CN107873944A - A kind of industrialized production rapeseed active peptide and preparation method - Google Patents
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
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- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
- A23J1/142—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by extracting with organic solvents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention discloses a kind of method of industrialized production rapeseed active peptide.The present invention is digested using rapeseed as raw material using trypsase, papain and neutral proteinase multienzyme, and then by processes such as enzyme deactivation, centrifugation, filtering, concentration, decolouring and spray drying, rapeseed active peptide is made.Component of its molecular weight less than 1000Da accounts for more than 90%, and free aminoacid content is less than 5%, and tripeptides leucine tyrosine proline (Leu Tyr Pro, LYP) sequence peptide fragment at least containing 2.5% content.The production method of the present invention has bioactivity height, processing cost is low, treating capacity is big, is easy to the advantages of industrialized production, the rapeseed active peptide prepared in this approach can be applied to the exploitation and production of ordinary food, health food and medicine as new functional nutrient composition, have wide market prospects.
Description
Technical field
The invention belongs to biological technical field, and in particular to a kind of industrialized production rapeseed active peptide and preparation method.
Background technology
Rapeseed, also referred to as brassicae chinensis,semen, be draft crop in cruciferae, be Chinese main oil crops and nectar source crop it
One, its seed is one of immersion oil fat raw material principal item processed.Cultivation is divided into two kinds of winter rape and spring rape throughout China.Its kind
Plant area and account for more than the 40% of the Chinese oil crops gross area, yield accounts for more than the 30% of Chinese oil plant total output, occupies world head
Position.General nitrogenous 3.9%-5.2% in rapeseed, protein is between 24.6%-32.4%.
By carrying out protease hydrolyzed to canola, the oligopeptide by proteolysis into small-molecular-weight, considerably increase
The dissolubility of product, the success of rapeseed active peptide are prepared as traditional food canola deep processing of farm products and provide one newly
Type industrialization road, this abundant ordinary food protein resource of canola is fully used, expand rape significantly
Seed powder improves the added value of large agricultural product, played bigger as a kind of application field of traditional ordinary food raw material
Economic value, and improve comprehensive social benefit.Compared with former albumen, the rapeseed active peptide of small molecule is more prone to be disappeared
Change and absorb.Due to a series of its good food processing properties, rapeseed active peptide also can be with the protein of other separate sources
Mixing, to improve its nutritive value, it can also be used to produce instant drink, drinks, meat products, dairy products, bakery product etc.
Diversified food.With the development of science and technology, the application of protein engineering, using zymolysis technique produce biologically active peptide and
The research and product development of its protolysate have become focus and main flow, and enzymolysis rapeseed protein produces the experiment of polypeptide
The existing part of room technical literature, but the preparation method for producing rapeseed peptide in industry does not have also.
The content of the invention
Present invention aims at a kind of preparation method of rapeseed active peptide is provided, to solve technical problem.
A kind of preparation method of rapeseed active peptide, comprises the following steps：
A, rapeseed is pre-processed, carries out ultramicro grinding, cross 200-600 mesh, used with 100: 2~10 (L: kg) liquid ratio
Water and canola mixing are sized mixing, and are adjusted the micro- basifications of pH 8-10, are heated to 60~80 DEG C.30~120min of insulated and stirred；
B, the alkaline feed liquid in retort is pumped into centrifuge, is separated into clear liquid and slag charge, collect slag charge, by slag charge plus
Water dilutes, and is heated to 60~80 DEG C, stirs and separate, and is carried out 3 times with same processing mode；
C, the pH value of supernatant is adjusted to after 4-6, mechanical agitation 8-12h, centrifugation, collects precipitation, precipitation as rapeseed
Albumen, it is repeated twice；
D, precipitation plus water are redissolved, is heated to 75-100 DEG C, time 15-45min so that protein denaturation, then carry out
It is high-pressure homogeneous；
E, above-mentioned pH value of solution is adjusted to 5-10, adds special enzyme preparation: trypsase: papain: neutral proteinase
For 5~7: 1~2: 3~5, hydrolysis temperature is 40-65 DEG C, time 1.5-8h；
F, after enzymolysis terminates, 90-125 DEG C of enzyme-removal temperature, the enzyme deactivation time is 1-3h, and thick enzymolysis liquid is obtained after terminating；
G：For the centrifugation of thick enzymolysis liquid, filtering, evaporation and concentration, addition activated carbon decolorizing, then spray drying are obtained into rape
Seed active peptide powder.
The preparation method of rapeseed active peptide described above, it is characterised in that in described step A, carry out ultramicro grinding
Enzymatic hydrolyzation can be improved, makes full use of material composition.
The preparation method of rapeseed active peptide described above, it is characterised in that in described step C, using thermal denaturation
Method, it can be more favorable for digesting, enzymolysis is more abundant.High-pressure homogeneous, further raising enzymolysis is carried out after thermal denaturation simultaneously
Efficiency.
The preparation method of rapeseed active peptide described above, it is characterised in that described step D, special enzyme preparation combination
Including trypsase: papain: neutral proteinase is 5~7: 1~2: 3~5.Research finds the combination of its special enzyme preparation
The enzymolysis efficiency of the long-range about single enzyme of enzymolysis efficiency.
The preparation method of rapeseed active peptide described above, it is characterised in that be spray-dried used for middle cold nebulization
Drying tower, further retain thermal activity material, and middle low-temperature spray drying tower is to be applied first in peptide technique.
A kind of preparation method of rapeseed active peptide, it is characterised in that according to the preparation method described in claim 1, institute
State rapeseed oligopeptide and be characterized in that component of the molecular weight less than 1000Da accounts for more than 90%, free aminoacid content is less than
5%, and the tripeptides leucine at least containing 2.5% content-Tyr-Pro (Leu-Tyr-Pro, LYP) sequence peptide fragment.
In addition to objects, features and advantages described above, the present invention also has other objects, features and advantages.
The present invention is further detailed explanation below.
Embodiment
Embodiments of the invention are described in detail below, but the present invention can be limited and covered according to claim
Multitude of different ways implement.
1st, a kind of preparation method of rapeseed active peptide, comprises the following steps：
A, rapeseed is pre-processed, carries out ultramicro grinding, cross 200-600 mesh, with 100: 7 (L: kg) liquid ratio water and
Canola mixing is sized mixing, and is adjusted 9 micro- basifications of pH, is heated to 70 DEG C.Insulated and stirred 80min；
B, the alkaline feed liquid in retort is pumped into centrifuge, is separated into clear liquid and slag charge, collect slag charge, by slag charge plus
Water dilutes, and is heated to 70 DEG C, stirs and separate, and is carried out 3 times with same processing mode；
C, the pH value of supernatant is adjusted to after 5, precipitation is collected in mechanical agitation 10h, centrifugation, precipitation as rapeseed protein,
It is repeated twice；
D, precipitation plus water are redissolved, is heated to 85 DEG C, time 30min so that protein denaturation, it is equal then to enter horizontal high voltage
Matter；
E, above-mentioned pH value of solution is adjusted to 6, adds special enzyme preparation: trypsase: papain: neutral proteinase is：
4.5: 1.5: 4, trypsase: papain: the addition of neutral proteinase is every gram of unit of protein 1500, hydrolysis temperature
For 50 DEG C, time 3h；
F, after enzymolysis terminates, 90-125 DEG C of enzyme-removal temperature, the enzyme deactivation time is 1-3h, and thick enzymolysis liquid is obtained after terminating；
G：To centrifuge, filtering by thick enzymolysis liquid, filtered using 0.05~0.1 μm of the membrane filter system in aperture, condition
For 0.2~0.4MPa of pressure, 30~80 DEG C of temperature.It is concentrated by evaporation, adds activated carbon decolorizing, adds concentrate total solids content
5% quality of activated carbon is decolourized, and then spray drying obtains rapeseed active peptide powder.
2nd, the physics and chemistry composition of rapeseed active peptide and molecular weight distribution analysis
The composition analysis result of rapeseed active peptide is shown in Table 1, it can be seen that rapeseed active peptide prepared by the present invention
Total protein content is up to 98.2%, has very high product quality.
Table 1：The basic physical and chemical composition analysis of rapeseed active peptide
Rapeseed active peptide sample after sample introduction, is obtained into its gel chromatography figure, at liquid data in GEL-HPLC
The gel chromatography data of rapeseed active peptide are substituted into calibration curve equation and calculated by reason software, obtain the phase of the peptide of sample
To molecular mass and its distribution.The absorbing in human body of dipeptides, tripeptides in molecular weight 1000Da following components
Rate is high, has the higher nutritive value of specific ionization amino acid and physiological function.The present invention is calculated with areas of peak normalization method
The relative molecular mass distribution scope of rapeseed active peptide, as shown in table 2.The molecular weight it can be seen from molecular weight distribution result
Component less than 1000Da accounts for 91.45% altogether, if calculated by the mean molecule quantity 137Da of amino acid, molecular weight
Below 1000Da component is then the oligopeptide below octapeptide mostly, also including part free amino acid.Its middle-molecular-weihydroxyethyl exists
140Da-500Da's accounts for 60.23%, occupies below the 1000Da overwhelming majority, and this part is mainly dipeptides, tripeptides and four
Peptide.
Table 2：Rapeseed bioactive peptide molecule amount distribution results
3rd, Structural Identification quantifies with significant peptide fragment in rapeseed active peptide
Shown according to domestic and international newest nutrient research, after protein intake, be not fully hydrolyzed into amino acid, but big portion
Divide and digested and assimilated in the form of peptide by body.Peptide matters are in addition to trophic function that is easy to digest, easily absorbing, prior life
Thing meaning, which is mainly reflected in it, has the incomparable physiological active functionses of amino acid.Rapeseed active peptide mixes as a kind of
Property peptide, contain many peptide fragments with various physiological functions.Meanwhile component of its relative molecular mass less than 1000 accounts for 90%
More than, illustrate that this product is substantially made up of small peptide.Special protein raw materials source and unique production technology, make this product
Contain a certain amount of characteristic component peptide.
The peptide fragment in rapeseed active peptide is separated for we and Structural Identification.By using high performance liquid chromatography separation
With mass spectral analysis, the peptide fragment in this product is separated and Structural Identification, identify the primary structure of 42 peptide fragments altogether.
We have worked out tripeptides leucine-Tyr-Pro (Leu-Tyr- in quantitative determination rapeseed active peptide
Pro, LYP) high efficiency liquid phase chromatographic analysis method.By rapeseed active peptide sample after pre-treatment, using anti-phase C18 fillers as fixation
Phase, the difference according to sample component molecular polarity size are separated, detected under the conditions of UV absorption wavelength 220nm, are used
External standard method is quantified, and chromatogram and its data are handled, and LYP content is calculated.In view of the oil of different batches
Containing more than 2.5% LYP in vegetable seed active peptide sample, therefore using LYP as the characteristic chemical constituent in rapeseed active peptide.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (5)
1. a kind of preparation method of rapeseed active peptide, it is characterised in that comprise the following steps：
A, rapeseed is pre-processed, carries out ultramicro grinding, cross 200-600 mesh, with 100: 2~10 (L: kg) liquid ratio water and
Canola mixing is sized mixing, and is adjusted the micro- basifications of pH 8-10, is heated to 60~80 DEG C.30~120min of insulated and stirred；
B, the alkaline feed liquid in retort is pumped into centrifuge, is separated into clear liquid and slag charge, collect slag charge, slag charge plus water is dilute
Release, be heated to 60~80 DEG C, stir and separate, carried out 3 times with same processing mode；
C, the pH value of supernatant is adjusted to after 4-6, precipitation is collected in mechanical agitation 8-12h, centrifugation, precipitation as rapeseed protein,
It is repeated twice；
D, precipitation plus water are redissolved, is heated to 75-100 DEG C, time 15-45min so that protein denaturation, then enter horizontal high voltage
Homogeneous；
E, above-mentioned pH value of solution is adjusted to 5-10, adds special enzyme preparation：Trypsase: papain: neutral proteinase be 5~
7: 1~2: 3~5, hydrolysis temperature is 40-65 DEG C, time 1.5-8h；
F, after enzymolysis terminates, 90-125 DEG C of enzyme-removal temperature, the enzyme deactivation time is 1-3h, and thick enzymolysis liquid is obtained after terminating；
G：For the centrifugation of thick enzymolysis liquid, filtering, evaporation and concentration, addition activated carbon decolorizing, then spray drying are obtained into rapeseed work
Property Gly-His-Lys.
2. according to the method for claim 1, it is characterised in that trypsase in the step E: papain: neutral
The addition of protease is every gram of unit of protein 1000~2000.
3. according to the method for claim 1, it is characterised in that in the step G, using 0.05~0.1 μm of the film in aperture
Filter plant is filtered, and condition is 0.2~0.4MPa of pressure, 30~80 DEG C of temperature.
4. according to the method for claim 1, it is characterised in that in the step G, add concentrate total solids content
5% quality of activated carbon is decolourized.
5. a kind of preparation method of rapeseed active peptide, it is characterised in that described according to the preparation method described in claim 1
Rapeseed oligopeptide is characterized in that component of the molecular weight less than 1000Da accounts for more than 90%, and free aminoacid content is less than 5%,
And the tripeptides leucine at least containing 2.5% content-Tyr-Pro (Leu-Tyr-Pro, LYP) sequence peptide fragment.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101418329A (en) * | 2008-12-02 | 2009-04-29 | 江苏大学 | Preparation method of rapeseed proteolysis peptides based on pulse ultrasonic technology and use thereof |
| CN101701240A (en) * | 2009-11-12 | 2010-05-05 | 常州康和生物技术有限公司 | Method for preparing antihypertensive peptide by utilizing combined enzyme enzymolysis silkworm chrysalis protein |
| CN102174627A (en) * | 2011-01-12 | 2011-09-07 | 武汉百信食品有限公司 | Method for preparing rapeseed bioactive peptide |
| CN103173511A (en) * | 2011-12-23 | 2013-06-26 | 中国食品发酵工业研究院 | Method for industrially producing wheat glutamine peptide |
-
2017
- 2017-11-02 CN CN201711067183.9A patent/CN107873944A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101418329A (en) * | 2008-12-02 | 2009-04-29 | 江苏大学 | Preparation method of rapeseed proteolysis peptides based on pulse ultrasonic technology and use thereof |
| CN101701240A (en) * | 2009-11-12 | 2010-05-05 | 常州康和生物技术有限公司 | Method for preparing antihypertensive peptide by utilizing combined enzyme enzymolysis silkworm chrysalis protein |
| CN102174627A (en) * | 2011-01-12 | 2011-09-07 | 武汉百信食品有限公司 | Method for preparing rapeseed bioactive peptide |
| CN103173511A (en) * | 2011-12-23 | 2013-06-26 | 中国食品发酵工业研究院 | Method for industrially producing wheat glutamine peptide |
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