CN107896987A - A kind of mating system of the wild stone precipice tea tissue-cultured seedling in Guangxi - Google Patents

A kind of mating system of the wild stone precipice tea tissue-cultured seedling in Guangxi Download PDF

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CN107896987A
CN107896987A CN201710959368.4A CN201710959368A CN107896987A CN 107896987 A CN107896987 A CN 107896987A CN 201710959368 A CN201710959368 A CN 201710959368A CN 107896987 A CN107896987 A CN 107896987A
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culture
stone precipice
precipice tea
tissue culture
wild
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CN107896987B (en
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梁干君
钟春城
李秋玲
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Guangxi Jinxiu Yao Autonomous County Junlin Tea Industry Co ltd
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Yao Autonomous County Of Jinxiu Guangxi Zhuang Autonomous Region Research Institute Of Scientific-Technical Information
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants

Abstract

The invention discloses a kind of tissue culture mating system of the wild stone precipice tea in Guangxi, include the following steps：Picking, disinfection, callus tissue culture, the culture of stone precipice tea aseptic seedling bud and the stone precipice tea aseptic seedling culture of rootage of explant, the wild stone precipice tea tissue-cultured seedling mating system of the present invention is easy to operate, survival rate height, the merit that wild stone precipice tea can be kept, it is suitable for the breeding of industrialized tissue culture seedling, for promoting this precious species important in inhibiting of wild stone precipice tea and immeasurable commercial value.

Description

A kind of mating system of the wild stone precipice tea tissue-cultured seedling in Guangxi
Technical field
The invention belongs to crop to breed field, and in particular to a kind of mating system of the wild stone precipice tea tissue-cultured seedling in Guangxi.
Background technology
Stone precipice tea, the yellow auspicious wood of the bright leaf of scientific name, Yang Tong belong to, arbor.It is main to be grown on Guangxi Zhuang Autonomous Region Jinxiuyaozu certainly Mountain Areas of Southern in county is controlled, likes sunshine is short, the temperature difference is big, cool place is moist Stone Mountain cliff or mountain stream side, and because the tea is longer than steep cliff Precipice, is the anima for inhaling the world, the elite for adopting life；Advantageous ecological environment, is pregnant with the pure natural of unique style Drink, ancient times tames and dociles monkey picking, among the people to be also known as " monkey plucks tea ".Detected through relevant department, since stone precipice tea is free of caffeine, after drink Do not influence to sleep, and there is preferable health-care effect rich in Flavonoid substances, therefore be loved by consumers.In the tea Guangdong of stone precipice The plant expert appraisal of mountain university：The wild stone precipice tea in Pingyue County belongs to yellow auspicious wooden Theaceae for Adinandra nitida, and main component is：Flavonoids 20%, Tea polyphenols 23.4%, amino acid 9.73%, and wild alkaloids etc. multiple element beneficial to human body, are had now been found that in natural plant The highest plant of Flavone content in thing, belongs to treasure in the tea of domestic rare ecosystem fully natural green plant.It is in addition, wild The dark brown dark green such as emerald of stone precipice tea, fragrance is tasty and refreshing, and amount containing theophylline is low.Enjoy endless aftertastes after product, do not influence to sleep, it is long-term to drink sooner or later With with clearing heat and detoxicating, liver protection and eyesight, anti-inflammatory moistening lung, beauty treatment, blood pressure lowering, reduce the effect such as fat, stomach strengthening and digestion promoting, to throat Inflammation, obesity, diabetes, hypertension, hyperlipidemia have obvious effect, be Dayao Mountain mountain in treasure.
It is grown on that the wild stone precipice tea breeding survival rate in Jin Xiu counties is extremely low, and the breeding for wild stone precipice tea also becomes stone precipice The hot spot of tea researcher research, we retrieve the mating system data on stone precipice tea as follows：
1. Chinese patent：Patent publication No CN102487706A discloses a kind of stone precipice tea method for culturing seedlings, and this method is every The stone precipice tea fruit of harvesting ripe is real before and after the Frost's Descent in year, using dry Tibetan method and sand storage method storage processing seed, then in the March of next year Bred to during April.This mating system is complex, and is generative propagation, and offspring easily morphs, and is unfavorable for The original characteristic of stone precipice tea is kept, it is higher to environmental requirement, it is impossible to meet the market demand.
2. Chinese patent：Patent authorization number CN103202165B, title：The method of stone precipice tea cuttage and seedling culture, summary：This hair Bright to disclose a kind of method of stone precipice tea cuttage and seedling culture, it is selection moral character fine individual plant, takes annual spray middle and upper part, is cut into Special cuttings branch, bubble medicine promoting root growth, steep nutrient solution, establish seedbed, prepare special overburden soil, cuttage, covering etc..The present invention breaches Stone precipice tea cutting breeding survival rate is extremely low or is unable to the technical barrier of cutting propagation at all, from branch selection, branch trimming, branch Bar processing, slotting machine matrix prepare and are managed and protected etc. after inserting five aspects and start with, and make that stone precipice tea survival rate is high, goes out that garden is fast, outplanting rate is high. Cuttage technique reproductive efficiency is relatively low, it is necessary to which manual manipulative techniques' quantity, maturation, could improve breeding survival rate, be suitable for peasant household Oneself operation, is not suitable for factory or large area breeding.
The content of the invention
The present invention provides a kind of mating system of the wild stone precipice tea tissue-cultured seedling in Guangxi, relative to traditional mating system, has Can keep the merit gene of wild stone precipice tea to greatest extent, easy to operate, survival rate is high, suitable for high-volume or batch production Nursery, to the great social value of breeding and commercial value of wild this rareness species of stone precipice tea.
The technical proposal of the invention is realized in this way：
A kind of tissue culture mating system of the wild stone precipice tea in Guangxi, includes the following steps：
（1）The picking of explant：In 5 annual ~ June, more than 800 meters of height above sea level is selected, is grown on overhanging cliff, nothing throughout the year The wild stone precipice tea that pest and disease damage infects, picks its tender shoots.
（2）Disinfection：After tender shoots is rinsed 3 ~ 4 times repeatedly using pure water, after sterilizing 10 ~ 20min with mercuric chloride solution, with disappearing The filter paper for crossing poison blots, spare.
（3）Callus tissue culture：Proceeded as follows on superclean bench：With the scalpel sterilized it is crosscutting go out length The bud section of 1.2 ~ 1.5cm, bud section is put into callus tissue culture base and is cultivated, in 28 ~ 30 DEG C of temperature, humid control 60 ~ 85%, Light culture 15 ~ 18 days, obtains callus.
（4）The culture of stone precipice tea aseptic seedling bud：The callus without pollution is chosen, it is enterprising to be transferred to subculture multiplication medium Row lures bud culture, cultivates 30 ~ 35 days；Condition of culture：26 ± 2 DEG C of temperature, 2000 ~ 2500lux of intensity of illumination；Treat constantly there is axillary bud Terminate during generation.
（5）Stone precipice tea aseptic seedling culture of rootage：Choose sprout grow 1.5 ~ 2.5cm, subculture sprout, be transferred to root media Upper culture, condition of culture：28 ~ 30 DEG C of temperature, 1200 ~ 1500lux of intensity of illumination；After culture 25 ~ 30 days, master root is trained when obvious Support and terminate.
Step（3）The formula of the callus tissue culture base is MS+N6 0.3 ~ 0.5mg/L+ of benzyladenine methyl α-naphthyl acetates 0.8 2.0 mg/L+ sucrose 30g/L+ agar 4.0g/L, pH 6.8 ~ 7.2 of ~ 1.5mg/L+ vitamin Cs.
Step（4）The squamous subculture based formulas is MS+ sucrose 4.0g/L+N6 ~ 0.3 ~ 0.5mg/L of benzyl purine+naphthalene 0.3 ~ 0.8mg/L of acetic acid+agar 5g/L, pH 6.0 ~ 6.5.
Step（5）The formula of the root media is 3 ~ 6g/L of MS+ sucrose+0.5 ~ 1.4mg/L of heteroauxin+naphthalene second Acid 0.5 ~ 1.3mg/L+IBA 0.1mg/L+ manganese sulfate 0.5 ~ 1.5g/L+, 6 ~ 8g/L of agar, pH6.0 ~ 6.4.
Beneficial effects of the present invention are：
The wild stone precipice tea in Guangxi Gold show county is grown on overhanging cliff throughout the year, is that natural environment was changed by 10000000 year, the survival of the fittest The rareness species to survive, due to lacking research to its biological characteristics and growth rhythm and condition, people by grafting, The mating system of cuttage, it is difficult to success.The present invention, will by the investigation and the research of gene to wild stone precipice tea biological characteristics Its growth is healthy and strong, maintains the tender shoots of wild stone precipice tea merit as explantation tissue's culture, by induce axillary bud growth, Culture of rootage, has cultivated the wild stone precipice tea seedling that survival rate is high, late growth can keep merit.The present invention's is wild Stone precipice tea tissue-cultured seedling mating system is easy to operate, is suitable for the breeding of industrialized tissue culture seedling, for promote wild stone precipice tea this Precious species important in inhibiting and immeasurable commercial value.
Embodiment
Embodiment 1
A kind of tissue culture mating system of the wild stone precipice tea in Guangxi, includes the following steps：
（1）The picking of explant：In 5 annual ~ June, more than 800 meters of height above sea level is selected, is grown on overhanging cliff, nothing throughout the year The wild stone precipice tea that pest and disease damage infects, picks its tender shoots.
（2）Disinfection：After tender shoots is rinsed 3 ~ 4 times repeatedly using pure water, after sterilizing 10 ~ 20min with mercuric chloride solution, with disappearing The filter paper for crossing poison blots, spare.
（3）Callus tissue culture：Proceeded as follows on superclean bench：With the scalpel sterilized it is crosscutting go out length The bud section of 1.2 ~ 1.5cm, bud section is put into callus tissue culture base and is cultivated, in 28 ~ 30 DEG C of temperature, humid control 60 ~ 85%, Light culture 15 days, obtains callus；The formula of callus tissue culture base is MS+N6 benzyladenine 0.3mg/L+ methyl α-naphthyl acetates 2.0 mg/L+ sucrose 30g/L+ agar 4.0g/L, pH 6.8 of 0.8mg/L+ vitamin Cs.
（4）The culture of stone precipice tea aseptic seedling bud：The callus without pollution is chosen, it is enterprising to be transferred to subculture multiplication medium Row lures bud culture, cultivates 30 days；Condition of culture：26 ± 2 DEG C of temperature, 2000 ~ 2500lux of intensity of illumination；Treat constantly there is axillary bud life Into when terminate；Squamous subculture based formulas is MS+ sucrose 4.0g/L+N6 ~ benzyl purine 0.3mg/L+methyl α-naphthyl acetate 0.3mg/L+fine jade Fat 5g/L, pH 6.0.
（5）Stone precipice tea aseptic seedling culture of rootage：Choose sprout grow 1.5 ~ 2.5cm, subculture sprout, be transferred to root media Upper culture, condition of culture：28 ~ 30 DEG C of temperature, 1200 ~ 1500lux of intensity of illumination；After culture 25 days, master root is cultivated when obvious Terminate；The formula of root media is MS+ sucrose 3g/L+heteroauxin 0.5mg/L+methyl α-naphthyl acetate 0.5mg/L+IBA 0.1mg/L+ manganese sulfate 0.5g/L+ agar 6g/L, pH6.0.
By the mating system of above-mentioned tissue-cultured seedling, wild stone precipice tea tissue culture shoot survival percent 92.5%, after tissue-cultured seedling culture Transplanting afforestation, transplanting survival rate 86.78%, successfully transplanting is afforested 157 mu at present.
Embodiment 2
A kind of tissue culture mating system of the wild stone precipice tea in Guangxi, includes the following steps：
（1）The picking of explant：In 5 annual ~ June, more than 800 meters of height above sea level is selected, is grown on overhanging cliff, nothing throughout the year The wild stone precipice tea that pest and disease damage infects, picks its tender shoots.
（2）Disinfection：After tender shoots is rinsed 3 ~ 4 times repeatedly using pure water, after sterilizing 10 ~ 20min with mercuric chloride solution, with disappearing The filter paper for crossing poison blots, spare.
（3）Callus tissue culture：Proceeded as follows on superclean bench：With the scalpel sterilized it is crosscutting go out length The bud section of 1.2 ~ 1.5cm, bud section is put into callus tissue culture base and is cultivated, in 28 ~ 30 DEG C of temperature, humid control 60 ~ 85%, Light culture 18 days, obtains callus；The formula of callus tissue culture base is MS+N6 benzyladenine 0.5mg/L+ methyl α-naphthyl acetates 1.5mg/L+ vitamin Cs 2.0 mg/L+ sucrose 30g/L+ agar 4.0g/L, pH7.2.
（4）The culture of stone precipice tea aseptic seedling bud：The callus without pollution is chosen, it is enterprising to be transferred to subculture multiplication medium Row lures bud culture, cultivates 35 days；Condition of culture：26 ± 2 DEG C of temperature, 2000 ~ 2500lux of intensity of illumination；Treat constantly there is axillary bud life Into when terminate；Squamous subculture based formulas is MS+ sucrose 4.0g/L+N6 ~ benzyl purine 0.5mg/L+methyl α-naphthyl acetate 0.8mg/L+fine jade Fat 5g/L, pH 6.5.
（5）Stone precipice tea aseptic seedling culture of rootage：Choose sprout grow 1.5 ~ 2.5cm, subculture sprout, be transferred to root media Upper culture, condition of culture：28 ~ 30 DEG C of temperature, 1200 ~ 1500lux of intensity of illumination；After culture 30 days, master root is cultivated when obvious Terminate；The formula of root media is MS+ sucrose 6g/L+heteroauxin 1.4mg/L+methyl α-naphthyl acetate 1.3mg/L+IBA 0.1mg/L+ manganese sulfate 1.5g/L+ agar 6 ~ 8g/L, pH6.4.
Using the present embodiment method, wild stone precipice tea tissue culture shoot survival percent 91.2%, transplants after tissue-cultured seedling culture and makes Woods, transplanting survival rate 83.4%, successfully transplanting is afforested 225 mu at present.
Embodiment 3
A kind of tissue culture mating system of the wild stone precipice tea in Guangxi, includes the following steps：
（1）The picking of explant：In 5 annual ~ June, more than 800 meters of height above sea level is selected, is grown on overhanging cliff, nothing throughout the year The wild stone precipice tea that pest and disease damage infects, picks its tender shoots.
（2）Disinfection：After tender shoots is rinsed 3 ~ 4 times repeatedly using pure water, after sterilizing 10 ~ 20min with mercuric chloride solution, with disappearing The filter paper for crossing poison blots, spare.
（3）Callus tissue culture：Proceeded as follows on superclean bench：With the scalpel sterilized it is crosscutting go out length The bud section of 1.2 ~ 1.5cm, bud section is put into callus tissue culture base and is cultivated, in 28 ~ 30 DEG C of temperature, humid control 60 ~ 85%, Light culture 168 days, obtains callus；The formula of callus tissue culture base is MS+N6 benzyladenine 0.4mg/L+ methyl α-naphthyl acetates 2.0 mg/L+ sucrose 30g/L+ agar 4.0g/L, pH 6.9 of 0.8mg/L+ vitamin Cs.
（4）The culture of stone precipice tea aseptic seedling bud：The callus without pollution is chosen, it is enterprising to be transferred to subculture multiplication medium Row lures bud culture, cultivates 32 days；Condition of culture：26 ± 2 DEG C of temperature, 2000 ~ 2500lux of intensity of illumination；Treat constantly there is axillary bud life Into when terminate；Squamous subculture based formulas is MS+ sucrose 4.0g/L+N6 ~ benzyl purine 0.3mg/L+methyl α-naphthyl acetate 0.5mg/L+fine jade Fat 5g/L, pH 6.3.
（5）Stone precipice tea aseptic seedling culture of rootage：Choose sprout grow 1.5 ~ 2.5cm, subculture sprout, be transferred to root media Upper culture, condition of culture：28 ~ 30 DEG C of temperature, 1200 ~ 1500lux of intensity of illumination；After culture 26 days, master root is cultivated when obvious Terminate；The formula of root media is MS+ sucrose 5g/L+heteroauxin 1.0mg/L+methyl α-naphthyl acetate 0.8mg/L+IBA 0.1mg/L+ manganese sulfate 0.8g/L+ agar 6 ~ 8g/L, pH6.2.
Using the present embodiment method, wild stone precipice tea tissue culture shoot survival percent is up to 95.5%, this batch of tissue-cultured seedling not yet goes out garden and make Woods.
Embodiment 4
A kind of tissue culture mating system of the wild stone precipice tea in Guangxi, includes the following steps：
（1）The picking of explant：In 5 annual ~ June, more than 800 meters of height above sea level is selected, is grown on overhanging cliff, nothing throughout the year The wild stone precipice tea that pest and disease damage infects, picks its tender shoots.
（2）Disinfection：After tender shoots is rinsed 3 ~ 4 times repeatedly using pure water, after sterilizing 10 ~ 20min with mercuric chloride solution, with disappearing The filter paper for crossing poison blots, spare.
（3）Callus tissue culture：Proceeded as follows on superclean bench：With the scalpel sterilized it is crosscutting go out length The bud section of 1.2 ~ 1.5cm, bud section is put into callus tissue culture base and is cultivated, in 28 ~ 30 DEG C of temperature, humid control 60 ~ 85%, Light culture 15 days, obtains callus；The formula of callus tissue culture base is MS+N6 benzyladenine 0.5mg/L+ methyl α-naphthyl acetates 1.5mg/L+ vitamin Cs 2.0 mg/L+ sucrose 30g/L+ agar 4.0g/L, pH7.0.
（4）The culture of stone precipice tea aseptic seedling bud：The callus without pollution is chosen, it is enterprising to be transferred to subculture multiplication medium Row lures bud culture, cultivates 35 days；Condition of culture：26 ± 2 DEG C of temperature, 2000 ~ 2500lux of intensity of illumination；Treat constantly there is axillary bud life Into when terminate；Squamous subculture based formulas is MS+ sucrose 4.0g/L+N6 ~ benzyl purine 0.3mg/L+methyl α-naphthyl acetate 0.3mg/L+fine jade Fat 5g/L, pH 6.2.
（5）Stone precipice tea aseptic seedling culture of rootage：Choose sprout grow 1.5 ~ 2.5cm, subculture sprout, be transferred to root media Upper culture, condition of culture：28 ~ 30 DEG C of temperature, 1200 ~ 1500lux of intensity of illumination；After culture 28 days, master root is cultivated when obvious Terminate；The formula of root media is MS+ sucrose 6g/L+heteroauxin 1.0mg/L+methyl α-naphthyl acetate 1.0mg/L+IBA 0.1mg/L+ manganese sulfate 0.8g/L+ agar 6 ~ 8g/L, pH6.2.
Using the present embodiment method, wild stone precipice tea tissue culture shoot survival percent 96.1%, transplants after tissue-cultured seedling culture and makes Woods, transplanting survival rate 80.2%, successfully transplanting is afforested 55 mu at present.
Embodiment 5
A kind of tissue culture mating system of the wild stone precipice tea in Guangxi, includes the following steps：
（1）The picking of explant：In 5 annual ~ June, more than 800 meters of height above sea level is selected, is grown on overhanging cliff, nothing throughout the year The wild stone precipice tea that pest and disease damage infects, picks its tender shoots.
（2）Disinfection：After tender shoots is rinsed 3 ~ 4 times repeatedly using pure water, after sterilizing 10 ~ 20min with mercuric chloride solution, with disappearing The filter paper for crossing poison blots, spare.
（3）Callus tissue culture：Proceeded as follows on superclean bench：With the scalpel sterilized it is crosscutting go out length The bud section of 1.2 ~ 1.5cm, bud section is put into callus tissue culture base and is cultivated, in 28 ~ 30 DEG C of temperature, humid control 60 ~ 85%, Light culture 18 days, obtains callus；The formula of callus tissue culture base is MS+N6 benzyladenine 0.3mg/L+ methyl α-naphthyl acetates 2.0 mg/L+ sucrose 30g/L+ agar 4.0g/L, pH 6.8 of 0.8mg/L+ vitamin Cs.
（4）The culture of stone precipice tea aseptic seedling bud：The callus without pollution is chosen, it is enterprising to be transferred to subculture multiplication medium Row lures bud culture, cultivates 35 days；Condition of culture：26 ± 2 DEG C of temperature, 2000 ~ 2500lux of intensity of illumination；Treat constantly there is axillary bud life Into when terminate；Squamous subculture based formulas is MS+ sucrose 4.0g/L+N6 ~ benzyl purine 0.5mg/L+methyl α-naphthyl acetate 0.4mg/L+fine jade Fat 5g/L, pH6.5.
（5）Stone precipice tea aseptic seedling culture of rootage：Choose sprout grow 1.5 ~ 2.5cm, subculture sprout, be transferred to root media Upper culture, condition of culture：28 ~ 30 DEG C of temperature, 1200 ~ 1500lux of intensity of illumination；After culture 26 days, master root is cultivated when obvious Terminate；The formula of root media is MS+ sucrose 6g/L+heteroauxin 0.5mg/L+methyl α-naphthyl acetate 0.5mg/L+IBA 0.1mg/L+ manganese sulfate 0.5g/L+ agar 6 ~ 8g/L, pH6.3.
Using the present embodiment method, wild stone precipice tea tissue culture shoot survival percent 94.0%, transplants after tissue-cultured seedling culture and makes Woods, transplanting survival rate 86.25%, successfully transplanting is afforested 332 mu at present.

Claims (4)

  1. A kind of 1. tissue culture mating system of the wild stone precipice tea in Guangxi, it is characterised in that：Include the following steps：
    （1）The picking of explant：In 5 annual ~ June, more than 800 meters of height above sea level is selected, is grown on overhanging cliff, nothing throughout the year The wild stone precipice tea that pest and disease damage infects, picks its tender shoots；
    （2）Disinfection：After tender shoots is rinsed 3 ~ 4 times repeatedly using pure water, after sterilizing 10 ~ 20min with mercuric chloride solution, use is sterilized Filter paper blot, it is spare；
    （3）Callus tissue culture：Proceeded as follows on superclean bench：With the scalpel sterilized it is crosscutting go out it is long by 1.2 ~ The bud section of 1.5cm, bud section is put into callus tissue culture base and is cultivated, in 28 ~ 30 DEG C of temperature, humid control 60 ~ 85%, dark training Support 15 ~ 18 days, obtain callus；
    （4）The culture of stone precipice tea aseptic seedling bud：The callus without pollution is chosen, is transferred in subculture multiplication medium and is lured Bud culture, is cultivated 30 ~ 35 days；Condition of culture：26 ± 2 DEG C of temperature, 2000 ~ 2500lux of intensity of illumination；Treat constantly there is axillary buds formation When terminate；
    （5）Stone precipice tea aseptic seedling culture of rootage：The subculture sprout that sprout grows 1.5 ~ 2.5cm is chosen, is transferred on root media and trains Support, condition of culture：28 ~ 30 DEG C of temperature, 1200 ~ 1500lux of intensity of illumination；After culture 25 ~ 30 days, knot is cultivated when master root is obvious Beam.
  2. 2. the tissue culture mating system of the wild stone precipice tea in Guangxi according to claim 1, it is characterised in that：Step（3）It is described The formula of callus tissue culture base is MS+N6 benzyladenine 0.3 ~ 0.5mg/L+ methyl α-naphthyl acetate 0.8 ~ 1.5mg/L+ vitamin Cs 2.0 mg/L+ sucrose 30g/L+ agar 4.0g/L, pH 6.8 ~ 7.2.
  3. 3. the tissue culture mating system of the wild stone precipice tea in Guangxi according to claim 1, it is characterised in that：Step（4）It is described Squamous subculture based formulas is MS+ sucrose 4.0g/L+N6 ~ 0.3 ~ 0.5mg/L of benzyl purine+0.3 ~ 0.8mg/L of methyl α-naphthyl acetate+fine jade Fat 5g/L, pH 6.0 ~ 6.5.
  4. 4. the tissue culture mating system of the wild stone precipice tea in Guangxi according to claim 1, it is characterised in that：Step（5）It is described The formula of root media is 3 ~ 6g/L of MS+ sucrose+0.5 ~ 1.4mg/L of heteroauxin+0.5 ~ 1.3mg/L of methyl α-naphthyl acetate+IBA 0.1mg/L+ manganese sulfate 0.5 ~ 1.5g/L+, 6 ~ 8g/L of agar, pH6.0 ~ 6.4.
CN201710959368.4A 2017-10-16 2017-10-16 Breeding method of Guangxi wild adinandra nitida tissue culture seedlings Active CN107896987B (en)

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Publication number Priority date Publication date Assignee Title
CN108541592A (en) * 2018-05-18 2018-09-18 象州县科学技术情报研究所 The tissue culture mating system of kapok

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CN106106003A (en) * 2016-08-18 2016-11-16 广西壮族自治区桂林茶叶科学研究所 A kind of mating system of YESHENG CHA nursery stock
CN106818474A (en) * 2017-01-18 2017-06-13 福建农林大学 The tissue culture method of tea tree rataria is cultivated using micro-organisms base

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CN102007867A (en) * 2009-09-07 2011-04-13 湖南省林业科学院 Efficient rooting method for oil tea clone tissue culture seedlings
CN101822160A (en) * 2010-05-05 2010-09-08 广西壮族自治区林业科学研究院 Method using tissue-subculture sprouts of oil-tea camellia to raise seedlings by grafting
CN102422810A (en) * 2011-09-02 2012-04-25 安徽农业大学 In-vitro regeneration culture method for tea clones
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108541592A (en) * 2018-05-18 2018-09-18 象州县科学技术情报研究所 The tissue culture mating system of kapok

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