CN107677626A - A kind of portable Tea Polyphenols detecting instrument and detection method - Google Patents
A kind of portable Tea Polyphenols detecting instrument and detection method Download PDFInfo
- Publication number
- CN107677626A CN107677626A CN201711107098.0A CN201711107098A CN107677626A CN 107677626 A CN107677626 A CN 107677626A CN 201711107098 A CN201711107098 A CN 201711107098A CN 107677626 A CN107677626 A CN 107677626A
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- Prior art keywords
- light source
- tea polyphenols
- module
- ultraviolet
- instrument
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- 238000001514 detection method Methods 0.000 title claims abstract description 37
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 33
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 33
- 238000012360 testing method Methods 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- 239000002002 slurry Substances 0.000 claims description 10
- 239000006228 supernatant Substances 0.000 claims description 7
- 238000010521 absorption reaction Methods 0.000 claims description 2
- 241001122767 Theaceae Species 0.000 claims 1
- 238000004088 simulation Methods 0.000 claims 1
- 239000000523 sample Substances 0.000 abstract description 16
- 238000002835 absorbance Methods 0.000 abstract description 9
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 abstract description 5
- 239000012496 blank sample Substances 0.000 abstract description 5
- 230000003287 optical effect Effects 0.000 abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 3
- 230000000007 visual effect Effects 0.000 abstract description 3
- 238000002798 spectrophotometry method Methods 0.000 abstract description 2
- 238000004140 cleaning Methods 0.000 abstract 1
- 235000013616 tea Nutrition 0.000 description 30
- 244000269722 Thea sinensis Species 0.000 description 15
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 6
- 240000003553 Leptospermum scoparium Species 0.000 description 6
- 235000015459 Lycium barbarum Nutrition 0.000 description 6
- 235000009569 green tea Nutrition 0.000 description 6
- 239000000243 solution Substances 0.000 description 5
- 235000006468 Thea sinensis Nutrition 0.000 description 4
- 235000020279 black tea Nutrition 0.000 description 4
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000010561 standard procedure Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000003750 conditioning effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229940074391 gallic acid Drugs 0.000 description 2
- 235000004515 gallic acid Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- 238000004497 NIR spectroscopy Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000001479 atomic absorption spectroscopy Methods 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
A kind of portable Tea Polyphenols detecting instrument, including closed chamber and detection module；It is followed successively by inside closed chamber：Light source, sample cell, for detect the Photoelectric Detection module of ultraviolet light and visual intensity, for control module that is that the analog signal of the output of Photoelectric Detection module is converted into data signal and being resolved and controlled light source module to work to detection signal, for showing the display module of Tea Polyphenols concentration；The light source includes the ultraviolet source for producing 290nm and the visible light source LD for producing 650nm.Cleaning Principle is ultraviolet spectrophotometry, is compared with GB/T8313, it is not necessary to adds forint phenol reagent, it is not necessary to which blank sample contrasts, and using the Tea Polyphenols of various concentrations to the difference of the ultraviolet optical absorbance of specific wavelength, realizes the quick detection to Tea Polyphenols concentration.
Description
Technical field
The invention belongs to technical field of biological, and in particular to a kind of method that Tea Polyphenols is detected with instrument.
Background technology
In order to select the tea tree improved seeds for being adapted to prepare black tea, it is necessary on the basis of field test, sieved using side
The method that choosing, Bian Kuofan, side carry out regional testing and natomical leaf structure identification, selects the excellent tea tree product for fitting black tea processed
Kind, and phenological period, yield, quality and resistance to being selected in type strain synchronize identification, the breed breeding cycle is 10 left
Right year.Therefore, in field test, it is necessary to know the content of Tea Polyphenols in tea tree tender leaf.The total quantity measuring method of Tea Polyphenols is main
Including AAS, near infrared spectroscopy, electrochemical methods etc..GB/T8313《Tea Polyphenols in Tea and catechin contain
The detection method of amount》For forint phenol colorimetric method.These methods are carried out in laboratory.A kind of portable instrumentation is needed,
Can in field quick detection fresh tea leaf Tea Polyphenols content, help tea tree breed quickly to screen, realize that tea tree breed is quick
Seed selection.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of rapid detection apparatus of portable Tea Polyphenols, and with should
The method of instrument quick detection Tea Polyphenols.
In order to solve the technical problem, the present invention devises a kind of instrument of portable quick detection polyphenol content, examines
The principle of survey method uses ultraviolet spectrophotometry, is compared with GB/T8313, is not required to add forint phenol reagent, it is not necessary to blank sample
Contrast, using the Tea Polyphenols of various concentrations to the difference of the ultraviolet optical absorbance of specific wavelength, realize to the fast of Tea Polyphenols concentration
Speed detection.
Whole instrument is made up of the part such as light source module, sample cell, Photoelectric Detection module, microcontroller, display module.
The light source module includes ultraviolet source module and visible light source module；The ultraviolet source module produces specific
The ultraviolet light of 290nm wavelength, for detecting the absorbance of Tea Polyphenols；The visible light source module is that LD contrasts visible optical module,
650nm visible light sources are produced, for substituting blank sample in GB/T8313 methods, eliminate ambient interferences；
The sample cell places tealeaves slurries supernatant to be tested；
The Photoelectric Detection module model LSGPD-UV3.2, is made up of, photodetection photodetection pipe and signal conditioning circuit
Pipe converts optical signals to electric signal, and be amplified by signal conditioning circuit, filter after be converted into standard analog electric signal；
Visual intensity is contrasted for detecting ultraviolet ray intensity and LD；
The microcontroller model STM32ZET6, built-in 12 SAR ADCs, the mould that Photoelectric Detection module is exported
Intend electric signal and be converted to data signal, data signal is handled, calculated and further controls display module；
The OLED of display module model 0.96 ', the data of test, analysis are shown.
GB/T8313 National Standard Methods measure a sample and taken around 1.5 hours, and it is a few minutes that this method, which needs the time, greatly
Detection time is shortened greatly, the quick measure that can be applicable in tea product production process.
Using the portable instrument, the step of detecting Tea Polyphenols in Tea, is as follows：
Step 1：Tea Samples pre-process
（1）Tealeaves cleans；
（2）Tealeaves is made by slurries by beater, stood；
Step 2：Instrument is opened
Switch on power, this instrument, as power supply, is opened 290nm ultraviolet source and 650nm visible light source, incited somebody to action using battery
Instrument returns to zero；
Step 3：The detection of testing sample
（1）The supernatant of tealeaves slurries is taken to be put into sample cell；
（2）Sample cell is put into instrument；
（3）Tealeaves slurries supernatant is tested；
（4）Show Tea Polyphenols in Tea test result.
The present invention has the following advantages that compared with prior art：
1 compares with national standard, and the present invention need not add forint phenol reagent, changes Detection wavelength；Utilize the tea of various concentrations
More phenol solutions are different to the absorbance of 290nm ultraviolet lights, and 650nm visible rays are not absorbed, and pass through the ultraviolet of Tea Polyphenols solution
The absorbance of light subtracts the absorbance of visible ray, reaches the purpose for eliminating ambient interferences, substitutes the effect of blank sample in National Standard Method.
National Standard Method measures a sample and taken around 1.5 hours, and this method time is a few minutes, substantially reduces detection time, can
Apply the quick measure of the polyphenol content in tea tree breed quick breeding and tea products production process.
2nd, this patent realizes that Tea Polyphenols rapid and handy detects, and instrument can industrialized production.
Brief description of the drawings
Fig. 1 instrument composition schematic diagrams
No. 6 Fig. 2 Tea Polyphenols, gallic acid, black tea, green tea 1, green tea 3, green tea sample all band scanning figures.
Embodiment
With reference to embodiment, the present invention is described in detail.The embodiment is only the preferable reality to the present invention
Mode is applied, not makees any formal limitation to the present invention, every technical spirit according to the present invention is implemented to more than
The simple modification that mode is made, equivalent variations and modification, are belonged in the range of technical solution of the present invention.
Fig. 1 is instrument composition schematic diagram, including closed chamber and detection module, is followed successively by inside the closed chamber：Light source
（1）, sample cell（2）, Photoelectric Detection module（3）, control module（4）, display module（5）.Light source（1）Including ultraviolet source and
Visible light source LD；Ultraviolet source produces 290nm ultraviolet lights, it is seen that radiant LD produces 650nm visible rays.Photoelectric Detection mould
Block（3）Model LSGPD-UV3.2 is used for detecting ultraviolet light and visual intensity；Control module（4）Model STM32ZET6,
The analog signal of the output of Photoelectric Detection module, is converted into data signal, microcontroller exists by built-in 12 SAR ADCs
The work of light source module is resolved and controlled to detection signal；Display module（5）For the OLED of model 0.96 ', by detection, resolve
As a result shown.
The Tea Polyphenols solution of various concentrations is different to the absorbance of 290nm ultraviolet light, and 650nm visible ray is not inhaled
Receive, the absorbance of 650nm visible rays is subtracted by the absorbance of the 290nm of Tea Polyphenols solution ultraviolet light, reaches elimination background
The purpose of interference.When Tea Polyphenols detects, 290nm ultraviolet source and 650nm visible light source are opened, while irradiate Tea Polyphenols sample
Product, the light intensity by the 290nm ultraviolet lights of Tea Polyphenols sample and 650nm visible ray is detected, by resolving, eliminate background and do
Disturb, acted on for substituting blank sample in GB/T8313；It is as follows using the specific detecting step of the instrument：
Step 1：Fresh tea leaf sample treatment
1）Tealeaves cleans；
2）Tealeaves is made by slurries by beater, stood；
Step 2：Instrument is opened
Switch on power, while open light source（1）In 290nm ultraviolet sources and 650nm visible light sources；Instrument returns to zero, and shows mould
Block（5）For 0；
Step 3：The detection of testing sample
1）The supernatant of tealeaves slurries is taken to be put into sample cell（2）In；
2）By sample cell（2）It is put into instrument；
3）UV absorption test is carried out to the supernatant of tealeaves slurries；By Photoelectric Detection module（3）Detect ultraviolet light and visible
Luminous intensity；Control module（4）Photoelectric Detection module（3）The analog signal of output be converted into data signal, to detection signal
Resolved and controlled light source module（3）Work；
4）Display module（5）Show Tea Polyphenols test result；
Tea Polyphenols, gallic acid are made into the aqueous solution that concentration is 0.05 mg/ml, by black tea, green tea 1, green tea 3, green tea
No. 6 samples with National Standard Method GB/T8313 detect after, be made into respectively 0.100mg/ml, 0.500mg/ml, 1.000mg/ml, 2./
00 mg/ml concentration, is then scanned with all band respectively, and scanning result is shown in Fig. 2.Display module（5）The results are shown in Table 1.
Claims (2)
- A kind of 1. portable Tea Polyphenols detecting instrument, it is characterised in that：Formed including closed chamber and detection module；The closed chamber Inside is followed successively by：Light source（1）, sample cell（2）, model LSGPD-UV3.2 Photoelectric Detection module（3）, model STM32ZET6 control module（4）, the OLED display module of model 0.96 '（5）；The light source（1）Including producing 290nm's Ultraviolet source and the visible light source LD for producing 650nm；The Photoelectric Detection module（3）For detecting ultraviolet light and visible ray Intensity；The control module（4）Built-in 12 SAR ADCs, for Photoelectric Detection module（3）Output simulation letter Number it is converted into data signal, for being resolved to detection signal, control light source（1）And display module（5）.
- 2. with the method for the detecting instrument detection Tea Polyphenols described in claim 1, it is characterised in that：Comprise the following steps：Step 1：Fresh tea leaf sample treatment1）Tealeaves cleans；2）Tealeaves is made by slurries by beater, stood；Step 2：Open instrumentSwitch on power, while open light source（1）In 290nm ultraviolet sources and 650nm visible light sources；Instrument returns to zero；Step 3：The detection of testing sample1）The supernatant of tealeaves slurries is taken to be put into sample cell（2）In；2）By sample cell（2）It is put into instrument；3）UV absorption test is carried out to the supernatant of tealeaves slurries；4）Display module（5）Show Tea Polyphenols test result.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711107098.0A CN107677626A (en) | 2017-11-10 | 2017-11-10 | A kind of portable Tea Polyphenols detecting instrument and detection method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711107098.0A CN107677626A (en) | 2017-11-10 | 2017-11-10 | A kind of portable Tea Polyphenols detecting instrument and detection method |
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| CN107677626A true CN107677626A (en) | 2018-02-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711107098.0A Pending CN107677626A (en) | 2017-11-10 | 2017-11-10 | A kind of portable Tea Polyphenols detecting instrument and detection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110161025A (en) * | 2019-06-12 | 2019-08-23 | 郑州轻工业学院 | A kind of Continuous Flow Analysis method measuring tea polyphenols in tealeaves and tea product |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1811391A (en) * | 2006-02-22 | 2006-08-02 | 中国农业大学 | Quantitative detecting method for the polyphenol content |
| CN101059426A (en) * | 2007-05-29 | 2007-10-24 | 浙江大学 | Method for non-destructive measurement for tea polyphenol content of tea based on near infrared spectrum technology |
| CN104359879A (en) * | 2014-11-05 | 2015-02-18 | 广西师范大学 | Resonance Rayleigh scattering spectra method measuring tea polyphenol |
-
2017
- 2017-11-10 CN CN201711107098.0A patent/CN107677626A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1811391A (en) * | 2006-02-22 | 2006-08-02 | 中国农业大学 | Quantitative detecting method for the polyphenol content |
| CN101059426A (en) * | 2007-05-29 | 2007-10-24 | 浙江大学 | Method for non-destructive measurement for tea polyphenol content of tea based on near infrared spectrum technology |
| CN104359879A (en) * | 2014-11-05 | 2015-02-18 | 广西师范大学 | Resonance Rayleigh scattering spectra method measuring tea polyphenol |
Non-Patent Citations (2)
| Title |
|---|
| 朱丹: "利用光学-电化学技术快速测定茶多酚含量的方法和装置的研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 韩立 等: "《仪器分析》", 31 October 2014, 吉林大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110161025A (en) * | 2019-06-12 | 2019-08-23 | 郑州轻工业学院 | A kind of Continuous Flow Analysis method measuring tea polyphenols in tealeaves and tea product |
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Application publication date: 20180209 |