CN108029602A - A kind of method for preventing stichopus japonicus from producing stress reaction - Google Patents
A kind of method for preventing stichopus japonicus from producing stress reaction Download PDFInfo
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- CN108029602A CN108029602A CN201711450467.6A CN201711450467A CN108029602A CN 108029602 A CN108029602 A CN 108029602A CN 201711450467 A CN201711450467 A CN 201711450467A CN 108029602 A CN108029602 A CN 108029602A
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- stichopus japonicus
- menthol
- stress reaction
- japonicus
- water body
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- 241000965254 Apostichopus japonicus Species 0.000 title claims abstract description 261
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 43
- NOOLISFMXDJSKH-UTLUCORTSA-N (+)-Neomenthol Chemical compound CC(C)[C@@H]1CC[C@@H](C)C[C@@H]1O NOOLISFMXDJSKH-UTLUCORTSA-N 0.000 claims abstract description 75
- NOOLISFMXDJSKH-UHFFFAOYSA-N DL-menthol Natural products CC(C)C1CCC(C)CC1O NOOLISFMXDJSKH-UHFFFAOYSA-N 0.000 claims abstract description 75
- 229940041616 menthol Drugs 0.000 claims abstract description 75
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 63
- 230000005059 dormancy Effects 0.000 claims abstract description 44
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 20
- 238000011084 recovery Methods 0.000 claims description 39
- 238000009395 breeding Methods 0.000 claims description 26
- 230000001488 breeding effect Effects 0.000 claims description 26
- 239000007864 aqueous solution Substances 0.000 claims description 10
- 241000208340 Araliaceae Species 0.000 claims description 4
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 4
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 4
- 235000008434 ginseng Nutrition 0.000 claims description 4
- 230000000284 resting effect Effects 0.000 abstract description 15
- 230000000638 stimulation Effects 0.000 abstract description 4
- 238000011017 operating method Methods 0.000 abstract description 3
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract description 2
- 230000035882 stress Effects 0.000 description 30
- 230000032258 transport Effects 0.000 description 28
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 26
- 230000004083 survival effect Effects 0.000 description 25
- 239000013078 crystal Substances 0.000 description 17
- 244000246386 Mentha pulegium Species 0.000 description 12
- 235000016257 Mentha pulegium Nutrition 0.000 description 12
- 235000004357 Mentha x piperita Nutrition 0.000 description 12
- 235000001050 hortel pimenta Nutrition 0.000 description 12
- 230000000052 comparative effect Effects 0.000 description 9
- 238000004090 dissolution Methods 0.000 description 6
- 238000007654 immersion Methods 0.000 description 6
- 239000013535 sea water Substances 0.000 description 6
- 210000000936 intestine Anatomy 0.000 description 5
- 238000009287 sand filtration Methods 0.000 description 5
- 235000019750 Crude protein Nutrition 0.000 description 4
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 4
- 239000004472 Lysine Substances 0.000 description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 4
- 235000019784 crude fat Nutrition 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 229910052698 phosphorus Inorganic materials 0.000 description 4
- 239000011574 phosphorus Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 206010002091 Anaesthesia Diseases 0.000 description 2
- 241000251511 Holothuroidea Species 0.000 description 2
- 230000037005 anaesthesia Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000004938 stress stimulation Effects 0.000 description 2
- CUJMCPPBTUATEJ-UHFFFAOYSA-N 1-(3-methyl-4-oxido-1-oxoquinoxalin-1-ium-2-yl)ethanone Chemical compound C1=CC=C2[N+](=O)C(C(=O)C)=C(C)N([O-])C2=C1 CUJMCPPBTUATEJ-UHFFFAOYSA-N 0.000 description 1
- 206010053759 Growth retardation Diseases 0.000 description 1
- 201000003176 Severe Acute Respiratory Syndrome Diseases 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000001949 anaesthesia Methods 0.000 description 1
- 230000003444 anaesthetic effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000009429 distress Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 231100000001 growth retardation Toxicity 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000003116 impacting effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/30—Culture of aquatic animals of sponges, sea urchins or sea cucumbers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to aquaculture technical field, provide a kind of method for preventing stichopus japonicus from producing stress reaction, dormancy agent menthol is put into apostichopus japonicus culture water body, the menthol quality of every cubic metre of apostichopus japonicus culture water body input is 2.5~100g, after more than 1/2 stichopus japonicus in physical relaxation extended position or more than 1/2 stichopus japonicus from attachment base disengaging after, carry out manual operation；Corresponding dormancy agent amount of application is provided according to the weight specification difference of stichopus japonicus.The present invention is before it may result in the manual operation of stichopus japonicus stress reaction, dormancy is carried out to stichopus japonicus using menthol, resting state will not cause stichopus japonicus to produce stress reaction, carrying out manual operation in the dormant state at the same time will not make stichopus japonicus produce stress reaction, that is, avoids stichopus japonicus and the possibility of stress reaction occur.Method provided by the invention broken the prior art with reduce manual operation intensity, simplify operating procedure, control stimulation time come avoid stichopus japonicus occur stress reaction conventional thought.
Description
Technical field
The present invention relates to aquaculture technical field, and in particular to a kind of method for preventing stichopus japonicus from producing stress reaction.
Background technology
Since ancient times, stichopus japonicus just enjoys people to favor with its unrivaled unique nutritional health function, especially from 2003
Since year Severe Acute Respiratory Syndromes event outburst, apostichopus japonicus culture industry is rapidly expanded, and becomes one of pillar industry of Chinese sea-farming.
In the production process of stichopus japonicus, the manual operations such as pond, clear bottom, replacement attachment base, stichopus japonicus parent and seed transport of falling are must not
The important step that can lack, but above-mentioned manual operation can impact stichopus japonicus, stichopus japonicus gave birth to originally because of different degrees of disengaging
Habitat living and produce stress reaction.The stress reaction of stichopus japonicus be easy to cause stichopus japonicus stress it is dead, spit intestines and late growth
The problem of slow.Therefore, how to ensure stichopus japonicus in various artificial post-stimulatory survival rates, avoid impacting subsequent growth,
The bottleneck problem of urgent need to resolve always in this area.
At present, it has been disclosed that stichopus japonicus live body preserve, in transport and the technical data such as the patent of post-stress recovery and document,
For by controlling water temperature, oxygenation, medication and shortening stimulation time etc. because usually realizing, specific effect is also needed depending on stichopus japonicus
Itself constitution, no matter which kind of method, its principle mainly ensures the basic survival condition of stichopus japonicus using exterior artificial means, reduces people
Work manipulation strength, shorten the operating time to achieve the purpose that to prevent stress reaction.These methods are subject to dissolved oxygen amount, water temperature, transport
The external factor such as time, stichopus japonicus constitution have a great influence, and technique is cumbersome, the ineffective and guarantee time-to-live is shorter, can not be complete
The stress reaction that manual operation produces stichopus japonicus is eliminated, also easily causes stichopus japonicus resistance to decline after manual operation, fall ill, is dead
Die, spit intestines and the adverse consequences such as slow-growing, influence the productivity effect of apostichopus japonicus culture.
The content of the invention
In view of this, it is an object of the invention to provide a kind of method for preventing stichopus japonicus from producing stress reaction, stichopus japonicus is made
Growth and development from stress stimulation influence.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：
The present invention provides a kind of method for preventing stichopus japonicus from producing stress reaction, and dormancy agent menthol is mixed bait input thorn
Join breeding water body, the menthol quality of every cubic metre of apostichopus japonicus culture water body input is 2.5~100g, and the stichopus japonicus for treating more than 1/2 is in
Physical relaxation extended position or more than 1/2 stichopus japonicus carry out manual operation after attachment base disengaging；
When the stichopus japonicus specification contained in every cube of breeding water body is less than 5000/jin, the input amount of the menthol is
2.5~5g/m3；
When the stichopus japonicus specification contained in every cube of breeding water body is 1000~5000/jin, the input of the menthol
Measure as 5.5~10g/m3；
When the stichopus japonicus specification contained in every cube of breeding water body is 100~999/jin, the input of the menthol
Measure as 10.5~25g/m3；
When the stichopus japonicus specification contained in every cube of breeding water body is 20~99/jin, the input amount of the menthol is
25.5~50g/m3；
When the stichopus japonicus specification contained in every cube of breeding water body is more than 20/jin, the input amount of the menthol is
50.5~100g/m3。
Preferably, the manual operation includes replacing attachment base, pond, sieve seedling or transport.
Preferably, the dormancy agent menthol is mixed bait input water body by 1.5~3h before manual operation.
Preferably, the stichopus japonicus of the dormancy agent menthol input water body to more than 1/2 is in physical relaxation extended position or 1/2
Stichopus japonicus above is 15~40min from the time after attachment base disengaging.
Preferably, the mass ratio of the menthol and bait is 1~2:3~16.
Preferably, the stichopus japonicus gross mass in every cubic metre of breeding water body and the mass ratio of the bait are (80~120):(2
~10).
Preferably, the handling time is no longer than 16h.
Preferably, after the manual operation, the step of further including recovery stichopus japonicus；The method of the recovery stichopus japonicus includes：Will
The stichopus japonicus of dormancy soaks 45~60min in brown sugar aqueous solution, you can recovery stichopus japonicus.
Preferably, the concentration of the brown sugar aqueous solution is 100~200mg/L.
Preferably, do not fed in 10~14h after the stichopus japonicus recovery, feed thrown according to the 0.5~2% of stichopus japonicus weight first
Bait is fed, recovers normal feeding volume after recovery after 24~36h again.
Compared with prior art, the present invention the present invention has the following advantages：
Method provided by the invention is that dormancy agent menthol is put into apostichopus japonicus culture water body, every cubic metre of apostichopus japonicus culture water body
The menthol quality of input is 2.5~100g, and the stichopus japonicus for treating more than 1/2 is in physical relaxation extended position or more than 1/2 stichopus japonicus
After attachment base disengaging, manual operation is carried out；Corresponding dormancy agent amount of application is provided according to the weight specification difference of stichopus japonicus.This
Invention carries out dormancy before it may result in the manual operation of stichopus japonicus stress reaction, using menthol to stichopus japonicus, and resting state is not
Stichopus japonicus can be caused to produce stress reaction, while carrying out manual operation in the dormant state will not make stichopus japonicus produce stress reaction,
Avoid stichopus japonicus and the possibility of stress reaction occur.Method provided by the invention has broken the prior art to reduce manual operation
Intensity, the operating procedure that simplifies, control stimulation time to avoid stichopus japonicus that the conventional thought of stress reaction occurs.There is provided using the present invention
The survival rate of Apostichopus japonicus of method processing can reach 100%, and thoroughly solve stichopus japonicus stress be anti-even across conventional reduction
Should handle still occur it is slow-growing, spit the problem of stress reactions such as intestines.
The present invention is safe and harmless to stichopus japonicus, stimulate small, duration length using menthol as dormancy agent, and resting state can be held
Continuous 8~12h, plenty of time is provided for stichopus japonicus live transport, the manual operations such as pond, replacement attachment base of falling.Especially for thorn
Ginseng live transport provide it is longer can haulage time, the live bodies of existing stichopus japonicus live transport means can haulage time for 3~
4h, using it is of the present invention prevent stichopus japonicus produce stress reaction method can make live body can haulage time extend more than one times.
Menthol using 2.5~100g of input in every cubic metre of breeding water body the stopping as stichopus japonicus of the invention
Dormancy agent, without making stichopus japonicus reach deep anaesthesia state, keeps resting state.The discovery of the invention makes thorn for a long time
External world's manual operation will not cause stichopus japonicus stress reaction occur when ginseng enters resting state, and cost is low, easy to operate.
Embodiment
The present invention provides a kind of method for preventing stichopus japonicus from producing stress reaction, and dormancy agent menthol is mixed bait input thorn
Join breeding water body, the menthol quality of every cubic metre of apostichopus japonicus culture water body input is 2.5~100g, and the stichopus japonicus for treating more than 1/2 is in
Physical relaxation extended position or more than 1/2 stichopus japonicus carry out manual operation, carry out manual operation after attachment base disengaging；
When the stichopus japonicus specification contained in every cube of breeding water body is less than 5000/jin, the input amount of the menthol is
2.5~5g/m3；
When the stichopus japonicus specification contained in every cube of breeding water body is 1000~5000/jin, the input of the menthol
Measure as 5.5~10g/m3；
When the stichopus japonicus specification contained in every cube of breeding water body is 100~999/jin, the input of the menthol
Measure as 10.5~25g/m3；
When the stichopus japonicus specification contained in every cube of breeding water body is 20~99/jin, the input amount of the menthol is
25.5~50g/m3；
When the stichopus japonicus specification contained in every cube of breeding water body is more than 20/jin, the input amount of the menthol is
50.5~100g/m3。
In the present invention, the menthol is the dormancy agent of stichopus japonicus, and stichopus japonicus can be made to enter shallow-layer resting state, reduced
The sensation and the consciousness of motion of stichopus japonicus, but and need not can be achieved to avoid the work of stichopus japonicus generation stress reaction into deep layer anesthesia
With.
In the present invention, the manual operation includes replacing attachment base, pond, sieve seedling or transport.
Dormancy agent is mixed bait input apostichopus japonicus culture water body by currently preferred 1.5~3h before manual operation, more preferably
For 2h before manual operation.
Dormancy agent menthol is mixed bait input apostichopus japonicus culture water body, often by the present invention according to the difference of stichopus japonicus size specification
The menthol quality of cubic meter apostichopus japonicus culture water body input is 2.5~100g；Treat that more than 1/2 stichopus japonicus unfolds shape in physical relaxation
State or more than 1/2 stichopus japonicus carry out manual operation after attachment base disengaging.The stichopus japonicus for occurring more than 1/2 when stichopus japonicus is put in body
During the phenomenon that loose extended position or more than 1/2 stichopus japonicus depart from from attachment base, show that stichopus japonicus comes into resting state.Such a shape
Under state carry out manual operation will not be perceived by stichopus japonicus, into without make stichopus japonicus produce the stress reaction caused by environmental stimuli, from
Fundamentally thoroughly prevent the stress reaction of stichopus japonicus to occur, broken conventional reduction manual operation intensity, simplify operating procedure,
Stimulation time is controlled to avoid stichopus japonicus that the conventional thought of stress reaction occurs.
In the present invention, stichopus japonicus more it is big it is then per jin in stichopus japonicus head number it is smaller, the stichopus japonicus specification is by containing in per jin
Some stichopus japonicus numbers represents the Individual Size of stichopus japonicus, according to the difference of stichopus japonicus size, can make it into resting state and not
The menthol concentration that producing stimulates is adjusted accordingly, i.e., as the dosage of the increase menthol of stichopus japonicus quality also increases accordingly
Add, but menthol is not proportional with stichopus japonicus quality, thus the invention is true to a certain range of stichopus japonicus quality
The dosage of corresponding dormancy agent menthol is determined.
In the present invention, dormancy agent menthol input water body to the stichopus japonicus for treating more than 1/2 unfolds shape in physical relaxation
The time that state or more than 1/2 stichopus japonicus depart from from attachment base is 15~40min, represents that stichopus japonicus enters resting state at this time；Foundation
The dosage of different size and menthol, the time that stichopus japonicus enters resting state is different, in method of the present invention：
When the stichopus japonicus specification contained in every cube of breeding water body is less than 5000/jin, the input amount of the menthol is
2.5~5g/m3, the time into dormancy is 5~10min, and the dormancy duration is 8~10h；
When the stichopus japonicus specification contained in every cube of breeding water body is 1000~5000/jin, the input of the menthol
Measure as 5.5~10g/m3, the time into dormancy is 10~20min, and the dormancy duration is 8~10h；
When the stichopus japonicus specification contained in every cube of breeding water body is 100~999/jin, the input of the menthol
Measure as 10.5~25g/m3, the time into dormancy is 15~20min, and the dormancy duration is 8~10h；
When the stichopus japonicus specification contained in every cube of breeding water body is 20~99/jin, the input amount of the menthol is
25.5~50g/m3, the time into dormancy is 20~30min, and the dormancy duration is 10~16h；
When the stichopus japonicus specification contained in every cube of breeding water body is more than 20/jin, the input amount of the menthol is
50.5~100g/m3, the time into dormancy is 30~40min, and the dormancy duration is 10~16h.
In the present invention, the mass ratio of the dormancy agent menthol and bait is preferably 1~2:3~16, more preferably 1:6
~16.In the present invention, in every cubic metre of apostichopus japonicus culture water body, stichopus japonicus gross mass and the mass ratio of the bait are preferably
(80~120):(2~10), more preferably 100:3~5.The present invention is by the way of dormancy agent mixes bait input, a part of peppermint
Alcohol can feed bait into playing a role in vivo by stichopus japonicus, can effectively shorten the time that stichopus japonicus enters dormancy, improve
Efficiency.
Specifically, the method that menthol of the present invention mixes bait is：Use ethanol by menthol dissolution of crystals for solution,
Mixed with bait.Bait of the present invention is preferably the mixed feed of stichopus japonicus.The present invention is to the menthol and bait
Source be not particularly limited, using commercial goods.In the present invention, during ethanol dissolving peppermint alcohol crystals, what is played is to help
The dosage of molten effect, i.e. ethanol can be by menthol dissolution of crystals.
In the present invention, the manually-operated time is no longer than 16h, stops stichopus japonicus using the method for the invention
The time of abundance is provided after dormancy for manual operation.Especially when manual operation is transports stichopus japonicus, method provided by the invention
The stichopus japonicus live transport time of 8~16h is capable of providing, is prior art transport 2 times of the live body stichopus japonicus time, survival rate of Apostichopus japonicus
Height, and stichopus japonicus is not in spit the stress reactions such as intestines, growth retardation after breaking dormancy.
Prevent stichopus japonicus from the method for stress reaction occur of the present invention, water temperature is 10~25 DEG C.
In the present invention, the method for preventing that stichopus japonicus from producing stress reaction further includes following recovery thorn after manual operation
The step of ginseng：The stichopus japonicus of dormancy is soaked into 45~60min in sugar aqueous solution, the stichopus japonicus of dormancy is recovered.Preferably, during immersion
Between be 30min.
It is as successfully multiple when the body and quil for observing stichopus japonicus are stretched, gradually swashed to attachment base or pool wall naturally
Soviet Union.The present invention contributes to stichopus japonicus to regain one's strength rapidly, while improve the immunity of organism of stichopus japonicus using brown sugar water immersion stichopus japonicus recovery
Power, avoids or prevents since distress reaction occurs caused by the manual operation of long period.
In the present invention, the mass-volume concentration of the brown sugar aqueous solution is preferably 100~200mg/L, and more preferably 120
~180mg/L.Preferably, brown sugar aqueous solution of the present invention is using sand filtration seawater as solvent.Brown sugar aqueous solution of the present invention is excellent
Elect brown sugar aqueous solution as.
The stichopus japonicus after sugar aqueous solution soaks recovers from dormancy, 10~14h of the present invention after stichopus japonicus recovers
It is interior 0.5~2% to feed bait according to stichopus japonicus weight when feeding first without feeding, it is extensive again after 24~36h after recovery
Multiple normal feeding volume.
In the present invention, it is preferred to stichopus japonicus recover after 12~13h in without feeding.After not feeding, this hair
It is bright preferably to feed bait according to the 1% of stichopus japonicus weight when feeding first.The time of feeding volume back to normal of the present invention
30h preferably after stichopus japonicus recovery.
With reference to the embodiment of the present invention, the technical solution in the present invention is clearly and completely described.Based on this
Embodiment in invention, all other reality that those of ordinary skill in the art are obtained without making creative work
Example is applied, belongs to the scope of protection of the invention.
Embodiment 1 prevents the influence transported for long-distance to stichopus japonicus
In 10 cubic metres of water body, per cubic meter of water contains 2 jin of stichopus japonicus, and the specification of stichopus japonicus is 2000/jin.Preparing
Carry out transporting preceding 2h, mixed feed 300g is weighed according to the 3% of stichopus japonicus weight, according to mixed feed and menthol mass ratio 3:1
Ratio weigh peppermint alcohol crystals 100g, will be mixed with a small amount of ethanol after menthol dissolution of crystals with mixed feed, by mixture
Put into water body.It is when more than 1/2 stichopus japonicus departs from physical relaxation extended position or more than 1/2 stichopus japonicus from attachment base
Resting state is entered, can carry out live transport to the stichopus japonicus after dormancy at this time.
After transport, stichopus japonicus is placed in clean sand filtration seawater, the brown sugar of 100~200mg/L of addition, immersion 20~
45min, more than 1/2 stichopus japonicus recover normal condition (body and quil stretch naturally, gradually swash to attachment base or pool wall), stichopus japonicus
Successful resuscitation, measures the survival rate of stichopus japonicus at this time.
Any bait is not fed in 12h after stichopus japonicus recovery, when the 12h after stichopus japonicus recovery is fed first, according to stichopus japonicus
The 1% of weight feeds mixed feed, is gradually increased feeding volume, until 24h recovers normal feeding volume again after recovery.The of recovery
7d measures the survival rate of stichopus japonicus；Stichopus japonicus specific growth rate (SGR) is measured during the 30d of recovery.
Wherein, in Qingdao Lang Ge Bioisystech Co., Ltd, Major Nutrient, which forms, is for mixed feed purchase：Crude protein
16%th, crude fat 5%, crude fibre 8%, total phosphorus 6%, coarse ash 25%, lysine 0.8%, moisture 10%.
The conventional method for reducing stichopus japonicus stress reaction of comparative example 1
In 10 cubic metres of water body, per cubic meter of water contains 2 jin of stichopus japonicus, and the specification of stichopus japonicus is 2000/jin.
(1) temporarily support on an empty stomach：The stichopus japonicus for being ready for transport is stopped into bait 2 days, makes its interior residue that empties the intestine.
(2) it is oxygenated：The container of temporary cultivating sea cucumber is oxygenated using oxygen charging pump.
(3) cool down：Be slowly added to seawater ice cube or sealing ice chest cooling in the water of temporary cultivating sea cucumber, control water temperature 8~
Between 15 DEG C.
(4) pack：Temporarily foster stichopus japonicus is fitted into foam insulation box and carries out dry fortune or water transport, needs to exist again before incubator sealing
Appropriate sealing ice chest is put into case to keep low temperature (8~15 DEG C).
(5) transport：Route planning is made, ensures vehicle, personnel etc. in place, shortens the time as far as possible.
(6) recover：After manual operation completion or transport arrive at, using resisting stress class medicine (such as mequindox)
To prevent the influence to stichopus japonicus, more than 1/2 stichopus japonicus recovers normal condition, and (body and quil stretch naturally, gradually swash to attachment
Base or pool wall) when measure survival rate of Apostichopus japonicus.
Embodiment 2
Survival rate carries out after this experiment improves stichopus japonicus manual operation to the method for preventing stichopus japonicus from producing stress reaction
Verification.
According to the method for embodiment 1 or comparative example 1 to stichopus japonicus carry out live transport, the live transport time be followed successively by 2h, 4h,
6h、8h、10h、12h、16h；The survival rate after stichopus japonicus recovers normal is detected respectively, and the results are shown in Table 1：
The stichopus japonicus live transport time of 1 distinct methods of table and survival rate
It can be seen from the data of table 1 compared with comparative example 1, the survival rate of Apostichopus japonicus of 1 method of embodiment transport significantly carries
Height, and the method transport stichopus japonicus of embodiment 1 has reached 100% in 2~10h, and 1 the method for comparative example transports stichopus japonicus
Survival rate be only up to 99%.Show that the method provided by the invention for preventing that stichopus japonicus from producing stress reaction can significantly improve people
Survival rate of Apostichopus japonicus after work operation；The conventional stichopus japonicus live transport time is in 2~8h, stichopus japonicus live transport provided by the invention
Between can reach 16h, i.e. 1 times of live transport time lengthening.
Embodiment 3 prevents down influence of the pond to stichopus japonicus
In 10 cubic metres of water body, per cubic meter of water contains 2 jin of stichopus japonicus, and the specification of stichopus japonicus is 2000/jin.Preparing
Carry out transporting preceding 2h, mixed feed 300g is weighed according to the 3% of stichopus japonicus weight, according to mixed feed and menthol mass ratio 3:1
Ratio weigh peppermint alcohol crystals 100g, will be mixed with a small amount of ethanol after menthol dissolution of crystals with mixed feed, by mixture
Put into water body.Treat more than 1/2 stichopus japonicus in physical relaxation extended position or more than 1/2 stichopus japonicus from attachment base disengaged position
When be enter resting state, can carry out down pond to the stichopus japonicus after dormancy at this time.
Behind pond, stichopus japonicus is placed in clean sand filtration seawater, the brown sugar of 100~200mg/L of addition, immersion 20~
45min, more than 1/2 stichopus japonicus recover normal condition (body and quil stretch naturally, gradually swash to attachment base or pool wall), stichopus japonicus
Successful resuscitation, measures the survival rate of stichopus japonicus at this time.
Any bait is not fed in 12h after stichopus japonicus recovery, when the 12h after stichopus japonicus recovery is fed first, according to stichopus japonicus
The 1% of weight feeds mixed feed, is gradually increased feeding volume, until 24h recovers normal feeding volume again after recovery.Recovery
7d measures the survival rate of stichopus japonicus；Stichopus japonicus specific growth rate (SGR) is measured during the 30d of recovery.
Wherein, in Qingdao Lang Ge Bioisystech Co., Ltd, Major Nutrient, which forms, is for mixed feed purchase：Crude protein
16%th, crude fat 5%, crude fibre 8%, total phosphorus 6%, coarse ash 25%, lysine 0.8%, moisture 10%.
Embodiment 4
Except according to mixed feed and menthol mass ratio 6:1 ratio is weighed outside peppermint alcohol crystals 50g, other steps and reality
It is identical to apply example 3.
Embodiment 5
Except according to mixed feed and menthol mass ratio 3:2 ratio is weighed outside peppermint alcohol crystals 200g, other steps with
Embodiment 3 is identical.
Comparative example 2
In addition to menthol is not added, other steps are same as Example 3.
Embodiment 6
In 10 cubic metres of water body, per cubic meter of water contains 6 jin of stichopus japonicus, and the specification of stichopus japonicus is 200/jin.Preparing
Carry out transporting preceding 1.5h, mixed feed 1500g is weighed according to the 5% of stichopus japonicus weight, according to mixed feed and menthol mass ratio
12:1 ratio weighs peppermint alcohol crystals 125g, will be mixed with a small amount of ethanol after menthol dissolution of crystals with mixed feed, will be mixed
In compound input water body.When more than 1/2 stichopus japonicus departs from physical relaxation extended position or more than 1/2 stichopus japonicus from attachment base
Resting state is as entered, can carry out down pond to the stichopus japonicus after dormancy at this time.
Behind pond, stichopus japonicus is placed in clean sand filtration seawater, the brown sugar of 100~200mg/L of addition, immersion 20~
45min, more than 1/2 stichopus japonicus recover normal condition (body and quil stretch naturally, gradually swash to attachment base or pool wall), stichopus japonicus
Successful resuscitation, measures the survival rate of stichopus japonicus at this time.
Any bait is not fed in 10h after stichopus japonicus recovery, when the 11h after stichopus japonicus recovery is fed first, according to stichopus japonicus
The 0.5% of weight feeds mixed feed, is gradually increased feeding volume, until 24h recovers normal feeding volume again after recovery.Recovery
7d measure stichopus japonicus survival rate；Stichopus japonicus specific growth rate (SGR) is measured during the 30d of recovery.
Wherein, in Qingdao Lang Ge Bioisystech Co., Ltd, Major Nutrient, which forms, is for mixed feed purchase：Crude protein
16%th, crude fat 5%, crude fibre 8%, total phosphorus 6%, coarse ash 25%, lysine 0.8%, moisture 10%.
Embodiment 7
Except according to mixed feed and menthol mass ratio 8:1 ratio is weighed outside peppermint alcohol crystals 187.5g, remaining step
It is same as Example 6.
Embodiment 8
Except according to mixed feed and menthol mass ratio 6:1 ratio is weighed outside peppermint alcohol crystals 250g, remaining step is equal
It is same as Example 6.
Comparative example 3
In addition to menthol is not added, remaining step is same as Example 6.
Embodiment 9
In 10 cubic metres of water body, per cubic meter of water contains 10 jin of stichopus japonicus, and the specification of stichopus japonicus is 10/jin.Preparing
Carry out transporting preceding 1.5h, mixed feed 5000g is weighed according to the 5% of stichopus japonicus weight, according to mixed feed and menthol mass ratio
8:1 ratio weighs peppermint alcohol crystals 625g, will be mixed, will mixed with mixed feed after menthol dissolution of crystals with a small amount of ethanol
In thing input water body.Treat that stichopus japonicus occurs that body is rolled up, more than 1/2 quil is shunk, adhesive force declines the state such as (more than 1/2 comes off)
When be enter resting state, at this time can to after dormancy stichopus japonicus carry out live transport.
After transport, stichopus japonicus is placed in clean sand filtration seawater, the brown sugar of 100~200mg/L of addition, immersion 20~
45min, more than 1/2 stichopus japonicus recover normal condition (body and quil stretch naturally, gradually swash to attachment base or pool wall), stichopus japonicus
Successful resuscitation, measures the survival rate of stichopus japonicus at this time.
Any bait is not fed in 14h after stichopus japonicus recovery, when the 15h after stichopus japonicus recovery is fed first, according to stichopus japonicus
The 0.5% of weight feeds mixed feed, is gradually increased feeding volume, until recovering normal feeding volume again within the 30th day after recovery.It is multiple
The survival rate of the 7d measure stichopus japonicus of Soviet Union；Stichopus japonicus specific growth rate (SGR) is measured during the 30d of recovery.
Wherein, in Qingdao Lang Ge Bioisystech Co., Ltd, Major Nutrient, which forms, is for mixed feed purchase：Crude protein
16%th, crude fat 5%, crude fibre 8%, total phosphorus 6%, coarse ash 25%, lysine 0.8%, moisture 10%.
Embodiment 10
Except according to mixed feed and menthol mass ratio 6.25:1 ratio is weighed outside peppermint alcohol crystals 800g, remaining step
With embodiment 9.
Embodiment 11
Except according to mixed feed and menthol mass ratio 5:1 ratio is weighed outside peppermint alcohol crystals 1000g, remaining step is equal
With embodiment 9.
Comparative example 4
In addition to menthol is not added, remaining step is same as Example 9.
Embodiment 12
Survival rate of Apostichopus japonicus, stichopus japonicus specific growth rate SGR after the recovery that Statistics Implementation example 3~11, comparative example 2~4 detect, tool
Volume data is shown in Table 2.
Recovery time, survival rate and the specific growth rate of the stichopus japonicus of 2 different size of table after treatment
It can be seen that according to the data of table 2 compared with comparative example, method provided in an embodiment of the present invention can be carried effectively
Survival rate after high stichopus japonicus recovery, the recovery time is short, and the specific growth rate after recovery is also opposite to be improved, this shows offer of the present invention
Method can effectively improve the survival rate of stichopus japonicus, and on the late growth speed of stichopus japonicus without influence.
It can also be seen that under same stichopus japonicus specification, survival rate and specific life with the rise stichopus japonicus of menthol concentration
Long rate declines therewith, this shows that the higher menthol of concentration has still influenced stichopus japonicus specific growth rate and survival rate, this hair
The method of bright offer enters resting state by relatively low menthol concentration stichopus japonicus, both can be to avoid manual operation pair
The stress stimulation that stichopus japonicus produces, to the influence of stichopus japonicus late growth speed when being also possible to prevent menthol as anesthetic.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
- A kind of 1. method for preventing stichopus japonicus from producing stress reaction, it is characterised in that dormancy agent menthol is mixed into bait input stichopus japonicus Breeding water body, the menthol quality of every cubic metre of apostichopus japonicus culture water body input is 2.5~100g, and the stichopus japonicus for treating more than 1/2 is in body Body loosens extended position or more than 1/2 stichopus japonicus after attachment base disengaging, carries out manual operation；When the stichopus japonicus specification contained in every cube of breeding water body is less than 5000/jin, the input amount of the menthol for 2.5~ 5g/m3；When the stichopus japonicus specification contained in every cube of breeding water body is 1000~5000/jin, the input amount of the menthol is 5.5~10g/m3；When the stichopus japonicus specification contained in every cube of breeding water body is 100~999/jin, the input amount of the menthol is 10.5~25g/m3；When the stichopus japonicus specification contained in every cube of breeding water body is 20~99/jin, the input amount of the menthol is 25.5 ~50g/m3；When the stichopus japonicus specification contained in every cube of breeding water body is more than 20/jin, the input amount of the menthol for 50.5~ 100g/m3。
- 2. the method for preventing stichopus japonicus from producing stress reaction according to claim 1, it is characterised in that the manual operation includes Replace attachment base, pond, sieve seedling or transport.
- 3. the method for preventing stichopus japonicus from producing stress reaction according to claim 1, it is characterised in that 1.5 before manual operation The dormancy agent menthol is mixed bait input water body by~3h.
- 4. the method for preventing stichopus japonicus from producing stress reaction according to claims 1 to 3 any one, it is characterised in that described The stichopus japonicus of dormancy agent menthol input water body to more than 1/2 is in physical relaxation extended position or more than 1/2 stichopus japonicus from attachment base Time after disengaging is 15~40min.
- 5. the method for preventing stichopus japonicus from producing stress reaction according to claims 1 to 3 any one, it is characterised in that described The mass ratio of menthol and bait is 1~2:3~16.
- 6. the method for preventing stichopus japonicus from producing stress reaction according to claim 5, it is characterised in that every cubic metre of breeding water body In the mass ratio of stichopus japonicus gross mass and the bait be (80~120):(2~10).
- 7. the method for preventing stichopus japonicus from producing stress reaction according to claims 1 to 3 any one, it is characterised in that described Handling time is no longer than 16h.
- 8. the method for preventing stichopus japonicus from producing stress reaction according to claims 1 to 3 any one, it is characterised in that described After manual operation, the step of further including recovery stichopus japonicus；The method of the recovery stichopus japonicus includes：The stichopus japonicus of dormancy is soaked into 45~60min in brown sugar aqueous solution, you can recovery thorn Ginseng.
- 9. the method for preventing stichopus japonicus from producing stress reaction according to claim 8, it is characterised in that the brown sugar aqueous solution Concentration is 100~200mg/L.
- 10. the method for preventing stichopus japonicus from producing stress reaction according to claim 8 or claim 9, it is characterised in that the stichopus japonicus recovery Do not fed in 10~14h afterwards, feed feed bait according to the 0.5~2% of stichopus japonicus weight first, it is extensive again after 24~36h after recovery Multiple normal feeding volume.
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