CN107860731A - A kind of specimen cup pivot analysis type spectrophotometer and analysis method - Google Patents
A kind of specimen cup pivot analysis type spectrophotometer and analysis method Download PDFInfo
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Abstract
The invention belongs to spectral information field of measuring technique, more particularly to a kind of specimen cup pivot analysis type spectrophotometer and analysis method.Its technical scheme is：Specimen cup is placed in sample cell；During detection, specimen cup is made to produce and rotate against with light beam producing system, detector, light beam caused by light beam producing system is circumferentially passed through the sample in it by setting interval angles along specimen cup, by each angle light energy of detector recording；The light energy for each angle that data handling system is recorded to detector is handled, and obtains the absorbance or transmitance value of sample.Instant invention overcomes light path of the cylindrical samples cup in all directions to have differences caused analytical error, ensure that the accuracy of sample analysis.
Description
Technical field
The invention belongs to spectral information field of measuring technique, more particularly to a kind of specimen cup pivot analysis type spectrophotometer
And analysis method.
Background technology
Spectrophotometer is also known as spectrometer, and it uses a light source that can produce multiple wavelength, is produced by monochromator
The light source of specific wavelength, sample to be analyzed are located in specimen cup, and after the light beam of specific wavelength passes through sample, some light is inhaled
Receive, by the degree of absorption of detector recording light beam, so as to draw the content of material in sample；In detection process, light beam passes through
The distance of sample is referred to as light path, and the length of light path is determined by the interior chamber size of specimen cup, and with the suction of sample to be analysed
Luminosity is directly proportional.
The type of specimen cup is broadly divided into the cuvette of cuboid or the colorimetric cylinder of cylinder；The cuvette of cuboid is by glass
Glass piece is assembled, and sheet glass can ensure the precision of light path by the attrition process of precision, and it is accurate to analyze sample using cuvette
Exactness is higher, but sample needs to be transferred in cuvette from test tube, and transfer process is complex.
The colorimetric cylinder of cylinder is often processed into using finished glass pipe, due to the limitation of processing technology, glass tube
It is difficult to ensure that consistent, light path of the colorimetric cylinder in all directions has differences the internal diameter of all directions；Analysis personnel are it is difficult to ensure that each
The secondary colorimetric cylinder that will fill sample, position when being put into the sample cell of spectrophotometer is definitely consistent, therefore analysis can be caused to tie
There is error, the impact analysis degree of accuracy in fruit.In order to overcome light path to have differences, operating personnel use and position mark are done on colorimetric cylinder
The method of note, this method need operating personnel's visual operation, and effect is also undesirable.
The content of the invention
It is an object of the invention to：Be directed to that light path of the colorimetric cylinder in all directions in the prior art have differences asks
Topic, there is provided a kind of specimen cup pivot analysis type spectrophotometer and analysis method.
The technical scheme is that：A kind of specimen cup pivot analysis type spectrophotometer, it includes：Light beam produces system
System, the specimen cup equipped with sample, detector and data handling system；
Specimen cup is placed in sample cell；During detection, specimen cup is produced and rotated against with light beam producing system, detector,
Light beam caused by light beam producing system is circumferentially passed through the sample in it by setting interval angles along specimen cup, by detector recording
Each angle light energy；The light energy for each angle that data handling system is recorded to detector is handled, and obtains the suction of sample
Luminosity or transmitance value.
In such scheme, specimen cup is produced and rotated against with two kinds of ways of realization with light beam producing system, detector；Its
Middle one kind is：Light beam producing system is fixedly installed with detector, and sample cell is connected with drive system, opens drive system, then sample
Product cup is rotated with sample cell by set angle.Another kind is：Sample cell is fixedly installed, and detector is pacified respectively with light beam producing system
Mounted in the both ends of support, support is connected with drive system, opens drive system, then detector and light beam producing system are around specimen cup
Rotated by set angle.
Further, position identification device is also provided with specimen cup, specimen cup is nearby provided with and position identification device
The position sensor to match；Interacted by position sensor and location indentifier, when recording the blank correction of specimen cup
Position；During detection, if specimen cup position is not inconsistent with position during blank correction, specimen cup is adjusted by drive system
The whole position to during blank correction.
The present invention another technical scheme be：A kind of specimen cup pivot analysis method, comprises the following steps：
A. two absorbances are chosen or transmitance is worth equal or similar specimen cup；
B. blank correction；
B1. it is put into after wherein adding blank solution in a specimen cup in sample cell, makes the specimen cup be produced with light beam
System, detector are produced and rotated against, and light beam caused by light beam producing system is by set angle along specimen cup circumferentially through sky
White solution, by detector by each anglec of rotation record light energy；The light for each angle that data handling system is recorded to detector
Energy is handled；
B2. according to result, blank correction is carried out to data handling system；
C. sample is detected；
It is put into after sample will be added in another specimen cup in sample cell, makes the specimen cup and light beam producing system, detection
Device is produced and rotated against, and light beam caused by light beam producing system circumferentially passes through sample by set angle along specimen cup, by detecting
Device records each anglec of rotation light energy；The light energy for each angle that data handling system is recorded to detector is handled, and is obtained
The absorbance of sample or transmitance value into the specimen cup.
The present invention the 3rd technical scheme be：A kind of specimen cup pivot analysis method, comprises the following steps：
A. a specimen cup is chosen；
B. blank correction；
B1. to being put into after adding blank solution in the specimen cup of selection in sample cell, light beam producing system is opened, by detecting
Device records current location light energy；Data handling system records light energy to detector and handled；
B2. according to result, blank correction is carried out to data handling system；
C. sample displacement；
Blank solution in specimen cup is poured out, is replaced by sample；
D. sample is detected；
Specimen cup equipped with sample is put into sample cell, makes the specimen cup produce phase with light beam producing system, detector
To rotation, light beam caused by light beam producing system circumferentially passes through sample by set angle along specimen cup, by detector by record
Each anglec of rotation light energy；The light energy that data handling system is recorded to detector is handled, and is obtained in the specimen cup
The absorbance or transmitance value of sample.
The present invention the 4th technical scheme be：A kind of specimen cup pivot analysis method, comprises the following steps：
A. a specimen cup is chosen；
B. position records；
The set location identifier on the specimen cup, specimen cup nearby set the position sensing to match with location indentifier
Device；Interacted by position sensor and location indentifier, record the position during blank correction of specimen cup；
C. blank correction；
C1. the specimen cup equipped with blank solution is put into sample cell, makes the specimen cup and light beam producing system, detector
Generation rotates against, and positions to the blank correction position of specimen cup, light beam producing system is opened, by detector recording current location
Light energy；Data handling system records light energy to detector and handled；
C2. according to result, blank correction is carried out to data handling system；
D. sample displacement；
Blank solution in the specimen cup is poured out, is replaced by sample；
E. sample is detected；
Specimen cup equipped with sample is put into sample cell, makes the specimen cup produce phase with light beam producing system, detector
To rotation, position to the position during blank correction of specimen cup；Light beam caused by light beam producing system is passed through in specimen cup
Sample, by detector recording specimen cup light energy；The light energy that data handling system is recorded to detector is handled, and is obtained
The absorbance of sample or transmitance value in the specimen cup.
Beneficial effect：The present invention is rotated against by making specimen cup be produced with light beam producing system, and record specimen cup is circumferential
The light energy of each position, and maximum value calculation therein is chosen by data handling system and obtains blank solution in the specimen cup
Absorbance or transmitance value, overcome light path of the cylindrical samples cup in all directions and have differences caused analytical error,
It ensure that the accuracy of sample analysis.
In another technical scheme of the present invention, by the interaction of location indentifier and position sensor, to sample
Cup is positioned.So that detecting each time, light beam can pass through specimen cup from same position, improve analysis repeatability, fully
The accuracy of analysis is ensured.
Brief description of the drawings
Fig. 1 is a concrete structure schematic diagram in embodiments of the invention 1；
Fig. 2 is another concrete structure schematic diagram in embodiments of the invention 1；
Fig. 3 is the concrete structure schematic diagram in embodiments of the invention 2.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
Embodiment 1
A kind of specimen cup pivot analysis type spectrophotometer, it includes：Light beam producing system 1, the specimen cup equipped with sample 3
2nd, detector 4 and data handling system 5；
Specimen cup 2 is placed in sample cell 6；During detection, specimen cup 2 produces relatively with light beam producing system 1, detector 4
Rotation, light beam caused by light beam producing system 1 is circumferentially passed through the sample 3 in it by setting interval angles along specimen cup 2, by examining
Survey device 4 and record each angle light energy；The light energy for each angle that data handling system 5 is recorded to detector 4 is handled, and is obtained
To the absorbance or transmitance value of sample 3.
The light energy for each angle that data handling system 5 is recorded to detector 4 is handled, and is obtained in the specimen cup 2
The absorbance of sample 3 or the specific method of transmitance value are：The light for each angle that data handling system 5 is recorded to detector 4
Energy is compared, and is chosen maximum value calculation therein and is obtained absorbance or transmitance value；Or：Data handling system 5 is to inspection
The light energy for surveying each angle that device 4 is recorded is averaged, and absorbance or transmitance value is calculated.
In such scheme, specimen cup is produced and rotated against with two kinds of ways of realization with light beam producing system, detector；
One of which is fixedly installed referring to accompanying drawing 1, light beam producing system 1 with detector 4, sample cell 6 and the phase of drive system 7
Even, drive system 7 is opened, then specimen cup 2 is rotated with sample cell 6 by set angle.
Another kind is fixedly installed referring to accompanying drawing 2, sample cell 6, and detector 4 is separately mounted to support 8 with light beam producing system 1
Both ends, support 8 is connected with drive system 7, opens drive system 7, then detector 4 is pressed with light beam producing system 1 around specimen cup 2
Set angle rotates.
Embodiment 2
Referring to accompanying drawing 3, on the basis of embodiment 1, further restriction is done to specimen cup 2：
Specimen cup 2 is provided with position identification device 10, and specimen cup 2 is nearby provided with the position to match with position identification device 10
Put sensor 9；Interacted by position sensor 9 and location indentifier 10, record the position during blank correction of specimen cup 2
Put；During detection, if the position of specimen cup 2 is not inconsistent with position during blank correction, specimen cup 2 is adjusted by drive system 7
The whole position to during blank correction.
Specific method during detection is：Specimen cup 2 equipped with sample 3 is put into sample cell 6, makes the specimen cup 2 and light
Beam generation system 1, detector 4 are produced and rotated against, and are positioned to the position during blank correction of specimen cup 2；Light beam producing system 1
Caused light beam records the light energy of specimen cup 2 through the sample 3 in specimen cup 2 by detector 4；Data handling system 5 is to inspection
Survey the light energy that device 4 is recorded to be handled, obtain the absorbance or transmitance value of sample 3 in the specimen cup 2.
Position identification device 10 and position sensor 9 in such scheme can be Photoinduction type, magnetic induction type, electromagnetic type or sound
Waves.
Embodiment 3
A kind of specimen cup pivot analysis method, the described method comprises the following steps：
A. two absorbances are chosen or transmitance is worth equal or similar specimen cup 2；
B. blank correction；
B1. it is put into after wherein adding blank solution in a specimen cup 2 in sample cell 6, makes the specimen cup 2 be produced with light beam
Raw system 1, detector 4 are produced and rotated against, and light beam caused by light beam producing system 1 is circumferential along specimen cup 2 by set angle
Through blank solution, by detector 4 by each anglec of rotation record light energy；Data handling system 5 is recorded each to detector 4
The light energy of angle is handled；
B2. according to result, blank correction is carried out to data handling system 5；
C. sample 3 is detected；
Be put into after sample 3 being added in another specimen cup 2 in sample cell 6, make the specimen cup 2 and light beam producing system 1,
Detector 4 is produced and rotated against, and light beam caused by light beam producing system 1 passes through sample by set angle along specimen cup 2 is circumferential
3, each anglec of rotation light energy is recorded by detector 4；The light energy for each angle that data handling system 5 is recorded to detector 4
Handled, obtain the absorbance or transmitance value of sample 3 in the specimen cup 2.
In above-mentioned steps B1 and C, at the light energy for each angle that data handling system 5 is recorded to detector 4
Reason, obtaining the absorbance of sample 3 or the specific method of transmitance value in the specimen cup 2 is：
The light energy for each angle that data handling system 5 is recorded to detector 4 is compared, and chooses maximum therein
Absorbance or transmitance value is calculated；
Or：
The light energy for each angle that data handling system 5 is recorded to detector 4 is averaged, be calculated absorbance or
Transmitance value.
Further：Step A concrete operation method is：
A1. multiple empty specimen cups 2 are prepared；Each specimen cup 2 is loaded into the check and correction sample with same absorbance, put by several times
Enter in sample cell 6, make specimen cup 2 produce and rotate against with light beam producing system 1, detector 4, produced by light beam producing system 1
Light beam by set angle along the circumferential extinction sample through in it of specimen cup 2, by detector 4 by recording each angle light energy；
The light energy of each angle recorded by data handling system 5 to detector 4 is handled, and obtains proofreading sample relative to air
Absorbance or transmitance value；
A2. two absorbances are chosen or transmitance is worth equal specimen cup 2, or the permissible value according to analytical error, are chosen
Two absorbances or transmitance are worth similar specimen cup 2.
Embodiment 4
A kind of specimen cup pivot analysis method, the described method comprises the following steps：
A. a specimen cup 2 is chosen；
B. blank correction；
B1. to being put into after adding blank solution in the specimen cup 2 of selection in sample cell 6, unlatching light beam producing system 1, by
Detector 4 records current location light energy；Data handling system 5 records light energy to detector 4 and handled；
B2. according to result, blank correction is carried out to data handling system 5；
C. sample displacement；
Blank solution in specimen cup 2 is poured out, is replaced by sample 3；
D. sample 3 is detected；
Specimen cup 2 equipped with sample 3 is put into sample cell 6, makes the specimen cup 2 and light beam producing system 1, detector 4
Generation rotates against, and light beam caused by light beam producing system 1 passes through sample 3 by set angle along specimen cup 2 is circumferential, by detecting
Device 4 is by recording each anglec of rotation light energy；The light energy that data handling system 5 is recorded to detector 4 is handled, and is obtained
The absorbance or transmitance value of sample 3 in the specimen cup 2.
In this example：The light energy for each angle that data handling system 5 is recorded to detector 4 is handled, and obtains the sample
The specific method of the absorbance of sample 3 or transmitance value is identical with method therefor in embodiment 3 in product cup 2.
Embodiment 5
A kind of specimen cup pivot analysis method, the described method comprises the following steps：
A. a specimen cup 2 is chosen；
B. position records；
The set location identifier 10 on the specimen cup 2, specimen cup 2 nearby set the position to match with location indentifier 10
Put sensor 9；Interacted by position sensor 9 and location indentifier 10, record the position during blank correction of specimen cup 2
Put；
C. blank correction；
C1. the specimen cup 2 equipped with blank solution is put into sample cell 6, makes the specimen cup 2 and light beam producing system 1, inspection
Survey the generation of device 4 to rotate against, position to the blank correction position of specimen cup 2, open light beam producing system 1, recorded by detector 4
Current location light energy；Data handling system 5 records light energy to detector 4 and handled；
C2. according to result, blank correction is carried out to data handling system 5；
D. sample displacement；
Blank solution in the specimen cup 2 is poured out, is replaced by sample 3；
E. sample 3 is detected；
Specimen cup 2 equipped with sample 3 is put into sample cell 6, makes the specimen cup 2 and light beam producing system 1, detector 4
Generation rotates against, and positions to the position during blank correction of specimen cup 2；Light beam caused by light beam producing system 1 passes through sample
Sample 3 in product cup 2, the light energy of specimen cup 2 is recorded by detector 4；The luminous energy that data handling system 5 is recorded to detector 4
Amount is handled, and obtains the absorbance or transmitance value of sample 3 in the specimen cup 2.
Although above with general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.
Claims (10)
1. a kind of specimen cup pivot analysis type spectrophotometer, the spectrophotometer include：Light beam producing system (1), it is equipped with
Specimen cup (2), detector (4) and the data handling system (5) of sample (3)；It is characterized in that：
The specimen cup (2) is placed in sample cell (6)；During detection, the specimen cup (2) and the light beam producing system (1),
The detector (4) produces and rotated against, and light beam caused by the light beam producing system (1) is as setting interval angles described in
Through the sample (3) in it, each angle light energy circumferentially is recorded by the detector (4) for specimen cup (2)；The data processing system
The light energy of each angle that system (5) is recorded to the detector (4) is handled, obtain the sample (3) absorbance or
Transmitance value.
A kind of 2. specimen cup pivot analysis type spectrophotometer as claimed in claim 1, it is characterised in that：The light beam produces
System (1) is fixedly installed with the detector (4), and the sample cell (6) is connected with the drive system (7), opens the drive
System (7) is moved, then the specimen cup (2) is rotated with the sample cell (6) by set angle.
A kind of 3. specimen cup pivot analysis type spectrophotometer as claimed in claim 1, it is characterised in that：The sample cell
(6) it is fixedly installed, the detector (4) is separately mounted to the both ends of support (8), the branch with the light beam producing system (1)
Frame (8) is connected with the drive system (7), opens the drive system (7), then the detector (4) produces with the light beam
System (1) is rotated around the specimen cup (2) by set angle.
A kind of 4. specimen cup pivot analysis type spectrophotometer as claimed in claim 2, it is characterised in that：The specimen cup
(2) position identification device (10) is provided with, the specimen cup (2) is nearby provided with what is matched with the position identification device (10)
Position sensor (9)；Interacted by the position sensor (9) and the location indentifier (10), record the sample
The position during blank correction of cup (2)；During detection, if the specimen cup (2) position is not inconsistent with position during blank correction,
Then the specimen cup (2) is adjusted to position during blank correction by the drive system (7).
A kind of 5. specimen cup pivot analysis type spectrophotometer as claimed in claim 4, it is characterised in that：The position identification
Device (10) and the position sensor (9) are Photoinduction type, magnetic induction type, electromagnetic type or sound wave type.
A kind of 6. specimen cup pivot analysis method, it is characterised in that：The analysis method comprises the following steps：
A. two absorbances are chosen or transmitance is worth equal or similar specimen cup (2)；
B. blank correction；
B1. it is put into after blank solution wherein being added in a specimen cup (2) in sample cell (6), makes the specimen cup (2) and light beam
Generation system (1), detector (4) are produced and rotated against, and light beam caused by light beam producing system (1) presses set angle along sample
Cup (2) circumferentially passes through blank solution, by detector (4) by each anglec of rotation record light energy；Data handling system (5) is to detection
The light energy for each angle that device (4) is recorded is handled；
B2. according to result, blank correction is carried out to data handling system (5)；
C. sample (3) is detected；
It is put into after sample (3) being added in another specimen cup (2) in sample cell (6), makes the specimen cup (2) produce system with light beam
System (1), detector (4) are produced and rotated against, and light beam caused by light beam producing system (1) presses set angle along specimen cup (2)
It is circumferential to pass through sample (3), each anglec of rotation light energy is recorded by detector (4)；Data handling system (5) is to detector (4) institute
The light energy of each angle of record is handled, and obtains the absorbance or transmitance value of sample (3) in the specimen cup (2).
A kind of 7. specimen cup pivot analysis method as claimed in claim 6, it is characterised in that：The concrete operations of the step A
Method is：
A1. multiple empty specimen cups (2) are prepared；Each specimen cup (2) is loaded into the check and correction sample with same absorbance, put by several times
Enter in sample cell (6), make specimen cup (2) produce and rotate against with light beam producing system (1), detector (4), light beam producing system
(1) light beam caused by is circumferentially passed through the extinction sample in it by set angle along specimen cup (2), by detector (4) by record
Each angle light energy；The light energy of each angle recorded by data handling system (5) to the detector (4) is handled,
Obtain proofreading absorbance or transmitance value of the sample relative to air；
A2. two absorbances are chosen or transmitance is worth equal specimen cup (2), or the permissible value according to analytical error, choose two
Absorbance or transmitance are worth similar specimen cup (2).
A kind of 8. specimen cup pivot analysis method as claimed in claims 6 or 7, it is characterised in that：The data handling system
(5) light energy of each angle recorded to detector (4) is handled, and obtains the absorbance of sample (3) in the specimen cup (2)
Or the specific method of transmitance value is：
The light energy for each angle that the data handling system (5) is recorded to the detector (4) is compared, and is chosen wherein
Maximum value calculation obtain absorbance or transmitance value；
Or：
The light energy for each angle that the data handling system (5) is recorded to the detector (4) is averaged, and is calculated
Absorbance or transmitance value.
A kind of 9. specimen cup pivot analysis method, it is characterised in that：The analysis method comprises the following steps：
A. a specimen cup (2) is chosen；
B. blank correction；
B1. to being put into after adding blank solution in the specimen cup (2) of selection in sample cell (6), unlatching light beam producing system (1),
Current location light energy is recorded by detector (4)；Data handling system (5) records light energy to detector (4) and handled；
B2. according to result, blank correction is carried out to data handling system (5)；
C. sample displacement；
Blank solution in specimen cup (2) is poured out, is replaced by sample (3)；
D. sample (3) is detected；
Specimen cup (2) equipped with sample (3) is put into sample cell (6), makes the specimen cup (2) and light beam producing system (1), inspection
Survey device (4) generation to rotate against, light beam caused by light beam producing system (1) is circumferentially passed through by set angle along specimen cup (2)
Sample (3), by detector (4) by recording each anglec of rotation light energy；Data handling system (5) is recorded to detector (4)
Light energy handled, obtain the absorbance or transmitance value of sample (3) in the specimen cup (2).
A kind of 10. specimen cup pivot analysis method, it is characterised in that：The analysis method comprises the following steps：
A. a specimen cup (2) is chosen；
B. position records；
The set location identifier (10) on the specimen cup (2), specimen cup (2) is nearby set to match with location indentifier (10)
Position sensor (9)；Interacted by position sensor (9) and location indentifier (10), the blank of record specimen cup (2)
The position of timing；
C. blank correction；
C1. the specimen cup (2) equipped with blank solution is put into sample cell (6), makes the specimen cup (2) and light beam producing system
(1), detector (4) is produced and rotated against, and is positioned to the blank correction position of specimen cup (2), is opened light beam producing system (1),
Current location light energy is recorded by detector (4)；Data handling system (5) records light energy to detector (4) and handled；
C2. according to result, blank correction is carried out to data handling system (5)；
D. sample displacement；
Blank solution in the specimen cup (2) is poured out, is replaced by sample (3)；
E. sample (3) is detected；
Specimen cup (2) equipped with sample (3) is put into sample cell (6), makes the specimen cup (2) and light beam producing system (1), inspection
Survey device (4) generation to rotate against, position to the position during blank correction of specimen cup (2)；Caused by light beam producing system (1)
Light beam records specimen cup (2) light energy through the sample (3) in specimen cup (2) by detector (4)；Data handling system (5) is right
The light energy that detector (4) is recorded is handled, and obtains the absorbance or transmitance value of sample (3) in the specimen cup (2).
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109060708A (en) * | 2018-10-19 | 2018-12-21 | 郑州轻工业学院 | A kind of novel hand held grain component fast analyser |
| CN110057788A (en) * | 2019-05-23 | 2019-07-26 | 上海景瑞阳实业有限公司 | Nano material absorbance test device, stationary phase test method, system and device |
| CN111929258A (en) * | 2020-09-01 | 2020-11-13 | 南京锦华机械制造有限公司 | Food chemical pollution detection device |
| CN114279990A (en) * | 2022-01-12 | 2022-04-05 | 北京华科仪科技股份有限公司 | Spectrophotometric measuring method |
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