CN107897646A - A kind of method using laser assisted extraction corn yellow OB food additives - Google Patents
A kind of method using laser assisted extraction corn yellow OB food additives Download PDFInfo
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- CN107897646A CN107897646A CN201711164484.3A CN201711164484A CN107897646A CN 107897646 A CN107897646 A CN 107897646A CN 201711164484 A CN201711164484 A CN 201711164484A CN 107897646 A CN107897646 A CN 107897646A
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- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/40—Colouring or decolouring of foods
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Abstract
The present invention provides a kind of method using laser assisted extraction corn yellow OB food additives.Corn protein powder fine powder is made into suspension, cellulase is respectively adopted and neutral proteinase is digested, precipitation after enzymolysis liquid filtering is added in ethanol, assisted extraction is irradiated using laser, supernatant is taken after extracting solution filtering, protein is removed using flocculate with chitosan, and is concentrated under reduced pressure and vacuum freeze drying, obtains corn yellow OB food additives.This method can activate the enzyme in corn by laser irradiation, and thoroughly destroy cell membrane, discharge pigment to greatest extent, extraction efficiency is very fast, and yield is higher, and security is good, can be used as the colouring agent and additive of varieties of food items.Equipment and cost of material are low at the same time, are adapted to industrialized production.
Description
Technical field
The present invention relates to field of food, and in particular to the extraction of corn yellow OB, it is auxiliary using laser more particularly to one kind
The method for helping extraction corn yellow OB food additives.
Background technology
Corn is 1 year herbaceous plant of grass family Zea, has the characteristics of cultivated area is wide, and yield is big.In recent years, it is beautiful
The application of rice is increasingly extensive, and in terms of nutrition, corn capacity usage ratio is higher.Maize seed is yellow except liposoluble vitamin E and leaf
Cellulose content is higher, also has glutathione, has the function of anti-aging, strengthen immunity etc..At medicinal aspect, corn has drop
Pressure, hypoglycemic, prevent artery sclerosis and other effects.Therefore, the research and development of corn becomes domestic and international research hotspot.
Corn yellow OB is a kind of mixture of the containing oxygen derivative for several Carotenoids that can be extracted from corn,
Mainly it is made of luteole, lutern, kryptoxanthin and beta carotene, can be widely applied to the row such as medicine, food and packaging
Industry.Corn yellow OB belongs to different five classes dienes pigment, has important physiological function, such as suppresses the growth of tumour, the prevention heart
Cranial vascular disease, enhancing body immunity and prevention are senile regarding macular degeneration etc., thus develop and utilize nutrition in corn
Element-corn yellow OB has important research significance and economic value.
At present, the research to corn yellow OB focuses mostly in the extraction, purifying, stability of corn yellow OB, general natural
The extraction of pigment mostly uses chemical solvent extraction, and maize flavine is fat-soluble pigment, not soluble in water, is soluble in organic molten
Agent, therefore extract principle and mainly use traditional solvent extraction.Currently used method have enzyme process, microwave―assisted extraction,
Ultrasonic wave assisted extraction method, supercritical CO2Extraction etc., these methods can effectively improve the recovery rate of corn yellow OB, separately
The corn yellow OB of higher degree can be obtained by column chromatography, membrane separation technique and high speed adverse current chromatogram outside.It is right both at home and abroad
The research and application of the new and high technology Assisted Extraction of corn yellow OB achieve certain progress.
Chinese invention patent application number 201310045280.3 discloses one kind and corn yellow OB is extracted from zein
Method, comprise the following steps：Ultramicro-powder by corn protein powder ultramicro grinding into 300 mesh to 500 mesh, adds water, xylan
Enzyme and aspergillus oryzae protease enzymolysis, enzymolysis liquid centrifugation, abandons supernatant, must precipitate；Precipitation adds ethanol, in microwave reaction system
Extracted, collected supernatant；Supernatant removes protein, and filtrate decompression concentration, obtains paste, vacuum freeze drying to perseverance
Weight.
Chinese invention patent application number 201410280786.7 discloses a kind of method of microbial method extraction luteole,
The first step chooses corn grain slag, and second step carries out fermentation process, and the 3rd step is extracted with 95% alcohol, the concentration of the 4th step, and the 5th
Step carries out two step of the 4th step institute score, and a part carries out encapsulated embedding, carries out homogeneous, maize yellow made from atomization drying
Plain crude extract, another part macroporous resin purification, encapsulated embedding, homogeneous, spray drying, corn yellow OB extract.
Chinese invention patent application number 201510378672.0 discloses a kind of preparation method of luteole, and the invention will
Corn protein powder is dissolved in the water, and adds protease hydrolyzed；PH value is adjusted after being digested to corn protein powder；Centrifugal treating,
Supernatant is abandoned, what is obtained is precipitated as enzymolysis product；Enzymolysis product is extracted by organic solvent again, obtains containing luteole
Extract；Finally by rotary evaporation technique, luteole is obtained.
Chinese invention patent application number 201410600222.7 discloses a kind of preparation method of corn yellow OB, including with
Lower step：Corn protein powder is taken, adds digestion agent, when extraction 4 is small at 65 DEG C, then is filtered with Buchner funnel, repeats to soak
Carry 2 times, merging filtrate, centrifugation is evaporated under reduced pressure after removing impurity, is dried under vacuum to constant weight up to corn yellow OB.
According to above-mentioned, traditional solvent extraction is more demanding to solvent species and extraction conditions in existing scheme, at the same time
Effect is poor, and during existing new and high technology Assisted Extraction, there are some processing times length, it is complicated the problem of, and set
It is standby expensive and cost is higher, it is difficult to which that applied to industrialized production, it is relatively low to additionally there are extraction efficiency and yield, application range
The problems such as narrow and small, in consideration of it, the present invention proposes a kind of innovative utilization laser assisted extraction corn yellow OB food addition
The method of agent, can effectively solve above-mentioned technical problem.
The content of the invention
Reagent and extraction conditions are required for current application wider corn yellow OB solvent extraction high and effect compared with
Difference, while general new and high technology Assisted Extraction method, complicated there are processing time length, equipment is expensive and cost is higher
The problem of, and extraction efficiency and yield are relatively low, it is difficult to and applied to industrialized production, and there are extraction efficiency and yield are equal
Relatively low, application range is narrow and small, and the present invention proposes a kind of method using laser assisted extraction corn yellow OB food additives, from
And extraction efficiency has been effectively ensured, and it is suitable for large-scale promotion.
Concrete technical scheme of the present invention is as follows：
A kind of method using laser assisted extraction corn yellow OB food additives, the corn yellow OB food additives
Extraction process is：
（1）Use micronizer to crush corn protein powder for Ultramicro-powder, be stirred with water and be formulated as suspension, adjust pH
Value is adjusted the temperature to 53~57 DEG C, adds cellulase, carried out after digesting 1.5~2.5h using 1,2,3,-thrihydroxy-benzene to 8.5~9.5
Enzyme deactivation, then pH value is adjusted to 6.5~7.5,48~52 DEG C are adjusted the temperature to, adds neutral proteinase, 2.5~3.5h is digested, obtains
To enzymolysis liquid；The total mass fraction of the suspension in terms of 100 parts, wherein 10~20 parts of corn protein powder Ultramicro-powder, water 80~
90 parts；
（2）Must precipitate, enzymolysis liquid centrifugal filtration according to precipitation:The mass ratio of ethanol is 20~30:80~70, sink in gained
Ethanol is added in shallow lake, after mixing, the irradiation that moves in circles is carried out using laser, the cell of corn is thoroughly destroyed using laser
Wall, the enzyme in one-step activation corn of going forward side by side, makes various colour components farthest discharge, and be dissolved in ethanol, 20
Centrifugal filtration is carried out after~30min, and is collected supernatant；
（3）To step（2）Chitosan is added in the supernatant of collection, adjusts pH value to 5~6.3, temperature is 30~40 DEG C, with 40
The speed of~60r/min stirs 2~3min, stands 2~4h, under the action of a large amount of free amine groups of chitosan molecule, albumen
Flocculation sediment occurs for matter molecule, takes filtrate to be concentrated under reduced pressure after filtering, and vacuum freeze drying, you can corn yellow OB is made
Food additives；The addition of the chitosan is the 0.01~0.03% of supernatant quality.
Preferably, the particle diameter of the corn protein powder Ultramicro-powder is 0.5~1 μm.
Preferably, the cellulase is by circumscribed 1,4 beta-glucanase, Endo-β-glucanase, beta-glucosidase, wood
Dextranase, hemicellulase, amylase and protease composition.
Preferably, the neutral proteinase is yellowish-brown or brown powder, and moisture content is no more than 5%, and enzymatic activity is 10~30
Ten thousand U/g.
Preferably, the enzyme concentration of the cellulase is 1~1.5%；
Preferably, the enzyme concentration of the neutral proteinase is 0.8~1.2%.
Preferably, the centrifugal filtration process uses filtering centrifuge, can be link-suspended basket centrifuge, suspended centrifuge,
Scraper slag dumping formula centrifuge, piston push away one kind in slag formula centrifuge, spiral slag dumping formula centrifuge or vibration slag dumping formula centrifuge.
Preferably, the filtering centrifuge should select suitable roller diameter；The roller diameter of the link-suspended basket centrifuge
For 1000~1500mm；The roller diameter of the suspended centrifuge is 800~1200mm；The scraper slag dumping formula centrifuge
Roller diameter is 2000~2500mm；The roller diameter that the piston pushes away slag formula centrifuge is 500~800mm.
Preferably, the laser can be one kind in horizontal laser light instrument or vertical laser instrument；The laser can be pulse
One kind in laser or continuous laser；The power of the laser is 10~20mW.
Preferably, the temperature of the vacuum freeze drying is -20~-40 DEG C, and vacuum is 4~8Pa.
In the enzymolysis process of corn protein powder, since hydrophobic grouping contained in albumen powder is more, neutral proteinase pair
Its direct hydrolysis result is undesirable, and first can decompose most of hydrophobic group of corn protein powder using cellulase,
The water-soluble increase of albumen powder, may advantageously facilitate the effect of neutral proteinase.In enzymolysis process, the concentration pair of corn protein powder
Enzymolysis degree has an important influence on, and with the increase of concentration, moisture declines, and is unfavorable for the diffusion of enzyme molecule, make enzyme molecule with
Albumen powder it is insufficient contact, cause enzymolysis degree to decline, enzymolysis speed also declines.
Due to the directional lighting characteristic of laser, substantial amounts of photon is concentrated in a minimum spatial dimension, therefore laser
With very high energy density, when it is acted on the cell of corn protein powder, its cell membrane can be made thoroughly to be destroyed, cell
Matter escapes, and substantial amounts of pigment discharges.Meanwhile the enzyme inside the high-energy energy activator protein powder of laser, make its reactivity
Greatly enhance.Under the booster action of laser, the extraction efficiency of corn yellow OB is obviously improved, while recovery rate is also carried
It is high.
By the method for present invention extraction corn yellow OB, carried out with usual vehicle extraction, microwave or ultrasonic wave added extraction
Contrast, the method for the present invention have obvious advantage on time-consuming, yield, security and cost is extracted, as shown in table 1.
Table 1：
The present invention provides a kind of method using laser assisted extraction corn yellow OB food additives, with prior art phase
Than, its protrude the characteristics of and excellent effect be：
1st, the method using laser assisted extraction corn yellow OB food additives is proposed.
2nd, laser irradiation can activate the enzyme in corn, and be entirely destroyed the cell membrane of corn, various groups of pigment
Divide and discharge to greatest extent, accelerate extraction efficiency.
3rd, the yield of corn yellow OB is higher, and security is good, can be used as the colouring agent and additive of varieties of food items.
4th, extracting method of the invention, complicated, equipment and cost of material are low, are adapted to industrialized production.
Embodiment
In the following, the present invention will be further described in detail by way of specific embodiments, but this should not be interpreted as to the present invention
Scope be only limitted to following example.Without departing from the idea of the above method of the present invention, according to ordinary skill
The various replacements or change that knowledge and customary means are made, should be included in the scope of the present invention.
Embodiment 1
（1）Use micronizer to crush corn protein powder for Ultramicro-powder, be stirred with water and be formulated as suspension, adjust pH
Value adjusts the temperature to 53 DEG C to 9.5, adds cellulase, digests and carries out enzyme deactivation using 1,2,3,-thrihydroxy-benzene after 2h, then adjust pH value to
6.5,52 DEG C are adjusted the temperature to, adds neutral proteinase, digests 2.5h；The total mass fraction of the suspension in terms of 100 parts, its
Middle 20 parts of corn protein powder Ultramicro-powder, 80 parts of water；The particle diameter of corn protein powder Ultramicro-powder is 0.5 μm；The enzyme concentration of cellulase
For 1%；The enzyme concentration of neutral proteinase is 1.2%；
（2）By enzymolysis liquid centrifugal filtration, remove supernatant, ethanol is added in gained precipitation, after mixing, using laser
The irradiation that moves in circles is carried out, the cell membrane of corn is thoroughly destroyed using laser, the enzyme in one-step activation corn of going forward side by side, makes various colors
Plain component farthest discharges, and is dissolved in ethanol, centrifugal filtration is carried out after 30min, and collect supernatant；It is described
In ethanol extract, the total mass fraction of each component is in terms of 100 parts, wherein 30 parts of sediment of enzymolysis, 70 parts of ethanol；Filter centrifugation
Machine is link-suspended basket centrifuge；Laser is horizontal laser light instrument；The power of laser is 20mW；
（3）To step（2）Supernatant in add chitosan, adjust pH value to 5, temperature is 40 DEG C, is stirred with the speed of 40r/min
3min is mixed, stands 4h, under the action of a large amount of free amine groups of chitosan molecule, flocculation sediment, filtering occur for protein molecule
After take filtrate to be concentrated under reduced pressure, and vacuum freeze drying, you can corn yellow OB food additives are made；The chitosan
Addition is the 0.01% of supernatant quality；The temperature of vacuum freeze drying is -40 DEG C, vacuum 4Pa.
The method that embodiment 1 extracts corn yellow OB, it extracts total time-consuming, yield, foodsafety and extraction cost, such as
Shown in table 2.
Embodiment 2
（1）Use micronizer to crush corn protein powder for Ultramicro-powder, be stirred with water and be formulated as suspension, adjust pH
Value adjusts the temperature to 54 DEG C to 9, adds cellulase, digests and carries out enzyme deactivation using 1,2,3,-thrihydroxy-benzene after 1.5h, then adjust pH value to
6.5,52 DEG C are adjusted the temperature to, adds neutral proteinase, digests 3.5h；The total mass fraction of the suspension in terms of 100 parts, its
Middle 15 parts of corn protein powder Ultramicro-powder, 85 parts of water；The particle diameter of corn protein powder Ultramicro-powder is 0.8 μm；The enzyme concentration of cellulase
For 1.4%；The enzyme concentration of neutral proteinase is 1.1%；
（2）By enzymolysis liquid centrifugal filtration, remove supernatant, ethanol is added in gained precipitation, after mixing, using laser
The irradiation that moves in circles is carried out, the cell membrane of corn is thoroughly destroyed using laser, the enzyme in one-step activation corn of going forward side by side, makes various colors
Plain component farthest discharges, and is dissolved in ethanol, centrifugal filtration is carried out after 25min, and collect supernatant；It is described
In ethanol extract, the total mass fraction of each component is in terms of 100 parts, wherein 28 parts of sediment of enzymolysis, 72 parts of ethanol；Filter centrifugation
Machine is suspended centrifuge；Laser is vertical laser instrument；The power of laser is 18mW；
（3）To step（2）Supernatant in add chitosan, adjust pH value to 6.3, temperature is 32 DEG C, with the speed of 48r/min
3min is stirred, stands 2h, under the action of a large amount of free amine groups of chitosan molecule, flocculation sediment, mistake occur for protein molecule
Filtrate is taken to be concentrated under reduced pressure after filter, and vacuum freeze drying, you can corn yellow OB food additives are made；The chitosan
Addition be supernatant quality 0.02%；The temperature of vacuum freeze drying is -20 DEG C, vacuum 8Pa.
The method that embodiment 2 extracts corn yellow OB, it extracts total time-consuming, yield, foodsafety and extraction cost, such as
Shown in table 2.
Embodiment 3
（1）Use micronizer to crush corn protein powder for Ultramicro-powder, be stirred with water and be formulated as suspension, adjust pH
Value adjusts the temperature to 53 DEG C, adds cellulase, use 1,2,3,-thrihydroxy-benzene to carry out enzyme deactivation after digesting 1.5h, then adjust pH value to 9.5
To 7.5,49 DEG C are adjusted the temperature to, adds neutral proteinase, digests 3h；The total mass fraction of the suspension in terms of 100 parts, its
Middle 12 parts of corn protein powder Ultramicro-powder, 88 parts of water；The particle diameter of corn protein powder Ultramicro-powder is 0.6 μm；The enzyme concentration of cellulase
For 1.2%；The enzyme concentration of neutral proteinase is 1.2%；
（2）By enzymolysis liquid centrifugal filtration, remove supernatant, ethanol is added in gained precipitation, after mixing, using laser
The irradiation that moves in circles is carried out, the cell membrane of corn is thoroughly destroyed using laser, the enzyme in one-step activation corn of going forward side by side, makes various colors
Plain component farthest discharges, and is dissolved in ethanol, centrifugal filtration is carried out after 22min, and collect supernatant；It is described
In ethanol extract, the total mass fraction of each component is in terms of 100 parts, wherein 28 parts of sediment of enzymolysis, 72 parts of ethanol；Filter centrifugation
Machine is scraper slag dumping formula centrifuge；Laser is horizontal laser light instrument；The power of laser is 15mW；
（3）To step（2）Supernatant in add chitosan, adjust pH value to 6, temperature is 35 DEG C, is stirred with the speed of 55r/min
3min is mixed, stands 3h, under the action of a large amount of free amine groups of chitosan molecule, flocculation sediment, filtering occur for protein molecule
After take filtrate to be concentrated under reduced pressure, and vacuum freeze drying, you can corn yellow OB food additives are made；The chitosan
Addition is the 0.03% of supernatant quality；The temperature of vacuum freeze drying is -30 DEG C, vacuum 5Pa.
The method that embodiment 3 extracts corn yellow OB, it extracts total time-consuming, yield, foodsafety and extraction cost, such as
Shown in table 2.
Embodiment 4
（1）Use micronizer to crush corn protein powder for Ultramicro-powder, be stirred with water and be formulated as suspension, adjust pH
Value adjusts the temperature to 57 DEG C, adds cellulase, use 1,2,3,-thrihydroxy-benzene to carry out enzyme deactivation after digesting 1.5h, then adjust pH value to 8.5
To 7.5,48 DEG C are adjusted the temperature to, adds neutral proteinase, digests 3.5h；The total mass fraction of the suspension in terms of 100 parts,
Wherein 20 parts of corn protein powder Ultramicro-powder, 80 parts of water；The particle diameter of corn protein powder Ultramicro-powder is 0.5 μm；Cellulase it is enzyme
Measure as 1.5%；The enzyme concentration of neutral proteinase is 0.8%；
（2）By enzymolysis liquid centrifugal filtration, remove supernatant, ethanol is added in gained precipitation, after mixing, using laser
The irradiation that moves in circles is carried out, the cell membrane of corn is thoroughly destroyed using laser, the enzyme in one-step activation corn of going forward side by side, makes various colors
Plain component farthest discharges, and is dissolved in ethanol, centrifugal filtration is carried out after 20min, and collect supernatant；It is described
In ethanol extract, the total mass fraction of each component is in terms of 100 parts, wherein 20 parts of sediment of enzymolysis, 80 parts of ethanol；Filter centrifugation
Machine pushes away slag formula centrifuge for piston；Laser is vertical laser instrument；The power of laser is 20mW；
（3）To step（2）Supernatant in add chitosan, adjust pH value to 5, temperature is 40 DEG C, is stirred with the speed of 45r/min
3min is mixed, stands 4h, under the action of a large amount of free amine groups of chitosan molecule, flocculation sediment, filtering occur for protein molecule
After take filtrate to be concentrated under reduced pressure, and vacuum freeze drying, you can corn yellow OB food additives are made；The chitosan
Addition is the 0.01% of supernatant quality；The temperature of vacuum freeze drying is -20 DEG C, vacuum 6Pa.
The method that embodiment 4 extracts corn yellow OB, it extracts total time-consuming, yield, foodsafety and extraction cost, such as
Shown in table 2.
Embodiment 5
（1）Use micronizer to crush corn protein powder for Ultramicro-powder, be stirred with water and be formulated as suspension, adjust pH
Value adjusts the temperature to 55 DEG C, adds cellulase, use 1,2,3,-thrihydroxy-benzene to carry out enzyme deactivation after digesting 2.5h, then adjust pH value to 8.8
To 6.5,51 DEG C are adjusted the temperature to, adds neutral proteinase, digests 2.5h；The total mass fraction of the suspension in terms of 100 parts,
Wherein 10 parts of corn protein powder Ultramicro-powder, 90 parts of water；The particle diameter of corn protein powder Ultramicro-powder is 1 μm；The enzyme concentration of cellulase
For 1%；The enzyme concentration of neutral proteinase is 1%；
（2）By enzymolysis liquid centrifugal filtration, remove supernatant, ethanol is added in gained precipitation, after mixing, using laser
The irradiation that moves in circles is carried out, the cell membrane of corn is thoroughly destroyed using laser, the enzyme in one-step activation corn of going forward side by side, makes various colors
Plain component farthest discharges, and is dissolved in ethanol, centrifugal filtration is carried out after 28min, and collect supernatant；It is described
In ethanol extract, the total mass fraction of each component is in terms of 100 parts, wherein 25 parts of sediment of enzymolysis, 75 parts of ethanol；Filter centrifugation
Machine is spiral slag dumping formula centrifuge；Laser is horizontal laser light instrument；The power of laser is 20mW；
（3）To step（2）Supernatant in add chitosan, adjust pH value to 6, temperature is 36 DEG C, is stirred with the speed of 48r/min
3min is mixed, stands 3h, under the action of a large amount of free amine groups of chitosan molecule, flocculation sediment, filtering occur for protein molecule
After take filtrate to be concentrated under reduced pressure, and vacuum freeze drying, you can corn yellow OB food additives are made；The chitosan
Addition is the 0.03% of supernatant quality；The temperature of vacuum freeze drying is -40 DEG C, vacuum 4Pa.
The method that embodiment 5 extracts corn yellow OB, it extracts total time-consuming, yield, foodsafety and extraction cost, such as
Shown in table 2.
Embodiment 6
（1）Use micronizer to crush corn protein powder for Ultramicro-powder, be stirred with water and be formulated as suspension, adjust pH
Value adjusts the temperature to 53 DEG C, adds cellulase, use 1,2,3,-thrihydroxy-benzene to carry out enzyme deactivation after digesting 2.5h, then adjust pH value to 9.5
To 6.5,48 DEG C are adjusted the temperature to, adds neutral proteinase, digests 3.5h；The total mass fraction of the suspension in terms of 100 parts,
Wherein 20 parts of corn protein powder Ultramicro-powder, 80 parts of water；The particle diameter of corn protein powder Ultramicro-powder is 1 μm；The enzyme concentration of cellulase
For 1%；The enzyme concentration of neutral proteinase is 1.2%；
（2）By enzymolysis liquid centrifugal filtration, remove supernatant, ethanol is added in gained precipitation, after mixing, using laser
The irradiation that moves in circles is carried out, the cell membrane of corn is thoroughly destroyed using laser, the enzyme in one-step activation corn of going forward side by side, makes various colors
Plain component farthest discharges, and is dissolved in ethanol, centrifugal filtration is carried out after 20min, and collect supernatant；It is described
In ethanol extract, the total mass fraction of each component is in terms of 100 parts, wherein 30 parts of sediment of enzymolysis, 70 parts of ethanol；Filter centrifugation
Machine is vibration slag dumping formula centrifuge；Laser is vertical laser instrument；The power of laser is 10mW；
（3）To step（2）Supernatant in add chitosan, adjust pH value to 6.3, temperature is 30 DEG C, with the speed of 60r/min
2min is stirred, stands 4h, under the action of a large amount of free amine groups of chitosan molecule, flocculation sediment, mistake occur for protein molecule
Filtrate is taken to be concentrated under reduced pressure after filter, and vacuum freeze drying, you can corn yellow OB food additives are made；The chitosan
Addition be supernatant quality 0.01%；The temperature of vacuum freeze drying is -40 DEG C, vacuum 6Pa.
The method that embodiment 6 extracts corn yellow OB, it extracts total time-consuming, yield, foodsafety and extraction cost, such as
Shown in table 2.
Comparative example 1
（1）Use micronizer to crush corn protein powder for Ultramicro-powder, be stirred with water and be formulated as suspension, adjust pH
Value adjusts the temperature to 53 DEG C, adds cellulase, use 1,2,3,-thrihydroxy-benzene to carry out enzyme deactivation after digesting 2.5h, then adjust pH value to 9.5
To 6.5,48 DEG C are adjusted the temperature to, adds neutral proteinase, digests 3.5h；The total mass fraction of the suspension in terms of 100 parts,
Wherein 20 parts of corn protein powder Ultramicro-powder, 80 parts of water；The particle diameter of corn protein powder Ultramicro-powder is 1 μm；The enzyme concentration of cellulase
For 1%；The enzyme concentration of neutral proteinase is 1.2%；
（2）By enzymolysis liquid centrifugal filtration, remove supernatant, ethanol is added in gained precipitation, after mixing, stirring is soaked
Carry, centrifugal filtration is carried out after 14h, and collect supernatant；In the ethanol extract, the total mass fraction of each component is with 100 parts
Meter, wherein 30 parts of sediment of enzymolysis, 70 parts of ethanol；Filtering centrifuge is vibration slag dumping formula centrifuge；
（3）To step（2）Supernatant in add chitosan, adjust pH value to 6.3, temperature is 30 DEG C, with the speed of 60r/min
2min is stirred, stands 4h, under the action of a large amount of free amine groups of chitosan molecule, flocculation sediment, mistake occur for protein molecule
Filtrate is taken to be concentrated under reduced pressure after filter, and vacuum freeze drying, you can corn yellow OB food additives are made；The chitosan
Addition be supernatant quality 0.01%；The temperature of vacuum freeze drying is -40 DEG C, vacuum 6Pa.
The method that comparative example 1 extracts corn yellow OB, it extracts total time-consuming, yield, foodsafety and extraction cost, such as
Shown in table 2.
Table 2：
Claims (8)
- A kind of 1. method using laser assisted extraction corn yellow OB food additives, it is characterised in that the maize yellow The extraction process of vegetable food additive is：（1）Use micronizer to crush corn protein powder for Ultramicro-powder, be stirred with water and be formulated as suspension, adjust pH Value is adjusted the temperature to 53~57 DEG C, adds cellulase, carried out after digesting 1.5~2.5h using 1,2,3,-thrihydroxy-benzene to 8.5~9.5 Enzyme deactivation, then pH value is adjusted to 6.5~7.5,48~52 DEG C are adjusted the temperature to, adds neutral proteinase, 2.5~3.5h is digested, obtains To enzymolysis liquid；The total mass fraction of the suspension in terms of 100 parts, wherein 10~20 parts of corn protein powder Ultramicro-powder, water 80~ 90 parts；（2）Must precipitate, enzymolysis liquid centrifugal filtration according to precipitation:The mass ratio of ethanol is 20~30:80~70, sink in gained Ethanol is added in shallow lake, after mixing, the irradiation that moves in circles is carried out using laser, the cell of corn is thoroughly destroyed using laser Wall, the enzyme in one-step activation corn of going forward side by side, makes various colour components farthest discharge, and be dissolved in ethanol, 20 Centrifugal filtration is carried out after~30min, and is collected supernatant；（3）To step（2）Chitosan is added in the supernatant of collection, adjusts pH value to 5~6.3, temperature is 30~40 DEG C, with 40 The speed of~60r/min stirs 2~3min, stands 2~4h, under the action of a large amount of free amine groups of chitosan molecule, albumen Flocculation sediment occurs for matter molecule, takes filtrate to be concentrated under reduced pressure after filtering, and vacuum freeze drying, you can corn yellow OB is made Food additives；The addition of the chitosan is the 0.01~0.03% of supernatant quality.
- 2. a kind of method using laser assisted extraction corn yellow OB food additives according to claim 1, its feature It is：The particle diameter of the corn protein powder Ultramicro-powder is 0.5~1 μm.
- 3. a kind of method using laser assisted extraction corn yellow OB food additives according to claim 1, its feature It is：The cellulase is by circumscribed 1,4 beta-glucanase, Endo-β-glucanase, beta-glucosidase, zytase, half fiber The plain enzyme of dimension, amylase and protease composition.
- 4. a kind of method using laser assisted extraction corn yellow OB food additives according to claim 1, its feature It is：The neutral proteinase is yellowish-brown or brown powder, and moisture content is no more than 5%, and enzymatic activity is 10~300,000 U/g.
- 5. a kind of method using laser assisted extraction corn yellow OB food additives according to claim 1, its feature It is：The enzyme concentration of the cellulase is 1~1.5%；The enzyme concentration of the neutral proteinase is 0.8~1.2%.
- 6. a kind of method using laser assisted extraction corn yellow OB food additives according to claim 1, its feature It is：The centrifugal filtration process uses filtering centrifuge, is link-suspended basket centrifuge, suspended centrifuge, the centrifugation of scraper slag dumping formula Machine, piston push away one kind in slag formula centrifuge, spiral slag dumping formula centrifuge or vibration slag dumping formula centrifuge；The filtering centrifuge should select suitable roller diameter；The roller diameter of the link-suspended basket centrifuge for 1000~ 1500mm；The roller diameter of the suspended centrifuge is 800~1200mm；The roller diameter of the scraper slag dumping formula centrifuge For 2000~2500mm；The roller diameter that the piston pushes away slag formula centrifuge is 500~800mm.
- 7. a kind of method using laser assisted extraction corn yellow OB food additives according to claim 1, its feature It is：The laser is one kind in horizontal laser light instrument or vertical laser instrument；The laser is pulse laser or continuous laser In one kind；The power of the laser is 10~20mW.
- 8. a kind of method using laser assisted extraction corn yellow OB food additives according to claim 1, its feature It is：The temperature of the vacuum freeze drying is -20~-40 DEG C, and vacuum is 4~8Pa.
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| CN110256319A (en) * | 2019-07-03 | 2019-09-20 | 齐鲁工业大学 | A method of extracting luteole from the corn protein powder of enzymatic hydrolysis |
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