CN107875192A - A kind of method of polyphenol substance in supercritical extract walnut kernel pellicle - Google Patents
A kind of method of polyphenol substance in supercritical extract walnut kernel pellicle Download PDFInfo
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- CN107875192A CN107875192A CN201711101837.5A CN201711101837A CN107875192A CN 107875192 A CN107875192 A CN 107875192A CN 201711101837 A CN201711101837 A CN 201711101837A CN 107875192 A CN107875192 A CN 107875192A
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- walnut kernel
- supercritical
- extract
- polyphenol substance
- extraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/52—Juglandaceae (Walnut family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
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- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of method of polyphenol substance in supercritical extract walnut kernel pellicle, and it mainly will first be cleaned with kind of skin walnut kernel, dries, after crushing, then successively by enzymolysis, homogeneous, centrifugation and supercritical CO2Extraction, moisture most is removed through vacuum freeze drying afterwards, obtains polyphenol extract；Compared with prior art; the polyphenol substance simple and convenient extraction of the present invention; the polyphenol substance content of extraction is high; by being digested to walnut kernel, homogenization; effectively separate out the polyphenol substance of walnut kernel so that dispersion micronized, homogenization, supercritical extraction technique are ripe in walnut pulp; extraction efficiency is high, effectively increases the collection of polyphenol substance.
Description
Technical field
The present invention relates to a kind of method of polyphenol substance in supercritical extract walnut kernel pellicle, belong to oils deep process technology
Field.
Background technology
Supercritical extract has the characteristics of rectifying and liquid-solid extraction concurrently, using pressure and temperature to supercritical fluid solvability
Influence and carry out the separation of material；Supercritical carbon dioxide fluid has the advantages that safe noresidue, is surpassed using most
Critical fluids, it is widely used in the preparation or refining of walnut kernel.
Walnut kernel rich in the nutrition such as grease, protein and vitamin, and with stomach invigorating, enrich blood, moistening lung, kidney-nourishing and benefit
The multiple efficacies such as brain, it is a kind of good invigorant, it is nutritious and delicious, therefore have " long live ", " Longevity ", " intelligence development again
Fruit ", the good reputation of " Beauty Fruits ".Modern study shows the multiple work(such as anti-oxidant, the anti-aging and prevention of cardiovascular disease of walnut kernel
Effect is mainly attributed to grease, protein and the polyphenols enriched in walnut kernel, at present, to polyphenol substance in walnut kernel pellicle
Extracting method it is a lot, but can run into mostly cost height, complex process, extraction polyphenol content it is relatively low and extraction
The problems such as condition is restricted high.
The content of the invention
In order to alleviate shortcomings and deficiencies of the prior art, it is an object of the invention to provide a kind of supercritical extract walnut kernel
The method of polyphenol substance in kind skin.
Technical scheme is as follows：
The method of polyphenol substance, specifically includes herein below in supercritical extract walnut kernel pellicle：
(1) fresh band kind skin walnut kernel is taken, microwave drying after cleaning, is then crushed, crosses 100-200 mesh sieves, institute
The power for the microwave drying stated is 200-220W, drying time 5-10min；
(2) according to walnut meat powder：Water quality ratio is 1:4-5 ratio adds water into walnut meat powder and carries out moisture regulation,
0.2%-0.5% complex enzyme hydrolysis enzymes are added after stirring and evenly mixing thereto, described complex enzyme hydrolysis enzyme is by pectase and cellulase
According to 1：2-3 ratios form, and adjust Ph to 8.0-8.5, and warming-in-water is to 45-50 DEG C, enzymolysis time 1.5-2.5h；
(3) walnut kernel after ferment treatment is starched into homogenization, temperature is 70-80 DEG C, using double-stage homogenization, one-level homogeneous pressure
Power is 20-25Mpa, double-stage homogenization pressure 6-10Mpa, and extracting centrifugal liquid is centrifuged after homogeneous；
(4) centrifugate of step (3) is sent into supercritical CO2Extracted in device, described supercritical CO2Device
Pressure is 10-12MPa, and extraction temperature is 30-35 DEG C, extraction time 25-35min, collects extract.
(5) above-mentioned steps (4) extract is removed into moisture through vacuum freeze drying, obtains polyphenol extract.
Described complex enzyme hydrolysis enzyme is by pectase and cellulase according to 1：2.5 ratios form.
Water-bath is warming up to 48 DEG C in described enzymolysis process, enzymolysis time 2.0h.
Described supercritical CO2The pressure of device is 10MPa, and extraction temperature is 32 DEG C, extraction time 30min.
Beneficial effects of the present invention：
Compared with prior art, polyphenol substance simple and convenient extraction of the invention, the polyphenol substance content of extraction is high, passes through
Walnut kernel is digested, homogenization, effectively separate out the polyphenol substance of walnut kernel so that dispersion micronized in walnut pulp,
Homogenization, supercritical extraction technique is ripe, and extraction efficiency is high, effectively increases the collection of polyphenol substance.
Embodiment
Embodiment 1：The method of polyphenol substance, specifically includes herein below in supercritical extract walnut kernel pellicle：
(1) fresh band kind skin walnut kernel is taken, microwave drying after cleaning, is then crushed, crosses 100 mesh sieves, it is described
The power of microwave drying is 200W, drying time 8min；
(2) according to walnut meat powder：Water quality ratio is 1:4.5 ratio adds water into walnut meat powder and carries out moisture regulation,
0.35% complex enzyme hydrolysis enzyme is added after stirring and evenly mixing thereto, described complex enzyme hydrolysis enzyme is by pectase and cellulase according to 1：
2.5 ratios form, and adjust Ph to 8.0, and warming-in-water is to 48 DEG C, enzymolysis time 2.0h；
(3) walnut kernel after ferment treatment is starched into homogenization, temperature is 75 DEG C, using double-stage homogenization, one-level homogenization pressure
For 22Mpa, double-stage homogenization pressure 8Mpa, extracting centrifugal liquid is centrifuged after homogeneous；
(4) centrifugate of step (3) is sent into supercritical CO2Extracted in device, described supercritical CO2Device
Pressure is 10MPa, and extraction temperature is 32 DEG C, extraction time 30min, collects extract.
(5) above-mentioned steps (4) extract is removed into moisture through vacuum freeze drying, obtains polyphenol extract.
Claims (4)
1. a kind of method of polyphenol substance in supercritical extract walnut kernel pellicle, it is characterised in that specifically include herein below：
(1) fresh band kind skin walnut kernel is taken, microwave drying after cleaning, is then crushed, crosses 100-200 mesh sieves, it is described
The power of microwave drying is 200-220W, drying time 5-10min；
(2) according to walnut meat powder：Water quality ratio is 1:4-5 ratio adds water into walnut meat powder and carries out moisture regulation, stirring
Add 0.2%-0.5% complex enzyme hydrolysis enzymes after mixing thereto, described complex enzyme hydrolysis enzyme by pectase and cellulase according to
1：2-3 ratios form, and adjust Ph to 8.0-8.5, and warming-in-water is to 45-50 DEG C, enzymolysis time 1.5-2.5h；
(3) walnut kernel after ferment treatment is starched into homogenization, temperature is 70-80 DEG C, and using double-stage homogenization, one-level homogenization pressure is
20-25Mpa, double-stage homogenization pressure 6-10Mpa, extracting centrifugal liquid is centrifuged after homogeneous；
(4) centrifugate of step (3) is sent into supercritical CO2Extracted in device, described supercritical CO2The pressure of device
For 10-12MPa, extraction temperature is 30-35 DEG C, extraction time 25-35min, collects extract.
(5) above-mentioned steps (4) extract is removed into moisture through vacuum freeze drying, obtains polyphenol extract.
2. the method for polyphenol substance in supercritical extract walnut kernel pellicle according to claim 1, it is characterised in that described
Complex enzyme hydrolysis enzyme by pectase and cellulase according to 1：2.5 ratios form.
3. the method for polyphenol substance in supercritical extract walnut kernel pellicle according to claim 1, it is characterised in that described
Enzymolysis process in water-bath be warming up to 48 DEG C, enzymolysis time 2.0h.
4. the method for polyphenol substance in supercritical extract walnut kernel pellicle according to claim 1, it is characterised in that described
Supercritical CO2The pressure of device is 10MPa, and extraction temperature is 32 DEG C, extraction time 30min.
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| CN201711101837.5A CN107875192A (en) | 2017-11-10 | 2017-11-10 | A kind of method of polyphenol substance in supercritical extract walnut kernel pellicle |
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| CN201711101837.5A CN107875192A (en) | 2017-11-10 | 2017-11-10 | A kind of method of polyphenol substance in supercritical extract walnut kernel pellicle |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114601860A (en) * | 2022-04-16 | 2022-06-10 | 吉林大学 | Preparation method of polyphenol in mung bean hull |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106805180A (en) * | 2017-01-13 | 2017-06-09 | 江南大学 | A kind of method that polyphenol substance is extracted in desmoenzyme and the ultrasonically treated endotesta walnut kernel by band |
-
2017
- 2017-11-10 CN CN201711101837.5A patent/CN107875192A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106805180A (en) * | 2017-01-13 | 2017-06-09 | 江南大学 | A kind of method that polyphenol substance is extracted in desmoenzyme and the ultrasonically treated endotesta walnut kernel by band |
Non-Patent Citations (1)
| Title |
|---|
| 郜海燕等: "山核桃多酚物质提取及抗氧化研究进展", 《食品科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114601860A (en) * | 2022-04-16 | 2022-06-10 | 吉林大学 | Preparation method of polyphenol in mung bean hull |
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Application publication date: 20180406 |