CN103173386A - Bio-control strain G1 for preventing and controlling pepper phytophthora blights and applications thereof - Google Patents
Bio-control strain G1 for preventing and controlling pepper phytophthora blights and applications thereof Download PDFInfo
- Publication number
- CN103173386A CN103173386A CN201310079281XA CN201310079281A CN103173386A CN 103173386 A CN103173386 A CN 103173386A CN 201310079281X A CN201310079281X A CN 201310079281XA CN 201310079281 A CN201310079281 A CN 201310079281A CN 103173386 A CN103173386 A CN 103173386A
- Authority
- CN
- China
- Prior art keywords
- paenibacillus polymyxa
- microbial inoculum
- phytophthora capsici
- epidemic disease
- bacterial strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a bio-control strain G1 for preventing and controlling pepper phytophthora blights and applications thereof. The invention provides a paenibacillus polymyxa strain G1 the preservation No. of which is CGMCC No. 6762. The invention also provides a method for preparing a G1 bacteria agent, which comprises the following steps: fermenting the paenibacillus polymyxa strain G1, and collecting fermented products so as to obtain the G1 bacteria agent. Experiments prove that aiming at the current situation that no bio-control agent which achieves a good effect on preventing pepper phytophthora blight is provided at present; and the invention provides a single bacterium for preventing pepper phytophthora blights and a preparation thereof, which are specially applied to the prevention and control of pepper phytophthora blights. The bacteria agent has a good prevention and control effect on important soil-borne diseases, and the control effect on greenhouse pepper phytophthora blights reaches 71.18%.
Description
Technical field
The present invention relates to biological technical field, relate in particular to a kind of biocontrol strain G1 and application thereof that prevents and treats capsicum epidemic disease.
Background technology
Capsicum epidemic disease is a kind of oomycetes disease that is caused by phytophthora blight of pepper (Phytophthora capsici).Phytophthora is subordinate to oomycetes door, Peronosporales, pythiaceae, phytophthora.It is not with a kind of form that phytophthora is survived in soil, but is that the multiple change modes such as mycelium, sporocyst, chlamydospore and oospore are survived with different shape.This disease can be by rainwater and irrigation water, and agricultural equipment is propagated, and is the very strong soil-borne disease of a kind of hazardness.Along with China capsicum is planted in a large number, capsicum epidemic disease occurs at open country, protection ground and booth also big area, because this disease disease cycle is short, epidemic rate is fast, often causes pepper plant dead in flakes, brings serious financial loss to capsicum industry.General diseased plant rate is 20-30%, reaches more than 80% when serious, and this disease is one of principal element that affects China's capsicum industry Economic development.
Phytophthora capsici is a kind of heterothallic microorganism, and it has 2 kinds of main mating types: A1 type and A2 type.Groves etc. think, due to being widely used of sterilant in recent years, making phytophthora blight of pepper produce resistance, thereby induce the variation of its mating type, bring a lot of difficulties for the control of this disease.The bactericide speciallies such as phenylamide fungicide metalaxyl (metalaxyl) and Metalaxyl-M (mefenoxam) are widely used in preventing and treating this disease, be the series bactericidal agent that in present domestic and international widely used oomycetes disease control medicament, activity is high, usage quantity is large, and obtained good prevention effect.But because such medicament belongs to specificity site inhibitor, single to the pathogenic bacteria action site, life-time service very easily develops immunity to drugs, and causes prevention effect to descend.Methoxy acrylic bactericide is the another important systemic fungicide of control capsicum epidemic disease, because its mechanism of action is novel,, is widely used at present without cross resistance with the phenyl amide series bactericidal agent.But there are some researches show, some pathogenic bacteria target is higher to such medicament spontaneous mutation frequency that develops immunity to drugs, and pathogenic bacteria easily forms resistance colony under the high selective pressure of medicament.
In sum, what develop new, effective and safe microbial bactericide control capsicum epidemic disease is the demand that improves the capsicum production, and the while is the needs of capsicum industry economy, capsicum edible safety and agricultural sustainable development especially.
Summary of the invention
An object of the present invention is to provide a kind of Paenibacillus polymyxa Paenibacillus polymyxa bacterial strain G1.
Paenibacillus polymyxa Paenibacillus polymyxa bacterial strain G1 provided by the invention, its preserving number is CGMCC No.6762.
Another object of the present invention is to provide a kind of method for preparing control capsicum epidemic disease microbial inoculum.
Method provided by the invention comprises the steps: the Paenibacillus polymyxa Paenibacillus polymyxa bacterial strain G1 that ferments above-mentioned, obtains tunning, namely obtains microbial inoculum.
In aforesaid method, described obtain tunning after, also comprise the steps: to collect the bacterial sediment in described tunning, the more described bacterial sediment that suspends, obtain microbial inoculum.
Above-mentioned suspension suspension used is water.
In aforesaid method, the bacterial sediment in described collection tunning adopts centrifugal mode.Concrete centrifugal is the centrifugal 10min of 11100 * g.
In aforesaid method, the condition of described fermentation is as follows: 26-28 ℃, 180-200rpm shaking culture 24-36h; The condition of described fermentation is specific as follows: 26 ℃, 180rpm shaking culture 24h.
Microbial inoculum by the aforesaid method preparation; Viable bacteria total concn in described microbial inoculum is specially 1 * 10
7-1 * 10
10Cfu/mL, the viable bacteria total concn in microbial inoculum is 1 * 10 in an embodiment of the present invention
7Cfu/mL.
The 3rd purpose of the present invention is to provide a kind of control capsicum epidemic disease or antimicrobial product.
Product provided by the invention, its activeconstituents are above-mentioned Paenibacillus polymyxa Paenibacillus polymyxa bacterial strain G1 or above-mentioned microbial inoculum.
In the said products, described capsicum epidemic disease is for to be caused by Phytophthora capsici Phytophthora capsici; Above-mentioned Phytophthora capsici Phytophthora capsici is specially Phytophthora capsici Phytophthora capsici bacterial strain SD-33;
Described antibiotic at least a in anti-following 6 kinds of pathogenic bacterias: Valsa sordida bacterium Valsa sordida, ash arrhizus bacteria Botrytis cinerea, chestnut epidemic disease bacterium Cryphonectria parasitica, melon and fruit rotten mould Pythium aphanidermatum, dry thread Pyrenomycetes Rhizoctonia solani K ü hn and Phytophthora capsici Phytophthora capsici.
Above-mentioned Paenibacillus polymyxa Paenibacillus polymyxa bacterial strain G1 or above-mentioned microbial inoculum the control capsicum epidemic disease or antibiotic in application be also the scope of protection of the invention.
In above-mentioned application, described capsicum epidemic disease is for to be caused by Phytophthora capsici Phytophthora capsici; Above-mentioned Phytophthora capsici Phytophthora capsici is specially Phytophthora capsici Phytophthora capsici bacterial strain SD-33;
Described antibiotic at least a in anti-following 6 kinds of pathogenic bacterias: Valsa sordida bacterium Valsa sordida, ash arrhizus bacteria Botrytis cinerea, chestnut epidemic disease bacterium Cryphonectria parasitica, melon and fruit rotten mould Pythium aphanidermatum, dry thread Pyrenomycetes Rhizoctonia solani K ü hn and Phytophthora capsici Phytophthora capsici.
The application method of above-mentioned biocontrol fungicide control capsicum epidemic disease is:
Be 1 * 10 with the viable bacteria total concn when Hot Pepper in Greenhouse is grown seedlings
7Cfu/mL microbial inoculum seed soaking 1 hour, during sowing, then to water the viable bacteria total concn be 1 * 10
7Microbial inoculum; Grow to the 7-8 leaf during phase until pepper seedling, first (the viable bacteria total concn is 1 * 10 with the 20mLG1 microbial inoculum
7Cfu/mL) evenly be sprayed on whole basin Hot Pepper Seedling, after 10min, (the viable bacteria total concn is 1 * 10 with the 30mLG1 microbial inoculum with every basin again
7Cfu/mL) root irrigation.
Bacterium G1, be Paenibacillus polymyxa Paenibacillus polymyxa, on November 2nd, 2012, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (abbreviation CGMCC, the address is: the Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica), culture presevation number is: CGMCC No.6762.
Of the present invention experimental results show that, the present invention is directed to present capsicum epidemic disease does not have the biological prevention and control agent present situation of higher preventive effect, provide exploitation a kind of single bacterium Paenibacillus polymyxa Paenibacillus polymyxa G1 and microbial inoculum thereof of preventing and treating capsicum epidemic disease, be exclusively used in the control of capsicum epidemic disease, this bacterium or its microbial inoculum have higher prevention effect to this important soil-borne disease, and the prevention effect of Hot Pepper in Greenhouse epidemic disease reaches 71.18%.Because bacterial strain G1 of the present invention is the biocontrol strain that screening is developed for capsicum epidemic disease specially, thereby comparing with other broad spectrum biological disinfection agent at present aspect the control capsicum epidemic disease, prevention effect is remarkable.Owing to being biotechnological formulation, the series of problems that brings less than the use because of chemical pesticide fully, thereby be conducive to the nuisanceless production of crop, the peasant can or reduce the consumption of other control capsicum epidemic disease chemical pesticide, this not only can for the peasant reduces expenses, also be conducive to the outlet of capsicum product.This biological prevention and control agent increases production function in addition simultaneously, and can be the peasant increases income.
Description of drawings
Fig. 1 is that biocontrol bacteria is to the prevention effect of capsicum epidemic disease
Embodiment
The experimental technique that uses in following embodiment is ordinary method if no special instructions.
In following embodiment, material used, reagent etc., if no special instructions, all can obtain from commercial channels.
The separation of embodiment 1, biocontrol bacteria G1 and evaluation
1, strain separating and evaluation
(1) separation of bacterial strain
To rinse well with tap water from the capsicum plant of Shandong Agricultural University's scientific and technical innovation garden collection, and be divided into root, stem, leaf three parts, then use aseptic water washing 2 times, aseptic filter paper blots surface-moisture.Under aseptic condition, 75% alcohol immersion 1min, aseptic water washing 3 times changes 3% javelle water over to and soaks 5min, aseptic water washing 3 times, aseptic filter paper blots surface-moisture.After root, stem, the leaf of surface sterilization is placed in respectively the sterilization mortar and shreds, grind after adding appropriate sterilized water, redilution becomes 10
-1, 10
-2, 10
-3Three gradients get respectively 10
-2, 10
-3The lapping liquid of dilution gradient moves in the LA culture medium flat plates with each 200 μ L of last washing fluid, and coating is even, and last washing fluid is contrast CK, and every processing repeats 3 times, puts 28 ℃ of cultivations, observes day by day.Select in CK without bacterium colony, and the bacterial strain that has bacterium colony to grow in lapping liquid, according to feature picking list bacterium colonies such as colonial morphology, colors, repeatedly after the plate streaking purifying, the bacterial strain that purifying is good is numbered, and the inclined-plane saves backup.
LA substratum: peptone 10g, yeast powder 5g, NaCl5g, agar powder 17g, distilled water 1000mL, pH value 7.0.
(2) antagonism screening
Adopt dull and stereotyped face-off method to carry out the screening of antagonistic bacterium, screening with test plant pathogenic fungi Valsa sordida bacterium (Valsa sordida), ash arrhizus bacteria (Botrytis cinerea), chestnut epidemic disease bacterium (Cryphonectria parasitica), melon and fruit corruption mould (Pythium aphanidermatum), dry thread Pyrenomycetes (Rhizoctonia solani K ü hn) and Phytophthora capsici (Phytophthora capsici) bacterial strain SD-33(phytophthora blight of pepper antagonistic bacterium, the Shandong agricultural sciences, 2012,44 (9): 90 – 92; The public can obtain from Shandong Agricultural University) be inoculated in respectively the dull and stereotyped central authorities of PDA, the tested bacteria point is connected to apart from 2cm place, pathogenic bacteria left and right, every processing three wares, 28 ℃ of constant temperature are inverted and are cultivated after 4 days, measure its bacteriostasis rate (the results are shown in Table 1).
Bacteriostasis rate=(contrast flat-plate bacterial colony diameter-face-off flat-plate bacterial colony diameter)/contrast flat-plate bacterial colony diameter * 100%.
Selection has the endogenetic bacteria bacterial strain of stronger antagonistic activity to Phytophthora capsici, be numbered G1, preserves under-80 ℃ in 50% glycerine.
Table 1 is that bacterial strain G1 is to the bacteriostasis rate of various pathogenic bacteria
Annotate: with significant difference on different letter representations 0.05 level after column data.
2, the Morphology and physiology biochemical character of G1 detects
(1) bacterium colony and strain morphology feature
Bacterial strain G1 is faint yellow opaque colony on the LA substratum, surperficial thickness, and colony edge is irregular.Thalline is shaft-like, and the gramstaining reaction is positive, peritrichous, and the gemma end is given birth to, oval.
(2) physiological and biochemical property detects
Can utilize carbon source: dextrin, glycogen, L-arabinose, D-Fructose, D-semi-lactosi, alpha-D-glucose, α-D-lactose, maltose, PEARLITOL 25C, D-volemite, D-melibiose, Beta-methyl-D-Glucose glycosides, D-Psicose, D-raffinose, sucrose, D-trehalose, turanose, glycerol, α-D-glycerol sodium phosphate.
cannot utilize carbon source: alpha-cylodextrin, polysorbate40, tween 80, N-acetyl-glucosamine, D-R alcohol, L-fucose, gentiobiose, the m-fibres sugars, the L-rhamnosyl, the D-Sorbitol Powder, Xylitol, the pyruvic acid methyl ester, monomethyl succinate, acetic acid, the D-galactonolactone, the D-galacturonic acid, inosine, uridine, putrescine, alpha-hydroxybutyric acid, beta-hydroxy-butanoic acid, hydroxybutyric acid, p-hydroxyphenyl acetic acid, α-ketoglutaric acid, D, Pfansteihl, propionic acid, succsinic acid, succinamide, D-alanine, ALANINE, L-Ala-L-Gly, the L-asparagine, Pidolidone, glycyl-L-glutamic acid, the L-Pyroglutamic Acid, Serine, butyleneglycol, D-Glucose-6-phosphoric acid salt.
3, the Biolog microorganism automatic identifying system of G1 is analyzed
Bacterial isolates G1 streak inoculation to the LA plate culture medium, is cultivated 48h under 28 ℃, after growing single bacterium colony, deliver to State Key Laboratory for Microbial Technology of Shandong University and carry out the Biolog system identification.Authentication step is as follows: use the Biolog special culture media with purebred enlarged culturing.Prepare on request the bacteria suspension of certain turbidity (cell concn).With bacterial suspension inoculation to micropore identification plate (Microplate).Cultivate certain hour, the identification plate after cultivating is put into the readout instrument reading, software provides qualification result automatically.Start Biolog Microstation
TMWorkstation MicroLog
TM, enter MicroLog
TM3.4.20 system.Click SET UP, the initialize setting.Input sample number into spectrum (Sample Number) is selected identification plate type (Plate Type): GP2, selects Strain type (Strain type): GP-Rod SB, select incubation time (Incubation Time): 16-24h.Identification plate after cultivating is put on the carriage of readout instrument, taken off lid.Press " Read Next " key and begin reading, after readout instrument scanning identification plate, automatic spring, result is presented on computer screen.Start Biolog Microstation
TMWorkstation MicroLog
TM, enter MicroLog
TM3.4.20 system.Biolog Microbial Identification System qualification result shows that the Species ID of bacterial strain G1 is: Paenibacillus polymyxa, i.e. Paenibacillus polymyxa.
Identify through BIOLOG, bacterial strain G1 is the Gram-positive aerobic bacteria, based on the detected result of substrate utilization collection of illustrative plates (substrate utilizationprofiles) completely, bacterial strain G1 is Paenibacillus polymyxa Paenibacillus polymyxa (species ID).Wherein with reference culture matching degree SIM〉0.5; Can with other identification systems parameter PROB%=100% relatively.
System provides qualification result, and each result reflects with three indexs: i.e. possibility Probability(PROB), similarity Similarity(SIM) and distance of positions Distance(DIS).PROB can determine a bacterial classification near 100%, SIM 〉=0.5, and the two value of G1 is respectively 100% and 0.765, illustrates that qualification result is reliable.So far, tentative bacterial strain G1 belongs to Paenibacillus polymyxa Paenibacillus polymyxa.
4, the molecular biology identification of bacterial strain G1
Use TaKaRa MiniBEST Bacterial Genomic DNAKit Ver.2.0 test kit to extract the genomic dna of bacterial strain G1.Adopt the universal primer 27F(5-AGAGTTTGATCCTGGCTCAG-3 ' of bacterium) and 1492R (5'-TACGGTTAC CTTGTTACGATT-3'), take the genomic dna of bacterial strain G1 as template amplification 16SrDNA.Adopt 25 μ l reaction systems: 10 * PCR Buffer2.5 μ L; 25mM Mg
2+1.5 μ L; 2.5mM dNTP2 μ L; Primer 2 7F1 μ L; Primer 1492R1 μ L; 5U/ μ L Taq enzyme 0.2 μ L; Genomic templates DNA1 μ L; Add dd H
2O is to 25 μ L.The PCR response procedures is 94 ℃ of 5min, 94 ℃ of 30s, 50 ℃ of 1min, 72 ℃ of 1min, 30 circulations; 72 ℃ of 10min.After reaction finished, amplified production carried out electrophoresis, observations with 1% sepharose.Find 16S rDNA purpose band 1500bp, cut glue and reclaim.The purpose fragment is connected to carrier Peasy-T3, send China large gene sequencing.
Sequencing result is that the nucleotides sequence of the 16S rDNA of G1 is classified the sequence 1 in sequence table as.
Carry out BLAST homology sequence compare of analysis with 16S rDNA sequence known in the Genbank database, result is analyzed with MEG4.0 software.Final qualification result bacterial strain G1 is Paenibacillus polymyxa Paenibacillus polymyxa.
The G1 bacterial strain, Classification And Nomenclature is Paenibacillus polymyxa Paenibacillus polymyxa, on November 2nd, 2012, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (abbreviation CGMCC, the address is: the Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica), culture presevation number is: CGMCC No.6762.
Embodiment 2, the bacterium G1 application in the control capsicum epidemic disease
1, the preparation of G1 microbial inoculum
(1) preparation of seed liquor:
With the Paenibacillus polymyxa Paenibacillus polymyxa G1CGMCC No.6762 of embodiment 1 separating and preserving be seeded in contain on the LA substratum that concentration is 500 μ g/mL Vetstrep (Ster) drug resistance markers 28 ℃ cultivate activation after, immigration is equipped with in the triangular flask of 250mL of 100mL LB nutrient solution, 28 ℃, 180rpm shaking culture 24h obtains the G1 seed liquor.
LA substratum: peptone 10g, yeast powder 5g, NaCl5g, agar powder 17g, distilled water 1000mL, pH value 7.0; The LB substratum: do not add agar powder, other composition is identical with the LA substratum.
(2) preparation of microbial inoculum:
When the G1 seed liquor is cultured to the OD value 0.5 the time, the G1 seed liquor is added in the LB nutrient solution with the 1:100 volume ratio, 26 ℃, 180rpm shaking culture 24h; Then remove supernatant liquor with the centrifugal 10min of 11100 * g and collect bacterial sediment.
Bacterial sediment is suspended to dilute with sterilized water obtains the G1 microbial inoculum, and bacteria suspension concentration is 1 * 10
7-1 * 10
10Cfu/mL.
2, the application of bacterium G1 in the control capsicum epidemic disease
(1) preparation of potted plant soil
Adopt the ratio of matrix and land for growing field crops soil 2:1 to stir, stand-by after high pressure steam sterilization.
(2) capsicum potted plant growth
Capsicum (kind is especially big eggplant door pimento, by the glad Seed company production and sales of Chifeng exhibition) 28 ℃ of moisturizing vernalization of seed 3 days, after sprouting, (the viable bacteria total concn in the G1 microbial inoculum is 1 * 10 with the G1 microbial inoculum
7Cfu/mL) be seeded in nutrition pot after seed soaking 1h.(the viable bacteria total concn in the G1 microbial inoculum is 1 * 10 to every alms bowl pouring G1 microbial inoculum
7) 50mL, take water treatment as contrast.3 repetitions of every processing, every repetition 5 basins, 6 seeds of every basin.
When pepper seedling grows to the 7-8 leaf during phase, carry out the G1 microbial inoculum and strengthen the inoculation processing, specific as follows: first (the viable bacteria total concn is 1 * 10 with the 20mLG1 microbial inoculum
7Cfu/mL) evenly be sprayed on whole basin Hot Pepper Seedling, after 10min, (the viable bacteria total concn is 1 * 10 with the 30mLG1 microbial inoculum with every basin again
7Cfu/mL) root irrigation.The negative control group water treatment; The screening of the same concentrations of positive controls employing same procedure preparation and the Paenibacillus polymyxa strain X M(phytophthora blight of pepper antagonistic bacterium of same amount, Shandong agricultural sciences, 2012,44 (9): 90 – 92; The public can obtain from Shandong Agricultural University) bacteria suspension process.
(3) Phytophthora capsici inoculation
The G1 microbial inoculum is strengthened the rear second day of inoculation, is 1 * 10 with concentration
5The Phytophthora capsici of cfu/mL (Phytophthora capsici) bacterial strain SD-33(is documented in Phytophthora capsici (Phytophthora capsici) pectin methyl esterase Pcpme3 gene eucaryon expression and functional study, Scientia Agricultura Sinica 2009,42 (6): 1988-1993; The public can obtain from Shandong Agricultural University) inoculation of zoospore suspension root-pouring method, every strain pouring zoospore suspension 5mL.Before the inoculation prerequisite, the seedling alms bowl is filled with permeable, moisturizing 24h after inoculation is placed in indoorly, and water keeps moistening in good time.After inoculation, 7d investigation morbidity (capsicum epidemic disease) situation is also calculated prevention effect, according to the disease occurrence degree be divided into 6 grades (Lin Baiqing etc., the preliminary study [J] of the anti-epidemic disease authentication method of capsicum variety. China's Vegetable, 1994, (4): 21-25):
0 grade: anosis;
1 grade: the seedling basal part of stem slightly has blackening, but here plant health does not wither;
2 grades: the blackening of seedling basal part of stem reaches 1-2cm, and here the blade irrecoverability withers, and lower blade is even to be had and come off;
3 grades: more than the blackening of seedling basal part of stem reached 2cm, here blade obviously withered or obviously comes off;
4 grades: the seedling basal part of stem blackening contracting of hanging, all here fallen leaves or whole strain wither except vegetative point;
5 grades: plant is all withered.
Disease index=[∑ (sick level strain number * represent progression)/total strain number * superlative degree represents progression] * 100
Prevention effect %=(contrasts disease index-processing disease index)/contrast disease index * 100
Seedling stage, results from pot experiment test such as table 1 and shown in Figure 1, can find out, the capsicum of using the G1 microbial inoculum shows prevention effect preferably, reaches 71.18%.
The greenhouse prevention effect of table 2 biocontrol bacteria to capsicum epidemic disease
Annotate: the table intermediate value is three mean values that repeat.
Claims (10)
1. Paenibacillus polymyxa Paenibacillus polymyxa bacterial strain G1, its preserving number is CGMCC No.6762.
2. one kind prepares the method for preventing and treating the capsicum epidemic disease microbial inoculum, and the Paenibacillus polymyxa Paenibacillus polymyxa bacterial strain G1 claimed in claim 1 that comprises the steps: to ferment obtains tunning, namely obtains microbial inoculum.
3. method according to claim 2 is characterized in that: described obtain tunning after, also comprise the steps: to collect the bacterial sediment in described tunning, the more described bacterial sediment that suspends, obtain microbial inoculum.
4. according to claim 2 or 3 described methods, is characterized in that: the centrifugal mode of bacterial sediment employing in described collection tunning.
5. arbitrary described method according to claim 2-4, it is characterized in that: the condition of described fermentation is as follows: 26-28 ℃, 180-200rpm shaking culture 24-36h; The condition of described fermentation is specific as follows: 26 ℃, 180rpm shaking culture 24h.
6. the microbial inoculum that is prepared by arbitrary described method in claim 2-5; Viable bacteria total concn in described microbial inoculum is specially 1 * 10
7-1 * 10
10Cfu/mL.
7. prevent and treat capsicum epidemic disease or antimicrobial product for one kind, its activeconstituents is Paenibacillus polymyxa Paenibacillus polymyxa bacterial strain G1 claimed in claim 1 or microbial inoculum claimed in claim 6.
8. product according to claim 7, it is characterized in that: described capsicum epidemic disease is for to be caused by Phytophthora capsici Phytophthora capsici;
Described antibiotic at least a in anti-following 6 kinds of pathogenic bacterias: Valsa sordida bacterium Valsa sordida, ash arrhizus bacteria Botrytis cinerea, chestnut epidemic disease bacterium Cryphonectria parasitica, melon and fruit rotten mould Pythium aphanidermatum, dry thread Pyrenomycetes Rhizoctonia solani K ü hn and Phytophthora capsici Phytophthora capsici.
Paenibacillus polymyxa Paenibacillus polymyxa bacterial strain G1 claimed in claim 1 or microbial inoculum claimed in claim 6 the control capsicum epidemic disease or antibiotic in application.
10. application according to claim 9 is characterized in that: described capsicum epidemic disease is for to be caused by Phytophthora capsici Phytophthora capsici;
Described antibiotic at least a in anti-following 6 kinds of pathogenic bacterias: Valsa sordida bacterium Valsa sordida, ash arrhizus bacteria Botrytis cinerea, chestnut epidemic disease bacterium Cryphonectria parasitica, melon and fruit rotten mould Pythium aphanidermatum, dry thread Pyrenomycetes Rhizoctonia solani K ü hn and Phytophthora capsici Phytophthora capsici.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310079281.XA CN103173386B (en) | 2013-03-12 | 2013-03-12 | Bio-control strain G1 for preventing and controlling pepper phytophthora blights and applications thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310079281.XA CN103173386B (en) | 2013-03-12 | 2013-03-12 | Bio-control strain G1 for preventing and controlling pepper phytophthora blights and applications thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103173386A true CN103173386A (en) | 2013-06-26 |
| CN103173386B CN103173386B (en) | 2015-01-28 |
Family
ID=48633580
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310079281.XA Expired - Fee Related CN103173386B (en) | 2013-03-12 | 2013-03-12 | Bio-control strain G1 for preventing and controlling pepper phytophthora blights and applications thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103173386B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105039383A (en) * | 2015-08-18 | 2015-11-11 | 北京市农林科学院 | Construction method and application of recombinant Streptomyces lydicus for inhibiting plant pathogenic fungi |
| CN105557347A (en) * | 2014-10-13 | 2016-05-11 | 江西省农业科学院植物保护研究所 | Gray mold resistance identified seedling stage inoculation method for capsicum |
| CN108559716A (en) * | 2017-10-24 | 2018-09-21 | 安徽省农业科学院植物保护与农产品质量安全研究所 | One plant of efficient biocontrol bacterial strain for preventing capsicum epidemic disease and its application |
| CN108893424A (en) * | 2018-07-06 | 2018-11-27 | 四川农业大学 | A kind of bacillus megaterium BM22 and its microcapsule formulation and application |
| CN109077067A (en) * | 2018-08-16 | 2018-12-25 | 南京农业大学 | A kind of biocontrol microorganisms and its application in terms of crops gray mold prevention and treatment |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101215543A (en) * | 2008-01-18 | 2008-07-09 | 新疆农业科学院微生物应用研究所 | Strain for preventing and curing muskmelon bacteroidal spot disease and application thereof in field disease prevention |
| CN101519639A (en) * | 2008-02-27 | 2009-09-02 | 中国科学院生态环境研究中心 | Paenibacillus polymyxa for preventing and treating plant fungal diseases and production method thereof |
| CN102250815A (en) * | 2011-07-21 | 2011-11-23 | 中国热带农业科学院香料饮料研究所 | Endogenous paenibacillus polymyxa |
| CN102433285A (en) * | 2011-12-23 | 2012-05-02 | 仲恺农业工程学院 | Bacterium paenibacillus polymyma SHL-1 and application thereof in preventing and controlling stalk rot of cymbidium sinense |
| CN102952762A (en) * | 2011-08-26 | 2013-03-06 | 青岛群恒生物科技有限公司 | Paenibacillus polymyxa for controlling plant fungal diseases and production method thereof |
-
2013
- 2013-03-12 CN CN201310079281.XA patent/CN103173386B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101215543A (en) * | 2008-01-18 | 2008-07-09 | 新疆农业科学院微生物应用研究所 | Strain for preventing and curing muskmelon bacteroidal spot disease and application thereof in field disease prevention |
| CN101519639A (en) * | 2008-02-27 | 2009-09-02 | 中国科学院生态环境研究中心 | Paenibacillus polymyxa for preventing and treating plant fungal diseases and production method thereof |
| CN102250815A (en) * | 2011-07-21 | 2011-11-23 | 中国热带农业科学院香料饮料研究所 | Endogenous paenibacillus polymyxa |
| CN102952762A (en) * | 2011-08-26 | 2013-03-06 | 青岛群恒生物科技有限公司 | Paenibacillus polymyxa for controlling plant fungal diseases and production method thereof |
| CN102433285A (en) * | 2011-12-23 | 2012-05-02 | 仲恺农业工程学院 | Bacterium paenibacillus polymyma SHL-1 and application thereof in preventing and controlling stalk rot of cymbidium sinense |
Non-Patent Citations (3)
| Title |
|---|
| 万学瑞: "辣椒疫病生物防治的研究", 《中国优秀硕士学位论文全文数据库》, no. 9, 15 September 2006 (2006-09-15) * |
| 梅新兰 等: "辣椒疫病结抗菌株筛选、鉴定及其防效", 《应用生态学报》, vol. 21, no. 10, 31 October 2010 (2010-10-31), pages 2652 - 2658 * |
| 袁树忠: "辣椒疫霉拮顽菌的筛选鉴定、生防机制及与杀菌剂的协同作用", 《中国博士学位论文全文数据库 农业科学辑》, no. 6, 15 June 2012 (2012-06-15) * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557347A (en) * | 2014-10-13 | 2016-05-11 | 江西省农业科学院植物保护研究所 | Gray mold resistance identified seedling stage inoculation method for capsicum |
| CN105557347B (en) * | 2014-10-13 | 2018-11-20 | 江西省农业科学院植物保护研究所 | The Seedling Inoculation method of Botrytis cinerea Disease Resistance Identification |
| CN105039383A (en) * | 2015-08-18 | 2015-11-11 | 北京市农林科学院 | Construction method and application of recombinant Streptomyces lydicus for inhibiting plant pathogenic fungi |
| CN105039383B (en) * | 2015-08-18 | 2018-09-25 | 北京市农林科学院 | Inhibit construction method and the application of the recombination streptomyces lydicus of plant pathogenic fungi |
| CN108559716A (en) * | 2017-10-24 | 2018-09-21 | 安徽省农业科学院植物保护与农产品质量安全研究所 | One plant of efficient biocontrol bacterial strain for preventing capsicum epidemic disease and its application |
| CN108559716B (en) * | 2017-10-24 | 2023-04-14 | 安徽省农业科学院植物保护与农产品质量安全研究所 | High-efficiency biocontrol strain for preventing and treating pepper phytophthora blight and application thereof |
| CN108893424A (en) * | 2018-07-06 | 2018-11-27 | 四川农业大学 | A kind of bacillus megaterium BM22 and its microcapsule formulation and application |
| CN109077067A (en) * | 2018-08-16 | 2018-12-25 | 南京农业大学 | A kind of biocontrol microorganisms and its application in terms of crops gray mold prevention and treatment |
| CN109077067B (en) * | 2018-08-16 | 2021-04-30 | 南京农业大学 | Biocontrol bacterium and application thereof in prevention and control of gray mold of crops |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103173386B (en) | 2015-01-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2021185021A1 (en) | Microbial agent yf beneficial to corn growth in saline-alkali soil and application thereof | |
| CN102433282B (en) | Bacillus subtilis NB12, as well as culture method and application thereof | |
| CN101525587B (en) | Streptomyces strain and application thereof | |
| CN105886428A (en) | Streptomyces albidoflavus and applications thereof in microbial fertilizers | |
| CN110066756B (en) | Paenibacillus kribbensis and preparation and application thereof | |
| CN103224904B (en) | Rhodopseudomonas strain, biocontrol microbial inoculum and biocontrol fermentation liquid, and corresponding preparation methods and application thereof in controlling phytophthora blight of pepper | |
| CN106148232B (en) | The bacterium bacterial strain and its application of one plant of antagonism plant pathogenetic bacteria | |
| CN105543132A (en) | Bacillus methylotrophicus YB-F7 and application thereof in preventing plant diseases | |
| CN104877926B (en) | The bacterial strain of one plant of antagonism smoke tree wilt and its application | |
| CN105670964B (en) | Bacillus atrophaeus BsR05 strain and application thereof | |
| CN103173386B (en) | Bio-control strain G1 for preventing and controlling pepper phytophthora blights and applications thereof | |
| CN102787084B (en) | The Methylotrophic Bacillus strain 4-L-16 of a kind of preventing and controlling banana fusarium wilt and application thereof | |
| CN110358710A (en) | One plant of bacillus laterosporus and preparing the application in disease-resistant saline-alkali tolerant functional microorganism preparation | |
| CN101215543A (en) | Strain for preventing and curing muskmelon bacteroidal spot disease and application thereof in field disease prevention | |
| CN107502565B (en) | One plant of fritillary bulb rhizosphere Paenibacillus polymyxa and its application | |
| CN108641989A (en) | One plant of Methylotrophic bacillus and its application | |
| CN101942403B (en) | Bacillus pumilus as well as culture method and application thereof | |
| CN105925498B (en) | One pseudomonas category bacterial strain ST4 and its application in prevention and treatment sugarcane whip smut | |
| CN107699526A (en) | One plant of actinomycetes strain for preventing and treating gray mold and its application | |
| CN104630072A (en) | Trichoderma atroviride Ta-9 strain and application thereof in prevention and control of rice diseases | |
| CN110317747A (en) | A kind of bacillus amyloliquefaciens JT68 and its application in prevention and treatment tea anthracnose | |
| CN105176893B (en) | A kind of bacillus amyloliquefaciens and its application for preventing crop damping-off | |
| CN101519641B (en) | Method for producing mixed microorganism inoculum | |
| CN105176885A (en) | Bacillus subtilis and application of bacillus subtilis in degrading organophosphorus pesticide | |
| CN102018000B (en) | Application of BZ6-1 bacterial strain in preparing drugs for treating plant peanut bacterial wilt |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150128 Termination date: 20180312 |