CN102162198A - Multienzyme complex preparation and preparation method and application thereof - Google Patents
Multienzyme complex preparation and preparation method and application thereof Download PDFInfo
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- CN102162198A CN102162198A CN 201110028912 CN201110028912A CN102162198A CN 102162198 A CN102162198 A CN 102162198A CN 201110028912 CN201110028912 CN 201110028912 CN 201110028912 A CN201110028912 A CN 201110028912A CN 102162198 A CN102162198 A CN 102162198A
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Abstract
The invention provides a multienzyme complex preparation and a preparation method and application thereof. The multienzyme complex preparation is characterized by being prepared by adding water to multienzyme complex, wherein the weight ratio of the multienzyme complex to the water is 1:(50-100); and the multienzyme complex comprises the following components in parts by weight: 30-35 parts of laccase, 25-35 parts of xylanase, 10-20 parts of pectinase, 0.1-10 parts of amylase, 0.3-10 parts of lipase, 0.1-5 parts of tannase, 0.1-3 parts of coenzyme, 0.1-3 parts of stabilizing agent and 0.3-25 parts of diatomite. The multienzyme complex preparation can be applied to the preparation process of the fiber pulp for papermaking, the fiber pulp in accordance with the papermaking production requirement can be obtained without further adopting the high-temperature/pressure chemical cooking method in the pulping process, and the original lignin can be preserved to the greatest extent in the fiber pulp preparing process.
Description
Technical field
The present invention relates to a kind of biologic product and its production and application, relate in particular to a kind of multienzyme compound formulation and its production and application.
Background technology
Increasing in recent years biologic product is widely used in the industrial production field, it has significantly reduced the pollution to environment when replacing chemicals to play a role, for example at the paper industry production field, utilize enzyme preparation preliminary treatment chemical pulping method to replace the pure chemistry pulp-making method, reduced the use amount of chemicals in the pure chemistry pulp-making method dramatically, and pulping process has the sewage emissions of high BOD, COD load and hypertoxic, strong carcinogenic material.
Yet, existing enzyme preparation preliminary treatment chemical pulping method is after at first using enzyme preparation that material is carried out preliminary treatment, still need further to adopt the method for high-temperature high-pressure chemical boiling (mainly being alkaline boiling) could obtain to meet the fibre stuff that paper requires, wherein, farthest destroyed at chemical steaming process lignin, make to be difficult to after the slurrying lignin separated further from waste liquid and utilize, for example: be used for the production of functional biological fertilizer; And the produced slurry of above-mentioned enzyme preparation preliminary treatment chemical pulping method is used to make packing box or paper products, because of using chemicals in preparation slurry process, unavoidably contain chemical residual in packing box that it is prepared or the paper products, exist safe in utilization and degraded after stain problem of environment, therefore need further processing just can address the above problem, therefore cause the raising of the processing cost of paper products such as packing box, make production technology complicated more, be unfavorable for large-scale production and processing.
How therefore a kind of new enzyme preparation be provided, making does not need further to adopt the step of high-temperature high-pressure chemical boiling at pulping process, just can obtain to meet the fibre stuff that paper requires, and when producing fibre stuff, at utmost keep the lignin of ecosystem, and make production process cleaning, energy-conservation, efficiently become problem to be solved arranged.
Summary of the invention
The invention provides a kind of multienzyme compound formulation, it uses the proportioning of specific enzyme preparation, stabilizing agent and coenzyme to make multienzyme compound formulation storage-stable over a period to come, and still has good vigor in use.
The present invention also provides the preparation method of multienzyme compound formulation, specific addition and interpolation order by different enzyme preparations, stabilizing agent and coenzyme, make that the multienzyme compound formulation prepare over a period to come can storage-stable, and still have good vigor in use.
The present invention also provides the application of above-mentioned multienzyme compound formulation in utilizing agricultural wastes acquisition fibre stuff, use this multienzyme compound formulation, the preparation process of whole fibre stuff does not need to use chemicals, can obtain to meet the fibre stuff that paper requires.
The present invention also provides the method for utilizing described multienzyme compound formulation to obtain fibre stuff from agricultural wastes, by above-mentioned multienzyme compound formulation being applied to the preparation process of fibre stuff, need not adopt the method for high-temperature high-pressure chemical boiling at pulping process, just can obtain to meet the fibre stuff that paper requires, and when producing fibre stuff, can at utmost keep the lignin of ecosystem.
The present invention further also provides a kind of fibre stuff, and described fibre stuff is prepared from according to the method described above; Described slurry can be used for preparation does not have chemical residual, environment friendly and pollution-free packing box or paper products.
Multienzyme compound formulation provided by the invention, it is formulated to add water by multienzyme complex, and wherein, the weight ratio of multienzyme complex and water is 1: 50-100; The prescription of described multienzyme complex comprises the component of following weight portion: the laccase of 30-35 part; The zytase of 25-35 part; The pectase of 10-20 part; 0.1-10 the amylase of part; 0.3-10 the lipase of part; 0.1-5 the tannase of part; 0.1-3 the coenzyme of part; 0.1-3 the stabilizing agent of part; 0.3-25 the diatomite of part; Described stabilizing agent is made up of stabilizing agent A, stabilizing agent B and stabilizing agent C;
Described stabilizing agent A accounts for 30% of stabilizer formula amount, by Ca
2+Form with polyvinylpyrrolidone;
Described stabilizing agent B accounts for 50% of stabilizer formula amount, is made up of EDTA and dithiothreitol (DTT);
Described stabilizing agent C accounts for 20% of stabilizer formula amount, is made up of polyvinylpyrrolidone and EDTA.
In one embodiment of the invention, Ca among the described stabilizing agent A
2+With the weight ratio of polyvinylpyrrolidone be 8-10: 1; The weight ratio of EDTA and dithiothreitol (DTT) is 1 among the described stabilizing agent B: 30-50; The weight ratio of polyvinylpyrrolidone and EDTA is 70-90 among the described stabilizing agent C: 1;
Described coenzyme is made up of in nicotinic acid, folic acid, biotin or the riboflavin one or more.
The preparation method of described multienzyme compound formulation comprises:
1) the pH value with water transfers to 7.5-9, adds amylase, lipase, tannase and stirs adding stabilizing agent A, mixing;
2) add in the mixture that step 1) obtains after pectase, zytase, laccase stir, add the coenzyme and the stabilizing agent B of formula ratio 60%, mixing left standstill 5-10 minute;
3) in step 2) add the coenzyme and the diatomite mixing of formula ratio 20% in the mixture that obtains, place and leave standstill packing storage after 5-36 hour under the 4-6 ℃ of condition;
4) before the multienzyme compound formulation uses 0.5-1 hour, the coenzyme and the stabilizing agent C of adding formula ratio 20% in the mixture that step 3) obtains, mixing.
Ca in the above-mentioned multienzyme compound formulation
2+Stabilizing agent is by any Ca that provides
2+Material provide, for example: CaCl
2, CaSO
4, CaNO
3Deng; Ca in the described multienzyme compound formulation
2+The amount of stabilizing agent is by CaCl
2The amount of calculating is as selecting CaSO
4, CaNO
3Deng, then press with equivalent CaCl
2Conversion Calculation goes out the weight portion that corresponding calcium salt uses.Stabilizing agent A is now with the current, leaves standstill after stabilizing agent B prepares after 5-10 minute to use.In the described coenzyme, generally select purity greater than 99.5% nicotinic acid, purity greater than 99% biotin, purity greater than 98% riboflavin and purity greater than 98% folic acid.
Among the preparation method of above-mentioned multienzyme compound formulation, the weight ratio of multienzyme complex and water, and step 1)-3) effect of adding stabilizing agent and coenzyme in is in order to guarantee that the mixture that step 3) obtains can store 20-25 days at ambient temperature, the activity that keeps various enzymes in the multienzyme compound formulation simultaneously, the effect that limits before use (that is, thin up is for before discongesting liquid) 0.5-1 hour interpolation stabilizing agent and coenzyme in the step 4) is in order to guarantee various enzymes activity in use in the multienzyme compound formulation.So, according to the characteristic of various enzymes used in the present invention the kind of coenzyme and consumption and proportion of composing are carried out suitable selection, those skilled in the art can finish based on its rudimentary knowledge, when adopting multiple coenzyme to be used in combination, the present invention does not do particular determination for concrete portfolio ratio.
Based on above-mentioned multienzyme compound formulation, the present invention further provides the application of above-mentioned multienzyme compound formulation in utilizing agricultural wastes acquisition fibre stuff; And utilize above-mentioned multienzyme compound formulation to obtain the method for fibre stuff from agricultural wastes.Described agricultural wastes are stalk, root, the stem of crops in the agriculture and forestry production process, and fibrous part such as limb is as straw, maize straw, wheat straw, straw, cotton stalk etc.
Provided by the inventionly utilize above-mentioned multienzyme compound formulation to may further comprise the steps from the method that agricultural wastes obtain fibre stuff:
1) use the liquid of discongesting that forms by described multienzyme compound formulation thin up at room temperature agricultural wastes to be carried out preimpregnation and obtain the preimpregnation mixture, wherein saidly discongest that the weight ratio of multienzyme complex and described agricultural wastes is 0.7-1 in the liquid: 100, and with total restatement of described preimpregnation mixture, the content of described agricultural wastes is 25-30wt%; The pH value of regulating above-mentioned preimpregnation mixture then is to 7.5-11, in not being higher than 65 ℃ temperature range, described preimpregnation mixture carried out at least 6 hours discongesting, and obtains discongesting mixture;
2) add fabric softening and cationic surfactant in the mixture above-mentioned discongesting, the described mixture of discongesting is carried out 30-60 minute softening and slaking, obtain the mixture of softening and slaking at 60-80 ℃;
3) above-mentioned mixture softening and slaking is ground separate, obtain the fibre stuff of agricultural wastes.
In one embodiment of the invention, described agricultural wastes for through get the raw materials ready, the agricultural wastes of dedusting removal of impurities and brooming step process, wherein said material preparation step comprises the process with the agricultural wastes segment, and described brooming step comprises agricultural wastes are processed into thread process.Further, can spray water to agricultural wastes in the brooming agricultural wastes, making the dry weight of agricultural wastes in the agricultural wastes behind the water spray is 50-60wt%.Above-mentioned melting process can use conventional chaffcutter to carry out, the dedusting removal of impurities can use conventional deduster to carry out, described brooming can use conventional brooming equipment to carry out, mill is separated process and also can be carried out once or once according to the purposes (i.e. the specification of the paper products that will make) of fibre stuff, can make conventional mill separate equipment and carry out, for example the pulping equipment in the paper technology.
In another embodiment of the present invention, the weight ratio of described fabric softening and described agricultural wastes (being preimpregnation step agricultural wastes before) is 1-2: 100, and the weight ratio of described cationic surfactant and described agricultural wastes (being preimpregnation step agricultural wastes before) is 1-2: 100; Be used for softening and the concentration of discongesting the mixture solid content of slaking is 35-40wt%; The concentration that is used for grinding the mixture solid content of the softening and slaking of separating is 15-35wt%.
Described cationic surfactant can be cationic surfactants such as fatty amine salt, ethanolamine salt, polyethylene polyamines salt and quaternary ammonium salt; Described fabric softening can be string softening agents such as imidazoline.
The present invention further also provides a kind of fibre stuff, and described fibre stuff is prepared from according to the method described above.
Provided by the invention from the method for agricultural wastes acquisition fibre stuff, fully react for making in the multienzyme compound formulation various enzyme components and agricultural wastes discongesting process, and make each enzyme component all by full consumption, at least need 6 hours, in the practice according to the difference of material or enzyme preparation amount, generally carry out to discongest in 6-72 hour fully, in industrial production, take all factors into consideration other factors such as time and cost, make the process of discongesting carry out to discongest in 6-24 hour fully, usually can pass through the agricultural wastes change color, the experiences such as resistance that feel and firmness change and taperer insert are simple judges to discongest whether finish, also can judge the degree of discongesting of raw material by microscopy, also can select other conventional meanses of this area to judge certainly as the case may be.Further, the degree softening and slaking of fiber can judge that the mill degree of separating can be judged by detecting the beating degree method by detection fibers broken resistance intensity method.
The multienzyme compound formulation of specific composition provided by the invention and specific preparation method thereof make this multienzyme compound formulation stable preservation over a period to come, and still has good vigor in use, concrete enzyme preparation in the described multienzyme compound formulation is and is purchased product, wherein the enzyme work of laccase is generally 3000U/g, the enzyme work of zytase is generally 2,000,000 U/g, the enzyme work of pectase is generally 900,000 U/g, diastatic enzyme work is generally 800,000 U/g, the enzyme work of lipase is generally 25000U/g, and the enzyme work of tannase is generally 200 tannase units/gram; Scope is different with above-mentioned scope if the enzyme of the enzyme preparation of using is lived, can convert accordingly and determine the weight portion that uses in the prescription of multienzyme complex, the method of utilizing above-mentioned multienzyme compound formulation from agricultural wastes acquisition fibre stuff provided by the invention has the following advantages:
(1) liquid of discongesting that adopts the multienzyme compound formulation to be prepared into is discongested the agriculture waste fibres, avoided the electrochemical conditions of High Temperature High Pressure strong acid-base, make fiber and lignin realization original position dissociate, rather than farthest destroy lignin structure, make lignin keep than ecosystem, also reached the enough big degree of polymerization after removing with fiber, be convenient to adopt conventional physico-chemical process just the lignin composition thoroughly therefrom can be flocculated in water treatment stage, enter the production of functional biological fertilizer, make production process accomplish cleaning, energy-conservation, efficient.
(2) by agricultural wastes are carried out brooming make split between the cell membrane of agricultural wastes from, various enzyme components in the promotion multienzyme compound formulation can enter iuntercellular fast and carry out work, estranged efficient and speed have been improved greatly, can be reduced to 16 hours even shorter the time of discongesting the agricultural wastes structure, for large-scale industrial production has been created sufficient condition.The existing technology of producing fibre stuff then needs 30-40 days time just can finish the degraded of the compositions such as lignin of 60-70%.
(3) because production process has been avoided High Temperature High Pressure strong acid, highly basic condition, and do not use chemicals, described fibre stuff can be used for suppressing environment-friendly type agricultural production packing material such as cutlery box, rice packaging box, tea caddy, vegetables packing box etc., when the packing material of producing is degraded after use, can the residual harmful substance contaminated environment yet.
(4) adopt this technology to produce, technology is simple, and the utilization of agricultural wastes material has been accomplished to utilize fully, and except cellulose components was converted into fibre stuff, other compositions transferred the raw material of producing biological organic fertilizer to, the comprehensive benefit height.In addition, equipment investment is lower more than 1/3rd than tradition, and the production cost of fibre stuff is lower more than 1/3rd than tradition.
The specific embodiment
Further specify the technical scheme of the embodiment of the invention below in conjunction with specific embodiment.Various enzymes, coenzyme and the stabilizing agent that uses in following examples all can obtain by commercial the purchase.
Embodiment 1, utilize multienzyme compound formulation of the present invention to obtain fibre stuff from straw
At the straw of 100kg, the multienzyme compound formulation that present embodiment uses is prepared from for the water that the multienzyme complex by 0.7kg adds 35-70kg, and the prescription of described multienzyme complex comprises the component of following weight portion: the laccase of 31-33 part; The zytase of 25-26 part; The pectase of 10-15 part; The amylase of 1-5 part; The lipase of 6-8 part; The tannase of 3-5 part; The coenzyme of 1-3 part; The stabilizing agent of 2-3 part; The diatomite of 3-5 part; Described stabilizing agent is made up of stabilizing agent A, stabilizing agent B and stabilizing agent C; Described stabilizing agent A accounts for 30% of stabilizer formula amount, by Ca
2+Form with polyvinylpyrrolidone; Described stabilizing agent B accounts for 50% of stabilizer formula amount, is made up of EDTA and dithiothreitol (DTT); Described stabilizing agent C accounts for 20% of stabilizer formula amount, is made up of polyvinylpyrrolidone and EDTA.Ca among the wherein said stabilizing agent A
2+With the weight ratio of polyvinylpyrrolidone be 8.5: 1; The weight ratio of EDTA and dithiothreitol (DTT) is 1: 35 among the described stabilizing agent B; The weight ratio of polyvinylpyrrolidone and EDTA is 75: 1 among the described stabilizing agent C.Wherein coenzyme can be selected the active coenzyme of various enzyme performances in the promotion multienzyme compound formulation commonly used of this area as required.
Wherein the enzyme of laccase is lived and is 3000U/g, and the enzyme work of zytase is 2,000,000 U/g, and the enzyme work of pectase is 900,000 U/g, and diastatic enzyme work is 800,000 U/g, and the enzyme of lipase is lived and is 25000U/g, and the enzyme work of tannase is 200 tannase units/gram.
Multienzyme compound formulation preparation process is as follows:
1) the pH value with water transfers to 7.5-9, adds amylase, lipase, tannase successively and stirs adding stabilizing agent A, mixing;
2) add successively in the mixture that step 1) obtains after pectase, zytase, laccase stir, add formula ratio 60% coenzyme and stabilizing agent B, mixing left standstill 5-10 minute;
3) in step 2) add the coenzyme and the diatomite mixing of formula ratio 20% in the mixture that obtains, place and leave standstill packing storage after 5-36 hour under the 4-6 ℃ of condition;
4) before the multienzyme compound formulation uses 0.5-1 hour, the coenzyme and the stabilizing agent C of adding surplus in the mixture that step 3) obtains, mixing.
Above-mentioned multienzyme compound formulation and specific preparation method thereof make this multienzyme compound formulation stable preservation over a period to come, and still have good vigor in use.
Use the above-mentioned multienzyme compound formulation for preparing to prepare fibre stuff, comprising from above-mentioned 100kg straw:
1) uses the liquid of discongesting that forms by described multienzyme compound formulation thin up at room temperature straw to be carried out preimpregnation, make straw be discongested liquid and soak into fully, obtain the preimpregnation mixture; Total restatement with the preimpregnation mixture, the content of described straw is 25-30wt%, the pH value of regulating above-mentioned preimpregnation mixture then is to 7.5-11, in not being higher than 65 ℃ temperature range, described preimpregnation mixture discongested through 16-18 hour obtains discongesting straw completely, by detect method that inserted link inserts the resistance size judge discongest whether complete;
2) add fabric softening and cationic surfactant in the mixture above-mentioned discongesting, the described mixture of discongesting is carried out 30-60 minute softening and slaking, obtain the mixture of softening and slaking at 60-80 ℃;
Can judge to discongest to soften whether carry out fully by the method for detection fibers broken resistance intensity with slaking; The kind of described fabric softening and cationic surfactant and addition can be selected conventional kind and the addition of adding in this area.The concentration of discongesting the mixture solid content that is used for fiber softening and maturation stage can rule of thumb be selected, as long as can make softening and slaking;
3) above-mentioned mixture softening and slaking is ground separate, obtain the rice-straw fibre slurry, the concentration that is used for grinding the mixture solid content of the softening and slaking of separating step can rule of thumb be selected, as long as mill is separated.Wherein the mill process of separating uses high dense mill to carry out, fixedly the gap between mill and the rotary grinding disk is 2-3mm, motor speed is 2000 rev/mins, treat to stop when the straw mill is separated beating degree for 20-25, divert oneself and concentrate through the dilution of routine then and obtain the rice-straw fibre slurry that 73kg measures, according to the beating degree index as can be known, above-mentioned fibre stuff can be used for the production of agricultural product packaging with paper.Use physical sedimentation and flocculation technique to reclaim lignin from the waste water that the preparation slurry obtains, the yield of lignin is 16kg, and content of lignin is approximately 200g as can be known from the 1kg straw, and the rate of recovery of present embodiment method lignin is 80%.
When the multienzyme compound formulation that present embodiment provides is prepared the 73kg fibre stuff from above-mentioned 100kg straw, reclaimed the lignin of 16kg, do not use the method for high-temperature high-pressure chemical boiling in the preparation process of slurry, the fibre stuff that obtains can reach the production requirement of agricultural product packaging with paper, and can be used for preparation and do not have chemical residual, environment friendly and pollution-free packing box or paper products.
Embodiment 2, utilize multienzyme compound formulation of the present invention to obtain fibre stuff from maize straw
At the maize straw of 100kg, the multienzyme compound formulation that present embodiment uses is prepared from for the water that the multienzyme complex by 1kg adds 45-65kg, and the prescription of described multienzyme complex comprises the component of following weight portion: the laccase of 30-31 part; The zytase of 27-28 part; The pectase of 15-20 part; The amylase of 3-5 part; 0.5-3 the lipase of part; 0.1-1 the tannase of part; 0.5-1 the coenzyme of part; The stabilizing agent of 1-3 part; The diatomite of 10-15 part; Described stabilizing agent is made up of stabilizing agent A, stabilizing agent B and stabilizing agent C; Described stabilizing agent A accounts for 30% of stabilizer formula amount, by Ca
2+Form with polyvinylpyrrolidone; Described stabilizing agent B accounts for 50% of stabilizer formula amount, is made up of EDTA and dithiothreitol (DTT); Described stabilizing agent C accounts for 20% of stabilizer formula amount, is made up of polyvinylpyrrolidone and EDTA; Ca among the described stabilizing agent A
2+With the weight ratio of polyvinylpyrrolidone be 9.5: 1; The weight ratio of EDTA and dithiothreitol (DTT) is 1: 45 among the described stabilizing agent B; The weight ratio of polyvinylpyrrolidone and EDTA is 85: 1 among the described stabilizing agent C.
Described coenzyme is the nicotinic acid in the B family vitamin, and the purity of described nicotinic acid is 99.6%.
Wherein the enzyme of laccase is lived and is 3000U/g, and the enzyme work of zytase is 2,000,000 U/g, and the enzyme work of pectase is 900,000 U/g, and diastatic enzyme work is 800,000 U/g, and the enzyme of lipase is lived and is 25000U/g, and the enzyme work of tannase is 200 tannase units/gram.
Multienzyme compound formulation preparation process is as follows:
1) the pH value with water transfers to 7.5-9, adds amylase, lipase, tannase successively and stirs adding stabilizing agent A, mixing;
2) add successively in the mixture that step 1) obtains after pectase, zytase, laccase stir, add formula ratio 60% coenzyme and stabilizing agent B, mixing left standstill 5-10 minute;
3) in step 2) add the coenzyme and the diatomite mixing of formula ratio 20% in the mixture that obtains, place and leave standstill packing storage after 5-36 hour under the 4-6 ℃ of condition;
4) before the multienzyme compound formulation uses 0.5-1 hour, the coenzyme and the stabilizing agent C of adding surplus in the mixture that step 3) obtains, mixing.
Above-mentioned multienzyme compound formulation and specific preparation method thereof make this multienzyme compound formulation stable preservation over a period to come, and still have good vigor in use.
Maize straw described in the present embodiment has also carried out getting the raw materials ready before the fibre stuff in preparation, dedusting removal of impurities and brooming step process, wherein said material preparation step is included as the process with the maize straw segment, and described brooming step is included as maize straw is processed into thread process.
Use the above-mentioned multienzyme compound formulation for preparing to prepare fibre stuff, comprising from above-mentioned 100kg maize straw:
1) uses the liquid of discongesting that forms by described multienzyme compound formulation thin up at room temperature maize straw to be carried out preimpregnation, make maize straw be discongested liquid and soak into fully, obtain the preimpregnation mixture; Total restatement with the preimpregnation mixture, the content of described maize straw is 25-30wt%, the pH value of regulating above-mentioned preimpregnation mixture then is to 7.5-11, in not being higher than 65 ℃ temperature range, described preimpregnation mixture carried out discongesting in 6-18 hour obtain discongesting maize straw completely, promptly discongest mixture, insert the method judgement of resistance size by inserted link and discongest whether fully;
2) add fabric softening and cationic surfactant in the mixture simultaneously above-mentioned discongesting, the described mixture of discongesting is carried out 30-60 minute softening and slaking, obtain the mixture of softening and slaking at 60-80 ℃;
Can judge to discongest to soften whether carry out fully by detection fibers broken resistance intensity method with slaking; The weight ratio of described fabric softening and maize straw is 1-2: 100, and the weight ratio of described cationic surfactant and maize straw is 1-2: 100, and the mixture solid concentration of discongesting that control is used for fiber softening and maturation stage is 35-40wt%; Described cationic surfactant can be cationic surfactants such as fatty amine salt, ethanolamine salt, polyethylene polyamines salt and quaternary ammonium salt; Described fabric softening can be string softening agents such as imidazoline.
3) above-mentioned mixture softening and slaking is ground separate, obtain the maize straw fibre stuff, and the solid concentration of controlling the mixture that is used for grinding the softening and slaking of separating step is 15-35wt%, wherein the mill process of separating uses high dense mill to carry out twice, be corase grind for the first time, fixedly the gap between mill and the rotary grinding disk is 2-3mm, motor speed is 2000 rev/mins, treat to stop when the maize straw mill is separated beating degree 20-25, be correct grinding for the second time, fixedly the gap between mill and the rotary grinding disk is 1-1.5mm, motor speed is 2000 rev/mins, treats to stop when the maize straw mill is separated beating degree 30-35, and diverting oneself and concentrate through the dilution of routine then obtains the maize straw fibre stuff that 30kg measures, according to the beating degree index as can be known, above-mentioned fibre stuff can be used for producing paper pulp or paper behaviour and makes.Use physical sedimentation and flocculation technique to reclaim lignin from the waste water that the preparation slurry obtains, the yield of lignin is 9kg, and content of lignin is approximately 100g as can be known from the 1kg maize straw, and the rate of recovery of present embodiment method lignin is 90%.
When the multienzyme compound formulation that present embodiment provides is prepared the 30kg fibre stuff from above-mentioned 100kg maize straw, reclaimed the lignin of 9kg, do not use the method for high-temperature high-pressure chemical boiling in the preparation process of slurry, the fibre stuff that obtains can reach production paper pulp or paper is grasped the requirement of making, and can be used for preparation and do not have chemical residual, environment friendly and pollution-free packing box or paper products.
Embodiment 3, utilize multienzyme compound formulation of the present invention to obtain fibre stuff from wheat straw
At the wheat straw of 100kg, the multienzyme compound formulation that present embodiment uses is prepared from for the water that the multienzyme complex by 0.85kg adds 43-50kg, and the prescription of described multienzyme complex comprises the component of following weight portion: 33 parts laccase; The zytase of 25-26 part; The pectase of 10-11 part; The amylase of 1-3 part; The lipase of 8-9 part; The tannase of 3-5 part; 0.1-1 the coenzyme of part; The stabilizing agent of 1-3 part; The diatomite of 3-10 part; Described stabilizing agent is made up of stabilizing agent A, stabilizing agent B and stabilizing agent C; Described stabilizing agent A accounts for 30% of stabilizer formula amount, by Ca
2+Form with polyvinylpyrrolidone; Described stabilizing agent B accounts for 50% of stabilizer formula amount, is made up of EDTA and dithiothreitol (DTT); Described stabilizing agent C accounts for 20% of stabilizer formula amount, is made up of polyvinylpyrrolidone and EDTA; Ca among the described stabilizing agent A
2+With the weight ratio of polyvinylpyrrolidone be 9: 1; The weight ratio of EDTA and dithiothreitol (DTT) is 1: 40 among the described stabilizing agent B; The weight ratio of polyvinylpyrrolidone and EDTA is 80: 1 among the described stabilizing agent C.Described coenzyme is made up of biotin in the B family vitamin and riboflavin, wherein the purity of biotin is 99.2%, the purity of riboflavin is 98.5%, can carry out suitable selection to biotin in the coenzyme and riboflavin proportion of composing according to the characteristic of the employed various enzymes of present embodiment, those skilled in the art can finish based on its rudimentary knowledge, and present embodiment is not done particular determination for concrete portfolio ratio.
Wherein the enzyme of laccase is lived and is 3000U/g, and the enzyme work of zytase is 2,000,000 U/g, and the enzyme work of pectase is 900,000 U/g, and diastatic enzyme work is 800,000 U/g, and the enzyme of lipase is lived and is 25000U/g, and the enzyme work of tannase is 200 tannase units/gram.
Multienzyme compound formulation preparation process is as follows:
1) the pH value with water transfers to 7.5-9, adds amylase, lipase, tannase successively and stirs adding stabilizing agent A, mixing;
2) add successively in the mixture that step 1) obtains after pectase, zytase, laccase stir, add formula ratio 60% coenzyme and stabilizing agent B, mixing left standstill 5-10 minute;
3) in step 2) add the coenzyme and the diatomite mixing of formula ratio 20% in the mixture that obtains, place and leave standstill packing storage after 5-36 hour under the 4-6 ℃ of condition;
4) before the multienzyme compound formulation uses 0.5-1 hour, the coenzyme and the stabilizing agent C of adding surplus in the mixture that step 3) obtains, mixing.
Above-mentioned multienzyme compound formulation and specific preparation method thereof make this multienzyme compound formulation stable preservation over a period to come, and still have good vigor in use.
Wheat straw described in the present embodiment has also carried out getting the raw materials ready before the fibre stuff in preparation, dedusting removal of impurities and brooming step process, and wherein said material preparation step is included as the process with the wheat straw segment, and described brooming step is included as wheat straw is processed into thread process.In the brooming wheat straw wheat straw is sprayed water, making the dry weight of wheat straw in the wheat straw behind the water spray is 50-60wt%.
Use the above-mentioned multienzyme compound formulation for preparing to prepare fibre stuff, comprising from above-mentioned 100kg wheat straw:
1) uses the liquid of discongesting that forms by described multienzyme compound formulation thin up at room temperature wheat straw to be carried out preimpregnation, make wheat straw be discongested liquid and soak into fully, obtain the preimpregnation mixture; Total restatement with the preimpregnation mixture, the content of described wheat straw is 25-30wt%, the pH value of regulating above-mentioned preimpregnation mixture then is to 7.5-11, in not being higher than 65 ℃ temperature range, described preimpregnation mixture carried out discongesting in 16-18 hour obtain discongesting wheat straw completely, promptly discongest mixture, discongest whether fully by the method judgement that detects inserted link insertion resistance size;
2) add fabric softening and cationic surfactant in the mixture simultaneously above-mentioned discongesting, the described mixture of discongesting is carried out 30-60 minute softening and slaking, obtain the mixture of softening and slaking at 60-80 ℃;
Can judge to discongest to soften whether carry out fully by the method for detection fibers broken resistance intensity with slaking; The weight ratio of described fabric softening and wheat straw is 1-2: 100, and the weight ratio of described cationic surfactant and wheat straw is 1-2: 100, and the mixture solid concentration of discongesting that control is used for fiber softening and maturation stage is 35-40wt%; Described cationic surfactant can be cationic surfactants such as fatty amine salt, ethanolamine salt, polyethylene polyamines salt and quaternary ammonium salt; Described fabric softening can be string softening agents such as imidazoline;
3) above-mentioned mixture softening and slaking is ground separate, obtain the wheat straw fiber slurry, and the solid concentration of controlling the mixture that is used for grinding the softening and slaking of separating step is 15-35wt%, wherein the mill process of separating uses high dense mill to carry out twice, be corase grind for the first time, fixedly the gap between mill and the rotary grinding disk is 2-3mm, motor speed is 2000 rev/mins, treat to stop when the wheat straw mill is separated beating degree 20-25, be correct grinding for the second time, fixedly the gap between mill and the rotary grinding disk is 1-1.5mm, motor speed is 2000 rev/mins, treats to stop when the wheat straw mill is separated beating degree 30-35, and diverting oneself and concentrate through the dilution of routine then obtains the wheat straw fiber slurry that 74kg measures, according to the beating degree index as can be known, above-mentioned fibre stuff can be used for producing paper pulp or paper behaviour and makes.Use physical sedimentation and flocculation technique to reclaim lignin from the waste water that the preparation slurry obtains, the yield of lignin is 16kg, and content of lignin is approximately 250g as can be known from the 1kg wheat straw, and the rate of recovery of present embodiment method lignin is 64%.
When the multienzyme compound formulation that present embodiment provides is prepared the 74kg fibre stuff from above-mentioned 100kg wheat straw, reclaimed the lignin of 16kg, do not use the method for high-temperature high-pressure chemical boiling in the preparation process of slurry, the fibre stuff that obtains can reach production paper pulp or paper is grasped the requirement of making, and can be used for preparation and do not have chemical residual, environment friendly and pollution-free packing box or paper products.
Claims (10)
1. a multienzyme compound formulation is characterized in that, it is formulated to add water by multienzyme complex, and wherein the weight ratio of multienzyme complex and water is 1: 50-100, and the prescription of described multienzyme complex comprises the component of following weight portion: the laccase of 30-35 part; The zytase of 25-35 part; The pectase of 10-20 part; 0.1-10 the amylase of part; 0.3-10 the lipase of part; 0.1-5 the tannase of part; 0.1-3 the coenzyme of part; 0.1-3 the stabilizing agent of part; 0.3-25 the diatomite of part;
Described stabilizing agent is made up of stabilizing agent A, stabilizing agent B and stabilizing agent C;
Described stabilizing agent A accounts for 30% of stabilizer formula amount, by Ca
2+Form with polyvinylpyrrolidone;
Described stabilizing agent B accounts for 50% of stabilizer formula amount, is made up of EDTA and dithiothreitol (DTT);
Described stabilizing agent C accounts for 20% of stabilizer formula amount, is made up of polyvinylpyrrolidone and EDTA.
2. multienzyme compound formulation according to claim 1 is characterized in that, Ca among the described stabilizing agent A
2+With the weight ratio of polyvinylpyrrolidone be 8-10: 1;
The weight ratio of EDTA and dithiothreitol (DTT) is 1 among the described stabilizing agent B: 30-50;
The weight ratio of polyvinylpyrrolidone and EDTA is 70-90 among the described stabilizing agent C: 1.
3. multienzyme compound formulation according to claim 1 is characterized in that, described coenzyme is made up of in nicotinic acid, folic acid, biotin or the riboflavin one or more.
4. the preparation method of each described multienzyme compound formulation of claim 1-3 comprises:
1) the pH value with water transfers to 7.5-9, adds amylase, lipase, tannase and stirs adding stabilizing agent A, mixing;
2) add in the mixture that step 1) obtains after pectase, zytase, laccase stir, add the coenzyme and the stabilizing agent B of formula ratio 60%, mixing left standstill 5-10 minute;
3) in step 2) add the coenzyme and the diatomite mixing of formula ratio 20% in the mixture that obtains, place and leave standstill packing storage after 5-36 hour under the 4-6 ℃ of condition;
4) before the multienzyme compound formulation uses 0.5-1 hour, the coenzyme and the stabilizing agent C of adding surplus in the mixture that step 3) obtains, mixing.
5. the application of each described multienzyme compound formulation of claim 1-3 in utilizing agricultural wastes acquisition fibre stuff.
6. utilize the method for each described multienzyme compound formulation of claim 1-3, may further comprise the steps from agricultural wastes acquisition fibre stuff:
1) use the liquid of discongesting that forms by described multienzyme compound formulation thin up at room temperature agricultural wastes to be carried out preimpregnation and obtain the preimpregnation mixture, wherein saidly discongest that the weight ratio of multienzyme complex and described agricultural wastes is 0.7-1 in the liquid: 100, and with total restatement of described preimpregnation mixture, the content of described agricultural wastes is 25-30wt%; The pH value of regulating above-mentioned preimpregnation mixture is to 7.5-11, in not being higher than 65 ℃ temperature range, described preimpregnation mixture carried out at least 6 hours discongesting, and obtains discongesting mixture;
2) add fabric softening and cationic surfactant in the mixture above-mentioned discongesting, the described mixture of discongesting is carried out 30-60 minute softening and slaking, obtain the mixture of softening and slaking at 60-80 ℃;
3) above-mentioned mixture softening and slaking is ground separate, obtain the fibre stuff of agricultural wastes.
7. method according to claim 6, it is characterized in that, described agricultural wastes for through get the raw materials ready, the agricultural wastes of dedusting removal of impurities and brooming step process, wherein said material preparation step comprises the process with the agricultural wastes segment, and described brooming step comprises agricultural wastes are processed into thread process.
8. method according to claim 6 is characterized in that, the weight ratio of described fabric softening and described agricultural wastes is 1-2: 100, and the weight ratio of described cationic surfactant and described agricultural wastes is 1-2: 100.
9. according to each described method of claim 6-8, it is characterized in that, be used for softening and the concentration of discongesting the mixture solid content of slaking is 35-40wt%; The concentration that is used for grinding the mixture solid content of the softening and slaking of separating is 15-35wt%.
10. fibre stuff, described fibre stuff is prepared from according to each described method of claim 6-9.
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| CN106049186A (en) * | 2016-05-25 | 2016-10-26 | 安徽省丰蓼农业开发有限公司 | Manufacturing method of waterproof cardboard for packaging and transportation of melons, fruits and vegetables |
| CN106191021A (en) * | 2016-08-02 | 2016-12-07 | 长兴锦龙纸业有限公司 | A kind of ryegrass pulping complex enzyme liquid and application thereof |
| CN106222153A (en) * | 2016-08-02 | 2016-12-14 | 长兴锦龙纸业有限公司 | A kind of employing straw slurry complex enzyme liquid and application thereof |
| CN106244573A (en) * | 2016-08-02 | 2016-12-21 | 长兴锦龙纸业有限公司 | A kind of corn straw slurrying complex enzyme liquid and application thereof |
| CN114182361A (en) * | 2021-11-08 | 2022-03-15 | 宜兴市舜昌亚麻纺织有限公司 | Composite biological enzyme degumming agent suitable for flax fiber supercritical degumming |
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