CN107976438A - A kind of rapid assay methods of Pb in Cosmetics - Google Patents
A kind of rapid assay methods of Pb in Cosmetics Download PDFInfo
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- CN107976438A CN107976438A CN201711188679.1A CN201711188679A CN107976438A CN 107976438 A CN107976438 A CN 107976438A CN 201711188679 A CN201711188679 A CN 201711188679A CN 107976438 A CN107976438 A CN 107976438A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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Abstract
The present invention relates to detection technique field, more particularly to a kind of rapid assay methods of Pb in Cosmetics.By sample to be tested after calcination, addition acetum carries out dissolving and is configured to prepare liquid；Prepare liquid and lead standard solution are adsorbed respectively using two test strips of same size, and two test strips develop the color to uniform sprinkling saturated hydrogen sulphide solution respectively, and carry out colorimetric.The present invention is easy to operate, and agents useful for same is readily available, and is adapted to daily quick detection to use, testing result is directly perceived, and detection time is short.
Description
（One）Technical field
The present invention relates to detection technique field, more particularly to a kind of rapid assay methods of Pb in Cosmetics.
（Two）Background technology
Lead is human body uniquely unwanted trace element, it is that nondegradable pollutant U.S.A of stabilization a kind of is a kind of serious danger
The heavy metal element of evil human health.Lead can hinder the formation of haemocyte, cause the intelligence of people to decline, and study, job performance are low
Fall；It can make one the slow poisoning symptom such as phrenoblabia, bad dream, insomnia, headache occur to certain Chengdu；Severe patient there may also be
Weak, loss of appetite, nausea, abdominal distension, abdominal pain etc., the more seriously growth of its vivid infant, intellectual development, spiritual row
For with the brain function such as learning and memory, or even dementia can be caused.
Lead has human skin preferable whitening, freckle effect, and is easily absorbed by the body, therefore much has whitening
The cosmetics of effect all contain certain lead composition.In addition the compound of money can make color more lasting, so wide by manufacturer
It is general to be applied in the cosmetics such as lipstick, nail polish, eye shadow, muffin, hair dye.
Heavy Metals in Cosmetics is exceeded very big to body effect,《Cosmetics health specification》（Version in 2007）To cosmetics
Middle lead content has extremely stringent regulation, its content must not exceed 40ppm respectively.But country and professional standard are to a huge sum of money at present
The detection of category is concentrated mainly on high-end the precise instrument context of detection, sieves soon soon examine the scarcity of technological means, and supervision is mainly
Subsequent supervision, causes to produce little effect.
（Three）The content of the invention
In order to compensate for the shortcomings of the prior art, the present invention provides a kind of rapid assay methods of Pb in Cosmetics.
The present invention is achieved through the following technical solutions：
A kind of rapid assay methods of Pb in Cosmetics, it is characterised in that：By sample to be tested after calcination, acetum is added
Carry out dissolving and be configured to prepare liquid；Prepare liquid and lead standard solution are adsorbed respectively using two test strips of same size, point
Other two test strips develop the color to uniform sprinkling saturated hydrogen sulphide solution, and carry out colorimetric.
Wherein, the acetum volume fraction is 50%.
Wherein, the lead standard solution concentration is：10μg/ml~80μg/ml.
Including step in detail below：
（1）Prepare lead standard reserving solution：A certain amount of high pure nitric acid lead is weighed, is dissolved in the acetum that volume fraction is 1%,
Quantitatively move into volumetric flask, constant volume is diluted with water, lead standard reserving solution concentration, which is made, is：1mg/ml；
（2）Prepare lead standard solution：Draw lead standard reserving solution and be diluted to concentration as the μ g/ml of 10 μ g/ml ~ 80；
（3）Sample calcination：Take a certain amount of sample to add in crucible, carry out calcination untill not emitting white cigarette；
（4）Configure prepare liquid：Component after sample calcination is added in acetum and is dissolved, slightly boiling is heated to and keeps
5min, carries out dissolving constant volume after room temperature with purified water after cooling, filters insoluble impurity and obtains prepare liquid；
（5）Dried up after test strips are fully absorbed prepare liquid, saturated hydrogen sulphide solution is uniformly sprayed after paving；Take same test paper
Bar dries up after fully absorbing lead standard solution, and saturated hydrogen sulphide solution is uniformly sprayed after paving；Two test strips are compared
Color.
The beneficial effects of the invention are as follows：The present invention is easy to operate, and agents useful for same is readily available, and is adapted to daily quick detection
Use, testing result is directly perceived, and detection time is short.
（Four）Embodiment
Embodiment 1
（1）Prepare lead standard reserving solution（1mg/mL）:0.1598g high pure nitric acids lead [Pb (NO3) 2] is weighed, is dissolved in 10mL acetic acid
Solution（1%）In, quantitatively move into 100mL volumetric flasks, be diluted with water to scale.
（2）Prepare lead standard solution（10μg/mL）：Draw lead standard reserving solution（1mg/mL）1.0mL is in 100mL capacity
In bottle, scale is added water to.
（3）Lead standard solution develops the color：The test strips cut are soaked in 10min in standard solution, are taken out with blowing
Wind turbine dries up, and then uniformly sprinkling saturated hydrogen sulphide solution, wait develop the color.
（4）Sample treatment
About 5g samples are taken to carry out calcination on electric hot plate or stove in crucible, untill not emitting white cigarette, then add
10ml（1:1）Glacial acetic acid carry out sample dissolving, be heated to slightly boiling and keep 5min, carried out after being cooled to room temperature with purified water
Dissolving is settled to 10ml, filters insoluble impurity, the test strips cut is immersed in filtrate, to guarantee uniform pickup, this is molten
Liquid, clamps test strips with plastic tweezer and is absorbed repeatedly, after immersing 10min, takes out test strips, is dried up with hair-dryer, paved
Saturated hydrogen sulphide solution is uniformly sprayed afterwards；Compared with test strips colour developing is developed the color with lead standard solution, if color is more molten than lead standard
Liquid is shallow, then lead content is less than 5mg/kg in sample.
Embodiment 2
（1）Prepare lead standard reserving solution（1mg/mL）:0.1598g high pure nitric acids lead [Pb (NO3) 2] is weighed, is dissolved in 10mL acetic acid
Solution（1%）In, quantitatively move into 100mL volumetric flasks, be diluted with water to scale.
（2）Prepare lead standard solution（20μg/mL）：Draw lead standard reserving solution（1mg/mL）2.0mL is in 100mL capacity
In bottle, scale is added water to.
（3）Lead standard solution develops the color：The test strips cut are soaked in 10min in standard solution, are taken out with blowing
Wind turbine dries up, and then uniformly sprinkling saturated hydrogen sulphide solution, wait develop the color.
（4）Sample treatment
About 10g samples are taken to carry out calcination on electric hot plate or stove in crucible, untill not emitting white cigarette, then add
10ml（1:1）Glacial acetic acid carry out sample dissolving, be heated to slightly boiling and keep 5min, carried out after being cooled to room temperature with purified water
Dissolving is settled to 10ml, filters insoluble impurity, the test strips cut is immersed in filtrate, to guarantee uniform pickup, this is molten
Liquid, clamps test strips with plastic tweezer and is absorbed repeatedly, after immersing 10min, takes out test strips, is dried up with hair-dryer, paved
Saturated hydrogen sulphide solution is uniformly sprayed afterwards；Compared with test strips colour developing is developed the color with lead standard solution, if color is more molten than lead standard
Liquid is shallow, then lead content is less than 10mg/kg in sample.
Embodiment 3
（1）Prepare lead standard reserving solution（1mg/mL）:0.1598g high pure nitric acids lead [Pb (NO3) 2] is weighed, is dissolved in 10mL acetic acid
Solution（1%）In, quantitatively move into 100mL volumetric flasks, be diluted with water to scale.
（2）Prepare lead standard solution（40μg/mL）：Draw lead standard reserving solution（1mg/mL）4.0mL is in 100mL capacity
In bottle, scale is added water to.
（3）Lead standard solution develops the color：The test strips cut are soaked in 10min in standard solution, are taken out with blowing
Wind turbine dries up, and then uniformly sprinkling saturated hydrogen sulphide solution, wait develop the color.
（4）Sample treatment
About 20g samples are taken to carry out calcination on electric hot plate or stove in crucible, untill not emitting white cigarette, then add
10ml（1:1）Glacial acetic acid carry out sample dissolving, be heated to slightly boiling and keep 5min, carried out after being cooled to room temperature with purified water
Dissolving is settled to 10ml, filters insoluble impurity, the test strips cut is immersed in filtrate, to guarantee uniform pickup, this is molten
Liquid, clamps test strips with plastic tweezer and is absorbed repeatedly, after immersing 10min, takes out test strips, is dried up with hair-dryer, paved
Saturated hydrogen sulphide solution is uniformly sprayed afterwards；Compared with test strips colour developing is developed the color with lead standard solution, if color is more molten than lead standard
Liquid is shallow, then lead content is less than 20mg/kg in sample.
Embodiment 4
（1）Prepare lead standard reserving solution（1mg/mL）:0.1598g high pure nitric acids lead [Pb (NO3) 2] is weighed, is dissolved in 10mL acetic acid
Solution（1%）In, quantitatively move into 100mL volumetric flasks, be diluted with water to scale.
（2）Prepare lead standard solution（60μg/mL）：Draw lead standard reserving solution（1mg/mL）6.0mL is in 100mL capacity
In bottle, scale is added water to.
（3）Lead standard solution develops the color：The test strips cut are soaked in 10min in standard solution, are taken out with blowing
Wind turbine dries up, and then uniformly sprinkling saturated hydrogen sulphide solution, wait develop the color.
（4）Sample treatment
About 30g samples are taken to carry out calcination on electric hot plate or stove in crucible, untill not emitting white cigarette, then add
10ml（1:1）Glacial acetic acid carry out sample dissolving, be heated to slightly boiling and keep 5min, carried out after being cooled to room temperature with purified water
Dissolving is settled to 10ml, filters insoluble impurity, the test strips cut is immersed in filtrate, to guarantee uniform pickup, this is molten
Liquid, clamps test strips with plastic tweezer and is absorbed repeatedly, after immersing 10min, takes out test strips, is dried up with hair-dryer, paved
Saturated hydrogen sulphide solution is uniformly sprayed afterwards；Compared with test strips colour developing is developed the color with lead standard solution, if color is more molten than lead standard
Liquid is shallow, then lead content is less than 30mg/kg in sample.
Embodiment 5
（1）Prepare lead standard reserving solution（1mg/mL）:0.1598g high pure nitric acids lead [Pb (NO3) 2] is weighed, is dissolved in 10mL acetic acid
Solution（1%）In, quantitatively move into 100mL volumetric flasks, be diluted with water to scale.
（2）Prepare lead standard solution（80μg/mL）：Draw lead standard reserving solution（1mg/mL）8.0mL is in 100mL capacity
In bottle, scale is added water to.
（3）Lead standard solution develops the color：The test strips cut are soaked in 10min in standard solution, are taken out with blowing
Wind turbine dries up, and then uniformly sprinkling saturated hydrogen sulphide solution, wait develop the color.
（4）Sample treatment
About 30g samples are taken to carry out calcination on electric hot plate or stove in crucible, untill not emitting white cigarette, then add
10ml（1:1）Glacial acetic acid carry out sample dissolving, be heated to slightly boiling and keep 5min, carried out after being cooled to room temperature with purified water
Dissolving is settled to 10ml, filters insoluble impurity, the test strips cut is immersed in filtrate, to guarantee uniform pickup, this is molten
Liquid, clamps test strips with plastic tweezer and is absorbed repeatedly, after immersing 10min, takes out test strips, is dried up with hair-dryer, paved
Saturated hydrogen sulphide solution is uniformly sprayed afterwards；Compared with test strips colour developing is developed the color with lead standard solution, if color is more molten than lead standard
Liquid is shallow, then lead content is less than 40mg/kg in sample.
The present invention is described by way of example above, but the invention is not restricted to above-mentioned specific embodiment, it is all to be based on
Any changes or modifications that the present invention is done belong to the scope of protection of present invention.
Claims (4)
- A kind of 1. rapid assay methods of Pb in Cosmetics, it is characterised in that：By sample to be tested after calcination, it is molten to add acetic acid Liquid carries out dissolving and is configured to prepare liquid；Prepare liquid and lead standard solution are adsorbed respectively using two test strips of same size, Two test strips develop the color to uniform sprinkling saturated hydrogen sulphide solution respectively, and carry out colorimetric.
- 2. the rapid assay methods of Pb in Cosmetics according to claim 1, it is characterised in that：The acetum volume Fraction is 50%.
- 3. the rapid assay methods of Pb in Cosmetics according to claim 1, it is characterised in that：The lead standard solution Concentration is：10μg/ml~80μg/ml.
- 4. the rapid assay methods of Pb in Cosmetics according to claim 1, it is characterised in that：Including walking in detail below Suddenly：（1）Prepare lead standard reserving solution：A certain amount of high pure nitric acid lead is weighed, is dissolved in the acetum that volume fraction is 1%, Quantitatively move into volumetric flask, constant volume is diluted with water, lead standard reserving solution concentration, which is made, is：1mg/ml；（2）Prepare lead standard solution：Draw lead standard reserving solution and be diluted to concentration as the μ g/ml of 10 μ g/ml ~ 80；（3）Sample calcination：Take a certain amount of sample to add in crucible, carry out calcination untill not emitting white cigarette；（4）Configure prepare liquid：Component after sample calcination is added in acetum and is dissolved, slightly boiling is heated to and keeps 5min, carries out dissolving constant volume after room temperature with purified water after cooling, filters insoluble impurity and obtains prepare liquid；（5）Dried up after test strips are fully absorbed prepare liquid, saturated hydrogen sulphide solution is uniformly sprayed after paving；Take same test paper Bar dries up after fully absorbing lead standard solution, and saturated hydrogen sulphide solution is uniformly sprayed after paving；Two test strips are compared Color.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711188679.1A CN107976438A (en) | 2017-11-24 | 2017-11-24 | A kind of rapid assay methods of Pb in Cosmetics |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711188679.1A CN107976438A (en) | 2017-11-24 | 2017-11-24 | A kind of rapid assay methods of Pb in Cosmetics |
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| Publication Number | Publication Date |
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| CN107976438A true CN107976438A (en) | 2018-05-01 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203758918U (en) * | 2014-04-15 | 2014-08-06 | 安徽新华学院 | Device for joint detection on three heavy metal ions in cosmetics |
| CN103983642A (en) * | 2014-06-03 | 2014-08-13 | 三棵树涂料股份有限公司 | Sulfide-based metallic lead ion colorimetric detection method |
| WO2016019217A1 (en) * | 2014-07-31 | 2016-02-04 | Duquesne University Of The Holy Ghost | Compositions, methods and devices thereof for fluorescent analysis of gunshot residue |
| CN105510315A (en) * | 2015-11-27 | 2016-04-20 | 蒋小良 | Analytical method for measuring content of soluble lead in domestic ceramic |
| CN106770261A (en) * | 2017-02-08 | 2017-05-31 | 北京爱富迪医药科技发展有限公司 | A kind of kit for water sample lead field quick detection |
-
2017
- 2017-11-24 CN CN201711188679.1A patent/CN107976438A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203758918U (en) * | 2014-04-15 | 2014-08-06 | 安徽新华学院 | Device for joint detection on three heavy metal ions in cosmetics |
| CN103983642A (en) * | 2014-06-03 | 2014-08-13 | 三棵树涂料股份有限公司 | Sulfide-based metallic lead ion colorimetric detection method |
| WO2016019217A1 (en) * | 2014-07-31 | 2016-02-04 | Duquesne University Of The Holy Ghost | Compositions, methods and devices thereof for fluorescent analysis of gunshot residue |
| CN105510315A (en) * | 2015-11-27 | 2016-04-20 | 蒋小良 | Analytical method for measuring content of soluble lead in domestic ceramic |
| CN106770261A (en) * | 2017-02-08 | 2017-05-31 | 北京爱富迪医药科技发展有限公司 | A kind of kit for water sample lead field quick detection |
Non-Patent Citations (1)
| Title |
|---|
| 吴孝仙等: "《分析规程（汇编）》", 31 December 1979 * |
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