CN104614335A - Method for rapidly identifying theabrownin - Google Patents
Method for rapidly identifying theabrownin Download PDFInfo
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- CN104614335A CN104614335A CN201510074729.8A CN201510074729A CN104614335A CN 104614335 A CN104614335 A CN 104614335A CN 201510074729 A CN201510074729 A CN 201510074729A CN 104614335 A CN104614335 A CN 104614335A
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Abstract
The invention discloses a method for rapidly identifying theabrownin. The method comprises the following steps: (1) qualitative detection, namely, after preparing a theabrownin solution, detecting an absorbance of the theabrownin solution by using an ultramicro ultraviolet spectrophotometer to obtain a characteristic peak pattern of the theabrownin; and (2) quantitative detection, namely, after preparing the theabrownin solution, detecting an absorption peak of the theabrownin solution under a specific wavelength of 273nm, and calculating the content of the theabrownin by virtue of the peak area or peak height of the absorption peak. The method for rapidly identifying the theabrownin, disclosed by the invention, has the beneficial effects of accurate detection result, short detection time, little influence of artificial factors, small difference between determined batches, small detection workload, and standardized detecting steps.
Description
Technical field
The present invention relates to chemical identification field, particularly a kind of method of Rapid identification theabrownin.
Background technology
Existing theabrownin (Tea Pigment) authentication method is divided into quantitative and qualitative analysis two kinds, traditional uv absorption quilitative method adopts common spectrophotometer, theabrownin absorption peak is 273nm ± 20nm, traditional colorimetric determination based on typical curve needs standard items, in view of theabrownin (Tea Pigment) is a mixing polymer, so there is no the standard items of real meaning, and the running time long (more than 1 hour), each typical curve all needs again to do, the value application condition measured by the method is large, even there will be the situation of content more than 100%.
Newly there is a kind of detection method adopting the method for high performance liquid chromatography or mass spectrum or liquid-mass chromatography to carry out theabrownin now, the data obtained is accurate, but also there are some defects, as machine is expensive, speed is slow, high to operating personnel's skill set requirements, high performance liquid chromatography is too accurate, and measured result is widely different.
Summary of the invention
In order to solve as above technical matters, the invention provides a kind of method of Rapid identification theabrownin, the method has detection quick and precisely, is affected by human factors little, the feature that workload is little.
The method of a kind of Rapid identification theabrownin of the present invention, comprises the steps:
1) qualitative detection: after preparing dark brown cellulose solution, uses the absorbance of ultramicron UV spectrophotometer measuring theabrownin solution, obtains characteristic peak and the characteristic peak type of theabrownin;
2) quantitatively detect: preparation concentration known theabrownin solution, uses capillary electrophoresis apparatus to detect the absorption peak of theabrownin solution, and by absorption peak peak area or peak height drawing standard curve and formula; Then detect theabrownin solution to be measured, reference standard curve calculates theabrownin content to be measured.
Further, described step 1) to be specially preparation theabrownin concentration be after the theabrownin solution of 0.5-8g/L, get the absorbance that 2-5 μ L theabrownin solution detects theabrownin under the 200-850nm wavelength of ultramicron ultraviolet spectrophotometer, the peak type that theabrownin occurs characteristic absorption peak and determines in this wavelength coverage.
Further, described step 2) be specially preparation 5-20 variable concentrations and the content that concentration is 0.5-8g/L is after the theabrownin solution of 90%, the absorption peak that 2-10ml theabrownin solution detects theabrownin in capillary electrophoresis apparatus is got in the solution of each concentration, by peak area or the peak height of absorption peak, calculate theabrownin content, and matching concentration-peak area typical curve:
y＝0.000003679～0.000011037x±0.093063315；
Or concentration-peak height typical curve:
y＝0.000126115～0.000378345x±0.039633517；
Wherein the testing conditions of capillary electrophoresis apparatus is: buffer solution is borax, and capillary pipe length is 30-60cm, and electrophoresis time is 700-1200s, and sample introduction pressure is 0.5-2kpa, and flushing pressure is 20-50kpa, UV-detector determined wavelength 273nm;
The theabrownin sample to be measured being 0.5-8g/L by concentration with same deposition condition carries out Capillary Electrophoresis and obtains peak area or peak height, calculates relative concentration, finally obtain the content of theabrownin sample to be measured according to concentration-peak area or concentration-peak height typical curve.
Whether the method for above-mentioned Rapid identification theabrownin is first detected containing theabrownin in solution by qualitative detection, more quantitatively detects the solution containing theabrownin, by calculating the relative concentration of theabrownin, and then obtains the content of this theabrownin sample; This qualitative detection and quantitatively detecting combines and in 30min, accurately can measure theabrownin content to the method that theabrownin solution detects, and error is only 5%-10%, is significantly less than in the past to the error rate of the detection method of theabrownin.
Further, a kind of method of Rapid identification theabrownin, the method is: after preparing dark brown cellulose solution, uses the absorbance of ultramicron UV spectrophotometer measuring theabrownin solution, obtains characteristic peak and the characteristic peak type of theabrownin.
Further, it is after the theabrownin solution of 0.5-8g/L that described method is specially preparation theabrownin concentration, get the absorbance that 2-5 μ L theabrownin solution detects theabrownin under the 200-850nm wavelength of ultramicron ultraviolet spectrophotometer, the peak type that theabrownin occurs characteristic absorption peak and determines in this wavelength coverage.
When only needing the qualitative detection of solution being carried out to theabrownin, when not needing quantitatively to detect, said method of the present invention can be adopted, the method is qualitative detection, this qualitative detection is detected by ultramicron ultraviolet spectrophotometer, and the theabrownin characteristic peak recorded is at 273nm ± 2nm place and all-wave long peak type is unique.By the method, the uncertain solution whether containing theabrownin is detected, if there is characteristic peak at 273nm ± 2nm place, then illustrate containing theabrownin in this solution, if without this characteristic peak, then illustrate in this solution not containing theabrownin.If peak type diagram shape is inconsistent, in interpret sample, other materials may be contained.
The method of above-mentioned Rapid identification theabrownin is only qualitative detection, can judge that detection time is short, and testing result is accurate whether containing theabrownin in solution in 5min.
To sum up, the beneficial effect that the method for a kind of Rapid identification theabrownin of the present invention has has: testing result is accurate, and detection time is short; Be affected by human factors little, the difference between batch of mensuration is little; Testing amount is little; Detecting step standardization.
Accompanying drawing explanation
Fig. 1 is ultramicron ultraviolet spectrophotometer absorbance peak type figure in embodiment 1;
Fig. 2 is the electrophoretogram that in embodiment 1, capillary electrophoresis apparatus detects;
Fig. 3 is ultramicron ultraviolet spectrophotometer absorbance peak type figure in embodiment 2;
Fig. 4 is ultramicron ultraviolet spectrophotometer absorbance peak type figure in embodiment 3;
Fig. 5 is the electrophoretogram that in embodiment 3, capillary electrophoresis apparatus detects;
Embodiment
The technological means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with concrete diagram, setting forth the present invention further.
Embodiment 1
The method of a kind of Rapid identification theabrownin of the present invention, comprises the steps:
1) qualitative detection: preparation theabrownin concentration is after the theabrownin solution of 1g/L, get the absorbance that 3 μ L theabrownin solution detect theabrownin under the 200-850nm wavelength of k5600 type ultramicron ultraviolet spectrophotometer, there is characteristic absorption peak at 273nm ± 2nm place in theabrownin, Fig. 1 is shown in by UV-VIS full wavelength scanner peak type;
2) quantitatively detect: compound concentration is after the theabrownin solution to be measured of 4g/L, getting the absorption peak that 2ml theabrownin solution detects theabrownin in capillary electrophoresis apparatus is 496062uV.S, wherein deposition condition is: buffer solution is borax, capillary pipe length 50cm, electrophoresis time 900s, sample introduction pressure 1kpa, flushing pressure 40kpa, UV-detector determined wavelength is 273nm, and electrophoretogram is shown in Fig. 2.
The relative concentration being calculated theabrownin to be measured by concentration-peak area typical curve formula y=0.000007358*x+0.06204221 is 3.712g/L, and finally obtaining theabrownin content to be measured is 90%* (3.712/4)=83.5%;
The beneficial effect that the method for a kind of Rapid identification theabrownin of the present embodiment has has: testing result is accurate, and detection time is short; Be affected by human factors little, the difference between batch of mensuration is little; Testing amount is little; Detecting step standardization.
Embodiment 2
The method of a kind of Rapid identification theabrownin of the present invention, the method is:
Preparation theabrownin concentration is after the theabrownin solution of 2g/L, get the absorbance that 4 μ L theabrownin solution detect theabrownin under the 200-850nm wavelength of k5600 type ultramicron ultraviolet spectrophotometer, there is characteristic absorption peak at 273nm ± 2nm place in theabrownin, Fig. 3 is shown in by UV-VIS full wavelength scanner peak type;
The beneficial effect that the method for a kind of Rapid identification theabrownin of the present embodiment has has: testing result is accurate, and detection time is short; Be affected by human factors little, the difference between batch of mensuration is little; Testing amount is little; Detecting step standardization.
Embodiment 3
The method of a kind of Rapid identification theabrownin of the present invention, comprises the steps:
1) qualitative detection: preparation theabrownin concentration is after the theabrownin solution of 2g/L, get the absorbance that 2 μ L theabrownin solution detect theabrownin under the 200-850nm wavelength of k5600 type ultramicron ultraviolet spectrophotometer, there is characteristic absorption peak at 273nm ± 2nm place in theabrownin, Fig. 4 is shown in by UV-VIS full wavelength scanner peak type;
2) quantitatively detect: compound concentration is after the theabrownin solution to be measured of 6g/L, getting the peak height that 3ml theabrownin solution detects theabrownin in capillary electrophoresis apparatus is 20679mV, wherein deposition condition is: buffer solution is borax, capillary pipe length 50cm, electrophoresis time 900s, sample introduction pressure 1kp, flushing pressure 40kpa, UV-detector determined wavelength is 273nm, and electrophoretogram is shown in Fig. 5.
The relative concentration being calculated theabrownin to be measured by concentration-peak height typical curve formula y=0.00025223*x+0.026422345 is 5.242g/L, and finally obtaining theabrownin content to be measured is 90%* (3.712/4)=78.6%;
The beneficial effect that the method for a kind of Rapid identification theabrownin of the present embodiment has has: testing result is accurate, and detection time is short; Be affected by human factors little, the difference between batch of mensuration is little; Testing amount is little; Detecting step standardization.
Claims (5)
1. a method for Rapid identification theabrownin, is characterized in that, comprises the steps:
1) qualitative detection: after preparing dark brown cellulose solution, uses the absorbance of ultramicron UV spectrophotometer measuring theabrownin solution, obtains the characteristic peak type of theabrownin;
2) quantitatively detect: preparation concentration known theabrownin solution, uses capillary electrophoresis apparatus to detect the absorption peak of theabrownin solution under specific wavelength 273nm, and by absorption peak peak area or peak height drawing standard curve and formula; Then detect theabrownin solution to be measured, reference standard curve calculates theabrownin content to be measured.
2. the method for a kind of Rapid identification theabrownin according to claim 1, it is characterized in that: described step 1) to be specially preparation theabrownin concentration be after the theabrownin solution of 0.5-8g/L, get the absorbance that 2-5 μ L theabrownin solution detects theabrownin under the 200-850nm wavelength of ultramicron ultraviolet spectrophotometer, the peak pattern that theabrownin occurs characteristic absorption peak and determines in this wavelength coverage.
3. the method for a kind of Rapid identification theabrownin according to claim 1, it is characterized in that: described step 2) be specially preparation 5-20 variable concentrations and the content that concentration is 0.5-8g/L is after the theabrownin solution of 90%, get 2-10ml theabrownin solution in the solution of each concentration in capillary electrophoresis apparatus, detect the absorption peak of theabrownin at 273nm place, by peak area or the peak height of absorption peak, calculate theabrownin content; Wherein the testing conditions of capillary electrophoresis apparatus is: buffer solution is borax, and capillary pipe length is 30-60cm, and electrophoresis time is 700-1200s, and sample introduction pressure is 0.5-2kpa, and flushing pressure is 20-50kp;
Adopt the theabrownin sample to be measured being 0.5-8g/L by concentration with above-mentioned deposition condition to carry out Capillary Electrophoresis and obtain peak area or peak height, calculate relative concentration according to concentration-peak area or concentration-peak height typical curve, finally obtain the content of theabrownin sample to be measured.
4. a method for Rapid identification theabrownin according to claim 1, is characterized in that, the method is: after preparing dark brown cellulose solution, uses the absorbance of ultramicron UV spectrophotometer measuring theabrownin solution, obtains characteristic peak and the peak figure of theabrownin.
5. the method for a kind of Rapid identification theabrownin according to claim 4, it is characterized in that: it is after the theabrownin solution of 0.5-8g/L that described method is specially preparation theabrownin concentration, get the absorbance that 2-5 μ L theabrownin solution detects theabrownin under the 200-850nm wavelength of ultramicron ultraviolet spectrophotometer, the peak type that theabrownin occurs characteristic absorption peak and determines in this wavelength coverage.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106344715A (en) * | 2016-08-26 | 2017-01-25 | 杭州茗褐生物科技有限公司 | Application of theabrownin to senescence resistance |
| CN110887713A (en) * | 2019-12-04 | 2020-03-17 | 湖南农业大学 | Dark tea brown reference substance and application thereof |
| CN115950845A (en) * | 2023-03-09 | 2023-04-11 | 国能龙源环保有限公司 | Method for determining tetravalent vanadium content in pickle liquor of waste denitration catalyst |
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| CN103704422A (en) * | 2013-12-23 | 2014-04-09 | 张松波 | Method for preparing and extracting theabrownin by using tea leaves as raw material |
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2015
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| US20070243622A1 (en) * | 2006-03-28 | 2007-10-18 | Georgia State University Research Foundation, Inc. | Polymeric sulfated surfactants for capillary electrophoresis (ce) and ce-mass spectrometry (ce-ms) |
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Cited By (3)
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| CN106344715A (en) * | 2016-08-26 | 2017-01-25 | 杭州茗褐生物科技有限公司 | Application of theabrownin to senescence resistance |
| CN110887713A (en) * | 2019-12-04 | 2020-03-17 | 湖南农业大学 | Dark tea brown reference substance and application thereof |
| CN115950845A (en) * | 2023-03-09 | 2023-04-11 | 国能龙源环保有限公司 | Method for determining tetravalent vanadium content in pickle liquor of waste denitration catalyst |
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Effective date of registration: 20170105 Address after: 311100, No. 18 Haishu Road, Hangzhou street, Yuhang District, Zhejiang, China A212 Applicant after: Hangzhou Ming Brown Biological Technology Co. Ltd. Address before: Hangzhou City, Zhejiang province 310000 Wensan Road No. 499 Xixi front is 3 building 103 Applicant before: Zhang Jin |
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