CN107988296A - A kind of preparation method of cotton dregs peptide - Google Patents
A kind of preparation method of cotton dregs peptide Download PDFInfo
- Publication number
- CN107988296A CN107988296A CN201710986352.2A CN201710986352A CN107988296A CN 107988296 A CN107988296 A CN 107988296A CN 201710986352 A CN201710986352 A CN 201710986352A CN 107988296 A CN107988296 A CN 107988296A
- Authority
- CN
- China
- Prior art keywords
- cotton dregs
- fermentation
- product
- peptide
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of preparation method of cotton dregs peptide, it is the raw material pulvis after crushing cotton dregs or cottonseed, add water to be inoculated with zymophyte after mixing and carry out solid fermentation, solid fermentation product adds water and compound protease carries out liquid enzymolysis, secondary fermentation is finally carried out with anaerobic bacteria again, cotton dregs peptide finished product is obtained after obtaining the drying of secondary fermentation product.Beneficial effects of the present invention are：A kind of preparation method of cotton dregs peptide provided by the invention, the advantages of combining solid fermentation method and liquid enzymatic isolation method, overcome the deficiency of the two, raw material is used as using cotton dregs and/or cotton dregs powder, reduce cost of material, a large amount of amounts for reducing external enzyme, obtain higher percent hydrolysis, used as feed addictive, absorbing proteins rate is added, improves lean meat percentage and poultry disease resistance, and has effectively facilitated the growth rate of poultry, promotion and popularization application to cotton dregs peptide has great facilitation, while has made product guarantee to subtracting anti-, nonreactive popularization execution again.
Description
Technical field
The present invention relates to cotton dregs deep process technology field, and in particular to a kind of preparation method of cotton dregs peptide.
Background technology
Cotton dregs peptide is the deep processed product of cotton dregs, and mainly using the method for biology, transformation cottonseed meal matter molecule is low
The peptide of molecule, makes its physiology and trophic function significantly improve and improve.Compared with traditional protein, the production technology of cotton dregs peptide
It is more complicated, cost price is higher, but it absorbs with easy to digest, can be rapidly provided human body energy, without albuminous degeneration, decomposes cotton
Phenol, the small and heated characteristic such as do not solidify of no residue, liquid viscosity.
The microorganism type of inoculation is different and variant according to data source, have Mucor, aspergillus oryzae, aspergillus niger, bacillus natto,
Bacillus subtilis etc., is usually only inoculated with a kind of microorganism therein and ferments.Ferment after a certain period of time, then by separating,
Concentration and drying, are made cotton dregs peptide product.Liquid fermentation method production cotton dregs peptide is of high cost, and product quality has fluctuation.Mainly
There are the problem of following several respects：
1st, the condition of high-temperature sterilization and sealing is needed, investment is big.
2nd, the condition of the growth conditions of thalline and enzymolysis requires variant, cannot meet both requirements in production at the same time.
Because in fermentation process, bacterium produces enzyme, enzyme decomposing protein substantially plays a role at the same time.Digesting desired temperature usually will height
In the growth temperature requirement of bacterium.Meet enzymatic hydrolysis condition, the growth of bacterium will certainly be influenced, be also such in turn.Enzyme is derived from
Bacterium, therefore can only be controlled on producing by the growth temperature of bacterium.Therefore, production efficiency is affected, and product yield is low.
3rd, ferment first producing strains, then produces enzyme, then cottonseed meal is hydrolyzed by the effect of enzyme, due to the enzyme to generation
Amount and enzymatic hydrolysis condition can't be controlled effectively, mutually tackled the hydrolysis degree of protein and can not accurately be controlled.Therefore, cotton dregs peptide
The stabilised quality of product is difficult to be protected.
Just because of these are insufficient, liquid fermentation method production cotton dregs peptide does not form industrialized production.
The method production cotton dregs polypeptide of liquid enzymolysis is also first to add water to mix in cotton dregs powder or cotton dregs protein isolate, Ran Houzhi
Connect the one or more compound proteases of addition and carry out insulation hydrolysis.When using composite protease hydrolysis, some researchers are to adopt
With the mode once added, some are by the way of secondary addition, during enzymolysis, are added in different time sections different
Enzyme.In the protease in the mould source of hydrolase type hairiness that uses, the acid protease of Aspergillus niger origin, aspergillus oryzae source
Property protease, the protease (Alcalase, Alcakase, Protamex etc.) of bacterial origin, the protease (pawpaw of plant origin
Protease, bromelain etc.).After the completion of hydrolysis, then heating enzyme deactivation, cooling, separation, concentration and drying are passed sequentially through, finally made
Into cotton dregs peptide product.And solid enzymatic hydrolysis method production cotton dregs peptide working condition is gentleer, it is not necessary to high-temperature high-pressure apparatus, with liquid
Fermentation method compares, and invests opposite reduce.Secondly, solid enzymatic hydrolysis method production cotton dregs peptide the amount and enzymatic hydrolysis condition of enzyme can be realized compared with
It is accurately controlled, product quality is more stable.Solid enzymatic hydrolysis method production cotton dregs peptide has been enter into industrialized production, but liquid enzymatic isolation method
Cotton dregs peptide still high expensive is produced, limits the application of cotton dregs peptide product.The reason for finished product is higher：First, liquid enzymatic isolation method pair
The low price raw materials such as cotton dregs powder hydrolysis yield is low, therefore using the raw material of high-purity high price, is generally made using cotton dregs protein isolate
For raw materials for production, equal protein matter cost of material adds more than 5 times compared with cotton dregs powder；Second, the enzyme amount that liquid enzymatic isolation method uses
Greatly, outsourcing enzyme is of high cost.
The content of the invention
The purpose of the present invention aiming at it is above-mentioned in the prior art the defects of, combine solid fermentation method and liquid enzymatic isolation method
The advantages of, overcome the deficiency of the two, there is provided a kind of to digest what is be combined using two-stage method, i.e. by solid fermentation and liquid
Method manufactures cotton dregs peptide.
To achieve these goals, technical solution provided by the invention is：A kind of preparation method of cotton dregs peptide, is by cotton dregs
And/or the raw material pulvis after cottonseed crushing, add water to be inoculated with zymophyte after mixing and carry out solid fermentation, solid fermentation product adds
Water and compound protease carry out liquid enzymolysis, finally carry out secondary fermentation again with anaerobic bacteria, after obtaining the drying of secondary fermentation product
Obtain cotton dregs peptide finished product.
Further, the preparation method of above-mentioned a kind of cotton dregs peptide, comprises the following steps：
1) crush：
Take that cotton dregs or cottonseed carry out coarse crushing successively and fine powder is broken, be crushed to more than or equal to after 80 mesh, obtain raw material pulvis；
2) solid fermentation：
The raw material pulvis that step 1) is obtained, after addition water is mixed evenly, inoculation fermentation bacterium, windrow carries out temperature control and consolidates
Body ferments, and obtains solid fermentation product；The additional proportion of raw material pulvis and water is calculated as 1 according to mass ratio：(0.5-1.5), fermentation
Bacterium is bacillus subtilis, and inoculative proportion is calculated as 0.1-5% according to mass percent, and fermentation temperature is 25-40 DEG C, feed height
For 50-90cm, fermentation time is 1.5-3 days；
3) liquid digests：
Step 2) is obtained after solid fermentation product mixes with water, adding compound protease, carrying out heat insulation liquid enzymolysis, obtain
To enzymolysis product；The additional proportion of solid fermentation product and water is when 1 according to quality：(7-10), the addition of compound protease
Amount is calculated as the 0.05-0.15% of solid fermentation product and water gross mass according to mass percent, and hydrolysis temperature is 45-60 DEG C, enzyme
When the solution time is 1.5-13 small；
4) anaerobism secondary fermentation：
The enzymolysis product that step 3) is obtained is placed in the breathing bag equipped with one-way cock, is added anaerobic bacteria and is carried out secondary hair
Ferment, obtains secondary fermentation product；Anaerobic bacteria is lactic acid bacteria, and anaerobic bacteria inoculative proportion is calculated as 1.2-1.5% according to mass percent,
Anaerobic fermentation temperature is 20-28 DEG C, and the anaerobic fermentation time is 3-5 days；
5) it is dry：
The secondary fermentation product that step 4) obtains is dried, drying is 7.5-8.5% to moisture, that is, obtains cotton
Dregs of rice peptide product.
Further, the preparation method of above-mentioned a kind of cotton dregs peptide, in the step 1), coarse crushing be using pulverizer into
Row crushes, and broken fine powder is crushed using pulverizing mill, is crushed to 100 mesh.
Further, the preparation method of above-mentioned a kind of cotton dregs peptide, in the step 2), the addition ratio of raw material pulvis and water
Example is calculated as 1 according to mass ratio：(0.8-1.2), zymophyte are bacillus subtilis, and inoculative proportion is calculated as 4- according to mass percent
5%, fermentation temperature is 28-40 DEG C, feed height 60-80cm, and fermentation time is 1.5-2.5 days.
Further, the preparation method of above-mentioned a kind of cotton dregs peptide, in the step 2), the addition ratio of raw material pulvis and water
Example is calculated as 1 according to mass ratio：1, zymophyte is bacillus subtilis, and inoculative proportion is calculated as 5% according to mass percent, fermentation temperature
Spend for 34 DEG C, feed height 50cm, fermentation time is 2 days.
Further, the preparation method of above-mentioned a kind of cotton dregs peptide, in the step 3), solid fermentation product and water plus
It is when 1 to enter ratio according to quality：(8.5-9.5), the addition of compound protease are calculated as solid fermentation according to mass percent
The 0.08-0.1% of product and water gross mass, hydrolysis temperature are 50-55 DEG C, when enzymolysis time is 2-10 small.
Further, the preparation method of above-mentioned a kind of cotton dregs peptide, in the step 3), solid fermentation product and water plus
It is when 1 to enter ratio according to quality：9, the addition of compound protease is calculated as solid fermentation product and water according to mass percent
The 0.09% of gross mass, hydrolysis temperature are 52 DEG C, when enzymolysis time is 6 small.
Further, the preparation method of above-mentioned a kind of cotton dregs peptide, in the step 4), anaerobic bacteria is lactic acid bacteria, anaerobism
Bacterium inoculative proportion is calculated as 1.3-1.4% according to mass percent, and anaerobic fermentation temperature is 22-26 DEG C, and the anaerobic fermentation time is
3.5-4.5 my god.
Further, the preparation method of above-mentioned a kind of cotton dregs peptide, in the step 4), anaerobic bacteria is lactic acid bacteria, anaerobism
Bacterium inoculative proportion is calculated as 1.35% according to mass percent, and anaerobic fermentation temperature is 24 DEG C, and the anaerobic fermentation time is 4 days.
Further, the preparation method of above-mentioned a kind of cotton dregs peptide, in the step 5), drying mode is done using low temperature
Dry, moisture is controlled 8%.
Beneficial effects of the present invention are：A kind of preparation method of cotton dregs peptide provided by the invention, combines solid fermentation method
The advantages of with liquid enzymatic isolation method, the deficiency of the two is overcome, using cotton dregs and/or cotton dregs powder as raw material, reduce cost of material, greatly
Amount reduces the amount of external enzyme, obtains higher percent hydrolysis, is used as feed addictive, add absorbing proteins rate, improve
Lean meat percentage and poultry disease resistance, and the growth rate of poultry has been effectively facilitated, the promotion and popularization application to cotton dregs peptide has emphatically
Big facilitation, while again product guarantee has been made to subtracting anti-, nonreactive popularization execution.
Embodiment
Invention formulation and microbial inoculum source are as shown in table 1.
Table 1
| Bacillus subtilis | Lactic acid bacteria | Enzyme preparation | Saccharomycete |
| Shandong Bora Li Lai biotech firms | Shandong Bora Li Lai biotech firms | Biological Co., Ltd in the benefit of Wuhan | Angel Yeast joint-stock company |
| Shandong, which is raised, adds word (2015) 926005 | Shandong, which is raised, adds word (2015) 906005 | Hubei Province, which is raised, adds word (2016) 025017 | SC1314514020019 |
Embodiment 1：
A kind of preparation method of cotton dregs peptide, is the raw material pulvis after crushing cotton dregs and cottonseed, adds water to be inoculated with hair after mixing
Yeast-like fungi carries out solid fermentation, and solid fermentation product adds water and compound protease carries out liquid enzymolysis, finally again with anaerobic bacteria
Secondary fermentation is carried out, cotton dregs peptide finished product is obtained after obtaining the drying of secondary fermentation product, specifically includes following steps：
1) crush：
Take that cotton dregs or cottonseed carry out coarse crushing successively and fine powder is broken, after being crushed to 80 mesh, obtain raw material pulvis；
2) solid fermentation：
The raw material pulvis that step 1) is obtained, after addition water is mixed evenly, inoculation fermentation bacterium, windrow carries out temperature control and consolidates
Body ferments, and obtains solid fermentation product；The additional proportion of raw material pulvis and water is calculated as 1 according to mass ratio：0.5, zymophyte is withered
Careless bacillus, inoculative proportion are calculated as 0.1% according to mass percent, and fermentation temperature is 25 DEG C, feed height 50cm, fermentation
Time is
1.5 my god；
3) liquid digests：
Step 2) is obtained after solid fermentation product mixes with water, adding compound protease, carrying out heat insulation liquid enzymolysis, obtain
To enzymolysis product；The additional proportion of solid fermentation product and water is when 1 according to quality：7, the addition of compound protease according to
Mass percent is calculated as the 0.05% of solid fermentation product and water gross mass, and hydrolysis temperature is 45 DEG C, and enzymolysis time is small for 1.5
When；
4) anaerobism secondary fermentation：
The enzymolysis product that step 3) is obtained is placed in the breathing bag equipped with one-way cock, is added anaerobic bacteria and is carried out secondary hair
Ferment, obtains secondary fermentation product；Anaerobic bacteria is lactic acid bacteria, and anaerobic bacteria inoculative proportion is calculated as 1.2% according to mass percent, anaerobism
Fermentation temperature is 20 DEG C, and the anaerobic fermentation time is 3 days；
5) it is dry：
The secondary fermentation product that step 4) obtains is dried, drying mode uses low temperature drying, and drying to moisture contains
Measure as 7.5%, that is, obtain cotton dregs peptide product.
Embodiment 2：
A kind of preparation method of cotton dregs peptide, is the raw material pulvis after cotton dregs are crushed, add water mix after be inoculated with zymophyte into
Row solid fermentation, solid fermentation product adds water and compound protease carries out liquid enzymolysis, finally carries out two again with anaerobic bacteria
Secondary fermentation, obtains cotton dregs peptide finished product after obtaining the drying of secondary fermentation product, specifically includes following steps：
1) crush：
Take that cotton dregs or cottonseed carry out coarse crushing successively and fine powder is broken, after being crushed to 120 mesh, obtain raw material pulvis；
2) solid fermentation：
The raw material pulvis that step 1) is obtained, after addition water is mixed evenly, inoculation fermentation bacterium, windrow carries out temperature control and consolidates
Body ferments, and obtains solid fermentation product；The additional proportion of raw material pulvis and water is calculated as 1 according to mass ratio：1.5, zymophyte is withered
Careless bacillus, inoculative proportion are calculated as 5% according to mass percent, and fermentation temperature is 40 DEG C, feed height 90cm, during fermentation
Between be 3 days；
3) liquid digests：
Step 2) is obtained after solid fermentation product mixes with water, adding compound protease, carrying out heat insulation liquid enzymolysis, obtain
To enzymolysis product；The additional proportion of solid fermentation product and water is when 1 according to quality：10, the addition of compound protease is pressed
The 0.15% of solid fermentation product and water gross mass is calculated as according to mass percent, hydrolysis temperature is 60 DEG C, and enzymolysis time is small for 13
When；
4) anaerobism secondary fermentation：
The enzymolysis product that step 3) is obtained is placed in the breathing bag equipped with one-way cock, is added anaerobic bacteria and is carried out secondary hair
Ferment, obtains secondary fermentation product；Anaerobic bacteria is lactic acid bacteria, and anaerobic bacteria inoculative proportion is calculated as 1.5% according to mass percent, anaerobism
Fermentation temperature is 28 DEG C, and the anaerobic fermentation time is 5 days；
5) it is dry：
The secondary fermentation product that step 4) obtains is dried, drying mode uses low temperature drying, and drying to moisture contains
Measure as 8.5%, that is, obtain cotton dregs peptide product.
Embodiment 3：
A kind of preparation method of cotton dregs peptide, is the raw material pulvis after cottonseed is crushed, add water mix after be inoculated with zymophyte into
Row solid fermentation, solid fermentation product adds water and compound protease carries out liquid enzymolysis, finally carries out two again with anaerobic bacteria
Secondary fermentation, obtains cotton dregs peptide finished product after obtaining the drying of secondary fermentation product, specifically includes following steps：
1) crush：
Take that cotton dregs or cottonseed carry out coarse crushing successively and fine powder is broken, after being crushed to 90 mesh, obtain raw material pulvis；
2) solid fermentation：
The raw material pulvis that step 1) is obtained, after addition water is mixed evenly, inoculation fermentation bacterium, windrow carries out temperature control and consolidates
Body ferments, and obtains solid fermentation product；The additional proportion of raw material pulvis and water is calculated as 1 according to mass ratio：0.8, zymophyte is withered
Careless bacillus, inoculative proportion are calculated as 4% according to mass percent, and fermentation temperature is 28 DEG C, feed height 60cm, during fermentation
Between be 1.5 days；
3) liquid digests：
Step 2) is obtained after solid fermentation product mixes with water, adding compound protease, carrying out heat insulation liquid enzymolysis, obtain
To enzymolysis product；The additional proportion of solid fermentation product and water is when 1 according to quality：8.5, the addition of compound protease is pressed
The 0.08% of solid fermentation product and water gross mass is calculated as according to mass percent, hydrolysis temperature is 50 DEG C, and enzymolysis time is small for 2
When；
4) anaerobism secondary fermentation：
The enzymolysis product that step 3) is obtained is placed in the breathing bag equipped with one-way cock, is added anaerobic bacteria and is carried out secondary hair
Ferment, obtains secondary fermentation product；Anaerobic bacteria is lactic acid bacteria, and anaerobic bacteria inoculative proportion is calculated as 1.3% according to mass percent, anaerobism
Fermentation temperature is 22 DEG C, and the anaerobic fermentation time is 3.5 days；
5) it is dry：
The secondary fermentation product that step 4) obtains is dried, drying mode uses low temperature drying, and drying to moisture contains
Measure as 7.5%, that is, obtain cotton dregs peptide product.
Embodiment 4：
A kind of preparation method of cotton dregs peptide, is the raw material pulvis after cotton dregs are crushed, add water mix after be inoculated with zymophyte into
Row solid fermentation, solid fermentation product adds water and compound protease carries out liquid enzymolysis, finally carries out two again with anaerobic bacteria
Secondary fermentation, obtains cotton dregs peptide finished product after obtaining the drying of secondary fermentation product, specifically includes following steps：
1) crush：
Take that cotton dregs or cottonseed carry out coarse crushing successively and fine powder is broken, after being crushed to 110 mesh, obtain raw material pulvis；
2) solid fermentation：
The raw material pulvis that step 1) is obtained, after addition water is mixed evenly, inoculation fermentation bacterium, windrow carries out temperature control and consolidates
Body ferments, and obtains solid fermentation product；The additional proportion of raw material pulvis and water is calculated as 1 according to mass ratio：1.2, zymophyte is withered
Careless bacillus, inoculative proportion are calculated as 5% according to mass percent, and fermentation temperature is 40 DEG C, feed height 80cm, during fermentation
Between be 2.5 days；
3) liquid digests：
Step 2) is obtained after solid fermentation product mixes with water, adding compound protease, carrying out heat insulation liquid enzymolysis, obtain
To enzymolysis product；The additional proportion of solid fermentation product and water is when 1 according to quality：9.5, the addition of compound protease is pressed
The 0.1% of solid fermentation product and water gross mass is calculated as according to mass percent, hydrolysis temperature is 55 DEG C, and enzymolysis time is small for 10
When；
4) anaerobism secondary fermentation：
The enzymolysis product that step 3) is obtained is placed in the breathing bag equipped with one-way cock, is added anaerobic bacteria and is carried out secondary hair
Ferment, obtains secondary fermentation product；Anaerobic bacteria is lactic acid bacteria, and anaerobic bacteria inoculative proportion is calculated as 1.4% according to mass percent, anaerobism
Fermentation temperature is 26 DEG C, and the anaerobic fermentation time is 4.5 days；
5) it is dry：
The secondary fermentation product that step 4) obtains is dried, drying mode uses low temperature drying, and drying to moisture contains
Measure as 8.5%, that is, obtain cotton dregs peptide product.
Embodiment 5：
A kind of preparation method of cotton dregs peptide, is the raw material pulvis after crushing cotton dregs and cottonseed, adds water to be inoculated with hair after mixing
Yeast-like fungi carries out solid fermentation, and solid fermentation product adds water and compound protease carries out liquid enzymolysis, finally again with anaerobic bacteria
Secondary fermentation is carried out, cotton dregs peptide finished product is obtained after obtaining the drying of secondary fermentation product, specifically includes following steps：
1) crush：
Take that cotton dregs or cottonseed carry out coarse crushing successively and fine powder is broken, after being crushed to 110 mesh, obtain raw material pulvis；
2) solid fermentation：
The raw material pulvis that step 1) is obtained, after addition water is mixed evenly, inoculation fermentation bacterium, windrow carries out temperature control and consolidates
Body ferments, and obtains solid fermentation product；The additional proportion of raw material pulvis and water is calculated as 1 according to mass ratio：1, zymophyte is withered grass
Bacillus, inoculative proportion are calculated as 5% according to mass percent, and fermentation temperature is 34 DEG C, feed height 50cm, fermentation time
For 2 days；
3) liquid digests：
Step 2) is obtained after solid fermentation product mixes with water, adding compound protease, carrying out heat insulation liquid enzymolysis, obtain
To enzymolysis product；The additional proportion of solid fermentation product and water is when 1 according to quality：9, the addition of compound protease according to
Mass percent is calculated as the 0.09% of solid fermentation product and water gross mass, and hydrolysis temperature is 52 DEG C, when enzymolysis time is 6 small；
4) anaerobism secondary fermentation：
The enzymolysis product that step 3) is obtained is placed in the breathing bag equipped with one-way cock, is added anaerobic bacteria and is carried out secondary hair
Ferment, obtains secondary fermentation product；Anaerobic bacteria is lactic acid bacteria, and anaerobic bacteria inoculative proportion is calculated as 1.35% according to mass percent, detests
Aerobe fermentation temperature is 24 DEG C, and the anaerobic fermentation time is 4 days；
5) it is dry：
The secondary fermentation product that step 4) obtains is dried, drying mode uses low temperature drying, and drying to moisture contains
Measure as 8%, that is, obtain cotton dregs peptide product.
Embodiment 6：
Manufacturing process contrast is as shown in table 2：
Table 2
| Mode | Process | Put thing | Peptide albumen generation rate | Cost | |
| Solid fermentation | Strain adds | Mixing, addition, low temperature, drying | Fermentation vat | Higher than liquid state fermentation | Less than liquid state fermentation 50% |
| Liquid digests | Protease liquefies | Cooling, separation, concentration, drying | Rustless steel container |
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still may be used
To modify to the technical solution described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic.
Within the spirit and principles of the invention, any modification, equivalent replacement, improvement and so on, should be included in the present invention's
Within protection domain.
Claims (10)
1. a kind of preparation method of cotton dregs peptide, it is characterised in that be the raw material pulvis after crushing cotton dregs and/or cottonseed, add water
Zymophyte is inoculated with after mixing and carries out solid fermentation, solid fermentation product adds water and compound protease carries out liquid enzymolysis, most
Secondary fermentation is carried out with anaerobic bacteria again afterwards, cotton dregs peptide finished product is obtained after obtaining the drying of secondary fermentation product.
2. the preparation method of a kind of cotton dregs peptide according to claim 1, it is characterised in that comprise the following steps：
1) crush：
Take that cotton dregs or cottonseed carry out coarse crushing successively and fine powder is broken, be crushed to more than or equal to after 80 mesh, obtain raw material pulvis；
2) solid fermentation：
The raw material pulvis that step 1) is obtained, after addition water is mixed evenly, inoculation fermentation bacterium, windrow carries out temperature control solid hair
Ferment, obtains solid fermentation product；The additional proportion of raw material pulvis and water is calculated as 1 according to mass ratio：(0.5-1.5), zymophyte are
Bacillus subtilis, inoculative proportion are calculated as 0.1-5% according to mass percent, and fermentation temperature is 25-40 DEG C, and feed height is
50-90cm, fermentation time are 1.5-3 days；
3) liquid digests：
Step 2) is obtained after solid fermentation product mixes with water, adding compound protease, carrying out heat insulation liquid enzymolysis, obtain enzyme
Solve product；The additional proportion of solid fermentation product and water is when 1 according to quality：(7-10), the addition of compound protease are pressed
It is calculated as the 0.05-0.15% of solid fermentation product and water gross mass according to mass percent, hydrolysis temperature is 45-60 DEG C, during enzymolysis
Between for 1.5-13 it is small when；
4) anaerobism secondary fermentation：
The enzymolysis product that step 3) is obtained is placed in the breathing bag equipped with one-way cock, is added anaerobic bacteria and is carried out secondary fermentation,
Obtain secondary fermentation product；Anaerobic bacteria is lactic acid bacteria, and anaerobic bacteria inoculative proportion is calculated as 1.2-1.5% according to mass percent, detests
Aerobe fermentation temperature is 20-28 DEG C, and the anaerobic fermentation time is 3-5 days；
5) it is dry：
The secondary fermentation product that step 4) obtains is dried, drying is 7.5-8.5% to moisture, that is, obtains cotton dregs peptide
Product.
A kind of 3. preparation method of cotton dregs peptide according to claim 2, it is characterised in that in the step 1), coarse crushing
It is to be crushed using pulverizer, broken fine powder is crushed using pulverizing mill, is crushed to 100 mesh.
A kind of 4. preparation method of cotton dregs peptide according to claim 2, it is characterised in that in the step 2), raw material powder
The additional proportion of agent and water is calculated as 1 according to mass ratio：(0.8-1.2), zymophyte are bacillus subtilis, and inoculative proportion is according to matter
Amount percentages are 4-5%, and fermentation temperature is 28-40 DEG C, feed height 60-80cm, and fermentation time is 1.5-2.5 days.
A kind of 5. preparation method of cotton dregs peptide according to claim 2, it is characterised in that in the step 2), raw material powder
The additional proportion of agent and water is calculated as 1 according to mass ratio：1, zymophyte is bacillus subtilis, and inoculative proportion is according to mass percent
5% is calculated as, fermentation temperature is 34 DEG C, feed height 50cm, and fermentation time is 2 days.
A kind of 6. preparation method of cotton dregs peptide according to claim 2, it is characterised in that in the step 3), solid hair
The additional proportion of ferment product and water is when 1 according to quality：(8.5-9.5), the addition of compound protease is according to quality percentage
Than the 0.08-0.1% for being calculated as solid fermentation product and water gross mass, hydrolysis temperature is 50-55 DEG C, and enzymolysis time is small for 2-10
When.
A kind of 7. preparation method of cotton dregs peptide according to claim 2, it is characterised in that in the step 3), solid hair
The additional proportion of ferment product and water is when 1 according to quality：9, the addition of compound protease is calculated as admittedly according to mass percent
The 0.09% of body tunning and water gross mass, hydrolysis temperature are 52 DEG C, when enzymolysis time is 6 small.
A kind of 8. preparation method of cotton dregs peptide according to claim 2, it is characterised in that in the step 4), anaerobic bacteria
For lactic acid bacteria, anaerobic bacteria inoculative proportion is calculated as 1.3-1.4% according to mass percent, and anaerobic fermentation temperature is 22-26 DEG C, anaerobism
Fermentation time is 3.5-4.5 days.
A kind of 9. preparation method of cotton dregs peptide according to claim 2, it is characterised in that in the step 4), anaerobic bacteria
For lactic acid bacteria, anaerobic bacteria inoculative proportion is calculated as 1.35% according to mass percent, and anaerobic fermentation temperature is 24 DEG C, during anaerobic fermentation
Between be 4 days.
A kind of 10. preparation method of cotton dregs peptide according to claim 2, it is characterised in that in the step 5), drying side
Formula uses low temperature drying, and moisture is controlled 8%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710986352.2A CN107988296A (en) | 2017-10-20 | 2017-10-20 | A kind of preparation method of cotton dregs peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710986352.2A CN107988296A (en) | 2017-10-20 | 2017-10-20 | A kind of preparation method of cotton dregs peptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107988296A true CN107988296A (en) | 2018-05-04 |
Family
ID=62029897
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710986352.2A Pending CN107988296A (en) | 2017-10-20 | 2017-10-20 | A kind of preparation method of cotton dregs peptide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107988296A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109007266A (en) * | 2018-08-13 | 2018-12-18 | 浙江大学 | A kind of method that tealeaf residue solid state fermentation prepares feedstuff |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720853A (en) * | 2009-10-19 | 2010-06-09 | 李军训 | Technology for fermenting and decomposing protein feed with protease in two steps by using probiotics |
| CN102742719A (en) * | 2011-04-19 | 2012-10-24 | 上海新农饲料有限公司 | Preparation method of probiotic solid-state fermented complete feed |
| CN104186957A (en) * | 2014-10-01 | 2014-12-10 | 青岛嘉瑞生物技术有限公司 | Method for improving forage nutritional value of cottonseed meal through microbial fermentation |
| CN104304805A (en) * | 2014-11-05 | 2015-01-28 | 盐城工学院 | Efficient environment-friendly compound feed for perineries aibuhitensis |
| CN106173189A (en) * | 2015-05-08 | 2016-12-07 | 上海邦成生物工程有限公司 | A kind of method of solid fermentation cottonseed meal |
-
2017
- 2017-10-20 CN CN201710986352.2A patent/CN107988296A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720853A (en) * | 2009-10-19 | 2010-06-09 | 李军训 | Technology for fermenting and decomposing protein feed with protease in two steps by using probiotics |
| CN102742719A (en) * | 2011-04-19 | 2012-10-24 | 上海新农饲料有限公司 | Preparation method of probiotic solid-state fermented complete feed |
| CN104186957A (en) * | 2014-10-01 | 2014-12-10 | 青岛嘉瑞生物技术有限公司 | Method for improving forage nutritional value of cottonseed meal through microbial fermentation |
| CN104304805A (en) * | 2014-11-05 | 2015-01-28 | 盐城工学院 | Efficient environment-friendly compound feed for perineries aibuhitensis |
| CN106173189A (en) * | 2015-05-08 | 2016-12-07 | 上海邦成生物工程有限公司 | A kind of method of solid fermentation cottonseed meal |
Non-Patent Citations (1)
| Title |
|---|
| ZHANG WEN-JU等: "Effect of selected fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal", 《J ZHEJIANG UNIV SCI B》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109007266A (en) * | 2018-08-13 | 2018-12-18 | 浙江大学 | A kind of method that tealeaf residue solid state fermentation prepares feedstuff |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100392092C (en) | Soybean peptide production method | |
| CN104012755B (en) | A kind of method of two-step fermentation High-efficient Production fermented bean dregs | |
| CN102994605B (en) | Method for producing high-efficiency biological peptide through enzymolysis and microbe fermentation | |
| CN104171759A (en) | Edible mushroom residue feed preparation process | |
| CN102972635A (en) | Method for producing microorganism feed additive by utilizing probiotics and mixed meal | |
| CN105884405A (en) | Preparation method of amino acid-polypeptide liquid fertilizer | |
| CN106173190A (en) | A kind of preparation method of soybean polypeptide albumen feedstuff | |
| CN105581240A (en) | Preparation method of rice bran fermented whole flour | |
| CN106538823A (en) | A kind of production technology of highly acidity fermented bean cake | |
| CN103478417A (en) | Method for producing fermented soybean meal by two-step inoculation segmented solid-state fermentation | |
| CN104054903A (en) | Production process of fermented cottonseed meal | |
| CN101218338B (en) | Method for producing liquid koji | |
| CN107156808A (en) | A kind of preparation method of matrimony vine ferment | |
| CN106417900A (en) | Processing method and application of bean pulp for feed | |
| CN104509683A (en) | Method for preparing yellow corn silage feed by compounding and fermenting shells of bamboo shoots | |
| CN111296723A (en) | Probiotic agent for multi-strain combined solid state fermentation of rapeseed meal and fermentation method thereof | |
| CN103805660B (en) | A kind of method utilizing cheap raw material to produce nisin | |
| CN103642885A (en) | Method for producing wheat biological active peptide by fermentation | |
| CN109198185A (en) | A kind of method that bean dregs solid state fermentation prepares culture of microorganism | |
| CN107988296A (en) | A kind of preparation method of cotton dregs peptide | |
| CN102392008B (en) | Bioprotein capable of replacing protein raw material and preparation method of bioprotein | |
| CN102389024A (en) | Method for producing feeding peanut peptide by anaerobic fermentation of peanut meal | |
| CN105861391A (en) | Normal temperature compound microbial fermentation agent | |
| CN101491289A (en) | Rape seed protein feedstuff and preparation method thereof | |
| CN106085917A (en) | A kind of bacillus subtilis expanding propagation culture medium and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20180426 Address after: No. 87, enterprise street, Wen Zhen, Wenchang City, Hainan Province, Hainan Applicant after: Hainan Hui biotech Co., Ltd. Address before: 570125 D, 19 / F, business consulate 15, Jinmao West Road, Longhua District, Haikou, Hainan. Applicant before: Hainan coner diet Biotechnology Co., Ltd. |
|
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180504 |