CN107898732A - It is a kind of that there is whitening, the composition and its application, skin care item of light spot effect - Google Patents
It is a kind of that there is whitening, the composition and its application, skin care item of light spot effect Download PDFInfo
- Publication number
- CN107898732A CN107898732A CN201711476629.3A CN201711476629A CN107898732A CN 107898732 A CN107898732 A CN 107898732A CN 201711476629 A CN201711476629 A CN 201711476629A CN 107898732 A CN107898732 A CN 107898732A
- Authority
- CN
- China
- Prior art keywords
- composition
- extract
- whitening
- light spot
- spot effect
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention provides a kind of with whitening, the composition and its application, skin care item of light spot effect.Composition provided by the invention includes hydrolysis conchiolin, Bulbus Lilii extract, Caulis Bambusae In Taeniam extract, Ganodenna Lucidum P.E and ginger-root extract.The present invention selects Bulbus Lilii extract, Caulis Bambusae In Taeniam extract, four kinds of Chinese medical extracts of Ganodenna Lucidum P.E and ginger-root extract and hydrolysis conchiolin to be compounded; each component reasonable formula, coordinated, resulting composition can effectively protect the skin from damage caused by UVB irradiations with significantly whitening, light spot effect.
Description
Technical field
It is more particularly to a kind of that there is whitening, the composition of light spot effect and its application, shield the present invention relates to cosmetic field
Skin product.
Background technology
Recently as environmental pollution, rhythm of life is accelerated, night life is enriched, and more and more people are chronically at sub- strong
Health state, so as to cause phenomena such as dim heavy, color spot of skin increases, aging is accelerated increasingly to be aggravated.Everybody has a love of beauty, smooth
The skin of whitening is the target that everybody pursues, particularly female friend, and with the raising of living standard, people progressively pursue
Quality of the life, pursues crude.
At present, the skin care item of in the market are mainly used as active ingredient using various natural plant extracts.Wherein, Whitening, spot
Though skin care item species is various, effect is uneven, and mostly not notable to the removal effect of melanin, Whitening, spot effect is not
It is preferable.
The content of the invention
Present invention solves the technical problem that it is to provide a kind of with whitening, the composition of light spot effect and its application, shield
Skin product, said composition can effectively inhibit the formation of melanin, have significantly whitening, light spot effect.
The invention discloses it is a kind of with whitening, light spot effect composition, in parts by weight, including：
Preferably, in parts by weight, the composition includes：
In some specific embodiments provided by the invention, the composition includes：
0.01 part of conchiolin of hydrolysis, 3 parts of Bulbus Lilii extract, 5 parts of Caulis Bambusae In Taeniam extract, 3 parts of Ganodenna Lucidum P.E, race carry
Take 1.5 parts of thing.
In some specific embodiments provided by the invention, the composition includes：
0.05 part of conchiolin of hydrolysis, 3 parts of Bulbus Lilii extract, 3 parts of Caulis Bambusae In Taeniam extract, 5 parts of Ganodenna Lucidum P.E, race carry
Take 2 parts of thing.
In some specific embodiments provided by the invention, the composition includes：
Hydrolyze 0.1 part of conchiolin, 0.5 part of Bulbus Lilii extract, 1 part of Caulis Bambusae In Taeniam extract, 1 part of Ganodenna Lucidum P.E, race
0.2 part of extract.
Preferably, the hydrolysis conchiolin preparation method is：
Step 1:Crushing pearl is taken, adds water to stir to obtain pearl slurry, adds lactic acid, react 1.5h~3h in 95~100 DEG C,
Filtering, collects filtrate and filter residue respectively；
Step 2：Take filter residue to add water, adjust pH value to 6.0~8.0, add neutral proteinase, 39~41 DEG C of enzyme digestion reactions
2.5~4h, filtering, collects filtrate；
Step 3：The filtrate of combining step 1 and step 2, mixes, and centrifugation, freeze, and obtains hydrolysis conchiolin.
Wherein, in step 2, preferably by pH value to 7.0, the number of the enzyme digestion reaction is preferably 2 times, the temperature of enzyme digestion reaction
Degree is preferably 40 DEG C, and enzymolysis time is preferably 3h.
Preferably, in composition of the present invention, the preparation method of the Bulbus Lilii extract is：Using lily petal as
Explant, lily callus is obtained through Fiber differentiation, successively through secondary culture, Shaking culture, is scattered in after cleaning in glycerine,
Obtain Bulbus Lilii extract.Wherein, lily petal preferably uses the petal of lily, it is preferred to use N6 culture medium Fiber differentiations obtain hundred
Close callus.
In some specific embodiments provided by the invention, the preparation method of the Bulbus Lilii extract is：
The lily petal after sterilizing is cut, as explant, explant is placed in Fiber differentiation in N6 solid mediums, is obtained
To lily callus；The preferable cell mass secondary culture of body is chosen from callus, so as to obtain loose particles shape
Cell mass；It is filtered, the cell mass of individual cells or peanut is filtered out as competent cell, moves to the shaking flask equipped with nutrient solution
Middle carry out Shaking culture, obtains free cell.Then free cell is put on gauze, is rinsed with clear water, remove nutrient solution
In impurity, be finally scattered in glycerine, obtain Bulbus Lilii extract.
In the present invention in the composition, the Caulis Bambusae In Taeniam extract and Ganodenna Lucidum P.E are water extract, are this area skill
Known to art personnel, it can be commercially available by commercial sources or be raised by disclosed method water in the prior art, it is advised
Lattice meet country or professional standard.
Preferably, the Caulis Bambusae In Taeniam extract, Ganodenna Lucidum P.E are raised by dictyophora phalloidea, ganoderma lucidum through water respectively.It is highly preferred that
The Caulis Bambusae In Taeniam extract, Ganodenna Lucidum P.E are extracted 2 times by dictyophora phalloidea, ganoderma lucidum, race with the water of 20~30 times of amounts respectively, and each 1h~
5h, filters, is concentrated to give.
In some specific embodiments provided by the invention, the preparation method of the Caulis Bambusae In Taeniam extract is：
Dictyophora phalloidea is taken, is extracted 2 times with the water of 20~30 times of amounts, first time 2h, second of 1h, merges extracting solution twice, filter,
Constant weight is concentrated into, obtains Caulis Bambusae In Taeniam extract.
The preparation method of the Ganodenna Lucidum P.E is：
Ganoderma lucidum is taken, is extracted 2 times with the water of 20~30 times of amounts, first time 5h, second of 1h, merges extracting solution twice, filter,
Constant weight is concentrated into, obtains Ganodenna Lucidum P.E.
In the present invention in the composition, the ginger-root extract is alcohol extract, is known to the skilled person,
It can be commercially available by commercial sources or extract to obtain by disclosed method in the prior art, its specification meets country or row
Industry standard.
Preferably, the ginger-root extract is obtained by 95% EtOH Sonicate mechanical shaking extraction of race.
In some specific embodiments provided by the invention, the preparation method of the ginger-root extract is specially：
Race is taken, 35~42 DEG C of heat treatment 1h, crush, 95% EtOH Sonicate mechanical shaking extraction 3 times, each 1h, merging carries
Liquid is taken, is filtered, it is 18mg/ml~30mg/ml that filtrate, which is concentrated into mass concentration, obtains ginger-root extract.
Wherein, the temperature of heat treatment is preferably 40 DEG C, and it is 20mg/ml preferably to concentrate the filtrate to mass concentration.
Present invention also offers above-mentioned composition prepare with whitening, light spot effect cosmetics in application.
Present invention also offers a kind of skin care item with whitening, light spot effect, by can on above-mentioned composition and cosmetics
The auxiliary material of receiving is made.
The present invention's has whitening, the composition of light spot effect, in parts by weight, including：Hydrolyze conchiolin 0.01
~1 part；0.01~3 part of Flos Lilii viriduli extract；0.01~5 part of Caulis Bambusae In Taeniam extract；0.01~5 part of Ganodenna Lucidum P.E；Race extracts
0.01~2 part of thing.The present invention selects four kinds of Bulbus Lilii extract, Caulis Bambusae In Taeniam extract, Ganodenna Lucidum P.E and ginger-root extract Chinese medicines to carry
Thing is taken to be compounded with hydrolysis conchiolin, each component reasonable formula, coordinated, resulting composition can significantly reduce
The expression quantity of LEF-1 and the activity of tyrosinase are so as to reduce the synthesis of melanin；And improve Laminin332 and collagen I
Expression quantity to improve the barrier ability of skin.Damage caused by UVB irradiations is protected the skin from, and naturally-aged can be recovered
Proteasome subunit activity in cell, slows down aging, has significantly whitening, light spot effect.
Embodiment
The invention discloses a kind of composition and its application, skin care item with whitening, light spot effect, people in the art
Member can use for reference present disclosure, be suitably modified technological parameter realization.In particular, all similar substitutions and modifications
Apparent to those skilled in the art, they are considered as being included in the present invention.The method of the present invention and application
Be described by preferred embodiment, related personnel substantially can not depart from present invention, in spirit and scope it is right
Method described herein and application are modified or suitably change with combining, to realize and using the technology of the present invention.
With reference to embodiment, the present invention is further explained：
For a further understanding of the present invention, with reference to embodiment to provided by the invention with whitening, light spot effect
Composition and its application, skin care item illustrate, and protection scope of the present invention is not limited by the following examples.
The 3D skin models used in embodiment are purchased from French Lab Skin Creations；Proteasome test agent
Box (is purchased from Sigma companies).
The preparation of 1 composition of embodiment
(1) take pearl to clean, dry, crushed 100 mesh sieves, weighing 200g pearl powders, a small amount of deionized water being added, in water
Pearl slurry is stirred into bath, when temperature is increased to 90 DEG C, 430ml lactic acid is added, boils, 95~100 DEG C of reaction 2h, are constantly stirred
Mix, after reaction, filtering, filtrate is individually stored, and filter residue adds appropriate amount of deionized water, with Ca (OH)2PH value is adjusted to 7.0, is added
Enter 0.2g neutral proteinases, react 3h at 40 DEG C；Then 0.2g neutral proteinases are added, 3h, reaction knot are reacted at 40 DEG C
Shu Hou, filtering, merges front and rear filtrate twice, mixes, and ultracentrifuge surpasses from rear, freezes, up to hydrolysis conchiolin.
(2) lily is taken, sterilization, cuts the lily petal after sterilizing, as explant, explant is placed in N6 solids
Fiber differentiation in culture medium, obtains lily callus；The preferable cell mass secondary culture of body is chosen from callus, is obtained
Obtain the cell mass of loose particles shape；It is filtered, the cell mass of individual cells or peanut is filtered out as competent cell, moves to dress
Have and Shaking culture is carried out in the shaking flask of nutrient solution, obtain free cell.Then free cell is put on gauze, is rushed with clear water
Wash, remove the impurity in nutrient solution, be finally scattered in glycerine, obtain Bulbus Lilii extract.
(3) dictyophora phalloidea is taken, is extracted 2 times with the water of 20 times of amounts, first time 2h, second of 1h, merges extracting solution twice, filter,
Constant weight is concentrated into, obtains Caulis Bambusae In Taeniam extract.
(4) ganoderma lucidum is taken, is extracted 2 times with the water of 30 times of amounts, first time 5h, second of 1h, merges extracting solution twice, filter,
Constant weight is concentrated into, obtains Ganodenna Lucidum P.E.
(5) race is taken, 40 DEG C of heat treatment 1h, crush, weigh the sample 2g after crushing, add 500ml95% ethanol, often
The lower sonic oscillation of temperature extracts 3 times, each 1h, merges extracting solution, filtering, filtrate is concentrated into 40 DEG C of mass concentrations in rotary evaporator
For 20mg/ml, ginger-root extract is obtained.
Take above-mentioned 0.01 part of hydrolysis conchiolin, 3 parts of Bulbus Lilii extract, 5 parts of Caulis Bambusae In Taeniam extract, 3 parts of Ganodenna Lucidum P.E,
1.5 parts of mixing of ginger-root extract, obtain having whitening, the composition of light spot effect.
The preparation of 2 composition of embodiment
(1) take pearl to clean, dry, crushed 100 mesh sieves, weighing 200g pearl powders, a small amount of deionized water being added, in water
Pearl slurry is stirred into bath, when temperature is increased to 90 DEG C, 430ml lactic acid is added, boils, 95~100 DEG C of reaction 2.5h, constantly
Stirring, after reaction, filtering, filtrate are individually stored, and filter residue adds appropriate amount of deionized water, with Ca (OH)2PH value is adjusted to 7.0,
0.2g neutral proteinases are added, react 4h at 39 DEG C；0.2g neutral proteinases are added, 2h are reacted at 41 DEG C, reaction terminates
Afterwards, filter, merge front and rear filtrate twice, mix, ultracentrifuge surpasses from rear, freezes, up to hydrolysis conchiolin.
(2) lily is taken, sterilization, the lily petal then cut after sterilizing is explant, and explant is placed in N6 consolidates
Fiber differentiation in body culture medium, obtains lily callus；From callus choose the preferable cell mass of body, form compared with
Good cell mass secondary culture, so as to obtain the cell mass of loose particles shape；Free plant cell is filtered, is screened single thin
The cell mass of born of the same parents or peanut moves to and Shaking culture is carried out in the shaking flask equipped with nutrient solution, obtain free as competent cell
Cell.Then free cell is put on gauze, is rinsed with clear water, removed the impurity in nutrient solution, be finally scattered in glycerine,
Obtain Bulbus Lilii extract.
(3) dictyophora phalloidea is taken, is extracted 2 times with the water of 20 times of amounts, first time 2h, second of 1h, merges extracting solution twice, filter,
Constant weight is concentrated into, obtains Caulis Bambusae In Taeniam extract.
(4) ganoderma lucidum is taken, is extracted 2 times with the water of 30 times of amounts, first time 5h, second of 1h, merges extracting solution twice, filter,
Constant weight is concentrated into, obtains Ganodenna Lucidum P.E.
(5) race is taken, 40 DEG C of heat treatment 1h, crush, weigh the sample 2g after crushing, add 500ml95% ethanol, often
The lower sonic oscillation of temperature extracts 3 times, each 1h, merges extracting solution, filtering, filtrate is concentrated into 40 DEG C of mass concentrations in rotary evaporator
For 20mg/ml, ginger-root extract is obtained.
Take above-mentioned 0.05 part of hydrolysis conchiolin, 2 parts of Bulbus Lilii extract, 3 parts of Caulis Bambusae In Taeniam extract, 5 parts of Ganodenna Lucidum P.E,
2 parts of mixing of ginger-root extract, obtain having whitening, the composition of light spot effect.
The preparation of 3 composition of embodiment
Hydrolyze 0.1 part of conchiolin, 0.5 part of Bulbus Lilii extract, 1 part of Caulis Bambusae In Taeniam extract, 1 part of Ganodenna Lucidum P.E, race
The composition with whitening, light spot effect is prepared with embodiment 1 in 0.2 part of extract, preparation method.
The preparation of 4 composition of embodiment
0.05 part of conchiolin, 1 part of Bulbus Lilii extract, 2 parts of Caulis Bambusae In Taeniam extract, 2 parts of Ganodenna Lucidum P.E are hydrolyzed, race carries
1 part of thing is taken, the composition with whitening, light spot effect is prepared with embodiment 1 in preparation method.
The preparation of 5 composition of embodiment
1 part of conchiolin, 0.01 part of Bulbus Lilii extract, 0.01 part of Caulis Bambusae In Taeniam extract, 0.01 part of Ganodenna Lucidum P.E are hydrolyzed,
The composition with whitening, light spot effect is prepared with embodiment 1 in 0.01 part of ginger-root extract, preparation method.
The effect of 6 present composition of embodiment testing experiment
The formula of 1~5 composition of comparative example is shown in Table 1, and preparation method is the same as embodiment 1.Wherein, every test uses 3D skins
Skin model.The dosage of each group UVB radiation treatments is 100mJ/cm2, one time a day, continuous 1d.
1 experiment packet of table
Experimental project is：LEF-1 expression (Whitening, spot effect), tyrosinase and epidermis melanin (Whitening, spot work(
Effect), collagen I (senile-resistant efficacy), Laminin332 (moisture-keeping efficacy).
(1) skin barrier aptitude tests
TCF/LEF-1 paths adjust the barrier function of skin by regulating and controlling epidermal differentiation and fat metabolism.The present embodiment
Enhancing skin barrier aptitude tests are carried out to the present composition using 3D skin models.
Handled according to the packet of table 1, the effective ingredient consumption for the composition that each treatment group adds is 1% respective combination
Thing, is then marked LEF-1 using immunohistochemical method, and the expression of LEF-1 is as shown in table 2.
(2) tyrosinase activity is tested
Tyrosinase is the rate-limiting enzyme in melanin metabolism, is most important enzyme in melanin synthesis process.Use enzymatic
The activity of dynamics hair test tyrosinase.Handled according to the packet of table 1, the active ingredient for the composition that each treatment group adds
Dosage is 1% correspondent composition, and test method is as follows：
After the completion of cell incubation, remove culture medium and put after rinsing human melanocytes using phosphate buffered saline solution (PBS)
Enter in 1% Triton X-100 (triton x-100) solution (Sigma, France), and be incubated 10 minutes.When at 10nM
Without Ca2+And Mg2+PBS solution add L-3,4 dihydroxyphenylalanine amine (Sigma, France) start enzymatic reaction.
After being incubated one hour under 37 DEG C of dark surrounds, by using extinction of the spectrophotometer measurement at 475nm
Degree assessment tyrosinase activity.
(3) skin moisture-keeping aptitude tests
Laminin332 is distributed mainly on hyaline layer, the Integrin α6 β4 on cross-film hemidesmosome be laminin 5 by
Body.Itself and VII collagen type are bonded anchoring filament, and the papillaris pars of corium is inserted directly into through hyaline layer, fine and close lower floor, fixed
Skin corium；But also form covalent complex, the compound in basilar memebrane with laminin 6 or laminin 7
(Laminin 5~6/7) can directly be reacted through nestin with type Ⅳ collagen albumen.Therefore the presence of laminin 332 is steady
Determine the principal element of epidermal-dermal connective stability.The present embodiment strengthens the present composition using 3D skin models
Skin barrier aptitude tests.
Handled according to the packet of table 1, the effective ingredient consumption for the composition that each treatment group adds is 1% respective combination
Then thing is marked Laminin332 using immunohistochemical method, the expression feelings of Laminin332 after observation processing
Condition, the results are shown in Table 2.
(4) test of collagen I
By detecting the expression quantity of collagen I to observe composition anti-aging effects collagen I be outside Skin Cell
The main constituents of matrix, and the mark of skin senescence.The present embodiment using 3D skin models to the present composition into
Row test.
Handled according to the packet of table 1, identical (1% composition of effective ingredient consumption for the composition that each treatment group adds
Active ingredient), then collagen is marked using immunohistochemical method, the expression such as table 2 of collagen I
It is shown.
Table 2LEF-1, Laminin332, the expression quantity of collagen I and tyrosinase activity analysis
The results show that compared with UVB irradiation groups, 1 composition+UVB irradiations group of embodiment, 2 composition+UVB of embodiment shine
Penetrate group, 3 composition+UVB irradiations group of embodiment, embodiment 4 composition+UVB, embodiment 5+UVB the irradiation each table of composition group
Chrotoplast length unit LEF-1 positive cell numbers (P<0.01) and tyrosinase activity significantly reduces (P<0.001)；
Laminin332 and collagen I positive cell numbers (P<0.01) dramatically increase.
Compared with 1~5 compositions-treated group of comparative example, each epidermal cell length unit after 1 compositions-treated of embodiment
LEF-1 positive cell numbers (P<0.01) and tyrosinase activity significantly reduces (P<0.001)；Laminin332 and collagen
I positive cell numbers (P<0.01) dramatically increase.
Result above shows that 1~5 composition of the embodiment of the present invention can significantly reduce LEF-1, tyrosinase activity and carry
The expression quantity of high Laminin332 and collagen I, protects the skin from damage caused by UVB irradiations, with Whitening, spot
Effect.Wherein, the total addition level of embodiment 3 is minimum, but reaches the result similar with other embodiment.Therefore it is considered as most
Good adding proportion.
7 proteasome activity of embodiment detects
The present embodiment verifies light spot effect of anti-aging of the present composition by testing proteasome.Albumen
Enzyme body is that one of mechanism of misfolded protein is removed in mammalian cell, and the accumulation of misfolded protein directly results in
The aging of cell.
The extracting method of proteasome：
(1) from standard aged cells (contributor's age：62 years old) three kinds of proteasome subunits of middle separation.In 4 DEG C of environment
It is lower by cell extract centrifugation 16 it is small when (1000rcf).Sediment is dissolved in Tris-HCl buffer solutions (25mM, pH7.5),
It is then injected into the hydrogen bromide agarose (CNBr balanced in advance with buffer solution (Tris-HCl 25mM, pH 7.5)
Sepharose columns) and (containing can direct purification proteasome subunit monoclonal antibody) in.Then rushed using same buffer solution
The column is washed, the Tris-HCl solution elution proteasome containing 2M sodium chloride (NaCl) (pH 8) is reused, then in 4 DEG C of temperature
When the lower dialysis 16 of degree is small (or injection solvent resistant column (PD10 Sephadex)).
(2) proteasome sample purified with denaturation sample-loading buffer (SDS 0.1%) mixing, then in 100 DEG C of temperature
It is lower to be incubated 5 minutes.Then protein can be because acrylamide gel (12%) and sodium dodecyl sulfate polyacrylamide gel
Electrophoresis (SDS-PAGE) and separate.The migration of protein can pass through 80V voltage stabilizings in 30 minutes at room temperature, with 2 it is small when 120V voltage stabilizings in
Complete.
(3) final volume is contained into the thick homogenate of 50 μ g albumen or 3 μ g purifying protein enzyme bodies (in Tris-HCl for 200 μ l
25mM pH 7.5) mixture and lower floor be incubated 30 minutes at a temperature of 37 DEG C.Stopped using 100 μ l acid or ethanol anti-
Should.After adding 2ml distilled water, using the fluorescence of microplate reader measurement MCA, exciting light and wavelength of transmitted light are set as 350/440nm.
Experimental setup substrate group, experimental group 1~5：1 composition group of proteasome+embodiment, 2 groups of proteasome+embodiment
Compound group, 3 composition of proteasome+embodiment, 4 composition group of proteasome+embodiment, 5 composition of proteasome+embodiment
Group.The active constituent content that experimental group 1~5 is separately added into is the correspondent composition that mass fraction is 1%.
Test method：It is directly added into according to test concentrations after cultivating 24h in cell culture medium, extracts albumen as stated above
Enzyme body, MCA fluorescence is detected using microplate reader.Wherein, proteasome activity is defined as：The 20 μM of proteasomes suppressions of gross activity and addition
The difference of residual activity after preparation.It the results are shown in Table shown in 3.
3 proteasome activity of table detects
Note：* the p ＜ 0.05 compared with substrate group are represented.
The results show that the cell that 1~5 compositions-treated of the embodiment of the present invention is crossed, can recover naturally-aged cell (contributor
Age：62 years old) in proteasome subunit activity, compared with substrate group, there is significant difference (p ＜ 0.05).Wherein, implement
The total addition level of example 3 is minimum, but reaches the result similar with other embodiment.Therefore it is considered as coefficient optimum proportion.It is right
Ratio 1~5 is compared with substrate group, no significant difference.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of have whitening, the composition of light spot effect, it is characterised in that in parts by weight, including：
2. composition according to claim 1, it is characterised in that in parts by weight, including：
3. composition according to claim 1, it is characterised in that including：
4. according to the composition described in claims 1 to 3 any one, it is characterised in that the system of the hydrolysis conchiolin
Preparation Method is：
Step 1:Crushing pearl is taken, adds water to stir to obtain pearl slurry, adds lactic acid, react 1.5h~3h, mistake in 95~100 DEG C
Filter, collects filtrate and filter residue respectively；
Step 2：Take filter residue to add water, adjust pH value to 6.0~8.0, add neutral proteinase, 39~41 DEG C of enzyme digestion reactions 2.5~
4h, filtering, collects filtrate；
Step 3：The filtrate of combining step 1 and step 2, mixes, and centrifugation, freeze, and obtains hydrolysis conchiolin.
5. according to the composition described in claims 1 to 3 any one, it is characterised in that the preparation side of the Bulbus Lilii extract
Method is：
Using lily petal as explant, lily callus is obtained through Fiber differentiation, successively through secondary culture, Shaking culture, clearly
It is scattered in after washing in glycerine, obtains Bulbus Lilii extract.
6. according to the composition described in claims 1 to 3 any one, it is characterised in that the Caulis Bambusae In Taeniam extract, ganoderma lucidum extraction
Thing is raised by dictyophora phalloidea, ganoderma lucidum through water respectively.
7. according to the composition described in claims 1 to 3 any one, it is characterised in that the ginger-root extract is by race
Extract to obtain through 95% ethanol.
8. composition according to claim 7, it is characterised in that described to be extracted as sonic oscillation extraction.
9. composition as described in claim 1~8 any one prepare with whitening, light spot effect cosmetics in should
With.
10. a kind of have whitening, the skin care item of light spot effect, it is characterised in that by claim 1~8 any one of them group
Acceptable auxiliary material is made on compound and cosmetics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711476629.3A CN107898732B (en) | 2017-12-29 | 2017-12-29 | Composition with whitening and spot-fading effects, application thereof and skin care product |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711476629.3A CN107898732B (en) | 2017-12-29 | 2017-12-29 | Composition with whitening and spot-fading effects, application thereof and skin care product |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107898732A true CN107898732A (en) | 2018-04-13 |
| CN107898732B CN107898732B (en) | 2020-04-17 |
Family
ID=61872048
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711476629.3A Active CN107898732B (en) | 2017-12-29 | 2017-12-29 | Composition with whitening and spot-fading effects, application thereof and skin care product |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107898732B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110179702A (en) * | 2019-07-13 | 2019-08-30 | 山东效峰生物科技股份有限公司 | A kind of creams and its preparation process prepared using edible fungus extract |
| CN111514093A (en) * | 2020-05-18 | 2020-08-11 | 广西大海阳光药业有限公司 | Silver ion ethanol disinfectant and preparation method thereof |
| CN112294744A (en) * | 2020-10-28 | 2021-02-02 | 湖州欧利生物科技有限公司 | Preparation process of enzymolysis pearl freeze-dried powder |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102743322A (en) * | 2011-04-22 | 2012-10-24 | 上海家化联合股份有限公司 | Application of bamboo fungus extract in cosmetics |
| CN104000752A (en) * | 2013-02-27 | 2014-08-27 | 西姆莱斯股份公司 | Ginger extract for the protection of stem cells |
-
2017
- 2017-12-29 CN CN201711476629.3A patent/CN107898732B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102743322A (en) * | 2011-04-22 | 2012-10-24 | 上海家化联合股份有限公司 | Application of bamboo fungus extract in cosmetics |
| CN104000752A (en) * | 2013-02-27 | 2014-08-27 | 西姆莱斯股份公司 | Ginger extract for the protection of stem cells |
Non-Patent Citations (1)
| Title |
|---|
| COSDNA: "ZA（姬芮）true white esscence lotion", 《HTTP://WWW.COSDNA.COM/CHS/COSMETIC_F39B138353.HTML》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110179702A (en) * | 2019-07-13 | 2019-08-30 | 山东效峰生物科技股份有限公司 | A kind of creams and its preparation process prepared using edible fungus extract |
| CN110179702B (en) * | 2019-07-13 | 2022-01-04 | 山东效峰生物科技股份有限公司 | A cream prepared from edible fungus extract and its preparation method |
| CN111514093A (en) * | 2020-05-18 | 2020-08-11 | 广西大海阳光药业有限公司 | Silver ion ethanol disinfectant and preparation method thereof |
| CN112294744A (en) * | 2020-10-28 | 2021-02-02 | 湖州欧利生物科技有限公司 | Preparation process of enzymolysis pearl freeze-dried powder |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107898732B (en) | 2020-04-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102552099B (en) | Composite biological agent used for skin beautifying and restoring and preparing method | |
| US11684559B2 (en) | Topical cosmetic treatment of skin and scalp and corresponding active ingredient based on an extract of Apium graveolens | |
| CN1310032C (en) | Drugs for ameliorating itch, rough skin or hypersensitive skin or for whitening via inhibition of the production and release of stem cell | |
| CN102439027B (en) | Peptide hydrolysate proteasome activators and compositions containing same | |
| CN107898732A (en) | It is a kind of that there is whitening, the composition and its application, skin care item of light spot effect | |
| FR2970868A1 (en) | PLANT ORIGIN EXTRACT, COMPOSITION CONTAINING THE SAME, PROCESS FOR OBTAINING BY VEGETABLE CULTURE AND USES IN THE COSMETIC, PHARMACEUTICAL AND COSMECEUTICAL FIELDS | |
| CN111803408A (en) | Dischizosaccharomyces cerevisiae fermentation product filtrate and application thereof in skin care products | |
| CN107427457A (en) | Protect the skin from polluting and improve the cosmetic treatment method of skin regeneration | |
| KR20230147028A (en) | Composition for anti-aging, anti-inflammation or skin regeneration containing Cannabis sativa stem extract as effective component | |
| KR20190014477A (en) | Tremella fuciformis extraxt, preparation method thereof and Use the same | |
| CN102421412A (en) | Composition containing chamaecyparis obtusa polysaccharides to be externally applied to the skin | |
| KR101719124B1 (en) | Cosmetic composition for skin whitening and moisturing comprising natural minerals extract | |
| JP5856847B2 (en) | Skin external preparation composition containing Matsune extract | |
| CN111732634B (en) | Application of earthworm active peptide for inhibiting melanocyte proliferation in preparation of medicines or cosmetics | |
| JP2003073225A (en) | Cosmetic | |
| CN108066237A (en) | It is a kind of that there are whitening, the composition of light spot effect and its application, skin care item | |
| KR102065905B1 (en) | Composition for anti-aging comprising Bacillus pumilus strain or culture solution of the strain | |
| KR20210055475A (en) | A composition for improving skin beauty containing glycosaminoglycan | |
| KR20110033461A (en) | Cosmetic composition containing hedyotidis diffusae-stem cell extract for antioxidant and anti-aging | |
| KR101618726B1 (en) | Method for making protein from Pollock skin | |
| CN111012689B (en) | Preparation method of astragalus polypeptide and application of astragalus polypeptide in preparation of cosmetics | |
| CN106478770A (en) | A kind of Fructus Perillae oxidation resistance dipeptide and preparation method and application | |
| CN104497101B (en) | A kind of preparation method of wheat embryo cytoactive albumen | |
| EP0136352A1 (en) | Cosmetic composition and method for obtaining it. | |
| CN108042477A (en) | Muscle base fluid containing human adipose-derived mesenchymal stem cell extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Xiaoying Inventor after: Liu Guangrong Inventor after: Che Biao Inventor after: Tang Jian Inventor before: Liu Xiaoying Inventor before: Liu Guangrong Inventor before: Che Biao Inventor before: Tang Jian |
|
| CB03 | Change of inventor or designer information |