CN101418289A - Trichoderma reesei liquid submerged fermentation cellulase and enzymatic beating process thereof - Google Patents
Trichoderma reesei liquid submerged fermentation cellulase and enzymatic beating process thereof Download PDFInfo
- Publication number
- CN101418289A CN101418289A CNA2008101536449A CN200810153644A CN101418289A CN 101418289 A CN101418289 A CN 101418289A CN A2008101536449 A CNA2008101536449 A CN A2008101536449A CN 200810153644 A CN200810153644 A CN 200810153644A CN 101418289 A CN101418289 A CN 101418289A
- Authority
- CN
- China
- Prior art keywords
- liquid
- fermentation
- cellulase
- enzyme
- pulp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a submerged fermentation cellulase of trichoderma reesei liquid and an enzymatic pulping process which is a novel process to use the trichoderma reesei as a fermentation strain to produce a cellulase used by self for the enzymatic pulping in a mode of deep liquid fermentation. The technical process is as follows: adopting paper pulp to be processed as a fermentation carbon source; and through the mode of the deep liquid fermentation, preparing the needed cellulase used for the enzymatic pumping after the optimization of a fermentation medium and a fermentation condition. The invention has the advantages of simple process, less equipment investment, low cost and compact technical process; the adoption of the pepper pulp to be processed as the fermentation carbon source can significantly improve the adaptability of an enzyme liquid; and the cellulase has high enzyme activity, less dosage and good effect, and can reduce the energy consumption of the pumping. The submerged fermentation cellulase is suitable for the pulping process of pulping raw materials under the prior structural condition of raw materials for paper making in China.
Description
Technical field
The present invention relates to a kind of cellulase enzymatic beating process, relate in particular to a kind of trichoderma reesei liquid submerged fermentation cellulase and enzymatic beating process thereof.
Background technology
Making beating is the important step of pulping and papermaking processes, to the physicals generation effect of paper web.Yet making beating is a power consumption workshop section.In order to obtain needed pulp properties, making beating or defibrination will consume a large amount of energy, account for greatly from former to expect into 18% of the required total energy of paper.Therefore, any pulp processing method that can significantly reduce making beating or refining energy consumption will produce useful influence to whole energy input.
PRATIMA BAJPAI is published in studies show that in " TAPPI Journal " 2006 the 5th volumes 11 phase 25-32 pages or leaves " Use of enzymes for reduction in refining energy-laboratory studies " literary composition: the FiberZyme LBR cellulase of the Biorefine L cellulase of India Biocon company and U.S. Dyadic international corporation can advantageously reduce beating energy consumption, and the strength property of paper pulp is not affected.
Ju Chengmin is published in and has introduced the plain enzyme of Novi such as N613, N476, N342, HC, D401 and D501 letter commercial fibre and hemicellulase in " Chinese papermaking " 2006 the 25th volume 1 phase 71-72 page or leaf " biological enzyme is not to floating the influence of needlebush beatability " literary composition and improving beatability and improving influence aspect the pulp strength.
The cellulase that above-mentioned institute uses is a commercial enzyme all, is not to make cellulase by oneself according to practical situation to be used for enzymatic beating.Because the height of the plain enzyme price comparison of commercial fibre now, it is very high to make factory use cost, domestic in addition paper making raw material shortage, the fiber of most of fast growing wood such as needlebushs such as Pinus massoniana Lamb, tamarack and pinus khasys is not only thick but also long, its sulfate pulp is difficult to making beating, make a little less than the market competitiveness of commercial pulp, deficiency in economic performance, for this reason, the paper industry demands developing the particular case according to factory urgently, adopt homemade on the spot cellulose enzyme liquid to carry out enzymatic beating process, to reach the effect that reduces production costs with beating energy consumption.
Summary of the invention
The object of the present invention is to provide a kind of trichoderma reesei liquid submerged fermentation cellulase and enzymatic beating process thereof, use trichoderma reesei liquid submerged fermentation cellulase that the fast growing wood sulfate pulp is carried out enzymatic beating, can reduce beating energy consumption and production cost.
The present invention for achieving the above object, the technical scheme of employing:
A kind of cellulase is characterized in that: described cellulase, and the production bacterial strain that it uses is Trichodermareesei, preserving number, Trichodermareesei is an aerobic bacteria, growing environment is extensive, adaptability is stronger, be easy to control, be convenient to management, its metabolite safety non-toxic, can not influence producers and environment, the good stability of institute's cellulase-producing should not be degenerated, and preparation method of cellulase is as follows:
Obtain needed liquid seeds with carrying out seed fermentation in the Trichodermareesei test tube slant bacterial classification access liquid seeds fermentor tank; Then according to certain inoculum size with the plain enzymic fermentation jar of liquid seeds incoming fiber, and, make required cellulose enzyme liquid through centrifugation at last, and it be stored in the cellulase storage tank with standby at certain fermentation condition bottom fermentation,
Its fermentation parameter is as follows:
1), Trichodermareesei test tube slant substratum: 1%-2%CMC, 4%-8% nutritive element liquid, 0.1%-0.5% liquid microelement, 2%-3% agar, pH6.0;
2), the cultivation of Trichodermareesei bacterial classification: bacterial classification is connected on the slant medium, in incubator, after cultivating 4-7 days under 29 ± 1 ℃ of conditions, use or 4 ℃ preserve standby down;
3), liquid seeds fermention medium: 1%-3% glucose, 4%-8% nutritive element liquid, 1%-4% wheat bran, 0.1%-0.4% liquid microelement;
4), cellulase fermentations substratum: carbon source is a 0.1%-3% fast growing wood sulfate pulp, 8%-10% nutritive element liquid, 0.2%-0.5% liquid microelement, 1%-4% wheat bran, 0.1%-0.2% tensio-active agent Tween-80;
5), liquid seeds fermentation condition: temperature 30-40 ℃, pH5-7, stirring velocity 250-300r/min, ventilation 0.2-0.3m
3/ h, fermentation time 36-48h;
6), cellulase fermentations condition: temperature 30-40 ℃, pH5-8, inoculum size 2%-3%, stirring velocity 250-300r/min, ventilation 0.2-0.3m
3/ h, fermentation time 72-96h;
Described its prescription of nutritive element liquid is: get the 3-10g Trisodium Citrate, and the 5-20g potassium primary phosphate, 1-10g ammonium sulfate, 0.5-5g sal epsom is settled to 100ml after the dissolving of 0.1-5g calcium chloride;
Described micro-liquid formula is: the 1-5g ferrous sulfate, and the 0.1-1g manganous sulfate, the 0.1-1g zinc chloride, the 0.5-5g cobalt chloride, 1-10ml 2N HCl dissolving is fixed molten to 100ml; Above-mentioned all per-cents all by weight.
In preparation method of cellulase of the present invention, used fermenting carbon source is the pinus khasys sulfate pulp.
In preparation method of cellulase of the present invention, in the preparation process of its enzyme liquid, the seed fermentation substratum adopts: 1% glucose, 4% nutritive element liquid, 1% wheat bran, 0.1% liquid microelement is 30 ℃ of temperature, pH is 6, stirring velocity 250r/min, ventilation 0.2m
3The condition bottom fermentation 48h of/h obtains needed liquid seeds; According to 2% inoculum size, with the plain enzymic fermentation jar of seed incoming fiber, fermention medium adopts: carbon source is 0.1% fast growing wood sulfate pulp then, 8% nutritive element liquid, 0.2% liquid microelement, 1% wheat bran, 0.2% tensio-active agent Tween-80 is at 30 ℃ of temperature, pH6; Inoculum size 2%, stirring velocity 300r/min; Ventilation 0.3m
3The condition bottom fermentation 96h of/h.
Performance with the prepared cellulase product of preparation method of cellulase of the present invention is:
The enzyme work of cellulose enzyme liquid is respectively: FPA enzyme 900-1500u/ml alive, Cx enzyme 3000-4000u/ml alive, Cb enzyme live and are 10-100u/ml, Cl enzyme 500-1000u/ml alive.
The cellulase that adopts the present invention's invention to make is used for enzymatic beating, and its beating process is:
Paper pulp is the fast growing wood sulfate pulp, prepare cellulase according to the method described in the present invention and it is stored in the cellulase storage tank with standby, is unit with the enzyme dosage of 3-9u/g with the work of FPA enzyme by volume pump with the enzyme liquid in the cellulase storage tank, be transported to the paper pulp reaction tank paper pulp is carried out pre-treatment, treatment condition are:
Starching dense is 3%-10%, temperature: 40-60 ℃, pH value: 6-8, treatment time: 1-2h, and the paper pulp after cellulose treatment is transported to disc refiner and pulls an oar.
In beating process of the present invention, described fast growing wood sulfate pulp is the pinus khasys sulfate pulp, and enzyme dosage enzyme dosage 7u/g is a unit with the work of FPA enzyme, with dense 10%, and 50 ℃ of temperature, pH is 7.8 (pH of paper pulp own), treatment time 1h.
The invention has the beneficial effects as follows:
1. technology is simple, and production cost is low.
2. adopt paper pulp to be processed be the adaptability that fermenting carbon source can significantly improve enzyme liquid.
3. the work of the enzyme of cellulase is high, consumption is few, with strong points, effective.
4. the reduction beating energy consumption is little to the damage of fiber itself.
Description of drawings
Fig. 1 is a cellulase enzymatic beating process schematic flow sheet of the present invention.
Embodiment
Below in conjunction with drawings and Examples to the detailed description of the invention.
Referring to Fig. 1, a kind of preparation of cellulase, the production bacterial strain that it uses is Trichodermareesei.Obtain needed liquid seeds with carrying out seed fermentation in the Trichodermareesei test tube slant bacterial classification access liquid seeds fermentor tank 1; Then according to certain inoculum size with the plain enzymic fermentation jar 2 of liquid seeds incoming fiber, and, make required cellulose enzyme liquid through centrifugation at last, and it be stored in the cellulase storage tank 3 with standby at certain fermentation condition bottom fermentation,
The preparation of enzyme liquid: Trichodermareesei test tube slant bacterial classification is inserted in the liquid seeds fermentor tank 1, the seed fermentation substratum adopts: 1% glucose, 4% nutritive element liquid, 1% wheat bran and 0.1% liquid microelement, and at 30 ℃ of temperature, pH5.76, stirring velocity 250r/min, ventilation 0.2m
3The condition bottom fermentation 48h of/h obtains needed liquid seeds; Then according to 2% inoculum size with the plain enzymic fermentation jar 2 of seed incoming fiber; Fermention medium adopts: 0.1% pinus khasys sulfate pulp, 8% nutritive element liquid, 0.2% liquid microelement, 1% wheat bran and tensio-active agent Tween-80 add-on 0.2%, and at 30 ℃ of temperature, pH6, stirring velocity 300r/min, ventilation 0.2m
3The condition bottom fermentation 96h of/h makes required cellulose enzyme liquid through centrifugation then, and it is stored in the cellulase storage tank 3 with standby.The enzyme work of made cellulose enzyme liquid is respectively: FPA enzyme 981.67u/ml alive, and Cx enzyme 3083.19u/ml alive, the work of Cb enzyme is 27.79, Cl enzyme 571.42u/ml alive.
Enzymatic beating process:
Paper pulp is the pinus khasys sulfate pulp, is unit with the enzyme dosage of 7u/g with the work of FPA enzyme by volume pump with the enzyme liquid in the cellulase storage tank 3, be transported to 4 pairs of paper pulp of paper pulp reaction tank and carry out pre-treatment, treatment condition are: starch dense 10%, 50 ℃ of temperature, the pH value is 7.8 (the pH values of paper pulp own), treatment time 1h, and the paper pulp after cellulase is handled is transported to disc refiner 5 and pulls an oar.
According to starching sample: the blank paste sample.
Prepare the blank paste sample by those skilled in the art of the present technique's known method, promptly do not use cellulase of the present invention that paper pulp is pulled an oar.
According to starching sample: the plain enzyme enzymatic beating slurry of commercial fibre sample.
Under the same treatment condition, adopt the plain enzyme of commercial fibre to carry out enzymatic beating.
According to national paper pulp test method for physical property, measure the present invention's slurry sample of pulling an oar, the physicals of the plain enzyme making beating of blank paste sample and commercial fibre slurry sample sees Table 1.
Table 1
As seen from Table 1, enzyme of the present invention is handled the slurry sample can obviously reduce the paste roller mill load, thereby saves refining energy consumption, energy-conservation about 25%, suitable with the energy-conservation value of the making beating of commercial fibre enzyme, and the physicals of paper pulp is not subjected to too big influence.
Adopt trichoderma reesei fermented cellulosic enzyme to carry out fast growing wood NBSK enzymatic beating, technology is simple, Equipment investment is few, cost is low, technological process is compact, has the effect that reduces beating energy consumption, is fit to use Beating process in the pulping raw material of the existing papermaking material structure situation of China.
Claims (6)
1, a kind of preparation method of cellulase is characterized in that: described cellulase, and the production bacterial strain that it uses is Trichodermareesei, preserving number is that described preparation method of cellulase is as follows:
Obtain needed liquid seeds with carrying out seed fermentation in the Trichodermareesei test tube slant bacterial classification access liquid seeds fermentor tank (1), then according to certain inoculum size with the plain enzymic fermentation jar (2) of liquid seeds incoming fiber, and at certain technological condition for fermentation bottom fermentation, make required cellulose enzyme liquid through centrifugation at last, and it is stored in the cellulase storage tank (3) with standby
Described fermentation parameter is as follows:
1), Trichodermareesei test tube slant substratum: 1%-2%CMC, 4%-8% nutritive element liquid, 0.1%-0.5% liquid microelement, 2%-3% agar, pH6.0;
2), the cultivation of Trichodermareesei bacterial classification: bacterial classification is connected on the slant medium, in incubator, after cultivating 4-7 days under 29 ± 1 ℃ of conditions, use or 4 ℃ preserve standby down;
3), liquid seeds fermention medium: 1%-3% glucose, 4%-8% nutritive element liquid, 1%-4% wheat bran, 0.1%-0.4% liquid microelement;
4), cellulase fermentations substratum: carbon source is a 0.1%-3% fast growing wood sulfate pulp, 8%-10% nutritive element liquid, 0.2%-0.5% liquid microelement, 1%-4% wheat bran, 0.1%-0.2% tensio-active agent Tween-80;
5), liquid seeds fermentation condition: temperature 30-40 ℃, pH5-7, stirring velocity 250-300r/min, ventilation 0.2-0.3m
3/ h, fermentation time 36-48h;
6), cellulase fermentations condition: temperature 30-40 ℃, pH5-8, inoculum size 2%-3%, stirring velocity 250-300r/min, ventilation 0.2-0.3m
3/ h, fermentation time 72-96h;
Described nutritive element liquid formula is: get the 3-10g Trisodium Citrate, and the 5-20g potassium primary phosphate, 1-10g ammonium sulfate, 0.5-5g sal epsom is settled to 100ml after the dissolving of 0.1-5g calcium chloride;
Described micro-liquid formula is: the 1-5g ferrous sulfate, and the 0.1-1g manganous sulfate, the 0.1-1g zinc chloride, the 0.5-5g cobalt chloride, 1-10ml 2N HCl dissolving is fixed molten to 100ml; Above-mentioned all per-cents all by weight.
2. the described method of claim 1, it is characterized in that: described carbon source is the pinus khasys sulfate pulp.
3. the described method of claim 1 is characterized in that: in the preparation process of enzyme liquid, the seed fermentation substratum adopts: 1% glucose, 4% nutritive element liquid, 1% wheat bran, 0.1% liquid microelement is 30 ℃ of temperature, pH is 6, stirring velocity 250r/min, ventilation 0.2m
3The condition bottom fermentation 48h of/h obtains needed liquid seeds; According to 2% inoculum size, with the plain enzymic fermentation jar (2) of seed incoming fiber, fermention medium adopts: carbon source is 0.1% fast growing wood sulfate pulp then, 8% nutritive element liquid, 0.2% liquid microelement, 1% wheat bran, 0.2% tensio-active agent Tween-80 is at 30 ℃ of temperature, pH6; Inoculum size 2%, stirring velocity 300r/min; Ventilation 0.3m
3The condition bottom fermentation 96h of/h.
4. the described method of claim 1, it is characterized in that: the performance of prepared cellulase product is that the enzyme work of cellulose enzyme liquid is respectively: FPA enzyme 900-1500u/ml alive, Cx enzyme 3000-4000u/ml alive, Cb enzyme live and are 10-100u/ml, C1 enzyme 500-1000u/ml alive.
5, a kind of cellulase that adopts the described method of claim 1 to make is used for the technology of enzymatic beating, it is characterized in that: paper pulp is the fast growing wood sulfate pulp, prepare cellulase in accordance with the method for claim 1 and it is stored in the cellulase storage tank with standby, is unit with the enzyme dosage of 3-9u/g with the work of FPA enzyme by volume pump with the enzyme liquid in the cellulase storage tank (3), be transported to paper pulp reaction tank (4) paper pulp is carried out pre-treatment, treatment condition are: starch dense 3%-10%, temperature 40-60 ℃, pH6-8, treatment time 1-2h, the paper pulp after cellulose treatment are transported to disc refiner (5) and pull an oar.
6. the described beating process of claim 5, it is characterized in that: described fast growing wood sulfate pulp is the pinus khasys sulfate pulp, enzyme dosage 7u/g is a unit with the work of FPA enzyme, starch dense 10%, 50 ℃ of temperature, pH is 7.8 (pH of paper pulp own), treatment time 1h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101536449A CN101418289B (en) | 2008-11-28 | 2008-11-28 | Trichoderma reesei liquid submerged fermentation cellulase and enzymatic beating process thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101536449A CN101418289B (en) | 2008-11-28 | 2008-11-28 | Trichoderma reesei liquid submerged fermentation cellulase and enzymatic beating process thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101418289A true CN101418289A (en) | 2009-04-29 |
| CN101418289B CN101418289B (en) | 2011-05-04 |
Family
ID=40629292
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008101536449A Expired - Fee Related CN101418289B (en) | 2008-11-28 | 2008-11-28 | Trichoderma reesei liquid submerged fermentation cellulase and enzymatic beating process thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101418289B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102392006A (en) * | 2011-11-29 | 2012-03-28 | 天津工业生物技术研究所 | Production method for raising output of acidic cellulose produced by use of Trichoderma spp |
| CN101671625B (en) * | 2009-09-28 | 2012-08-15 | 厦门北大泰普科技有限公司 | Method and device for preparing trichoderma conidium by liquid state submerged fermentation |
| CN101654669B (en) * | 2009-09-02 | 2012-11-21 | 安徽丰原发酵技术工程研究有限公司 | Method for producing cellulase in high yield |
| CN103509770A (en) * | 2012-06-26 | 2014-01-15 | 湖南鸿鹰祥生物工程股份有限公司 | Method for producing cellulase through fermentation by adopting corn cob as main raw material |
| CN105442371A (en) * | 2015-10-29 | 2016-03-30 | 天津市昌维生物科技有限公司 | Energy-saving and cost-reducing agent for paper pulp |
| CN109810964A (en) * | 2017-11-21 | 2019-05-28 | 湖南海正生物科技有限公司 | A kind of mixing enzyme preparation and preparation method thereof for mashing |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103509721B (en) * | 2012-06-26 | 2017-09-22 | 湖南鸿鹰祥生物工程股份有限公司 | The method of one plant height cellulase producing strain and fermentation production neutral cellulase |
| CN103740680B (en) * | 2013-12-23 | 2016-04-20 | 湖南鸿鹰生物科技有限公司 | The method of Trichodermareesei fermentative production cellulase and bacterial strain application thereof |
| CN103740678B (en) * | 2013-12-23 | 2016-08-17 | 湖南鸿鹰生物科技有限公司 | Cellulase and preparation thereof |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9018426D0 (en) * | 1990-08-22 | 1990-10-03 | Sandoz Ltd | Improvements in or relating to novel compounds |
| CN1185336C (en) * | 2002-04-19 | 2005-01-19 | 浙江大学 | Li's Trichoderma strains and use thereof |
| CN100497647C (en) * | 2006-01-18 | 2009-06-10 | 中国科学院过程工程研究所 | Method for adsorbing and decomposing stalk cellulose using cellulase |
| CN101250511B (en) * | 2008-04-16 | 2010-12-01 | 中国石油化工股份有限公司 | Method for promoting cellulolytic enzymes synthesis of trichoderma reesei by using dilute sulfuric acid treated pulp as carbon source |
| CN101250485B (en) * | 2008-04-16 | 2012-02-01 | 中国石油化工股份有限公司 | Trichoderma reesei cultivation method for improving yield of cellulase |
-
2008
- 2008-11-28 CN CN2008101536449A patent/CN101418289B/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101654669B (en) * | 2009-09-02 | 2012-11-21 | 安徽丰原发酵技术工程研究有限公司 | Method for producing cellulase in high yield |
| CN101671625B (en) * | 2009-09-28 | 2012-08-15 | 厦门北大泰普科技有限公司 | Method and device for preparing trichoderma conidium by liquid state submerged fermentation |
| CN102392006A (en) * | 2011-11-29 | 2012-03-28 | 天津工业生物技术研究所 | Production method for raising output of acidic cellulose produced by use of Trichoderma spp |
| CN103509770A (en) * | 2012-06-26 | 2014-01-15 | 湖南鸿鹰祥生物工程股份有限公司 | Method for producing cellulase through fermentation by adopting corn cob as main raw material |
| CN105442371A (en) * | 2015-10-29 | 2016-03-30 | 天津市昌维生物科技有限公司 | Energy-saving and cost-reducing agent for paper pulp |
| CN109810964A (en) * | 2017-11-21 | 2019-05-28 | 湖南海正生物科技有限公司 | A kind of mixing enzyme preparation and preparation method thereof for mashing |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101418289B (en) | 2011-05-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101418289B (en) | Trichoderma reesei liquid submerged fermentation cellulase and enzymatic beating process thereof | |
| Song et al. | Production and characterization of cellulases and xylanases of Cellulosimicrobium cellulans grown in pretreated and extracted bagasse and minimal nutrient medium M9 | |
| Kalogeris et al. | Performance of an intermittent agitation rotating drum type bioreactor for solid-state fermentation of wheat straw | |
| Díaz et al. | Enhance hydrolytic enzymes production by Aspergillus awamori on supplemented grape pomace | |
| Updegraff | Utilization of cellulose from waste paper by Myrothecium verrucaria | |
| Bailey et al. | Production of xylanolytic enzymes by strains of Aspergillus | |
| Shah et al. | Xylanase production under solid-state fermentation and its characterization by an isolated strain of Aspergillus foetidus in India | |
| Bailey et al. | Effect of pH on production of xylanase by Trichoderma reesei on xylan-and cellulose-based media | |
| Szendefy et al. | Potential of solid-state fermentation enzymes of Aspergillus oryzae in biobleaching of paper pulp | |
| Damaso et al. | Use of corncob for endoxylanase production by thermophilic fungus Thermomyces lanuginosus IOC-4145 | |
| CN100588712C (en) | Method for preparing ethanol by using material conataining cassava residues | |
| Michelin et al. | Production and properties of xylanases from Aspergillus terricola Marchal and Aspergillus ochraceus and their use in cellulose pulp bleaching | |
| Liu et al. | Consolidated bioprocess for bioethanol production with alkali-pretreated sugarcane bagasse | |
| EP2703493A1 (en) | Detoxification with reducing agents | |
| Nair et al. | Purification and biochemical characterization of two xylanases from Aspergillus sydowii SBS 45 | |
| Romdhane et al. | Improvement of highly thermostable xylanases production by Talaromyces thermophilus for the agro-industrials residue hydrolysis | |
| Wang et al. | Production of cellulase from kraft paper mill sludge by Trichoderma reesei rut C-30 | |
| CN101423848A (en) | Method for preparing ethanol by using raw material containing manioc waste | |
| Dos Reis et al. | Cellulase and xylanase expression in response to different pH levels of Penicillium echinulatum S1M29 medium | |
| CN104694587B (en) | A kind of method that lactic acid is produced by bagasse | |
| CN109825487A (en) | A kind of industrial fermentation process of co-producing xylanase and other coenzyme | |
| Shah et al. | Optimization of cellulase production by Penicillium oxalicum using banana agrowaste as a substrate | |
| Abdeshahian et al. | Solid substrate fermentation for cellulase production using palm kernel cake as a renewable lignocellulosic source in packed-bed bioreactor | |
| CN104872378A (en) | Method for preparing high-protein feed by using baijiu vinasse | |
| WO2018159573A1 (en) | Method for producing saccharifying enzyme and method for oligosaccharide production |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110504 Termination date: 20171128 |