CN107976414A - A kind of portable instrument and the method for detecting tea polyphenols - Google Patents
A kind of portable instrument and the method for detecting tea polyphenols Download PDFInfo
- Publication number
- CN107976414A CN107976414A CN201711105101.5A CN201711105101A CN107976414A CN 107976414 A CN107976414 A CN 107976414A CN 201711105101 A CN201711105101 A CN 201711105101A CN 107976414 A CN107976414 A CN 107976414A
- Authority
- CN
- China
- Prior art keywords
- tea polyphenols
- light source
- instrument
- detection
- module
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 34
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 34
- 241001122767 Theaceae Species 0.000 title claims abstract 8
- 238000000034 method Methods 0.000 title claims description 13
- 238000001514 detection method Methods 0.000 claims abstract description 32
- 239000002002 slurry Substances 0.000 claims description 10
- 238000012360 testing method Methods 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 5
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 238000002835 absorbance Methods 0.000 abstract description 9
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 abstract description 5
- 239000012490 blank solution Substances 0.000 abstract description 4
- 230000003287 optical effect Effects 0.000 abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 3
- 238000002798 spectrophotometry method Methods 0.000 abstract description 3
- 244000269722 Thea sinensis Species 0.000 description 37
- 235000013616 tea Nutrition 0.000 description 32
- 235000009569 green tea Nutrition 0.000 description 6
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 5
- 240000003553 Leptospermum scoparium Species 0.000 description 5
- 235000015459 Lycium barbarum Nutrition 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 235000006468 Thea sinensis Nutrition 0.000 description 4
- 235000020279 black tea Nutrition 0.000 description 4
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000003750 conditioning effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229940074391 gallic acid Drugs 0.000 description 2
- 235000004515 gallic acid Nutrition 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 238000004497 NIR spectroscopy Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
Abstract
A kind of portable tea polyphenols detecting instrument, including closed chamber and detection module；It is followed successively by inside closed chamber：Light source, cuvette, Photoelectric Detection module, modular converter, control module, display module；The light source includes the ultraviolet source for producing 290nm and the visible light source LD for producing 650nm.Principle with instrument detection polyphenol content is to use ultraviolet spectrophotometry, compared with GB/T8313, it is not necessary to add forint phenol reagent, it is not necessary to which blank solution contrasts, using the tea polyphenols of various concentrations to the difference of the ultraviolet optical absorbance of specific wavelength, the quick detection to tea polyphenols concentration is realized.
Description
Technical field
The invention belongs to technical field of biological, and in particular to a kind of method with instrument detection tea polyphenols.
Background technology
In order to select the tea tree improved seeds for fitting black tea processed, it is necessary on the basis of field test, using while screen, while
Expand the method that numerous, side carries out regional testing and natomical leaf structure identification, select the excellent tea tree breed for fitting black tea processed, and it is right
Phenological period, yield, quality and the resistance of selected type strain synchronize identification, and the breed breeding cycle was 10 or so years.Cause
This, in field test, it is necessary to know the content of tea polyphenols in tender leaf.The total quantity measuring method of tea polyphenols mainly includes spectrophotometric
Method, near infrared spectroscopy, electrochemical methods, fluorescence detection etc..And GB/T8313《Tea Polyphenols in Tea and catechin contain
The detection method of amount》For visible spectrophotometry-forint phenol colorimetric method.But these methods are required in laboratory by professional
Carry out, complex steps, the time is long, testing cost is high.Therefore need a kind of portable instrumentation, can field quick detection it is new
The content of tea polyphenols in fresh tea passes, helps tea tree breed quickly to screen, realizes tea tree breed quick breeding.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of Portable rapid detector device, and quick with the instrument
The method for detecting tea polyphenols.
In order to solve the technical problem, the present invention devises a kind of instrument of portable quick detection polyphenol content.Inspection
The principle of survey method uses ultraviolet spectrophotometry, and using the ultraviolet absorption characteristic of tea polyphenols, the tea polyphenols of various concentrations are to spy
The difference of the ultraviolet optical absorbance of standing wave length, realizes the quick detection to tea polyphenols concentration.Compared with GB/T8313, it is not necessary to add
Add forint phenol reagent, it is not necessary to make blank solution sample.
Whole instrument includes light source module, sample cell, Photoelectric Detection module, modular converter, microcontroller, display module.
The light source module includes ultraviolet source module and visible light source module, and it is special that the ultraviolet source produces 290nm
The ultraviolet light of standing wave length, for detecting the absorbance of tea polyphenols；The visible light source module contrasts visible optical module for LD, produces
The visible light source of 650nm, for substituting blank solution sample in GB/T8313 methods, eliminates background interference；
The sample cell places tealeaves slurries to be tested；
The Photoelectric Detection module is made of photodetection pipe and signal conditioning circuit, and photodetection pipe converts optical signals to electricity
Signal, and be amplified by signal conditioning circuit, filter after be converted into standard analog electric signal；For detecting ultraviolet ray intensity
Visual intensity is contrasted with LD；
The analog electrical signal that Photoelectric Detection module exports is converted to digital signal so that microcontroller is into one by the modular converter
Step processing；
The digital signal that the microcontroller exports modular converter is handled, calculated and further controls display module；
The display module shows the data of test, analysis.
Using the portable instrument, the step of detecting Tea Polyphenols in Tea, is as follows：
Step 1：Instrument is opened
（1）Power on；Instrument returns to zero；
Step 2：Tea Samples pre-process
（1）Tealeaves cleans；
（2）Tealeaves is made by slurries by beater, is stood；
Step 3：The detection of sample to be tested
（1）The supernatant of tealeaves slurries is gone to be put into sample cell；
（2）Sample cell is put into instrument；
（3）Tealeaves slurries are tested；
（4）Show tea polyphenols test result.
The present invention has the following advantages that compared with prior art：
1 compares with national standard, and the present invention need not add forint phenol reagent, and change Detection wavelength is 290nm.The instrument is taken
Band is convenient, easy to field work.National Standard Method measure a sample take around 1.5 it is small when, and this method time is a few minutes, greatly
Detection time is shortened greatly, can be applicable to the quick measure in tea tree breed quick breeding and tea products production process.
2nd, this patent is different to the absorbance of ultraviolet light using the tea polyphenols solution of various concentrations, and visible ray is not inhaled
Receive, the absorbance of visible ray is subtracted by the absorbance of the ultraviolet light of tea polyphenols solution, achieve the purpose that to eliminate background interference.This
Patent utilizes the physicochemical property and rapid detection apparatus organic assembling of tea polyphenols, realizes that tea polyphenols quickly detect, and instrument can industry
Metaplasia is produced.
Brief description of the drawings
Fig. 1 instrument composition schematic diagrams
No. 6 Fig. 2 tea polyphenols, gallic acid, black tea, green tea 1, green tea 3, green tea sample all band scanning figures.
Embodiment
With reference to embodiment, the present invention is described in detail.The embodiment is only the preferable reality to the present invention
Mode is applied, not makees limitation in any form to the present invention, every technical spirit according to the present invention is implemented to more than
The simple modification that mode is made, equivalent variations and modification, belong in the range of technical solution of the present invention.
Fig. 1 is instrumentation diagram, including closed chamber and detection module, is followed successively by inside the closed chamber：Light source（1）, sample
Product pond（2）, Photoelectric Detection module（3）, modular converter（4）, control module（5）, display module（6）.Light source（1）Including ultraviolet light
Light source and visible light source LD；Ultraviolet source produces 290nm ultraviolet lights, it is seen that radiant LD produces 650nm visible rays.It is different
The tea polyphenols solution of concentration is different to the absorbance of the ultraviolet light of 290nm, and the visible ray of 650nm is not absorbed, more by tea
The absorbance of the ultraviolet light of the 290nm of phenol solution subtracts the absorbance of 650nm visible rays, achievees the purpose that to eliminate background interference.
When tea polyphenols detect, the ultraviolet source of 290nm and the visible light source of 650nm are opened, while irradiates tea polyphenols sample, detection is passed through
The light intensity of the 290nm ultraviolet lights of tea polyphenols sample and the visible ray of 650nm, by resolving, eliminates background interference, for substituting
Blank solution sample effect in GB/T8313；Photoelectric Detection module（3）It is used for detecting ultraviolet light and visible for model LSGPD-UV1.2
Luminous intensity；Modular converter（4）It is used for the analog signal of the output of Photoelectric Detection module to be converted into digital letter for model AD7715
Number；Control module（5）For model STC89C52RC, for detection signal being resolved and being controlled the work of light source module.It is aobvious
Show module（6）For model LCD1602, detection, calculation result are shown.
The step of specifically detecting tea polyphenols using the instrument is as follows：
Step 1：Instrument is opened
Power on, while open light source（1）In 290nm ultraviolet sources and 650nm visible light sources；Instrument returns to zero, and shows mould
Block（6）For 0；
Step 2：Fresh tea leaf sample treatment
1）Tealeaves cleans；
2）Tealeaves is made by slurries by beater, is stood；
Step 3：The detection of sample to be tested
1）Take tealeaves slurries to obtain supernatant and be put into sample cell（2）In；
2）Sample cell（2）It is put into instrument；
3）UV absorption test is carried out to the supernatant of tealeaves slurries；By Photoelectric Detection module（3）Detect ultraviolet light and visible
Luminous intensity, modular converter（4）The analog signal of the output of Photoelectric Detection module is converted into digital signal；Control module（5）It is right
Detection signal is resolved and is controlled the work of light source module；
4）Display module（6）Show tea polyphenols test result；
No. 6 tea polyphenols, gallic acid, black tea, green tea 1, green tea 3, green tea samples are used into national standard GB/T8313 methods respectively
Detection, is then diluted to a certain concentration, then is scanned respectively with all band, and scanning result is shown in Fig. 2, the display module of instrument（6）'s
It the results are shown in Table 1.
Claims (2)
- A kind of 1. portable tea polyphenols detecting instrument, it is characterised in that：Including closed chamber and detection module；Inside the closed chamber It is followed successively by：Light source（1）, sample cell（2）, Photoelectric Detection module（3）, modular converter（4）, control module（5）, display module（6）； The light source（1）Including producing 290nm ultraviolet sources and 650nm visible light sources LD.
- 2. with the method for the detecting instrument detection tea polyphenols described in claim 1, it is characterised in that：Include the following steps：Step 1：Instrument is openedPower on, while open light source（1）In 290nm ultraviolet sources and 650nm visible light sources, instrument zeroing；Step 2：Fresh tea leaf sample treatment1）Tealeaves cleans；2）Tealeaves is made by slurries by beater, is stood；Step 3：The detection of sample to be tested1）Take tealeaves slurries to obtain supernatant and be put into sample cell（2）In；2）Sample cell（2）It is put into instrument；3）Open light source at the same time（1）In 290nm ultraviolet sources and 650nm visible light sources；The supernatant of tealeaves slurries is carried out UV absorption is tested；4）Display module（6）Show tea polyphenols test result.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711105101.5A CN107976414A (en) | 2017-11-10 | 2017-11-10 | A kind of portable instrument and the method for detecting tea polyphenols |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711105101.5A CN107976414A (en) | 2017-11-10 | 2017-11-10 | A kind of portable instrument and the method for detecting tea polyphenols |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107976414A true CN107976414A (en) | 2018-05-01 |
Family
ID=62013282
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711105101.5A Pending CN107976414A (en) | 2017-11-10 | 2017-11-10 | A kind of portable instrument and the method for detecting tea polyphenols |
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| Country | Link |
|---|---|
| CN (1) | CN107976414A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2578496A (en) * | 2018-10-26 | 2020-05-13 | Univ Hebei Technology | Method for detecting easy-cleaning property of ceramic |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103234934A (en) * | 2013-03-29 | 2013-08-07 | 江汉大学 | Method for determining fat soluble green tea polyphenol content |
| CN105343335A (en) * | 2015-11-17 | 2016-02-24 | 南昌大学 | Method for optimizing extraction technology of aquilaria sinensis leaf tea polyphenol by utilizing response surface process |
-
2017
- 2017-11-10 CN CN201711105101.5A patent/CN107976414A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103234934A (en) * | 2013-03-29 | 2013-08-07 | 江汉大学 | Method for determining fat soluble green tea polyphenol content |
| CN105343335A (en) * | 2015-11-17 | 2016-02-24 | 南昌大学 | Method for optimizing extraction technology of aquilaria sinensis leaf tea polyphenol by utilizing response surface process |
Non-Patent Citations (2)
| Title |
|---|
| 朱丹: "利用光学-电化学技术快速测定茶多酚含量的方法和装置的研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 韩立 等: "《仪器分析》", 31 October 2014, 吉林大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2578496A (en) * | 2018-10-26 | 2020-05-13 | Univ Hebei Technology | Method for detecting easy-cleaning property of ceramic |
| GB2578496B (en) * | 2018-10-26 | 2021-03-10 | Univ Hebei Technology | Method for detecting easy-cleaning property of ceramic |
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| PB01 | Publication | ||
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Application publication date: 20180501 |