CN108029703B - Application of one plant of Bei Laisi bacillus in prevention and treatment capsicum epidemic disease - Google Patents
Application of one plant of Bei Laisi bacillus in prevention and treatment capsicum epidemic disease Download PDFInfo
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- CN108029703B CN108029703B CN201711397714.0A CN201711397714A CN108029703B CN 108029703 B CN108029703 B CN 108029703B CN 201711397714 A CN201711397714 A CN 201711397714A CN 108029703 B CN108029703 B CN 108029703B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
Abstract
The invention belongs to biological control on crop disease technical field, disclose application of one plant of Bei Laisi bacillus in prevention and treatment capsicum epidemic disease, biocontrol bacterial strain FJ17-4, classification naming be Bei Laisi bacillus (Bacillusvelezensis), it is CGMCC N0.14642 in the deposit number of China Committee for Culture Collection of Microorganisms's common micro-organisms center.The bacterium 24 h of shaken cultivation in LB culture solution, resulting fermentation liquid have stable control efficiency to capsicum epidemic disease.The present invention provides new microorganism resource for the biological control of capsicum epidemic disease.
Description
Technical field
The present invention relates to technical field of plant disease biological control, and in particular to one plant of Bei Laisi bacillus is peppery in prevention and treatment
Application in green pepper epidemic disease.
Background technique
By phytophthora blight of pepperPhytophthora capsiciCapsicum epidemic disease caused by Leonian infects is a kind of destruction
Property soil-borne disease.In recent years, capsicum epidemic disease world many countries harm get worse, become capsicum production major limitation because
Son.Hausbeck etc. studies the loss of yield with regard to U.S. different regions capsicum epidemic disease, finds general field yield damage
It loses up to 30% or so, serious field almost has no harvest；Silvar etc. is studies have shown that serious in southern Spain area capsicum epidemic disease
Plot can cause seedling stage plant all dead；The results of study such as Li Zhi army show that production loss less serious case caused by capsicum epidemic disease reaches
20%~30%, severe one is more than 80% or more.The research such as Lan Chengzhong show, capsicum epidemic disease Fujian Province occurrence injury increasingly
Seriously, grave illness field loss late reaches 30-100%, and due to factors such as continuous cropping, warm high humiditys, capsicum epidemic disease is brought sternly to pepper planting
The loss of weight, general time lose 20-30%, loss up to 100% when serious, or even cause to have no harvest, which has become influence
One of the major obstacle of peppery (sweet tea) green pepper production.
It is the most direct effective method of prevention and treatment capsicum epidemic disease using disease-resistant variety, but there are physiology abundant for phytophthora blight of pepper
Microspecies, most of resistant varieties are difficult to be provided simultaneously with the ability for resisting a variety of biological strains, and part highly resistance kind is excellent due to lacking
Horticultural Characters make its in production plantation popularization be restricted.In addition, phytophthora blight of pepper belongs to heterothallism oomycetes, exist
Asexual and sexual two kinds of modess of reproduction, in the case where host resistance selects pressure, there are certain variability, and cause of disease group is caused to present
, there is new biological strain, increases the breeding difficulty of resistant variety in complicated genetic structure out.The prevention and treatment of capsicum epidemic disease at present
Still based on chemical bactericide, the Reusability of a large amount of chemical pesticides has been fitted residual at the serious pollution of environment, agricultural product pesticide
It stays and pathogen resistance generates and wildness once again.Therefore, be badly in need of a kind of control measure of green in production with ensure agricultural can
Sustainable development.A large number of studies show that Bacillus strain has good biological control effect to plant disease.
The present invention is separated to one plant of bacterium to phytophthora capsici with higher antagonistic ability from soil, through microorganism point
Class be accredited as Bei Laisi bacillus (Bacillus velezensis).The present invention is further grinding for microbial pesticide
System and exploitation provide excellent starting strain, while to reducing environmental pollution, reduce pesticide residue, pathogen drug resistance is avoided to produce
Raw and rampant once again and maintenance agricultural sustainable development has great theory and production meaning.
Summary of the invention
The purpose of the present invention is directed to the seriousness that capsicum epidemic disease endangers in vegetables production and chemical bactericide largely repeatedly
Using there are the drawbacks of, provide one plant of Bei Laisi bacillus prevention and treatment capsicum epidemic disease in application.
The purpose of the present invention can be achieved through the following technical solutions:
One plant of classification naming be Bei Laisi bacillus (Bacillusvelezensis) biocontrol bacterial strain FJ17-4, in
It is preserved in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica on September 18th, 2017
State's Microbiological Culture Collection administration committee common micro-organisms center, deposit number are CGMCC No.14642.
The present invention also provides a kind of biocontrol agent containing biocontrol bacterial strain FJ17-4, which is shellfish
This bacillus FJ17-4 bacterial strain of Lay, bacteria containing amount are 1 × 109~1010CFU/mL。
The present invention also provides a kind of methods for preparing biocontrol agent using biocontrol bacterial strain FJ17-4, and its step are as follows:
(1) the biocontrol bacterial strain FJ17-4 of cryo-conservation is lined on LB culture medium flat plate, is cultivated 24 hours at 28 DEG C,
The strain activated；
(2) strain inoculated of a ring step (1) activation is scraped in 100 mL LB liquid mediums with the oese of sterilizing
In, 28 DEG C in oscillation shaking table, culture is 0.5~0.8 to culture solution OD600 under the conditions of 180rmp, obtains seed liquor；
(3) seed liquor is inoculated in LB liquid medium, 28 DEG C, expands 24 h of culture under the conditions of 180rmp, then
4000rpm is centrifuged 10 min, collects thallus, and being diluted bacterial strain with aqua sterilisa and viable bacteria concentration is made is 1 × 109-1×
1010The bacterium suspension of CFU/mL obtains the biocontrol agent.
Wherein, the inoculum concentration of the seed liquor is preferably 1~2%.The LB solid culture based formulas are as follows: tryptone 10
G, 5 g of yeast extract, 5 g of sodium chloride, 15 g of agar powder, water 1000mL, pH 7.2；The LB Liquid Culture based formulas
Are as follows: 10 g of tryptone, 5 g of yeast extract, 5 g of sodium chloride, water 1000mL, pH 7.2.
Biocontrol agent prepared by the biocontrol bacterial strain FJ17-4 that the deposit number is CGMCC No.14642 is in prevention and treatment capsicum
Application in epidemic disease.The biocontrol agent can be watered spraying or pouring root and use.
Beneficial effects of the present invention:
Compared with prior art, the present invention provides biocontrol bacterial strain and biocontrol microorganisms with preventive and therapeutic effect for capsicum epidemic disease
Agent can be used for the biological control of capsicum epidemic disease.The invention avoids the uses of a large amount of chemical pesticides in capsicum epidemic disease prevention and treatment, favorably
In the production of green vegetables capsicum, this is to reducing the use of chemical bactericide, reduce pollution by pesticides and pathogen resistance is avoided to produce
It is raw etc. to have great importance.
Detailed description of the invention
Fig. 1 is the colonial morphology of biocontrol bacterial strain FJ17-4.
Fig. 2 is the scanning electron microscopic observation figure of biocontrol bacterial strain FJ17-4.
Fig. 3 be bacterial strain FJ17-4 be based on 16SrRNA gene order comparison result withAlicyclobacillus acidocaldarius DSM 446 (AJ496806) is the Neighbor-Joining phylogenetic tree of outer branch building；
Fig. 4 is plate antagonistic effect of the biocontrol bacterial strain FJ17-4 to phytophthora blight of pepper.
Fig. 5 is indoor pot control efficiency of the biocontrol bacterial strain FJ17-4 thallus suspension liquid to capsicum epidemic disease.
Fig. 6 is biocontrol bacterial strain FJ17-4 thallus suspension liquid to the in vitro fruit control efficiency in the interior of capsicum epidemic disease.
Fig. 7 is field control effect of the biocontrol bacterial strain FJ17-4 thallus suspension liquid to capsicum epidemic disease.
Specific embodiment
Below in conjunction with specific embodiment, the present invention is further elaborated, but is not intended to limit the scope of the invention.Below
Embodiment is according to conventional laboratory conditions, or has delivered operating technology regulation described in pertinent literature, or built according to manufacturer
The experiment condition of view.
The preparation of the biocontrol agent of 1 bacterial strain containing FJ17-4 of embodiment
1, the acquisition of biocontrol bacterial strain FJ17-4
Biocontrol bacterial strain FJ17-4 is separated from the local official's Zhuan Cun soil of Fuzhou City, Fujian Province Minqing County Yunlong and is obtained, using plate
Dilution method separates the bacterium in acquired soil sample, 10 g of culture medium LB(tryptone made, yeast extract 5
G, sodium chloride 5 g, agar 15g, water 1000mL, pH 7.2).According to different colonial morphologies, separated bacterium is purified, altogether
It isolates and purifies to obtain 326 plants of bacteriums.Then right using plate opposite culture method (Fig. 4) using Causal Organism of Cucumber Blight as target pathogenic bacteria
Purified bacterium is measured the antagonistic ability of Causal Organism of Cucumber Blight, obtains having antagonism primary dcreening operation bacterium to Causal Organism of Cucumber Blight
18 plants.By indoor pot experiment, further the disease prevention ability of primary dcreening operation bacterial strain is assessed, finally obtains tool antagonism again
There are 6 plants of bacterial strain of potting preventive and therapeutic effect.Comprehensive antagonism and potting disease prevention ability, selection FJ17-4 Antagonistic Fungi are representative, the bacterium pair
The growth of phytophthora capsici mycelia has stronger antagonism.
It is identified through morphology, Physiology and biochemistry and molecular biology 16s rDNA, separated biocontrol bacterial strain FJ17-4 is Bei Lai
This bacillus (Bacillus velezensis).Its morphological feature is as shown in Figure 1, 2: bacterial strain Gram's staining is positive；
On LB culture medium flat plate, bacterium colony surface flat, coarse opaque, milky, edge is irregular, and dry tack free has fold, bacterium
Fall central concavity protrusion (figure)；Its thallus of scanning electron microscopic observation is in rod-short (figure), and optimum growth temperature is 28 DEG C；Its physiology is raw
Change feature as shown in table 1- table 3,16s rDNA sequence compares knot as shown in SEQ ID No.1, based on 16SrRNA gene order
Fruit phylogenetic tree is as shown in Figure 2.
1 bacterial strain FJ17-4 physio-biochemical characteristics of table-enzyme activity, carbon source oxidation
+: positive reaction；: negative reaction；W: weakly positive reaction
2 bacterial strain FJ17-4 physio-biochemical characteristics of table-produce acid using carbon source
+: it is positive ,-: negative, w: weakly positive
3 bacterial strain FJ17-4 physio-biochemical characteristics of table-utilization of carbon source
+: it is positive ,-: negative, w: weakly positive
2, the preparation of biocontrol agent
1) the biocontrol bacterial strain FJ17-4 of cryo-conservation is lined on LB culture medium flat plate, is cultivated 24 hours at 28 DEG C,
The strain activated.The constituent and its weight ratio of LB solid medium are as follows: 10 g of tryptone, 5 g of yeast extract, chlorine
Change 5 g of sodium, agar powder 16g, water 1000mL, pH 7.2；
2) with the strain inoculated of oese scraping one ring step (1) activation of sterilizing in 100 mL LB liquid mediums,
28 DEG C in oscillation shaking table, culture is 0.7 to culture solution OD600 under the conditions of 180rmp, obtains seed liquor.LB fluid nutrient medium
Formula are as follows: 10 g of tryptone, 5 g of yeast extract, 5 g of sodium chloride, water 1000mL, pH 7.2；
3) seed liquor is inoculated in LB liquid medium, 28 DEG C with 1:1000 volume ratio, expands training under the conditions of 180rmp
Support 24 h, then 4000rpm is centrifuged 10 min, collects thallus, bacterial strain is diluted with aqua sterilisa be made viable bacteria concentration be 1 ×
1010The bacterium suspension of CFU/mL obtains the biocontrol agent.
Antagonistic activity of the embodiment 2:FJ17-4 bacterial strain to phytophthora capsici
With opposite culture method, the phytophthora blight of pepper being stored in 4 DEG C is inoculated on PDA plate and is activated, is covered with to fungi
The round fungus block that diameter is 5 mm is uniformly broken into from bacterium colony outer edge with the punch of sterilizing after plate.Inoculated by hypha block is existed
NB culture medium flat plate (peptone 5g, glucose 10g, gravy medicinal extract 3g, sodium chloride 5g, agar 20g, the distilled water of improvement
1000mL, pH=7.2) center, in its surrounding away from FJ17-4 is inoculated at center about 25mm respectively, test is using clear water as control group.25
DEG C culture, the size of inhibition zone is observed and recorded after mycelia covers with plate.
It is assessed according to activity of the size of inhibition zone to FJ17-4 antagonism phytophthora capsici: 0 mm, unrestraint effect；
< 2mm has slight inhibiting effect；2-5mm, medium inhibiting effect；> 5mm, stronger inhibiting effect.According to pathogen short of money
Growth diameter on antibacterial direction calculates FJ17-4 to the inhibiting rate of phytophthora capsici, and inhibiting rate algorithm is as follows: inhibiting rate=
[(50-R)/50] × 100%, wherein because capsicum epidemic disease mycelia block is inoculated in plate center, and Antagonistic Fungi vaccination is from mycelia
The distance of block is 25mm, under conditions of Antagonistic Fungi does not have any antagonism, the bacterium of growth of pathogenic bacteria to Antagonistic Fungi vaccination
Falling diameter should be 50mm, and R is colony radius of the pathogen in bacterium direction.
Testing result is shown in Fig. 4.From fig. 4, it can be seen that biocontrol microorganisms FJ17-4 has stronger inhibition effect to phytophthora capsici growth
Fruit, inhibiting circle radius is 15mm, percentage mycelial inhibition 60%.Compared with negative control clear water processing group, antagonistic effect ten is clearly demarcated
It is aobvious.
Control efficiency of the embodiment 3:FJ17-4 bacterial strain (biocontrol agent) to cucumber fusarium axysporum
1) indoor pot experiment control efficiency
13 Sweet Pepper Varieties of lotus green pepper are seeded in 50 lattice seedlings nursing plates, cave every after emergence is stayed 1 plant, grows to 4 to pimento seedling
It is 1 × 10 by sporangium concentration when piece true leaf7The capsicum epidemic disease bacterium suspension pouring root of/mL is in Hot Pepper Seedling root, every 10mL,
By FJ17-4 bacterial strain fermentation liquor, (cell concentration is 1 × 1010CFU/mL it) pours in the seedling root for being inoculated with phytophthora capsici, every plant
10mL, using 58% metalaxyl mancozeb wettable powder, 700 times of every plant of 10mL as control treatment；With with no treatment
The Hot Pepper Seedling for being inoculated with phytophthora capsici is blank control.After 10 days, the disease incidence of each processing group is counted, calculates control efficiency.
Disease incidence (%)=(morbidity strain number/always investigate strain number) × 100%, control efficiency (%)=[(blank control disease incidence-processing morbidity
Rate)/blank control disease incidence] × 100%.
By Fig. 5 and table 4 as it can be seen that FJ17-4 bacterial strain bacteria suspension (biocontrol agent) and 58% metalaxyl Mancozeb wettable
Pulvis 700 has certain control efficiency to the capsicum epidemic disease of indoor pot experiment again, and control efficiency is respectively 81.63% He
The control efficiency of 77.55%, FJ17-4 bacterial strain bacteria suspension is slightly above the anti-of 700 times of 58% metalaxyl mancozeb wettable powder
Control effect.
Indoor pot control efficiency of the 4 Bei Laisi bacillus FJ17-4 bacteria suspension of table to capsicum epidemic disease
2) indoor in vitro fruit control efficiency
The 13 pimento fruit of lotus green pepper that can be listed is acquired, surface sterilization is carried out to pepper fruit collected using 70% ethyl alcohol.
Lightly miniature wound is caused to be inoculated with phytophthora capsici, Mei Gechuan for wound inoculation method in fruit surface with sterilizing knife blade
The phytophthora capsici mycelia block 1 that wound point inoculation diameter is 5mm, moisturizing culture under the conditions of relative humidity is 28 DEG C of 100%.
After 24 hours, the mycelia block on pepper fruit surface is removed, (cell concentration is 1 × 10 by FJ17-4 bacterial strain fermentation liquor10CFU/mL)
It is sprayed on fruit surface, the amount of spraying is subject to fruit surface with water droplets, and spraying 58% metalaxyl Mancozeb with fruit surface can
700 times of control treatments of wet powder；Clear water is sprayed as blank control using fruit surface.All processing fruits are placed in relatively wet
Moisturizing culture under the conditions of 28 DEG C of degree 100%.After 5 days, the lesion diameter of each processing group fruit surface is counted, calculates control efficiency.
Control efficiency (%)=[(blank control lesion diameter-processing lesion diameter)/blank control lesion diameter] × 100%.
By Fig. 6 and table 5 as it can be seen that FJ17-4 bacterial strain bacteria suspension (biocontrol agent) and 58% metalaxyl Mancozeb wettable
Pulvis 700 has certain control efficiency to the capsicum epidemic disease of in vitro fruit laboratory test again, and control efficiency is respectively 83.33% He
The control efficiency of 72.22%, FJ17-4 bacterial strain bacteria suspension is higher than the prevention and treatment of 700 times of 58% metalaxyl mancozeb wettable powder
Effect.
5 Bei Laisi bacillus FJ17-4 bacteria suspension of table is to the in vitro fruit control efficiency in the interior of capsicum epidemic disease
3) control in field effect
More serious Fujian Province, the township Dong Feng, Jianou City occurs for daejeon prevention test selection into female mouth in former years capsicum epidemic disease
Village carries out.Spraying treatment is carried out in capsicum epidemic disease early stage, experimental design is essentially identical with laboratory test, it is set as 3 groups, the 1st
Group carries out spraying treatment using FJ17-4 bacterial strain bacteria suspension, and the 2nd group uses 58% metalaxyl mancozeb wettable powder 700
Carry out spraying control treatment again, the 3rd group is to blank control using clear water by spraying, and formulation rate was subject to blade and drips, every 7 days
Medication 1 time, administration time is respectively on April 22nd, 2017, April 29 and May 6, and totally 3 times, last 1 medication terminates 10 days
The disease index of (on May 16th, 2017) investigation field capsicum epidemic disease afterwards, calculates the control efficiency of each processing.Specific method is such as
Under: all plant in every cell are investigated.Record investigation strain number, morbidity strain number and sick series, calculate disease index.Point
Grade standard is as follows: 0 grade: health is without disease；1 grade: only leaf, fruit have scab to overground part；3 grades: the aerial stem of plant, branch have brown rot spot；5 grades: stem
Base portion has brown rot spot；7 grades: the aerial stem of plant, branch and basal part of stem have brown rot spot, and part branch is withered；9 grades: complete stool is withered.Root
The sick series of total strain number, morbidity strain number and leaf of falling ill according to investigations；Calculate disease index；Treatment region and check plot disease index compare
Calculate preventive effect.Disease index=∑ (diseased plant numbers at different levels × relative disease value of series) ÷ (investigation total strain number × 9) × 100；Control efficiency
(%)=[ (CK disease index-processing disease index) ÷ CK disease index ] × 100
From Fig. 7, table 6 as it can be seen that biocontrol bacterial strain FJ17-4 bacteria suspension (biocontrol agent) and 58% metalaxyl Mancozeb are wettable
Property pulvis 700 there is certain control efficiency to the capsicum epidemic disease in (crop field) again, control efficiency is respectively 80.31% and 74.5%,
The control efficiency of FJ17-4 bacterial strain bacteria suspension (biocontrol agent) is higher than 700 times of 58% metalaxyl mancozeb wettable powder
Control efficiency shows preferable application prospect.
Field control effect of the 6 Bei Laisi bacillus FJ17-4 bacteria suspension of table to capsicum epidemic disease
SEQUENCE LISTING
<110>Inst. of Plant Protection, fujian Academy of Agricultural Science
Application of<120>one plants of Bei Laisi bacillus in prevention and treatment capsicum epidemic disease
<130> 1
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1447
<212> DNA
<213>16s rDNA sequence
<400> 1
gggcggcgtg cctatacatg cagtcgagcg gacagatggg agcttgctcc ctgatgttag 60
cggcggacgg gtgagtaaca cgtgggtaac ctgcctgtaa gactgggata actccgggaa 120
accggggcta ataccggatg gttgtttgaa ccgcatggtt cagacataaa aggtggcttc 180
ggctaccact tacagatgga cccgcggcgc attagctagt tggtgaggta acggctcacc 240
aaggcgacga tgcgtagccg acctgagagg gtgatcggcc acactgggac tgagacacgg 300
cccagactcc tacgggaggc agcagtaggg aatcttccgc aatggacgaa agtctgacgg 360
agcaacgccg cgtgagtgat gaaggttttc ggatcgtaaa gctctgttgt tagggaagaa 420
caagtgccgt tcaaataggg cggcaccttg acggtaccta accagaaagc cacggctaac 480
tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggaat tattgggcgt 540
aaagggctcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600
gtcattggaa actggggaac ttgagtgcag aagaggagag tggaattcca cgtgtagcgg 660
tgaaatgcgt agagatgtgg aggaacacca gtggcgaagg cgactctctg gtctgtaact 720
gacgctgagg agcgaaagcg tggggagcga acaggattag ataccctggt agtccacgcc 780
gtaaacgatg agtgctaagt gttagggggt ttccgcccct tagtgctgca gctaacgcat 840
taagcactcc gcctggggag tacggtcgca agactgaaac tcaaaggaat tgacgggggc 900
ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960
ttgacatcct ctgacaatcc tagagatagg acgtcccctt cgggggcaga gtgacaggtg 1020
gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca 1080
acccttgatc ttagttgcca gcattcagtt gggcactcta aggtgactgc cggtgacaaa 1140
ccggaggaag gtggggatga cgtcaaatca tcatgcccct tatgacctgg gctacacacg 1200
tgctacaatg gacagaacaa agggcagcga aaccgcgagg ttaagccaat cccacaaatc 1260
tgttctcagt tcggatcgca gtctgcaact cgactgcgtg aagctggaat cgctagtaat 1320
cgcggatcag catgccgcgg tgaatacgtt cccgggcctt gtacacaccg cccgtcacac 1380
cacgagagtt tgtaacaccc gaagtcggtg aggtaacctt taggagccag ccgccgaagt 1440
ggaccta 1447
Claims (2)
1. application of one plant of Bei Laisi bacillus in prevention and treatment capsicum epidemic disease, the bacterium are Bei Laisi bacillus
(Bacillusvelezensis) FJ17-4, China Committee for Culture Collection of Microorganisms was preserved on September 18th, 2017
Common micro-organisms center, deposit number are CGMCC No.14642.
2. application according to claim 1, it is characterised in that: by the Bei Laisi bacillus
(Bacillusvelezensis) FJ17-4 is prepared into biocontrol agent, preparation method be by FJ17-4 28 DEG C in LB culture solution,
24 h of 180rpm shaken cultivation, then 4000 rpm are centrifuged 10 min, collect the bacterial strain in precipitating, are carried out bacterial strain with aqua sterilisa
It is 1 × 10 that viable bacteria concentration, which is made, in dilution9-1×1010The bacterium suspension of CFU/mL.
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| CN108753664B (en) * | 2018-06-28 | 2021-03-26 | 广东省农业科学院农业资源与环境研究所 | Biological control agent, biological organic fertilizer and application thereof |
| CN109055281B (en) * | 2018-09-19 | 2021-08-24 | 北京化工大学 | Bacillus belgii ZF2 and application thereof in plant disease control |
| CN111073825B (en) * | 2018-10-19 | 2022-07-12 | 沈阳药科大学 | Bacterium with plant soil-borne disease resistance effect and application thereof |
| CN110184221B (en) * | 2019-06-05 | 2021-03-05 | 中国农业科学院蔬菜花卉研究所 | Bacillus belgii and application thereof in prevention and treatment of phytophthora root rot of tomato |
| CN110564646B (en) * | 2019-09-20 | 2020-05-05 | 湖北省生物农药工程研究中心 | Bacillus belgii CY30 and application thereof in prevention and treatment of tea round spot |
| CN115029269B (en) * | 2022-06-21 | 2024-01-30 | 新疆农业大学 | Lipopeptid antibiotic-producing erwinia amylovora antagonistic bacterium, and fermentation method and application thereof |
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| KR101089149B1 (en) * | 2008-11-18 | 2011-12-02 | 한국화학연구원 | Bacillus velezensis s3-5 strain and method for the biological control of plant diseases using same |
| KR101190650B1 (en) * | 2010-07-23 | 2012-10-17 | 대한민국 | A novel Bacillus velezensis B-42 for multi function |
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