CN107966558A - A kind of target target molecule quick capturing method for in-vitro diagnosis - Google Patents

A kind of target target molecule quick capturing method for in-vitro diagnosis Download PDF

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CN107966558A
CN107966558A CN201711158456.0A CN201711158456A CN107966558A CN 107966558 A CN107966558 A CN 107966558A CN 201711158456 A CN201711158456 A CN 201711158456A CN 107966558 A CN107966558 A CN 107966558A
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target
fixation support
target molecule
liquid sample
vitro diagnosis
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魏双施
李勇
段生宝
蒙青林
王红梅
丁少华
陈晔洲
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Suzhou Institute of Biomedical Engineering and Technology of CAS
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Suzhou Institute of Biomedical Engineering and Technology of CAS
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures

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  • Life Sciences & Earth Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
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  • Food Science & Technology (AREA)
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  • Analytical Chemistry (AREA)
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Abstract

This case is related to a kind of target target molecule quick capturing method for in-vitro diagnosis, which is present in liquid sample, which is added dropwise on fixation support, the fixation support is equipped with the capture agent of specificity capture target target molecule；Wherein, the side of the fixation support is detachably connected an airtight environment, which has air pressure adjustable function；By adjusting the air pressure of the airtight environment, liquid sample is moved back and forth in the fixation support but do not depart from the fixation support.By the present invention in that liquid sample iterates through fixation support, add the contact frequency of the target target molecule with capture agent, the capture rate of target target molecule is effectively increased, while this method can be used for the matter detergent of non-specific binding again, to reduce background interference.

Description

A kind of target target molecule quick capturing method for in-vitro diagnosis
Technical field
The invention belongs to external quick diagnosis field, and in particular to a kind of target target molecule for in-vitro diagnosis is quickly caught Obtain method.
Background technology
Analysis and diagnostic techniques are always a part fast-developing in in-vitro diagnosis (IVD).Biochemical, immune and molecule, Clinical labororatory's diagnosis and care diagnostic (POCT) can be divided into.Detection object also includes hormone small molecule, antigen and antibody, nucleic acid Deng.Most detection technique be all based on occurring between two kinds or more of components directly or indirectly specific recognition with Association reaction, so as to form detectable the multiple element compound.Most commonly used capture agent is exactly antibody, it can be with various samples Target target molecule (certain species specific antigen, as hepatitis B surface resists in this (such as whole blood, blood plasma, serum, urine, saliva) It is former) combine, by indicating that the display of system produces positive reaction signal.Continuous progress and method battle array with technology, catches Matter and the scope of detection material are all expanding, for example the specificity junction mixture that phage display peptide library screens can be convenient Prepare and its affinity also greatly improves.Development for many years proves that immuno analytical method has great in clinical detection Value.
However, it is accurate in order to obtain as a result, immunological testing usually needs a series of complicated and time-consuming step, also need Accurate it can just be operated with professional technological know-how.In recent years, although there is the detection method of various POCT, as colloidal gold is lateral The appearance of analytical technology is flowed, shortens time and the step of detection, but causes its sensitivity relatively low due to its own, Also the development and application of colloidal gold lateral flow assay technology be greatly limit.
In order to realize this purpose, the in-vitro diagnosis that is used for for having generated substantial amounts of variously-shaped, construction and design is examined Survey device.Substantially, be all by specific catches it is upright connect or be indirectly fixed on detection carrier on, then detect sample In whether there is target substance.If there are target substance in sample, it will be combined in capture agent, then can be with a variety of Instruction system shows target substance present in sample.
Classical is broadly divided into enzyme linked immunosorbent assay and Gold standard, the reaction mechanism that enzyme linked immunosorbent assay uses for in-vitro diagnosis detection method It is exactly common orifice plate, as shown in figure 4, the b modifications of detection carrier can produce specific recognition in orifice plate a bottoms, prepare liquid c The single surface of detection carrier b is only limitted to the interface of association reaction, therefore is that incubation period is longer the shortcomings that this reaction mechanism, Cause whole detection time length (usually when 3 is small or so), sensitivity can reach 50pg/mL or so.The reaction that Gold standard uses Mechanism is based primarily upon test strips, and (the being usually NC films) modification of detection carrier in test strips, be inserted into be measured by when detection by test strips It is added in the sample pad of test strips in liquid or by sample drop to be checked, by the siphonage of test strips other end blotting paper, makes Sample to be checked is unidirectional, once-through detection carrier, therefore is catches the shortcomings that this reaction mechanism and goal response thing connects Touch that the time is short, and Percentage bound is low, ultimately result in that its detection sensitivity is low (can reach 10ng/mL or so), and advantage is that detection speed is fast, Usually result was can obtain at 15-30 minutes.
The content of the invention
For shortcoming of the prior art, the present invention is intended to provide a kind of target target molecule for in-vitro diagnosis is fast Fast catching method, to which reaction efficiency and detection sensitivity can be taken into account at the same time.
To achieve the above object, technical scheme is as follows：
A kind of target target molecule quick capturing method for in-vitro diagnosis, the target target molecule are present in liquid sample In, which is added dropwise on fixation support, the fixation support is equipped with specificity capture target target molecule Capture agent；
Wherein, the side of the fixation support is detachably connected an airtight environment, and the airtight environment is adjustable with air pressure Function；
By adjusting the air pressure of the airtight environment, liquid sample is moved back and forth in the fixation support but do not depart from this Fixation support.
Preferably, the target target molecule quick capturing method for in-vitro diagnosis, wherein, the immobilization carries Body has the space structure of layer stereo micropore, by controlling the air pressure of the airtight environment, iterates through liquid sample described Layer stereo micropore in fixation support.
Preferably, the target target molecule quick capturing method for in-vitro diagnosis, wherein, the immobilization carries Body is arranged to：When liquid sample is added dropwise in fixation support, under normal atmospheric conditions, only relying on gravity does not make liquid Hanging drop occurs for sample.
Preferably, the target target molecule quick capturing method for in-vitro diagnosis, wherein, the airtight environment It is arranged to the reciprocating motion for not interfering liquid sample.
Preferably, the target target molecule quick capturing method for in-vitro diagnosis, wherein, the immobilization carries Body is arranged to the combination for not interfering target target molecule and capture agent.
Preferably, the target target molecule quick capturing method for in-vitro diagnosis, wherein, the immobilization carries The form of body is arranged to membrane type, pillar or cotton-shaped.
The beneficial effects of the invention are as follows：By the present invention in that liquid sample iterates through fixation support, target is added The contact frequency of the target molecule with capture agent, effectively increases the capture rate of target target molecule, while this method can be used for again The matter detergent of non-specific binding, to reduce background interference.
Brief description of the drawings
Fig. 1 is the structure diagram that a specific embodiment used by target target molecule fast Acquisition is realized in this case.
Fig. 2 is view of the liquid sample in decompression.
Fig. 3 is view of the liquid sample in supercharging.
Fig. 4 is the principle schematic for capturing target target molecule based on enzyme linked immunosorbent assay in the prior art.
Embodiment
The present invention is described in further detail below in conjunction with the accompanying drawings, to make those skilled in the art with reference to specification text Word can be implemented according to this.
The target target molecule quick capturing method for in-vitro diagnosis of one embodiment of this case, the target point is listed below Son is present in liquid sample, which is added dropwise on fixation support, fixation support is equipped with specificity and captures The capture agent of target target molecule；Wherein, the side of fixation support is detachably connected an airtight environment, which has Air pressure adjustable function；By adjusting the air pressure of the airtight environment, liquid sample is moved back and forth in fixation support but do not depart from The fixation support.
Wherein, capture agent is determined according to the species of target target molecule.The solvent species of liquid sample is also not limited, Including but not limited to water, ethanol equal solvent.Concrete structure is also not limited possessed by airtight environment, includes but not limited to box The shape such as shape, column, cup-shaped, spherical.Air pressure adjustable function can be by including but not limited to using syringe, peristaltic pump, air pressure The pneumatic elements such as meter, pressure valve are communicated in inside airtight environment to realize.
Wherein, fixation support preferably has the space structure of layer stereo micropore, by controlling the airtight environment Air pressure, makes liquid sample iterate through the space structure of layer stereo micropore.
Wherein, fixation support is preferably disposed to：When liquid sample is added dropwise in fixation support, in normal atmosphere (An) Under the conditions of, only relying on gravity does not make liquid sample that hanging drop occur.The purpose so set is control fixation support to liquid Adsorption capacity, fixation support can not be hung in negative pressure to avoid liquid.
Wherein, airtight environment is preferably disposed to not interfere moving about repeatedly for liquid sample.The purpose so set be for When liquid is hung to when not departing from ultimate range that fixation support can allow, structure can be outstanding possessed by airtight environment The drop of extension reserves enough spaces.
Wherein, fixation support is preferably disposed to the combination for not interfering target target molecule and capture agent.Immobilization carries The material of body may include but be not limited to nitrocellulose, Kynoar, nylon material and natural material (such as cotton, silk, Chitosan, alginic acid, starch and other poly- polysaccharide ...) or semi-synthetic (natural material and poly hydroxy ethyl acrylate (hydroxyethyl methacrylate, PHEMA), polyethylene glycol, polymethyl methacrylate (polymethyl Methacrylate, PMMA), the combination and collocation of the synthetic material such as polyvinyl alcohol (polyvinyl alcohol, PVA)) Material, these materials are respectively provided with the space structure of layer stereo micropore.The form of fixation support include but not limited to membrane type, The form such as pillar, cotton-shaped.The pore size of fixation support is arranged to allow the unbonded material in liquid sample to pass through.
Fig. 1 shows the concrete form of an airtight environment and fixation support, sees Fig. 1, and fixation support 2 is membrane type, it has There are opposite first surface 2a (lower surface) and second surface 2b (upper surface)；Airtight environment 1 is cup-shaped, and fixation support 2 can Dismantling connection is in the opening of airtight environment 1, by introducing a snorkel 3 come pneumatic elements such as external peristaltic pumps.Using When, liquid sample is added dropwise in the top of fixation support 2, is then evacuated repeatedly to airtight environment 1 by snorkel 3 and air-blowing, Control pumping and the intensity of air-blowing, achieve the purpose that to regulate and control the intensity of pressure in airtight environment 1.When this occurs, the position of drop 4a Put as shown in Fig. 2, liquid sample hanging drop (suspension) is in the first surface 2a of fixation support 2 at this time；When air-blowing, drop 4b's Position is as shown in figure 3, liquid sample floats on the second surface 2b of fixation support 2 at this time；By being evacuated repeatedly and air-blowing Target target molecule can iterate through fixation support 2 and produce specific reaction with the capture agent in fixation support 2, so that Increase the combination probability of the two.In addition, the design is since fixation support 2 being maked somebody a mere figurehead so that is easy to elute impurity, favorably It is cleaner and thorough in the matter detergent of non-specific binding is obtained, so as to reduce background interference.
Embodiment 1：Hepatitis B surface antigen is detected using airtight vial-type double antibody sandwich method
Fixation support uses nitrocellulose filter (NC films)：Whatman 0.45um
1. being 5mg/ml by capture antibody concentration, 2 μ L points are taken to be added on NC films, 37 degree of baking oven 30min are fully dry, complete Coating.
2. assembling：Order according to silicagel pad+NC films+silicagel pad assembles, and the size of silicagel pad is (straight for 12 × 1.5 × 4 Footpath is 12mm, thickness 1.5mm, a diameter of 4mm of middle perforate), silicagel pad band water resistant adhesive；NC films are cut into diameter For the circle of 7mm, it is clipped between 2 layers of silicagel pad.The NC films assembled are put into head space screw lid and compresses and is revolved with receiving flask Tightly, the structure of air-tightness is formed, it is stand-by.
3. sample-adding
By 2 times of positive plasma samples being serially diluted (containing hepatitis B surface antigen), totally 10 dilution factors, with each dilution The amount for spending 2 μ L is added separately to be coated with the NC films of capture antibody, is incubated at room temperature 2min.
4. pressure management
The use of disposable syringe is pressure control device in the present embodiment, syringe needle is stretched into receiving flask through silicagel pad In, by twitching syringe back and forth 5 times, make to produce positive/negative-pressure alternating in receiving flask, so that the sample on NC films flows repeatedly By greatly increasing the combination probability of hepatitis B surface antigen and capture antibody in sample to be checked.
5. washing
Pull syringe to make the state for keeping negative pressure in receiving flask, PBST is slowly dropped on NC films, in the work of negative pressure With lower by NC films, have the function that the uncombined albumen of washing and impurity.Washing is 100 μ L every time, takes syringe after washing Under so that the external and internal pressure of receiving flask keeps balance.
6. the indicative function of gold medal mark secondary antibody
Colloid gold particle by the labeling of monoclonal antibody of anti-hepatitis B surface antigen, is added drop-wise on 10 μ L NC films, room temperature 2min is incubated, syringe is then slowly twitched and causes colloidal gold to slow transit through NC films.Can judging result by naked eyes.Hepatitis B table The sample colloidal gold of face antigen positive will be adsorbed in NC film surfaces, the spot of the visible purple powder of naked eyes；The sample of hepatitis B surface feminine gender This by be script NC white background color.
7. testing result：The sensitivity of the detection hepatitis B surface antigen of the airtight vial-type double antibody sandwich method of the present invention is reachable To 2-8Dilution factor.
Comparative example 1a (enzyme linked immunosorbent assay)
1. kit is taken out from cold storage environment, used after putting equilibrium at room temperature 30 minutes.
2. washing lotion distilled water will be concentrated or 20 times of dilutions of deionized water are spare.
3. setting 1 hole of blank control, sample and enzyme conjugates are not added with.2 times of positive plasma samples being serially diluted are (containing hepatitis B Surface antigen), totally 10 dilution factors, are separately added into reacting hole with the amount of each 50 μ L of dilution factor.
4. adding 50 μ L of enzyme conjugates per hole, sealing plate film is capped on reaction plate, vibration mixes, and puts 37 DEG C of incubators or water-bath Reacted 60 minutes in pot.
5. discarding reaction solution, the 300 μ L of washing lotion after dilution are added per hole, 10 seconds is stood, gets rid of and abandon washing lotion, clapped after being repeated 5 times It is dry.
6. adding substrate A, each 50 μ L of B liquid per hole, sealing plate film is capped, vibration mixes, and 37 DEG C of lucifuges develop the color 15 minutes.
7. adding each 50 μ L of terminate liquid per hole, vibration, which mixes, terminates reaction.
8. being returned to zero with blank control wells, and each hole OD values are measured with microplate reader 450nm as early as possible.Also dual wavelength 450nm/ can be used 630-690nm measures each hole OD values (dual wavelength measure does not have to set blank).
9. testing result：The sensitivity of the detection hepatitis B surface antigen of enzyme linked immunosorbent assay can reach 2-8Dilution factor.
Comparative example 1b (Gold standard)
1. sealing bucket is opened, after taking out required amount of test strips, sealing bucket is shut immediately.
2. test strips are placed on horizontal on horizontal table and carry out sample labeling.
3.2 times of positive plasma samples (containing hepatitis B surface antigen) being serially diluted, totally 10 dilution factors, are inhaled with capillary Take 40 μ L samples (serum or blood plasma then 80 μ L) drop that 1 drop (40~50 is added dropwise in sample pad immediately in the sample pad of test strips μ L) sample diluting liquid (sample diluting liquid need not be then added dropwise in serum or plasma specimen).
Observed in 4.30 minutes and record experimental result.
5. testing result：The sensitivity of the detection hepatitis B surface antigen of Gold standard is 2-4Dilution factor.
By embodiment 1, comparative example 1a and 1b, from the point of view of the time that detection expends, the detection time of the method for the present invention is bright It is aobvious to be less than enzyme linked immunosorbent assay, it is close with Gold standard；From the point of view of the sensitivity of detection, the sensitivity of method of the invention is marked apparently higher than gold Method, and it is close with the sensitivity of enzyme linked immunosorbent assay.In general, there is height at the same time using the method detection hepatitis B surface antigen of the present invention Sensitivity and seldom detection time, and the sample detection amount of method of the present invention only needs 2-5 μ L, hence it is evident that less than enzyme linked immunosorbent assay and Gold standard.
Embodiment 2：Syphilis antibody is detected using 24 orifice-plate type double antibody sandwich methods
Carrier uses nitrocellulose filter (NC films)：Whatman 0.45um
1. being 5mg/ml by capture antigen concentration, 2 μ L points are taken to be added on NC films, 37 degree of baking oven 30min are fully dry, complete Coating.
2. assembling：Order according to silica gel plug+NC films+silicagel pad assembles, and the size of silica gel plug is 17 × 5 × 4 (diameters For 17mm, thickness 5mm, a diameter of 4mm of middle perforate), the size of silicagel pad is 16*0.5*4 (a diameter of 16mm, thickness For 0.5mm, a diameter of 4mm of middle perforate) silicagel pad band water resistant adhesive；NC films are cut into the circle of a diameter of 7mm, folder Between silica gel plug and silicagel pad.The structure assembled is filled in 24 orifice plates and forms the structure of air-tightness, it is stand-by.
3. sample-adding
The positive sample (containing syphilis antibody) that 2 times are serially diluted, totally 10 dilution factors, with each 2 μ L's of dilution factor Amount is added separately to be coated with the NC films of capture antibody, is incubated at room temperature 2min.
4. pressure management
The use of peristaltic pump is pressure control device in the application example, syringe needle stretched into through silicagel pad in 24 orifice plates and with wriggling Pump is connected, and controls the positive/negative-pressure in 24 orifice plates to replace by the rotation direction of peristaltic pump, so that the sample on NC films is repeatedly Flow through, greatly increase sample to be checked with capturing the combination probability of antigen and antibody to be checked.
5. washing
Start peristaltic pump and maintain the state of negative pressure, PBST is slowly dropped on NC films, is passed through under the action of negative pressure NC films, have the function that the uncombined albumen of washing and impurity.Washing is 100 μ L every time, and peristaltic pump is closed after washing so that is collected The external and internal pressure of bottle keeps balance.
The addition of the Lues Assay antigen of 6.HRP marks
By the Treponema pallidum antigen of HRP marks with 1:8000 concentration, is added drop-wise on 10 μ L NC films, is incubated at room temperature 2min, then Opening peristaltic pump causes solution to iterate through NC films.
7. washing
Start peristaltic pump and maintain the state of negative pressure, PBST is slowly dropped on NC films, is passed through under the action of negative pressure NC films, have the function that the uncombined albumen of washing and impurity.Washing is 100 μ L every time, and peristaltic pump is closed after washing so that is collected The external and internal pressure of bottle keeps balance.
8. sedimentation type TMB develops the color
Sedimentation type TMB chromogenic substrates are added drop-wise on NC films, the syphilis antibody positive will form bluish violet in NC film surfaces Spot；The sample of syphilis antibody feminine gender by be script NC white background color.
9. instrument takes pictures and scans the gray scale of post analysis spot, so as to fulfill the measure syphilis antibody value of sxemiquantitative.
10. testing result：The sensitivity of 24 orifice-plate type double antibody sandwich methods detection syphilis antibody can reach 2-4。
Comparative example 2a (enzyme linked immunosorbent assay)
1. balance：Kit is taken out from cold storage environment, is used after putting equilibrium at room temperature 30 minutes.
2. match somebody with somebody liquid：Washing lotion distilled water will be concentrated or 20 times of dilutions of deionized water are spare.
3. setting：Stay a hole to make blank control, any liquid wouldn't be added.Separately set 3 hole of negative control, positive control does 2 times It is serially diluted, totally 10 dilution factors, is separately added into the amount of each 100 μ L of dilution factor in 96 orifice plates.
4. incubate：Sealing plate film is capped on reaction plate, vibration is mixed, put in 37 DEG C of incubators or water-bath, is reacted 30 minutes.
5. board-washing：Reaction solution is discarded, the 300 μ L of washing lotion after dilution are added per hole, is soaked 15 seconds, is got rid of and abandon washing lotion.Continuously wash Plate 5 times, finally pats dry.
It is 6. enzyme：100 μ L enzyme conjugates are added per hole (blank control wells are not added with).
7. incubate：Sealing plate film is capped on reaction plate, is put in 37 DEG C of incubators or water-bath, is reacted 30 minutes.
8. board-washing：Board-washing 5 times, biconditional operation step 6.
9. colour developing：Each 50 μ L (including blank control wells) of substrate A, B liquid are sequentially added per hole, are capped sealing plate film, vibration is mixed Even, 37 DEG C of lucifuges develop the color 10 minutes.
10. terminate：Each 50 μ L (including blank control wells) of terminate liquid are added per hole, vibration, which mixes, terminates reaction.
11. measure：Returned to zero with blank control wells, and in using microplate reader Single wavelength 450nm to measure each hole OD values in 30 minutes. Also dual wavelength 450nm/630nm can be used to measure each hole OD values.
12. testing result：The sensitivity of enzyme linked immunosorbent assay detection syphilis antibody can reach 2-4。
Comparative example 2b (Gold standard)
1. sealing bucket is opened, after taking out required amount of test strips, sealing bucket is shut immediately.
2. test strips are placed on horizontal on horizontal table and carry out sample labeling.
3.2 times of positive plasma samples (containing syphilis antibody) being serially diluted, totally 10 dilution factors, 40 are drawn with capillary 1 drop (40~50 μ L) is added dropwise in the sample pad of test strips in μ L samples (serum or blood plasma then 80 μ L) drop in sample pad immediately Sample diluting liquid (sample diluting liquid need not be then added dropwise in serum or plasma specimen).
Observed in 4.30 minutes and record experimental result.
5. testing result：The sensitivity of Gold labeled method syphilis antibody is 2-2。
Pass through embodiment 2, comparative example 2a and 2b, from the point of view of the time that detection expends, the detection time of method of the invention Considerably less than enzyme linked immunosorbent assay is close with Gold standard；From the point of view of the sensitivity of detection, the sensitivity of method of the invention is apparently higher than gold Mark method, and it is close with the sensitivity of enzyme linked immunosorbent assay.In general, there is Gao Ling at the same time using the method detection syphilis antibody of the present invention Sensitivity and seldom detection time, and the sample detection amount of the method for the present invention only needs 2-5 μ L, hence it is evident that less than enzyme linked immunosorbent assay and gold Mark method.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art Realize other modification, therefore under the universal limited without departing substantially from claim and equivalency range, it is of the invention and unlimited In specific details and shown here as the legend with description.

Claims (6)

1. a kind of target target molecule quick capturing method for in-vitro diagnosis, it is characterised in that the target target molecule is present in In liquid sample, which is added dropwise on fixation support, the fixation support is equipped with specificity capture target The capture agent of target molecule；
Wherein, the side of the fixation support is detachably connected an airtight environment, which has air pressure adjustable function；
By adjusting the air pressure of the airtight environment, liquid sample is moved back and forth in the fixation support but do not depart from this and fix Change carrier.
2. the target target molecule quick capturing method according to claim 1 for in-vitro diagnosis, it is characterised in that described Fixation support has the space structure of layer stereo micropore, by controlling the air pressure of the airtight environment, makes liquid sample past Pass through the layer stereo micropore in the fixation support again.
3. the target target molecule quick capturing method according to claim 1 for in-vitro diagnosis, it is characterised in that described Fixation support is arranged to：When liquid sample is added dropwise in fixation support, under normal atmospheric conditions, gravity is only relied on Do not make liquid sample that hanging drop occur.
4. the target target molecule quick capturing method according to claim 1 for in-vitro diagnosis, it is characterised in that described Airtight environment is arranged to the reciprocating motion for not interfering liquid sample.
5. the target target molecule quick capturing method according to claim 1 for in-vitro diagnosis, it is characterised in that described Fixation support is arranged to the combination for not interfering target target molecule and capture agent.
6. the target target molecule quick capturing method according to claim 1 for in-vitro diagnosis, it is characterised in that described The form of fixation support is arranged to membrane type, pillar or cotton-shaped.
CN201711158456.0A 2017-11-20 2017-11-20 A kind of target target molecule quick capturing method for in-vitro diagnosis Pending CN107966558A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021169089A1 (en) * 2020-02-25 2021-09-02 中山大学 Microfluidic chip-based rapid immunoassay method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001019517A1 (en) * 1999-09-14 2001-03-22 Pamgene, B.V. Analytical test device with substrate having oriented through going channels and improved methods and apparatus for using same
CN101421041A (en) * 2006-04-14 2009-04-29 皇家飞利浦电子股份有限公司 Foam inhibitor membrane for a flow-through cell

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001019517A1 (en) * 1999-09-14 2001-03-22 Pamgene, B.V. Analytical test device with substrate having oriented through going channels and improved methods and apparatus for using same
CN101421041A (en) * 2006-04-14 2009-04-29 皇家飞利浦电子股份有限公司 Foam inhibitor membrane for a flow-through cell

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021169089A1 (en) * 2020-02-25 2021-09-02 中山大学 Microfluidic chip-based rapid immunoassay method

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