CN107961284A - A kind of oral formulations for preventing grice diarrhoea and preparation method thereof - Google Patents
A kind of oral formulations for preventing grice diarrhoea and preparation method thereof Download PDFInfo
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/739—Sanguisorba (burnet)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The invention discloses a kind of oral formulations for preventing grice diarrhoea and preparation method thereof, the present invention is prepared by nutgall extractive, Purslane extract, garden burnet extracting solution, flavouring, preservative and reducing agent, the oral formulations are through associated production practice and veterinary clinic experiment results proved, grice diarrhoea can effectively be prevented and treated, the quantity and the death rate for even eliminating sick pig pathogenic bacteria are reduced, while also there is volume increase.Present invention process is simple, has that wide spectrum, antimicrbial power are strong, are not likely to produce the small effect of drug resistance, toxic side effect, and better stability of preparation, cost are reasonable, greatly improve work efficiency.
Description
Technical field
The invention belongs to field of veterinary, and in particular to a kind of oral formulations for preventing grice diarrhoea and preparation method thereof.
Background technology
Grice diarrhoea, which all has piglet survival ratio and later stage weightening, to be seriously affected, and is become and is restricted the important of pig breeding industry development
Disease.It is reported that diarrhea accounts for the 35 ~ 45% of swine disease total amount, it is the relatively conventional disease of pig breeding industry, grice diarrhoea is more almost
The problem of will running into of each farm.It is very common that diarrhea phenomenon occurs within 1 week after wean, the death rate often up to 10% ~
20%, it is high up to more than 40%.It is rapid to treat the antibiosis class drug development of piglet diarrhoea, and is widely used in veterinary clinic,
The dosage used is also increasing, and antibody-resistant bacterium also gradually increases, and to clinical treatment, the disease causes many unfavorable factors, caused
Food security, environmental pollution and drug resistance problems are of increasing concern.Many developed countries successively forbid making using antibiotic
For feed addictive, while cut down and even stopped from China's import meat using green barrier, made to the outlet of China's meat products
Into massive losses.
Existing antidiarrheal for animals, content are mostly antimicrobial and probiotics, and antimicrobial clinically wide
General use causes drug resistance and adverse reaction to increase year by year, easily causes serious suprainfection and other drug induced diseases.With
Taking place frequently for food-safety problem in recent years, more and more people favor Chinese medicines and the disease of herding class animal is treated.
Active material contained by Chinese medicine compound prescription has Mutiple Targets, can strengthen human body immune function, improves animal resisting stress, anti-disease energy
Power, improves breeding performonce fo animals, and has the advantages that low-residual, is not likely to produce drug resistance.Therefore it is young to develop treatment wean
Diarrhea of pigs Chinese medicine compound prescription has great importance the pig breeding industry in China.Chinese medicine antibacterial spectrum is wide, pharmacological activity is good and medicament residue
Problem is not serious, can fundamentally solve a series of problems that animal husbandry is frequently brought using antibiotic.
Based on above-mentioned analysis, a kind of antibiotic property is wide, antimicrbial power is strong, the small, preparation stabilization that is not likely to produce drug resistance, toxic side effect
Property it is good, it is of reasonable cost prevention porkling diarrhea oral formulations be that the industry is badly in need of at present.
The content of the invention
In view of above-mentioned deficiency, it is an object of the invention to provide a kind of antibiotic property is wide, antimicrbial power is strong, is not likely to produce drug resistance
Property, toxic side effect is small, oral formulations of better stability of preparation, prevention porkling diarrhea of reasonable cost and preparation method thereof.
In order to achieve the above object, present invention employs following technical scheme, the dosage of each component of the present invention is also process
Inventor carries out largely groping to summarize what is drawn, and the oral formulations that each component dosage is prepared in the range of following weight are respectively provided with
Antibiotic property is wide, antimicrbial power is strong, be not likely to produce drug resistance, toxic side effect is small, better stability of preparation, advantage of reasonable cost.
A kind of oral formulations for preventing grice diarrhoea are made of the raw material of following weight：
10 ~ 20 parts of nutgall extractive, 2 ~ 4 parts of Purslane extract, 2 ~ 4 parts of garden burnet extracting solution, 3 ~ 5 parts of flavouring, preservative
0.2 ~ 0.4 part, 0.1 ~ 0.3 part of reducing agent.
Further, which further includes the raw material of following weight：
2 ~ 4 parts of Purslane extract, 2 ~ 4 parts of garden burnet extracting solution.
Further, which is made of the raw material of following weight：
15 parts of nutgall extractive, 3 parts of Purslane extract, 3 parts of garden burnet extracting solution, 4 parts of flavouring, 0.3 part of preservative, reduction
0.2 part of agent.
Further, the flavouring is sucrose.
Further, the preservative be by mass ratio be 5 ~ 3:1 benzoic acid：Ethylparaben mixes.
Further, the reducing agent is sodium pyrosulfite.
Further, the nutgall extractive is made by following preparation method：Take Chinese gall to cross 20 mesh sieves after crushing to obtain
Coarse powder, coarse powder are merged extracting solution, are then used extracting solution with the 10-20 times of distillation measured water boiling and extraction 2-3 times, each 2-3h
1-2 times of volume of chloroform continuous extraction 2-3 times, collects water phase；Then the ethyl acetate of 2-3 times of volume is added, 45 ± 5 DEG C of shaking tables shake
30-40min is swung, stratification, collects ethyl acetate phase, and after 40 DEG C of decompression rotary evaporation recycling ethyl acetate, freezing is dry
It is dry, obtain nutgall extractive.
Further, the Purslane extract is made by following preparation method：Take purslane dry and crush, use
95% ethanol is as entrainment reagent, CO2Supercritical extract, extract addition 1,3 ~ butanediol, organic layer reclaim reagent to most, drying
Extract A is obtained, after above-mentioned residue is collected plus when 5 times of amount water decoctions 1 are small, filtering, filtrate decompression is concentrated into relative density and is
1.1 ~ 1.2, extract B is obtained, extract A and extract B are uniformly mixed, obtains Purslane extract.
Further, the garden burnet extracting solution is made by following preparation method：2 purifications are carried out to garden burnet using ethanol,
After merging extracting solution, pH to 10 ~ 15 is adjusted, filtrate is obtained after centrifugation, filtrate is adsorbed using macroporous absorbent resin, then
Eluted respectively using ethanol and organic solvent, collect organic solvent eluent, and concentrated, stand, filter, collect sediment, obtained
To garden burnet extracting solution.
Present invention also offers a kind of preparation method for the oral formulations for preventing grice diarrhoea：（1）Take distilled water to boil to remove
Oxygen, then stands cooling 1h, obtains deoxygenation distilled water；（2）Nutgall extractive, Purslane extract, garden burnet are measured by formula to carry
Taking liquid to add in appropriate deoxygenation distilled water, add flavouring, preservative and reducing agent, stirring is abundant, deoxygenation distilled water constant volume,
The oral formulations of grice diarrhoea must be prevented.The oral formulations of obtained prevention grice diarrhoea, said preparation color are brown color, color
Pool is clear and bright, and content of tannin is more than 45%.
The beneficial effect of this hair is：
1st, oral formulations of the invention are to humans and animals and Environmental security, and preparation process is simple, convenient drug administration, lasting medicine, with
Achieve the purpose that to treat grice diarrhoea.
2nd, the present invention compensate for conventional antibiotic medicament residue and drug resistance problems caused by grice diarrhoea, for piglet
Diarrhea effect has the advantages such as certain effect, noresidue and drug resistance.
3rd, it is uniformly dispersed through solution produced by the present invention, main ingredient, stability is good, and convenient drug administration, holding effect are long, toxic side effect
It is small, meet Chinese Pharmacopoeia requirement, it is quality controllable.
4th, the present invention shows there is prevention and treatment for manual-induced secretory diarrhea mouse model through pharmacodynamic study
Effect.
5th, formula of the invention and technique are inseparable, mutually collaboration, are an indispensable integrated artistics, only exist
Under inventive formulation and process conditions, each raw material could be balanced well, shown that stability is good, be uniformly dispersed, convenient drug administration, medicine
Effect is lasting, the oral formulations of the small prevention grice diarrhoea of toxic side effect.
Embodiment
We will the invention will be further elaborated with reference to embodiment below.
Embodiment 1
A kind of oral formulations for preventing grice diarrhoea
（1）Prepare nutgall extractive：Take Chinese gall to cross 20 mesh sieves after crushing and obtain coarse powder, coarse powder is decocted with the distilled water of 15 times of amounts
Extraction 2 times, each 2h, merges extracting solution, then by extracting solution monoploid product chloroform continuous extraction 2 times, collects water phase；Then
The ethyl acetate of diploid product is added, 45 DEG C of shaking tables vibrate 30min, and stratification, collects ethyl acetate phase, then at 40 DEG C of decompressions
After rotary evaporation recycling ethyl acetate, freeze-drying, obtains nutgall extractive.
（2）Prepare Purslane extract：Take purslane dry and crush, using 95% ethanol as entrainment reagent, CO2It is super to face
Boundary extracts, and extract adds 1, and 3 ~ butanediol, organic layer reclaim reagent is dried to obtain extract A, above-mentioned residue is collected to the greatest extent
When afterwards plus 5 times of amount water decoctions 1 are small, filtering, it is 1.1 ~ 1.2 that filtrate decompression, which is concentrated into relative density, obtains extract B, will extract
Thing A and extract B are uniformly mixed, and obtain Purslane extract；
（3）Prepare garden burnet extracting solution：Garden burnet is taken to impregnate 90min with 60% ethanol solution of 10 times of weight, in 40 DEG C of condition
Under to be filtered after the power ultrasonic of 3000W extraction 30min；Filter residue is again with 60% ethanol solution ultrasonic extraction of 10 times of weight, bar
Part is same as above, filtering, and filtrate is spare twice for merging, and it is 1.1 ~ 1.2 that filtrate, which is concentrated into relative density, obtains garden burnet extracting solution；
（4）Prepare oral formulations：Take distilled water to boil deoxygenation, then stand cooling 1h, obtain deoxygenation distilled water；Chinese gall is taken to extract
Thing 15kg, Purslane extract 3kg, garden burnet extracting solution 3kg are added in appropriate deoxygenation distilled water, and stirring is abundant, then adds sugarcane
Sugared 4kg, benzoic acid 0.25kg, ethylparaben 0.05kg, sodium pyrosulfite 0.2kg, constant volume is filling, obtains finished color and is
Brown color, color and luster is clear and bright, the oral formulations of prevention grice diarrhoea of the nutgall extractive content more than 45%.
Embodiment 2
A kind of oral formulations for preventing grice diarrhoea
Preparation method is that the oral formulations each component content is with embodiment 1：Nutgall extractive 10kg, purslane extraction
Liquid 2kg, garden burnet extracting solution 2kg, sucrose 3kg, benzoic acid 0.15kg, ethylparaben 0.05kg, sodium pyrosulfite 0.1kg, with
It is brown color that color, which is made, in appropriate deoxygenation distilled water, and color and luster is clear and bright, and nutgall extractive content is more than 45% prevention grice diarrhoea
Oral formulations.
Embodiment 3
A kind of oral formulations for preventing grice diarrhoea
Preparation method is that the oral formulations each component content is with embodiment 1：Nutgall extractive 20kg, purslane extraction
Liquid 4kg, garden burnet extracting solution 4kg, sucrose 5kg, benzoic acid 0.3kg, ethylparaben 0.1kg, sodium pyrosulfite 0.3kg, with fitting
It is brown color that color, which is made, in amount deoxygenation distilled water, and color and luster is clear and bright, prevention grice diarrhoea of the nutgall extractive content more than 45%
Oral formulations.
Comparative example 1
Preparation method is free of Purslane extract and garden burnet extracting solution with embodiment 1 in formulation of simply withdrawing the previous remark.
Simply the oral formulations each component content is：Nutgall extractive 20kg, Purslane extract 4kg, garden burnet extraction
Liquid 4kg, sucrose 5kg, benzoic acid 0.3kg, ethylparaben 0.1kg, sodium pyrosulfite 0.3kg, with appropriate deoxygenation distilled water system
It is brown color into color, color and luster is clear and bright, the oral formulations of prevention grice diarrhoea of the nutgall extractive content more than 45%.
Comparative example 2
Preparation method is simply free of garden burnet extracting solution with embodiment 1 in the oral formulations.
Comparative example 3
Preparation method is free of Purslane extract with embodiment 1 in formulation of simply withdrawing the previous remark.
A kind of effect for the oral formulations In Vitro Bacteriostasis for preventing grice diarrhoea
Chinese gall oral solution prepared by Example 1, carries out body for the Escherichia coli of four kinds of different serotypes respectively
There is certain restraining and sterilizing bacteria to act on, refer to table 1. for outer drug effect, explanation Chinese gall oral solution of obtaining a result
1 In Vitro Bacteriostasis result of table（mg/ml）
。
Note：MIC：Minimal inhibitory concentration；MBC：Minimum bactericidal concentration.All of above bacterial strain bacterium is Escherichia coli, wherein
O101, O78（Standard）, O6 is reference culture, O78（Pig source）To be clinically separated swine Escherichia coli.
The oral formulations for preventing grice diarrhoea evaluate effect test to the resistivity of diarrhea caused by castor oil
Chinese gall oral formulations prepared by Example 1, comparative example 1-3, select 30 ICR male mices（Weight 18 ~
22g）, 6 groups are randomly divided into, every group 5, fasting for solids but not liquids 18h before experiment, the 1st group is blank control group, without any processing；
2-6 groups are test group, wherein the 2nd group is negative control group, the physiological saline for giving 10ml/kg gavages；3rd group is embodiment 1
Group, 1 preparation of embodiment for giving 900mg/kg gavage；4th ~ 6 group is test group, gavage respectively comparative example 1, comparative example 2 and
Chinese gall oral formulations 900mg/kg obtained by comparative example 3.The pretreatment of 1h is carried out to 1 ~ 6 group of mouse, then to 2 ~ 6 groups
The castor oil of mouse gavaging 10ml/kg, and mouse is placed on clean newspaper, observes in 4h diarrhea of mouse frequency and fecal
Shape, and it is as follows according to resistivity of the related evaluation criteria to mouse to diarrhea caused by castor oil, Score index：0 point：Without abdomen
Rush down；1 point：Soft and shaping excrement；2 points：Very soft excrement；3 points：Watery stools.Individual, which is scored at, it is expected that score value is multiplied by excrement
Just quantity, every group of overall scores are the sum of individual score.Diarrhea reduced rate calculation formula is as follows：
Diarrhea reduced rate=（Negative control group average-test group average）/ negative control group average × 100%
To resistivity evaluation result such as 2 ~ table of table 4 of diarrhea caused by castor oil：The anti-diarrhea effect of each group Chinese gall oral formulations.
2 each group diarrhea average mark of table
。
3 each group loose stool rate of table（%）
。
4 each group diarrhea reduced rate of table（%）
。
According to table 2-3, it can be clearly seen that relative to negative control group, test the diarrhea average mark, dilute of each group
Just rate is substantially less than negative control group, and contains compound formula in embodiment 1（Nutgall extractive+Purslane extract and
Garden burnet extracting solution）Oral formulations diarrhea average mark, loose stool rate be significantly lower than comparative example 1（Nutgall extractive folk prescription）、
Comparative example 2（Nutgall extractive+Purslane extract）, comparative example 3（Nutgall extractive+garden burnet extracting solution）；Equally, according to
Shown in table 4, compound formula is contained in embodiment 1（Nutgall extractive+Purslane extract and garden burnet extracting solution）Oral system
Agent diarrhea reduced rate is apparently higher than comparative example 1（Nutgall extractive folk prescription）, comparative example 2（Nutgall extractive+purslane extraction
Liquid）, comparative example 3（Nutgall extractive+garden burnet extracting solution）；As it can be seen that Purslane extract, garden burnet extracting solution are carried for Chinese gall
Take thing that there is obvious synergistic function in prevention diarrhea this respect.
The oral formulations for preventing grice diarrhoea test the situation of change of diarrhea small intestine enteron aisle hydrops caused by castor oil
ICR male mices are grouped and pretreatment is the same as resistivity of the oral formulations to diarrhea caused by castor oil for preventing grice diarrhoea
Effect test is evaluated, in addition to the 1st group, all animals gavage 10ml/kg castor oil, after 4h, put to death mouse with folding cervical approach, weigh
And weight is recorded, excision duodenum to whole section of small intestine of rectum, avoids hydrops from leaking out, and weighs after removing mesenterium, use is dirty
Device index is as hydrops foundation：
Organ index=every group of average small intestine weight/every group of average mice body weight × 100%
To the situation of change such as table 5 of diarrhea small intestine enteron aisle hydrops caused by castor oil.
5 small intestine hydrops rate of table（%）
。
Small intestine hydrops situation of change determines that organ index situation is as shown in table 5, and numerical value is bigger according to organ index index
Illustrate that hydrops is more, compareed as can be seen from Table 5 with negative control group, 1 group of embodiment, 1 group of comparative example, 2 groups of comparative example, contrast
3 groups of example all has certain inhibitory action to small intestine hydrops；But contain compound formula in embodiment 1（Nutgall extractive+dent
Amaranth extracting solution and garden burnet extracting solution）Oral formulations small intestine hydrops rate be significantly lower than comparative example 1（Nutgall extractive folk prescription）, it is right
Ratio 2（Nutgall extractive+Purslane extract）, comparative example 3（Nutgall extractive+garden burnet extracting solution）；As it can be seen that purslane
Extracting solution, garden burnet extracting solution have obvious synergistic function for nutgall extractive in suppression small intestine hydrops this respect.
The oral formulations for preventing grice diarrhoea test influence of the diarrhea caused by castor oil to gastrointestinal motility
ICR male mices are grouped and pre-process the same oral formulations for preventing grice diarrhoea to diarrhea caused by castor oil to gastrointestinal motility
Influence experiment, in addition to the 1st group, all animals gavage 10ml/kg castor oil, and after 1h, every mouse gavaging 0.3ml powdered carbon mixes
Suspension（10 parts of+5 parts of powdered carbon+5 parts of Arabic gum starch）, parameter is for powdered carbon in every mouse intestinal from pylorus to caecum
Distance and the ratio of small intestinal length, specific formula for calculation are as follows：
Intestinal propulsive rate=powdered carbon length/small intestinal length × 100%
It is as shown in table 6 to influence of the diarrhea caused by castor oil to gastrointestinal motility.
6 each group Intestinal propulsive rate of table（%）
。
Charcoal end advance distance percentage is as shown in table 6, is compareed with negative control group, 1 group of embodiment, 1 group of comparative example, contrast
2 groups of example, 3 groups of comparative example all have certain inhibitory action for gastrointestinal motility；Wherein, compound formula is contained in embodiment 1（Five
Times seed extract+Purslane extract and garden burnet extracting solution）The Intestinal propulsive rates of oral formulations be significantly lower than comparative example 1（Five
Times seed extract folk prescription）, comparative example 2（Nutgall extractive+Purslane extract）, comparative example 3（Nutgall extractive+garden burnet
Extracting solution）；As it can be seen that Purslane extract, garden burnet extracting solution are directed to nutgall extractive in terms of gastrointestinal motility is suppressed with bright
Aobvious synergistic function.
Claims (9)
1. it is a kind of prevent grice diarrhoea oral formulations, it is characterised in that the oral formulations by following weight raw material
It is made：10 ~ 20 parts of nutgall extractive, 3 ~ 5 parts of flavouring, 0.2 ~ 0.4 part of preservative, 0.1 ~ 0.3 part of reducing agent.
2. it is according to claim 1 prevention grice diarrhoea oral formulations, it is characterised in that the oral formulations further include as
The raw material of lower weight：2 ~ 4 parts of Purslane extract, 2 ~ 4 parts of garden burnet extracting solution.
3. the oral formulations of prevention grice diarrhoea according to claim 2, it is characterised in that the oral formulations by weighing as follows
The raw material of amount part proportioning is made：15 parts of nutgall extractive, 3 parts of Purslane extract, 3 parts of garden burnet extracting solution, 4 parts of flavouring,
0.3 part of preservative, 0.2 part of reducing agent.
4. the oral formulations of the prevention grice diarrhoea according to claim 1 or 3, it is characterised in that the flavouring is sugarcane
Sugar, the preservative be by mass ratio be 5 ~ 3:1 benzoic acid：Ethylparaben mixes, and the reducing agent is burnt sulfurous
Sour sodium.
5. the oral formulations of the prevention grice diarrhoea according to claim 1-3 any one, it is characterised in that described five times
Seed extract is made by following methods：Take Chinese gall to cross 20 mesh sieves after crushing and obtain coarse powder, coarse powder is with the 10-20 times of distillation measured decocting
Extraction 2-3 time, each 2-3h is boiled, merges extracting solution, then by extracting solution 1-2 times of volume of chloroform continuous extraction 2-3 times, collection
Water phase；Then the ethyl acetate of 2-3 times of volume is added, 45 ± 5 DEG C of shaking tables vibrate 30-40min, and stratification, collects acetic acid second
Ester phase, after 40 DEG C of decompression rotary evaporation recycling ethyl acetate, freeze-drying, obtains nutgall extractive.
6. the oral formulations of the prevention grice diarrhoea according to claim 1-3 any one, it is characterised in that the dent
Amaranth extracting solution is made by following methods：Take purslane dry and crush, using 95% ethanol as entrainment reagent, CO2Overcritical extraction
Take, extract adds 1, and 3 ~ butanediol, organic layer reclaim reagent is dried to obtain extract A to the greatest extent, adds after above-mentioned residue is collected
5 times of amount water decoct 1 it is small when, filtering, filtrate decompression is concentrated into relative density as 1.1 ~ 1.2, obtains extract B, by extract A and
Extract B is uniformly mixed, and obtains Purslane extract.
7. the oral formulations of the prevention grice diarrhoea according to claim 1-3 any one, it is characterised in that the garden burnet
Extracting solution is made by following methods：Garden burnet is taken to impregnate 60-90min with the ethanol solution of the 60-75% of 10 times of weight, in 40-45
Filtered after extracting 30-40min under conditions of DEG C with the power ultrasonic of 3000-4000W；Filter residue is again with the 60-75%'s of 10 times of weight
Ethanol solution ultrasonic extraction, condition are same as above, filtering, and filtrate is spare twice for merging, and it is 1.1 ~ 1.2 that filtrate, which is concentrated into relative density,
Obtain garden burnet extracting solution.
A kind of 8. preparation method of the oral formulations of prevention grice diarrhoea as described in claim 1 ~ 7 is any, it is characterised in that
The preparation method includes：
（1）Take distilled water to boil deoxygenation, then stand cooling 1h, obtain deoxygenation distilled water；
（2）Nutgall extractive, Purslane extract, garden burnet extracting solution are measured by formula to add in appropriate deoxygenation distilled water, then
Flavouring, preservative and reducing agent are added, stirring is abundant, deoxygenation distilled water constant volume, must prevent the oral formulations of grice diarrhoea.
9. a kind of oral formulations of prevention grice diarrhoea according to obtained by any described the method for claim 8, the system
Agent color is brown color, and color and luster is clear and bright, and content of tannin is more than 45%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711472515.1A CN107961284B (en) | 2017-12-29 | 2017-12-29 | Oral preparation for preventing and treating piglet diarrhea and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711472515.1A CN107961284B (en) | 2017-12-29 | 2017-12-29 | Oral preparation for preventing and treating piglet diarrhea and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
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| CN107961284A true CN107961284A (en) | 2018-04-27 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102894229A (en) * | 2012-10-24 | 2013-01-30 | 刘向前 | Natural feed additive with functions of relieving diarrhea, resisting bacteria and eliminating inflammation and preparation method thereof |
| CN106511438A (en) * | 2016-11-18 | 2017-03-22 | 江西正邦动物保健品有限公司 | Compound solution for preventing and treating piglet diarrhea and preparation method thereof |
| CN106822890A (en) * | 2017-01-19 | 2017-06-13 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of pharmaceutical composition for preventing and treating diarrhea of weaned piglets and its preparation method and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102894229A (en) * | 2012-10-24 | 2013-01-30 | 刘向前 | Natural feed additive with functions of relieving diarrhea, resisting bacteria and eliminating inflammation and preparation method thereof |
| CN106511438A (en) * | 2016-11-18 | 2017-03-22 | 江西正邦动物保健品有限公司 | Compound solution for preventing and treating piglet diarrhea and preparation method thereof |
| CN106822890A (en) * | 2017-01-19 | 2017-06-13 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of pharmaceutical composition for preventing and treating diarrhea of weaned piglets and its preparation method and application |
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