CN107875057A - Cosmetic composition and its preparation method and application - Google Patents
Cosmetic composition and its preparation method and application Download PDFInfo
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- CN107875057A CN107875057A CN201711234939.4A CN201711234939A CN107875057A CN 107875057 A CN107875057 A CN 107875057A CN 201711234939 A CN201711234939 A CN 201711234939A CN 107875057 A CN107875057 A CN 107875057A
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- ginseng
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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Abstract
The invention discloses a kind of cosmetic composition and preparation method thereof.It is 100 that the cosmetic composition, which includes weight ratio,:0.2~5 flower of Panax ginseng distillate and extract of ginseng flower；Wherein, the preparation method of extract of ginseng flower includes：Flower of Panax ginseng is extracted with water, aqueous extract concentration, it is 60~80vol% to add ethanol water to the concentration of alcohol that concentration is more than 90vol%, filtration, is extracted after filtrate recycling ethanol with n-butanol, obtains n-butanol extracting liquid；N-butanol extracting liquid is reclaimed into n-butanol, dries, obtains extract of ginseng flower.The cosmetic composition of the present invention has significant whitening spot-removing and/or anti-aging effects.
Description
Technical field
The present invention relates to a kind of cosmetic composition, the cosmetics particularly with whitening spot-removing and/or anti-aging effects
Composition, further relate to the preparation method and application of said composition.
Background technology
Ginseng is that traditional Chinese medicine, its beauty functions are increasingly favored by people simply.It is generally believed that contain in ginseng
The composition with whitening effect be Ginsenosides, be added into a variety of cosmetics.
At present, when flower of Panax ginseng is applied in cosmetics, extracting method is mostly individually or uses water after being mixed with other medicinal materials
After extraction or ethanol water extraction, without refined or through being further refining to obtain extract.For example, Application No.
200910192274.4 Chinese patent application discloses a kind of whitening and spot eliminating cream, and active component therein is Chinese medicine extract,
Chinese medicine is made up of the component of following percetage by weight：Hand ginseng 5~55%, flower of Panax ginseng 15~35%, Flos Albiziae 5~15%, gold and silver
Spend 5~15%, rose 15~20%, chrysanthemum 5~10%；The preparation method of the Chinese medicine extract is：By each Chinese medicine in proportion
Mixing, is soaked, and is heated to boiling and is kept constant temperature, filters out decoction；The dregs of a decoction separately add water, are again heated to boiling slow fire and decoct,
Decoction is filtered out, after merging decoction twice, is condensed into traditional Chinese medicine extraction concentrate；The traditional Chinese medicine extraction concentrate is applied in face cream.
The Chinese patent application of Application No. 201310372317.3 discloses a kind of oral Chinese medicine composition, and it is by ginseng
Flower, honeysuckle, FLOS CHRYSANTHEMI ALBA from Haizhou of China and radix glycyrrhizae are made, for adjusting endocrine, skin maintenance nti-freckle.In the Chinese medicine composition, flower of Panax ginseng
It is prepared using the method for water extraction and upper large pore resin absorption column.
The content of the invention
Present inventor's discovery, the distillate and the water extract-alcohol precipitation of flower of Panax ginseng that flower of Panax ginseng progress steam distillation is obtained
The mixture that extract again through n-butanol extraction forms has very significant whitening spot-removing and/or anti-aging effects.Herein
On the basis of complete the present invention.Specifically, the present invention includes herein below.
It is an object of the present invention to provide a kind of cosmetic composition, and it has significant whitening spot-removing and/or resisted
Aging acts on.
Another object of the present invention is to provide the preparation method of cosmetic composition, it can be prepared with whitening
The cosmetic composition of nti-freckle and/or anti-aging effects, and preparation method is simple.
It is still another object of the present invention to provide the cosmetics containing the cosmetic composition.
A further object of the present invention is the application for providing described cosmetic composition.
In the present invention, described flower of Panax ginseng refers to Araliaceae (Araliaceae) plant ginseng (Panax Ginseng
C.A.Meyer drying flower or dry flower).
The purpose of the present invention is achieved by the following technical solution：
The present invention provides a kind of cosmetic composition, and it includes flower of Panax ginseng distillate and extract of ginseng flower, and the people
Ginseng flower distillate and the weight ratio of extract of ginseng flower are 100:0.2~5, preferable weight ratio is 100:0.5~2；Wherein, institute
The preparation method for stating extract of ginseng flower comprises the following steps：Flower of Panax ginseng is obtained with water extraction and arrives aqueous extract, by the water extraction
It is 60~80vol% to take liquid to add ethanol water to the concentration of alcohol that concentration is more than 90vol% after concentrating, and is filtered
Liquid, the filtrate are extracted after removing ethanol with n-butanol, obtain n-butanol extracting liquid, and the n-butanol extracting liquid removes positive fourth
Alcohol, dry, obtain the extract of ginseng flower.
According to an embodiment of the invention, the cosmetic composition is only by above-mentioned flower of Panax ginseng distillate and ginseng
Flower extract forms.
In the present invention, the flower of Panax ginseng distillate refers to the distillate for obtaining flower of Panax ginseng progress steam distillation.It is preferred that
Ground, the flower of Panax ginseng distillate includes or the only saturation of flowers volatile oil containing ginseng distillate.Ginseng flowers volatile oil saturation distillate is
Refer to, after carrying out steam distillation to flower of Panax ginseng, the volatile oil content in distillate is saturation., can be to distillate in the present invention
Liquid upper strata forms index of the oil phase as volatile oil saturation in distillate.The flower of Panax ginseng distillate can also contain above-mentioned oil
Phase.
The present invention also provides the preparation method of above-mentioned cosmetic composition.
The preparation method of the cosmetic composition of the present invention includes mixing the flower of Panax ginseng distillate and extract of ginseng flower
The step of conjunction.
According to one preferred embodiment of the invention, the preparation method comprises the following steps：
(1) flower of Panax ginseng is mixed with water, heating carries out steam distillation, respectively obtains flower of Panax ginseng aqueous extract and flower of Panax ginseng
Distillate；
(2) the flower of Panax ginseng aqueous extract is concentrated, it is dense to ethanol adds the ethanol water that concentration is more than 90vol%
Spend and obtain filtrate for 60~80vol%, filtration, filtrate is extracted after removing ethanol with n-butanol, obtains n-butanol extracting liquid；It is described
N-butanol extracting liquid removes n-butanol, dries, obtains extract of ginseng flower；
(3) it is 100 according to weight ratio by the flower of Panax ginseng distillate and extract of ginseng flower:0.2~5 ratio mixing,
Obtain the cosmetic composition.
Preparation in accordance with the present invention, it is preferable that in step (1), in order to which the active ingredient in flower of Panax ginseng is fully carried
Take out, the flower of Panax ginseng can be the coarse powder of the mesh of particle diameter 20~50.Flower of Panax ginseng medicinal material can be entered by using conventional method
Row crushes, the method for sieving obtains the coarse powder.
Preparation in accordance with the present invention, it is preferable that in step (1), the flower of Panax ginseng distillate is preferably as described above
Volatile oil saturation flower of Panax ginseng distillate.Oil phase can be formed using distillate upper strata as the finger of volatile oil saturation in distillate
Mark.In the present invention, the flower of Panax ginseng distillate can include the oil phase, can also not include the oil phase and only include water
Phase.Inventor has found, when the flower of Panax ginseng distillate does not include the oil phase, and only includes aqueous phase for preparing cosmetics spy
It is not that to prepare mild cosmetics highly beneficial, and adverse side effect will not be produced.Its reason is unclear, and inventor speculates may
Reason is in some compositions in oil phase to skin irritant.Conventional method can be used by removing the method for oil phase, such as be adopted
Removed with separatory funnel.
Preparation in accordance with the present invention, it is preferable that in step (1), the weight of water is 8~25 times of flower of Panax ginseng weight,
More preferably 10~20 times, be further preferably 12~18 times.According to the preferred embodiment of the present invention, the weight of water is behaved
12~16 times of ginseng flower weight.Amount of water is very little, it is impossible to which guarantee obtains the flower of Panax ginseng distillate of sufficient amount；Amount of water is excessive, then
Cause the waste of water and the energy.
Preparation in accordance with the present invention, it is preferable that in step (1), the flower of Panax ginseng distillate is to connect since distillation
Continuous collect, weight is 3~12 times of flower of Panax ginseng weight, more preferably 3~10 times, further preferably the distillate for 4~8 times.This
Inventor has found that above-mentioned distillate has excellent whitening spot-removing and/or anti-aging effects, and does not contain other unfavorable compositions；Together
When efficiency high and will not cause to waste when accessing within the above range.
In the present invention, it is preferable that the flower of Panax ginseng distillate is the distillate continuously collected, but not including that being opened from distillation
Beginning continuously collects, weight is 0.05~0.5 times of amount of flower of Panax ginseng weight, is preferably 0.1~0.3 times of distillate, and collect
The weight of flower of Panax ginseng distillate is 3~10 times, more preferably 4~8 times of flower of Panax ginseng weight.Using such processing mode, obtain
The distillate arrived with flower of Panax ginseng water extract when combining with more excellent whitening spot-removing and/or anti-aging effects.
Preparation in accordance with the present invention, it is preferable that in step (2), the flower of Panax ginseng aqueous extract is concentrated, adds ethanol
It is 65~75vol%, more preferably 68~72vol% to concentration of alcohol.
Preparation in accordance with the present invention, it is preferable that in step (3), the flower of Panax ginseng distillate and extract of ginseng flower
Weight ratio be 100:0.5~2.
Preparation in accordance with the present invention, the flower of Panax ginseng distillate is without concentration.
The present invention also provides a kind of cosmetics, and above-mentioned cosmetic composition is contained in the cosmetics.The cosmetics can
Think mildy wash；Toner；Gel；Distillate medicinal water；Aromatic waters；Face, eye, hand, the cream agent of foot, mellite, breast
Liquor, Essence, facial mask liquid, film etc..It can also contain other whitening spot-removings and/or anti-aging class in the cosmetics of the present invention
Active component and/or auxiliary material.The auxiliary material include but is not limited to vaseline, lanolin, carbomer, atoleine, polyethylene glycol,
Propane diols, glycerine, hyaluronic acid etc..
The present invention also provides described cosmetic composition and is used to prepare the change with whitening spot-removing and/or anti-aging effects
The application of cosmetic.
The main component of most of cosmetics is water.The cosmetic composition of the present invention is aqueous phase, and it, which is used to prepare, makes up
Can directly it be used during product, without the extra addition for adding water or water being reduced.Such as when for preparing toner,
Other active components and/or auxiliary material can be directly added into the cosmetic composition of the present invention, without extra addition water, or only
Additionally add a small amount of water.
The active component of the cosmetic composition of the present invention is the mixture of extract of ginseng flower and flower of Panax ginseng distillate.Should
Composition uses through zoopery and volunteer, has very significant whitening spot-removing effect；Also have in addition obvious anti-ageing
Old effect.Cosmetic composition obtained by currently preferred preparation method, property is gentle, nonirritant, suitable for preparing temperature
With type cosmetics.Currently preferred flower of Panax ginseng preparation method has widened the application site of flower of Panax ginseng, realizes the abundant of resource
Utilize.
Embodiment
With reference to specific embodiment, the present invention is further illustrated, but protection scope of the present invention is not limited to
This.
Preparation example 1
Ginseng pollen is broken into the coarse powder that particle diameter is 24 mesh, mixed with water, amount of water is 12 times of flower of Panax ginseng weight, is heated
Steam distillation is carried out, accesses to 0.1 times of amount of flower of Panax ginseng weight, discards when distillate flows out；Continue to access to flower of Panax ginseng
The distillate of 6 times of amounts of weight, removes the oil phase on top, that is, obtains flower of Panax ginseng distillate.
Preparation example 2
Ginseng pollen is broken into the coarse powder that particle diameter is 24 mesh, mixed with water, amount of water is 16 times of flower of Panax ginseng weight, is heated
Steam distillation is carried out, starts to access distillate when distillate flows out, to distillating for 6 times of amounts for accessing flower of Panax ginseng weight
Liquid, obtain flower of Panax ginseng distillate.
Preparation example 3
Ginseng pollen is broken into the coarse powder that particle diameter is 24 mesh, water is added as solvent heating and refluxing extraction 2 times, adds water every time
Measure as 8 times of flower of Panax ginseng weight, each extraction time is followed successively by 1 hour, 0.5 hour, obtains flower of Panax ginseng aqueous extract；By the people
Ginseng flower aqueous extract is concentrated into every milliliter of equivalent to 0.5 gram flower of Panax ginseng medicinal material, adds 95vol% ethanol water to concentration of alcohol
For 70vol%, filter, extracted after filtrate recycling ethanol with n-butanol, obtain n-butanol extracting liquid；N-butanol extracting liquid is reclaimed
N-butanol, dry, obtain extract of ginseng flower.
Embodiment 1
By the extract of ginseng flower of the flower of Panax ginseng distillate of preparation example 1 and preparation example 3 using weight ratio as 100:0.5 proportioning
It is well mixed, obtain cosmetic composition.
Embodiment 2
By the extract of ginseng flower of the flower of Panax ginseng distillate of preparation example 1 and preparation example 3 using weight ratio as 100:1 proportioning is mixed
Close uniformly, obtain cosmetic composition.
Embodiment 3
By the extract of ginseng flower of the flower of Panax ginseng distillate of preparation example 1 and preparation example 3 using weight ratio as 100:2 proportioning is mixed
Close uniformly, obtain cosmetic composition.
Embodiment 4
Ginseng pollen is broken into the coarse powder that particle diameter is 24 mesh, mixed with water, amount of water is 12 times of flower of Panax ginseng weight, is heated
Steam distillation is carried out, accesses to 0.1 times of amount of flower of Panax ginseng weight, discards when distillate flows out；Continue to access to flower of Panax ginseng
The distillate of 6 times of amounts of weight, removes the oil phase on top, that is, obtains flower of Panax ginseng distillate.
The remaining flower of Panax ginseng aqueous extract of steam distillation is concentrated into every milliliter of equivalent to 0.5 gram flower of Panax ginseng medicinal material, adds
95vol% ethanol water to concentration of alcohol is 70vol%, filtering, is extracted, obtained just with n-butanol after filtrate recycling ethanol
Butanol extract solution；N-butanol extracting liquid is reclaimed into n-butanol, dries, obtains extract of ginseng flower.
According to weight ratio it is 100 by the flower of Panax ginseng distillate and extract of ginseng flower:2 is well mixed, obtains cosmetics
Composition.
Embodiment 5
Take 1wt% Sodium Hyaluronates 5g, glycerine 5g, 10wt% collagen 5g, emulsifying agent G57 1g, SIMMONDSIA CHINENSIS SEED OIL 6g,
Vitamin E 1g, saualane 1g, pentanediol 1g, hexylene glycol 1g, the cosmetic composition 74g that embodiment 3 obtains, stir,
Emulsion is made.
Embodiment 6
4wt% carbomer solution 100g is taken, is slowly added to triethanolamine 5g, side edged stirs, until being formed uniform solidifying
Glue, adds glycerine 20g, 1wt% hyaluronic acid 25g, pentanediol 5g, hexylene glycol 2.5g in the case of stirring, and embodiment 4 obtains
Cosmetic composition 210g, stir, gel be made.
Embodiment 7
The cosmetic composition 480g that embodiment 1 is obtained, it is equal to add 1wt% hyaluronic acids 10g, hexylene glycol 10g mixing
It is even, facial mask water is made.
Embodiment 8
The cosmetics group that 1wt% Sodium Hyaluronates 16g, glycerine 10g, pentanediol 4g, hexylene glycol 2g, embodiment 4 are obtained
Compound 130g is well mixed, and is heated to 75 DEG C, standby as aqueous phase；Separately take plant emulsifying wax 12g, coconut oil 30g, vitamin E
2g, saualane 24g, are heated to 75 DEG C, are added slowly with stirring in aqueous phase, stirring while adding, and face cream is made.
Comparative example 1
Add water 127.4g to dissolve the extract of ginseng flower 2.6g of preparation example 3, obtain extract of ginseng flower solution；It will implement
" cosmetic composition that embodiment 4 obtains " replaces with the extract of ginseng flower solution in example 8, and other are constant, obtain this contrast
The face cream of example.
Comparative example 2
" cosmetic composition that embodiment 4 obtains " in embodiment 8 is replaced with to the flower of Panax ginseng distillate of preparation example 1, its
He is constant, obtains the face cream of this comparative example.
Experimental example 1
Pass through the whitening spot-removing and function of anti-skin aging of the cosmetic composition of the test of pesticide effectiveness checking present invention.Experiment side
Method and result are as follows：
1. animal and material
Kunming mouse, SPF levels are female, 20 ± 2g of body weight, in the Fourth Military Medical University of P.L.A experimental animal
The heart provides, credit number:SCXK (army) 2012-0007.
Catalase (CAT) determines kit lot number：20170301.
SOD kits, lot number：20161115.
MDA kits, lot number：20161118.
Hydroxyproline (HYP) determines kit, lot number：20161118.
Above kit is purchased from Nanjing and builds up Bioengineering Research Institute.
D- galactolipins, lot number：20161111, Shanghai Pu Zhen bio tech ltd.
D- galactose solutions：D- galactolipins are dissolved in 0.9% physiological saline, are made into volumetric concentration 200mg/ml, standby.
2. experimental method
2.1 experimental animal modeling and processing method
50 mouse are taken to be randomly divided into blank group, model group, experimental group, 1 group of control, 2 groups, every group 10 of control.Blank
Group injection 200mg/kg physiological saline, remaining four groups equal nape parts are subcutaneously injected 200mg/kg D- galactose solutions, established
D- galactolipins cause mice aging model.
While modeling, every 2 days shavings once, the skin part of shaving is administered each group mouse.Blank group and mould
Type group gives distilled water 0.2ml/10g daily, is coated on 4cm2On the skin of area.Experimental group gives the face of embodiment 8 daily
White 0.2g/10g, is coated on 4cm2On the skin of area.Compare 1 group and comparative example 1 and comparative example are given in 2 groups of control daily respectively
2 face cream 0.2g/10g, is coated on 4cm2On the skin of area.Continuous 42d.
2.2HYP, CAT, SOD, MDA Indexs measure
Last dose cervical dislocation execution mouse, back unhairing after 2 hours, the skin histology two at coating position is taken rapidly
Block, one of normal saline flushing with ice, blood is removed, filter paper blots, with 1:9 (W/V) add ice physiological saline, Yu Bing
The homogenate of 10% tissue is made in bath, is centrifuged 15 minutes with 3500r/min rotating speeds, takes supernatant, and skin is detected by the method for kit
SOD, MDA, CAT and HYP content.
The PATHOMORPHOLOGICAL OBSERVATION OF PULLORUM of 2.3 dermal melanin cells
The skin at another piece of coating position is taken, is fixed with 10% neutral formalin, routine paraffin wax embedded section, melanin mark
Using immunohistochemical method, primary antibody selects mouse anti-human monoclonal's antibody HMB 45, DAB colour developings, and haematoxylin is redyed.Use PBS
Negative control is done instead of primary antibody.To observe distribution situation and quantity of the melanocyte in epidermis.
3. experimental result
Experimental result is shown in Table 1.
Each index content of the mouse skin of table 1
Compared with model group, * represents that P ＜ 0.05, * * represent P ＜ 0.01.
The influence of 3.1 pairs of mouse skin SOD, MDA, CAT and HYP contents
As shown in table 1, experimental group model mice skin SOD of the present invention, CAT vigor significantly raise (P ＜ 0.05), and model is small
Mouse Content of MDA significantly reduces (P ＜ 0.01), and HYP content significantly raises in mouse skin.
Superoxide dismutase activity is improved in experimental group model mouse skin of the present invention, and oxygen freedom is removed so as to strengthen body
The ability of base；The generation of MDA is reduced, and has an obvious oxidation resistant ability, and oxidation resistance is better than 1 group of control and right
According to 2 groups；HYP content increase (P ＜ 0.01) in experimental group skin, its positive effect is better than 2 groups of (P ＜ of 1 group of control and control
0.05)；So as to effectively remove skin histology free radical, there are significant anti-aging effects.
The influence of 3.2 mouse skin melanocytes
The mark observations of HMB 45：Visible a small amount of melanocyte in normal group hair follicle, sebaceous glands basal cell.Model group is black
Chromatophore cluster is distributed in hair follicle, sebaceous glands basal cell, and distribution is also dispersed in epidermal basal cell and stratum spinosum epidermidis.
Experimental group of the present invention is with compareing 1 group, compareing the melanocyte that only sporadically appeared in 2 groups of basal layer of epidermis and hair follicle basal cell.Such as table 1
Shown, from the point of view of melanocyte number, model group and the obvious increase (P ＜ 0.01) of blank group, experimental group of the present invention is small
Mouse dermal melanin cell quantity substantially reduces (P ＜ 0.01) compared with model group, and melanocyte number significantly lower than control 1 group,
Compare 2 groups.As a result show that the present invention has the function that good reduction model mice dermal melanin cell.
As fully visible, the present invention can improve SOD vigor in model mice skin, reduce MDA contents, and increase containing for HYP
Amount, and the quantity of melanocyte in model mice skin can be significantly reduced, there is good anti-aging, the work of whitening spot-removing
With.
Experimental example 2
Acted on by the anti-light aging of the cosmetic composition of the test of pesticide effectiveness checking present invention.Experimental method and result are such as
Under：
2.1. animal and material
Kunming mouse, SPF levels are female, 20 ± 2g of body weight, in the Fourth Military Medical University of P.L.A experimental animal
The heart provides, credit number:SCXK (army) 2012-0007.
Coomassie brilliant blue protein quantification determines kit lot number：20161118, catalase (CAT) measure kit batch
Number：20170301, SOD kits, lot number：20161115；MDA kits, lot number：20161118, hydroxyproline (HYP) measure
Kit, lot number：20161118；Kit is purchased from Nanjing and builds up Bioengineering Research Institute；D- galactolipins, lot number：
20161111, Shanghai Pu Zhen bio tech ltd.
2.2. experimental method
Take 50 mouse be randomly divided into blank group, model group, experimental group, control 1 group, control 2 groups, every group 10, each group
Mouse back shaving area 2cm × 2cm, except blank control group, other each group mouse are placed under fluorescent ultraviolet lamp (UVA+UVB, mould
Intend daylight) irradiation, irradiation distance 50cm.Irradiation 1h daily, irradiate 5 times weekly, Continuous irradiation 8 weeks, UVB (280-320nm) is tired
Product exposure dose is 21.60Jcm-2。
While modeling each group mouse every 2 days shavings once, the skin part of shaving by setting dosage administration.Normally
Group and model group give distilled water 0.2ml/10g in the corresponding time, are coated on 4cm2On the skin of area.Experimental group is given daily
The face cream 0.2g/10g of embodiment 8, is coated on 4cm2On the skin of area.Compare 1 group and 2 groups of control is given contrast respectively daily
The face cream 0.2g/10g of example 1 and comparative example 2, is coated on 4cm2On the skin of area.Continuous 42d.
Last dose cervical dislocation execution mouse, back unhairing after 2 hours, take rapidly the skin histology at coating position, use
Ice-cold normal saline flushing, blood is removed, filter paper blots, with 1:9 (W/V) add ice physiological saline, and 10% is made in ice bath
Tissue homogenate, with 3500r/min rotating speeds centrifuge 15 minutes, take supernatant, by kit method detect skin SOD, MDA,
CAT, HYP content.
2.3. experimental result
Notable elevation model mouse skin SOD, CAT vigor (P ＜ 0.05) of experimental group of the present invention, significantly reduces model mouse blood
Clear MDA contents (P ＜ 0.01), and the content (P ＜ 0.01) of HYP in mouse skin is significantly improved, it is shown in Table 2.
As a result show that the present invention can improve superoxide dismutase activity in model mouse skin, oxygen is removed so as to strengthen body
The ability of free radical, the generation of MDA is reduced, there is obvious oxidation resistant ability, oxidation resistance is significantly stronger than control 1
2 groups of group and control；Increase the content of HYP in skin, its positive effect is better than 2 groups of 1 group of control and control.The present invention can be effectively
Remove ROS to protect cells from damaging, make to be removed the enhancing of peroxide ability by the skin of ultraviolet irradiation, and can effectively safeguard
The generation of body free radical and the dynamic equilibrium removed.
Table 2 light aging mouse skin SOD, MDA, CAT, HYP measurement result table
Compared with model group, * represents that P ＜ 0.05, * * represent P ＜ 0.01.
To sum up, cosmetic composition of the invention can effectively remove skin histology free radical, have significant anti-light old
The effect of change.
The present invention is not limited to above-mentioned embodiment, in the case of without departing substantially from the substantive content of the present invention, this area skill
Any deformation, improvement, the replacement that art personnel are contemplated that each fall within the scope of the present invention.
Claims (10)
1. a kind of cosmetic composition, it is characterised in that it includes flower of Panax ginseng distillate and extract of ginseng flower, and the ginseng
Flower distillate and the weight ratio of extract of ginseng flower are 100:0.2~5；
Wherein, the preparation method of the extract of ginseng flower comprises the following steps：Flower of Panax ginseng is obtained with water extraction and arrives aqueous extract,
It is 60~80vol% that ethanol water to the concentration of alcohol that concentration is more than 90vol% is added after the aqueous extract is concentrated,
Filtration obtains filtrate, and the filtrate is extracted after removing ethanol with n-butanol, obtains n-butanol extracting liquid, the n-butanol extracting liquid
N-butanol is removed, dries, obtains the extract of ginseng flower.
2. cosmetic composition according to claim 1, it is characterised in that flower of Panax ginseng distillate and extract of ginseng flower
Weight ratio is 100:0.5~2.
3. cosmetic composition according to claim 1 or 2, it is characterised in that ginseng described in the flower of Panax ginseng distillate
Flower distillate includes or the only saturation of flowers volatile oil containing ginseng distillate.
4. the preparation method of the cosmetic composition according to any one of claims 1 to 3, it is characterised in that methods described
The step of including the flower of Panax ginseng distillate and extract of ginseng flower are mixed.
5. preparation method according to claim 4, it is characterised in that methods described comprises the following steps：
(1) flower of Panax ginseng is mixed with water, heating carries out steam distillation, respectively obtains flower of Panax ginseng aqueous extract and flower of Panax ginseng distillation
Liquid；
(2) the flower of Panax ginseng aqueous extract is concentrated, adding ethanol water to the concentration of alcohol that concentration is more than 90vol% is
60~80vol%, filtration obtain filtrate, and the filtrate is extracted after removing ethanol with n-butanol, obtains n-butanol extracting liquid；It is described
N-butanol extracting liquid removes n-butanol, dries, obtains extract of ginseng flower；
(3) it is 100 according to weight ratio by the flower of Panax ginseng distillate and extract of ginseng flower:0.2~5 ratio mixing, is obtained
The cosmetic composition.
6. preparation method according to claim 5, it is characterised in that in step (1), the weight of water is flower of Panax ginseng weight
8~25 times；Flower of Panax ginseng distillate is 3~12 times of distillate continuously collected since distillation, that weight is flower of Panax ginseng weight.
7. preparation method according to claim 5, it is characterised in that the flower of Panax ginseng distillate distillates for what is continuously collected
Thing, but not including that since distillation start continuously collect, weight be flower of Panax ginseng weight 0.05~0.5 times of distillate, and receipts
The weight of the flower of Panax ginseng distillate integrated is 3~10 times of flower of Panax ginseng weight.
8. a kind of cosmetics, it is comprising the cosmetic composition according to any one of claims 1 to 3 or according to claim
Cosmetic composition made from preparation method described in 4~7 any one.
9. cosmetics according to claim 8, it is characterised in that the cosmetics are selected from mildy wash；Toner；Gel
Agent；Distillate medicinal water；Aromatic waters；Face, eye, cream agent, mellite, emulsion agent, facial mask liquid, Essence or the film of hand or foot
Agent.
10. cosmetic composition according to any one of claims 1 to 3 or according to any one of claim 4~7
Cosmetic composition made from preparation method is used for the application for preparing the cosmetics with whitening spot-removing and/or anti-aging effects.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113768836A (en) * | 2021-09-06 | 2021-12-10 | 浙江菊丝明生物科技有限公司 | Compound skin cream containing fibroin and chrysanthemum morifolium extract and preparation method thereof |
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| CN85100318A (en) * | 1985-04-01 | 1986-08-06 | 辽宁省中医研究院 | Extraction process for total saponin from leaf, flower and stem of ginseng |
| KR20100059347A (en) * | 2008-11-26 | 2010-06-04 | (주)아모레퍼시픽 | Composition of skin external application containing ginseng flower essential oil |
| CN103479694A (en) * | 2008-06-13 | 2014-01-01 | 株式会社爱茉莉太平洋 | External preparation composition for skin comprising ginseng flower or ginseng seed extracts |
| CN107213086A (en) * | 2017-06-07 | 2017-09-29 | 安徽吉乃尔电器科技有限公司 | Wrinkle collagen gel facial mask and preparation method thereof is removed in a kind of whitening |
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2017
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN85100318A (en) * | 1985-04-01 | 1986-08-06 | 辽宁省中医研究院 | Extraction process for total saponin from leaf, flower and stem of ginseng |
| CN103479694A (en) * | 2008-06-13 | 2014-01-01 | 株式会社爱茉莉太平洋 | External preparation composition for skin comprising ginseng flower or ginseng seed extracts |
| KR20100059347A (en) * | 2008-11-26 | 2010-06-04 | (주)아모레퍼시픽 | Composition of skin external application containing ginseng flower essential oil |
| CN107213086A (en) * | 2017-06-07 | 2017-09-29 | 安徽吉乃尔电器科技有限公司 | Wrinkle collagen gel facial mask and preparation method thereof is removed in a kind of whitening |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113768836A (en) * | 2021-09-06 | 2021-12-10 | 浙江菊丝明生物科技有限公司 | Compound skin cream containing fibroin and chrysanthemum morifolium extract and preparation method thereof |
| CN113768836B (en) * | 2021-09-06 | 2024-02-02 | 浙江菊丝明生物科技有限公司 | A compound skin lotion containing fibroin and flos Chrysanthemi extract, and its preparation method |
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