CN107974439A - Compounded enzyme preparate, preparation method and its application in tapioca production - Google Patents
Compounded enzyme preparate, preparation method and its application in tapioca production Download PDFInfo
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- 102000004190 Enzymes Human genes 0.000 title claims abstract description 110
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 110
- 240000003183 Manihot esculenta Species 0.000 title claims abstract description 85
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 title claims abstract description 85
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 50
- 238000002360 preparation method Methods 0.000 title claims abstract description 37
- 229940088598 enzyme Drugs 0.000 claims abstract description 109
- 239000002994 raw material Substances 0.000 claims abstract description 24
- 108091005804 Peptidases Proteins 0.000 claims abstract description 16
- 239000004365 Protease Substances 0.000 claims abstract description 16
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 16
- 108010059892 Cellulase Proteins 0.000 claims abstract description 15
- 229940106157 cellulase Drugs 0.000 claims abstract description 15
- 239000003381 stabilizer Substances 0.000 claims abstract description 10
- 230000000694 effects Effects 0.000 claims description 32
- 229920002472 Starch Polymers 0.000 claims description 29
- 239000008107 starch Substances 0.000 claims description 29
- 235000019698 starch Nutrition 0.000 claims description 29
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 20
- 101710130006 Beta-glucanase Proteins 0.000 claims description 15
- 229920002261 Corn starch Polymers 0.000 claims description 11
- 239000008120 corn starch Substances 0.000 claims description 11
- 229940099112 cornstarch Drugs 0.000 claims description 11
- 239000011780 sodium chloride Substances 0.000 claims description 10
- 239000000654 additive Substances 0.000 claims description 7
- 230000000996 additive effect Effects 0.000 claims description 7
- 239000000049 pigment Substances 0.000 claims description 7
- 238000000926 separation method Methods 0.000 claims description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 6
- 239000000835 fiber Substances 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 6
- 239000008187 granular material Substances 0.000 claims description 5
- 239000002023 wood Substances 0.000 claims description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 3
- 229920002774 Maltodextrin Polymers 0.000 claims description 3
- 239000005913 Maltodextrin Substances 0.000 claims description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 3
- 235000011187 glycerol Nutrition 0.000 claims description 3
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical group O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 3
- 229940035034 maltodextrin Drugs 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 229920000858 Cyclodextrin Polymers 0.000 claims description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 2
- 239000001116 FEMA 4028 Substances 0.000 claims description 2
- 229930195725 Mannitol Natural products 0.000 claims description 2
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims description 2
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims description 2
- 229960004853 betadex Drugs 0.000 claims description 2
- 239000000594 mannitol Substances 0.000 claims description 2
- 235000010355 mannitol Nutrition 0.000 claims description 2
- 229940032147 starch Drugs 0.000 claims description 2
- 244000061456 Solanum tuberosum Species 0.000 claims 1
- 235000002595 Solanum tuberosum Nutrition 0.000 claims 1
- 238000013329 compounding Methods 0.000 claims 1
- 238000005265 energy consumption Methods 0.000 abstract description 8
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 abstract description 2
- 229920002498 Beta-glucan Polymers 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 26
- 230000008569 process Effects 0.000 description 12
- 239000002699 waste material Substances 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 230000008901 benefit Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- -1 by mass percentage Substances 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000010924 continuous production Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 229920000945 Amylopectin Polymers 0.000 description 1
- 108010001682 Dextranase Proteins 0.000 description 1
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 229920000881 Modified starch Polymers 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 108010093305 exopolygalacturonase Proteins 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 235000019426 modified starch Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920001592 potato starch Polymers 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C12Y402/02002—Pectate lyase (4.2.2.2)
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Abstract
A kind of compounded enzyme preparate, preparation method and its application in tapioca production, are related to enzymic preparation field.The compounded enzyme preparate of the embodiment of the present invention is blended by following raw material, and by mass percentage, raw material includes：20%~40% cellulase；5%~20% beta glucan enzyme；5%~20% zytase；10%~30% pectase；5%~20% protease；0%~10% stabilizer；0%~20% carrier, the raw material of compounded enzyme preparate are easy to get, and preparation method is simple, are exclusively used in tapioca production；Application of the compounded enzyme preparate of the embodiment of the present invention in tapioca production, improves the yield and quality of tapioca, reduces the viscosity and moisture of residue, reduces the energy consumption of production process, reduce production cost.
Description
Technical field
The present invention relates to enzymic preparation field, and more particularly to a kind of compounded enzyme preparate, preparation method and its in tapioca
Application in production.
Background technology
With the continuous maturation of various deep process technologies, it is high that cassava has been increasingly becoming universally acknowledged comprehensive utilization value
Industrial crops and the important raw material of industry.Tapioca silty is fine and smooth, few containing impurity such as protein, and amylopectin content ratio
Cornstarch, wheaten starch are all high.In addition, the hydroxy chemical property in tapioca molecular structure is active, especially suitable for life
Produce the starch derivatives of various uses.These characteristics of tapioca, make it in food, papermaking, weaving, papermaking, medicine, change
The industries such as work, casting, oil, agricultural are widely used.
New fresh cassava is mainly made of the other materials such as starch and cellulose, a small amount of protein, inorganic salts, tapioca
Production process is actually a physical separating process, and root Ju starch is more than the characteristic of water insoluble in cold water and proportion, by big
The water and special mechanical equipment of amount, starch is separated from the suspension of water, so as to achieve the purpose that to recycle starch.Tradition
Tapioca production process in can produce substantial amounts of waste water and waste residue, there is statistics to show that every production 1t tapiocas can at least produce
Waste residue, the waste water of 10~15t of raw about 1.0~1.5t.Researcher has found still to contain in waste residue in the component analysis to waste residue
There is much starch (butt, 50%~60%), in tapioca production process, partial starch fails to extract this explanation,
This is undoubtedly a no small loss in tapioca price increasingly high today to tapioca manufacturers.
Traditional tapioca production technology is to produce tapioca by raw material of new fresh cassava, using vertical sieve sieving technology
Tapioca is screened out to come, this technique falls behind, and energy consumption is big, and starch extraction rate is relatively low, and product quality is also not sufficiently stable, thus
Tapioca production technology level is improved, is had great importance to tapioca production.Have some at present by improving wood
Sweet potato starch manufacturing machine equipment and technique reach the method for above-mentioned purpose, but the production cost of these methods is still higher, and
And the yield and quality of tapioca still have much room for improvement.
Therefore, the cost that tapioca produces need to be reduced with a kind of new method, improves the yield and product of tapioca
Matter.
The content of the invention
It is an object of the invention to provide a kind of compounded enzyme preparate and preparation method thereof, raw material is easy to get, and method is simple, specially
In tapioca production.
Another object of the present invention is to provide a kind of application of compounded enzyme preparate in tapioca production, cassava is improved
The yield and quality of starch, reduce the viscosity and moisture of residue, reduce the energy consumption of production process, reduce production cost.
The present invention is solved its technical problem and is realized using following technical scheme.
The present invention proposes a kind of compounded enzyme preparate, it is blended by following raw material, by mass percentage, raw material bag
Include：
Further, in present pre-ferred embodiments, the appropriate effect temperature of compounded enzyme preparate is 20~70 DEG C, suitably
PH is 3.0~7.0.
Further, in present pre-ferred embodiments, stabilizer is included at least selected from sodium chloride, glycerine, trehalose, sweet
Reveal at least one of group of alcohol, sorbierite, inositol composition.
Further, in present pre-ferred embodiments, carrier is included at least selected from cornstarch, wheaten starch, cassava
At least one of group that starch, farina, green starch, beta-cyclodextrin, maltodextrin, converted starch form.
Further, in present pre-ferred embodiments, enzyme activity >=1.2 × 10 of cellulase5U/g, beta glucan
Enzyme activity >=0.5 × 10 of enzyme5U/g, enzyme activity >=1 × 10 of zytase5U/g, enzyme activity >=0.3 × 10 of pectase5U/
G, enzyme activity >=1 × 10 of protease5U/g。
A kind of preparation method of above-mentioned compounded enzyme preparate, by mass percentage, weighs raw material, physical mixed is uniform.
Further, in present pre-ferred embodiments, compounded enzyme preparate is by the way of coating or immobilization.
A kind of application of above-mentioned compounded enzyme preparate in tapioca production, adds multiple in tapioca production process
With enzyme preparation, the starch granules for fiber to be wrapped up discharges.
Further, in present pre-ferred embodiments, the additive amount of compounded enzyme preparate for cassava weight 0.01%~
0.1%.
Further, in present pre-ferred embodiments, add pigment broken cassava and/or screenings separation process in add
Compounded enzyme preparate.
Compounded enzyme preparate, preparation method and its beneficial effect of the application in tapioca production of the embodiment of the present invention
It is：The compounded enzyme preparate of the embodiment of the present invention is blended by following raw material, and by mass percentage, raw material includes：20%
~40% cellulase；5%~20% 1,4 beta-glucanase；5%~20% zytase；10%~30% pectase；5%~
20% protease；0%~10% stabilizer；And 0%~20% carrier, the raw material of compounded enzyme preparate are easy to get, preparation method letter
It is single, it is exclusively used in tapioca production；Application of the compounded enzyme preparate of the embodiment of the present invention in tapioca production, is improved
The yield and quality of tapioca, reduce the viscosity and moisture of residue, reduce the energy consumption of production process, reduce production cost.
Embodiment
, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer, is the conventional production that can be obtained by commercially available purchase
Product.
The compounded enzyme preparate of the embodiment of the present invention, preparation method and its application in tapioca production are carried out below
Illustrate.
The embodiment of the present invention provides a kind of compounded enzyme preparate, it is blended by following raw material, by mass percentage,
Raw material includes：20%~40% cellulase；5%~20% 1,4 beta-glucanase；5%~20% zytase；10%~30%
Pectase；5%~20% protease；0%~10% stabilizer；And 0%~20% carrier.Raw material preferably includes：30% is fine
The plain enzyme of dimension；10% 1,4 beta-glucanase；10% zytase；20% pectase；10% protease；5% stabilizer；And 15% carry
Body.The appropriate effect temperature of the compounded enzyme preparate is 20~70 DEG C, and appropriate pH is 3.0~7.0.
Wherein, enzyme activity >=1.2 × 10 of cellulase5U/g, enzyme activity >=0.5 × 10 of 1,4 beta-glucanase5U/g, wood
Enzyme activity >=1 × 10 of dextranase5U/g, enzyme activity >=0.3 × 10 of pectase5U/g, enzyme activity >=1 × 10 of protease5U/
g。
Stabilizer is included at least in the group that sodium chloride, glycerine, trehalose, mannitol, sorbierite, inositol form extremely
Few one kind, but these stabilizers are not limited to, the preferred sodium chloride of compounded enzyme preparate of the present embodiment is as stabilizer.
Carrier is included at least selected from cornstarch, wheaten starch, tapioca, farina, green starch, β-ring paste
At least one of group that essence, maltodextrin, converted starch form.But it is not limited to these starch and its derivative, this implementation
The preferred cornstarch of compounded enzyme preparate of example is carrier.
The embodiment of the present invention provides a kind of preparation method of above-mentioned compounded enzyme preparate, by mass percentage, weighs original
Material, physical mixed are uniform；It is lost in reduce enzyme, compounded enzyme preparate can be obtained by the way of coating or immobilization, compounded
The formulation of enzyme preparation can be liquid, powder or particle.Preferably using conventional spray dried form, what is obtained answers the present embodiment
It is powder formulation with enzyme preparation.
The embodiment of the present invention provides a kind of application of above-mentioned compounded enzyme preparate in tapioca production, and new fresh cassava contains
There is the component such as much starch and cellulose, lignin and a small amount of protein, wherein, starch granules is by the cellulose of internal layer, wood
Quality and the winding of the pectin of a small amount of protein and outer layer are wrapped in.In order to make more starch granules be released from fiber
Come, it is necessary to which the structure for being wound parcel interrupts.The present embodiment adds compounded enzyme preparate in tapioca production process, is used for
The starch granules that fiber wraps up is discharged, the yield and quality of tapioca can be improved, reduce the viscosity and moisture of residue,
The energy consumption of production process is reduced, reduces production cost.
Due to carrying out enzyme treatment process using different enzyme preparations, Different Effects can be produced to tapioca extracting rate, only adopted
With the compounded enzyme preparate of the present embodiment, the raising effect and economy to tapioca yield are optimal.
The present embodiment specifically add pigment broken cassava and/or screenings separation process in add compounded enzyme preparate, but not office
It is limited to adding pigment broken and carrying out enzyme treatment process in screenings separation process, can be also carried out in other process.
Due to also strengthening therewith with the effect of the increase of enzyme preparation usage amount, enzyme preparation, but when additive amount is 0.1%
When, the lifting of tapioca yield is substantially slowed down, while in view of the economy of production and application, the use of compounded enzyme preparate
Amount is no more than 0.1% and is advisable, and the additive amount of compounded enzyme preparate is specially the 0.01%~0.1% of cassava weight.
Those skilled in the art should be understood：The specific additive amount and occupation mode of the compounded enzyme preparate of the present embodiment with
Effect that the ability of used equipment, condition, needs reach and reach effect time it is related.In tapioca production process
The middle compounded enzyme preparate for adding the present embodiment, has the following advantages：
1st, tapioca yield is improved using biological technique method first, rather than is reached by way of improving mechanical equipment
Into effect recycles this kind of indirect mode better than the processing to waste residue.
2nd, the yield of tapioca can be not only improved, the quality of tapioca can also be improved：In compounded enzyme preparate
Under effect, the impurity partial hydrolysis such as a small amount of fiber and polysaccharide for containing in tapioca is soluble component, so as to improve cassava
The purity of starch, improves its quality.
3rd, add pigment broken and screenings separation process in add enzyme treatment process, using original starch-making equipment, be not required to
Increase new equipment and energy consumption, it is only necessary to certain enzyme preparation action time, you can improve the separative efficiency of tapioca, improve
The yield of tapioca, has direct economic benefit.
4th, the separation of waste residue and waste water is contributed to, one side waste residue water content reduces, and reduces drying quantity of steam, saves
Energy consumption, on the other hand, can obtain more recycle-waters, be conducive to save water resource and environmental protection, have a great society
Can benefit.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
The present embodiment provides a kind of compounded enzyme preparate for tapioca production, it uses following preparation method to be made：
Raw material is weighed by mass percentage：Cellulase 20%, 1,4 beta-glucanase 20%, zytase 20%, pectase
20%th, protease 20%, above enzyme preparation (cellulase, 1,4 beta-glucanase, zytase, pectase, protease) is force
The enzyme preparation product of Biological Co., Ltd. is raised by Chinese Xinhua.Wherein, cellulase activity about 120000U/g, examination criteria
QB2583-2005；Activity of beta-glucanase about 50000U/g, examination criteria QB/T4481-2013；Xylanase activity is about
100000U/g, examination criteria QB/T4483-2013；Pectinase activity about 30000U/g, examination criteria GB1886.17-2016；
Prolease activity about 100000U/g, examination criteria GB1886.17-2016.
It is during preparation that above-mentioned each raw material components physical mixed is uniform, that is, obtain compounded enzyme preparate.
Embodiment 2
The present embodiment provides a kind of compounded enzyme preparate for tapioca production, in its preparation method and embodiment 1
Preparation method is roughly the same, and difference is：
In the present embodiment, raw material is weighed by mass percentage：Cellulase 20%, 1,4 beta-glucanase 10%, zytase
10%th, pectase 20%, protease 10%, sodium chloride 10%, cornstarch 20% form, and finally obtain compounded enzyme preparate.
Embodiment 3
The present embodiment provides a kind of compounded enzyme preparate for tapioca production, in its preparation method and embodiment 1
Preparation method is roughly the same, and difference is：
In the present embodiment, raw material is weighed by mass percentage：Cellulase 30%, 1,4 beta-glucanase 10%, zytase
10%th, pectase 20%, protease 10%, sodium chloride 5%, cornstarch 15% form, and finally obtain compounded enzyme preparate.
Embodiment 4
The present embodiment provides a kind of compounded enzyme preparate for tapioca production, in its preparation method and embodiment 1
Preparation method is roughly the same, and difference is：
In the present embodiment, raw material is weighed by mass percentage：Cellulase 20%, 1,4 beta-glucanase 10%, zytase
10%th, pectase 30%, protease 10%, sodium chloride 5%, cornstarch 15% form, and finally obtain compounded enzyme preparate.
Embodiment 5
The present embodiment provides a kind of compounded enzyme preparate for tapioca production, in its preparation method and embodiment 1
Preparation method is roughly the same, and difference is：
In the present embodiment, raw material is weighed by mass percentage：Cellulase 30%, 1,4 beta-glucanase 10%, zytase
10%th, pectase 30%, protease 10%, sodium chloride 5%, cornstarch 5% form, and finally obtain compounded enzyme preparate.
The application effect of the compounded enzyme preparate of embodiment is detected below.
First, new fresh cassava is being divided into 6 groups, tapioca is produced according to same process.1 group therein is blank control
Group, remaining 5 groups when adding pigment broken new fresh cassava, every group of correspondence adds a kind of compounded enzyme preparate of embodiment 1~5, different
Compounded enzyme preparate formula it is as shown in table 1, all compounded enzyme preparate additive amounts are the 0.05% of cassava quality, and in reaction pH=
4.0~5.0,40 DEG C of reaction temperature, produces tapioca, final 6 groups of tapioca yield under conditions of reaction time 60min
As shown in table 2.
The formula of 1 compounded enzyme preparate of table
The tapioca yield of the different groups of table 2
As shown in table 2, raising best results of the formula of embodiment 5 to tapioca yield, secondly embodiment 3 is matched somebody with somebody
Side, both gap very littles.Consider economy, embodiment 3 is formulated more worth recommendation, i.e., fiber by mass percentage
Plain enzyme 30%, 1,4 beta-glucanase 10%, zytase 10%, pectase 20%, protease 10%, sodium chloride 5%, cornstarch
The compounded enzyme preparate of 15% composition.
2nd, in the actual production process of tapioca, common process, and the compound enzyme with embodiment 3 are carried out at the same time
Preparation (is calculated in mass percent, its formula is：Cellulase 30%, 1,4 beta-glucanase 10%, zytase 10%, pectase
20%th, protease 10%, sodium chloride 5%, cornstarch 15%), add that pigment are broken and screenings separation process in add it is above-mentioned multiple
Enzyme treatment process is carried out with enzyme preparation, additive amount is the 0.05% of cassava weight, and processing time 30min, temperature, pH are without volume
Outer regulation and control.Common process of the contrast without using compounded enzyme preparate and the enzyme technique using compounded enzyme preparate were in continuous production 7 days
Data afterwards, it is as shown in the table：
3 common process of table and the enzyme technique continuous production data of 7 days
| Tapioca yield/% | Waste residue water content/% | |
| Common process | 64.5 | 68.2 |
| Enzyme technique | 67.8 | 62.3 |
As shown in table 3, after using enzyme technique, the yield of tapioca is improved to 67.8% by 64.5%, and waste residue is aqueous
Amount is reduced to 62.3% by 68.2%, and the raising of one side starch recoveries directly improves productivity effect, and another aspect waste residue contains
The reduction of water, reduces the drying process cost of waste residue, in addition, the moisture of reduction enters reuse aqueous systems, is conducive to save
Water resource and environmental protection, therefore, the enzyme technique of the present embodiment have important economic benefit and society to tapioca industry
Benefit.
In conclusion compounded enzyme preparate of the embodiment of the present invention and preparation method thereof, raw material is easy to get, and method is simple, specially
In tapioca production；Application of the compounded enzyme preparate in tapioca production, improves the yield and quality of tapioca,
The viscosity and moisture of residue are reduced, reduces the energy consumption of production process, reduces production cost.
Embodiments described above is part of the embodiment of the present invention, instead of all the embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
- A kind of 1. compounded enzyme preparate, it is characterised in that it is blended by following raw material, by mass percentage, the original Material includes：Cellulase 20%~40%；1,4 beta-glucanase 5%~20%；Zytase 5%~20%；Pectase 10%~30%；Protease 5%~20%；Stabilizer 0%~10%；Carrier 0%~20%.
- 2. compounded enzyme preparate according to claim 1, it is characterised in that the appropriate effect temperature of the compounded enzyme preparate is 20~70 DEG C, appropriate pH is 3.0~7.0.
- 3. compounded enzyme preparate according to claim 1, it is characterised in that the stabilizer include at least selected from sodium chloride, At least one of group that glycerine, trehalose, mannitol, sorbierite, inositol form.
- 4. compounded enzyme preparate according to claim 1, it is characterised in that the carrier include at least selected from cornstarch, In the group that wheaten starch, tapioca, farina, green starch, beta-cyclodextrin, maltodextrin, converted starch form It is at least one.
- 5. compounded enzyme preparate according to claim 1, it is characterised in that enzyme activity >=1.2 of the cellulase × 105U/g, enzyme activity >=0.5 × 10 of the 1,4 beta-glucanase5U/g, enzyme activity >=1 × 10 of the zytase5U/g, institute State enzyme activity >=0.3 × 10 of pectase5U/g, enzyme activity >=1 × 10 of the protease5U/g。
- 6. a kind of preparation method of compounded enzyme preparate as claimed in claim 1, it is characterised in that by mass percentage, claim Raw material is taken, physical mixed is uniform.
- 7. the preparation method of compounded enzyme preparate according to claim 1, it is characterised in that the compounded enzyme preparate is using bag The mode of clothing or immobilization.
- 8. application of a kind of compounded enzyme preparate as claimed in claim 1 in tapioca production, it is characterised in that in cassava The compounded enzyme preparate is added in starch production process, the starch granules for fiber to be wrapped up discharges.
- 9. application of the compounded enzyme preparate according to claim 8 in tapioca production, it is characterised in that the compounding The additive amount of enzyme preparation is the 0.01%~0.1% of cassava weight.
- 10. application of the compounded enzyme preparate according to claim 8 in tapioca production, it is characterised in that by wood Potato add pigment broken and/or screenings separation process in add the compounded enzyme preparate.
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| CN112522237A (en) * | 2020-12-22 | 2021-03-19 | 武汉新华扬生物股份有限公司 | Compound enzyme preparation for producing fried dough twist and using method thereof |
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