CN107897504A - Swamp eel tames and dociles the purposes of food phase special feed - Google Patents
Swamp eel tames and dociles the purposes of food phase special feed Download PDFInfo
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- CN107897504A CN107897504A CN201711237674.3A CN201711237674A CN107897504A CN 107897504 A CN107897504 A CN 107897504A CN 201711237674 A CN201711237674 A CN 201711237674A CN 107897504 A CN107897504 A CN 107897504A
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
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Abstract
The invention discloses the purposes that swamp eel tames and dociles food phase special feed, 80 100 parts of freshwater mussel powders, 30 40 parts of fish oil nano-emulsions, 10 12 parts of Meretrix meretrix Glycopeptides, 30 50 portions of earthworms, 30 40 parts of fly maggots, 15 30 parts of freshwater lobsters, 50 60 parts of fresh fish, 10 15 parts of Yolk antibodies, 58 parts of sodium butyrate mixing granulations are obtained into swamp eel and tame and docile food phase special feed, swamp eel is tamed and dociled into food phase special feed and tame and docile food of the man-made feeds cooperation for swamp eel seedling.Have the beneficial effect that：Swamp eel tames and dociles the tame and docile food that food phase special feed is applied to swamp eel seedling, greatly reduces the stress damage of swamp eel seedling and infects the probability of pest and disease damage, while substantially reduces the tame and docile phase in eclipse cycle, improves breeding efficiency, reduces feeding cost.
Description
Technical field
The present invention relates to swamp eel to tame and docile food field of fodder, and the purposes of food phase special feed is tamed and dociled more particularly, to swamp eel.
Technical background
Swamp eel, also known as yellow eel, close a kind of fish that gill Gyrinocheilidae swamp eel belongs to.Body is elongated serpentine-like, and body is about 20.70 centimetres,
It is most long 1 meter reachable.Circle rear portion is flat-sided before body, and tail point is thin, long and justify, and mouth is big, holds position, and the upper jaw is slightly prominent, and lip is quite flourishing.Up and down
There is serration on jaw and palatine bone.Eye is small, is covered by a thin skin.The thick tail of head is thin, and body surface has one layer of smooth mucous membrane protection to go,
Without squama, color and luster yellowish-brown, body then has irregular black dull spot, the undeveloped basic disappearance of each fin, and whole body only has a trigone thorn,
The few meat of thorn is thick, and meat tenderness is delicious.The Muddy Bottoms bottom such as rice field, river, brook, pond, rivers and canals, lake layer often is lived in, in China
There are production in various regions, more with Yangtze River in China basin, Liaoning and Tianjin yield, and term is produced most with the 6-8 months in the 6-10 months
Fertilizer.Widely distributed what east Asia and nearby big small island, west from Southeast Asia, to the east of the Philippines, North gets Japan, south is extremely
East Indies.
Swamp eel is because it has higher edible and medical value, and at home and abroad in the market sales volume is big, price is high.Due to natural money
Source is increasingly reduced, and the breeding of its fingerlings artificial is difficult to realize scale again, and high-quality seed is chosen in natural water area and is manually supported
Growing just becomes a hot spot of famous-particular-excellent aquaculture.Swamp eel is very strong to the selectivity of bait, once get used to eating a kind of feed
Not malleable afterwards, thus its artificial breeding tame and docile eat it is successfully whether very crucial.
The prior art discloses a kind of swamp eel and tames and dociles food as authorized the Chinese invention patent that public platform is 102273571 B of CN
Feed, the tame and docile food feed in the invention are formulated as following weight percent：Silver carp 20-30%, earthworm 12-38%, fly maggot 10-
20%, artifical compound feed 10-50%.By three phase feedings, animal food feed ingredient is gradually reduced, improves artifical compound feed
Component, improves the domestication effect of swamp eel.The invention targetedly tames the wild swamp eel of animal food according to the physiological property of swamp eel
To adapt to the swamp eel of artifical compound feed raising, the feed formula is non-to be subjected to liking for swamp eel.But in the inventive method
Silver carp, earthworm, fly maggot are fed at once without retort sterilization gives swamp eel seedling, and swamp eel seedling can be made to infect parasitic disease, influences
The health of swamp eel seedling, and the tame and docile food excessive cycle in the inventive method, influence to tame and docile food effect.
The content of the invention
It is an object of the invention to provide the purposes that swamp eel tames and dociles food phase special feed, swamp eel tames and dociles food phase special feed can be big
The immunocompetence and anti-stress ability of big enhancing swamp eel seedling, promote swamp eel seedling safety to spend the tame and docile food phase.
Used microorganism and its bought enterprise are in the method for the present invention：Aspergillus oryzae, Shandong Changtai biotechnology
Co., Ltd.
The present invention is directed to the problem of being mentioned in background technology, and the technical solution taken is：Swamp eel tames and dociles food phase special feed
Purposes, swamp eel, which tames and dociles food phase special feed and can be applied to swamp eel seedling, to be tamed and dociled the food phase, helps swamp eel seedling safety to spend the tame and docile food phase, is had
Gymnastics conduct：By swamp eel seedling be transferred to plantation have water plant and continue oxygen supply native pond in, oxygen supply exhausting-gas hole should leave pool wall 30-
50cm, the food platform being laid at 1 with water plant must be set per 4-6 square metres of area, and food platform is laid with water plant and formed；Enter pond to stop eating 1-
Start to tame and docile food after 2 days, select to tame during the 45-60 minutes turned on light after the sun sets, while to turn off water pump, tame and docile food first
The feeding volume in week is the 3-4% of swamp eel seedling weight, and it is by weight 9-2 that swamp eel is tamed and dociled food phase special feed with man-made feeds:1
Carry out mixing to feed, the accounting of man-made feeds increases successively, tames and dociles food and is changed to eat for one day 2 after 3-4 days, feeds standard as the second meal nothing
Surplus bait；The feeding volume for taming and dociling food second week is the 5-8% of swamp eel weight, and swamp eel is tamed and dociled food phase special feed and man-made feeds by weight
Than for 2-0:1, which carries out mixing, feeds, and the accounting of man-made feeds increases successively, and taming and dociling food can terminate to tame and docile the food phase for 2 weeks；Eat the water at platform
Grass is thick close, and to there is the yellow eel of enough buoyancy support bait and food, oxygen supply exhausting-gas hole should leave pool wall 30-50cm, prevent
Swamp eel seedling is gathered in pool wall and causes to abrade；Swamp eel tames and dociles the tame and docile food that food phase special feed is applied to swamp eel seedling, greatly reduces
The stress damage of swamp eel seedling and the probability for infecting pest and disease damage, while the tame and docile phase in eclipse cycle is substantially reduced, breeding efficiency is improved,
Reduce feeding cost.
Preferably, the preparation method that swamp eel tames and dociles food phase special feed includes：Fermentation, fish oil is modified, prepares fish oil nanometer
Breast, the preparation of Meretrix meretrix Glycopeptide, boiling freshwater mussel meat, compounding, specifically include following steps：
Fermentation：The aspergillus oryzae seed liquor of 3-5% is inoculated with fermentation tank, training of fermenting under the conditions of 30-32 DEG C, 180-200r/min
Support 30-36 it is small when, be filtered to remove nutrient solution, be washed with distilled water collection mycelium after 3-4 time, phosphoric acid is added after being filtered dry distilled water
Salt buffer soaks 20-30 minutes, the vacuum freeze drying under the conditions of -50~-60 DEG C, by obtained aspergillus oryzae filament in 0-
4 DEG C of refrigerators save backup；Fermentation prepares that aspergillus oryzae filament method is simple and practicable, and mycelia yield is higher, is further fish
Oil modification is prepared；
Fish oil is modified：Molar ratio 1 is added in a reservoir:The glyceride type fish oil and ethyl ester type fish oil of 2.8-3.0, is adding fish oil
The aspergillus oryzae filament of gross mass 4-5%, adds the functional polypeptide of aspergillus oryzae mycelium weight 1.0-1.3%, seals postposition
On the water bath with thermostatic control shaking table of 68-70 DEG C of temperature and 150-160r/min rotating speeds, reaction 18-20 it is small when after Cord blood must be modified
Fish oil；The amino acid sequence of functional polypeptide is：SCASRCKSRCRARRCRARRCGGGICKYFGKRCICKCFRC；Feature
Polypeptide can act on the reaction of aspergillus oryzae filament and ethyl ester type fish oil, promotes the accounting of EPA and DHA in reaction product, carries
The nutritive value of high product, the EPA and DHA of high-content help to improve the lipid structure in swamp eel seedling body, enhancing swamp eel children
The constitution of seedling；Microbial enzyme method has high catalytic efficiency, high selectivity, the advantages such as energy consumption is low in industrialized production, microorganism
Enzyme process reaction condition is gentle, and the structure of n-3 PUFAs will not be damaged, and can greatly improve ethyl ester type fish oil in product
The content of EPA and DHA, enzymic catalytic reaction can carry out in solvent-free system, so as to avoid huge production equipment, production peace
Overall coefficient higher；
Prepare fish oil nano-emulsion：Fish oil and whey protein isolate are weighed respectively, dissolve breast with the phosphate buffer of pH7.0-7.2
Clear protein isolate, prepares the whey protein isolate solution of 25-28g/L, after adding 95-100g/L fish oil while stirring, in 4-10
At a temperature of DEG C thick lotion is obtained in 20000-25000r/min speed conditions for emulsified 100-120 seconds through high-speed shearing emulsion machine, will
Thick lotion circulate homogenization under 80-120MPa pressure and obtains fish oil nano-emulsion 4-5 times；Fish oil can be carried significantly through nano-emulsion embedding
High fish oil oxidation stability, HIGH PRESSURE TREATMENT are conducive to improve the stability of fish oil nano-emulsion；
The preparation of Meretrix meretrix Glycopeptide：Take clam through spit it is husky, shell, freeze, crushes after acquisition clam dry powder, by solid-liquid ratio 1:8-10 is molten
In distilled water and it is homogenized, is centrifuged 25-30 minutes in 1-4 DEG C, 1000-2000r/min, collect supernatant, then surpass through doughnut
Filter column ultrafiltration, ultrafiltration column molecular cut off are 8 × 104, small-molecular-weight ultrafiltrate is collected in ultrafiltration, in -20 ~ -40 DEG C of preservations；Super
The chloroform of 1/5-2/5 volumes and the n-butanol of 1/25-1/20 volumes are added in filtrate, is extracted 3-4 times；Extract is dialysed, from
Meretrix meretrix Glycopeptide crude product is obtained after the heart, vacuum drying, centrifuging and taking supernatant after crude product is water-soluble, then with 0-1.0mol/L sodium chloride solutions ladder
Degree elution, obtains Meretrix meretrix Glycopeptide；The purity of gained Meretrix meretrix Glycopeptide is more than 96%, sugar and protein content each 54% and 42%, clam
Glycopeptide has excellent antitumor activity, bacteriostasis antibiosis activity, can effectively strengthen the immunocompetence of close octopus, strengthen close octopus constitution
With vigor, incubation rate is improved；
Boiling freshwater mussel meat：Take freshwater mussel to be smashed to pieces after spitting husky, cleaning, add the fish meat emulsion of 30-50% weight, add 2-3 times of water, then
The salt of dry weight 3-5% is added, postcooling when thermophilic digestion 1-2 is small, freshwater mussel powder is obtained after low temperature drying and crushing；Thermophilic digestion can
By the microorganism in freshwater mussel meat, fish meat emulsion, parasite killing, to prevent from damaging to swamp eel seedling, while thermophilic digestion can be with
Increase freshwater mussel meat, the fragrance of fish meat emulsion, increase attractant and palatability；
Compounding：Weigh 80-100 parts of freshwater mussel powders, 30-40 parts of fish oil nano-emulsions, 10-12 parts of Meretrix meretrix Glycopeptides, 30-50 parts of earthworms, 30-
40 parts of fly maggots, 15-30 parts of freshwater lobsters, 50-60 parts of fresh fish, 10-15 parts of Yolk antibodies, 5-8 parts of sodium butyrates, after mixing powder
It is broken, 50-80 mesh sieves are crossed after low temperature drying and crushing, is granulated on wet granulator, particle diameter 0.5-3.0mm, food is tamed and dociled up to swamp eel
Phase special feed；It is full of nutrition that swamp eel tames and dociles food phase special feed, while has enhancing swamp eel seedling immunocompetence and resisting stress energy
The effect of power, can greatly enhance seedling resistance, stop extraneous pathogenic microorganisms invasion and attack, and there is feed strong food calling to make
With, by increasing capacitance it is possible to increase the food ration of seedling, the nutritional ingredient enriched in feed can accelerate metabolism, stimulate the secretion of mucus,
Swamp eel seedling safety is set to spend the tame and docile food phase.
Compared with prior art, the advantage of the invention is that：1）Functional polypeptide can act on aspergillus oryzae filament with
The reaction of ethyl ester type fish oil, promotes the accounting of EPA and DHA in reaction product, improves the nutritive value of product, the EPA of high-content
Contribute to improve the lipid structure in swamp eel seedling body with DHA, strengthen the constitution of swamp eel seedling；2）Swamp eel tames and dociles food phase special feed
It is full of nutrition, while have the function that to strengthen swamp eel seedling immunocompetence and anti-stress ability, seedling resistance can be greatly enhanced
Power, stops extraneous pathogenic microorganisms invasion and attack, and feed has strong attractant effect, by increasing capacitance it is possible to increase the food ration of seedling, in feed
Abundant nutritional ingredient can accelerate metabolism, stimulate the secretion of mucus, swamp eel seedling safety is spent the tame and docile food phase；3）Swamp eel
The tame and docile food that food phase special feed is applied to swamp eel seedling is tamed and dociled, greatly reduces the stress damage and infection pest and disease damage of swamp eel seedling
Probability, while substantially reduce the tame and docile phase in eclipse cycle, improve breeding efficiency, reduce feeding cost.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
Swamp eel tames and dociles the purposes of food phase special feed, and swamp eel, which tames and dociles food phase special feed and can be applied to swamp eel seedling, to be tamed and dociled the food phase, is helped
Swamp eel seedling safety spends the tame and docile food phase, and concrete operations are：
Swamp eel seedling is transferred to plantation has water plant and continues in the native pond of oxygen supply, and oxygen supply exhausting-gas hole should leave pool wall 30cm, and every 4
Square metre area must set the food platform being laid at 1 with water plant, and food platform is laid with water plant and formed；
Enter and start to tame and docile food after pond is stopped eating 1 day, select to tame during 45 minutes to turn on light after the sun sets, while to turn off water
Pump, it is the 3% of swamp eel seedling weight to tame and docile the food feeding volume of first week, and swamp eel is tamed and dociled food phase special feed and man-made feeds by weight
Than for 9-2:1, which carries out mixing, feeds, and the accounting of man-made feeds increases successively, tames and dociles food and is changed to eat for one day 2 after 3 days, the standard of feeding is
Second meal is without surplus bait；
The feeding volume for taming and dociling food second week is the 5% of swamp eel weight, and swamp eel is tamed and dociled food phase special feed is by weight with man-made feeds
2-0:1, which carries out mixing, feeds, and the accounting of man-made feeds increases successively, and taming and dociling food can terminate to tame and docile the food phase for 2 weeks；The water plant eaten at platform will
Thick close, to there is the yellow eel of enough buoyancy support bait and food, oxygen supply exhausting-gas hole should leave pool wall 30-50cm, prevent swamp eel
Seedling is gathered in pool wall and causes to abrade；Swamp eel tames and dociles the tame and docile food that food phase special feed is applied to swamp eel seedling, greatly reduces swamp eel
The stress damage of seedling and the probability for infecting pest and disease damage, while the tame and docile phase in eclipse cycle is substantially reduced, breeding efficiency is improved, is reduced
Feeding cost.
Embodiment 2：
The preparation method that swamp eel tames and dociles food phase special feed includes：Comprise the following steps：
1）The aspergillus oryzae seed liquor of inoculation 3% in fermentation tank, when fermented and cultured 30 is small under the conditions of 30 DEG C, 180r/min, filtering
Nutrient solution is removed, mycelium is collected after being washed with distilled water 3 times, 20 points of phosphate buffer immersion is added after being filtered dry distilled water
Clock, the vacuum freeze drying under the conditions of -50 DEG C, obtained aspergillus oryzae filament is saved backup in 0 DEG C of refrigerator；2）In a reservoir
Add molar ratio 1:2.8 glyceride type fish oil and ethyl ester type fish oil, is adding the aspergillus oryzae filament of fish oil gross mass 4%, then
The functional polypeptide of aspergillus oryzae mycelium weight 1.0% is added, sealing is placed on the thermostatted water of 68 DEG C of temperature and 150r/min rotating speeds
Bathe shaking table on, reaction 18 it is small when after Cord blood obtain Modified Fish Oil；3）Fish oil and whey protein isolate are weighed respectively, use pH7.0
Phosphate buffer dissolving whey protein isolate, prepare the whey protein isolate solution of 25g/L, add 95g/L while stirring
After fish oil, thick lotion is obtained in 20000r/min speed conditions within emulsified 100 seconds at a temperature of 4 DEG C through high-speed shearing emulsion machine, will
Thick lotion circulate homogenization under 80MPa pressure and obtains fish oil nano-emulsion 4 times；4）Take clam through spit it is husky, shell, freeze, crush
Clam dry powder is obtained afterwards, by solid-liquid ratio 1:8 are dissolved in distilled water and are homogenized, and are centrifuged 25 minutes in 1 DEG C, 1000r/min, in collection
Clearly, then through Hollow Fiber Ultrafiltration column ultrafiltration, ultrafiltration column molecular cut off is 8 × 104, ultrafiltration collection small-molecular-weight ultrafiltrate, in-
20 DEG C of preservations；The chloroform of 1/5 volume and the n-butanol of 1/25 volume are added in ultrafiltrate, is extracted 3 times；Extract is dialysed,
Meretrix meretrix Glycopeptide crude product is obtained after centrifugation, vacuum drying, centrifuging and taking supernatant after crude product is water-soluble, then with 0-1.0mol/L sodium chloride solutions
Gradient elution, obtains Meretrix meretrix Glycopeptide；5）Take freshwater mussel to be smashed to pieces after spitting husky, cleaning, add the fish meat emulsion of 30% weight, add 2 times
Water, adds the salt of dry weight 3%, postcooling when thermophilic digestion 1 is small, freshwater mussel powder is obtained after low temperature drying and crushing；6）Weigh 80 parts
Freshwater mussel powder, 30 parts of fish oil nano-emulsions, 10 parts of Meretrix meretrix Glycopeptides, 30 portions of earthworms, 30 parts of fly maggots, 15 parts of freshwater lobsters, 50 parts of fresh fish, 10
Part Yolk antibody, 5 parts of sodium butyrates, crush after mixing, cross 50 mesh sieves after low temperature drying and crushing, are made on wet granulator
Grain, particle diameter 0.5mm, food phase special feed is tamed and dociled up to swamp eel；It is full of nutrition that swamp eel tames and dociles food phase special feed, while has the enhancing yellow
The effect of eel seedling immunocompetence and anti-stress ability, can greatly enhance seedling resistance, stop that extraneous pathogenic microorganisms is invaded
Attack, feed has strong attractant effect, by increasing capacitance it is possible to increase the food ration of seedling, the nutritional ingredient enriched in feed can be accelerated newly
Old metabolism, stimulates the secretion of mucus, swamp eel seedling safety is spent the tame and docile food phase.
Embodiment 3：
The preparation method that swamp eel tames and dociles food phase special feed includes：Fermentation, fish oil is modified, prepare fish oil nano-emulsion, Meretrix meretrix Glycopeptide
Preparation, boiling freshwater mussel meat, compounding, specifically include following steps：
Fermentation：The aspergillus oryzae seed liquor of inoculation 5% in fermentation tank, when fermented and cultured 36 is small under the conditions of 32 DEG C, 200r/min,
Nutrient solution is filtered to remove, mycelium is collected after being washed with distilled water 4 times, phosphate buffer immersion 30 is added after being filtered dry distilled water
Minute, the vacuum freeze drying under the conditions of -60 DEG C, obtained aspergillus oryzae filament is saved backup in 4 DEG C of refrigerators；It is prepared by fermentation
Aspergillus oryzae filament method is simple and practicable, and mycelia yield is higher, is modified and prepares for further fish oil；
Fish oil is modified：Molar ratio 1 is added in a reservoir:3.0 glyceride type fish oil and ethyl ester type fish oil, is adding the total matter of fish oil
The aspergillus oryzae filament of amount 5%, adds the functional polypeptide of aspergillus oryzae mycelium weight 1.3%, and sealing is placed on 70 DEG C of temperature
On the water bath with thermostatic control shaking table of 160r/min rotating speeds, reaction 20 it is small when after Cord blood obtain Modified Fish Oil；The ammonia of functional polypeptide
Base acid sequence is：SCASRCKSRCRARRCRARRCGGGICKYFGKRCICKCFRC；Functional polypeptide can act on aspergillus oryzae
The reaction of mycelium and ethyl ester type fish oil, promotes the accounting of EPA and DHA in reaction product, improves the nutritive value of product, Gao Han
The EPA and DHA of amount help to improve the lipid structure in swamp eel seedling body, strengthen the constitution of swamp eel seedling；Microbial enzyme method has
There are high catalytic efficiency, high selectivity, the advantages such as energy consumption is low in industrialized production, microbial enzyme method reaction condition is gentle, to n-3
The structure of PUFAs will not damage, and can greatly improve the content of ethyl ester type fish oil EPA and DHA in product, enzymic catalytic reaction
It can be carried out in solvent-free system, so as to avoid huge production equipment, production safety coefficient higher；
Prepare fish oil nano-emulsion：Fish oil and whey protein isolate are weighed respectively, with the phosphate buffer dissolving whey point of pH7.2
From albumen, the whey protein isolate solution of 28g/L is prepared, after adding 100g/L fish oil while stirring, through height at a temperature of 10 DEG C
Fast emulsification pretreatment machine obtains thick lotion in emulsified 120 seconds in 25000r/min speed conditions, and thick lotion is followed under 120MPa pressure
Ring homogenization 5 times fish oil nano-emulsion；Fish oil is remarkably improved fish oil oxidation stability through nano-emulsion embedding, and HIGH PRESSURE TREATMENT has
Beneficial to the stability for improving fish oil nano-emulsion；
The preparation of Meretrix meretrix Glycopeptide：Take clam through spit it is husky, shell, freeze, crushes after acquisition clam dry powder, by solid-liquid ratio 1：10 are dissolved in
In distilled water and it is homogenized, is centrifuged 30 minutes in 4 DEG C, 2000r/min, collect supernatant, then through Hollow Fiber Ultrafiltration column ultrafiltration, ultrafiltration
Column molecular cut off is 8 × 104, small-molecular-weight ultrafiltrate is collected in ultrafiltration, in -40 DEG C of preservations；2/5 volume is added in ultrafiltrate
Chloroform and 1/20 volume n-butanol, extract 4 times；Extract is dialysed, is centrifuged, be dried in vacuo after to obtain Meretrix meretrix Glycopeptide thick
Product, centrifuging and taking supernatant after crude product is water-soluble, then with 0-1.0mol/L sodium chloride solution gradient elutions, obtain Meretrix meretrix Glycopeptide；Gained clam
The purity of glycopeptide is more than 96%, sugar and protein content each 54% and 42%, and Meretrix meretrix Glycopeptide has excellent antitumor activity, suppression
Bacterium antibacterial activity, can effectively strengthen the immunocompetence of close octopus, strengthen close octopus constitution and vigor, improve incubation rate；
Boiling freshwater mussel meat：Take freshwater mussel to be smashed to pieces after spitting husky, cleaning, add the fish meat emulsion of 50% weight, add 3 times of water, add dry
5% salt is weighed, postcooling when thermophilic digestion 2 is small, freshwater mussel powder is obtained after low temperature drying and crushing；Thermophilic digestion can be by freshwater mussel meat, fish
Microorganism, parasite in meat gruel are killed, and prevent from damaging to swamp eel seedling, while thermophilic digestion can increase freshwater mussel meat, fish
The fragrance of meat gruel, increases attractant and palatability；
Compounding：Weigh 100 parts of freshwater mussel powders, 40 parts of fish oil nano-emulsions, 12 parts of Meretrix meretrix Glycopeptides, 50 portions of earthworms, 40 parts of fly maggots, 30 parts it is light
Fire hose shrimp, 60 parts of fresh fish, 15 parts of Yolk antibodies, 8 parts of sodium butyrates, crush after mixing, and 80 mesh are crossed after low temperature drying and crushing
Sieve, is granulated, particle diameter 3.0mm on wet granulator, food phase special feed is tamed and dociled up to swamp eel；Swamp eel tames and dociles the special feed nutrition of food phase
It is abundant, while have the function that to strengthen swamp eel seedling immunocompetence and anti-stress ability, seedling resistance can be greatly enhanced, is hindered
The extraneous pathogenic microorganisms invasion and attack of gear, feed have strong attractant effect, by increasing capacitance it is possible to increase and the food ration of seedling is abundant in feed
Nutritional ingredient can accelerate metabolism, stimulate the secretion of mucus, swamp eel seedling safety is spent the tame and docile food phase.
Routine operation in operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail in embodiment described above, it should be understood that the above is only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.
Sequence table
<110>Lanxi City You Ze feed technologies Services Co., Ltd
<120>Swamp eel tames and dociles the purposes of food phase special feed
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 39
<212> PRT
<213>Artificial synthesized (Mytilus coruscus)
<400> 1
Ser Cys Ala Ser Arg Cys Lys Ser Arg Cys Arg Ala Arg Arg Cys Arg
1 5 10 15
Ala Arg Arg Cys Gly Gly Gly Ile Cys Lys Tyr Phe Gly Lys Arg Cys
20 25 30
Ile Cys Lys Cys Phe Arg Cys
35
Claims (9)
1. swamp eel tames and dociles the purposes of food phase special feed, it is characterised in that：The swamp eel tames and dociles food phase special feed and is tamed and dociled in swamp eel seedling
The purposes of food phase, the implementation of the purposes are：By swamp eel seedling be transferred to plantation have water plant and continue oxygen supply native pond in,
Oxygen supply exhausting-gas hole should leave pool wall 30-50cm, and the food platform being laid at 1 with water plant must be set per 4-6 square metres of area, and food platform is used
Water plant is laid with and forms；Enter and start to tame and docile food after pond is stopped eating 1-2 days, select to tame during the 45-60 minutes turned on light after the sun sets,
At the same time to turn off water pump, tame and docile food first week feeding volume be swamp eel seedling weight 3-4%, by swamp eel tame and docile food phase special feed and
Man-made feeds are by weight 9-2:1, which carries out mixing, feeds, and the accounting of man-made feeds increases successively, tames and dociles food and is changed to one after 3-4 days
Its 2 meal, the standard that feeds are the second meal without surplus bait；The feeding volume for taming and dociling food second week is the 5-8% of swamp eel weight, and swamp eel is tamed and dociled the food phase
Special feed is by weight 2-0 with man-made feeds:1, which carries out mixing, feeds, and the accounting of man-made feeds increases successively, tames and dociles food 2 weeks
It can terminate to tame and docile the food phase.
2. swamp eel according to claim 1 tames and dociles the purposes of food phase special feed, it is characterised in that：It is special that the swamp eel tames and dociles the food phase
Included with the preparation method of feed：Fermentation, fish oil is modified, prepare fish oil nano-emulsion, the preparation of Meretrix meretrix Glycopeptide, boiling freshwater mussel meat, multiple
Match somebody with somebody, the fish oil modification procedure is：Molar ratio 1 is added in a reservoir:The glyceride type fish oil and ethyl ester type fish oil of 2.8-3.0,
The aspergillus oryzae filament of fish oil gross mass 4-5% is being added, the feature for adding aspergillus oryzae mycelium weight 1.0-1.3% is more
Peptide, sealing be placed on the water bath with thermostatic control shaking table of 68-70 DEG C of temperature and 150-160r/min rotating speeds, reaction 18-20 it is small when after it is low
Temperature preserves to obtain Modified Fish Oil.
3. swamp eel according to claim 2 tames and dociles the purposes of food phase special feed, it is characterised in that：The fish oil modification procedure
In the amino acid sequence of functional polypeptide be：SCASRCKSRCRARRCRARRCGGGICKYFGKRCICKCFRC.
4. swamp eel according to claim 2 tames and dociles the purposes of food phase special feed, it is characterised in that：The fermentation step is：
The aspergillus oryzae seed liquor of 3-5% is inoculated with fermentation tank, fermented and cultured 30-36 is small under the conditions of 30-32 DEG C, 180-200r/min
When, nutrient solution is filtered to remove, mycelium is collected after being washed with distilled water 3-4 times, phosphate buffer is added after being filtered dry distilled water
Immersion 20-30 minutes, the vacuum freeze drying under the conditions of -50~-60 DEG C, by obtained aspergillus oryzae filament in 0-4 DEG C of refrigerator
Save backup.
5. swamp eel according to claim 2 tames and dociles the purposes of food phase special feed, it is characterised in that：It is described to prepare fish oil nanometer
Newborn step is：Fish oil and whey protein isolate are weighed respectively, and egg is separated with the phosphate buffer dissolving whey of pH7.0-7.2
In vain, the whey protein isolate solution of 25-28g/L is prepared, after adding 95-100g/L fish oil while stirring, at a temperature of 4-10 DEG C
Thick lotion is obtained in 20000-25000r/min speed conditions for emulsified 100-120 seconds, thick lotion is existed through high-speed shearing emulsion machine
Homogenization 4-5 time is circulated under 80-120MPa pressure and obtains fish oil nano-emulsion.
6. swamp eel according to claim 2 tames and dociles the purposes of food phase special feed, it is characterised in that：The system of the Meretrix meretrix Glycopeptide
It is for step：Take clam through spit it is husky, shell, freeze, crushes after acquisition clam dry powder, by solid-liquid ratio 1:8-10 is dissolved in distilled water
And be homogenized, centrifuged 25-30 minutes in 1-4 DEG C, 1000-2000r/min, collect supernatant, then through Hollow Fiber Ultrafiltration column ultrafiltration, surpass
Filter column molecular cut off is 8 × 104, small-molecular-weight ultrafiltrate is collected in ultrafiltration, in -20 ~ -40 DEG C of preservations；Added in ultrafiltrate
Chloroform and n-butanol, extract 3-4 times；Extract is dialysed, is centrifuged, be dried in vacuo after obtain Meretrix meretrix Glycopeptide crude product, crude product is water-soluble
Centrifuging and taking supernatant afterwards, then with 0-1.0mol/L sodium chloride solution gradient elutions, obtain Meretrix meretrix Glycopeptide.
7. swamp eel according to claim 6 tames and dociles the purposes of food phase special feed, it is characterised in that：The system of the Meretrix meretrix Glycopeptide
The additive amount of chloroform is the 1/5-2/5 of ultrafiltration volume in standby step, and the additive amount of butanol is the 1/25-1/ of the volume of ultrafiltrate
20。
8. swamp eel according to claim 2 tames and dociles the purposes of food phase special feed, it is characterised in that：The boiling freshwater mussel meat step
For：Take freshwater mussel to be smashed to pieces after spitting husky, cleaning, add the fish meat emulsion of 30-50% weight, add 2-3 times of water, add dry weight 3-
Freshwater mussel powder is obtained after 5% salt, postcooling when thermophilic digestion 1-2 is small, low temperature drying and crushing.
9. swamp eel according to claim 2 tames and dociles the purposes of food phase special feed, it is characterised in that：It is described compounding step be：
Weigh 80-100 parts of freshwater mussel powders, 30-40 parts of fish oil nano-emulsions, 10-12 parts of Meretrix meretrix Glycopeptides, 30-50 portions of earthworms, 30-40 parts of fly maggots,
15-30 parts of freshwater lobsters, 50-60 parts of fresh fish, 10-15 parts of Yolk antibodies, 5-8 parts of sodium butyrates, crush, low temperature is done after mixing
50-80 mesh sieves are crossed after dry and crushing, is granulated on wet granulator, particle diameter 0.5-3.0mm, the special feeding of food phase is tamed and dociled up to swamp eel
Material.
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Application publication date: 20180413 |