CN108029703A - Application of one plant of Bei Laisi bacillus in capsicum epidemic disease is prevented - Google Patents

Application of one plant of Bei Laisi bacillus in capsicum epidemic disease is prevented Download PDF

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CN108029703A
CN108029703A CN201711397714.0A CN201711397714A CN108029703A CN 108029703 A CN108029703 A CN 108029703A CN 201711397714 A CN201711397714 A CN 201711397714A CN 108029703 A CN108029703 A CN 108029703A
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epidemic disease
capsicum epidemic
bacterial strain
plant
bei laisi
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CN108029703B (en
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兰成忠
游泳
杨秀娟
阮宏椿
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Institute of Plant Protection of FAAS
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

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  • General Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
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Abstract

The invention belongs to biological control on crop disease technical field, discloses application of one plant of Bei Laisi bacillus in capsicum epidemic disease is prevented, biocontrol bacterial strain FJ17 4, and Classification And Nomenclature is Bei Laisi bacillus（Bacillusvelezensis）, it is CGMCC N0.14642 in the preserving number of China Committee for Culture Collection of Microorganisms's common micro-organisms center.The bacterium 24 h of shaken cultivation in LB nutrient solutions, the zymotic fluid of gained have capsicum epidemic disease stable prevention effect.The present invention provides new microorganism resource for the biological control of capsicum epidemic disease.

Description

Application of one plant of Bei Laisi bacillus in capsicum epidemic disease is prevented
Technical field
The present invention relates to technical field of plant disease biological control, and in particular to one plant of Bei Laisi bacillus is peppery in prevention Application in green pepper epidemic disease.
Background technology
By phytophthora blight of pepperPhytophthora capsiciIt is a kind of destruction that Leonian, which infects caused capsicum epidemic disease, Property soil-borne disease.In recent years, capsicum epidemic disease endangers getting worse in world many countries, become capsicum production major limitation because Son.Hausbeck etc. is studied with regard to loss of the U.S. different regions capsicum epidemic disease to yield, finds general field yield damage Lose up to 30% or so, serious field almost has no harvest；The researchs such as Silvar show that capsicum epidemic disease is serious in southern Spain area Plot can cause seedling stage plant all dead；The results of study such as Li Zhi army show that production loss the lighter reaches caused by capsicum epidemic disease 20%~30%, severe one is more than more than 80%.The research such as Lan Chengzhong show, capsicum epidemic disease Fujian Province occurrence injury increasingly Seriously, grave illness field loss late reaches 30-100%, and due to factors such as continuous cropping, warm high humiditys, capsicum epidemic disease is brought sternly to pepper planting The loss of weight, general time lose 20-30%, and loss up to 100%, or even cause to have no harvest when serious, which becomes influences It is peppery（Sweet tea）One of major obstacle of green pepper production.
It is prevention capsicum epidemic disease most directly effective method, but phytophthora blight of pepper has abundant physiology using disease-resistant variety Microspecies, most of resistant varieties are difficult the ability for being provided simultaneously with resisting a variety of biological strains, and part highly resistance kind is excellent due to lacking Horticultural Characters its plantation in production is promoted be restricted.In addition, phytophthora blight of pepper belongs to heterothallism oomycetes, exist Asexual and sexual two kinds of modess of reproduction, in the case where host resistance selects pressure there are certain variability, cause cause of disease colony to present Go out the genetic structure of complexity, new biological strain occur, add the selection and breeding difficulty of resistant variety.The prevention of capsicum epidemic disease at present Still based on chemical bactericide, the Reusability of a large amount of chemical pesticides, it is residual to have fitted the serious pollution into environment, agricultural product pesticide Stay and pathogen resistance produces and wildness once again.Therefore, be badly in need of a kind of green control measure in production with ensure agricultural can Sustainable development.Numerous studies show that Bacillus strain there is good biological control to act on plant disease.
The present invention is separated to one plant of bacterium to phytophthora capsici with higher antagonistic ability from soil, through microorganism point Class is accredited as Bei Laisi bacillus（Bacillus velezensis）.The present invention further grinds for microbial pesticide System and exploitation provide excellent starting strain, while to reducing environmental pollution, reducing pesticide residue, avoid the pathogen resistance to the action of a drug from producing Raw and rampant once again and maintenance agricultural sustainable development has great theory and production meaning.
The content of the invention
The seriousness that endangers and chemical bactericide be largely repeatedly in vegetables produce for capsicum epidemic disease for the purpose of the present invention Using there are the drawbacks of, there is provided application of one plant of Bei Laisi bacillus in capsicum epidemic disease is prevented.
The purpose of the present invention can be achieved through the following technical solutions：
One plant of Classification And Nomenclature is Bei Laisi bacillus（Bacillusvelezensis）Biocontrol bacterial strain FJ17-4, in 2017 On September is preserved in positioned at the Chinese micro- of Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica for 18 Biological inoculum preservation administration committee common micro-organisms center, deposit number are CGMCC No.14642.
Present invention also offers a kind of biocontrol agent containing biocontrol bacterial strain FJ17-4, which is shellfish This bacillus FJ17-4 bacterial strain of Lay, bacteria containing amount are 1 × 109~1010CFU/mL。
Present invention also offers a kind of method that biocontrol agent is prepared using biocontrol bacterial strain FJ17-4, its step are as follows：
（1）The biocontrol bacterial strain FJ17-4 of Cord blood is lined on LB culture medium flat plates, when culture 24 is small at 28 DEG C, is obtained The strain of activation；
（2）A ring step is scraped with the oese of sterilizing（1）The inoculation of activation in 100 mL LB fluid nutrient mediums, in 28 DEG C are vibrated in shaking table, and culture is 0.5~0.8 to nutrient solution OD600 under the conditions of 180rmp, obtains seed liquor；
（3）Seed liquor is inoculated in LB fluid nutrient mediums, 28 DEG C, expands 24 h of culture under the conditions of 180rmp, then 4000rpm 10 min are centrifuged, collect thalline, bacterial strain is diluted with aqua sterilisa viable bacteria concentration is made as 1 × 109-1×1010CFU/mL's Bacterium suspension, obtains the biocontrol agent.
Wherein, the inoculum concentration of the seed liquor is preferably 1~2%.The LB solid cultures based formulas is：Tryptone 10 G, 5 g of yeast extract, 5 g of sodium chloride, 15 g of agar powder, water 1000mL, pH 7.2；The LB Liquid Culture based formulas For：10 g of tryptone, 5 g of yeast extract, 5 g of sodium chloride, water 1000mL, pH 7.2.
Biocontrol agent prepared by the biocontrol bacterial strain FJ17-4 that the preserving number is CGMCC No.14642 is in prevention capsicum Application in epidemic disease.The biocontrol agent can be watered spraying or pouring root uses.
Beneficial effects of the present invention：
Compared with prior art, the present invention provides biocontrol bacterial strain and biocontrol agent with preventive and therapeutic effect for capsicum epidemic disease, can Biological control for capsicum epidemic disease.The use of a large amount of chemical pesticides, is conducive to green in being prevented the invention avoids capsicum epidemic disease The production of color vegetables capsicum, this is to reducing the use of chemical bactericide, reducing pollution by pesticides and being avoided pathogen resistance generation etc. Have great importance.
Brief description of the drawings
Fig. 1 is the colonial morphology of biocontrol bacterial strain FJ17-4.
Fig. 2 is the scanning electron microscopic observation figure of biocontrol bacterial strain FJ17-4.
Fig. 3 for bacterial strain FJ17-4 be based on 16SrRNA gene orders comparison result withAlicyclobacillus acidocaldarius DSM 446 (AJ496806) is the Neighbor-Joining phylogenetic trees of outer branch structure；
Fig. 4 is tablet antagonistic effects of the biocontrol bacterial strain FJ17-4 to phytophthora blight of pepper.
Fig. 5 is indoor pot prevention effect of the biocontrol bacterial strain FJ17-4 thallus suspension liquids to capsicum epidemic disease.
Fig. 6 is interior in vitro fruit prevention effect of the biocontrol bacterial strain FJ17-4 thallus suspension liquids to capsicum epidemic disease.
Fig. 7 is field control effect of the biocontrol bacterial strain FJ17-4 thallus suspension liquids to capsicum epidemic disease.
Embodiment
Below in conjunction with specific embodiment, the present invention is further elaborated, but is not limited to the scope of the present invention.Below Embodiment is according to conventional laboratory conditions, or has delivered the operating technology code described in pertinent literature, or is built according to manufacturer The experiment condition of view.
The preparation of the biocontrol agent of 1 bacterial strain containing FJ17-4 of embodiment
1st, the acquisition of biocontrol bacterial strain FJ17-4
Biocontrol bacterial strain FJ17-4 is separated from the local official's Zhuan Cun soil of Fuzhou City, Fujian Province Minqing County Yunlong and obtained, using plating dilutions Method separates the bacterium in gathered soil sample, and the culture medium made is LB（10 g of tryptone, 5 g of yeast extract, chlorine Change sodium 5 g, agar 15g, water 1000mL, pH 7.2）.According to different colonial morphologies, separated bacterium is purified, is isolated Purifying obtains 326 plants of bacteriums.Then using Causal Organism of Cucumber Blight as target pathogenic bacteria, using tablet opposite culture method（Fig. 4）It is pure to institute Change bacterium to be measured the antagonistic ability of Causal Organism of Cucumber Blight, obtain that there is antagonism primary dcreening operation bacterium 18 to Causal Organism of Cucumber Blight Strain.By indoor pot experiment, further the diseases prevention ability of primary dcreening operation bacterial strain is assessed, finally obtain tool antagonism has again 6 plants of the bacterial strain of potted plant preventive and therapeutic effect.Comprehensive antagonism and potted plant diseases prevention ability, it is representative to choose FJ17-4 Antagonistic Fungis, and the bacterium is to peppery Green pepper epidemic disease bacterium mycelial growth has stronger antagonism.
Identify that separated biocontrol bacterial strain FJ17-4 is shellfish Lay through morphology, Physiology and biochemistry and molecular biology 16s rDNA This bacillus（Bacillus velezensis）.Its morphological feature is as shown in Figure 1, 2：Bacterial strain Gram's staining is positive； On LB culture medium flat plates, bacterium colony surface flat, coarse opaque, milky, edge is irregular, and dry tack free has fold, bacterium Fall central concavity projection（Figure）；Its thalline of scanning electron microscopic observation is in rod-short（Figure）, optimum growth temperature is 28 DEG C；Its physiology is given birth to Change feature as shown in table 1- tables 3, its 16s rDNA sequence is compared based on 16SrRNA gene orders and tied as shown in SEQ ID No.1 Fruit phylogenetic tree is as shown in Figure 2.
1 bacterial strain FJ17-4 physio-biochemical characteristics of table-enzyme activity, carbon source oxidation
+：Positive reaction； -：Negative reaction；W：Weakly positive is reacted
2 bacterial strain FJ17-4 physio-biochemical characteristics of table-utilize carbon source production acid
+：The positive ,-：Feminine gender, w：Weakly positive
3 bacterial strain FJ17-4 physio-biochemical characteristics of table-utilization of carbon source
+：The positive ,-：Feminine gender, w：Weakly positive
2nd, the preparation of biocontrol agent
1）The biocontrol bacterial strain FJ17-4 of Cord blood is lined on LB culture medium flat plates, when culture 24 is small at 28 DEG C, is obtained The strain of activation.The constituent and its weight ratio of LB solid mediums be：10 g of tryptone, 5 g of yeast extract, sodium chloride 5 g, agar powder 16g, water 1000mL, pH 7.2；
2）A ring step is scraped with the oese of sterilizing（1）The inoculation of activation is in 100 mL LB fluid nutrient mediums, Yu Zhen 28 DEG C are swung in shaking table, and culture is 0.7 to nutrient solution OD600 under the conditions of 180rmp, obtains seed liquor.LB Liquid Culture based formulas For：10 g of tryptone, 5 g of yeast extract, 5 g of sodium chloride, water 1000mL, pH 7.2；
3）By seed liquor with 1:1000 volume ratios are inoculated in LB fluid nutrient mediums, 28 DEG C, expand culture 24 under the conditions of 180rmp H, then 4000rpm centrifuge 10 min, collect thalline, bacterial strain is diluted with aqua sterilisa be made viable bacteria concentration for 1 × 1010The bacterium suspension of CFU/mL, obtains the biocontrol agent.
Embodiment 2：Antagonistic activity of the FJ17-4 bacterial strains to phytophthora capsici
With opposite culture method, the phytophthora blight of pepper being stored in 4 DEG C is inoculated on PDA plate and is activated, treat that fungi covers with tablet Uniformly break into the circular fungus block of a diameter of 5 mm from bacterium colony outer edge with the card punch of sterilizing afterwards.Inoculated by hypha block is being improved NB culture medium flat plates（Peptone 5g, glucose 10g, gravy medicinal extract 3g, sodium chloride 5g, agar 20g, distilled water 1000mL, pH =7.2）Center, is about inoculated with FJ17-4 away from center in its surrounding, tests using clear water as control group respectively at 25mm.25 DEG C of cultures, are treated Mycelia is observed after covering with tablet and records the size of inhibition zone.
The activity of FJ17-4 antagonism phytophthora capsicis is assessed according to the size of inhibition zone：0 mm, unrestraint effect； ＜ 2mm, there is slight inhibitory action；2-5mm, medium inhibitory action；＞ 5mm, stronger inhibitory action.According to pathogen short of money Growth diameter on antibacterial direction, calculates inhibiting rates of the FJ17-4 to phytophthora capsici, and inhibiting rate algorithm is as follows：Inhibiting rate= [(50-R)/50] × 100%, wherein, because capsicum epidemic disease mycelia block is inoculated in tablet center, and Antagonistic Fungi vaccination is from mycelia The distance of block is 25mm, under conditions of Antagonistic Fungi does not have any antagonism, the bacterium of growth of pathogenic bacteria to Antagonistic Fungi vaccination Fall diameter and should be 50mm, R is colony radius of the pathogen in bacterium direction.
Testing result is shown in Fig. 4.From fig. 4, it can be seen that biocontrol microorganisms FJ17-4 there is stronger suppression to imitate phytophthora capsici growth Fruit, it is 15mm to suppress circle radius, percentage mycelial inhibition 60%.Compared with negative control clear water treatment group, antagonistic effect ten is clearly demarcated It is aobvious.
Embodiment 3：FJ17-4 bacterial strains（Biocontrol agent）To the prevention effect of cucumber fusarium axysporum
1）Indoor pot experiment prevention effect
13 Sweet Pepper Varieties of lotus green pepper are seeded in 50 lattice seedlings nursing plates, 1 plant will be stayed per cave after emergence, treat that pimento seedling grows to 4 very Ye Shi, is 1 × 10 by sporangium concentration7For the capsicum epidemic disease bacterium suspension pouring root of/mL in Hot Pepper Seedling root, every 10mL will FJ17-4 bacterial strain fermentation liquors（Cell concentration is 1 × 1010CFU/mL）Pour in the seedling root for being inoculated with phytophthora capsici, every plant 10mL, using 58% metalaxyl mancozeb wettable powder, 700 times of every plant of 10mL as control treatment；Not make any processing The Hot Pepper Seedling for being inoculated with phytophthora capsici is blank control.After 10 days, the incidence of each treatment group is counted, calculates prevention effect. Incidence（%）=（Morbidity strain number/always investigate strain number）× 100%, prevention effect（%）=[（Blank control incidence-processing morbidity Rate）/ blank control incidence] × 100%.
By Fig. 5 and table 4 as it can be seen that FJ17-4 bacterial strain bacteria suspensions（Biocontrol agent）With 58% metalaxyl Mancozeb wettable Pulvis 700 has certain prevention effect to the capsicum epidemic disease of indoor pot experiment again, and prevention effect is respectively 81.63% He The prevention effect of 77.55%, FJ17-4 bacterial strain bacteria suspension is slightly above the anti-of 700 times of 58% metalaxyl mancozeb wettable powder Control effect.
Indoor pot prevention effect of the 4 Bei Laisi bacillus FJ17-4 bacteria suspensions of table to capsicum epidemic disease
2）Indoor in vitro fruit prevention effect
The 13 pimento fruit of lotus green pepper that can be listed is gathered, surface sterilization is carried out to the pepper fruit gathered using 70% ethanol.With going out Bacterium knife blade lightly causes miniature wound to be used for wound inoculation method and is inoculated with phytophthora capsici, each wound point in fruit surface The phytophthora capsici mycelia block 1 of a diameter of 5mm is inoculated with, moisturizing culture under the conditions of being 28 DEG C of 100% in relative humidity.24 is small Shi Hou, removes the mycelia block on pepper fruit surface, by FJ17-4 bacterial strain fermentation liquors（Cell concentration is 1 × 1010CFU/mL）Spray In fruit surface, the amount of spraying is subject to fruit surface with water droplets, and 58% metalaxyl Mancozeb wettable is sprayed with fruit surface 700 times of control treatments of pulvis；Blank control is used as using fruit surface spray clear water.All processing fruits are placed in relative humidity Moisturizing culture under the conditions of 28 DEG C of 100%.After 5 days, the lesion diameter of each treatment group fruit surface is counted, calculates prevention effect.It is anti- Control effect（%）=[（Blank control lesion diameter-processing lesion diameter）/ blank control lesion diameter] × 100%.
By Fig. 6 and table 5 as it can be seen that FJ17-4 bacterial strain bacteria suspensions（Biocontrol agent）With 58% metalaxyl Mancozeb wettable Pulvis 700 has certain prevention effect to the capsicum epidemic disease of in vitro fruit laboratory test again, and prevention effect is respectively 83.33% He The prevention effect of 72.22%, FJ17-4 bacterial strain bacteria suspension is higher than the prevention of 700 times of 58% metalaxyl mancozeb wettable powder Effect.
Interior in vitro fruit prevention effect of the 5 Bei Laisi bacillus FJ17-4 bacteria suspensions of table to capsicum epidemic disease
3）Control in field effect
Daejeon prevention test selection former years capsicum epidemic disease occur more serious Fujian Province Jianou City Dong Feng townshiies into Kun Kou villages into OK.Spraying treatment is carried out in capsicum epidemic disease early stage, experimental design is essentially identical with laboratory test, is set to 3 groups, and the 1st group makes Carry out spraying treatment with FJ17-4 bacterial strains bacteria suspension, the 2nd group using 58% metalaxyl mancozeb wettable powder 700 again into Row spraying control treatment, the 3rd group is to blank control using clear water spraying, and formulation rate was subject to blade and drips, every medication in 7 days 1 time, administration time is respectively on April 22nd, 2017, April 29 and May 6, totally 3 times, after last 1 medication terminates 10 days （On May 16th, 2017）The disease index of field capsicum epidemic disease is investigated, calculates the prevention effect of each processing.Specific method is such as Under：All plant in every cell are investigated.Record investigation strain number, morbidity strain number and sick series, calculate disease index.Point Level standard is as follows：0 grade：Health is without disease；1 grade：Overground part only leaf, fruit have scab；3 grades：The aerial stem of plant, branch have brown rot spot；5 grades：Stem Base portion has brown rot spot；7 grades：The aerial stem of plant, branch and basal part of stem have brown rot spot, and part branch is withered；9 grades：Complete stool is withered.Root The sick series of total strain number, morbidity strain number and leaf of falling ill according to investigations；Calculate disease index；Treatment region and check plot disease index compare Calculate preventive effect.Disease index=∑（Diseased plant numbers at different levels × relative disease value of series）÷（Investigate total strain number × 9）×100；Prevention effect （%）=（CK disease indexs-processing disease index）÷ CK disease indexs ］ × 100
From Fig. 7, table 6 as it can be seen that biocontrol bacterial strain FJ17-4 bacteria suspensions（Biocontrol agent）With 58% metalaxyl Mancozeb wettable powder 700 times of agent is right（Crop field）Capsicum epidemic disease there is certain prevention effect, prevention effect is respectively 80.31% and 74.5%, FJ17- 4 bacterial strain bacteria suspensions（Biocontrol agent）Prevention effect higher than 700 times of 58% metalaxyl mancozeb wettable powder prevention imitate Fruit, shows preferable application prospect.
Field control effect of the 6 Bei Laisi bacillus FJ17-4 bacteria suspensions of table to capsicum epidemic disease
SEQUENCE LISTING
<110>Inst. of Plant Protection, fujian Academy of Agricultural Science
<120>Application of one plant of Bei Laisi bacillus in capsicum epidemic disease is prevented
<130> 1
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1447
<212> DNA
<213>16s rDNA sequences
<400> 1
gggcggcgtg cctatacatg cagtcgagcg gacagatggg agcttgctcc ctgatgttag 60
cggcggacgg gtgagtaaca cgtgggtaac ctgcctgtaa gactgggata actccgggaa 120
accggggcta ataccggatg gttgtttgaa ccgcatggtt cagacataaa aggtggcttc 180
ggctaccact tacagatgga cccgcggcgc attagctagt tggtgaggta acggctcacc 240
aaggcgacga tgcgtagccg acctgagagg gtgatcggcc acactgggac tgagacacgg 300
cccagactcc tacgggaggc agcagtaggg aatcttccgc aatggacgaa agtctgacgg 360
agcaacgccg cgtgagtgat gaaggttttc ggatcgtaaa gctctgttgt tagggaagaa 420
caagtgccgt tcaaataggg cggcaccttg acggtaccta accagaaagc cacggctaac 480
tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggaat tattgggcgt 540
aaagggctcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600
gtcattggaa actggggaac ttgagtgcag aagaggagag tggaattcca cgtgtagcgg 660
tgaaatgcgt agagatgtgg aggaacacca gtggcgaagg cgactctctg gtctgtaact 720
gacgctgagg agcgaaagcg tggggagcga acaggattag ataccctggt agtccacgcc 780
gtaaacgatg agtgctaagt gttagggggt ttccgcccct tagtgctgca gctaacgcat 840
taagcactcc gcctggggag tacggtcgca agactgaaac tcaaaggaat tgacgggggc 900
ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960
ttgacatcct ctgacaatcc tagagatagg acgtcccctt cgggggcaga gtgacaggtg 1020
gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca 1080
acccttgatc ttagttgcca gcattcagtt gggcactcta aggtgactgc cggtgacaaa 1140
ccggaggaag gtggggatga cgtcaaatca tcatgcccct tatgacctgg gctacacacg 1200
tgctacaatg gacagaacaa agggcagcga aaccgcgagg ttaagccaat cccacaaatc 1260
tgttctcagt tcggatcgca gtctgcaact cgactgcgtg aagctggaat cgctagtaat 1320
cgcggatcag catgccgcgg tgaatacgtt cccgggcctt gtacacaccg cccgtcacac 1380
cacgagagtt tgtaacaccc gaagtcggtg aggtaacctt taggagccag ccgccgaagt 1440
ggaccta 1447

Claims (3)

1. application of one plant of Bei Laisi bacillus in capsicum epidemic disease is prevented.
2. application according to claim 1, it is characterised in that：The bacterium is Bei Laisi bacillus （Bacillusvelezensis）FJ17-4, China Committee for Culture Collection of Microorganisms is preserved on 18th in September in 2017 Common micro-organisms center, deposit number are CGMCC No.14642.
3. application according to claim 2, it is characterised in that：By the Bei Laisi bacillus （Bacillusvelezensis）FJ17-4 is prepared into biocontrol agent, preparation method be by FJ17-4 28 DEG C in LB nutrient solutions, 24 h of 180rpm shaken cultivations, then 4000 rpm centrifugations, 10 min, collect the bacterial strain in precipitation, are carried out bacterial strain with aqua sterilisa Viable bacteria concentration is made as 1 × 10 in dilution9-1×1010The bacterium suspension of CFU/mL.
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108753664A (en) * 2018-06-28 2018-11-06 广东省农业科学院农业资源与环境研究所 A kind of biocontrol agent, biological organic fertilizer and its application
CN109055281A (en) * 2018-09-19 2018-12-21 北京化工大学 Bei Laisi bacillus ZF2 and its application in control of plant disease
CN110184221A (en) * 2019-06-05 2019-08-30 中国农业科学院蔬菜花卉研究所 One plant of Bei Laisi bacillus and its application in prevention and treatment tomato phytophthora root rot
CN110564646A (en) * 2019-09-20 2019-12-13 湖北省生物农药工程研究中心 Bacillus belgii CY30 and application thereof in prevention and treatment of tea round spot
CN111073825A (en) * 2018-10-19 2020-04-28 沈阳药科大学 Bacterium with plant soil-borne disease resistance effect and application thereof
CN115029269A (en) * 2022-06-21 2022-09-09 新疆农业大学 Pear fire blight bacterium antagonistic bacterium for producing lipopeptide antibiotics and fermentation method and application thereof

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Publication number Priority date Publication date Assignee Title
CN108753664A (en) * 2018-06-28 2018-11-06 广东省农业科学院农业资源与环境研究所 A kind of biocontrol agent, biological organic fertilizer and its application
CN109055281A (en) * 2018-09-19 2018-12-21 北京化工大学 Bei Laisi bacillus ZF2 and its application in control of plant disease
CN111073825A (en) * 2018-10-19 2020-04-28 沈阳药科大学 Bacterium with plant soil-borne disease resistance effect and application thereof
CN110184221A (en) * 2019-06-05 2019-08-30 中国农业科学院蔬菜花卉研究所 One plant of Bei Laisi bacillus and its application in prevention and treatment tomato phytophthora root rot
CN110564646A (en) * 2019-09-20 2019-12-13 湖北省生物农药工程研究中心 Bacillus belgii CY30 and application thereof in prevention and treatment of tea round spot
CN110564646B (en) * 2019-09-20 2020-05-05 湖北省生物农药工程研究中心 Bacillus belgii CY30 and application thereof in prevention and treatment of tea round spot
CN115029269A (en) * 2022-06-21 2022-09-09 新疆农业大学 Pear fire blight bacterium antagonistic bacterium for producing lipopeptide antibiotics and fermentation method and application thereof
CN115029269B (en) * 2022-06-21 2024-01-30 新疆农业大学 Lipopeptid antibiotic-producing erwinia amylovora antagonistic bacterium, and fermentation method and application thereof

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