyrethroid coumpound and (b) predacious fungi and bacterium are applied to the place that there are termite in the place of observing termite or suspection with (a) of effective dose is at least a.
2, the process of claim 1 wherein that chemicals is a kind of nitromethane compounds.
3, the process of claim 1 wherein that chemicals is a 1-(2-chloro-5-pyridylmethyl)-the 2-(Nitromethylene) imidazolidine.
4, the method for claim 3, wherein fungi belongs to fungi for Actinomucor sp..
5, the method for claim 3, wherein fungi is Paecilomyces farinosus.
6, the method for claim 3, wherein fungi is Conidiobolus coronatus.
7, the method for claim 3, wherein fungi is Metarhizium anisopliae.
8, the method for claim 2, wherein fungi is Actinomucor sp..
9, the method for claim 2, wherein fungi is Paecilomyces farinosus.
10, the method for claim 2, wherein fungi is Conidiobolus coronatus.
11, the method for claim 2, wherein fungi is Metarhizium anisopliae.
12, the process of claim 1 wherein that fungi is Actinomucor sp..
13, the process of claim 1 wherein that fungi is Paecilomyces farinosus.
14, the process of claim 1 wherein that fungi is Conidiobolus coronatus.
15, the process of claim 1 wherein that fungi is Metarhizium anisopliae.
16, the composition of control termite is comprising the pest control with insecticide ant agent that is selected from nitrofuanidine, nitromethane class, pyrazolines and pyrethroid coumpound and (2) predacious fungi or the bacterium of (1) effective dose.
17, the composition of claim 16, chemical termiticide wherein are a kind of nitromethane compounds.
18, the composition of claim 17, wherein fungi is Conidiobolus coronatus.
19, the composition of claim 17, wherein fungi is Metarhizium anisopliae.
20, the composition of claim 17, wherein fungi is Actinomycor sp..
21, the composition of claim 17, wherein fungi is Paecilomuces farinosus.
22, be used to go out composition except that termite, it is made up of the following component of effective dose: (a) compound shown at least a formula I:
In the formula, R represents acyl group, the alkyl of hydrogen, acyl group, replacement, alkyl, aryl, aryl, the heterocyclic radical of replacement or the heterocyclic radical that replaces of replacement;
A representative or (1) are selected from the monofunctional group of following groups:
The double functional group that the aryl of the acyl group of hydrogen, acyl group, alkyl, aryl, replacement, the alkyl of replacement, replacement or (2) are connected with Z;
E represents electron withdraw group;
X represents N or CH; And
Z representative or (1) are selected from the monofunctional group of following groups: the heterocyclic radical of the aryl of the alkyl of the acyl group of hydrogen, acyl group, replacement, alkyl, replacement, aryl, replacement, heterocyclic radical, replacement, OR, NR
2, SR, perhaps (2) double functional group of being connected with A or X; And
(b) predacious fungi or bacterium.
23, the composition of claim 22, wherein, in the compound that the formula I is represented,
Z represents NH, CH or NR ' R ", wherein
One of R ' and R " respectively represent hydrogen or alkyl, but R ' and R " at least are alkyl,
A represents alkyl,
E represents CN or NO,
X bit table N or CH,
The R representative is at random by 5 or 6 yuan of heterocycles of fontanel element or alkyl replacement.
24, the composition of claim 22, wherein, in the compound shown in the formula I,
A and Z represent nitrogenous 5 or 6 yuan of sulfur-bearing 5 or the 6 yuan of rings that encircle or replaced by alkylthio arbitrarily that replaced by alkylthio arbitrarily together,
E represents CN or NO
2,
X represents N or CH, and
The R representative is at random by 5 or 6 yuan of heterocycles of fontanel element or alkyl replacement.
25, the composition of claim 22, wherein (b) is fungi.
26, the composition of claim 25, wherein fungi is Paecilomyces farinosus.
27, the composition of claim 25, wherein fungi is Paecilomyces farinosus.
28, the composition of claim 22, wherein (b) is bacterium.
29, be used to go out composition except that termite, it is made up of the following component of effective dose:
(a) compound shown at least a formula II or the III,
In the formula, A representative or (1) are selected from the monofunctional group of following groups: the double functional group that the aryl of the acyl group of hydrogen, acyl group, alkyl, aryl, replacement, the alkyl of replacement, replacement or (2) are connected with Z;
E represents electron withdraw group;
X represents N or CH; And
Z representative or (1) are selected from the monofunctional group of following groups: the heterocyclic radical of the aryl of the alkyl of the acyl group of hydrogen, acyl group, replacement, alkyl, replacement, aryl, replacement, heterocyclic radical, replacement, OR, NR, SR; perhaps (2) double functional group of being connected with A or X
The Y representative has the alkyl of 1-4 carbon atom, the alkoxyl with 1-6 carbon atom, the alkylthio group with 1-4 carbon atom, the fontanel substituted alkyl with 1-4 carbon atom, hydroxyl, fontanel element, amino, alkyl amino, carboxyl, alkoxy carbonyl group, sulfo group, alkyl sulphonyl or aryl sulfonyl, and
N represents 1 or 2,
(b) predacious fungi or bacterium.
30, the composition of claim 29, wherein (b) is predacious fungi.
31, the composition of claim 30, wherein fungi is Actinomucor sp..
32, the composition of claim 30, wherein fungi is Paecilomyces farinosus.
33, the composition of claim 29, wherein (b) is bacterium.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US81832692A | 1992-01-09 | 1992-01-09 | |
| US818,326 | 1992-01-09 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1080474A true CN1080474A (en) | 1994-01-12 |
| CN1053558C CN1053558C (en) | 2000-06-21 |
Family
ID=25225264
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN93100386A Expired - Fee Related CN1053558C (en) | 1992-01-09 | 1993-01-09 | Combined use of Chemicals and microbials in termite control |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JPH05345704A (en) |
| CN (1) | CN1053558C (en) |
| AU (1) | AU652682B2 (en) |
| BR (1) | BR9300040A (en) |
| TW (1) | TW226962B (en) |
| ZA (1) | ZA93127B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1315384C (en) * | 2005-05-16 | 2007-05-16 | 广东省昆虫研究所 | Chemical and method for killing red ant |
| CN1316899C (en) * | 2005-12-26 | 2007-05-23 | 重庆大学 | Mixed insecticide of green muscardine fungus and pyrethrum group and its preparation method |
| CN105794859A (en) * | 2013-01-25 | 2016-07-27 | 陕西汤普森生物科技有限公司 | Insecticidal compound containing conidioblous thromboides and biological sources |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU687383B2 (en) * | 1993-06-02 | 1998-02-26 | Bayer Corporation | Combined use of chemicals and microbials in cockroach control |
| DE10360836A1 (en) * | 2003-12-23 | 2005-07-21 | Bayer Chemicals Ag | Means of protection of technical materials |
| JP2006269808A (en) | 2005-03-24 | 2006-10-05 | Mitsubishi Electric Corp | Semiconductor device and image display device |
| EP2460407A1 (en) * | 2010-12-01 | 2012-06-06 | Bayer CropScience AG | Agent combinations comprising pyridylethyl benzamides and other agents |
| WO2014086756A1 (en) * | 2012-12-03 | 2014-06-12 | Bayer Cropscience Ag | Composition comprising a biological control agent and an insecticide |
| US10219518B2 (en) | 2015-09-18 | 2019-03-05 | Environmental Intellectual Property, Inc. | Inhibition of methanogenesis to control wood boring insects and pestilence |
| US10729132B2 (en) | 2015-09-18 | 2020-08-04 | Environmetal Intellectual Property, Inc. | Xylophage control using antimethanogenic reagents |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2709264C3 (en) * | 1977-03-03 | 1982-01-21 | Bayer Ag, 5090 Leverkusen | Substituted phenoxybenzyloxycarbonyl derivatives, processes for their preparation and their use as insecticides and acaricides and new intermediates |
| US4363798A (en) * | 1981-07-09 | 1982-12-14 | S. C. Johnson & Son, Inc. | Termite bait composition |
| US4751082A (en) * | 1985-04-19 | 1988-06-14 | Bruno Schaerffenberg | Insecticide and method for its distribution |
| DE3639504A1 (en) * | 1986-11-20 | 1988-06-01 | Bayer Ag | PEST CONTROL AND PLANT TREATMENT AGENTS |
| IL95066A (en) * | 1990-07-12 | 1996-01-19 | Peri Dev Applic 1985 Ltd | Fungicidal compositions containing isolate i-952 of trichoderma harzianum t-39 and their use against b cinerea and s sclerotiorum |
-
1993
- 1993-01-04 AU AU31018/93A patent/AU652682B2/en not_active Ceased
- 1993-01-08 BR BR9300040A patent/BR9300040A/en not_active IP Right Cessation
- 1993-01-08 ZA ZA93127A patent/ZA93127B/en unknown
- 1993-01-08 JP JP5016845A patent/JPH05345704A/en not_active Ceased
- 1993-01-09 CN CN93100386A patent/CN1053558C/en not_active Expired - Fee Related
- 1993-01-09 TW TW82100096A patent/TW226962B/zh active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1315384C (en) * | 2005-05-16 | 2007-05-16 | 广东省昆虫研究所 | Chemical and method for killing red ant |
| CN1316899C (en) * | 2005-12-26 | 2007-05-23 | 重庆大学 | Mixed insecticide of green muscardine fungus and pyrethrum group and its preparation method |
| CN105794859A (en) * | 2013-01-25 | 2016-07-27 | 陕西汤普森生物科技有限公司 | Insecticidal compound containing conidioblous thromboides and biological sources |
| CN105794859B (en) * | 2013-01-25 | 2018-11-20 | 陕西汤普森生物科技有限公司 | A kind of Pesticidal combination containing Conidiobolus thromboides and biogenic substances |
Also Published As
| Publication number | Publication date |
|---|---|
| AU3101893A (en) | 1993-07-15 |
| TW226962B (en) | 1994-07-21 |
| CN1053558C (en) | 2000-06-21 |
| ZA93127B (en) | 1993-08-16 |
| JPH05345704A (en) | 1993-12-27 |
| AU652682B2 (en) | 1994-09-01 |
| BR9300040A (en) | 1993-10-05 |
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