CN107858288A - A kind of sterile type stem cell culturing apparatus - Google Patents
A kind of sterile type stem cell culturing apparatus Download PDFInfo
- Publication number
- CN107858288A CN107858288A CN201711411130.4A CN201711411130A CN107858288A CN 107858288 A CN107858288 A CN 107858288A CN 201711411130 A CN201711411130 A CN 201711411130A CN 107858288 A CN107858288 A CN 107858288A
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- China
- Prior art keywords
- stem cell
- culture dish
- housing
- rubber blanket
- cover plate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of sterile type stem cell culturing apparatus, including housing, cover plate is provided with above the housing, the enclosure interior is provided with culture dish, the culture dish is internally connected with dividing plate, ceramic wafer is provided with below the culture dish, support base is connected with below the ceramic wafer, enclosure interior bottom is fixedly connected on below the support base, electrically-heated coil is provided with inside the support base, the cover plate is provided with opening groove, and end cap is connected with above the opening groove, and the opening groove is internally connected with the first limit card block.By opening end cap, the first rubber blanket and the second rubber blanket are pierced through to culture dish internal injection stem cell using syringe, first rubber blanket and the second rubber blanket can completely cut off the air of outside, it is also the bacterium for having completely cut off the external world simultaneously, so that stem cell is to be completed completely among sterile environment when injection, avoid bacterium to enter among culture dish so that experiment is disturbed to lead to the failure.
Description
Technical field
The present invention relates to cell cultivation equipment technical field, specially a kind of sterile type stem cell culturing apparatus.
Background technology
Culture dish is a kind of labware for being used for microorganism or cell culture, by the bottom and one of a flat circle plate-like
Individual lid composition, is typically made of glass or plastics.Culture dish material is essentially divided into two classes, predominantly plastic and glass, glass
The adhere-wall culture that can be used for vegetable material, microculture and zooblast of glass may also be used.Plastics are probably poly-
Vinyl material, once property and it is nonexpondable, be adapted to laboratory inoculation, line, the operation of separation of bacterial, can be used for
The culture of vegetable material.
Culture dish can inject stem cell when stem cell culture is done on the culture medium for supporting ware and be cultivated, still
Stem cell may carry during injecting because the bacterium in air, which is fallen into culture dish, causes stem cell culture failure
Extremely it is necessary to solve above-mentioned produced problem for a kind of sterile type stem cell culturing apparatus.
The content of the invention
It is an object of the invention to provide a kind of sterile type stem cell culturing apparatus, to solve to propose in above-mentioned background technology
The problem of.
To achieve the above object, the present invention provides following technical scheme：A kind of sterile type stem cell culturing apparatus, including shell
Body, the housing top are provided with cover plate, and the enclosure interior is provided with culture dish, and the culture dish is internally connected with dividing plate, described
Ceramic wafer is provided with below culture dish, support base is connected with below the ceramic wafer, housing is fixedly connected on below the support base
Inner bottom, the support base inside are provided with electrically-heated coil, and the cover plate is provided with opening groove, connected above the opening groove
End cap is connected to, the opening groove is internally connected with the first limit card block, the first rubber is connected with above first limit card block
Rubber cushion, the first rubber blanket top are provided with the second limit card block, the second rubber blanket are connected with above second limit card block.
Preferably, the cover plate bottom is provided with the first groove, and the second groove is provided among the cover plate.
Preferably, the housing side is provided with observation window, and the housing opposite side is provided with temperature detect switch (TDS).
Preferably, the cover plate bottom, culture dish upper end and dividing plate upper end are respectively connected with sealing gasket.
Preferably, the enclosure interior both sides are connected with LED.
Preferably, the end cap bottom is connected with rubber stopper.
Compared with prior art, the beneficial effects of the invention are as follows：The present invention pierces through the by opening end cap, using syringe
One rubber blanket and the second rubber blanket can completely cut off outside to culture dish internal injection stem cell, the first rubber blanket and the second rubber blanket
Air, while be also completely cut off the external world bacterium so that stem cell inject when be completely complete among sterile environment
Into avoiding bacterium and enter among culture dish so that experiment is disturbed to be led to the failure, and culture dish is heated by electrically-heated coil,
Temperature detect switch (TDS) controls the cultivation temperature of enclosure interior, and ceramic wafer has the effect of preferably conduction heat so that culture dish is heated fast
Fast and uniform, LED is then that illumination is provided when being observed by observation window, and the effect of dividing plate is that culture dish is divided into two
Part provides control group.Structure of the present invention is novel, effectively can completely cut off in the experimentation of stem cell injection in outside air
Bacterium so that experiment will not be destroyed by bacterium, and experimentation can provide the cultivation temperature of needs, be improved real
The probability of success tested.
Brief description of the drawings
Fig. 1 is overlooking the structure diagram of the present invention；
Fig. 2 is A-A cross-sectional views of the present invention；
Fig. 3 is part B partial enlargement structural representation of the present invention；
Fig. 4 is C portion partial enlargement structural representation of the present invention.
In figure：1 housing, 2 cover plates, 3 culture dishes, 4 dividing plates, 5 support bases, 6 electrically-heated coils, 7 ceramic wafers, 8 opening grooves, 9
End cap, 10 first limited location snap rings, 11 first rubber blankets, 12 second limited location snap rings, 13 second rubber blankets, 14 rubber stoppers, 15 first
Groove, 16 second grooves, 17 sealing gaskets, 18 LEDs, 19 temperature detect switch (TDS)s, 20 observation windows.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
Fig. 1-4 are referred to, the present invention provides a kind of technical scheme：A kind of sterile type stem cell culturing apparatus, including housing
1, the top of housing 1 is provided with cover plate 2, and the inside of housing 1 is provided with culture dish 3, and the culture dish 3 is internally connected with dividing plate 4,
The lower section of culture dish 3 is provided with ceramic wafer 7, and the lower section of ceramic wafer 7 is connected with support base 5, and the lower section of support base 5 is fixed to be connected
The inner bottom of housing 1 is connected to, the inside of support base 5 is provided with electrically-heated coil 6, and the cover plate 2 is provided with opening groove 8, described
The top of opening groove 8 is connected with end cap 9, and the opening groove 8 is internally connected with the first limit card block 10, first limiting card
The top of block 10 is connected with the first rubber blanket 11, and the top of the first rubber blanket 11 is provided with the second limit card block 12, second limit
The top of position fixture block 12 is connected with the second rubber blanket 13.
The bottom of cover plate 2 is provided with the first groove 15, and the second groove 16 is provided among the cover plate 2.First groove 15 with
The setting of second groove 16 is so that cover plate 2 can be stuck with culture dish 3 and dividing plate 4, forms sealing space.The housing 1 one
Side is provided with observation window 20, and the opposite side of housing 1 is provided with temperature detect switch (TDS) 19.The bottom of cover plate 2, the upper end of culture dish 3 and the dividing plate 4
Upper end is respectively connected with sealing gasket 17.The inside both sides of housing 1 are connected with LED 18.The setting of LED 18 is so that observation training
Can provide illumination observation when supporting 3 internal state of ware must become apparent from.The bottom of end cap 9 is connected with rubber stopper 14.
Operation principle：By opening end cap 9, the first rubber blanket 11 and the second rubber blanket 13 are pierced through to culture using syringe
The internal injection stem cell of ware 3, the first rubber blanket 11 and the second rubber blanket 13 can completely cut off the air of outside, while be also isolation
Extraneous bacterium so that stem cell is to be completed completely among sterile environment when injection, avoids bacterium and enters training
Among foster ware 3 so that experiment is disturbed to lead to the failure, and passes through electrically-heated coil 6 and heats culture dish 3, temperature detect switch (TDS) 19 controls housing 1
Internal cultivation temperature, ceramic wafer 7 have the effect of more preferable conduction heat so that culture dish 3 is heated quick and uniform, LED
Lamp 18 is then that illumination is provided when being observed by observation window 20, and the effect of dividing plate 4 is that culture dish 3 is divided to provide for two parts
Control group.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of sterile type stem cell culturing apparatus, including housing (1), it is characterised in that：Cover plate is provided with above the housing (1)
(2) culture dish (3), is provided with inside the housing (1), the culture dish (3) is internally connected with dividing plate (4), the culture dish (3)
Lower section is provided with ceramic wafer (7), and support base (5) is connected with below the ceramic wafer (7), is fixedly connected below the support base (5)
Electrically-heated coil (6) is provided with inside housing (1) inner bottom, the support base (5), the cover plate (2) is provided with opening groove
(8) end cap (9), is connected with above the opening groove (8), the opening groove (8) is internally connected with the first limit card block
(10) the first rubber blanket (11), is connected with above first limit card block (10), is provided with above first rubber blanket (11)
Second limit card block (12), the second limit card block (12) top are connected with the second rubber blanket (13).
A kind of 2. sterile type stem cell culturing apparatus according to claim 1, it is characterised in that：Cover plate (2) bottom
Provided with the first groove (15), the second groove (16) is provided among the cover plate (2).
A kind of 3. sterile type stem cell culturing apparatus according to claim 1, it is characterised in that：Housing (1) side
Provided with observation window (20), housing (1) opposite side is provided with temperature detect switch (TDS) (19).
A kind of 4. sterile type stem cell culturing apparatus according to claim 1, it is characterised in that：Cover plate (2) bottom,
Culture dish (3) upper end and dividing plate (4) upper end are respectively connected with sealing gasket (17).
A kind of 5. sterile type stem cell culturing apparatus according to claim 1, it is characterised in that：The housing (1) is internal
Both sides are connected with LED (18).
A kind of 6. sterile type stem cell culturing apparatus according to claim 1, it is characterised in that：End cap (9) bottom
It is connected with rubber stopper (14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711411130.4A CN107858288A (en) | 2017-12-23 | 2017-12-23 | A kind of sterile type stem cell culturing apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711411130.4A CN107858288A (en) | 2017-12-23 | 2017-12-23 | A kind of sterile type stem cell culturing apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107858288A true CN107858288A (en) | 2018-03-30 |
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ID=61707225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711411130.4A Pending CN107858288A (en) | 2017-12-23 | 2017-12-23 | A kind of sterile type stem cell culturing apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107858288A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205295354U (en) * | 2016-01-12 | 2016-06-08 | 段爱云 | Immunity bacteria cultivation of clinical laboratory device |
| CN205839026U (en) * | 2016-05-04 | 2016-12-28 | 威海地宝生物科技有限公司 | A kind of microorganism is cultivated with the visual thermostat with breeding function |
| CN207581823U (en) * | 2017-12-06 | 2018-07-06 | 李鑫 | A kind of fungal detection kit |
-
2017
- 2017-12-23 CN CN201711411130.4A patent/CN107858288A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205295354U (en) * | 2016-01-12 | 2016-06-08 | 段爱云 | Immunity bacteria cultivation of clinical laboratory device |
| CN205839026U (en) * | 2016-05-04 | 2016-12-28 | 威海地宝生物科技有限公司 | A kind of microorganism is cultivated with the visual thermostat with breeding function |
| CN207581823U (en) * | 2017-12-06 | 2018-07-06 | 李鑫 | A kind of fungal detection kit |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180330 |