CN107024448B - The other near-infrared spectral analytical method of mammalian is differentiated based on saliva - Google Patents
The other near-infrared spectral analytical method of mammalian is differentiated based on saliva Download PDFInfo
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- CN107024448B CN107024448B CN201610637980.5A CN201610637980A CN107024448B CN 107024448 B CN107024448 B CN 107024448B CN 201610637980 A CN201610637980 A CN 201610637980A CN 107024448 B CN107024448 B CN 107024448B
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- 210000003296 saliva Anatomy 0.000 title claims abstract description 41
- 230000003595 spectral effect Effects 0.000 title claims abstract description 24
- 238000004458 analytical method Methods 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 20
- 238000001228 spectrum Methods 0.000 claims abstract description 12
- 238000004611 spectroscopical analysis Methods 0.000 claims abstract description 7
- 238000002235 transmission spectroscopy Methods 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 5
- 238000000513 principal component analysis Methods 0.000 claims description 4
- 238000013210 evaluation model Methods 0.000 claims description 2
- 241000124008 Mammalia Species 0.000 abstract description 14
- 238000005259 measurement Methods 0.000 abstract description 8
- 238000002329 infrared spectrum Methods 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 4
- 238000007781 pre-processing Methods 0.000 abstract description 2
- 241001465754 Metazoa Species 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000003705 background correction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000012850 discrimination method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3577—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing liquids, e.g. polluted water
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- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses differentiate the other near-infrared spectral analytical method of mammalian based on saliva, first collect the buck saliva sample and jenny saliva sample of same mammal, the near infrared spectrum of saliva sample obtained by acquisition under certain measuring condition, gained spectrum is without pre-processing or carrying out Chemical Measurement pretreatment, the selection modeling spectral region from gained spectroscopic data, after carrying out dimensionality reduction to the data of selected spectral region, the Sex Discrimination model of this kind of mammal is established and verified using chemometrics method;The saliva sample for taking this kind of mammal of unknown gender again handles according to the multi-step that method same as before acquires spectrum and carries out spectroscopic data, the Sex Discrimination of this kind of mammal is finally carried out using model built.The present invention is based on the near infrared spectrums of saliva to differentiate the gender of mammal in conjunction with chemometric techniques, has the advantages that analysis method is accurate and easy to use, tested body compliance is good.
Description
Technical field
The invention belongs to near-infrared spectral analysis technology fields, are related to a kind of other close based on saliva differentiation mammalian
Infrared spectrum analysis.
Background technique
Gender research is one of important content of biology.Mammal is the highest stage of animal development, in lactation
In the ecomanagement of animal, Sex Discrimination is a groundwork of Analysis On Population Dynamics.The mankind are five-star mammals,
The Sex Discrimination of human body is the important content of identification, is had in fields such as personalized service, medical treatment and management of public safety
There is important meaning.Physical examination is a kind of human body Sex Discrimination method of classics, but since it is desired that special inspection place and specific
Inspection personnel make this method have certain limitation.
Summary of the invention
The purpose of the present invention is to provide one kind to differentiate the other near-infrared spectral analytical method of mammalian based on saliva,
Have the advantages that analysis method is accurate and easy to use, tested body compliance is good.
Through studying, the invention provides the following technical scheme:
The other near-infrared spectral analytical method of mammalian is differentiated based on saliva, comprising the following steps:
(1) the buck saliva sample and jenny saliva sample of same mammal are collected;
(2) resolution ratio and scanning times of near infrared spectrometer are set, in 10000~4000cm-1In scanning range respectively
The near infrared spectrum of saliva sample obtained by acquisition step (1);
(3) to spectrum obtained by step (2), without pre-processing or carrying out Chemical Measurement pretreatment;
(4) selection modeling spectral region in the spectroscopic data obtained by step (3);
(5) dimensionality reduction is carried out to the data of spectral region selected by step (4);
(6) it to step (5) the data obtained, is established using chemometrics method and the gender for verifying this kind of mammal is sentenced
Other model;
(7) saliva sample for taking this kind of mammal of unknown gender acquires near infrared light according to step (2) the method
Spectrum is handled according to the multi-step that step (3)~(5) the method carries out spectrum, and then applying step (6) model built is fed
The Sex Discrimination of newborn animal.
When differentiating the gender of mammal, use the saliva of mammal as sample, have sample non-invasi, can
Repeated, easily handling advantage.
Preferably, in step (2), the resolution ratio of near infrared spectrometer is set as 2cm-1、4cm-1、8cm-1Or 16cm-1, sweep
Number is retouched to be set as 32,64 or 128 times.
Preferably, in step (3), Chemical Measurement preprocess method is multicomponent signal amendment, standard contact transformation, derivative
With one of smooth or multiple combinations.
Modeling spectral region described in step (4) is 10000~4000cm-1In one or more snippets, can be by modeling software
Automatic screening or artificial screening, can also be on the basis of modeling software automatic screening according to the near-infrared characteristic absorption of analyte
Further artificial optimization.
Preferably, in step (5), dimensionality reduction is carried out using data of the Principal Component Analysis to spectral region selected by step (4).
Preferably, it models the selection gist of number of principal components and adds up variance contribution ratio greater than 85% and step (6) institute for it
The calibration set right judging rate and verifying collection right judging rate for building discrimination model are all larger than 95%.
Preferably, in step (6), Chemical Measurement modeling method is techniques of discriminant analysis.
Preferably, in step (6), the performance of built discrimination model is commented by calibration set right judging rate and verifying collection right judging rate
Valence.
Preferably, the mammal is the mankind.
Preferably, the near-infrared spectral analytical method of human body gender is differentiated based on saliva, comprising the following steps:
(1) saliva sample of male body and the saliva sample of female body are collected;
(2) resolution ratio that near infrared spectrometer is arranged is 8cm-1, scanning times be 64 times, in 10000~4000cm-1It sweeps
Retouch the NIR transmittance spectroscopy of saliva sample obtained by difference acquisition step (1) in range;
(3) to spectrum obtained by step (2) without pretreatment;
(4) 9881~4119cm is selected in the spectroscopic data obtained by step (3)-1To model spectral region;
(5) dimensionality reduction is carried out using data of the Principal Component Analysis to spectral region selected by step (4);
(6) to step (5) the data obtained, to model number of principal components it is 10, is established using techniques of discriminant analysis and verify human body
Sex Discrimination model, with calibration set right judging rate and verifying collection right judging rate evaluation model performance;
(7) saliva sample for taking unknown gender human body acquires NIR transmittance spectroscopy according to step (2) the method, presses
The multi-step processing of spectrum is carried out according to step (3)~(5) the method, then applying step (6) model built carries out human body
Sex Discrimination.
The beneficial effects of the present invention are: the present invention is based on the near infrared spectrums of saliva to sentence in conjunction with chemometric techniques
The gender of other mammal has the advantages that analysis method is accurate and easy to use, tested body compliance is good.
Detailed description of the invention
Fig. 1 is the near-infrared transmission primary light spectrogram of 5 human body saliva samples.
Fig. 2 for the built human body Sex Discrimination model of the present invention differentiation figure.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawing to of the invention preferred
Embodiment is described in detail.
Embodiment 1 is based on saliva and differentiates human body gender
1. the collection and processing of saliva sample
Collect respectively 2 males and 3 women from totally 5 trial volunteer's mornings under rest state active secretion saliva sample
Product number one by one and record gender information.Saliva sample is centrifuged 10min with 3000rpm.
2. the acquisition of NIR transmittance spectroscopy
Instrument: Antaris II ft-nir spectrometer (Thermo company, the U.S.) is furnished with integrating sphere accessory,
Signal acquisition software is Result 3.0.
Spectral measurement condition: resolution ratio 8cm-1, scanning times are 64 times, and scanning range is 10000~4000cm-1。
Spectral measurement method: taking the saliva sample of same number appropriate, is placed in the cuvette of a clean dried, upper
State the NIR transmittance spectroscopy of spectral measurement condition measurement saliva sample.Each sample repeats to load and measure 6 times.Each sample
Identical measuring condition scanning and background correction are all made of before measurement.
30 near-infrared transmission original spectrums of 5 human body saliva samples are as shown in Figure 1.
3. modeling the selection of spectral region
Original spectral data not after pretreatment, the modeling spectral region chosen automatically through 8.0 software of TQ Analyst
For 9881~4119cm-1。
4. the dimensionality reduction of spectroscopic data
Using 8.0 software of TQ Analyst, using Principal Component Analysis selected modeling spectral region 9881~
4119cm-1It is interior that dimensionality reduction is carried out to pretreated spectroscopic data, when selected modeling number of principal components is 10, add up variance contribution ratio
It is 99.8%, and the right judging rate of the calibration set of model built and verifying collection is 100.0%.
5. the foundation and verifying of discrimination model
Using the saliva sample of collected 1 male and 2 women totally 3 trial volunteers as calibration set sample,
Remaining 1 male and 1 women totally 2 trial volunteers saliva sample as verifying collection sample.The above calibration set is used respectively
Sample and verifying collect sample, and to model number of principal components as 10, the Sex Discrimination mould of human body is established and verified using techniques of discriminant analysis
Type.As a result as shown in Fig. 2, the calibration set right judging rate of built discrimination model is 100.0%, verifying collection right judging rate is 100.0%, table
Bright model built has good differentiation performance, is able to use saliva sample and accurately differentiates human body gender.
6. the analysis of unknown gender human saliva sample
Unknown 1, gender human saliva sample is taken, it is saturating according to above-mentioned same procedure processing sample, the near-infrared of acquisition sample
It penetrates spectrum, multi-step processing is carried out to spectrum, the human body Sex Discrimination of the saliva sample is then carried out using model built, as a result
Correctly show it for women.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although passing through ginseng
According to the preferred embodiment of the present invention, invention has been described, however, those skilled in the art should understand that, can be in shape
Various changes are made in formula and to it in details, without departing from protection of the invention defined by the appended claims
Range.
Claims (1)
1. differentiating the other near-infrared spectral analytical method of mammalian based on saliva, which comprises the following steps:
(1) saliva sample of male body and the saliva sample of female body are collected;
(2) resolution ratio that near infrared spectrometer is arranged is 8cm-1, scanning times be 64 times, in 10000~4000cm-1Scanning range
The NIR transmittance spectroscopy of saliva sample obtained by interior acquisition step (1) respectively;
(3) to spectrum obtained by step (2) without pretreatment;
(4) 9881~4119cm is selected in the spectroscopic data obtained by step (3)-1To model spectral region;
(5) dimensionality reduction is carried out using data of the Principal Component Analysis to spectral region selected by step (4);
(6) to step (5) the data obtained, to model the property that number of principal components is 10, is established using techniques of discriminant analysis and verify human body
Other discrimination model, with the performance of calibration set right judging rate and verifying collection right judging rate evaluation model;
(7) saliva sample for taking unknown gender human body acquires NIR transmittance spectroscopy according to step (2) the method, according to step
Suddenly (3)~(5) the method carries out the multi-step processing of spectrum, and then applying step (6) model built carries out the gender of human body
Differentiate.
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CN102145335B (en) * | 2011-03-31 | 2013-04-17 | 浙江省农业科学院 | Automatic photoelectric separator for female and male silkworm eggs |
CN103472008B (en) * | 2013-08-30 | 2016-06-08 | 黑龙江八一农垦大学 | Embryo Gallus domesticus gender identification method in hatching early stage near-infrared hatching egg |
CN104316473A (en) * | 2014-10-28 | 2015-01-28 | 南京农业大学 | Gender determination method for chicken hatching egg incubation early embryo based on hyperspectral image |
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Non-Patent Citations (6)
Title |
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Metabolic profiling of gender: Headspace-SPME/GC–MS and 1H NMR analysis of urine;Shucha Zhang et al.;《Metabolomics》;20110607;第323-334页 * |
Metabolic profiling using principal component analysis, discriminant partial least squares, and genetic algorithms;Z. Ramadan et al.;《Talanta》;20050919;第1683-1691页 * |
Noninvasive biochemical monitoring of physiological stress by Fourier transform infrared saliva spectroscopy;Svetlana Khaustova et al.;《Analyst》;20101231;第3183-3192页 * |
Study on GenderDifferenceBased on Metabolites in Urine by U ltra H igh Performance L iquid Chromatography / T ime of FlightMass Spectrometry;LU Guo et al.;《Chinese Journa l of Chromatography》;20060328;第24卷(第2期);第109-113页 * |
Understanding the human salivary metabolome;I. TAKEDA ET AL.;《NMR Biomed》;20090303;第577-584页 * |
代谢组学技术及其在中西医结合研究中的应用展望;吴斌等;《中西医结合学报》;20070731;第5卷(第4期);第475-478 * |
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