CN107974404A - Enzyme digestion reaction kettle - Google Patents
Enzyme digestion reaction kettle Download PDFInfo
- Publication number
- CN107974404A CN107974404A CN201711044623.9A CN201711044623A CN107974404A CN 107974404 A CN107974404 A CN 107974404A CN 201711044623 A CN201711044623 A CN 201711044623A CN 107974404 A CN107974404 A CN 107974404A
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- CN
- China
- Prior art keywords
- filter stick
- autoclave body
- enzyme digestion
- reaction kettle
- digestion reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Thermal Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Accessories For Mixers (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a kind of enzyme digestion reaction kettle, belong to chemical reaction device technical field, including the autoclave body with inlet port and outlet port, blender, peripheral hardware heating jacket are equipped with the autoclave body, filter stick is equipped with the autoclave body bottom discharge port, the filter stick is the hollow tubular of one end closing, and the filter stick side wall is equipped with filter hole, and the filter stick insertion autoclave body is interior and its end is connected with discharge port.Enzyme digestion reaction kettle provided by the invention sets filter stick by the discharge port in autoclave body bottom, it can realize and feed liquid is filtered during discharging, it is returned to without feed liquid to be delivered to the enzyme that will filter out again after filtering tank is filtered in autoclave body, discharging, filter progress are combined into one, technological process is simplified, reduces the waste of enzyme.
Description
Technical field
The invention belongs to chemical reaction device technical field, is to be related to a kind of enzyme digestion reaction kettle more specifically.
Background technology
Reaction kettle is widely used in the industries such as oil, chemical industry, rubber, pesticide, medicine, food, for completing vulcanization, nitre
The pressure vessel of the technical process such as change-hydrogenation, polymerization, condensation.Zymolysis technique is protein engineering, enzyme engineering and bioengineering phase
With reference to product, be widely used in the fields such as medicine, food, feed in recent years, enzyme digestion reaction mainly carries out in reaction kettle.
Enzyme digestion reaction kettle is a kind of comprehensive reaction vessel, its structure function and configuration annex can be designed according to reaction condition,
Temperature of charge, pressure, stirring and reactant concentration etc. are monitored according to technological requirement.
In general, discharged in enzyme digestion reaction kettle after the completion of material reaction from discharge port through pipeline to next process, but
It is that material needs to be filtered into filtering tank before subsequent processing is entered, the enzyme filtered out, which is returned again in reaction kettle, to be repeated
Utilize.A part of enzyme can be lost in this circular flow, causes to waste, causes the increase of production cost.
The content of the invention
It is an object of the invention to provide a kind of enzyme digestion reaction kettle, passes through filter tank to solve material existing in the prior art
Filtering causes the technical problem of part enzyme loss.
To achieve the above object, the technical solution adopted by the present invention is：A kind of enzyme digestion reaction kettle is provided, including with charging
The autoclave body of mouth and discharge port, the autoclave body is interior to be equipped with blender, peripheral hardware heating jacket, is equipped with the autoclave body bottom discharge port
Filter stick, the filter stick are the hollow tubular of one end closing, and the filter stick side wall is equipped with filter hole, the filter stick insertion
In autoclave body and its end is connected with discharge port.
Further, the filter stick is for several and along the spherical kettle bottom circumference of autoclave body lower end, the filtering
The port of export of rod is equipped with the ring flange with discharge port flanged joint.
Further, the filter hole on the filter stick cross section is uniformly arranged, and the filtering hole exits are along outside filter stick
Disc tangential direction is arranged.
Further, the blender includes motor, agitating shaft and paddle, and the paddle is arranged on from top to bottom to be stirred
Mix on axis, the motor is arranged at the top of autoclave body, and the agitating shaft is vertically set in autoclave body and the setting of agitating shaft lower end is spherical
On stent in the middle part of bottom, the stent is fixed on bottom, and self contering structure is equipped between stent and the agitating shaft end.
Further, the self contering structure includes housing, step, makes cartridge and top chock, the housing by oneself
It is arranged on stent, the step and makes cartridge by oneself and be arranged in housing, the step is arranged on housing cavity
Bottom, the shaft end of the agitating shaft through top chock and make cartridge, the shaft end key connection for making cartridge and agitating shaft by oneself by oneself；
The cartridge of making by oneself is coordinated by taper thrustbearing and top chock, step respectively up and down；The top chock and shell
Body connects, and sealing ring is equipped between the upper support seat and agitating shaft.
Further, the housing cavity bottom is equipped with annular convex platform, and spherical support block is equipped with the middle part of the annular convex platform,
The step lower end is equipped with the platform coordinated with annular convex platform periphery and justifies；The upper support seat and housing are by lengthening screw rod
It is fixed on stent.
Further, the cartridge outer surface of making by oneself is equipped with wearing layer.
Further, the stent includes discoid table top and several supporting legs, the supporting leg be arranged on table top surrounding and
Supporting leg lower end is fixed on bottom, and the branch midleg is equipped with filter stick, and the filter stick outlet is connected with discharge port in the middle part of bottom.
Preferably, the paddle is waveform, and the paddle is arranged in pairs on agitating shaft and upper and lower two pairs of stirrings
Leaf interlaced arrangement.
Further, the body material is stainless steel.
The beneficial effect of enzyme digestion reaction kettle provided by the invention is：Compared with prior art, enzymolysis provided by the invention
Reaction kettle sets filter stick by the discharge port in autoclave body bottom, can realize and feed liquid is filtered during discharging, nothing
Feed liquid need to be delivered to the enzyme that will filter out again after filtering tank is filtered to be returned in autoclave body, discharging, filter progress are closed two
For one, technological process is simplified, reduces the waste of enzyme.
Brief description of the drawings
To describe the technical solutions in the embodiments of the present invention more clearly, below will be to embodiment or description of the prior art
Needed in attached drawing be briefly described, it should be apparent that, drawings in the following description be only the present invention some
Embodiment, for those of ordinary skill in the art, without having to pay creative labor, can also be according to these
Attached drawing obtains other attached drawings.
Fig. 1 is the structure diagram of enzyme digestion reaction kettle provided in an embodiment of the present invention；
Fig. 2 is the cross-sectional view of filter stick in Fig. 1；
Fig. 3 is the structure diagram of self contering structure in Fig. 1；
Wherein, each reference numeral in figure：
1- autoclave bodies, 2- blenders, 3- heating jackets, 4- discharge ports, 5- filter sticks, 6- filter hole, 7- stents, 8- housings,
9- steps, 10- make cartridge, 11- upper support seats, 12- taper thrustbearings, 13- sealing rings, 14- annular convex platform, 15- by oneself
Supporting block, 16- table tops, 17- supporting legs, 18- self contering structures；21- motors, 22- agitating shafts, 23- paddles.
Embodiment
In order to which technical problems, technical solutions and advantages to be solved are more clearly understood, tie below
Accompanying drawings and embodiments are closed, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only
To explain the present invention, it is not intended to limit the present invention.
Referring to Fig. 1, existing illustrate enzyme digestion reaction kettle provided by the invention.The enzyme digestion reaction kettle, including with
The autoclave body 1 of inlet port and outlet port, the autoclave body 1 is interior to be equipped with blender 2, peripheral hardware heating jacket 3,1 bottom discharge of autoclave body
Filter stick 5 is equipped with mouth 4, the filter stick 5 is the hollow tubular of one end closing, and 5 side wall of filter stick is equipped with filter hole
6, the filter stick 5 is inserted into autoclave body 1 and its end is connected with discharge port 4.Wherein, 1 material of autoclave body is stainless steel.Add
Hot sheath internal medicine admittance deep fat, or heated using other heat tracing pipes.
Enzyme digestion reaction kettle provided by the invention, compared with prior art, filtering is set by the discharge port in autoclave body bottom
Rod, can realize and feed liquid is filtered during discharging, be incited somebody to action again without feed liquid is delivered to after filtering tank is filtered
The enzyme filtered out is returned in autoclave body, and discharging, filter progress are combined into one, technological process is simplified, reduces the waste of enzyme.
Further, also referring to Fig. 1,2, a kind of specific embodiment party as enzyme digestion reaction kettle provided by the invention
Formula, the filter stick 5 is for several and along the spherical kettle bottom circumference of 1 lower end of autoclave body, the port of export of the filter stick 5
Equipped with the ring flange with 4 flanged joint of discharge port.The installing and dismounting of filter stick is more convenient using flanged joint, is conveniently replaceable, ties up
Repair.
Further optimize above-mentioned technical proposal, referring to Fig. 2, the filter hole 6 on 5 cross section of the filter stick is laid
Put, the filter hole 6 is exported arranges along 5 periphery tangential direction of filter stick.Filter hole is tangentially-arranged along filter stick cylindrical surface,
It can be finished in reaction blowing, purge gas or liquid is formed helical airflow or current in autoclave body, easy to be trapped in
Enzyme near filter stick dispels, and avoids that there are dead angle.
Further, please refer to Fig.1,3, as a kind of embodiment of enzyme digestion reaction kettle provided by the invention, institute
Stating blender 2 includes motor 21, agitating shaft 22 and paddle 23, and the paddle is disposed on agitating shaft 22 from top to bottom
On, the motor 21 is arranged on the top of autoclave body 1, and the agitating shaft 22 is vertically set in autoclave body 1 and 22 lower end of agitating shaft is set
On stent 7 in the middle part of spherical kettle bottom, the stent 7 is fixed on bottom, is equipped between 22 end of stent 7 and agitating shaft self-centering
Structure 18.It can make radial positioning of the agitating shaft under responsive to axial force using self contering structure, prevent agitating shaft in whipping process
Middle generation beat.
Referring to Fig. 3, the self contering structure 18 includes housing 8, step 9, makes cartridge 10 and top chock 11, institute by oneself
State housing 8 to be arranged on stent 7, the step 9 and make cartridge 10 by oneself and be arranged in housing 8, the step 9 is set
Put in 8 intracavity bottom of housing, the shaft end of the agitating shaft 22 through top chock 11 and makes cartridge 10 by oneself, described to make cartridge 10 by oneself
With the shaft end key connection of agitating shaft 22；It is described make by oneself cartridge about 10 respectively by taper thrustbearing 12 and top chock 11,
Step 9 coordinates；The top chock 11 and housing 8 connect, and sealing is equipped between the upper support seat 11 and agitating shaft 22
Circle 13.Taper thrustbearing is fixed by screws on top chock and step, and 10 outer surface of cartridge of making by oneself is equipped with
3Cr13 materials can be selected in wearing layer, wearing layer.
In one particular embodiment of the present invention, annular convex platform 14, institute are equipped with referring to Fig. 3,8 intracavity bottom of housing
State the middle part of annular convex platform 14 and be equipped with spherical support block 15,9 lower end of step is equipped with to be coordinated with 14 periphery of annular convex platform
Platform circle, make step fix it is more firm；The upper support seat 11 and housing 8 are fixed on stent 7 by lengthening screw rod.
Please refer to Fig.1,3, the stent 7 includes discoid table top 16 and several supporting legs 17, and the supporting leg 17 is arranged on
16 surrounding of table top and 17 lower end of supporting leg are fixed on bottom, and the middle part of supporting leg 17 is equipped with filter stick 5, the outlet of filter stick 5 and
Discharge port is connected in the middle part of bottom.Using the filter stick in the middle part of bottom feed liquid can be made fully to discharge.
In a preferred embodiment of the invention, the paddle 23 is waveform, and the paddle 23 is arranged in pairs
On agitating shaft 22 and 23 interlaced arrangement of upper and lower two pairs of paddles.Can fill feed liquid mixing in autoclave body using waveform paddle
Point.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should all be included in the protection scope of the present invention.
Claims (10)
1. enzyme digestion reaction kettle, including the autoclave body with inlet port and outlet port, the autoclave body is interior to be equipped with blender, peripheral hardware heating shield
Set, it is characterised in that：Filter stick is equipped with the autoclave body bottom discharge port, the filter stick is the hollow tubular of one end closing,
The filter stick side wall is equipped with filter hole, and the filter stick insertion autoclave body is interior and its end is connected with discharge port.
2. enzyme digestion reaction kettle as claimed in claim 1, it is characterised in that：The filter stick is for several and along under autoclave body
The spherical kettle bottom circumference at end, the port of export of the filter stick are equipped with the ring flange with discharge port flanged joint.
3. enzyme digestion reaction kettle as claimed in claim 2, it is characterised in that：Filter hole on the filter stick cross section is laid
Put, the filtering hole exits are arranged along filter stick periphery tangential direction.
4. enzyme digestion reaction kettle as claimed in claim 1, it is characterised in that：The blender includes motor, agitating shaft and stirring
Leaf, the paddle are disposed on agitating shaft from top to bottom, and the motor is arranged at the top of autoclave body, and the agitating shaft is vertical
It is arranged in autoclave body and agitating shaft lower end is set on the stent in the middle part of spherical kettle bottom, the stent is fixed on bottom, the stent
Self contering structure is equipped between agitating shaft end.
5. enzyme digestion reaction kettle as claimed in claim 4, it is characterised in that：The self contering structure include housing, step,
Make cartridge and top chock by oneself, the housing is arranged on stent, the step and is made cartridge by oneself and is arranged in housing,
The step is arranged on housing cavity bottom, and the shaft end of the agitating shaft through top chock and makes cartridge by oneself, it is described from
The shaft end key connection of centering disk and agitating shaft；It is described make by oneself cartridge up and down respectively by taper thrustbearing and top chock, under
Bearing block coordinates；The top chock is connected with housing, and sealing ring is equipped between the upper support seat and agitating shaft.
6. enzyme digestion reaction kettle as claimed in claim 5, it is characterised in that：The housing cavity bottom is equipped with annular convex platform, institute
State and spherical support block is equipped with the middle part of annular convex platform, the step lower end is equipped with the platform coordinated with annular convex platform periphery and justifies；
The upper support seat and housing are fixed on stent by lengthening screw rod.
7. enzyme digestion reaction kettle as claimed in claim 5, it is characterised in that：The cartridge outer surface of making by oneself is equipped with wearing layer.
8. enzyme digestion reaction kettle as claimed in claim 5, it is characterised in that：The stent includes discoid table top and several branch
Leg, the supporting leg is arranged on table top surrounding and supporting leg lower end is fixed on bottom, and the branch midleg is equipped with filter stick, the mistake
Filter stick is exported to be connected with discharge port in the middle part of bottom.
9. enzyme digestion reaction kettle as claimed in claim 4, it is characterised in that：The paddle is waveform, the paddle into
To being arranged on agitating shaft and upper and lower two pairs of paddle interlaced arrangements.
10. such as claim 1-9 any one of them enzyme digestion reaction kettles, it is characterised in that：The body material is stainless steel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711044623.9A CN107974404A (en) | 2017-10-31 | 2017-10-31 | Enzyme digestion reaction kettle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711044623.9A CN107974404A (en) | 2017-10-31 | 2017-10-31 | Enzyme digestion reaction kettle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107974404A true CN107974404A (en) | 2018-05-01 |
Family
ID=62012944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711044623.9A Pending CN107974404A (en) | 2017-10-31 | 2017-10-31 | Enzyme digestion reaction kettle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107974404A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109136086A (en) * | 2018-09-26 | 2019-01-04 | 郑州睿科生化科技有限公司 | A kind of device of enzyme process levulic acid esters |
| CN112426998A (en) * | 2020-12-11 | 2021-03-02 | 江苏宏梓新能源科技有限公司 | Reaction kettle for producing sodium borohydride and production process |
| CN116622501A (en) * | 2023-07-24 | 2023-08-22 | 山东中创亿丰肥料集团有限公司 | Enzymolysis device is used in protein polypeptide production |
| CN117986052A (en) * | 2024-04-07 | 2024-05-07 | 阿托菲纳(山东)作物营养有限公司 | Efficient enzymolysis kettle for biological fertilizer enzymolysis reaction |
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| CN101648127A (en) * | 2009-09-09 | 2010-02-17 | 威海化工机械有限公司 | Ceramic bearing type reaction kettle |
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| CN106390894A (en) * | 2016-10-11 | 2017-02-15 | 威海化工机械有限公司 | Extended bottom support type reaction kettle |
| CN206408246U (en) * | 2017-01-12 | 2017-08-15 | 南京红杉生物科技有限公司 | A kind of reactor for producing L Terleus |
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2017
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| CN205741033U (en) * | 2016-05-10 | 2016-11-30 | 四川清山绿水实业发展有限公司 | A kind of uniform enzymatic vessel that feeds |
| CN205821350U (en) * | 2016-07-04 | 2016-12-21 | 西北民族大学 | A kind of enzymolysis agitator tank |
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| 潘家祯，王学生主编: "《化工机械新技术研究进展》", 31 July 2008 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109136086A (en) * | 2018-09-26 | 2019-01-04 | 郑州睿科生化科技有限公司 | A kind of device of enzyme process levulic acid esters |
| CN112426998A (en) * | 2020-12-11 | 2021-03-02 | 江苏宏梓新能源科技有限公司 | Reaction kettle for producing sodium borohydride and production process |
| CN116622501A (en) * | 2023-07-24 | 2023-08-22 | 山东中创亿丰肥料集团有限公司 | Enzymolysis device is used in protein polypeptide production |
| CN116622501B (en) * | 2023-07-24 | 2023-09-22 | 山东中创亿丰肥料集团有限公司 | Enzymolysis device is used in protein polypeptide production |
| CN117986052A (en) * | 2024-04-07 | 2024-05-07 | 阿托菲纳(山东)作物营养有限公司 | Efficient enzymolysis kettle for biological fertilizer enzymolysis reaction |
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Effective date of registration: 20210806 Address after: 441141 Xiangcheng Economic Development Zone, Xiangcheng District, Xiangyang City, Hubei Province Applicant after: HUBEI LINGSHENG PHARMACEUTICAL Co.,Ltd. Address before: 441141 No. 1, No. 10 road, Xiangcheng Economic Development Zone, Xiangyang City, Hubei Province Applicant before: HUBEI LINGSHENG PHARMACEUTICAL Co.,Ltd. Applicant before: WUHAN INSTITUTE OF TECHNOLOGY |