CN107857770A - A kind of method of enzymolysis and extraction qinghaosu - Google Patents
A kind of method of enzymolysis and extraction qinghaosu Download PDFInfo
- Publication number
- CN107857770A CN107857770A CN201711415169.3A CN201711415169A CN107857770A CN 107857770 A CN107857770 A CN 107857770A CN 201711415169 A CN201711415169 A CN 201711415169A CN 107857770 A CN107857770 A CN 107857770A
- Authority
- CN
- China
- Prior art keywords
- qinghaosu
- filter cake
- organic solvent
- filtrate
- washed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
- C07D493/12—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains three hetero rings
- C07D493/20—Spiro-condensed systems
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of method of enzymolysis and extraction qinghaosu, comprise the following steps：1）Stock, after artemisia leaf is crushed, dilution heat of sulfuric acid or dilute hydrochloric acid solution that 21 times of weight pH value are 1, the aqueous solution and a certain amount of enzyme preparation are added, heated at constant temperature 25min, is cooled down, filtering, filtrate is extracted repeatedly with a kind of organic solvent in ether or petroleum ether；2）Heating, the organic solvent of 110 times of weight is added toward above-mentioned filter cake, it is 61 DEG C, heated at constant temperature 25min to control temperature, is cooled down, and filtering, filter cake is washed repeatedly with organic solvent, and filter cake discards, and filtrate and cleaning solution merge；3）Merge, combining step 1）With step 2）Gained extract, cleaning solution and filtrate, are washed with 2% sodium hydroxide solution, and neutrality is washed to distillation, are concentrated under reduced pressure in 50 DEG C, obtain qinghaosu crude product.Crude product passes through ethyl alcohol recrystallization, obtains qinghaosu highly finished product, and the present invention has the advantages of preparation technology is simple, purification efficiency is higher, preparation cost is relatively low in a word.
Description
Technical field
The invention belongs to qinghaosu preparation technology field, and in particular to a kind of method of enzymolysis and extraction qinghaosu.
Background technology
Qinghaosu is a kind of colourless acicular crystal for extracting to obtain from compound inflorescence plant Artemisia annua i.e. Chinese medicine artemisia leaf,
Drug containing sweet wormwood, qinghaosu are not the maximally effective antimalarial specific drugs after pyrimethamine, chloroquine, primary quinoline in its stem, especially
For encephalic malaria and anti-chlorine quinoline malaria, there is the characteristics of quick-acting and less toxic, be once referred to as " in the world only by the World Health Organization
One effective malaria treatment medicine "；The anti-malarial mechanism of action essentially consists in passes through qinghaosu activation production in the process for treating malaria
Raw free radical, free radical and malaria original protein binding, act on the film structure of plasmodium, make its vacuolar membrane, nuclear membrane and plasma membrane equal
Destroyed, mitochondrial swelling, interior outer membrane comes off, so as to be damaged to the eucaryotic cell structure and its function of plasmodium；In addition it is blue or green
Punt-pole element also shows that tempting prospect in the treatment of other diseases；As anti-schistosome, regulation or suppress body fluid immunologic function,
The conversion ratio of lymphocyte is improved, cholagogic, eliminating the phlegm, antibechic, is relievingd asthma, therefore qinghaosu has great medical market prospect；
Traditional extraction method is extracted repeatedly with organic solvent, and waste of solvent is serious, and recovery rate and raw material availability are relatively low；And shooting flow
Though body method recovery rate is of a relatively high, also because equipment is expensive, popularization is extremely restricted, it is therefore necessary to the extraction to qinghaosu
Method is improved.
The content of the invention
The invention aims to overcome the deficiencies in the prior art, and provide a kind of method of enzymolysis and extraction qinghaosu.
The object of the present invention is achieved like this：A kind of method of enzymolysis and extraction qinghaosu, comprises the following steps：
1）Stock, after artemisia leaf is crushed, add dilution heat of sulfuric acid or dilute hydrochloric acid solution that 21 times of weight pH value are 1, the aqueous solution
With a certain amount of enzyme preparation, it is 61 DEG C, heated at constant temperature 25min to control temperature, is cooled down, filtering, in filtrate ether or petroleum ether
A kind of organic solvent extract repeatedly, it is standby after filter cake low temperature drying；
2）Heating, the organic solvent of 110 times of weight is added toward above-mentioned filter cake, control temperature as 61 DEG C, heated at constant temperature 25min is cold
But, filter, filter cake is washed repeatedly with organic solvent, and filter cake discards, and filtrate and cleaning solution merge；
3）Merge, combining step 1）With step 2）Gained extract, cleaning solution and filtrate, carried out with 2% sodium hydroxide solution
Washing, is washed to neutrality with distillation, is concentrated under reduced pressure in 50 DEG C, obtain qinghaosu crude product.Crude product passes through ethyl alcohol recrystallization, obtains qinghaosu
Highly finished product.
Beneficial effects of the present invention：The inventive method has time-consuming short, recovery rate height, purity height and the low advantage of power consumption,
It is the extracting method of a kind of green and efficient qinghaosu, the present invention is with preparation technology is simple, purification efficiency is higher, system in a word
Standby lower-cost advantage.
Embodiment
Embodiment
A kind of method of enzymolysis and extraction qinghaosu, comprises the following steps：
1）Stock, after artemisia leaf is crushed, add dilution heat of sulfuric acid or dilute hydrochloric acid solution that 21 times of weight pH value are 1, the aqueous solution
With a certain amount of enzyme preparation, it is 61 DEG C, heated at constant temperature 25min to control temperature, is cooled down, filtering, in filtrate ether or petroleum ether
A kind of organic solvent extract repeatedly, it is standby after filter cake low temperature drying；
2）Heating, the organic solvent of 110 times of weight is added toward above-mentioned filter cake, control temperature as 61 DEG C, heated at constant temperature 25min is cold
But, filter, filter cake is washed repeatedly with organic solvent, and filter cake discards, and filtrate and cleaning solution merge；
3）Merge, combining step 1）With step 2）Gained extract, cleaning solution and filtrate, carried out with 2% sodium hydroxide solution
Washing, is washed to neutrality with distillation, is concentrated under reduced pressure in 50 DEG C, obtain qinghaosu crude product.Crude product passes through ethyl alcohol recrystallization, obtains qinghaosu
Highly finished product.
The present invention is when implementing, and in the extraction process of active skull cap components, solvent spreads to the surface of solids first, infiltration
To the surface of solids, then enter dissolving active skull cap components in people's solid interior and its micropore, finally by solid microporous gap to
The surface of solids spreads, and between the surface of solids and solvent main body under concentration difference active force, active skull cap components expand to solvent main body
Dissipate, complete extraction mass transport process.By qinghaosu almost by cellulose form cell membrane surround, traditional extracting method without
Method makes cell wall rupture, huge resistance to mass tranfer be present, causes extraction effect bad.Pass through certain physical means or chemical hand
Section causes qinghaosu leaf wall loose or ruptured, it will help qinghaosu therefrom dissolution can be effectively improved and carried by organic solvent
Efficiency is taken, the present invention has the advantages of preparation technology is simple, purification efficiency is higher, preparation cost is relatively low in a word.
Claims (1)
- A kind of 1. method of enzymolysis and extraction qinghaosu, it is characterised in that：Comprise the following steps：1）Stock, after artemisia leaf is crushed, add dilution heat of sulfuric acid or dilute hydrochloric acid solution that 21 times of weight pH value are 1, the aqueous solution With a certain amount of enzyme preparation, it is 61 DEG C, heated at constant temperature 25min to control temperature, is cooled down, filtering, in filtrate ether or petroleum ether A kind of organic solvent extract repeatedly, it is standby after filter cake low temperature drying；2）Heating, the organic solvent of 110 times of weight is added toward above-mentioned filter cake, control temperature as 61 DEG C, heated at constant temperature 25min is cold But, filter, filter cake is washed repeatedly with organic solvent, and filter cake discards, and filtrate and cleaning solution merge；3）Merge, combining step 1）With step 2）Gained extract, cleaning solution and filtrate, carried out with 2% sodium hydroxide solution Washing, is washed to neutrality with distillation, is concentrated under reduced pressure in 50 DEG C, obtain qinghaosu crude product, crude product passes through ethyl alcohol recrystallization, obtains qinghaosu Highly finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711415169.3A CN107857770A (en) | 2017-12-25 | 2017-12-25 | A kind of method of enzymolysis and extraction qinghaosu |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711415169.3A CN107857770A (en) | 2017-12-25 | 2017-12-25 | A kind of method of enzymolysis and extraction qinghaosu |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107857770A true CN107857770A (en) | 2018-03-30 |
Family
ID=61707408
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711415169.3A Pending CN107857770A (en) | 2017-12-25 | 2017-12-25 | A kind of method of enzymolysis and extraction qinghaosu |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107857770A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103304576A (en) * | 2012-03-07 | 2013-09-18 | 中国人民解放军第三军医大学 | Method for extracting artemisinin through enzymatic hydrolysis |
-
2017
- 2017-12-25 CN CN201711415169.3A patent/CN107857770A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103304576A (en) * | 2012-03-07 | 2013-09-18 | 中国人民解放军第三军医大学 | Method for extracting artemisinin through enzymatic hydrolysis |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108752231B (en) | Method for extracting theanine from sweet tea and simultaneously extracting rubusoside and tea polyphenol | |
| CN101829164B (en) | Biological preparation method of Hypericum perforatum L extractive | |
| CN104666427A (en) | Method for extracting main active components of folium mori | |
| CN103304576A (en) | Method for extracting artemisinin through enzymatic hydrolysis | |
| CN105287677A (en) | Method for extracting and further processing ainsliaea fragrans total flavonoid | |
| CN102617469A (en) | Method for extracting huperzine a from huperzia serrata | |
| CN101143887B (en) | Method for separating and preparing corosolicacid in loquat leaf | |
| CN107955017A (en) | A kind of qinghaosu Ultrasonic reflux extraction method | |
| CN107312015A (en) | A kind of technique of efficiently extracting and producing artemisinin by ionic liquid | |
| CN104926719B (en) | A kind of method that trigonelline is extracted from fructus cannabis | |
| US10968470B2 (en) | Method for preparing rubusoside | |
| CN104650173A (en) | Preparation method of tenuifolin through extraction from polygala tenuifolia | |
| CN107235988A (en) | A kind of extracting method of qinghaosu and Artemisitene | |
| CN107857770A (en) | A kind of method of enzymolysis and extraction qinghaosu | |
| CN103655655A (en) | Method for separating active ingredients from medicinal material by membrane technology | |
| CN115043889B (en) | Method for extracting synephrine, hesperidin and naringin from seville orange flower | |
| CN101412724B (en) | Method for extracting bilobalide compound from ginkgo leaf | |
| CN101879208A (en) | Method for extracting total flavonoids from mung bean shell | |
| CN104974202B (en) | A kind of method of the extraction separation Vitexin xyloside from natural products | |
| CN107365317A (en) | A kind of preparation technology using organic matter extracting artemisine | |
| CN106632549B (en) | A kind of method of naringin in ionic liquid extract pomelo peel | |
| CN108929350A (en) | A method of it distills to extract from XINGNAOJING ZHUSHEYE and recycles Gardenoside in waste liquid | |
| CN107298688A (en) | A kind of purification process for extracting concentration qinghaosu | |
| CN107793430A (en) | A kind of method of purification of qinghaosu | |
| CN102241683A (en) | Extraction method for seneciphyllin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180330 |