CN107937326A - A kind of Paenibacillus polymyxa and its application for being used to prevent American ginseng root maize ear rot - Google Patents
A kind of Paenibacillus polymyxa and its application for being used to prevent American ginseng root maize ear rot Download PDFInfo
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- CN107937326A CN107937326A CN201810042350.2A CN201810042350A CN107937326A CN 107937326 A CN107937326 A CN 107937326A CN 201810042350 A CN201810042350 A CN 201810042350A CN 107937326 A CN107937326 A CN 107937326A
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- paenibacillus polymyxa
- american ginseng
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
Abstract
Paenibacillus polymyxa and its application the present invention relates to one plant of antagonism American ginseng root maize ear rot sickle-like bacteria.Belong to the development and utilization field of technical field of plant disease biological control, microbial resources, the preserving number of the Paenibacillus polymyxa is：CGMCC No.15112.The application is application of the Paenibacillus polymyxa in antagonism sickle-like bacteria, prevention American ginseng root maize ear rot, watermelon blight, wheat scab, Solanaceae droop.Compared with existing pesticide and bacteria agent, Paenibacillus polymyxa DN67 of the invention has efficient antagonism to sickle-like bacteria.
Description
Technical field
The invention belongs to the development and utilization field of technical field of plant disease biological control, microbial resources, especially relate to
And a kind of Paenibacillus polymyxa and its application.
Background technology
American Ginseng (scientific name：Panax quinquefolius) it is Araliaceae Panax herbs perennial vegetation, alias star-spangled banner
Ginseng, American ginseng, Western ginseng, American ginseng, originate in the Wisconsin State of Canadian big Quebec and the U.S., in
Also there is plantation on the ground such as state's Beijing Huairou, Shandong Wendeng City and Changbai Mountain.American Ginseng has Yin-nourishing and Qi-supplementing, allay excitement intelligence development and heat-clearing life
Tianjin, the double effects that fall fire is relieved summer heat, is widely used, and the market demand is very big.But, American Ginseng continuous cropping obstacle is extremely serious, same
When continuously planting American Ginseng, often pest and disease damage is serious, and the underproduction is even had no harvest block.Research is found, maximum is threatened to American Ginseng
For root rot mainly caused by sickle-like bacteria (Fusarium sp.), which is also the disease of the crops such as banana, watermelon, cucumber at the same time
Opportunistic pathogen.
As the development of green agriculture theory, country are stringent all the more to the limitation of crops pesticide residue, American Ginseng plantation
Pesticide use species and scope are extremely restricted during management, to sickle-like bacteria most effective class containing pentachloronitrobenzene pesticide
It is disabled, lack specific drug when root rot occurs, heavy economic losses is brought to farmer.Exist including microbial bacterial agent
Interior biological prevention and control agent research is very much, and for the disease of other crops caused by sickle-like bacteria, the Antagonistic Fungi species of report is also very much, than
As University Of Agriculture and Forestry In Fujian reports《One plant of bacillus subtilis for being used for antagonism radix pseudostellariae specialized form Fusarium oxysporum》Obtain
Invention patent mandate, but radix pseudostellariae is pinkwort caryophyllaceous ginseng Pseudostellaria heterophylla (Miq.) Pax
Dried root, greatly differ from each other with the American Ginseng affiliation of Araliaceae, and the separated bacterial strain of this research source in contrast
Difference, strain are also different.
The content of the invention
The present invention screens one plant from the bed mud of Yantai Bohai Sea coastal waters and is used for the more viscous of antagonism American ginseng root maize ear rot sickle-like bacteria
Series bacillus.The zymotic fluid of the Paenibacillus polymyxa can efficiently suppress American ginseng root maize ear rot sickle-like bacteria, in the field
Have a extensive future.
The one plant provided by the invention Paenibacillus polymyxa for being used for antagonism American ginseng root maize ear rot sickle-like bacteria, for more viscous class bud
Spore bacillus (Paenibacillus polymyxa) DN67, on December 21st, 2017 in Chinese microorganism strain preservation management
Committee's common micro-organisms center registration preservation, deposit number are：CGMCC No.15112, preservation address：Chaoyang District, Beijing City
The institute 3 of North Star West Road 1.
The fermentation culture conditions of the Paenibacillus polymyxa DN67 are 28~37 DEG C, and the time is 18~24h.
The fermentative medium formula of the bacterium is：Glucose 1~2%, yeast extract 0.5~1.0%, dipotassium hydrogen phosphate 0.5
~1.0%, sodium chloride 0.2~0.5%, ammonium sulfate 0.5~1.0%, magnesium sulfate 0.03~0.05%, adjusting pH to 6.5~
7.5。
The present invention also provides the Paenibacillus polymyxa DN67 answering in American ginseng root maize ear rot sickle-like bacteria medicine is prevented
With.
The present invention also provides a kind of biocontrol agent containing the Paenibacillus polymyxa DN67.
Further, the concentration of Paenibacillus polymyxa DN67 described in the biocontrol agent is 1 × 108~1 × 1010CFU/
ML or 1 × 108~1 × 1010CFU/g。
The present invention also provides the preparation method of the biocontrol agent, step includes：
(1) Paenibacillus polymyxa is inoculated in fluid nutrient medium and cultivated, to the dilution of 20 times of nutrient solution after OD600
For 0.3~0.8, seed liquor is obtained；
(2) seed liquor is inoculated in fermentation medium to expand and cultivated, the cell concentration into zymotic fluid reaches 1 × 109
~1 × 1010CFU/mL, obtains the biocontrol agent.
Further, the volume ratio of the inoculum concentration of the seed liquor is 1~10%.
Further, the component and final concentration of the fluid nutrient medium:Glucose 1.6%, sodium chloride 0.25%, magnesium sulfate
0.05%, yeast extract 1%, ammonium chloride 0.7%, dipotassium hydrogen phosphate 0.1%, pH7.
Further, the formula of the fermentation medium is：Glucose 1~2%, yeast extract 0.5~1.0%, phosphoric acid hydrogen
Dipotassium 0.5~1.0%, sodium chloride 0.2~0.5%, ammonium sulfate 0.5~1.0%, magnesium sulfate 0.03~0.05%, adjust pH to
6.5~7.5.
Further, the temperature for expanding culture is 28~37 DEG C.
The beneficial effect of the present invention compared with prior art：
Before American Ginseng plantation or in planting process, Paenibacillus polymyxa bacteria suspension is uniformly sprayed on American Ginseng blade face
Or trickle irrigation is in the soil, or Paenibacillus polymyxa is mixed with other bacterium or organic fertilizer and is applied in farmland, more viscous classes
The concentration of bacillus bacteria suspension is 1 × 108~1 × 1010CFU/mL.The taste of American ginseng root maize ear rot sickle-like bacteria can efficiently be suppressed
It is raw.
Brief description of the drawings
Fig. 1 is bacterium colony photos of Paenibacillus polymyxa (Paenibacillus polymyxa) DN67 in LB culture mediums.
Fig. 2 is Paenibacillus polymyxa (Paenibacillus polymyxa) DN67 to American ginseng root maize ear rot sickle-like bacteria
Fungistatic effect.
Fig. 3 is that Paenibacillus polymyxa (Paenibacillus polymyxa) DN67 is based on 16S rRNA sequences
System development tree.
Preservation information：Paenibacillus polymyxa (Paenibacillus polymyxa) DN67, December 21 in 2017
Day registers preservation in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is：CGMCC
No.15112, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
Embodiment
The screening of embodiment 1, Paenibacillus polymyxa (Paenibacillus polymyxa) DN67
On July 2nd, 2016 gathers sample from Yantai City coastal waters deposit on marine-bottom surface, using method of dilution butteron on plate pair
Microorganism isolates and purifies, and weighs 10g mud samples and adds in the triangular flask equipped with 90mL sterile waters, 180r/min vibration 30min, gained
Suspension is diluted to 10-1、10-2、10-3、10-4、10-5Etc. different dilution factors, it is respectively coated on LB plating mediums, each dilution
Degree is repeated 2 times, and carton upside down culture 24h, picking single bacterium colony therein are cultivated in 30 DEG C, and plate streaking is purified 3 times, cultivated
Pure bacterial strain, is inoculated into the PDA culture medium of coating American ginseng root maize ear rot fungi Fusarinm solani respectively, and 2~3 are cultivated in 28 DEG C
My god, the bacterial strain that choosing has obvious inhibition zone is purified, preservation.
Above-mentioned strain access LB plating mediums are cultivated 2~3 days for 30 DEG C, are transferred with card punch into PDA culture medium, at the same time
An equal amount of American ginseng root maize ear rot fungi Fusarinm solani is placed in same tablet other end, carries out tablet face-off training
Support, fungistatic effect is shown in Fig. 2.The good bacterial strain of screening antagonistic effect identified, through morphology, physiological and biochemical analysis (being shown in Table 1) and
16S rRNA molecular biology identifications, are determined as Paenibacillus polymyxa (Paenibacillus polymyxa) DN67, are based on
The phylogenetic tree of 16S rRNA sequences is shown in Fig. 3.
Paenibacillus polymyxa DN67 forms milky bacterium colony on LB culture mediums, and translucent, bacterium colony is smooth, middle slightly convex
Rise, edge out-of-flatness, has provoked stickiness (see Fig. 1).
The physiological and biochemical property of 1 bacterial strain DN67 of table
The 16S rRNA sequences of Paenibacillus polymyxa DN67：
gggctttgggcggaggccgctaatacatgcagtcgagcggggttaggttagaagcttgcttctaacctaacctagcg
gcggacgggtgagtaacacgtaggcaacctgcccacaagacagggataactaccggaaacggtagctaatacccgat
acatccttttcctgcatgggagaaggaggaaaggcggagcaatctgtcacttgtggatgggcctgcggcgcattagc
tagttggtggggtaaaggcctaccaaggcgacgatgcgtagccgacctgagagggtgatcggccacactgggactga
gacacggcccagactcctacgggaggcagcagtagggaatcttccgcaatgggcgaaagcctgacggagcaacgccg
cgtgagtgatgaaggttttcggatcgtaaagctctgttgccagggaagaacgcttgatagagtaactgctcttgaag
tgacggtacctgagaagaaagccccggctaactacgtgccagcagccgcggtaatacgtagggggcaagcgttgtcc
ggaattattgggcgtaaagcgcgcgcaggcggctctttaagtctggtgtttaatcccgaggctcaacttcgggtcgc
actggaaactggggagcttgagtgcagaagaggagagtggaattccacgtgtagcggtgaaatgcgtagagatgtgg
aggaacaccagtggcgaaggcgactctctgggctgtaactgacgctgaggcgcgaaagcgtggggagcaaacaggat
tagataccctggtagtccacgccgtaaacgatgaatgctaggtgttaggggtttcgatacccttggtgccgaagtta
acacattaagcattccgcctggggagtacggtcgcaagactgaaactcaaaggaattgacggggacccgcacaagca
gtggagtatgtggtttaattcgaagcaacgcgaagaaccttaccaggtcttgacatccctctgaccggtctagagat
agatctttccttcgggacagaggagacaggtggtgcatggttgtcgtcagctcgtgtcgtgagatgttgggttaagt
cccgcaacgagcgcaacccttatgcttagttgccagcaggtcaagctgggcactctaagcagactgccggtgacaaa
ccggaggaaggtggggatgacgtcaaatcatcatgccccttatgacctgggctacacacgtactacaatggccggta
caacgggaagcgaaggagcgatgtggagccaatcctagaaaagccggtctcagttcggattgtaggctgcaactcgc
ctacatgaagtcggaattgctagtaatcgcggatcagcatgccgcggtgaatacgttcccgggtcttgtacacaccg
cccgtcacaccacgagagtttacaacacccgaagtcggtgaggtaaccgcaaggagccagccgctcgtatagatggg
atacgcagggc。
Embodiment 2, the fungistatic effect measure of Paenibacillus polymyxa (Paenibacillus polymyxa) DN67
The present embodiment provides a kind of biocontrol agent, the biocontrol agent is the zymotic fluid of Paenibacillus polymyxa DN67, its
Preparation method is as follows：
(1) the Paenibacillus polymyxa DN67 is inoculated in fluid nutrient medium and cultivated, to 20 times of dilutions of nutrient solution
OD600 is 0.3~0.8, obtains seed liquor；
The component and final concentration of the fluid nutrient medium:Glucose 1.6%, sodium chloride 0.25%, magnesium sulfate 0.05%, ferment
Mother's leaching powder 1%, ammonium chloride 0.7%, dipotassium hydrogen phosphate 0.1%, pH7.
(2) by seed liquor according to volume ratio 1~10%.Amount be inoculated in fermentation medium expand culture, expand culture
Temperature be 30 DEG C, the cell concentration into zymotic fluid reaches 1 × 109~1 × 1010CFU/mL, obtains the biocontrol agent.
The formula of the fermentation medium is：Glucose 1~2%, yeast extract 0.5~1.0%, dipotassium hydrogen phosphate 0.5
~1.0%, sodium chloride 0.2~0.5%, ammonium sulfate 0.5~1.0%, magnesium sulfate 0.03~0.05%, adjusting pH to 6.5~
7.5。
By biocontrol agent centrifuging and taking supernatant, 1 times, 10 times, 100 times is diluted respectively, takes 100 microlitres to be used for American ginseng root respectively
The antagonistic experiment of maize ear rot fungi Fusarinm solani, the results are shown in Figure 2, and fermented supernatant fluid, which dilutes 10 times, still has obvious suppression
Bacterium effect, antibacterial circle diameter 21mm.
Embodiment 3, Paenibacillus polymyxa (Paenibacillus polymyxa) DN67 sprout Fusarinm solani spore
The inhibitory action of hair
Final concentration of 10 are separately added into 2% sucrose solution, Paenibacillus polymyxa DN67 fermented supernatant fluids4A/mL
Fusarinm solani spore suspension, each treatment group respectively takes 10 μ L spore suspensions to be placed on glass slide, is placed in heat and moisture preserving training
28 DEG C of quiescent culture 10h of case are supported, observe the spore germination situation of each treatment group, calculate germination rate.Microscopy is it has been observed that more viscous class
Fusarinm solani spore in bacillus DN67 fermented supernatant fluids cannot form germ tube, and deformity expansion, rupture etc. occurs in spore
Phenomenon, germination rate 0, and the Fusarinm solani spore germination rate in 2% sucrose solution is 98%.
The effect of embodiment 4, field trial prevention American ginseng root maize ear rot
One piece of 3 years raw American Ginseng crop field is chosen, is divided into three pieces of ground of equal size, plot is CK blank controls, is used
Fusarinm solani spore liquid irrigating root, does not apply any microbial inoculum；Second piece of ground is treatment group 1, first uses Paenibacillus polymyxa
The fermentation liquid irrigating root of (Paenibacillus polymyxa) DN67, then uses Fusarinm solani spore liquid irrigating root again；3rd piece
Ground is treatment group 2, first with Fusarinm solani spore liquid irrigating root, then with Paenibacillus polymyxa (Paenibacillus
Polymyxa) the fermentation liquid irrigating root of DN67, compares the incidence of American ginseng root maize ear rot, it turns out that using Paenibacillus polymyxa
The plot American ginseng root maize ear rot incidence of (Paenibacillus polymyxa) DN67 zymotic fluids does not apply bacterium solution substantially less than
Control group.
The incidence of 2 different disposal group of table
Sequence table
<110>Ludong University
<120>A kind of Paenibacillus polymyxa and its application for being used to prevent American ginseng root maize ear rot
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1474
<212> DNA
<213>Paenibacillus polymyxa (Paenibacillus polymyxa)
<400> 1
gggctttggg cggaggccgc taatacatgc agtcgagcgg ggttaggtta gaagcttgct 60
tctaacctaa cctagcggcg gacgggtgag taacacgtag gcaacctgcc cacaagacag 120
ggataactac cggaaacggt agctaatacc cgatacatcc ttttcctgca tgggagaagg 180
aggaaaggcg gagcaatctg tcacttgtgg atgggcctgc ggcgcattag ctagttggtg 240
gggtaaaggc ctaccaaggc gacgatgcgt agccgacctg agagggtgat cggccacact 300
gggactgaga cacggcccag actcctacgg gaggcagcag tagggaatct tccgcaatgg 360
gcgaaagcct gacggagcaa cgccgcgtga gtgatgaagg ttttcggatc gtaaagctct 420
gttgccaggg aagaacgctt gatagagtaa ctgctcttga agtgacggta cctgagaaga 480
aagccccggc taactacgtg ccagcagccg cggtaatacg tagggggcaa gcgttgtccg 540
gaattattgg gcgtaaagcg cgcgcaggcg gctctttaag tctggtgttt aatcccgagg 600
ctcaacttcg ggtcgcactg gaaactgggg agcttgagtg cagaagagga gagtggaatt 660
ccacgtgtag cggtgaaatg cgtagagatg tggaggaaca ccagtggcga aggcgactct 720
ctgggctgta actgacgctg aggcgcgaaa gcgtggggag caaacaggat tagataccct 780
ggtagtccac gccgtaaacg atgaatgcta ggtgttaggg gtttcgatac ccttggtgcc 840
gaagttaaca cattaagcat tccgcctggg gagtacggtc gcaagactga aactcaaagg 900
aattgacggg gacccgcaca agcagtggag tatgtggttt aattcgaagc aacgcgaaga 960
accttaccag gtcttgacat ccctctgacc ggtctagaga tagatctttc cttcgggaca 1020
gaggagacag gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc 1080
cgcaacgagc gcaaccctta tgcttagttg ccagcaggtc aagctgggca ctctaagcag 1140
actgccggtg acaaaccgga ggaaggtggg gatgacgtca aatcatcatg ccccttatga 1200
cctgggctac acacgtacta caatggccgg tacaacggga agcgaaggag cgatgtggag 1260
ccaatcctag aaaagccggt ctcagttcgg attgtaggct gcaactcgcc tacatgaagt 1320
cggaattgct agtaatcgcg gatcagcatg ccgcggtgaa tacgttcccg ggtcttgtac 1380
acaccgcccg tcacaccacg agagtttaca acacccgaag tcggtgaggt aaccgcaagg 1440
agccagccgc tcgtatagat gggatacgca gggc 1474
Claims (10)
1. one plant of Paenibacillus polymyxa for being used for antagonism American ginseng root maize ear rot sickle-like bacteria, it is characterised in that it is more viscous class gemma
Bacillus DN67, has registered in China Committee for Culture Collection of Microorganisms's common micro-organisms center on December 21st, 2017 and has protected
Hide, deposit number is：CGMCC No.15112, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
2. one plant of Paenibacillus polymyxa for being used for antagonism American ginseng root maize ear rot sickle-like bacteria according to claim 1, its feature
The fermentation culture conditions for being the Paenibacillus polymyxa DN67 are 28~37 DEG C.
3. one plant of Paenibacillus polymyxa for being used for antagonism American ginseng root maize ear rot sickle-like bacteria according to claim 2, its feature
It is the component of fermentation medium and final concentration of：Glucose 1~2%, yeast extract 0.5~1.0%, dipotassium hydrogen phosphate 0.5
~1.0%, sodium chloride 0.2~0.5%, ammonium sulfate 0.5~1.0%, magnesium sulfate 0.03~0.05%, adjusting pH to 6.5~
7.5。
4. applications of the Paenibacillus polymyxa DN67 described in claim 1 in American ginseng root maize ear rot sickle-like bacteria medicine is prevented.
A kind of 5. biocontrol agent containing Paenibacillus polymyxa DN67 described in claim 1.
6. biocontrol agent according to claim 5, its spy is Paenibacillus polymyxa DN67 described in the biocontrol agent
Concentration be 1 × 108~1 × 1010CFU/mL or 1 × 108~1 × 1010CFU/g。
7. the preparation method of biocontrol agent described in claim 5, it is characterised in that it the step of include：
(1) the Paenibacillus polymyxa DN67 is inoculated in fluid nutrient medium and cultivated, to the dilution of 20 times of nutrient solution after OD600
For 0.3~0.8, seed liquor is obtained；
(2) seed liquor is inoculated in fermentation medium to expand and cultivated, the cell concentration into zymotic fluid reaches 1 × 109~1 ×
1010CFU/mL, obtains the biocontrol agent.
8. according to the method described in claim 7, it is characterized in that the inoculum concentration of the seed liquor is volume ratio 1~10%.
9. the according to the method described in claim 7, it is characterized in that component of the fermentation medium and final concentration of：Glucose
1~2%, yeast extract 0.5~1.0%, dipotassium hydrogen phosphate 0.5~1.0%, sodium chloride 0.2~0.5%, ammonium sulfate 0.5~
1.0%, magnesium sulfate 0.03~0.05%, adjusts pH to 6.5~7.5.
10. according to the method described in claim 7, it is characterized in that the temperature for expanding culture is 28~37 DEG C.
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| CN110804566A (en) * | 2019-11-22 | 2020-02-18 | 汉江师范学院 | Bacillus for preventing and treating root rot of paris polyphylla and preparation method and application thereof |
| CN111733120A (en) * | 2020-08-25 | 2020-10-02 | 鲁东大学 | Bacillus aryabhattai and application thereof in prevention and treatment of American ginseng diseases |
| CN112795507A (en) * | 2021-01-07 | 2021-05-14 | 河南省农业科学院植物保护研究所 | Paenibacillus polymyxa capable of preventing and treating root rot of salvia miltiorrhiza and application thereof |
| CN112812995A (en) * | 2021-01-07 | 2021-05-18 | 河南省农业科学院植物保护研究所 | Paenibacillus polymyxa capable of preventing and treating root rot of salvia miltiorrhiza and application thereof |
| CN114717155A (en) * | 2022-05-06 | 2022-07-08 | 兰州交通大学 | Paenibacillus polymyxa for preventing and treating root rot of codonopsis pilosula and application thereof |
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