CN107024449B - The other near-infrared spectral analytical method of mammalian is differentiated based on urine - Google Patents
The other near-infrared spectral analytical method of mammalian is differentiated based on urine Download PDFInfo
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- CN107024449B CN107024449B CN201610673315.1A CN201610673315A CN107024449B CN 107024449 B CN107024449 B CN 107024449B CN 201610673315 A CN201610673315 A CN 201610673315A CN 107024449 B CN107024449 B CN 107024449B
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- 210000002700 urine Anatomy 0.000 title claims abstract description 39
- 230000003595 spectral effect Effects 0.000 title claims abstract description 23
- 238000004458 analytical method Methods 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 20
- 238000001228 spectrum Methods 0.000 claims abstract description 12
- 238000004611 spectroscopical analysis Methods 0.000 claims abstract description 7
- 238000002235 transmission spectroscopy Methods 0.000 claims description 7
- 238000000513 principal component analysis Methods 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 3
- 238000013210 evaluation model Methods 0.000 claims description 2
- 241000124008 Mammalia Species 0.000 abstract description 14
- 238000005259 measurement Methods 0.000 abstract description 8
- 238000002329 infrared spectrum Methods 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 4
- 238000007781 pre-processing Methods 0.000 abstract description 2
- 241001465754 Metazoa Species 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000003705 background correction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000012850 discrimination method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3577—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing liquids, e.g. polluted water
Abstract
The invention discloses differentiate the other near-infrared spectral analytical method of mammalian based on urine, first collect the buck urine sample and jenny urine sample of same mammal, the near infrared spectrum of urine sample obtained by acquisition under certain measuring condition, gained spectrum is without pre-processing or carrying out Chemical Measurement pretreatment, the selection modeling spectral region from gained spectroscopic data, after carrying out dimensionality reduction to the data of selected spectral region, the Sex Discrimination model of this kind of mammal is established and verified using chemometrics method；The urine sample for taking this kind of mammal of unknown gender again handles according to the multi-step that method same as before acquires spectrum and carries out spectroscopic data, the Sex Discrimination of this kind of mammal is finally carried out using model built.The present invention is based on the near infrared spectrums of urine to differentiate the gender of mammal in conjunction with chemometric techniques, has the advantages that analysis method is accurate and easy to use, tested body compliance is good.
Description
Technical field
The invention belongs to near-infrared spectral analysis technology fields, are related to a kind of other close based on urine differentiation mammalian
Infrared spectrum analysis.
Background technique
Gender research is one of important content of biology.Mammal is the highest stage of animal development, in lactation
In the ecomanagement of animal, Sex Discrimination is a groundwork of Analysis On Population Dynamics.The mankind are five-star mammals,
The Sex Discrimination of human body is the important content of identification, is had in fields such as personalized service, medical treatment and management of public safety
There is important meaning.Physical examination is a kind of human body Sex Discrimination method of classics, but since it is desired that special inspection place and specific
Inspection personnel make this method have certain limitation.
Summary of the invention
The purpose of the present invention is to provide one kind to differentiate the other near-infrared spectral analytical method of mammalian based on urine,
Have the advantages that analysis method is accurate and easy to use, tested body compliance is good.
Through studying, the invention provides the following technical scheme:
The other near-infrared spectral analytical method of mammalian is differentiated based on urine, comprising the following steps:
(1) the buck urine sample and jenny urine sample of same mammal are collected；
(2) resolution ratio and scanning times of near infrared spectrometer are set, in 10000~4000cm-1In scanning range respectively
The near infrared spectrum of urine sample obtained by acquisition step (1)；
(3) to spectrum obtained by step (2), without pre-processing or carrying out Chemical Measurement pretreatment；
(4) selection modeling spectral region in the spectroscopic data obtained by step (3)；
(5) dimensionality reduction is carried out to the data of spectral region selected by step (4)；
(6) it to step (5) the data obtained, is established using chemometrics method and the gender for verifying this kind of mammal is sentenced
Other model；
(7) urine sample for taking this kind of mammal of unknown gender acquires near infrared light according to step (2) the method
Spectrum is handled according to the multi-step that step (3)~(5) the method carries out spectrum, and then applying step (6) model built is fed
The Sex Discrimination of newborn animal.
When differentiating the gender of mammal, use the urine of mammal as sample, have sample non-invasi, can
Repeated, easily handling advantage.
Preferably, in step (2), the resolution ratio of near infrared spectrometer is set as 2cm-1、4cm-1、8cm-1Or 16cm-1, sweep
Number is retouched to be set as 32,64 or 128 times.
Preferably, in step (3), Chemical Measurement preprocess method is multicomponent signal amendment, standard contact transformation, derivative
With one of smooth or multiple combinations.
Modeling spectral region described in step (4) is 10000~4000cm-1In one or more snippets, can be by modeling software
Automatic screening or artificial screening, can also be on the basis of modeling software automatic screening according to the near-infrared characteristic absorption of analyte
Further artificial optimization.
Preferably, in step (5), dimensionality reduction is carried out using data of the Principal Component Analysis to spectral region selected by step (4).
Preferably, it models the selection gist of number of principal components and adds up variance contribution ratio greater than 85% and step (6) institute for it
The calibration set right judging rate and verifying collection right judging rate for building discrimination model are all larger than 95%.
Preferably, in step (6), Chemical Measurement modeling method is techniques of discriminant analysis.
Preferably, in step (6), the performance of built discrimination model is commented by calibration set right judging rate and verifying collection right judging rate
Valence.
Preferably, the mammal is the mankind.
Preferably, the near-infrared spectral analytical method of human body gender is differentiated based on urine, comprising the following steps:
(1) urine sample of male body and the urine sample of female body are collected；
(2) resolution ratio that near infrared spectrometer is arranged is 8cm-1, scanning times be 64 times, in 10000~4000cm-1It sweeps
Retouch the NIR transmittance spectroscopy of urine sample obtained by difference acquisition step (1) in range；
(3) to spectrum obtained by step (2) without pretreatment；
(4) 9881~4119cm is selected in the spectroscopic data obtained by step (3)-1To model spectral region；
(5) dimensionality reduction is carried out using data of the Principal Component Analysis to spectral region selected by step (4)；
(6) to step (5) the data obtained, to model number of principal components it is 10, is established using techniques of discriminant analysis and verify human body
Sex Discrimination model, with calibration set right judging rate and verifying collection right judging rate evaluation model performance；
(7) urine sample for taking unknown gender human body acquires NIR transmittance spectroscopy according to step (2) the method, presses
The multi-step processing of spectrum is carried out according to step (3)~(5) the method, then applying step (6) model built carries out human body
Sex Discrimination.
The beneficial effects of the present invention are: the present invention is based on the near infrared spectrums of urine to sentence in conjunction with chemometric techniques
The gender of other mammal has the advantages that analysis method is accurate and easy to use, tested body compliance is good.
Detailed description of the invention
Fig. 1 is the near-infrared transmission primary light spectrogram of 10 human body urine samples.
Fig. 2 for the built human body Sex Discrimination model of the present invention differentiation figure.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawing to of the invention preferred
Embodiment is described in detail.
Embodiment 1 is based on urine and differentiates human body gender
1. the collection of urine sample
3 males and 7 women totally 10 trial volunteer middle section urina sanguinis samples are collected respectively, are numbered one by one and record property
Other information.
2. the acquisition of NIR transmittance spectroscopy
Instrument: Antaris II ft-nir spectrometer (Thermo company, the U.S.) is furnished with integrating sphere accessory,
Signal acquisition software is Result3.0.
Spectral measurement condition: resolution ratio 8cm-1, scanning times are 64 times, and scanning range is 10000~4000cm-1。
Spectral measurement method: taking the urine sample of same number appropriate, is placed in the cuvette of a clean dried, upper
State the NIR transmittance spectroscopy of spectral measurement condition measurement urine sample.Each sample repeats to load and measure 5 times.Each sample
Identical measuring condition scanning and background correction are all made of before measurement.
50 near-infrared transmission original spectrums of 10 human body urine samples are as shown in Figure 1.
3. modeling the selection of spectral region
Spectrum not after pretreatment, the modeling spectral region chosen automatically through 8.0 software of TQAnalyst be 9881~
4119cm-1。
4. the dimensionality reduction of spectroscopic data
Using 8.0 software of TQAnalyst, using Principal Component Analysis in 9881~4119cm of selected modeling spectral region-1
Interior to carry out dimensionality reduction to pretreated spectroscopic data, when selected modeling number of principal components is 10, accumulative variance contribution ratio is
99.2%, and the right judging rate of the calibration set of model built and verifying collection is 100.0%.
5. the foundation and verifying of discrimination model
Using the urine sample of collected 2 males and 5 women totally 7 trial volunteers as calibration set sample,
Remaining 1 male and 2 women totally 3 trial volunteers urine sample as verifying collection sample.The above calibration set is used respectively
Sample and verifying collect sample, and to model number of principal components as 10, the Sex Discrimination mould of human body is established and verified using techniques of discriminant analysis
Type.As a result as shown in Fig. 2, the calibration set right judging rate of built discrimination model is 100.0%, verifying collection right judging rate is 100.0%, table
Bright model built has good differentiation performance, is able to use urine sample and accurately differentiates human body gender.
6. the analysis of unknown gender human urine sample
Unknown 1, gender human urine sample is taken, according to the NIR transmittance spectroscopy, right of above-mentioned same procedure acquisition sample
Spectrum carries out multi-step processing, and the human body Sex Discrimination of the urine sample is then carried out using model built, as a result correct display
It is women.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although passing through ginseng
According to the preferred embodiment of the present invention, invention has been described, however, those skilled in the art should understand that, can be in shape
Various changes are made in formula and to it in details, without departing from protection of the invention defined by the appended claims
Range.
Claims (1)
1. differentiating the other near-infrared spectral analytical method of mammalian based on urine, which comprises the following steps:
(1) urine sample of male body and the urine sample of female body are collected；
(2) resolution ratio that near infrared spectrometer is arranged is 8cm-1, scanning times be 64 times, in 10000~4000cm-1Scanning range
The NIR transmittance spectroscopy of urine sample obtained by interior acquisition step (1) respectively；
(3) to spectrum obtained by step (2) without pretreatment；
(4) 9881~4119cm is selected in the spectroscopic data obtained by step (3)-1To model spectral region；
(5) dimensionality reduction is carried out using data of the Principal Component Analysis to spectral region selected by step (4)；
(6) to step (5) the data obtained, to model the property that number of principal components is 10, is established using techniques of discriminant analysis and verify human body
Other discrimination model, with the performance of calibration set right judging rate and verifying collection right judging rate evaluation model；
(7) urine sample for taking unknown gender human body acquires NIR transmittance spectroscopy according to step (2) the method, according to step
Suddenly (3)~(5) the method carries out the multi-step processing of spectrum, and then applying step (6) model built carries out the gender of human body
Differentiate.
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| CN107024449B true CN107024449B (en) | 2019-09-20 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107677634A (en) * | 2017-10-11 | 2018-02-09 | 合肥工业大学 | Method based on clenbuterol hydrochloride in Ftir Spectroscopy detection pig urine |
| CN109632690A (en) * | 2018-12-04 | 2019-04-16 | 孙彤 | Non-intrusion type urine on-line detecting system and the method for establishing prediction model |
Citations (3)
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|---|---|---|---|---|
| CN102145335A (en) * | 2011-03-31 | 2011-08-10 | 浙江省农业科学院 | Automatic photoelectric separator for female and male silkworm eggs |
| CN103472008A (en) * | 2013-08-30 | 2013-12-25 | 黑龙江八一农垦大学 | Gender identification method for chick embryo in near-infrared hatching egg at earlier stage of incubation |
| CN104316473A (en) * | 2014-10-28 | 2015-01-28 | 南京农业大学 | Gender determination method for chicken hatching egg incubation early embryo based on hyperspectral image |
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2016
- 2016-07-29 CN CN201610673315.1A patent/CN107024449B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102145335A (en) * | 2011-03-31 | 2011-08-10 | 浙江省农业科学院 | Automatic photoelectric separator for female and male silkworm eggs |
| CN103472008A (en) * | 2013-08-30 | 2013-12-25 | 黑龙江八一农垦大学 | Gender identification method for chick embryo in near-infrared hatching egg at earlier stage of incubation |
| CN104316473A (en) * | 2014-10-28 | 2015-01-28 | 南京农业大学 | Gender determination method for chicken hatching egg incubation early embryo based on hyperspectral image |
Non-Patent Citations (7)
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| A novel method for human gender classification using Raman spectroscopy of fingernail clippings;Effendi Widjaja et al.;《Analyst》;20080212;第133卷(第4期);第493-498页 * |
| Near infrared spectroscopy applications in amphibian ecology and conservation: gender and species identification;Carrie K. Vance et al.;《NIR news》;20140731;第25卷(第4期);第10-15页 * |
| Near infrared spectroscopy of faeces to evaluate the nutrition and physiology of herbivores;R. Dixon and D. Coates;《J. Near Infrared Spectrosc.》;20081218;第1-31页 * |
| Race Differentiation by Raman Spectroscopy of a Bloodstain for Forensic Purposes;Ewelina Mistek et al;《Anal. Chem.》;20160623;第7453-7456页 * |
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