CN116622501B - Enzymolysis device is used in protein polypeptide production - Google Patents
Enzymolysis device is used in protein polypeptide production Download PDFInfo
- Publication number
- CN116622501B CN116622501B CN202310904728.6A CN202310904728A CN116622501B CN 116622501 B CN116622501 B CN 116622501B CN 202310904728 A CN202310904728 A CN 202310904728A CN 116622501 B CN116622501 B CN 116622501B
- Authority
- CN
- China
- Prior art keywords
- fixedly connected
- rotating shaft
- reaction kettle
- protein
- frame
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 74
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 74
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 14
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 14
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 14
- 238000004519 manufacturing process Methods 0.000 title description 5
- 238000003756 stirring Methods 0.000 claims abstract description 48
- 238000006243 chemical reaction Methods 0.000 claims abstract description 44
- 230000007246 mechanism Effects 0.000 claims description 40
- 238000002156 mixing Methods 0.000 claims description 30
- 230000005540 biological transmission Effects 0.000 claims description 21
- 230000007306 turnover Effects 0.000 claims description 16
- 238000001914 filtration Methods 0.000 claims description 15
- 239000002994 raw material Substances 0.000 abstract description 42
- 102000004190 Enzymes Human genes 0.000 abstract description 39
- 108090000790 Enzymes Proteins 0.000 abstract description 39
- 239000007788 liquid Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000017854 proteolysis Effects 0.000 description 2
- 238000007790 scraping Methods 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/08—Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of protein enzymolysis, in particular to an enzymolysis device for producing protein polypeptide. The invention can more uniformly and fully mix the protein raw material and the enzyme, so that the protein is fully and rapidly hydrolyzed, and the enzymolysis efficiency of the protein is more effectively improved. The invention comprises a mounting rack, a fixing rack, a reaction kettle and the like; two fixing frames are arranged on the mounting frame, and a reaction kettle is arranged between the two fixing frames. According to the invention, the protein raw material and the enzyme in the reaction kettle are primarily stirred by rotating the stirring blades, the protein raw material and the enzyme are mixed, so that the enzyme is used for carrying out enzymolysis on the protein, meanwhile, the protein raw material and the enzyme are further stirred by the left and right reciprocating movement of the stirring blades, the protein raw material and the enzyme at the periphery of the inner wall of the reaction kettle and at the middle position of the reaction kettle are mixed, the protein raw material and the enzyme are uniformly mixed, and the enzymolysis efficiency of the protein is further improved.
Description
Technical Field
The invention relates to the technical field of protein enzymolysis, in particular to an enzymolysis device for producing protein polypeptide.
Background
Proteolysis refers to the process of degrading protein into amino acid under the action of various proteases, polypeptide is intermediate product of proteolysis, and is usually formed by dehydration condensation of three or more amino acid molecules, and enzyme is a catalyst, and the enzyme participates in the process to effectively reduce activation energy and increase reaction rate, so that the decomposition reaction participated by the enzyme is called enzymolysis reaction, and the current production mode of polypeptide in China is mainly enzymolysis.
The enzymolysis of protein is generally carried out by using a special enzymolysis device, and an enzymolysis reaction kettle is disclosed at present, the publication number is CN107974404A, the enzymolysis reaction kettle comprises a kettle body with a feed inlet and a discharge outlet, a stirrer and a heating jacket are arranged in the kettle body, a filter rod is arranged in the discharge outlet at the bottom of the kettle body, the filter rod is hollow tubular with one end being sealed, a filter hole is arranged on the side wall of the filter rod, and the filter rod is inserted into the kettle body and the end part of the filter rod is connected with the discharge outlet. An enzymolysis reaction kettle is provided with a filter rod at a discharge hole at the bottom of the kettle body, so that the feed liquid can be filtered in the discharge process, the feed liquid is not required to be conveyed to a filter tank for filtering, and filtered enzyme is returned to the kettle body, so that the discharge and the filtering processes are combined into one, the process flow is simplified, and the waste of the enzyme is reduced.
The protein raw material and the enzyme are stirred in a unidirectional rotation manner by the reaction kettle, and cannot be sufficiently stirred, so that the protein raw material and the enzyme cannot be sufficiently and uniformly mixed, the enzymolysis efficiency of the protein can be reduced, and the enzymolysis efficiency of the protein cannot be effectively improved.
Disclosure of Invention
The invention aims to overcome the defects of the technology, and provides an enzymolysis device for producing protein polypeptide, which can more uniformly and fully mix protein raw materials and enzymes, so that the protein is subjected to enzymolysis more fully and quickly, and the enzymolysis efficiency of the protein is improved more effectively.
The technical scheme is as follows: the utility model provides a protein polypeptide production is with enzymolysis device, includes mounting bracket, mount, reation kettle, stirring mechanism and filtering mechanism, fixedly connected with two mounts on the mounting bracket, two fixedly connected with reation kettle between the mount, stirring mechanism establishes on the mounting bracket, filtering mechanism establishes on stirring mechanism.
In addition, particularly preferred, stirring mechanism is including motor, pivot, slide bar, slider, stirring blade, curved ring, connecting rod and a spring, motor fixed connection is at the top of mounting bracket, the output shaft and the mounting bracket rotation type of motor are connected, the pivot rotation type is connected at the top of mounting bracket, the output shaft fixed connection of pivot and motor, slide bar fixed connection is in the pivot, the slide bar is located reation kettle, the slider slidingtype is connected on the slide bar, stirring blade rotation type is connected in the slider bottom, curved ring fixed connection is at reation kettle's top, curved groove has been seted up on the curved ring, connecting rod fixed connection is at the slider top, connecting rod and curved ring sliding connection, be connected with a spring between slider and the pivot.
In addition, particularly preferred, filtering mechanism is including piston, fixture block, cartridge filter and No. two springs, the piston cup joints in the pivot lower part, the piston is connected with reation kettle slidingtype, the piston bottom rotation type is connected with two fixture blocks, two spouts have been seted up to the reation kettle bottom, the fixture block passes the spout, cartridge filter fixed connection is in the reation kettle bottom, the piston is located the cartridge filter directly over, be connected with No. two springs between piston and the pivot.
In addition, particularly preferred is, still including tilting mechanism, tilting mechanism establishes in the pivot, tilting mechanism is including dish frame, turnover board, awl dish, drive rack, drive gear and No. three springs, the dish frame is rotationally connected in the pivot, be connected with a plurality of turnover board on the dish frame, the awl dish cup joints in the pivot, the awl dish is located dish frame top, fixedly connected with a plurality of drive rack on the awl dish, every all fixedly connected with drive gear on the turnover board, drive rack and drive gear meshing, be connected with No. three springs between dish frame and the awl dish.
In addition, particularly preferred is, still include and mix the even mechanism, mix the even mechanism and establish in the pivot, mix even mechanism including the connecting plate, mix even frame, drive rack and drive gear, connecting plate fixed connection is in the pivot, mix even frame swivelling joint on the connecting plate, drive rack fixed connection is on the connecting rod, drive gear fixed connection is in mixing even frame top, drive rack and drive gear engagement.
In addition, it is particularly preferred that a transverse rack fixedly connected to the connection plate and a transverse gear fixedly connected to the stirring blade are also included, the transverse gear being in engagement with the transverse rack.
In addition, it is particularly preferred that a scraper is also included, which is fixedly connected to the shaft and which is in contact with the filter cartridge.
Compared with the prior art, the invention has the beneficial effects that: 1. according to the invention, the stirring blades and the connecting rods are driven to rotate by the motor, so that the stirring blades can primarily stir protein raw materials and enzymes in the reaction kettle, the protein raw materials and the enzymes are mixed, the enzymes carry out enzymolysis on the protein, the connecting rods can move left and right in a reciprocating manner along the curved ring, the stirring blades move left and right in a reciprocating manner, the protein raw materials and the enzymes are further stirred by the left and right reciprocating manner of the stirring blades, the protein raw materials and the enzymes around the inner wall of the reaction kettle and in the middle of the reaction kettle are mixed, the protein raw materials and the enzymes can be uniformly mixed, and the enzymolysis efficiency of the protein is further improved.
2. According to the invention, the stirring blades move left and right to push the conical disc to reciprocate up and down, so that the plurality of transmission racks reciprocate up and down, and then the plurality of overturning plates are driven to reciprocate up and down, the plurality of overturning plates reciprocate up and down to stir protein raw materials precipitated at the lower part of the reaction kettle, and then the protein raw materials at the bottom part in the reaction kettle are overturned, and the stirring blades are used for fully mixing the protein raw materials with enzymes, so that the proteins can be fully hydrolyzed, and the enzymolysis efficiency of the proteins is improved.
3. According to the invention, the mixing frame is driven to rotate through the rotation of the rotating shaft, the protein raw material turned up by the turning plate is further stirred, and meanwhile, the connecting rod moves in a left-right reciprocating manner to drive the driving rack to move in a left-right reciprocating manner, so that the driving gear rotates in a reciprocating manner, and further the mixing frame is driven to rotate in a reciprocating manner, so that the mixing frame can more fully stir the protein raw material and the enzyme, the mixing speed of the protein raw material and the enzyme is improved, and the mixing frame is positioned at the other side of the stirring blade, so that the mixing frame can be matched with the stirring blade, the protein raw material and the enzyme in the reaction kettle are more comprehensively and uniformly mixed, and the enzymolysis efficiency of the protein is more effectively improved.
Drawings
Fig. 1 is a schematic perspective view of the present invention.
Fig. 2 is a schematic view of a partially cut-away perspective structure of the present invention.
Fig. 3 is a schematic view of a partial perspective view of the agitation mechanism and the filtering mechanism of the present invention.
Fig. 4 is a schematic view of a partial perspective structure of the present invention.
Fig. 5 is a schematic view of a partial perspective structure of the filter mechanism and the turnover mechanism of the present invention.
Marked in the figure as: 1-mounting frame, 2-fixing frame, 3-reaction kettle, 41-motor, 42-rotating shaft, 43-sliding rod, 44-sliding block, 45-stirring blade, 46-curved ring, 47-connecting rod, 48-spring, 51-piston, 52-clamping block, 53-sliding groove, 54-filter cartridge, 55-spring II, 61-tray frame, 62-turnover plate, 63-conical tray, 64-transmission rack, 65-transmission gear, 66-spring III, 71-connecting plate, 72-mixing frame, 73-driving rack, 74-driving gear, 8-transverse rack, 9-transverse gear and 10-scraper.
Detailed Description
In order to make the object technical scheme and advantages of the present invention more apparent, the present invention will be further described in detail with reference to the accompanying drawings and examples. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Example 1: the utility model provides a protein polypeptide production is with enzymolysis device, as shown in fig. 1-5, including mounting bracket 1, mount 2, reation kettle 3, stirring mechanism and filtering mechanism, there are two mounts 2 on the mounting bracket 1 through bolted connection, two there is reation kettle 3 through bolted connection between the mount 2, stirring mechanism establishes on mounting bracket 1, filtering mechanism establishes on stirring mechanism.
The stirring mechanism comprises a motor 41, a rotating shaft 42, a sliding rod 43, a sliding block 44, stirring blades 45, a curved ring 46, a connecting rod 47 and a number spring 48, wherein the motor 41 is connected to the top of the mounting frame 1 through bolts, an output shaft of the motor 41 is rotationally connected to the mounting frame 1, the rotating shaft 42 is rotationally connected to the top of the mounting frame 1, the rotating shaft 42 is vertically arranged, the rotating shaft 42 is fixedly connected with the output shaft of the motor 41, the sliding rod 43 is fixedly connected to the rotating shaft 42, the sliding rod 43 is horizontally arranged, the sliding rod 43 is positioned in the reaction kettle 3, the sliding block 44 is slidably connected to the sliding rod 43, the stirring blades 45 are rotationally connected to the bottom of the sliding block 44, the curved ring 46 is fixedly connected to the top of the reaction kettle 3, a curved groove is formed in the curved ring 46, the connecting rod 47 is fixedly connected to the top of the sliding block 44, the connecting rod 47 is slidably connected to the curved ring 46, a number spring 48 is connected between the sliding block 44 and the rotating shaft 42, and the number spring 48 is sleeved on the sliding rod 43.
The filtering mechanism comprises a piston 51, a clamping block 52, a filtering cylinder 54 and a second spring 55, wherein the piston 51 is sleeved on the lower portion of the rotating shaft 42, the piston 51 is connected with the reaction kettle 3 in a sliding mode, two clamping blocks 52 are connected to the bottom of the piston 51 in a rotating mode, two sliding grooves 53 are formed in the bottom of the reaction kettle 3, the clamping blocks 52 penetrate through the sliding grooves 53, the filtering cylinder 54 is connected to the bottom of the reaction kettle 3 through bolts, the piston 51 is located right above the filtering cylinder 54, the second spring 55 is connected between the piston 51 and the rotating shaft 42, and the second spring 55 is sleeved on the rotating shaft 42.
Initially, because the reaction kettle 3 is clamped with the two clamping blocks 52, the second spring 55 is in a stretched state, in actual operation, a worker firstly adds protein raw materials and enzymes into the reaction kettle 3, then uses the reaction kettle 3 to heat the protein raw materials and enzymes, simultaneously starts the motor 41, the output shaft of the motor 41 rotates to drive the rotating shaft 42 and the sliding rod 43 to rotate together, the sliding rod 43 rotates to drive the sliding block 44, the stirring blade 45 and the connecting rod 47 to rotate together, the stirring blade 45 rotates to primarily stir the protein raw materials and enzymes in the reaction kettle 3, the protein raw materials and enzymes are mixed, the enzymes carry out enzymolysis on the proteins, the connecting rod 47 rotates and simultaneously reciprocates left and right along Qu Xingcao of the curved ring 46, the connecting rod 47 reciprocates left and right to drive the sliding block 44 and the stirring blade 45 to reciprocate left and right together, the first spring 48 is continuously compressed, the left and right reciprocating movement of the stirring blades 45 can further stir the protein raw materials and enzymes, the protein raw materials and enzymes around the inner wall of the reaction kettle 3 and in the middle of the reaction kettle 3 are mixed, the protein raw materials and enzymes can be uniformly mixed, the enzymolysis efficiency of the protein is further improved, after the enzymolysis of the protein and the polypeptide are finished, a worker turns off the motor 41 and rotates the two clamping blocks 52 by 90 degrees, the reaction kettle 3 does not clamp the two clamping blocks 52 any more, the piston 51 is driven to move upwards by the reset of the second spring 55, the piston 51 moves upwards and drives the two clamping blocks 52 to pass through the two sliding grooves 53, the filter cartridge 54 is not blocked any more by the upward movement of the piston 51, the feed liquid in the reaction kettle 3 is discharged from the filter cartridge 54, the filter cartridge 54 can filter the feed liquid, the enzyme and the unreacted protein raw materials are left in the reaction kettle 3, so that the protein raw materials are subjected to enzymolysis again, after the material liquid is discharged, the worker pulls the two clamping blocks 52 to move downwards, the piston 51 is driven to move downwards by the two clamping blocks 52 to move downwards, the second spring 55 is stretched, the filter cartridge 54 is blocked by the piston 51 moving downwards, and then the worker rotates the two clamping blocks 52 by 90 degrees reversely, so that the two clamping blocks 52 are clamped by the reaction kettle 3.
Example 2: on the basis of embodiment 1, as shown in fig. 2-5, the turnover mechanism is arranged on the rotating shaft 42, the turnover mechanism comprises a disc frame 61, turnover plates 62, conical discs 63, transmission racks 64, transmission gears 65 and a third spring 66, the disc frame 61 is rotatably connected to the rotating shaft 42, a plurality of turnover plates 62 are rotatably connected to the disc frame 61, the conical discs 63 are sleeved on the rotating shaft 42, the conical discs 63 are positioned above the disc frame 61, a plurality of transmission racks 64 are welded on the conical discs 63, each turnover plate 62 is fixedly connected with a transmission gear 65, the transmission racks 64 are meshed with the transmission gears 65, a third spring 66 is connected between the disc frame 61 and the conical discs 63, and the third spring 66 is sleeved on the rotating shaft 42.
When the protein raw material is subjected to enzymolysis, a part of the protein raw material can be precipitated at the lower part of the reaction kettle 3, so that the part of the protein raw material cannot be subjected to enzymolysis, the stirring blade 45 contacts the conical disk 63 when moving to the side close to the rotating shaft 42 and extrudes the conical disk 63 to move downwards, the third spring 66 is compressed, the conical disk 63 moves downwards to drive the plurality of transmission racks 64 to move downwards together, the transmission racks 64 move downwards to drive the transmission gear 65 to rotate, the transmission gear 65 rotates to drive the turnover plate 62 to swing downwards, the stirring blade 45 moves to the side far away from the rotating shaft 42 and is separated from the conical disk 63 to be in contact with the third spring 66 to reset together with the plurality of transmission racks 64, the transmission racks 64 reset upwards to drive the transmission gear 65 to rotate reversely, the transmission gear 65 rotates reversely to drive the turnover plate 62 to swing upwards, and repeatedly so as to drive the plurality of turnover plates 62 to swing up and down, and further the protein raw material at the bottom of the reaction kettle 3 is turned over, and the protein raw material is fully mixed with the enzyme through the stirring blade 45, so that the enzymolysis efficiency of the protein raw material can be fully improved.
Example 3: on the basis of embodiment 2, as shown in fig. 2-4, the device further comprises a mixing mechanism, the mixing mechanism is arranged on the rotating shaft 42, the mixing mechanism comprises a connecting plate 71, a mixing frame 72, a driving rack 73 and a driving gear 74, the connecting plate 71 is welded on the rotating shaft 42, the mixing frame 72 is rotatably connected on the connecting plate 71, the driving rack 73 is fixedly connected on the connecting rod 47, the driving gear 74 is fixedly connected at the top end of the mixing frame 72, and the driving rack 73 is meshed with the driving gear 74.
The rotation of the rotating shaft 42 drives the connecting plate 71, the mixing rack 72 and the driving gear 74 to rotate together, the rotation of the mixing rack 72 further stirs protein raw materials turned up by the turning plate 62, the rotation of the connecting rod 47 drives the driving rack 73 to rotate, the movement of the connecting rod 47 to one side close to the rotating shaft 42 drives the driving rack 73 to move, the movement of the driving rack 73 drives the driving gear 74 and the mixing rack 72 to rotate together, the movement of the connecting rod 47 to one side far away from the rotating shaft 42 drives the driving rack 73 to reset, the reset of the driving rack 73 drives the driving gear 74 and the mixing rack 72 to rotate reversely together, and the like is repeated, so that the mixing rack 72 rotates reciprocally while rotating, the mixing rack 72 more fully stirs protein raw materials and enzymes, the mixing speed of the protein raw materials and enzymes is improved, and the mixing rack 72 is positioned on the other side of the stirring blade 45, so that the mixing rack 72 can be matched with the stirring blade 45 to more comprehensively mix the protein raw materials and enzymes in the reaction kettle 3, and the enzymolysis efficiency of the protein is improved more effectively.
Example 4: on the basis of the embodiment 3, as shown in fig. 2-4, the device further comprises a transverse rack 8 and a transverse gear 9, wherein the transverse rack 8 is fixedly connected to the connecting plate 71, the transverse gear 9 is fixedly connected to the stirring blade 45, and the transverse gear 9 is meshed with the transverse rack 8.
The stirring blade 45 can drive the transverse gear 9 to reciprocate left and right while reciprocating left and right, the transverse gear 9 can reciprocate along the transverse rack 8 while reciprocating left and right, the transverse gear 9 can drive the stirring blade 45 to reciprocate, and the stirring blade 45 can further turn protein raw materials and enzymes in the reaction kettle 3 to mix more uniformly.
Example 5: on the basis of the embodiment 4, as shown in fig. 2, the filter cartridge further comprises a scraper 10, wherein the scraper 10 is fixedly connected to the rotating shaft 42, and the scraper 10 is in contact with the filter cartridge 54.
The rotation of the rotating shaft 42 drives the scraping plate 10 to rotate, and the rotation of the scraping plate 10 scrapes the filter cartridge 54, so that protein raw materials are prevented from remaining on the filter cartridge 54, and the filter cartridge 54 is blocked.
The embodiments of the present invention have been described above with reference to the accompanying drawings, but the present invention is not limited to the above-described embodiments, which are merely illustrative and not restrictive, and many forms may be made by those having ordinary skill in the art without departing from the spirit of the present invention and the scope of the claims, which are all within the protection of the present invention.
Claims (4)
1. The enzymolysis device for producing the protein polypeptide is characterized by comprising a mounting frame (1), a fixing frame (2), a reaction kettle (3), an agitating mechanism and a filtering mechanism, wherein the two fixing frames (2) are fixedly connected to the mounting frame (1), the reaction kettle (3) is fixedly connected between the two fixing frames (2), the agitating mechanism is arranged on the mounting frame (1), and the filtering mechanism is arranged on the agitating mechanism;
the stirring mechanism comprises a motor (41), a rotating shaft (42), a sliding rod (43), a sliding block (44), stirring blades (45), a curved ring (46), a connecting rod (47) and a spring (48), wherein the motor (41) is fixedly connected to the top of the mounting frame (1), an output shaft of the motor (41) is rotationally connected with the mounting frame (1), the rotating shaft (42) is rotationally connected to the top of the mounting frame (1), the rotating shaft (42) is fixedly connected with the output shaft of the motor (41), the sliding rod (43) is fixedly connected to the rotating shaft (42), the sliding rod (43) is located in the reaction kettle (3), the sliding block (44) is slidably connected to the sliding rod (43), the stirring blades (45) are rotationally connected to the bottom of the sliding block (44), the curved ring (46) is fixedly connected to the top of the reaction kettle (3), a curved groove is formed in the curved ring (46), the connecting rod (47) is fixedly connected to the top of the sliding block (44), and the sliding rod (47) is slidably connected with the sliding ring (44), and the spring (48) is connected between the sliding rod (46) and the sliding rod (42). The filtering mechanism comprises a piston (51), clamping blocks (52), a filter cartridge (54) and a second spring (55), wherein the piston (51) is sleeved on the lower portion of a rotating shaft (42), the piston (51) is connected with a reaction kettle (3) in a sliding mode, two clamping blocks (52) are rotatably connected to the bottom of the piston (51), two sliding grooves (53) are formed in the bottom of the reaction kettle (3), the clamping blocks (52) penetrate through the sliding grooves (53), the filter cartridge (54) is fixedly connected to the bottom of the reaction kettle (3), the piston (51) is located right above the filter cartridge (54), and the second spring (55) is connected between the piston (51) and the rotating shaft (42);
the turnover mechanism is arranged on the rotating shaft (42), and comprises a disc frame (61), turnover plates (62), conical discs (63), transmission racks (64), transmission gears (65) and three springs (66), wherein the disc frame (61) is rotatably connected to the rotating shaft (42), a plurality of turnover plates (62) are rotatably connected to the disc frame (61), the conical discs (63) are sleeved on the rotating shaft (42), the conical discs (63) are located above the disc frame (61), a plurality of transmission racks (64) are fixedly connected to the conical discs (63), each turnover plate (62) is fixedly connected with a transmission gear (65), the transmission racks (64) are meshed with the transmission gears (65), three springs (66) are connected between the disc frame (61) and the conical discs (63), and the three springs (66) are sleeved on the rotating shaft (42).
2. The enzymolysis device for producing protein polypeptide according to claim 1, further comprising a mixing mechanism, wherein the mixing mechanism is arranged on the rotating shaft (42), the mixing mechanism comprises a connecting plate (71), a mixing frame (72), a driving rack (73) and a driving gear (74), the connecting plate (71) is fixedly connected to the rotating shaft (42), the mixing frame (72) is rotatably connected to the connecting plate (71), the driving rack (73) is fixedly connected to the connecting rod (47), the driving gear (74) is fixedly connected to the top end of the mixing frame (72), and the driving rack (73) is meshed with the driving gear (74).
3. An enzymolysis device for producing a protein polypeptide according to claim 2, further comprising a transverse rack (8) and a transverse gear (9), wherein the transverse rack (8) is fixedly connected to the connecting plate (71), the transverse gear (9) is fixedly connected to the stirring blade (45), and the transverse gear (9) is meshed with the transverse rack (8).
4. An enzymolysis device for producing a protein polypeptide according to claim 3, further comprising a scraper (10), wherein the scraper (10) is fixedly connected to the rotary shaft (42), and the scraper (10) is in contact with the filter cartridge (54).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310904728.6A CN116622501B (en) | 2023-07-24 | 2023-07-24 | Enzymolysis device is used in protein polypeptide production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310904728.6A CN116622501B (en) | 2023-07-24 | 2023-07-24 | Enzymolysis device is used in protein polypeptide production |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116622501A CN116622501A (en) | 2023-08-22 |
| CN116622501B true CN116622501B (en) | 2023-09-22 |
Family
ID=87636920
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310904728.6A Active CN116622501B (en) | 2023-07-24 | 2023-07-24 | Enzymolysis device is used in protein polypeptide production |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116622501B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117660152A (en) * | 2023-12-12 | 2024-03-08 | 海南华研胶原科技股份有限公司 | Purification device and purification method for specific protease |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5886929B1 (en) * | 2014-10-23 | 2016-03-16 | 佐竹化学機械工業株式会社 | Stirrer |
| CN107974404A (en) * | 2017-10-31 | 2018-05-01 | 湖北凌晟药业有限公司 | Enzyme digestion reaction kettle |
| CN114797733A (en) * | 2022-06-29 | 2022-07-29 | 东营市宝泽能源科技有限公司 | Corrosion inhibitor preparation device with quantitative charging function |
| CN115430318A (en) * | 2022-07-20 | 2022-12-06 | 电子科技大学 | Preparation device of dicationic catalyst for nitrate radical reduction |
-
2023
- 2023-07-24 CN CN202310904728.6A patent/CN116622501B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5886929B1 (en) * | 2014-10-23 | 2016-03-16 | 佐竹化学機械工業株式会社 | Stirrer |
| CN107974404A (en) * | 2017-10-31 | 2018-05-01 | 湖北凌晟药业有限公司 | Enzyme digestion reaction kettle |
| CN114797733A (en) * | 2022-06-29 | 2022-07-29 | 东营市宝泽能源科技有限公司 | Corrosion inhibitor preparation device with quantitative charging function |
| CN115430318A (en) * | 2022-07-20 | 2022-12-06 | 电子科技大学 | Preparation device of dicationic catalyst for nitrate radical reduction |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116622501A (en) | 2023-08-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN116622501B (en) | Enzymolysis device is used in protein polypeptide production | |
| CN116764497B (en) | Boehmite's preparation facilities | |
| CN116037040A (en) | Polyester granulator | |
| CN113198385A (en) | Automatic feeding device for wet granulator for pharmacy and application process | |
| CN217829754U (en) | Emulsion explosive raw material mixing device | |
| CN116617991A (en) | Paddle type stirring reaction kettle | |
| CN216499358U (en) | Reation kettle is used in catalyst production | |
| CN217614704U (en) | A agitating unit for chemical product makes | |
| CN215540456U (en) | Industrial detergent homogenizing device | |
| CN215139382U (en) | A compounding equipment for production of biological organic milk fertilizer | |
| CN108479550B (en) | V-shaped efficient mixer | |
| CN219631299U (en) | Stirring device for reaction kettle | |
| CN208465827U (en) | A kind of replaceable pot wet granulator | |
| CN112755877A (en) | Stirring method of rotary deflection high-speed axial flow stirring robot group | |
| CN111850057A (en) | Preparation method of solid lactic acid | |
| CN215877961U (en) | Reation kettle for chemical industry that mixing effect is good | |
| CN220425155U (en) | Compounding device for composite biological enzyme preparation | |
| CN221062742U (en) | Reaction kettle for improving product reaction rate | |
| CN214725494U (en) | Raw material stirring device for plastic tableware processing | |
| CN219333853U (en) | Automatic paste adding device for shrimp feed processing | |
| CN219355994U (en) | Reation kettle mixing apparatus | |
| CN220658261U (en) | Mixed feed raw material impurity filtering device | |
| CN218795438U (en) | Powdered food mixer | |
| CN114618370B (en) | Spiral stirrer for agricultural product processing | |
| CN220861450U (en) | Polyurethane reation kettle rabbling mechanism |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: An enzymatic hydrolysis device for protein peptide production Granted publication date: 20230922 Pledgee: Postal Savings Bank of China Limited Changle County sub branch Pledgor: SHANDONG Z.C YIFENG FERTILIZER GROUP CO.,LTD. Registration number: Y2024980001090 |