CN102162198B - Multienzyme complex preparation and preparation method and application thereof - Google Patents
Multienzyme complex preparation and preparation method and application thereof Download PDFInfo
- Publication number
- CN102162198B CN102162198B CN2011100289126A CN201110028912A CN102162198B CN 102162198 B CN102162198 B CN 102162198B CN 2011100289126 A CN2011100289126 A CN 2011100289126A CN 201110028912 A CN201110028912 A CN 201110028912A CN 102162198 B CN102162198 B CN 102162198B
- Authority
- CN
- China
- Prior art keywords
- stabilizing agent
- mixture
- multienzyme
- agricultural wastes
- compound formulation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
The invention provides a multienzyme complex preparation and a preparation method and application thereof. The multienzyme complex preparation is characterized by being prepared by adding water to multienzyme complex, wherein the weight ratio of the multienzyme complex to the water is 1:(50-100); and the multienzyme complex comprises the following components in parts by weight: 30-35 parts of laccase, 25-35 parts of xylanase, 10-20 parts of pectinase, 0.1-10 parts of amylase, 0.3-10 parts of lipase, 0.1-5 parts of tannase, 0.1-3 parts of coenzyme, 0.1-3 parts of stabilizing agent and 0.3-25 parts of diatomite. The multienzyme complex preparation can be applied to the preparation process of the fiber pulp for papermaking, the fiber pulp in accordance with the papermaking production requirement can be obtained without further adopting the high-temperature/pressure chemical cooking method in the pulping process, and the original lignin can be preserved to the greatest extent in the fiber pulp preparing process.
Description
Technical field
The present invention relates to a kind of biologic product, relate in particular to a kind of multienzyme compound formulation.
Background technology
Increasing in recent years biologic product is widely used in the commercial production field; It has significantly reduced the pollution to environment when replacing chemicals to play a role; For example at the paper industry production field; Utilize enzyme preparation preliminary treatment chemical pulping method to replace the pure chemistry pulp-making method, reduced the use amount of chemicals in the pure chemistry pulp-making method dramatically, and pulping process has the sewage emissions of high BOD, COD load and severe toxicity, strong carcinogenic material.
Yet; Existing enzyme preparation preliminary treatment chemical pulping method is after at first using enzyme preparation that material is carried out preliminary treatment; Still need further to adopt the method for high-temperature high-pressure chemical boiling (mainly being alkaline boiling) could obtain to meet the fibre stuff that paper requires, wherein, farthest destroyed at chemical steaming process lignin; Make to be difficult to after the slurrying lignin separated further from waste liquid and utilize, for example: be used for the production of functional biological fertilizer; And the produced slurry of above-mentioned enzyme preparation preliminary treatment chemical pulping method is used to make packing box or paper products; Because of using chemicals in preparation slurry process; Unavoidably contain chemical residual in packing box that it is prepared or the paper products; Exist safe in utilization and degraded after stain problem of environment, therefore need further processing just can address the above problem, therefore cause the raising of the processing cost of paper products such as packing box; Make production technology complicated more, be unfavorable for large-scale production and processing.
How therefore a kind of new enzyme preparation be provided; Making does not need further to adopt the step of high-temperature high-pressure chemical boiling at pulping process; Just can obtain to meet the fibre stuff that paper requires; And when producing fibre stuff, at utmost keep the lignin of ecosystem, and make production process cleaning, energy-conservation, efficiently become problem to be solved arranged.
Summary of the invention
The invention provides a kind of multienzyme compound formulation, it uses the proportioning of specific enzyme preparation, stabilizing agent and coenzyme to make multienzyme compound formulation storage-stable over a period to come, and still has good vigor in use.
The present invention also provides the preparation method of multienzyme compound formulation; Specific addition and interpolation order through different enzyme preparations, stabilizing agent and coenzyme; Make that the multienzyme compound formulation prepare over a period to come can storage-stable, and still have good vigor in use.
The present invention also provides above-mentioned multienzyme compound formulation utilizing agricultural wastes to obtain the application in the fibre stuff; Use this multienzyme compound formulation; The preparation process of whole fibre stuff need not used chemicals, can obtain to meet the fibre stuff that paper requires.
The present invention also provides the method for utilizing said multienzyme compound formulation to obtain fibre stuff from agricultural wastes; Through above-mentioned multienzyme compound formulation being applied to the preparation process of fibre stuff; Need not adopt the method for high-temperature high-pressure chemical boiling at pulping process; Just can obtain to meet the fibre stuff that paper requires, and when producing fibre stuff, can at utmost keep the lignin of ecosystem.
The present invention further also provides a kind of fibre stuff, and said fibre stuff is prepared from according to the method described above; Said slurry can be used for preparation does not have chemical residual, environment friendly and pollution-free packing box or paper products.
Multienzyme compound formulation provided by the invention, it is formulated to add water by multienzyme complex, and wherein, the weight ratio of multienzyme complex and water is 1: 50-100; The prescription of said multienzyme complex comprises the component of following weight portion: the laccase of 30-35 part; The zytase of 25-35 part; The pectase of 10-20 part; 0.1-10 the amylase of part; 0.3-10 the lipase of part; 0.1-5 the tannase of part; 0.1-3 the coenzyme of part; 0.1-3 the stabilizing agent of part; 0.3-25 the diatomite of part; Said stabilizing agent is made up of stabilizing agent A, stabilizing agent B and stabilizing agent C;
Said stabilizing agent A accounts for 30% of stabilizer formula amount, by Ca
2+Form with polyvinylpyrrolidone;
Said stabilizing agent B accounts for 50% of stabilizer formula amount, is made up of EDTA and dithiothreitol (DTT);
Said stabilizing agent C accounts for 20% of stabilizer formula amount, is made up of polyvinylpyrrolidone and EDTA.
In one embodiment of the invention, Ca among the said stabilizing agent A
2+With the weight ratio of polyvinylpyrrolidone be 8-10: 1; The weight ratio of EDTA and dithiothreitol (DTT) is 1 among the said stabilizing agent B: 30-50; The weight ratio of polyvinylpyrrolidone and EDTA is 70-90 among the said stabilizing agent C: 1;
Said coenzyme is made up of in nicotinic acid, folic acid, biotin or the riboflavin one or more.
The preparation method of said multienzyme compound formulation comprises:
1) the pH value with water transfers to 7.5-9, adds amylase, lipase, tannase and stirs adding stabilizing agent A, mixing;
2) in the mixture that step 1) obtains, add after pectase, zytase, laccase stir, add the coenzyme and the stabilizing agent B of formula ratio 60%, mixing left standstill 5-10 minute;
3) in step 2) add coenzyme and the diatomite mixing of formula ratio 20% in the mixture that obtains, place and leave standstill after 5-36 hour packing under the 4-6 ℃ of condition and store;
4) before the multienzyme compound formulation uses 0.5-1 hour, the coenzyme and the stabilizing agent C of adding formula ratio 20% in the mixture that step 3) obtains, mixing.
Ca in the above-mentioned multienzyme compound formulation
2+Stabilizing agent is by any Ca that provides
2+Material provide, for example: CaCl
2, CaSO
4, CaNO
3Deng; Ca in the said multienzyme compound formulation
2+The amount of stabilizing agent is by CaCl
2The amount of calculating is as selecting CaSO
4, CaNO
3Deng, then press with equivalent CaCl
2Conversion Calculation goes out the weight portion that corresponding calcium salt uses.Stabilizing agent A joins existing usefulness at present, leaves standstill after stabilizing agent B prepares after 5-10 minute to use.In the said coenzyme, generally select purity greater than 99.5% nicotinic acid, purity greater than 99% biotin, purity greater than 98% riboflavin and purity greater than 98% folic acid.
Among the preparation method of above-mentioned multienzyme compound formulation; The weight ratio of multienzyme complex and water; And step 1)-3) effect of adding stabilizing agent and coenzyme in is in order to guarantee that the mixture that step 3) obtains can store 20-25 days at ambient temperature; The activity that keeps various enzymes in the multienzyme compound formulation simultaneously; The effect that limits before use (that is, thin up is for before discongesting liquid) 0.5-1 hour interpolation stabilizing agent and coenzyme in the step 4) is in order to guarantee various enzymes activity in use in the multienzyme compound formulation.So; According to the characteristic of various enzymes used in the present invention the kind of coenzyme and consumption and proportion of composing are carried out suitable choice; Those skilled in the art can accomplish based on its rudimentary knowledge; When adopting multiple coenzyme combination to use, the present invention does not do particular determination for concrete portfolio ratio.
Based on above-mentioned multienzyme compound formulation, the present invention further provides above-mentioned multienzyme compound formulation utilizing agricultural wastes to obtain the application in the fibre stuff; And utilize above-mentioned multienzyme compound formulation to obtain the method for fibre stuff from agricultural wastes.Said agricultural wastes are stalk, root, the stem of crops in the agriculture and forestry production process, and fibrous part such as limb is like straw, maize straw, wheat straw, straw, cotton stalk etc.
Provided by the inventionly utilize above-mentioned multienzyme compound formulation to may further comprise the steps from the method that agricultural wastes obtain fibre stuff:
1) use the liquid of discongesting that forms by said multienzyme compound formulation thin up at room temperature agricultural wastes to be carried out preimpregnation and obtain the preimpregnation mixture; Wherein saidly discongest that the weight ratio of multienzyme complex and said agricultural wastes is 0.7-1 in the liquid: 100; And with total restatement of said preimpregnation mixture, the content of said agricultural wastes is 25-30wt%; The pH value of regulating above-mentioned preimpregnation mixture then is to 7.5-11, in not being higher than 65 ℃ temperature range, said preimpregnation mixture carried out at least 6 hours discongesting, and obtains discongesting mixture;
2) add fabric softening and cationic surfactant in the mixture above-mentioned discongesting, the said mixture of discongesting is carried out 30-60 minute softening and slaking, obtain mixture softening and slaking at 60-80 ℃;
3) above-mentioned softening mixture with slaking is ground separate, obtain the fibre stuff of agricultural wastes.
In one embodiment of the invention; Said agricultural wastes for through get the raw materials ready, the agricultural wastes of dedusting removal of impurities and brooming step process; Wherein said material preparation step comprises the process with the agricultural wastes segment, and said brooming step comprises agricultural wastes are processed into thread process.Further, in the brooming agricultural wastes, can spray water to agricultural wastes, making the dry weight of agricultural wastes in the agricultural wastes behind the water spray is 50-60wt%.Above-mentioned melting process can use conventional chaffcutter to carry out; The dedusting removal of impurities can use conventional deduster to carry out; Said brooming can use conventional brooming equipment to carry out; Mill is separated process and also can be carried out once or once according to the purposes of fibre stuff the specification of the paper products that will make (promptly), can make the mill of routine separate equipment and carry out, for example the pulping equipment in the paper technology.
In another embodiment of the present invention; The weight ratio of said fabric softening and said agricultural wastes (being preimpregnation step agricultural wastes before) is 1-2: 100, and the weight ratio of said cationic surfactant and said agricultural wastes (being preimpregnation step agricultural wastes before) is 1-2: 100; The concentration of discongesting the mixture solid content that is used for softening with slaking is 35-40wt%; The concentration that is used for grinding the mixture solid content of the softening and slaking of separating is 15-35wt%.
Said cationic surfactant can be cationic surfactants such as fatty amine salt, ethanolamine salt, polyethylene polyamines salt and quaternary ammonium salt; Said fabric softening can be string softening agents such as imidazoline.
The present invention further also provides a kind of fibre stuff, and said fibre stuff is prepared from according to the method described above.
Provided by the invention from the method for agricultural wastes acquisition fibre stuff; Fully react for making in the multienzyme compound formulation various enzyme components and agricultural wastes discongesting process; And make each enzyme component all by full consumption, need 6 hours at least, in the practice according to the difference of material or enzyme preparation amount; Generally carry out to discongest in 6-72 hour fully; In commercial production, take all factors into consideration other factors such as time and cost, make the process of discongesting carry out to discongest in 6-24 hour fully, can simply through the experiences such as resistance that agricultural wastes change color, feel and firmness change and taperer insert judge to discongest whether accomplish usually; Also can judge the degree of discongesting of raw material through microscopy, also can select other conventional meanses of this area to judge certainly as the case may be.Further, the softening degree with slaking of fiber can judge that the mill degree of separating can be judged through detecting the beating degree method through detection fibers broken resistance intensity method.
The multienzyme compound formulation of specific composition provided by the invention and specific preparation method thereof make this multienzyme compound formulation stable preservation over a period to come; And still has good vigor in use; Concrete enzyme preparation in the said multienzyme compound formulation is and is purchased product, and wherein the enzyme work of laccase is generally 3000U/g, and the enzyme work of zytase is generally 2,000,000 U/g; The enzyme work of pectase is generally 900,000 U/g; Diastatic enzyme work is generally 800,000 U/g, and the enzyme work of lipase is generally 25000U/g, and the enzyme work of tannase is generally 200 tannase units/gram; Scope is different with above-mentioned scope if the enzyme of the enzyme preparation of using is lived; Can convert accordingly and confirm the weight portion that uses in the prescription of multienzyme complex; The method of utilizing above-mentioned multienzyme compound formulation from agricultural wastes acquisition fibre stuff provided by the invention has the following advantages:
(1) liquid of discongesting that adopts the multienzyme compound formulation to be prepared into is discongested the agriculture waste fibres; Avoided the electrochemical conditions of HTHP strong acid-base; Make fiber and lignin realization original position dissociate, rather than farthest destroy lignin structure, make lignin keep than ecosystem; Also reached the enough big degree of polymerization after removing with fiber; Be convenient to adopt conventional physico-chemical process just can the lignin composition thoroughly therefrom flocculated, get into the production of functional biological fertilizer, make production process accomplish cleaning, energy-conservation, efficient in water treatment stage.
(2) make to split between the cell membrane of agricultural wastes and leave through agricultural wastes being carried out brooming; Various enzyme components in the promotion multienzyme compound formulation can get into iuntercellular fast and carry out work; Estranged efficient and speed have been improved greatly; Can the time of discongesting the agricultural wastes structure be reduced to 16 hours even shorter, for large-scale industrial production has been created sufficient condition.The existing technology of producing fibre stuff then needs 30-40 days time could accomplish the degraded of the compositions such as lignin of 60-70%.
(3) because production process has been avoided HTHP strong acid, highly basic condition; And do not use chemicals; Said fibre stuff can be used for suppressing environment-friendly type agricultural production packing material such as cutlery box, rice packaging box, tea caddy, vegetables packing box etc.; When the packing material of producing is degraded after use, can the residual harmful substance contaminated environment yet.
(4) adopt this technology to produce, technology is simple, and the utilization of agricultural wastes material has been accomplished to utilize fully, and except cellulose components was converted into fibre stuff, other compositions transferred the raw material of producing biological organic fertilizer to, and comprehensive benefit is high.In addition, equipment investment is lower more than 1/3rd than tradition, and the production cost of fibre stuff is lower more than 1/3rd than tradition.
The specific embodiment
Further specify the technical scheme of the embodiment of the invention below in conjunction with specific embodiment.Various enzymes, coenzyme and the stabilizing agent that uses in following examples all can obtain through commercial the purchase.
Embodiment 1, utilize multienzyme compound formulation of the present invention to obtain fibre stuff from straw
To the straw of 100kg, the multienzyme compound formulation that present embodiment uses is prepared from for the water that the multienzyme complex 0.7kg adds 35-70kg, and the prescription of said multienzyme complex comprises the component of following weight portion: the laccase of 31-33 part; The zytase of 25-26 part; The pectase of 10-15 part; The amylase of 1-5 part; The lipase of 6-8 part; The tannase of 3-5 part; The coenzyme of 1-3 part; The stabilizing agent of 2-3 part; The diatomite of 3-5 part; Said stabilizing agent is made up of stabilizing agent A, stabilizing agent B and stabilizing agent C; Said stabilizing agent A accounts for 30% of stabilizer formula amount, by Ca
2+Form with polyvinylpyrrolidone; Said stabilizing agent B accounts for 50% of stabilizer formula amount, is made up of EDTA and dithiothreitol (DTT); Said stabilizing agent C accounts for 20% of stabilizer formula amount, is made up of polyvinylpyrrolidone and EDTA.Ca among the wherein said stabilizing agent A
2+With the weight ratio of polyvinylpyrrolidone be 8.5: 1; The weight ratio of EDTA and dithiothreitol (DTT) is 1: 35 among the said stabilizing agent B; The weight ratio of polyvinylpyrrolidone and EDTA is 75: 1 among the said stabilizing agent C.Wherein coenzyme can be selected the active coenzyme of various enzyme performances in the promotion multienzyme compound formulation commonly used of this area as required.
Wherein the enzyme of laccase is lived and is 3000U/g, and the enzyme work of zytase is 2,000,000 U/g, and the enzyme work of pectase is 900,000 U/g, and diastatic enzyme work is 800,000 U/g, and the enzyme of lipase is lived and is 25000U/g, and the enzyme work of tannase is 200 tannase units/gram.
Multienzyme compound formulation preparation process is following:
1) the pH value with water transfers to 7.5-9, adds amylase, lipase, tannase successively and stirs adding stabilizing agent A, mixing;
2) in the mixture that step 1) obtains, add successively after pectase, zytase, laccase stir, add formula ratio 60% coenzyme and stabilizing agent B, mixing left standstill 5-10 minute;
3) in step 2) add coenzyme and the diatomite mixing of formula ratio 20% in the mixture that obtains, place and leave standstill after 5-36 hour packing under the 4-6 ℃ of condition and store;
4) before the multienzyme compound formulation uses 0.5-1 hour, the coenzyme and the stabilizing agent C of adding surplus in the mixture that step 3) obtains, mixing.
Above-mentioned multienzyme compound formulation and specific preparation method thereof make this multienzyme compound formulation stable preservation over a period to come, and still have good vigor in use.
Use the above-mentioned multienzyme compound formulation for preparing to prepare fibre stuff, comprising from above-mentioned 100kg straw:
1) uses the liquid of discongesting that forms by said multienzyme compound formulation thin up at room temperature straw to be carried out preimpregnation, make straw discongested liquid and soak into fully, obtain the preimpregnation mixture; Total restatement with the preimpregnation mixture; The content of said straw is 25-30wt%; The pH value of regulating above-mentioned preimpregnation mixture then is to 7.5-11; In not being higher than 65 ℃ temperature range, said preimpregnation mixture discongested through 16-18 hour obtains discongesting straw completely, through detect method that inserted link inserts the resistance size judge discongest whether complete;
2) add fabric softening and cationic surfactant in the mixture above-mentioned discongesting, the said mixture of discongesting is carried out 30-60 minute softening and slaking, obtain mixture softening and slaking at 60-80 ℃;
Can judge to discongest to soften whether carry out fully through the method for detection fibers broken resistance intensity with slaking; The kind of said fabric softening and cationic surfactant and addition can be selected conventional kind and the addition of adding in this area.The concentration of discongesting the mixture solid content that is used for fiber softening and maturation stage can rule of thumb be selected, as long as can make softening and slaking;
3) above-mentioned softening mixture with slaking is ground separate, obtain the rice-straw fibre slurry, the concentration that is used for grinding the mixture solid content of the softening and slaking of separating step can rule of thumb be selected, as long as mill is separated.Wherein the mill process of separating uses high dense mill to carry out; Fixedly the gap between mill and the rotary grinding disk is 2-3mm; Motor speed is 2000 rev/mins, treats to stop when the straw mill is separated beating degree for 20-25, then through conventional dilution pastime and the concentrated rice-straw fibre slurry that obtains the 73kg amount; Can know that according to the beating degree index above-mentioned fibre stuff can be used for the production of agricultural product packaging with paper.Use physical sedimentation and flocculation technique from the waste water for preparing slurry and obtain, to reclaim lignin, the yield of lignin is 16kg, and content of lignin is approximately 200g and can knows that the rate of recovery of present embodiment method lignin is 80% from the 1kg straw.
When the multienzyme compound formulation that present embodiment provides is prepared the 73kg fibre stuff from above-mentioned 100kg straw; Reclaimed the lignin of 16kg; The method that process is not used the high-temperature high-pressure chemical boiling for preparing at slurry; The fibre stuff that obtains can reach the production requirement of agricultural product packaging with paper, and can be used for preparing and do not have chemical residual, environment friendly and pollution-free packing box or paper products.
Embodiment 2, utilize multienzyme compound formulation of the present invention to obtain fibre stuff from maize straw
To the maize straw of 100kg, the multienzyme compound formulation that present embodiment uses is prepared from for the water that the multienzyme complex 1kg adds 45-65kg, and the prescription of said multienzyme complex comprises the component of following weight portion: the laccase of 30-31 part; The zytase of 27-28 part; The pectase of 15-20 part; The amylase of 3-5 part; 0.5-3 the lipase of part; 0.1-1 the tannase of part; 0.5-1 the coenzyme of part; The stabilizing agent of 1-3 part; The diatomite of 10-15 part; Said stabilizing agent is made up of stabilizing agent A, stabilizing agent B and stabilizing agent C; Said stabilizing agent A accounts for 30% of stabilizer formula amount, by Ca
2+Form with polyvinylpyrrolidone; Said stabilizing agent B accounts for 50% of stabilizer formula amount, is made up of EDTA and dithiothreitol (DTT); Said stabilizing agent C accounts for 20% of stabilizer formula amount, is made up of polyvinylpyrrolidone and EDTA; Ca among the said stabilizing agent A
2+With the weight ratio of polyvinylpyrrolidone be 9.5: 1; The weight ratio of EDTA and dithiothreitol (DTT) is 1: 45 among the said stabilizing agent B; The weight ratio of polyvinylpyrrolidone and EDTA is 85: 1 among the said stabilizing agent C.
Said coenzyme is the nicotinic acid in the B family vitamin, and the purity of said nicotinic acid is 99.6%.
Wherein the enzyme of laccase is lived and is 3000U/g, and the enzyme work of zytase is 2,000,000 U/g, and the enzyme work of pectase is 900,000 U/g, and diastatic enzyme work is 800,000 U/g, and the enzyme of lipase is lived and is 25000U/g, and the enzyme work of tannase is 200 tannase units/gram.
Multienzyme compound formulation preparation process is following:
1) the pH value with water transfers to 7.5-9, adds amylase, lipase, tannase successively and stirs adding stabilizing agent A, mixing;
2) in the mixture that step 1) obtains, add successively after pectase, zytase, laccase stir, add formula ratio 60% coenzyme and stabilizing agent B, mixing left standstill 5-10 minute;
3) in step 2) add coenzyme and the diatomite mixing of formula ratio 20% in the mixture that obtains, place and leave standstill after 5-36 hour packing under the 4-6 ℃ of condition and store;
4) before the multienzyme compound formulation uses 0.5-1 hour, the coenzyme and the stabilizing agent C of adding surplus in the mixture that step 3) obtains, mixing.
Above-mentioned multienzyme compound formulation and specific preparation method thereof make this multienzyme compound formulation stable preservation over a period to come, and still have good vigor in use.
Maize straw described in the present embodiment has also carried out getting the raw materials ready before the fibre stuff in preparation, dedusting removal of impurities and brooming step process; Wherein said material preparation step is included as the process with the maize straw segment, and said brooming step is included as maize straw is processed into thread process.
Use the above-mentioned multienzyme compound formulation for preparing to prepare fibre stuff, comprising from above-mentioned 100kg maize straw:
1) uses the liquid of discongesting that forms by said multienzyme compound formulation thin up at room temperature maize straw to be carried out preimpregnation, make maize straw discongested liquid and soak into fully, obtain the preimpregnation mixture; Total restatement with the preimpregnation mixture; The content of said maize straw is 25-30wt%; The pH value of regulating above-mentioned preimpregnation mixture then is to 7.5-11, in not being higher than 65 ℃ temperature range, said preimpregnation mixture carried out discongesting in 6-18 hour obtaining discongesting maize straw completely; Promptly discongest mixture, insert the method judgement of resistance size through inserted link and discongest whether fully;
2) add fabric softening and cationic surfactant in the mixture simultaneously above-mentioned discongesting, the said mixture of discongesting is carried out 30-60 minute softening and slaking, obtain mixture softening and slaking at 60-80 ℃;
Can judge to discongest to soften whether carry out fully through detection fibers broken resistance intensity method with slaking; The weight ratio of said fabric softening and maize straw is 1-2: 100, and the weight ratio of said cationic surfactant and maize straw is 1-2: 100, and the mixture solid concentration of discongesting that control is used for fiber softening and maturation stage is 35-40wt%; Said cationic surfactant can be cationic surfactants such as fatty amine salt, ethanolamine salt, polyethylene polyamines salt and quaternary ammonium salt; Said fabric softening can be string softening agents such as imidazoline.
3) above-mentioned softening mixture with slaking is ground separate, obtain the maize straw fibre stuff, and the solid concentration that control is used for grinding the mixture of the softening and slaking of separating step is 15-35wt%; Wherein the mill process of separating uses high dense mill to carry out twice, is corase grind for the first time, and fixedly the gap between mill and the rotary grinding disk is 2-3mm; Motor speed is 2000 rev/mins, treats to stop when the maize straw mill is separated beating degree 20-25, is correct grinding for the second time; Fixedly the gap between mill and the rotary grinding disk is 1-1.5mm; Motor speed is 2000 rev/mins, treats to stop when the maize straw mill is separated beating degree 30-35, then through conventional dilution pastime and the concentrated maize straw fibre stuff that obtains the 30kg amount; Can know that according to the beating degree index above-mentioned fibre stuff can be used for producing paper pulp or paper behaviour makes.Use physical sedimentation and flocculation technique from the waste water for preparing slurry and obtain, to reclaim lignin, the yield of lignin is 9kg, and content of lignin is approximately 100g and can knows that the rate of recovery of present embodiment method lignin is 90% from the 1kg maize straw.
When the multienzyme compound formulation that present embodiment provides is prepared the 30kg fibre stuff from above-mentioned 100kg maize straw; Reclaimed the lignin of 9kg; The method that process is not used the high-temperature high-pressure chemical boiling for preparing at slurry; The fibre stuff that obtains can reach produces the requirement that paper pulp or paper behaviour make, and can be used for preparation and do not have chemical residual, environment friendly and pollution-free packing box or paper products.
Embodiment 3, utilize multienzyme compound formulation of the present invention to obtain fibre stuff from wheat straw
To the wheat straw of 100kg, the multienzyme compound formulation that present embodiment uses is prepared from for the water that the multienzyme complex 0.85kg adds 43-50kg, and the prescription of said multienzyme complex comprises the component of following weight portion: 33 parts laccase; The zytase of 25-26 part; The pectase of 10-11 part; The amylase of 1-3 part; The lipase of 8-9 part; The tannase of 3-5 part; 0.1-1 the coenzyme of part; The stabilizing agent of 1-3 part; The diatomite of 3-10 part; Said stabilizing agent is made up of stabilizing agent A, stabilizing agent B and stabilizing agent C; Said stabilizing agent A accounts for 30% of stabilizer formula amount, by Ca
2+Form with polyvinylpyrrolidone; Said stabilizing agent B accounts for 50% of stabilizer formula amount, is made up of EDTA and dithiothreitol (DTT); Said stabilizing agent C accounts for 20% of stabilizer formula amount, is made up of polyvinylpyrrolidone and EDTA; Ca among the said stabilizing agent A
2+With the weight ratio of polyvinylpyrrolidone be 9: 1; The weight ratio of EDTA and dithiothreitol (DTT) is 1: 40 among the said stabilizing agent B; The weight ratio of polyvinylpyrrolidone and EDTA is 80: 1 among the said stabilizing agent C.Said coenzyme is made up of biotin in the B family vitamin and riboflavin; Wherein the purity of biotin is 99.2%; The purity of riboflavin is 98.5%; Can carry out suitable choice to biotin in the coenzyme and riboflavin proportion of composing according to the characteristic of the employed various enzymes of present embodiment, those skilled in the art can accomplish based on its rudimentary knowledge, and present embodiment is not done particular determination for concrete portfolio ratio.
Wherein the enzyme of laccase is lived and is 3000U/g, and the enzyme work of zytase is 2,000,000 U/g, and the enzyme work of pectase is 900,000 U/g, and diastatic enzyme work is 800,000 U/g, and the enzyme of lipase is lived and is 25000U/g, and the enzyme work of tannase is 200 tannase units/gram.
Multienzyme compound formulation preparation process is following:
1) the pH value with water transfers to 7.5-9, adds amylase, lipase, tannase successively and stirs adding stabilizing agent A, mixing;
2) in the mixture that step 1) obtains, add successively after pectase, zytase, laccase stir, add formula ratio 60% coenzyme and stabilizing agent B, mixing left standstill 5-10 minute;
3) in step 2) add coenzyme and the diatomite mixing of formula ratio 20% in the mixture that obtains, place and leave standstill after 5-36 hour packing under the 4-6 ℃ of condition and store;
4) before the multienzyme compound formulation uses 0.5-1 hour, the coenzyme and the stabilizing agent C of adding surplus in the mixture that step 3) obtains, mixing.
Above-mentioned multienzyme compound formulation and specific preparation method thereof make this multienzyme compound formulation stable preservation over a period to come, and still have good vigor in use.
Wheat straw described in the present embodiment has also carried out getting the raw materials ready before the fibre stuff in preparation, dedusting removal of impurities and brooming step process, and wherein said material preparation step is included as the process with the wheat straw segment, and said brooming step is included as wheat straw is processed into thread process.In the brooming wheat straw, wheat straw is sprayed water, making the dry weight of wheat straw in the wheat straw behind the water spray is 50-60wt%.
Use the above-mentioned multienzyme compound formulation for preparing to prepare fibre stuff, comprising from above-mentioned 100kg wheat straw:
1) uses the liquid of discongesting that forms by said multienzyme compound formulation thin up at room temperature wheat straw to be carried out preimpregnation, make wheat straw discongested liquid and soak into fully, obtain the preimpregnation mixture; Total restatement with the preimpregnation mixture; The content of said wheat straw is 25-30wt%; The pH value of regulating above-mentioned preimpregnation mixture then is to 7.5-11, in not being higher than 65 ℃ temperature range, said preimpregnation mixture carried out discongesting in 16-18 hour obtaining discongesting wheat straw completely; Promptly discongest mixture, discongest whether fully through the method judgement that detects inserted link insertion resistance size;
2) add fabric softening and cationic surfactant in the mixture simultaneously above-mentioned discongesting, the said mixture of discongesting is carried out 30-60 minute softening and slaking, obtain mixture softening and slaking at 60-80 ℃;
Can judge to discongest to soften whether carry out fully through the method for detection fibers broken resistance intensity with slaking; The weight ratio of said fabric softening and wheat straw is 1-2: 100, and the weight ratio of said cationic surfactant and wheat straw is 1-2: 100, and the mixture solid concentration of discongesting that control is used for fiber softening and maturation stage is 35-40wt%; Said cationic surfactant can be cationic surfactants such as fatty amine salt, ethanolamine salt, polyethylene polyamines salt and quaternary ammonium salt; Said fabric softening can be string softening agents such as imidazoline;
3) above-mentioned softening mixture with slaking is ground separate, obtain the wheat straw fiber slurry, and the solid concentration that control is used for grinding the mixture of the softening and slaking of separating step is 15-35wt%; Wherein the mill process of separating uses high dense mill to carry out twice, is corase grind for the first time, and fixedly the gap between mill and the rotary grinding disk is 2-3mm; Motor speed is 2000 rev/mins, treats to stop when the wheat straw mill is separated beating degree 20-25, is correct grinding for the second time; Fixedly the gap between mill and the rotary grinding disk is 1-1.5mm; Motor speed is 2000 rev/mins, treats to stop when the wheat straw mill is separated beating degree 30-35, then through conventional dilution pastime and the concentrated wheat straw fiber slurry that obtains the 74kg amount; Can know that according to the beating degree index above-mentioned fibre stuff can be used for producing paper pulp or paper behaviour makes.Use physical sedimentation and flocculation technique from the waste water for preparing slurry and obtain, to reclaim lignin, the yield of lignin is 16kg, and content of lignin is approximately 250g and can knows that the rate of recovery of present embodiment method lignin is 64% from the 1kg wheat straw.
When the multienzyme compound formulation that present embodiment provides is prepared the 74kg fibre stuff from above-mentioned 100kg wheat straw; Reclaimed the lignin of 16kg; The method that process is not used the high-temperature high-pressure chemical boiling for preparing at slurry; The fibre stuff that obtains can reach produces the requirement that paper pulp or paper behaviour make, and can be used for preparation and do not have chemical residual, environment friendly and pollution-free packing box or paper products.
Claims (10)
1. a multienzyme compound formulation is characterized in that, it is formulated to add water by multienzyme complex, and wherein the weight ratio of multienzyme complex and water is 1:50-100, and the prescription of said multienzyme complex comprises the component of following weight portion: the laccase of 30-35 part; The zytase of 25-35 part; The pectase of 10-20 part; 0.1-10 the amylase of part; 0.3-10 the lipase of part; 0.1-5 the tannase of part; 0.1-3 the coenzyme of part; 0.1-3 the stabilizing agent of part; 0.3-25 the diatomite of part;
Said stabilizing agent is made up of stabilizing agent A, stabilizing agent B and stabilizing agent C;
Said stabilizing agent A accounts for 30% of stabilizer formula amount, by Ca
2+Stabilizing agent and polyvinylpyrrolidone are formed, said Ca
2+Stabilizing agent is by any Ca that provides
2+Material provide;
Said stabilizing agent B accounts for 50% of stabilizer formula amount, is made up of EDTA and dithiothreitol (DTT);
Said stabilizing agent C accounts for 20% of stabilizer formula amount, is made up of polyvinylpyrrolidone and EDTA.
2. multienzyme compound formulation according to claim 1 is characterized in that, Ca among the said stabilizing agent A
2+The weight ratio of stabilizing agent and polyvinylpyrrolidone is 8-10:1, said Ca
2+Stabilizing agent is by any Ca that provides
2+Material provide;
The weight ratio of EDTA and dithiothreitol (DTT) is 1:30-50 among the said stabilizing agent B;
The weight ratio of polyvinylpyrrolidone and EDTA is 70-90:1 among the said stabilizing agent C.
3. multienzyme compound formulation according to claim 1 is characterized in that, said coenzyme is made up of in nicotinic acid, folic acid, biotin or the riboflavin one or more.
4. the preparation method of each described multienzyme compound formulation of claim 1-3 comprises:
1) the pH value with water transfers to 7.5-9, adds amylase, lipase, tannase and stirs adding stabilizing agent A, mixing;
2) in the mixture that step 1) obtains, add after pectase, zytase, laccase stir, add the coenzyme and the stabilizing agent B of formula ratio 60%, mixing left standstill 5-10 minute;
3) in step 2) add coenzyme and the diatomite mixing of formula ratio 20% in the mixture that obtains, place and leave standstill after 5-36 hour packing under the 4-6 ℃ of condition and store;
4) before the multienzyme compound formulation uses 0.5-1 hour, the coenzyme and the stabilizing agent C of adding surplus in the mixture that step 3) obtains, mixing.
5. each described multienzyme compound formulation of claim 1-3 is utilizing agricultural wastes to obtain the application in the fibre stuff.
6. utilize the method for each described multienzyme compound formulation of claim 1-3, may further comprise the steps from agricultural wastes acquisition fibre stuff:
1) use the liquid of discongesting that forms by said multienzyme compound formulation thin up at room temperature agricultural wastes to be carried out preimpregnation and obtain the preimpregnation mixture; Wherein saidly discongest that the weight ratio of multienzyme complex and said agricultural wastes is 0.7-1:100 in the liquid; And with total restatement of said preimpregnation mixture, the content of said agricultural wastes is 25-30wt%; The pH value of regulating above-mentioned preimpregnation mixture is to 7.5-11, in not being higher than 65 ℃ temperature range, said preimpregnation mixture carried out at least 6 hours discongesting, and obtains discongesting mixture;
2) add fabric softening and cationic surfactant in the mixture above-mentioned discongesting, the said mixture of discongesting is carried out 30-60 minute softening and slaking, obtain mixture softening and slaking at 60-80 ℃;
3) above-mentioned softening mixture with slaking is ground separate, obtain the fibre stuff of agricultural wastes.
7. method according to claim 6; It is characterized in that; Said agricultural wastes for through get the raw materials ready, the agricultural wastes of dedusting removal of impurities and brooming step process; Wherein said material preparation step comprises the process with the agricultural wastes segment, and said brooming step comprises agricultural wastes are processed into thread process.
8. method according to claim 6 is characterized in that, the weight ratio of said fabric softening and said agricultural wastes is 1-2:100, and the weight ratio of said cationic surfactant and said agricultural wastes is 1-2:100.
9. according to each described method of claim 6-8, it is characterized in that the concentration of discongesting the mixture solid content that is used for softening with slaking is 35-40wt%; The concentration that is used for grinding the mixture solid content of the softening and slaking of separating is 15-35wt%.
10. fibre stuff, said fibre stuff is prepared from according to each described method of claim 6-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100289126A CN102162198B (en) | 2011-01-26 | 2011-01-26 | Multienzyme complex preparation and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100289126A CN102162198B (en) | 2011-01-26 | 2011-01-26 | Multienzyme complex preparation and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102162198A CN102162198A (en) | 2011-08-24 |
| CN102162198B true CN102162198B (en) | 2012-11-28 |
Family
ID=44463630
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100289126A Expired - Fee Related CN102162198B (en) | 2011-01-26 | 2011-01-26 | Multienzyme complex preparation and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102162198B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103898789B (en) * | 2012-12-27 | 2016-08-24 | 东莞市绿微康生物科技有限公司 | Improve biological enzyme formulation and the application thereof of APMP paper strength |
| CN103555702B (en) * | 2013-10-19 | 2015-12-09 | 沅江浣溪沙酶技术有限公司 | The production method of chemical-mechanical pulping complex enzyme liquid and application |
| CN105250234B (en) * | 2015-09-10 | 2018-08-07 | 李厚山 | A kind of capsule shells and preparation method thereof |
| CN106049186A (en) * | 2016-05-25 | 2016-10-26 | 安徽省丰蓼农业开发有限公司 | Manufacturing method of waterproof cardboard for packaging and transportation of melons, fruits and vegetables |
| CN106244573A (en) * | 2016-08-02 | 2016-12-21 | 长兴锦龙纸业有限公司 | A kind of corn straw slurrying complex enzyme liquid and application thereof |
| CN106222153A (en) * | 2016-08-02 | 2016-12-14 | 长兴锦龙纸业有限公司 | A kind of employing straw slurry complex enzyme liquid and application thereof |
| CN106191021A (en) * | 2016-08-02 | 2016-12-07 | 长兴锦龙纸业有限公司 | A kind of ryegrass pulping complex enzyme liquid and application thereof |
| CN114182361A (en) * | 2021-11-08 | 2022-03-15 | 宜兴市舜昌亚麻纺织有限公司 | Composite biological enzyme degumming agent suitable for flax fiber supercritical degumming |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1344820A1 (en) * | 2000-11-21 | 2003-09-17 | Meiji Seika Kaisha Ltd. | Zygomycetes-origin endoglucanase lacking cellulose-binding domain |
| CN1986785A (en) * | 2005-12-23 | 2007-06-27 | 上海复星长征医学科学有限公司 | Enzyme combining stabilizer |
| CN101624583A (en) * | 2008-07-07 | 2010-01-13 | 上海纤化生物科技有限公司 | Process for manufacturing energy-saving and environment-friendly cooking and bleaching compound enzyme preparation for weaving |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007008776A1 (en) * | 2005-07-11 | 2007-01-18 | Genencor International, Inc. | Enzyme fabric care tablets for consumers and methods |
-
2011
- 2011-01-26 CN CN2011100289126A patent/CN102162198B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1344820A1 (en) * | 2000-11-21 | 2003-09-17 | Meiji Seika Kaisha Ltd. | Zygomycetes-origin endoglucanase lacking cellulose-binding domain |
| CN1986785A (en) * | 2005-12-23 | 2007-06-27 | 上海复星长征医学科学有限公司 | Enzyme combining stabilizer |
| CN101624583A (en) * | 2008-07-07 | 2010-01-13 | 上海纤化生物科技有限公司 | Process for manufacturing energy-saving and environment-friendly cooking and bleaching compound enzyme preparation for weaving |
Non-Patent Citations (1)
| Title |
|---|
| 董毅等.复合纤维素酶处理改善磨木浆质量的研究.《黑龙江造纸》.2009,(第2期),第8-11,14页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102162198A (en) | 2011-08-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102162198B (en) | Multienzyme complex preparation and preparation method and application thereof | |
| DE69635700T3 (en) | New endoglucanase | |
| US20170275213A1 (en) | Composite microorganism enzyme, method for preparing plant nutrient solution by using composite microorganism enzyme, and fertilizer synergist | |
| CN100494566C (en) | Biocatalytic clean pulping agent and application process thereof | |
| CN104293858B (en) | A kind of preparation method of nano micro crystal cellulose | |
| CN102174758B (en) | Method for preparing packaging material | |
| CN102337687A (en) | Novel pulping process for obtaining high performance fiber at high yield from plants | |
| CN102978986A (en) | Method for preparing paper pulp by using biological enzyme | |
| CN104499336A (en) | Bleaching compound enzyme for papermaking and preparation method of bleaching compound enzyme | |
| CN101857639B (en) | Method for preparing sodium carboxymethylcellulose from residues generated by producing biobutanol from maize straw | |
| CN108978305A (en) | A kind of complex enzyme formulation and the technique for preparing paper pulp | |
| CN107313241A (en) | A kind of cowboy washes neutral ferment powder and its application with resist printing | |
| CN107740305A (en) | A kind of method that stalk prepares high-strength corrugated base paper | |
| CN103082393B (en) | Preparation method of tobacco flakes through tobacco stems using biochemical machinery method | |
| CN101130934A (en) | Colored cotton linter pulp used for producing viscose fiber and method for making the same | |
| CN101792980B (en) | Mechanical pulping method for biological bacteria liquid | |
| CN101831822B (en) | Method for producing paper pulp by adopting cotton stalk peel as raw material | |
| CN108004821A (en) | A kind of regenerated papermaking technique | |
| CN100510246C (en) | Method for making paper-pulp by cold immersion for paper-making | |
| CN105648544A (en) | Composite preparation for degumming of ramie with skins and degumming method | |
| CN109423902A (en) | A kind of organic fertilizer and preparation method thereof that the black liquid generated using soda pulping process technique is prepared | |
| CN107956177A (en) | A kind of method and system for preparing paper pulp using grass type stalk as raw material | |
| CN104499337A (en) | Bleached compound enzyme and preparation method thereof | |
| CN104846680A (en) | Papermaking method | |
| CN107568486A (en) | A kind of method that pig feed is produced using bagasse |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121128 Termination date: 20150126 |
|
| EXPY | Termination of patent right or utility model |