CN109825487A - A kind of industrial fermentation process of co-producing xylanase and other coenzyme - Google Patents
A kind of industrial fermentation process of co-producing xylanase and other coenzyme Download PDFInfo
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- CN109825487A CN109825487A CN201711183921.6A CN201711183921A CN109825487A CN 109825487 A CN109825487 A CN 109825487A CN 201711183921 A CN201711183921 A CN 201711183921A CN 109825487 A CN109825487 A CN 109825487A
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Abstract
A kind of industrial fermentation process of co-producing xylanase and other coenzyme, aspergillus niger is inoculated in seed culture medium in eggplant bottle to cultivate, 28 DEG C -35 DEG C of cultivation temperature, time is for 24 hours, as fermentation seed liquid, it is inoculated into seeding tank, seed culture is carried out with fermentation medium, 28 DEG C -35 DEG C of fermentation temperature, fermented and cultured 30h-36h, seed liquor is collected after fermented and cultured, it is seeded in fermentor, carries out fermented and cultured, fermentation ends with fermentation medium, fermentation liquid in collecting tank obtains zytase and other coenzyme.Advantage is: carrying out fermentation with aspergillus niger and prepares zytase, rational technology, zymotechnique is stablized, product viable count is high, enzyme activity is high, market application prospect is wide, and coenzyme includes neutral proteinase, alkali protease, acid protease, fungal amylase, cellulase, 1,4 beta-glucanase, pectase, carbohydrase, the sweet mannase of neutral mannonase acid, is suitble to industrialized production.
Description
Technical field
The present invention relates to the industrial fermentation process of a kind of co-producing xylanase and other coenzyme.
Background technique
Zytase belongs to hydrolase, is the enzyme of single-minded degradation of xylan, can be wood oligose by xylanolitic.Wood is poly-
Carbohydrase is the main component of plant hemicellulose, is present in plant cell wall, and the 15%-35% of plant cell dry weight is accounted for.Greatly
Most xylans be it is a kind of it is complicated, with highly branched heterogeneous polysaccharide, containing there are many different substituent groups.
Zytase suffers from extremely extensive purposes in industries such as feed, food, weaving, papermaking and daily-use chemical industries.It can
Be added in feed, can promote the clarification of fruit juice and beer, can be used as food additives, in baked goods replace lecithin and
Improve the quality of baked good；It can be used for paper industry without chlorine bio-pulping and pre-bleaching, the brightness of paper can be increased without shadow
The intensity of paper is rung, market capacity is very big.The producer of domestic production zytase is less at present, and enzyme activity unit is lower, quality
It is unstable.
Summary of the invention
The technical problem to be solved in the present invention is to provide the industrial fermentation process of a kind of co-producing xylanase and other coenzyme,
Rational technology, low in cost, product viable count is high, and enzymatic activity is high, and coenzyme type is more, is suitble to industrialized production.
The technical solution of the invention is as follows:
A kind of industrial fermentation process of co-producing xylanase and other coenzyme, the specific steps of which are as follows:
(1) culture medium forms
Seed culture medium, 1L: glucose 2-6%, peptone 1-1.5%, wheat bran 2-5%, defoaming agent 0.03%, excess water, pH
Value 5.5；
Solid fermentation culture medium, 1L: wheat bran 90%, ammonium sulfate 1%, glucose 3-5%, Tween-80 0.08%, excess water；
(2) triangular flask culture
Aspergillus niger is inoculated in seed culture medium in eggplant bottle to cultivate, inoculum concentration 3%-5%, condition of culture are as follows: temperature
28 DEG C -35 DEG C of degree, time are for 24 hours, as fermentation seed liquid；
(3) seed culture
Fermentation seed liquid is inoculated into seeding tank, carries out seed culture, inoculum concentration 3%-5%, fermentation with fermentation medium
28 DEG C -35 DEG C of temperature, tank presses 0.08Mpa, fermented and cultured 30h-36h, revolving speed 140r/min-160r/min, air quantity 60m3/ h, hair
Seed liquor is collected after ferment culture；
(4) fermented and cultured
Seed liquor is seeded in fermentor, carries out fermented and cultured with fermentation medium, inoculum concentration 3%-5% is controlled after inoculation
Water content 60vol% processed, fermentation temperature are 28 DEG C -35 DEG C, incubation time 100h-140h, fermentation ends, fermentation in collecting tank
Liquid obtains zytase and other coenzyme.
Beneficial effects of the present invention:
Fermentation, which is carried out, with Aspergillus niger (aspergillus niger) prepares zytase, optimized actication of culture condition, work of fermenting
Skill optimal control establishes stable industrialization fermentation manufacturing technique, and rational technology, product viable count is high, is sent out using this method
For the Xylanase activity prepared after ferment up to 8500u/mL, coenzyme is more, and vigor is high, and market application prospect is wide, and coenzyme includes neutrality
Protease, alkali protease, acid protease, fungal amylase, cellulase, 1,4 beta-glucanase, pectase, carbohydrase, in
Property the sweet mannase of mannonase acid, be suitble to industrialized production.
Specific embodiment
Embodiment 1
(1) culture medium forms
Seed culture medium, 1L: glucose 2%, peptone 1.5%, wheat bran 2%, defoaming agent 0.03%, excess water, pH value 5.5；
Solid fermentation culture medium, 1L: wheat bran 90%, ammonium sulfate 1%, glucose 3%, Tween-80 0.08%, excess water；
(2) triangular flask culture
Fermenting microbe: Aspergillus niger (aspergillus niger)；
Aspergillus niger is inoculated in seed culture medium in eggplant bottle to cultivate, inoculum concentration 3%, condition of culture are as follows: temperature 28
DEG C, the time is for 24 hours, as fermentation seed liquid；
(3) seed culture
Fermentation seed liquid is inoculated into seeding tank, carries out seed culture, inoculum concentration 3%, fermentation temperature with fermentation medium
28 DEG C, tank presses 0.08Mpa, fermented and cultured 30h, revolving speed 140r/min, air quantity 60m3/ h collects seed liquor after fermented and cultured；
(4) fermented and cultured
Seed liquor is seeded in fermentor, carries out fermented and cultured with fermentation medium, inoculum concentration 3%, control contains after inoculation
Water 60vol%, fermentation temperature are 28 DEG C, incubation time 100h, fermentation ends, and fermentation liquid in collecting tank obtains xylan
Enzyme and other coenzyme, coenzyme include neutral proteinase, alkali protease, acid protease, fungal amylase, cellulase, β-
Dextranase, pectase, carbohydrase, the sweet mannase of neutral mannonase acid.
Embodiment 2
(1) culture medium forms
Seed culture medium, 1L: glucose 6%, peptone 1%, wheat bran 5%, defoaming agent 0.03%, excess water, pH value 5.5；Gu
Body fermentation medium, 1L: wheat bran 90%, ammonium sulfate 1%, glucose 5%, Tween-80 0.08%, excess water；
(2) triangular flask culture
Aspergillus niger is inoculated in seed culture medium in eggplant bottle to cultivate, condition of culture are as follows: 35 DEG C of temperature, the time be for 24 hours,
As fermentation seed liquid；
(3) seed culture
Fermentation seed liquid is inoculated into seeding tank, carries out seed culture, inoculum concentration 5%, fermentation temperature with fermentation medium
35 DEG C, tank presses 0.08Mpa, fermented and cultured 36h, revolving speed 160r/min, air quantity 60m3/ h collects seed liquor after fermented and cultured；
(4) fermented and cultured
Seed liquor is seeded in fermentor, carries out fermented and cultured with fermentation medium, inoculum concentration 5%, control contains after inoculation
Water 60vol%, fermentation temperature are 35 DEG C, incubation time 140h, fermentation ends, and fermentation liquid in collecting tank obtains xylan
Enzyme and other coenzyme, coenzyme include neutral proteinase, alkali protease, acid protease, fungal amylase, cellulase, β-
Dextranase, pectase, carbohydrase, the sweet mannase of neutral mannonase acid.
Embodiment 3
(1) culture medium forms
Seed culture medium, 1L: glucose 4%, peptone 1.2%, wheat bran 3%, defoaming agent 0.03%, excess water, pH value 5.5；
Solid fermentation culture medium, 1L: wheat bran 90%, ammonium sulfate 1%, glucose 4%, Tween-80 0.08%, excess water；
(2) triangular flask culture
Aspergillus niger is inoculated in seed culture medium in eggplant bottle to cultivate, condition of culture are as follows: 32 DEG C of temperature, the time be for 24 hours,
As fermentation seed liquid；
(3) seed culture
Fermentation seed liquid is inoculated into seeding tank, carries out seed culture, inoculum concentration 4%, fermentation temperature with fermentation medium
30 DEG C, tank presses 0.08Mpa, fermented and cultured 32h, revolving speed 150r/min, air quantity 60m3/ h collects seed liquor after fermented and cultured；
(4) fermented and cultured
Seed liquor is seeded in fermentor, carries out fermented and cultured with fermentation medium, inoculum concentration 4%, control contains after inoculation
Water 60vol%, fermentation temperature are 30 DEG C, incubation time 120h, fermentation ends, and fermentation liquid in collecting tank obtains xylan
Enzyme and other coenzyme, coenzyme include neutral proteinase, alkali protease, acid protease, fungal amylase, cellulase, β-
Dextranase, pectase, carbohydrase, the sweet mannase of neutral mannonase acid.
The testing result of embodiment 3: zytase (national standard) 8500u/mL moisture 40%；
Other coenzyme:
The above is only specific embodiments of the present invention, are not intended to restrict the invention, for those skilled in the art
For member, the invention may be variously modified and varied.All within the spirits and principles of the present invention, it is made it is any modification,
Equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (1)
1. the industrial fermentation process of a kind of co-producing xylanase and other coenzyme, it is characterized in that:
Specific step is as follows:
(1) culture medium forms
Seed culture medium, 1L: glucose 2-6%, peptone 1-1.5%, wheat bran 2-5%, defoaming agent 0.03%, excess water, pH
Value 5.5；
Solid fermentation culture medium, 1L: wheat bran 90%, ammonium sulfate 1%, glucose 3-5%, Tween-80 0.08%, excess water；
(2) triangular flask culture
Aspergillus niger is inoculated in seed culture medium in eggplant bottle to cultivate, inoculum concentration 3%-5%, condition of culture are as follows: temperature
28 DEG C -35 DEG C of degree, time are for 24 hours, as fermentation seed liquid；
(3) seed culture
Fermentation seed liquid is inoculated into seeding tank, carries out seed culture, inoculum concentration 3%-5%, fermentation with fermentation medium
28 DEG C -35 DEG C of temperature, tank presses 0.08Mpa, fermented and cultured 30h-36h, revolving speed 140r/min-160r/min, air quantity 60m3/ h, hair
Seed liquor is collected after ferment culture；
(4) fermented and cultured
Seed liquor is seeded in fermentor, carries out fermented and cultured with fermentation medium, inoculum concentration 3%-5% is controlled after inoculation
Water content 60vol% processed, fermentation temperature are 28 DEG C -35 DEG C, incubation time 100h-140h, fermentation ends, fermentation in collecting tank
Liquid obtains zytase and other coenzyme.
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| CN201711183921.6A CN109825487A (en) | 2017-11-23 | 2017-11-23 | A kind of industrial fermentation process of co-producing xylanase and other coenzyme |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111019840A (en) * | 2019-12-30 | 2020-04-17 | 广东省农业科学院动物卫生研究所 | Method for producing cellulase, pectinase and xylanase by fermenting herbal tea residues by combining aspergillus niger and saccharomycetes |
| CN111593036A (en) * | 2020-06-08 | 2020-08-28 | 杭州保安康生物技术有限公司 | Preparation of enzyme preparation mainly containing acid protease, strain and application thereof |
| CN111607574A (en) * | 2020-06-08 | 2020-09-01 | 杭州保安康生物技术有限公司 | Enzyme preparation mainly comprising xylanase and acid protease, and strain and application thereof |
| CN111642639A (en) * | 2020-03-02 | 2020-09-11 | 漳州大北农农牧科技有限公司 | Piglet biological fermentation feed |
| WO2024037131A1 (en) * | 2022-08-15 | 2024-02-22 | 安琪酵母股份有限公司 | Organic solid-state fermentation enzyme formulation and organic plant protease hydrolysate, and preparation methods therefor |
-
2017
- 2017-11-23 CN CN201711183921.6A patent/CN109825487A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111019840A (en) * | 2019-12-30 | 2020-04-17 | 广东省农业科学院动物卫生研究所 | Method for producing cellulase, pectinase and xylanase by fermenting herbal tea residues by combining aspergillus niger and saccharomycetes |
| CN111642639A (en) * | 2020-03-02 | 2020-09-11 | 漳州大北农农牧科技有限公司 | Piglet biological fermentation feed |
| CN111593036A (en) * | 2020-06-08 | 2020-08-28 | 杭州保安康生物技术有限公司 | Preparation of enzyme preparation mainly containing acid protease, strain and application thereof |
| CN111607574A (en) * | 2020-06-08 | 2020-09-01 | 杭州保安康生物技术有限公司 | Enzyme preparation mainly comprising xylanase and acid protease, and strain and application thereof |
| CN111593036B (en) * | 2020-06-08 | 2023-01-20 | 杭州保安康生物技术有限公司 | Preparation of enzyme preparation mainly containing acid protease, strain and application thereof |
| WO2024037131A1 (en) * | 2022-08-15 | 2024-02-22 | 安琪酵母股份有限公司 | Organic solid-state fermentation enzyme formulation and organic plant protease hydrolysate, and preparation methods therefor |
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