CN106770261A - A kind of kit for water sample lead field quick detection - Google Patents
A kind of kit for water sample lead field quick detection Download PDFInfo
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- CN106770261A CN106770261A CN201710069104.1A CN201710069104A CN106770261A CN 106770261 A CN106770261 A CN 106770261A CN 201710069104 A CN201710069104 A CN 201710069104A CN 106770261 A CN106770261 A CN 106770261A
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- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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Abstract
The invention discloses a kind of kit for water sample lead field quick detection, it includes：Lead detection reagent, lead Test paper, lead standard color card and consumptive material, independently pack, wherein, lead detection reagent includes hydrochloric acid eluent and NaOH neutralizer, lead Test paper is sulfydryl dithizone test paper, lead standard color card is photochrome of the 10 μ l lead standard liquids after chromogenic reaction on sulfydryl dithizone test paper, and consumptive material includes sulfydryl absorbent filter paper, water sampling suction pipe, outer box, filtering FirebirdTM, liquid-transfering sucker, waste liquid pool and wash-out plate.The present invention is advantageous in that：(1) operating process can be greatly simplified, shortens detection time, detection can be completed in 30min, the sampling location fast qualitative for realizing lead is determined；(2) equipment and the high-tech personnel of costliness need not be equipped with；(3) sensitivity, stability, the reliability of detection reach the requirement of evaluation of hygiene standard；(4) test paper, consumptive material and reagent are independently packed, and are convenient for carrying.
Description
Technical field
The present invention relates to a kind of detection kit, and in particular to a kind of kit for water sample lead field quick detection,
Belong to chemical analysis detection technique field.
Background technology
Due to the mankind to the exploitation of lead, smelt and products containing lead is widely used, such as paint, coating, printing, electronics product
The activities such as product, five metalworkings and battery manufacture are increasing, the discharge of factory's three wastes and the discharge of vehicle exhaust, cause a large amount of
Lead dust and lead-containing compounds enter big gas and water and soil, easily cause the pollution of living environment.
Lead is a Heavy Metallic Elements, it is impossible to be biodegradable, and lead dust or lead-containing compounds are largely accumulated in local environment
It is easy to directly be caused lead poisoning event by human consumption.Lead dust or lead-containing compounds are accumulated for a long time in local environment, by rice
The dense product of the animals such as the crops such as paddy and wheat, aquatic products and poultry, is eaten by the mankind indirectly, causes chronic accumulation and poisoning.Cause
This, in active detection environment especially in water sample lead presence, be important means that mankind's preventing and treating lead is damaged.
At present, in detection water sample there be the method for lead：Atomic absorption method, Differential Potentiometric Stripping Analysis with Experimental, polarography, gas-chromatography
Method, AAS etc..
Though these methods are sensitive, accurate, generally existing pre-treatment is cumbersome, required instrument and equipment is expensive, detection time compared with
The problems such as long, detection technique requires high, it is difficult to the scene for being applied to the environmental and biological samples such as food, water sample, urine is fast
Speed detection.
The content of the invention
It is solve the deficiencies in the prior art, it is an object of the invention to provide a kind of for water sample lead field quick detection
Kit.
In order to realize above-mentioned target, the present invention is adopted the following technical scheme that：
A kind of kit for water sample lead field quick detection, it is characterised in that including：Lead detection reagent, lead detection
Test paper, lead standard color card and consumptive material, independently pack, wherein,
Foregoing lead detection reagent includes：Hydrochloric acid eluent and NaOH neutralizer, wherein, hydrochloric acid eluent is O.1M salt
Acid solution, NaOH neutralizer is O.4M sodium hydroxide solution；
Foregoing lead Test paper is：Sulfydryl dithizone test paper；
Foregoing lead standard color card is：Colored photograph of the 10 μ l lead standard liquids after chromogenic reaction on sulfydryl dithizone test paper
Piece；
Foregoing consumptive material includes：It is sulfydryl absorbent filter paper, water sampling suction pipe, outer box, filtering FirebirdTM, liquid-transfering sucker, useless
Liquid pool and wash-out plate, wherein, filtering FirebirdTM is the plastic pallet for being provided with 2ml suction pipette heads, liquid-transfering sucker according to
Way is divided into 250 μ l hydrochloric acid eluents suction nozzles, 250 μ l NaOH neutralizer suction nozzles and 10 μ l point sample suction nozzles.
It is previously described for the kit of water sample lead field quick detection, it is characterised in that aforementioned sulfhydryl dithizone test paper
Preparation method is：
By the drying of quantitative analysis filter paper, the quantitative analysis filter paper immersion thioglycolic containing 300ml-400ml of drying per 100g
Acid, the acetic acid that 200ml-300ml acetic anhydride, 90ml-120ml concentration are 36v/v%, the 0.8ml-1.2ml concentrated sulfuric acids and 25ml-
In the solution of 35ml deionized waters, 96h is placed in 37 DEG C of -39 DEG C of electric heating constant temperature close drying casees, deionized water is used after taking-up
Neutrality is washed till, is finally squeezed and is removed moisture and dried in 37 DEG C of -39 DEG C of Constant Temp. Ovens to obtain sulfydryl absorbent filter paper, will dried
Dry sulfydryl absorbent filter paper is immersed in the dithizone chloroformic solution that concentration is 0.5g/L, soaks 1min-5min, is taken out after the moon
The dry place of airing dries, black polybag lucifuge sealing preserve.
It is previously described for the kit of water sample lead field quick detection, it is characterised in that the system of aforementioned sulfhydryl absorbent filter paper
Preparation Method is：
By the drying of quantitative analysis filter paper, the quantitative analysis filter paper immersion thioglycolic containing 300ml-400ml of drying per 100g
Acid, 200ml-300ml acetic anhydride, 90ml-120ml concentration are the acetic acid 0.8ml-1.2ml concentrated sulfuric acids and 25ml- of 36v/v%
In the solution of 35ml deionized waters, 96h is placed in 37 DEG C of -39 DEG C of electric heating constant temperature close drying casees, deionized water is used after taking-up
Neutrality is washed till, is finally squeezed and is removed moisture and dried in 37 DEG C of -39 DEG C of Constant Temp. Ovens.
It is previously described for the kit of water sample lead field quick detection, it is characterised in that foregoing water sampling suction pipe, 250 μ
On l hydrochloric acid eluents suction nozzle, 250 μ l NaOH neutralizers suction nozzles, 10 μ l point samples suction nozzles, wash-out plate and filtering FirebirdTM
2ml suction pipette heads, it is preceding all first with 3% nitric acid dousing 12h using, then with deionized water rinsing to neutrality, last 65 DEG C
Temperature control is dried.
It is previously described for the kit of water sample lead field quick detection, it is characterised in that on foregoing lead standard color card, it is purple
Blue colour developing round spot is arranged in order from small to large, the lead content of corresponding lead standard liquid be followed successively by 1.0mg/L, 1.2mg/L,
1.4mg/L, 1.6mg/L, 1.8mg/L, 2.0mg/L, 3.0mg/L, 4.0mg/L and 5.0mg/L.
It is previously described for the kit of water sample lead field quick detection, it is characterised in that application method is as follows：
(1) the 2ml suction pipette heads that will be arranged on filtering adsorption support are corresponded simultaneously with the reacting hole on wash-out plate
It is numbered, then sulfydryl absorbent filter paper is folded tapered and inserted installed in the 2ml pipettors suction filtered on adsorption support
It is standby in head；
(2) using deionized water as blank, sample 1ml successively from multiple water samples of doubtful lead contamination and instil right
In the sulfydryl absorbent filter paper that should be numbered, filtering removes waste liquid pool after finishing, and blots each 2ml on filtering adsorption support with filter paper and moves
Raffinate under liquid device suction nozzle, wash-out plate is laid in the case where adsorption support is filtered, and makes each 2ml suction pipette heads on filtering adsorption support
Point is aligned one by one with the reacting hole on wash-out plate；
(3) sulfydryl absorbent filter paper is eluted with hydrochloric acid eluent, drop drenches 100 μ l every time, and co-elute is twice；
(4) wash-out plate is taken out, adds the μ l of NaOH neutralizer 50 to be neutralized in reacting hole, vibration shakes up；
(5) the μ l of each sample 10 are drawn successively with micropipettor, is dropped on the sulfydryl dithizone test paper of advance numbering, observation
Expansion and chromogenic reaction of each sample on sulfydryl dithizone test paper, finally by sulfydryl dithizone test paper and lead standard color card ratio
Color, judges the content of lead in each water sample accordingly, and data retention is carried out by digital photography.
The present invention is advantageous in that：
(1) when detecting water sample lead using detection kit of the invention, operating process can be greatly simplified, when shortening detection
Between, detection can be completed in 30min, the sampling location fast qualitative for realizing lead is determined, and measurement result can be used as preliminary judgement
The important reference of event property, is particularly suited for the live qualitative determination of lead in contaminant water sample and urine；
(2) when detecting water sample lead using detection kit of the invention, it is not necessary to be equipped with expensive equipment and high-tech people
Member, it is to avoid the waste of man power and material；
(3) when detecting water sample lead using detection kit of the invention, the sensitivity of detection, stability, reliability reach
To the requirement of evaluation of hygiene standard, minimum detectability is 1.0mg/L；
(4) test paper, consumptive material and reagent are independently packed, and are convenient for carrying, and usage cycles are long.
Brief description of the drawings
Fig. 1 is the structural representation of water sampling suction pipe；
Fig. 2 is the structural representation of outer box；
Fig. 3 is the structural representation for filtering FirebirdTM；
Fig. 4 is the structural representation of liquid-transfering sucker；
Fig. 5 is the structural representation of waste liquid pool；
Fig. 6 is the structural representation for eluting plate；
Fig. 7 is to make the lead standard color card for obtaining.
Specific embodiment
Make specific introduction to the present invention below in conjunction with the drawings and specific embodiments.
First, sulfydryl absorbent filter paper
Sulfydryl absorbent filter paper is the quantitative analysis filter paper processed through sulfhydrylation, for filtering, adsorbing and eluting water sample sample.
The preparation method of sulfydryl absorbent filter paper：
By quantitative analysis filter paper drying, per 100g drying quantitative analysis filter paper immersion containing 300ml (can properly increase to
400ml) thioglycolic acid, 200ml (can properly increase to 300ml) acetic anhydride, 90ml (can properly increase to 120ml) concentration are
The acetic acid of 36v/v%, 0.8ml (can properly increase to the 1.2ml) concentrated sulfuric acids and 25ml (can properly increase to 35ml) deionized water
Solution in, place 96h in 37 DEG C of -39 DEG C of electric heating constant temperature close drying casees, be washed with deionized water after taking-up to neutrality, finally
Squeeze and remove moisture and dried in 37 DEG C of -39 DEG C of Constant Temp. Ovens.
2nd, lead Test paper
Lead Test paper is sulfydryl dithizone test paper.
The preparation method of sulfydryl dithizone test paper：
The sulfydryl absorbent filter paper dried that previous step is prepared is immersed in the dithizone chloroform that concentration is 0.5g/L
In solution, immersion 1min (can proper extension to 5min), taking-up is dried after the cool place place of drying, and black polybag lucifuge sealing is protected
Deposit.
3rd, lead detection reagent
Lead detection reagent includes：Hydrochloric acid eluent and NaOH neutralizer, the two is respectively loaded in reagent bottle.
Hydrochloric acid eluent is O.1M hydrochloric acid solution, is obtained using conventional method.
NaOH neutralizer is O.4M sodium hydroxide solution, is obtained using conventional method.
4th, other consumptive materials
Water sampling suction pipe：The Dispoable medical 2ml plastics for indicating tetra- scales of 0.5ml, 1.0ml, 1.5ml and 2ml are inhaled
Pipe, for gathering water sample, as shown in Figure 1.
Outer box：The box body of copperplate box body made of paper or plastic material, for placing filtering FirebirdTM, as shown in Figure 2.
Filtering FirebirdTM：The plastic pallet of 2ml suction pipette heads is installed, as shown in figure 3, wherein, 2ml pipettors are inhaled
Head completes filtering, absorption and the wash-out of water sample sample for supporting sulfydryl absorbent filter paper.
Liquid-transfering sucker：Dispoable medical suction pipette head, as shown in figure 4, be divided into 250 μ l hydrochloric acid eluents according to purposes inhaling
Head, 250 μ l NaOH neutralizer suction nozzles and 10 μ l point sample suction nozzles.
Waste liquid pool：Plastics square box, can be placed under filtering FirebirdTM, for collecting filtering waste liquid, as shown in Figure 5.
Wash-out plate：Porous plastics PCR reaction plates, for collecting eluent, as shown in Figure 6.
Water sampling suction pipe, 250 μ l hydrochloric acid eluents suction nozzles, 250 μ l NaOH neutralizers suction nozzles, 10 μ l point samples are inhaled
2ml suction pipette heads on head, wash-out plate and filtering FirebirdTM, using preceding all first with 3% nitric acid dousing 12h, Ran Houyong
Deionized water rinsing to neutrality, dry by last 65 DEG C of temperature controls.
5th, lead standard color card
Lead standard color card is photochrome of the 10 μ l lead standard liquids after chromogenic reaction on sulfydryl dithizone test paper, is such as schemed
Shown in 7.
The preparation method of lead standard color card：
The sulfydryl for being inserted into taper folding in the multiple 2ml suction pipette heads on filtering FirebirdTM adsorbs filter
Paper, and number consecutively, the lead concentration that will be prepared respectively 0.4mg/L, 1.0mg/L, 1.2mg/L, 1.4mg/L, 1.8mg/L,
The acetic acid lead solution of 2.0mg/L, 3.0mg/L, 4.0mg/L and 5.0mg/L respectively takes 1ml, instils inhaled in numbered sulfydryl respectively
The waste liquid pool under filtering FirebirdTM is removed in attached filter paper, after filtering, each pipettor on filtering FirebirdTM is blotted with filter paper and is inhaled
Raffinate under head, wash-out plate is laid according to numbering under each suction pipette head, must make suction pipette head point alignment wash-out plate
Reacting hole, add hydrochloric acid eluent at twice in each sulfydryl absorbent filter paper afterwards, every time plus 100 μ l, wash-out finish after take
Go out to elute plate, the μ l of NaOH neutralizer 50 are added in each reacting hole, vibration shakes up, 10 μ l then drawn successively and drops in mercapto
On base dithizone test paper, expansion and chromogenic reaction of each sample on sulfydryl dithizone test paper are observed, made by digital photography
Go out lead standard color card.
Reference picture 7, on lead standard color card, indigo colour developing round spot is arranged in order from small to large, corresponding lead standard
The lead content of solution be followed successively by 1.0mg/L, 1.2mg/L, 1.4mg/L, 1.6mg/L, 1.8mg/L, 2.0mg/L, 3.0mg/L,
4.0mg/L and 5.0mg/L.
6th, Cleaning Principle
Lead ion in water sample is adsorbed and concentration by sulfydryl absorbent filter paper, molten through a small amount of hydrochloric acid solution wash-out and NaOH
In liquid with after, developed the color easily on sulfydryl dithizone test paper, lead content is more, indigo colour developing round spot is bigger, by colour developing
The area or average diameter of round spot can in preliminary judgement sample lead content.
7th, detection method
Embodiment 1：The quick detection of heavy metal lead in multiple pollution water samples
The 2ml suction pipette heads that will be arranged on filtering adsorption support are corresponded and carried out with the reacting hole on wash-out plate
Numbering, then folds sulfydryl absorbent filter paper tapered and inserts in the 2ml suction pipette heads being arranged on filtering adsorption support,
It is standby.
Using deionized water as blank, sample 1ml successively from multiple water samples of doubtful lead contamination and instil in correspondence
In the sulfydryl absorbent filter paper of numbering, filtering removes waste liquid pool after finishing, and each 2ml liquid reliefs on filtering adsorption support are blotted with filter paper
Raffinate under device suction nozzle, wash-out plate is laid in the case where adsorption support is filtered, and makes each 2ml suction pipette heads point on filtering adsorption support
It is aligned one by one with the reacting hole on wash-out plate.
Sulfydryl absorbent filter paper is eluted with hydrochloric acid eluent afterwards, drop drenches 100 μ l every time, and co-elute is twice.Pass through
Wash-out plate is repeatedly adherent with suction nozzle point, it is ensured that each reaction tube eluent is collected complete.Wash-out plate is taken out, hydrogen is added in reacting hole
The μ l of sodium oxide molybdena neutralizer 50 are neutralized, and vibration shakes up, and draw the μ l of each sample 10 successively with micropipettor, drop in numbering in advance
Sulfydryl dithizone test paper on, observe the expansion and chromogenic reaction of each sample on sulfydryl dithizone test paper, it is finally that sulfydryl is double
Sulphur hydrazone test paper and lead standard color card colorimetric, judge the content of lead in each water sample accordingly, and data retention is carried out by digital photography.
The whole time-consuming 28min of detection process.
Embodiment 2：The detection of professional workman's Determination of Urine Plumbum
The 2ml suction pipette heads that will be arranged on filtering adsorption support are corresponded and carried out with the reacting hole on wash-out plate
Numbering, then folds sulfydryl absorbent filter paper tapered and inserts in the 2ml suction pipette heads of filtering adsorption support, standby
With.
Using deionized water as blank, 1ml instillation is sampled from the urine sample of the professional workman of doubtful lead exposure right
In the sulfydryl absorbent filter paper that should be numbered, filtering removes waste liquid pool after finishing, and blots each 2ml on filtering adsorption support with filter paper and moves
Raffinate under liquid device suction nozzle, wash-out plate is laid in the case where adsorption support is filtered, and makes the reaction on 2ml suction pipette heads point and wash-out plate
Hole is aligned one by one.
Sulfydryl absorbent filter paper is eluted with hydrochloric acid eluent afterwards, drop drenches 100 μ l every time, and co-elute is twice.Pass through
Wash-out plate is repeatedly adherent with suction nozzle point, it is ensured that each reaction tube eluent is collected complete.Wash-out plate is taken out, hydrogen is added in reacting hole
The μ l of sodium oxide molybdena neutralizer 50 are neutralized, and vibration shakes up, and draw the μ l of each sample 10 successively with micropipettor, drop in numbering in advance
Sulfydryl dithizone test paper on, observe the expansion and chromogenic reaction of each sample on sulfydryl dithizone test paper, it is finally that sulfydryl is double
Sulphur hydrazone test paper and lead standard color card colorimetric, judge the content of each Determination of Urine Plumbum accordingly, and data retention is carried out by digital photography.
The whole time-consuming 27min of detection process.
As can be seen here, when detecting water sample lead using detection kit of the invention, detection can be completed in 30min, is realized
Sampling location quick detection lead content.
8th, the evaluation of testing result
5 kits of the invention are taken, the lead standard liquid sample with this 5 kits respectively to 5 various concentrations enters
Row test, daily each sample is determined once respectively with different kits within continuous 5 days, records result, calculates determine knot respectively
The accuracy rate and the coefficient of variation of fruit.
Potassium, sodium, calcium, magnesium, iron, zinc, copper, mercury and the cadmium ion of various concentrations are separately added into lead standard liquid sample,
Observe the influence of these ion pair test results.
After tested it was found that kit of the invention：
(1) sensitivity：More than 95%, minimum detectability is 1.0mg/L.
(2) stability：Deposited 12 months under lucifuge, sealing, dry room temperature environment, the stabilization of kit is good.
(3) reliability：Potassium, sodium in test sample, calcium, magnesium, iron ion do not influence on test result；Zinc, copper, mercury and
Cadmium ion can produce certain influence to test result, by finely tuning the pH value of solution with NaOH neutralizer in 8.5-9.5
Scope, you can improve lead chelate effect, it is ensured that the reliability of detection.
As can be seen here, when detecting water sample lead using detection kit of the invention, the sensitivity of detection, stability, reliability
Property reach the requirement of evaluation of hygiene standard, testing result can be as the preliminary important reference for judging event property, especially
The live qualitative determination of lead suitable for contaminant water sample and urine.
It should be noted that above-described embodiment the invention is not limited in any way, all use equivalents or equivalent change
The technical scheme that the mode changed is obtained, all falls within protection scope of the present invention.
Claims (6)
1. a kind of kit for water sample lead field quick detection, it is characterised in that including：Lead detection reagent, lead detection examination
Paper, lead standard color card and consumptive material, independently pack, wherein,
The lead detection reagent includes：Hydrochloric acid eluent and NaOH neutralizer, wherein, hydrochloric acid eluent is that O.1M hydrochloric acid is molten
Liquid, NaOH neutralizer is O.4M sodium hydroxide solution；
The lead Test paper is：Sulfydryl dithizone test paper；
The lead standard color card is：Photochrome of the 10 μ l lead standard liquids after chromogenic reaction on sulfydryl dithizone test paper；
The consumptive material includes：Sulfydryl absorbent filter paper, water sampling suction pipe, outer box, filtering FirebirdTM, liquid-transfering sucker, waste liquid pool
With wash-out plate, wherein, filtering FirebirdTM is the plastic pallet for being provided with 2ml suction pipette heads, and liquid-transfering sucker is according to purposes point
It is 250 μ l hydrochloric acid eluents suction nozzles, 250 μ l NaOH neutralizer suction nozzles and 10 μ l point sample suction nozzles.
2. the kit for water sample lead field quick detection according to claim 1, it is characterised in that the sulfydryl is double
The preparation method of sulphur hydrazone test paper is：
Quantitative analysis filter paper is dried, the quantitative analysis filter paper immersion thioglycolic acid containing 300ml-400ml of every 100g drying,
200ml-300ml acetic anhydride, 90ml-120ml concentration are acetic acid, the 0.8ml-1.2ml concentrated sulfuric acids and the 25ml-35ml of 36v/v%
In the solution of deionized water, 96h is placed in 37 DEG C of -39 DEG C of electric heating constant temperature close drying casees, be washed with deionized water after taking-up to
Neutrality, finally squeezes and removes moisture and dried in 37 DEG C of -39 DEG C of Constant Temp. Ovens to obtain sulfydryl absorbent filter paper, by what is dried
Sulfydryl absorbent filter paper is immersed in the dithizone chloroformic solution that concentration is 0.5g/L, soaks 1min-5min, takes out dry after cool place
Dry place dries, black polybag lucifuge sealing preserve.
3. the kit for water sample lead field quick detection according to claim 1, it is characterised in that the sulfydryl is inhaled
The preparation method of attached filter paper is：
Quantitative analysis filter paper is dried, the quantitative analysis filter paper immersion thioglycolic acid containing 300ml-400ml of every 100g drying,
200ml-300ml acetic anhydride, 90ml-120ml concentration are gone for the acetic acid 0.8ml-1.2ml concentrated sulfuric acids and 25ml-35ml of 36v/v%
In the solution of ionized water, 96h is placed in 37 DEG C of -39 DEG C of electric heating constant temperature close drying casees, be washed with deionized water into after taking-up
Property, finally squeeze and remove moisture and dried in 37 DEG C of -39 DEG C of Constant Temp. Ovens.
4. the kit for water sample lead field quick detection according to claim 1, it is characterised in that the water sample is adopted
Collection suction pipe, 250 μ l hydrochloric acid eluents suction nozzles, 250 μ l NaOH neutralizers suction nozzles, 10 μ l point samples suction nozzles, wash-out plate and filtering
2ml suction pipette heads on FirebirdTM, it is preceding all first with 3% nitric acid dousing 12h using, then with deionized water rinsing into
Property, last 65 DEG C of temperature controls drying.
5. the kit for water sample lead field quick detection according to claim 1, it is characterised in that in the lead mark
On quasi- colour atla, indigo colour developing round spot is arranged in order from small to large, and the lead content of corresponding lead standard liquid is followed successively by
1.0mg/L, 1.2mg/L, 1.4mg/L, 1.6mg/L, 1.8mg/L, 2.0mg/L, 3.0mg/L, 4.0mg/L and 5.0mg/L.
6. the kit for water sample lead field quick detection according to claim 1, it is characterised in that application method is such as
Under：
(1) the 2ml suction pipette heads that will be arranged on filtering adsorption support are corresponded and carried out with the reacting hole on wash-out plate
Numbering, then folds sulfydryl absorbent filter paper tapered and inserts in the 2ml suction pipette heads being arranged on filtering adsorption support,
It is standby；
(2) using deionized water as blank, sample 1ml successively from multiple water samples of doubtful lead contamination and instil in correspondence volume
Number sulfydryl absorbent filter paper in, filtering finish after remove waste liquid pool, with filter paper blot filtering adsorption support on each 2ml pipettors
Raffinate under suction nozzle, filter adsorption support under lay wash-out plate, make on filtering adsorption support each 2ml suction pipette heads point with
Reacting hole on wash-out plate is aligned one by one；
(3) sulfydryl absorbent filter paper is eluted with hydrochloric acid eluent, drop drenches 100 μ l every time, and co-elute is twice；
(4) wash-out plate is taken out, adds the μ l of NaOH neutralizer 50 to be neutralized in reacting hole, vibration shakes up；
(5) the μ l of each sample 10 are drawn successively with micropipettor, is dropped on the sulfydryl dithizone test paper of advance numbering, observe various kinds
This expansion and chromogenic reaction on sulfydryl dithizone test paper, finally by sulfydryl dithizone test paper and lead standard color card colorimetric, according to
This judges the content of lead in each water sample, and data retention is carried out by digital photography.
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| CN113495086A (en) * | 2020-04-03 | 2021-10-12 | 深圳市帝迈生物技术有限公司 | POCT blood cell analyzer and kit |
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