CN107897170A - A kind of buccal swab DNA preservation solution and preparation method thereof - Google Patents
A kind of buccal swab DNA preservation solution and preparation method thereof Download PDFInfo
- Publication number
- CN107897170A CN107897170A CN201711141509.8A CN201711141509A CN107897170A CN 107897170 A CN107897170 A CN 107897170A CN 201711141509 A CN201711141509 A CN 201711141509A CN 107897170 A CN107897170 A CN 107897170A
- Authority
- CN
- China
- Prior art keywords
- buccal swab
- preservation solution
- dna preservation
- swab dna
- value
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts
Abstract
The invention discloses a kind of buccal swab DNA preservation solution, include the raw material of following w/v：Trishydroxymethylaminomethane, sodium alginate, tripotassium phosphate, zinc gluconate, disodium ethylene diamine tetraacetate, l-cn, valine, natural antiseptic agent, vitamin e, physiological saline, stevioside, kayexalate, sodium dihydrogen phosphate；Its solvent is deionized water.The DNA preservation solution of the present invention is a kind of preservation liquid preserved for buccal swab, it can preferably preserve Stomatocyte；Its is relatively sanitary, is not easy to grow bacterium；And holding time length is 35 times longer than envelope；Preparation method is simple, has preferable social value.
Description
Technical field
The present invention relates to a kind of DNA preservation solution, is specifically a kind of buccal swab DNA preservation solution and preparation method thereof.
Background technology
Stomatocyte sampling step includes：1) buccal swab outer packing is torn, buccal swab is carefully taken out and (pays attention to：Entirely
Hand cannot contact swab portion in sampling process).2) handle is held, swab is put in into left side oral cavity, swab head is fully connect
Touch inside left cheek/left side on mucous membrane at mandible, wiped up and down with the dynamics brushed teeth it is dynamic, meanwhile, rotate swab, allow swab head
Portion comes into full contact with oral mucosa, repeats this action 1 minute.3) with same method on right side cheek portion/right side at mandible
The sampling of second swab is carried out at mucous membrane.4) will speckle with the swabs of mouth desquamated cells in clean ventilation, at least one hour
Until drying, collection tube is reinstalled, completes sampling.But the preservation of buccal swab be generally placed in it is close in the envelope for carried out mark
Envelope, it is not hygienic enough, easily grow bacterium；And the holding time is short.Therefore, the present invention provide a kind of buccal swab DNA preservation solution and
Its preparation method.
The content of the invention
It is an object of the invention to provide a kind of buccal swab DNA preservation solution and preparation method thereof, to solve above-mentioned background
The problem of being proposed in technology.
To achieve the above object, the present invention provides following technical solution：
A kind of buccal swab DNA preservation solution, includes the raw material of following w/v：Trishydroxymethylaminomethane 11-
15g/100mL, sodium alginate 8-10g/100mL, tripotassium phosphate 3-7g/100mL, zinc gluconate 4-8g/100mL, 0.1-
The disodium ethylene diamine tetraacetate 8-10g/100mL of 0.15mol/L, l-cn 3-5g/100mL, valine 0.01-0.1g/
100mL, natural antiseptic agent 0.05-0.1g/100mL, vitamin e0.5-1.5g/100mL, physiological saline 30-50g/100mL, sweet tea
Synanthrin 1-2g/100mL, kayexalate 1-3g/100mL, sodium dihydrogen phosphate 4-8g/100mL；Its solvent is deionization
Water, pH value 6.8-9.
As the further scheme of the present invention：Include the raw material of following w/v：Trishydroxymethylaminomethane 12g/
100mL, sodium alginate 9g/100mL, tripotassium phosphate 5g/100mL, zinc gluconate 6g/100mL, disodium ethylene diamine tetraacetate
9g/100mL, l-cn 4g/100mL, valine 0.03g/100mL, natural antiseptic agent 0.07g/100mL, vitamin
E0.8g/100mL, physiological saline 40g/100mL, stevioside 1.6g/100mL, kayexalate 2.2g/100mL, phosphoric acid
Sodium dihydrogen 5g/100mL.
As the further scheme of the present invention：Using the disodium ethylene diamine tetraacetate of 0.12mol/L.
As the further scheme of the present invention：Its solvent is deionized water, pH value 7.2-8.8.
As the further scheme of the present invention：The pH value is adjusted by hydrochloric acid.
A kind of preparation method of buccal swab DNA preservation solution, takes trishydroxymethylaminomethane, kayexalate, figured silk fabrics
Propylhomoserin, vitamin e, disodium ethylene diamine tetraacetate, natural antiseptic agent, physiological saline mixing, obtain and preserve basal liquid；By alginic acid
Sodium, tripotassium phosphate, zinc gluconate, l-cn, stevioside, sodium dihydrogen phosphate are mixed with the preservation basal liquid, pass through salt
Acid is 6.8-9 come the buccal swab DNA preservation solution pH value adjusted, obtains buccal swab DNA preservation solution.
A kind of buccal swab DNA preservation solution is used to preserve Stomatocyte.
As the further scheme of the present invention：The Stomatocyte is the Stomatocyte in buccal swab.
Compared with prior art, the beneficial effects of the invention are as follows：
The DNA preservation solution of the present invention is a kind of preservation liquid preserved for buccal swab, it can preferably preserve oral cavity
Cell；Its is relatively sanitary, is not easy to grow bacterium；And holding time length is 3-5 times longer than envelope；Preparation method is simple, has preferable
Social value.
Embodiment
The technical solution of this patent is described in more detail with reference to embodiment.
Embodiment 1
A kind of buccal swab DNA preservation solution, includes the raw material of following w/v：Trishydroxymethylaminomethane 11g/
100mL, sodium alginate 8g/100mL, tripotassium phosphate 3g/100mL, the ethylenediamine tetraacetic of zinc gluconate 4g/100mL, 0.1mol/L
Acetic acid disodium 8g/100mL, l-cn 3g/100mL, valine 0.01g/100mL, natural antiseptic agent 0.05g/100mL, dimension
Raw element e0.5g/100mL, physiological saline 30g/100mL, stevioside 1g/100mL, kayexalate 1g/100mL, phosphoric acid
Sodium dihydrogen 4g/100mL；Its solvent is deionized water, pH value 6.8.The pH value is adjusted by hydrochloric acid.
A kind of preparation method of buccal swab DNA preservation solution, takes trishydroxymethylaminomethane, kayexalate, figured silk fabrics
Propylhomoserin, vitamin e, disodium ethylene diamine tetraacetate, natural antiseptic agent, physiological saline mixing, obtain and preserve basal liquid；By alginic acid
Sodium, tripotassium phosphate, zinc gluconate, l-cn, stevioside, sodium dihydrogen phosphate are mixed with the preservation basal liquid, pass through salt
Acid is 6.8 come the buccal swab DNA preservation solution pH value adjusted, obtains buccal swab DNA preservation solution.
A kind of buccal swab DNA preservation solution is used to preserve Stomatocyte.The Stomatocyte is the oral cavity in buccal swab
Cell.
Embodiment 2
A kind of buccal swab DNA preservation solution, includes the raw material of following w/v：Trishydroxymethylaminomethane 15g/
100mL, sodium alginate 10g/100mL, tripotassium phosphate 7g/100mL, the ethylenediamine of zinc gluconate 8g/100mL, 0.15mol/L
Tetraacethyl disodium 10g/100mL, l-cn 5g/100mL, valine 0.1g/100mL, natural antiseptic agent 0.1g/100mL, dimension
Raw element e1.5g/100mL, physiological saline 50g/100mL, stevioside 2g/100mL, kayexalate 3g/100mL, phosphoric acid
Sodium dihydrogen 8g/100mL；Its solvent is deionized water, pH value 9.The pH value is adjusted by hydrochloric acid.
A kind of preparation method of buccal swab DNA preservation solution, takes trishydroxymethylaminomethane, kayexalate, figured silk fabrics
Propylhomoserin, vitamin e, disodium ethylene diamine tetraacetate, natural antiseptic agent, physiological saline mixing, obtain and preserve basal liquid；By alginic acid
Sodium, tripotassium phosphate, zinc gluconate, l-cn, stevioside, sodium dihydrogen phosphate are mixed with the preservation basal liquid, pass through salt
Acid is 9 come the buccal swab DNA preservation solution pH value adjusted, obtains buccal swab DNA preservation solution.
A kind of buccal swab DNA preservation solution is used to preserve Stomatocyte.The Stomatocyte is the oral cavity in buccal swab
Cell.
Embodiment 3
A kind of buccal swab DNA preservation solution, includes the raw material of following w/v：Trishydroxymethylaminomethane 12g/
100mL, sodium alginate 9g/100mL, tripotassium phosphate 5g/100mL, the ethylenediamine of zinc gluconate 6g/100mL, 0.12mol/L
Tetraacethyl disodium 9g/100mL, l-cn 4g/100mL, valine 0.03g/100mL, natural antiseptic agent 0.07g/100mL,
Vitamin e0.8g/100mL, physiological saline 40g/100mL, stevioside 1.6g/100mL, kayexalate 2.2g/
100mL, sodium dihydrogen phosphate 5g/100mL.Its solvent is deionized water, pH value 7.2.
A kind of preparation method of buccal swab DNA preservation solution, takes trishydroxymethylaminomethane, kayexalate, figured silk fabrics
Propylhomoserin, vitamin e, disodium ethylene diamine tetraacetate, natural antiseptic agent, physiological saline mixing, obtain and preserve basal liquid；By alginic acid
Sodium, tripotassium phosphate, zinc gluconate, l-cn, stevioside, sodium dihydrogen phosphate are mixed with the preservation basal liquid, pass through salt
Acid is 7.2 come the buccal swab DNA preservation solution pH value adjusted, obtains buccal swab DNA preservation solution.
A kind of buccal swab DNA preservation solution is used to preserve Stomatocyte.The Stomatocyte is the oral cavity in buccal swab
Cell.
Embodiment 4
A kind of buccal swab DNA preservation solution, includes the raw material of following w/v：Trishydroxymethylaminomethane 12g/
100mL, sodium alginate 9.5g/100mL, tripotassium phosphate 4.2g/100mL, zinc gluconate 7.6g/100mL, 0.11mol/L's
Disodium ethylene diamine tetraacetate 9.2g/100mL, l-cn 3.5g/100mL, valine 0.09g/100mL, natural antiseptic agent
0.06g/100mL, vitamin e1.3g/100mL, physiological saline 32g/100mL, stevioside 1.7g/100mL, polystyrolsulfon acid
Sodium 1.3g/100mL, sodium dihydrogen phosphate 7g/100mL；Its solvent is deionized water, pH value 8.8.The pH value by hydrochloric acid come
Adjust.
A kind of preparation method of buccal swab DNA preservation solution, takes trishydroxymethylaminomethane, kayexalate, figured silk fabrics
Propylhomoserin, vitamin e, disodium ethylene diamine tetraacetate, natural antiseptic agent, physiological saline mixing, obtain and preserve basal liquid；By alginic acid
Sodium, tripotassium phosphate, zinc gluconate, l-cn, stevioside, sodium dihydrogen phosphate are mixed with the preservation basal liquid, pass through salt
Acid is 8.8 come the buccal swab DNA preservation solution pH value adjusted, obtains buccal swab DNA preservation solution.
A kind of buccal swab DNA preservation solution is used to preserve Stomatocyte.The Stomatocyte is the oral cavity in buccal swab
Cell.
Embodiment 5
A kind of buccal swab DNA preservation solution, includes the raw material of following w/v：Trishydroxymethylaminomethane 14g/
100mL, sodium alginate 8.5g/100mL, tripotassium phosphate 6.3g/100mL, zinc gluconate 4.5g/100mL, 0.13mol/L's
Disodium ethylene diamine tetraacetate 8.8g/100mL, l-cn 4.3g/100mL, valine 0.02g/100mL, natural antiseptic agent
0.08g/100mL, vitamin e0.6g/100mL, physiological saline 48g/100mL, stevioside 1.2g/100mL, polystyrolsulfon acid
Sodium 2.6g/100mL, sodium dihydrogen phosphate 4.5g/100mL；Its solvent is deionized water, pH value 7.5.The pH value passes through hydrochloric acid
To adjust.
A kind of preparation method of buccal swab DNA preservation solution, takes trishydroxymethylaminomethane, kayexalate, figured silk fabrics
Propylhomoserin, vitamin e, disodium ethylene diamine tetraacetate, natural antiseptic agent, physiological saline mixing, obtain and preserve basal liquid；By alginic acid
Sodium, tripotassium phosphate, zinc gluconate, l-cn, stevioside, sodium dihydrogen phosphate are mixed with the preservation basal liquid, pass through salt
Acid is 7.5 come the buccal swab DNA preservation solution pH value adjusted, obtains buccal swab DNA preservation solution.
A kind of buccal swab DNA preservation solution is used to preserve Stomatocyte.The Stomatocyte is the oral cavity in buccal swab
Cell.
Embodiment 6
A kind of buccal swab DNA preservation solution, includes the raw material of following w/v：Trishydroxymethylaminomethane 13.6g/
100mL, sodium alginate 9.2g/100mL, tripotassium phosphate 5.7g/100mL, zinc gluconate 5.8g/100mL, 0.12mol/L's
Disodium ethylene diamine tetraacetate 9.2g/100mL, l-cn 4.2g/100mL, valine 0.06g/100mL, natural antiseptic agent
0.07g/100mL, vitamin e1.1g/100mL, physiological saline 42g/100mL, stevioside 1.4g/100mL, polystyrolsulfon acid
Sodium 2.3g/100mL, sodium dihydrogen phosphate 6.5g/100mL；Its solvent is deionized water, pH value 9.4.The pH value passes through hydrochloric acid
To adjust.
A kind of preparation method of buccal swab DNA preservation solution, takes trishydroxymethylaminomethane, kayexalate, figured silk fabrics
Propylhomoserin, vitamin e, disodium ethylene diamine tetraacetate, natural antiseptic agent, physiological saline mixing, obtain and preserve basal liquid；By alginic acid
Sodium, tripotassium phosphate, zinc gluconate, l-cn, stevioside, sodium dihydrogen phosphate are mixed with the preservation basal liquid, pass through salt
Acid is 9.4 come the buccal swab DNA preservation solution pH value adjusted, obtains buccal swab DNA preservation solution.
A kind of buccal swab DNA preservation solution is used to preserve Stomatocyte.The Stomatocyte is the oral cavity in buccal swab
Cell.
The DNA preservation solution of the present invention is a kind of preservation liquid preserved for buccal swab, it can preferably preserve oral cavity
Cell；Its is relatively sanitary, is not easy to grow bacterium；And holding time length is 3-5 times longer than envelope；Preparation method is simple, has preferable
Social value.
The better embodiment of this patent is explained in detail above, but this patent is not limited to above-mentioned embodiment party
Formula, can also be on the premise of this patent objective not be departed from the knowledge that one skilled in the relevant art possesses
Make a variety of changes.
Claims (7)
1. a kind of buccal swab DNA preservation solution, it is characterised in that include the raw material of following w/v：Trihydroxy methyl amino
Methane 11-15g/100mL, sodium alginate 8-10g/100mL, tripotassium phosphate 3-7g/100mL, zinc gluconate 4-8g/100mL,
The disodium ethylene diamine tetraacetate 8-10g/100mL of 0.1-0.15mol/L, l-cn 3-5g/100mL, valine 0.01-
0.1g/100mL, natural antiseptic agent 0.05-0.1g/100mL, vitamin e 0.5-1.5g/100mL, physiological saline 30-50g/
100mL, stevioside 1-2g/100mL, kayexalate 1-3g/100mL, sodium dihydrogen phosphate 4-8g/100mL；Its solvent is
Deionized water, pH value 6.8-9.
2. buccal swab DNA preservation solution according to claim 1, it is characterised in that include the original of following w/v
Material：Trishydroxymethylaminomethane 12g/100mL, sodium alginate 9g/100mL, tripotassium phosphate 5g/100mL, zinc gluconate 6g/
100mL, disodium ethylene diamine tetraacetate 9g/100mL, l-cn 4g/100mL, valine 0.03g/100mL, natural antiseptic agent
0.07g/100mL, vitamin e 0.8g/100mL, physiological saline 40g/100mL, stevioside 1.6g/100mL, polystyrene sulphur
Sour sodium 2.2g/100mL, sodium dihydrogen phosphate 5g/100mL.
3. buccal swab DNA preservation solution according to claim 1, it is characterised in that using the ethylenediamine tetraacetic of 0.12mol/L
Acetic acid disodium.
4. buccal swab DNA preservation solution according to claim 1, it is characterised in that its solvent is deionized water, and pH value is
7.2-8.8。
5. buccal swab DNA preservation solution according to claim 1, it is characterised in that the pH value is adjusted by hydrochloric acid.
6. a kind of preparation method of buccal swab DNA preservation solution as claimed in claim 5, it is characterised in that take trihydroxy methyl
Aminomethane, kayexalate, valine, vitamin e, disodium ethylene diamine tetraacetate, natural antiseptic agent, physiological saline mix
Close, obtain and preserve basal liquid；By sodium alginate, tripotassium phosphate, zinc gluconate, l-cn, stevioside, sodium dihydrogen phosphate with
The preservation basal liquid mixing, come the buccal swab DNA preservation solution pH value adjusted be 6.8-9 by hydrochloric acid, acquisition buccal swab
DNA preservation solution.
7. buccal swab DNA preservation solution according to claim 5 is used to preserve Stomatocyte, it is characterised in that the mouth
Chamber cell is the Stomatocyte in buccal swab.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711141509.8A CN107897170A (en) | 2017-11-17 | 2017-11-17 | A kind of buccal swab DNA preservation solution and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711141509.8A CN107897170A (en) | 2017-11-17 | 2017-11-17 | A kind of buccal swab DNA preservation solution and preparation method thereof |
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| CN107897170A true CN107897170A (en) | 2018-04-13 |
Family
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| CN201711141509.8A Pending CN107897170A (en) | 2017-11-17 | 2017-11-17 | A kind of buccal swab DNA preservation solution and preparation method thereof |
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Cited By (3)
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|---|---|---|---|---|
| CN111183973A (en) * | 2020-01-20 | 2020-05-22 | 德诺杰亿（北京）生物科技有限公司 | Saliva or oral swab preserving fluid and preparation method and application thereof |
| CN111226912A (en) * | 2020-03-26 | 2020-06-05 | 深圳市天大生物医疗器械有限公司 | Cell preservation solution and preparation method thereof |
| CN113462741A (en) * | 2021-05-16 | 2021-10-01 | 苏州标点生物科技有限公司 | Reagent and method for long-term preservation of nucleic acid at normal temperature |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111183973A (en) * | 2020-01-20 | 2020-05-22 | 德诺杰亿（北京）生物科技有限公司 | Saliva or oral swab preserving fluid and preparation method and application thereof |
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| CN113462741A (en) * | 2021-05-16 | 2021-10-01 | 苏州标点生物科技有限公司 | Reagent and method for long-term preservation of nucleic acid at normal temperature |
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