CN107854609A - Red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition are used for the purposes for preparing the medicine for the treatment of depression - Google Patents

Red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition are used for the purposes for preparing the medicine for the treatment of depression Download PDF

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CN107854609A
CN107854609A CN201711373742.9A CN201711373742A CN107854609A CN 107854609 A CN107854609 A CN 107854609A CN 201711373742 A CN201711373742 A CN 201711373742A CN 107854609 A CN107854609 A CN 107854609A
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tuber
red ginseng
dwarf lilyturf
fruit
chinese magnoliavine
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乔博灵
黄丽萍
段佳林
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Northwest University
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Northwest University
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/57—Magnoliaceae (Magnolia family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/79—Schisandraceae (Schisandra family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8968—Ophiopogon (Lilyturf)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization

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Abstract

It is used for the purposes of medicine for preparing treatment depression present invention relates particularly to red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition, the red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition are made up of the Chinese medicine material of following parts by weight：2~10 parts of red ginseng, 3~15 parts of the tuber of dwarf lilyturf, 2~8 parts of the fruit of Chinese magnoliavine.There is the new application of notable therapeutic effect present invention finds tradition applied to QI invigorating arrest sweating, the red ginseng of nourishing Yin and promoting production of body fluid, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition for treating depression.Red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition have obvious therapeutic effect applied to treating depression, can reduce the usage amount of Western medicine or substitute western medicine, have no toxic side effect, suitable for clinical application.

Description

Red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition are used for the purposes for preparing the medicine for the treatment of depression
Technical field
The invention belongs to Chinese medicine composition new application technical field, and in particular to a kind of red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition For the purposes for the medicine for preparing treatment depression.
Background technology
Depression is a kind of phrenoblabia using lasting depressive mood as main clinical manifestation, and homicide rate is most high-precision Refreshing section's disease.With the rapid development of modern society, the operating pressure of people and spiritual demand are growing day by day, the morbidity of depression Rate also shows the trend increased year by year.Depression is most mainly based on the decline of depressive mood, retardation of thinking and bulesis Various somatizations, including sleep disordered, weak, loss of appetite, hypogona dism etc. also be present in case.Clinic is mainly shown as Depressed, interest lowers, pessimistic, retardation of thinking, lacks initiative, from crime, diet, sleep are poor, worry that oneself is suffered from for self-accusation Various diseases, feel whole body many places discomfort, and severe patient may occur in which suicidal thought and behavior.Depression turns into global disease at present In to the mankind cause the second important diseases seriously born, painful to caused by patient and its family members, the damage to caused by society Mistake be other diseases it is incomparable.If lacking corresponding treatment, depression can be aggravated further, and severe patient threatens oneself And autre vie.
Antidepressants are roughly divided into three classes：MAOI, tricyclic antidepressants and heterocyclic antidepressant. This kind of medicine has many kinds, only not antidepressant curative effect, also has certain curative effect to anxious, anancastia and phobia. But to the uncomfortable symptom curative effect unobvious of various bodies, there is also more adverse reaction：Insomnia, dizziness, have a headache, be orthostatic low Blood pressure, tendon hyperreflexia, tremble, be powerless, hidrosis, dry, the drowsiness, dysuria also having, impotence etc., some patients occur Cutaneous anaphylaxis.Serious side effect may also occur is：Hypertensive crisis and toxic hepatic damage, so, it should take medicine Blood pressure and liver function are periodically checked and examined after preceding and medication.There are hepatitis history and cerebrovascular patient disabling, the elderly to use with caution.In addition, one A little effects are good, and the less new drug price of side effect costly, makes patient be difficult to bear.
Traditional Chinese medical science medication has integrally-regulated, and adverse reaction is few, can the advantage such as long-term use, more and more by the weight of people Depending on.But generally speaking, it is also immature to be used to treating depression for Chinese medicine or Chinese patent drug, market sale Chinese patent drug only have it is several, and Many is the compound formulation comprising plurality of Chinese, is worked slower, it is impossible to timely control symptom.Therefore, work very fast, security It is higher, the less medicament for treatment of depression of side effect, it can meet clinical needs.
The natural resources of Chinese medicinal materials in China enriches, and treatment by Chinese herbs is with a long history, existing many classical Chinese traditional patent formulations, application time compared with Long, curative for effect and security is preferable, and the new application of exploitation existing Chinese medicine composition has important researching value.
The content of the invention
Present invention finds red ginseng, the tuber of dwarf lilyturf and the five tastes sub-portfolio for being applied to treatment cardiovascular and cerebrovascular disease in traditional Chinese medicine The new application of thing, for the pharmaceutical composition only using three kinds of parts of generic medicinal plants as raw material, composition is simple, can be used by experimental verification In the medicine for preparing treatment depression, therapeutic effect is obvious and without bounce-back, reduces Western medicine toxic side effect, is answered suitable for clinical expansion With Chinese medicine preparation of the present invention.
It is used for the purposes of medicine for preparing treatment depression, institute the invention provides red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition Red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition is stated mainly to be made up of the Chinese medicine material of following parts by weight：2~10 parts of red ginseng, the tuber of dwarf lilyturf 3~15 Part, 2~8 parts of the fruit of Chinese magnoliavine.
Further, red ginseng of the present invention, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the medicine for preparing treatment depression Purposes, the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are made up of the Chinese medicine material of following parts by weight：2~4 parts of red ginseng, the tuber of dwarf lilyturf 6 ~10 parts, 3~7 parts of the fruit of Chinese magnoliavine.
Further, red ginseng of the present invention, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the medicine for preparing treatment depression Purposes, the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are made up of the Chinese medicine material of following parts by weight：2 parts of red ginseng, 6 parts of the tuber of dwarf lilyturf, 3 parts of the fruit of Chinese magnoliavine.
Further, red ginseng of the present invention, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the medicine for preparing treatment depression Purposes, the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition also include pharmaceutically acceptable excipient, diluent, auxiliary material, solvent Or combinations thereof.
Further, red ginseng of the present invention, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the medicine for preparing treatment depression Purposes, the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition for it is pharmaceutically acceptable be suitable to oral capsule, tablet, particle Agent, solution, powder, supensoid agent, or it is pharmaceutically acceptable injection suitable for intramuscular injection and/or intravenous injection, lyophilized Powder-injection.
Further, red ginseng of the present invention, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the medicine for preparing treatment depression Purposes, the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are pharmaceutically acceptable injection, and every milliliter of injection contains quite In 0.1~2.0g of primary dose red ginseng, 0.3~6.0g of the tuber of dwarf lilyturf, 0.15~3.0g of the fruit of Chinese magnoliavine；Every milliliter of injection contains panaxoside Rg1No less than 0.1mg；Or the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are pharmaceutically acceptable freeze drying powder injection.
Further, red ginseng of the present invention, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the medicine for preparing treatment depression Purposes, the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition injection are prepared according to following steps：
(i) red ginseng is ground into particle, and the ethanol for adding 60~100 times of volumes extracts 2~5 times, merging filtrate, is evaporated under reduced pressure Paste is concentrated into, adds the water for injection of 4~6 times of volumes, after being well mixed, filtration, filtrate is for matching somebody with somebody liquid；
(ii) tuber of dwarf lilyturf, the fruit of Chinese magnoliavine are extracted 2~5 times with the distilled water of 10~15 times of volumes, after extract solution concentration, with 80% second Decoction for the first time, 90% ethanol alcohol precipitation second and third time, is concentrated into paste by alcohol alcohol precipitation, then dry with vacuum drying, spraying Dry or freeze-drying is dried, and obtains the tuber of dwarf lilyturf, Schisandra chinens P.E, and the injection of 2~3 times of volumes of extract obtained addition is water-soluble Solution, filtering, filtrate is for matching somebody with somebody liquid；
(iii) above-mentioned red ginseng extract, the tuber of dwarf lilyturf and Fructus Schisandrae Chinensis extractive solution are uniformly mixed, adds appropriate excipient, then 0.5% activated carbon is added, is stirred 20~30 minutes, is filtered with the miillpore filter that aperture is 0.22 μm, filtrate adds water for injection, Adjust pH value to 4~9 with 20% sodium hydroxide, filtered with the miillpore filter that aperture is 0.22 μm produce injection red ginseng, the tuber of dwarf lilyturf and Fruit of Chinese magnoliavine composition.
Further, red ginseng of the present invention, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the medicine for preparing treatment depression Purposes, the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are pharmaceutically acceptable freeze drying powder injection, are made in accordance with the following methods It is standby：
(A) red ginseng is ground into particle, and the ethanol for adding 60~100 times of volumes extracts 2~5 times, merging filtrate, is evaporated under reduced pressure Paste is concentrated into, adds the water for injection of 4~6 times of volumes, after being well mixed, filtration, filtrate is for matching somebody with somebody liquid；
(B) tuber of dwarf lilyturf, the fruit of Chinese magnoliavine are extracted 2~5 times with the distilled water of 10~15 times of volumes, after extract solution concentration, with 80% second Decoction for the first time, 90% ethanol alcohol precipitation second and third time, is concentrated into paste by alcohol alcohol precipitation, then dry with vacuum drying, spraying Dry or freeze-drying is dried, and obtains the tuber of dwarf lilyturf, Schisandra chinens P.E, and the injection of 2~3 times of volumes of extract obtained addition is water-soluble Solution, filtering, filtrate is for matching somebody with somebody liquid；
(C) above-mentioned red ginseng extract, the tuber of dwarf lilyturf, Fructus Schisandrae Chinensis extractive solution are uniformly mixed, adds appropriate excipient, then add 0.5% activated carbon, stir 20~30 minutes, filtered with the miillpore filter that aperture is 0.22 μm, filtrate adds water for injection, uses 20% sodium hydroxide adjusts pH value to be filtered with the miillpore filter that aperture is 0.22 μm to 4~9 and produce red ginseng, the tuber of dwarf lilyturf and five tastes subgroup Compound parenteral solution；
(D) by the injection red ginseng obtained by step (C), the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composite injection according to every bottle of 5mL, After 10mL, 20mL quantitative separating, it is put into freeze drying box, in -25 DEG C~-50 DEG C of temperature after sterilizing, vacuum is 0.2~0.5Pa Under be freeze-dried, control the water content of freeze drying powder injection to seal produce rapidly 1%~5%.
Further, red ginseng of the present invention, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the medicine for preparing treatment depression Purposes, the excipient is mannitol, lactose, dextran, gelatin hydrolysate, glucose, sucrose or their mixture.
Further, red ginseng of the present invention, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the medicine for preparing treatment depression Purposes, the depression is that endogenous depression or/and reactive depression or/and masked depression or/and medicine draw The depression or/and suppression that the secondary depression risen or/and climacteric or post-natal depression or/and brain trauma or cerebral apoplexy induce The learning and memory of Secondary cases caused by strongly fragrant neurosis and/or depression declines or/and anhedonia.
Red ginseng, dry root after steaming of Araliaceae ginseng Panar ginseng C.A.Mey. cultivation product and Rhizome, red ginseng are the cooked food of ginseng, and red ginseng is warm-natured, sweet, slight bitter；Returns spleen, lung, the heart channel of Hang-Shaoyin and kidney channel, there is the multiple arteries and veins that reinforces vital energy Prevent prolapse, the effect of nourishing qi to stop, to be taken off for physically weak, cold weak pulse of limb, qi and blood is not taken the photograph, metrostaxis, belongs to what is classified under qi-restoratives medicine Qi-tonifying drug.Red ginseng is the good merchantable brand of insufficiency of vital energy and blood and inclined deficiency of yang person, all not warm often cold, trick, dizziness burnout, four occur Limb is weak, fatiguability, dyspnea with shortness of breath, when feel coldness in the stomach, long-term diarrhea, insomnia and dreamful sleep, the performance such as impotence frequent micturition, all can be selected Red ginseng is nourished.Body immunity can be improved by taking red ginseng, antifatigue, suppress tumour, adjust human endocrine system.The change of red ginseng Study point based on saponin(e and volatile oil, in addition also aliphatic acid, amino acid, sterol, flavone compound etc., modern pharmacology Research confirms that red ginseng Central nervous system has excitation, can reduce sense of fatigue, has to cardiac muscle and blood vessel and directly acts on, With antianaphylaxis shock and cardiotonic, also have the function that to improve anaemia.
The fruit of Chinese magnoliavine, also known as fructus schisandrae, Chinese magnoliavine, meeting and, five plums, Chinese prickly ash, be magnoliaceae schisandra Schisandrachinensis (Turcz.) Baill. dry mature fruit, it is warm-natured, it is sour, sweet.Return lung warp, the heart channel of Hang-Shaoyin, kidney Through.The effect of with astringent or styptic treatment for spontaneous sweating, nourishing generate fluid, kidney calming is restrained, the lung-intestine astringent medicinal under category astringent medicinal classification, for void of coughing long Asthma, emission, enuresis frequent micturition, endless diarrhea, spontaneous sweating, injury thirst, Heat Diabetes, palpitation and insomnia.The fruit of Chinese magnoliavine is containing more Kind lignanoid, also containing volatile oil, the influence to central stimulant pentylenetetrazole and strychnine median lethal dose.Benevolence contains schizandrin A, B, C, wuweizi alcohol A and wuweizi alcohol B.Separately containing schizandrin, gomisin A, gomisin D, gomisin E, GN, dagger-axe The pungent O of rice, go Radix Angelicae Sinensis acyl gomisin B, isoschizandrin, table Gomisin O, schisandra chinensis ester A, α-phellandrene, nerolidol, bar The compositions such as beans acyl Ge meter Xin.Pharmaceutical research confirms that the fruit of Chinese magnoliavine, which has, promotes body's immunity；Anti-oxidant and anti-aging；Shield Liver, induced liver drug metabolic enzyme；Kobadrin, it is antitumor；It is hypoglycemic；Cardiac stimulant；Strengthen organism adaptation ability；Antiulcer；It is anti- Nephropathy；The effect of antibacterial.
The tuber of dwarf lilyturf, alias：The tuber of dwarf lilyturf, very little winter, the very little tuber of dwarf lilyturf, the tuber of dwarf lilyturf are liliaceous plant tuber of dwarf lilyturf Ophiopogon japonicus (L.f.) KerGawl. dried root, there is the effect of nourishing Yin and promoting production of body fluid, moistening lung clears away heart-fire, for dryness of the lung dry cough.Tuberculosis cough, Tianjin Wound is thirsty, vexed insomnia, Heat Diabetes, dry constipation of intestines；Pharyngeal diphtheria.Main component ophiopogon saponin A, B, B ', C, C ', D, D ' (ophiopogoninA, B, B ', C, C ', D, D '), the tuber of dwarf lilyturf ketone A, B (ophiopogononeA, B), Methyl Ophiopogon flavonoid A, B (methy-lophiopogononeA, B), dihydro ophiopogonone A, B (ophiopogonanoneA, B) etc..Pharmacology confirmation, wheat Winter, which has, improves tolerance effect, has the function that cardiac stimulant protection cardiac muscle, arrhythmia, sedation.
Red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are traditionally applied to QI invigorating arrest sweating, the function of nourishing Yin and promoting production of body fluid, cure mainly thermal burn Vigour, the disease of the deficiency of both qi and yin of yin-fluid loss,《Chinese Pharmacopoeia》In the Shengmai Yin included be widely used in treating the heart, the cerebrovascular Disease (coronary heart diseases and angina pectoris, apoplexy, acute myocardial infarction, cerebral infarction) arrhythmia cordis, heart failure, vital myocarditis, lung Source property heart disease, essential hypotension etc..Shengmai Yin in wide clinical application for many years, it was demonstrated that safely and effectively.Further grinding In studying carefully, present invention finds the red ginseng necessarily matched, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition to have the effect of clear and definite to depression.
Red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are applied to treatment cardiovascular and cerebrovascular disease in traditional Chinese medicine, and Clinical practice is more Year, have no toxic side effect, it was demonstrated that its security.In further research, present invention finds the red ginseng necessarily matched, the tuber of dwarf lilyturf There is the effect of clear and definite to depression with fruit of Chinese magnoliavine composition.Red ginseng in the present invention, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composite formula be succinct, Preparation process is simple, verifies by clinic application, and its is evident in efficacy reliable, mild in medicine property and, can effectively improve Depression in Rats The various symptoms that model occurs, and inhibitory neurotransmitter taurine (Tau) and gamma aminobutyric acid in hippocampal tissue can be increased (GABA) concentration, the concentration of excitatory neurotransmitter aspartic acid (Asp) and glutamic acid (Glu) is reduced, it was demonstrated that of the invention is red Ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition play the role of to treat depression, that is, are used for the purposes for preparing the medicine for the treatment of depression.
, can basis when red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition of the present invention are used to prepare the medicine for the treatment of depression Need further to add pharmaceutically acceptable auxiliary material, as pharmaceutically acceptable carrier, excipient, diluent, auxiliary material, Solvent or combinations thereof.Selected auxiliary material is selected according to preparation general knowledge well known in the art, different formulations It is applicable different auxiliary materials.
The Chinese medicine composition of the present invention can be administered orally, drug administration by injection, Aerosol inhalation, local administration, per rectum Administration, nose administration, buccal administration.Term used herein " through injection " include it is subcutaneous, vein, it is intramuscular, close It is intrasternal in synovial membrane (chamber) in section, in film, intraocular, in liver, in focus, and injection or the infusion of encephalic Technology.Preferable composition is is administered orally, to Intraperitoneal medication or intravenous injection.The injection side of the composition sterile of the present invention Formula can be water or oil suspension.These suspension can be according to known technology using suitable dispersant, moistening Agent and suspending agent are manufactured by formula.Aseptic injection can be aseptic parenteral solution or suspension, be that injection is nontoxic acceptable Diluent or solvent, such as 1,3-BDO solution.These acceptable excipient and solvent can be water, Ringer's solution and isotonic Sodium chloride solution.Further, sterile non-volatile oil can be used as solvent or suspension media by convention.
The pharmaceutically acceptable red ginseng of the present invention, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition are preferably prepared to injection use.Injection Agent, as aseptic parenteral solution or oil suspension can use suitable dispersant, wetting agent and suspension according to known technology Agent is prepared by pharmaceutical formulation.Aseptic injection can be nontoxic through made of parenterally acceptable diluent or solvent Aseptic parenteral solution, suspension or emulsion, for example, 1,3-BDO solution.Acceptable excipient and solvent can be water, Lin Ge (family name) solution, U.S.P. and isotonic sodium chlorrde solution, it is preferable that excipient is bright using mannitol, lactose, dextran, hydrolysis Glue, glucose, sucrose or their mixture, to be optimal from mannitol.In addition, sterile non-volatile oil is according to used Example is used as solvent or suspension media.With this end in view any gentle non-volatile oil can include the list or two Portugal's bases of synthesis Diglyceride.In addition, aliphatic acid such as oleic acid can apply to injection.
Injection can be sterile, such as defend filter by bacterium and filter, or in the form of aseptic solid composite Bactericidal agent is mixed, can be dissolved in or be scattered in disinfectant or other sterile injectable mediums using preceding bactericidal agent.In order to prolong The effect of the compound of the long present invention, it usually needs the absorption of compound is slowed down by hypodermic injection or intramuscular injection.So It can realize solve the problems, such as crystal or non-crystalline material poorly water-soluble using liquid suspension.The absorptivity of compound depends on Its dissolution rate, successively depending on grain size and crystal shape.Furthermore it is possible to dissolved by compound in oil vehicles Or disperse to absorb to complete the delay of compound injection administration.
Injection storage form is to form chemical combination by biodegradable polymer, such as more lactic acid-polyglycolide What the microcapsule matrix of thing was completed.The controlled release ratio of compound depends on the ratio and particular polymer that compound forms polymer Property.Other biodegradable polymers include poly- (positive esters) and poly- (acid anhydrides).Injection storage form can also pass through Compound is embedded in the liposome compatible with bodily tissue or microemulsion is prepared.
The pharmaceutically acceptable red ginseng of the present invention, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition are further preferably prepared into powder-injection use.Powder pin The auxiliary material that agent can use has：Amino acid, glucose, lactose, sucrose, trehalose, mannitol, dextran, sterol (are consolidated containing courage Alcohol, diamino cholesterol, sojasterol, ergosterol, soybean steroid acyl sugar acid anhydride), sodium chloride, calcium gluconate or phosphate and mixed Compound.Phosphatide, including lecithin, soybean lecithin, cephalin, phosphate, DPPC, hydrogen phosphatidyl ethanol Ammonia, phosphatidylserine or its mixture.Cyclodextrin and its derivative, including beta-schardinger dextrin, 2-HP-BETA-CD, hydroxyl Second group-beta-cyclodextrin, 3- hydroxypropyl-β-cyclodextrins, methyl-B-cyclodextrin, sulfonic acid group-beta-cyclodextrin, glucosyl group-β-ring paste Essence or its mixture.Surfactant, including polyethylene glycol, Emulsifier EL-60, Crodaret, polyethylene Any several mixture of auxiliary material described in pyrrolidones, poloxamer or its mixture or above-mentioned a few classes.
With this end in view, any gentle non-volatile oil can be the list or diphenylglycerol diester of synthesis.Fat Acid, as oleic acid and its glyceride ester derivatives can be used for the preparation of injectable, as natural pharmaceutically acceptable oil Fat, such as olive oil or castor oil, particularly their polyoxyethylene deriv.These oil solutions or suspension can include long-chain Alcohol diluent or dispersant, such as carboxymethyl cellulose or similar dispersing agents, it is generally used for the medicine system of pharmaceutically acceptable formulation Agent includes emulsion and suspension.Other conventional surfactants, such as Tweens, spans and other emulsifying agents or biological medicament The hardening agent of efficiency, pharmaceutically acceptable solid, liquid, or other formulations are generally used for, and can apply to drug target The preparation of preparation.
The pharmaceutically acceptable composition of the present invention can be administered orally with any acceptable peroral dosage form, its In include, but is not limited to, capsule, tablet, powder, granule, water suspension or solution.It is administered orally, carries on tablet Body generally comprises lactose and cornstarch.Lubricant, such as magnesium stearate, are all typically added.It is administered, closes for capsule oral Suitable diluent includes lactose and dry cornstarch.When it is water suspension to be administered orally, its active ingredient is by emulsifying agent Formed with suspending agent.If expecting these formulations, some sweeteners, flavor enhancement or colouring agent can also be added.
The liquid dosage form of oral administration includes, but is not limited to, pharmaceutically acceptable emulsion, microemulsion, solution, suspends Liquid, syrup and elixir.In addition to the active compound, liquid dosage form can include known in general inert diluent, for example, water Or other solvents, solubilizer and emulsifying agent, such as ethanol, isopropanol, ethyl carbonate, ethyl acetate, phenmethylol, Ergol, Propane diols, 1,3-BDO, dimethylformamide, grease (particularly cottonseed, peanut, corn, microorganism, olive, castor-oil plant and Sesame oil), glycerine, Tetrahydrofurfuryl Alcohol, polyethylene glycol, sorbitan alcohol fatty acid ester, and their mixture.Except lazy Outside the diluent of property, Orally administered composition can also include assistant agent such as wetting agent, emulsifying agent or suspending agent, sweetener, flavor enhancement And aromatic.
The solid dosage forms of oral administration includes capsule, tablet, pill, pulvis and granula.In these formulations, active ingredient Thing mixes with least one pharmaceutically acceptable inert excipient or carrier, such as sodium citrate or calcium phosphate or filler or a) Filler such as starch, lactose, sucrose, glucose, mannitol and silicic acid, b) adhesive such as carboxymethyl cellulose, alginates are bright Glue, polyvinyl pyrrolidone, sucrose and Arabic gum, c) NMF such as glycerine, d) disintegrant such as agar, calcium carbonate, potato starch Or tapioca, alginic acid, some silicate and sodium carbonate, e) retardance agent solution such as paraffin, f) sorbefacient such as quaternary ammonium Compound, g) wetting agent such as hexadecanol and glycerin monostearate, h) absorbent such as white bole and bentonite, i) lubricant such as talcum Powder, calcium stearate, magnesium stearate, solid polyethylene glycol, Sodium Laurylsulfate, and their mixture.As for capsule, tablet and ball Agent, these formulations can include buffer.
Chinese medicine composition can be that filler riddles soft or hard capsule, and used auxiliary material has lactose and macromolecule Polyethylene glycol etc..Solid dosage forms photo agent, lozenge, capsule, pill and granula can pass through coating, shell adding such as enteric coating It is prepared with known coating method on other drugs preparation.They can optionally include opacifier, or preferably, in intestines Certain part in road, arbitrarily, with the sole active agent in the method release composition of delay.As implant compositions can wrap Containing multimeric species and wax.
Chinese medicine composition can form microcapsule formulations together with one or more excipient described in the invention.Solid The agent of formulation photo, lozenge, capsule, pill and granula can be by coating or shell addings, such as enteric coating, controlled release coat and other public affairs The drug formulation process known.In these solid dosage forms, reactive compound can mix with least one inert diluent, such as sugarcane Sugar, lactose or starch.Such formulation can also include additive besides inert diluents as in general application, such as Tableting lubricant and other compression aids such as magnesium stearate and microcrystalline cellulose.As for capsule, tablet and pill, these formulations can To include buffer.They can optionally include sedative, or preferably, certain in enteron aisle is a part of, with what is arbitrarily postponed Sole active agent in method release composition.Applicable implant compositions can include, but is not limited to, polymer and Wax.
The compound of the present invention is preferably prepared into dosage unit form to mitigate the equal of dosage and dosage by pharmaceutical formulation Even property.
, can basis when red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition of the present invention are used to prepare the medicine for the treatment of depression Need to be prepared into and pharmaceutically acceptable be suitable to oral capsule, tablet, granule, solution, powder, supensoid agent, or medicine Acceptable injection or freeze drying powder injection suitable for intramuscular injection and intravenous injection on, preferably injection or freeze-dried powder Agent.
Above-mentioned formulation can be prepared according to the conventional formulation method in this area.
Embodiment
In order that those of ordinary skill in the art more fully understand the present invention, this is expanded on further by the following examples Invention red ginseng, the composition of the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition, preparation method and the usage.But these embodiments are only limitted to illustrate this hair Bright rather than limitation the scope of the present invention.
Fluoxetine hydrochloride (20mg) used in following examples, is produced by Lilly Co., Eli., is matched somebody with somebody with deionized water Solution is made；Glutamic acid, aspartic acid, taurine and an aminobutyric acid are purchased from Sigma Co., USA.SD rats, male and female are simultaneous Have, between 180~220g of body weight, provided by Northwest University's school of life and health sciences Experimental Animal Center.Chinese medicine red ginseng, the tuber of dwarf lilyturf and five Taste purchase genunie medicinal materials, using preceding medicinal material red ginseng, the tuber of dwarf lilyturf and the fruit of Chinese magnoliavine respectively through choose, clean, dry, crush slabbing or Acinous is standby.
Red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are prepared into injection, freeze drying powder injection in research process, through pharmacology and Pharmacodynamic experiment proves that red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition of the present invention have antidepression effect.In embodiment prescription The ratio of each Chinese medicine weight proportion screens to obtain by science, the corresponding adjustment medicine group in the ratio range of the present invention The dosage of each medicinal material does not influence validity of the composition in treatment depression in compound.
(1) red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition prepare embodiment
Embodiment 1：Red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition
(1) injection
Red ginseng 50g is ground into particle, add 5L concentration be 80% ethanol decoct extraction 3 times, 2 hours every time, merging filtrate, Paste is concentrated under reduced pressure into, adds 200mL water for injection, after being well mixed, the miillpore filter that 0.4 μm of aperture filters, filtrate For with liquid.Tuber of dwarf lilyturf 150g, fruit of Chinese magnoliavine 75g distill water boiling and extraction 3 times with 2.5L, after extract solution concentration, with 80% ethanol Decoction for the first time, 90% ethanol alcohol precipitation second and third time, is concentrated into paste, then with being dried in vacuo, obtain by solution alcohol precipitation To the tuber of dwarf lilyturf, Schisandra chinens P.E, extract obtained dissolving, the filtering with microporous membrane that 0.4 μm of aperture, filtrate is for matching somebody with somebody liquid.Will be upper State red ginseng extract and the fruit of Chinese magnoliavine, Radix Ophiopogonis extract uniformly mix, after adding 50g mannitol, add 0.5% activated carbon, add Thermal agitation 20 minutes, after cooling, the miillpore filter that 0.22 μm of aperture filters, and clear and bright filtrate injects water to 500mL, with 20% Sodium hydroxide adjusts pH value to 7.5,0.22 μm of miillpore filters to filter, filling, and injection red ginseng, the tuber of dwarf lilyturf and the fruit of Chinese magnoliavine are produced after sterilizing Composition.
When pH is adjusted in above-mentioned preparation process, extract mixed liquor is all stable, wherein pH in the range of pH=4~9 =7.5 is optimal.The effect of excipient mannitol is to adjust the osmotic pressure of injection.Suitable excipient can also use lactose, Dextran, gelatin hydrolysate, glucose, sucrose or their mixture, to be optimal from mannitol.The addition of excipient 0.01~1.0g of excipient is added by final every milliliter of injection.As intravenous injection in use, compound concentration is higher, equally The water for injection that adds of crude drug amount it is few, the crude drug of above-mentioned same dosage, the intravenous injection that maximum concentration is configured to 25mL makes During with, drip-feed, compound concentration is relatively low, is typically configured to the use of 500mL injections.The concentration of injection is finally to obtain Every milliliter of parenteral solution contains main effective ingredient panaxoside Rg in injection1It is advisable no less than 0.1mg.Every milliliter of injection contains Equivalent to 0.1~2.0g of primary dose red ginseng, 0.3~6.0g of the tuber of dwarf lilyturf, 0.15~3.0g of the fruit of Chinese magnoliavine.
(2) freeze drying powder injection
After above-mentioned injection red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are dispensed by every bottle of 10mL, do not seal and be put into and freeze In the corresponding metal dish of case size, sterilization treatment is carried out to freeze drying box, and the temperature of its shelf is reduced to -30 DEG C, rapidly will Metal dish equipped with preparation is put into freeze drying box, is maintained 2 hours, is then carried out application of vacuum to freeze drying box, make the vacuum in case Spend for 0.2Pa, then shelf heated up, and be strict controlled in below -2 DEG C of eutectic point, kept for 12 hours, be finally warming up to 40 DEG C, And kept for certain time, formulation temperature is overlapped with shelf temperature, be put into sterile filtering gas, control the water content of preparation to exist 2%, seal produce injection red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composite freeze-dried powder agent rapidly.
Embodiment 2：Red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition
(1) injection red ginseng 41g is ground into particle, adds the ethanol that 2.16L concentration is 80% to decoct extraction 5 times, 2 is small every time When, merging filtrate, paste is concentrated under reduced pressure into, adds 180mL water for injection, after being well mixed, the micropore that 0.4 μm of aperture is filtered Membrane filtration mistake, filtrate is for matching somebody with somebody liquid.Tuber of dwarf lilyturf 62g, fruit of Chinese magnoliavine 166g are extracted 2 times with 3.5L distilled water, after extract solution concentration, with 80% Ethanol solution alcohol precipitation for the first time, 90% ethanol alcohol precipitation second and third time, decoction is concentrated into paste, then done with vacuum It is dry, the tuber of dwarf lilyturf, Schisandra chinens P.E are obtained, extract obtained dissolving, the filtering with microporous membrane that 0.4 μm of aperture, filtrate is for matching somebody with somebody liquid. Above-mentioned red ginseng extract and the fruit of Chinese magnoliavine, Radix Ophiopogonis extract are uniformly mixed, after adding 36g mannitol, add 0.5% activity Charcoal, heating stirring 30 minutes, after cooling, the miillpore filter that 0.22 μm of aperture filters, and clear and bright filtrate injects water to 500mL, Adjust pH value to 7.5 with 20% sodium hydroxide, the miillpore filter filtration that 0.22 μm of aperture is filling, produced after sterilizing injection red ginseng, The tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition.
(2) freeze drying powder injection
By above-mentioned injection red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition by every bottle of 5mL packing after, do not seal and be put into and freeze drying box In the corresponding metal dish of size, sterilization treatment is carried out to freeze drying box, and the temperature of its shelf is reduced to -30 DEG C, rapidly will dress The metal dish for having preparation is put into freeze drying box, is maintained 2 hours, is then carried out application of vacuum to freeze drying box, make the vacuum in case For 0.2Pa, then shelf heated up, and be strict controlled in below -2 DEG C of eutectic point, kept for 12 hours, be finally warming up to 40 DEG C, and Kept for certain time, formulation temperature is overlapped with shelf temperature, be put into sterile filtering gas, control the water content of preparation to exist 2%, rapid sealing.
Embodiment 3：Red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition
(1) injection red ginseng 80g is ground into particle, adds the ethanol that 6.4L concentration is 80% to decoct extraction 4 times, 2 is small every time When, merging filtrate, paste is concentrated under reduced pressure into, adds 400mL water for injection, after being well mixed, the micropore that 0.4 μm of aperture is filtered Membrane filtration mistake, filtrate is for matching somebody with somebody liquid.Tuber of dwarf lilyturf 120g, fruit of Chinese magnoliavine 64g are extracted 4 times with 2.5L distilled water, after extract solution concentration, with 80% Ethanol solution alcohol precipitation for the first time, 90% ethanol alcohol precipitation second and third time, decoction is concentrated into paste, then done with vacuum It is dry, the tuber of dwarf lilyturf, Schisandra chinens P.E are obtained, extract obtained dissolving, the filtering with microporous membrane that 0.4 μm of aperture, filtrate is for matching somebody with somebody liquid. Above-mentioned red ginseng extract and the fruit of Chinese magnoliavine, Radix Ophiopogonis extract are uniformly mixed, after adding 36g dextrans, add 0.5% activity Charcoal, heating stirring 30 minutes, after cooling, the miillpore filter that 0.22 μm of aperture filters, and clear and bright filtrate injects water to 500mL, Adjust pH value to 7.5 with 20% sodium hydroxide, the miillpore filter filtration that 0.22 μm of aperture is filling, produced after sterilizing injection red ginseng, The tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition.
(2) freeze drying powder injection
By above-mentioned injection red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition by every bottle of 5mL packing after, do not seal and be put into and freeze drying box In the corresponding metal dish of size, sterilization treatment is carried out to freeze drying box, and the temperature of its shelf is reduced to -30 DEG C, rapidly will dress The metal dish for having preparation is put into freeze drying box, is maintained 2 hours, is then carried out application of vacuum to freeze drying box, make the vacuum in case For 0.2Pa, then shelf heated up, and be strict controlled in below -2 DEG C of eutectic point, kept for 12 hours, be finally warming up to 40 DEG C, and Kept for certain time, formulation temperature is overlapped with shelf temperature, be put into sterile filtering gas, control the water content of preparation to exist 2%, rapid sealing.
Embodiment 4：Red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition
(1) injection red ginseng 52g is ground into particle, adds the ethanol that 5.2L concentration is 80% to decoct extraction 2 times, 2 is small every time When, merging filtrate, paste is concentrated under reduced pressure into, adds 300mL water for injection, after being well mixed, the micropore that 0.4 μm of aperture is filtered Membrane filtration mistake, filtrate is for matching somebody with somebody liquid.Tuber of dwarf lilyturf 130g, fruit of Chinese magnoliavine 91g are extracted 5 times with 2.2L distilled water, after extract solution concentration, with 80% Ethanol solution alcohol precipitation for the first time, 90% ethanol alcohol precipitation second and third time, decoction is concentrated into paste, then done with vacuum It is dry, the tuber of dwarf lilyturf, Schisandra chinens P.E are obtained, extract obtained dissolving, the filtering with microporous membrane that 0.4 μm of aperture, filtrate is for matching somebody with somebody liquid. Above-mentioned red ginseng extract and the fruit of Chinese magnoliavine, Radix Ophiopogonis extract are uniformly mixed, after adding 52g glucose, add 0.5% activity Charcoal, heating stirring 20 minutes, after cooling, the miillpore filter that 0.22 μm of aperture filters, and clear and bright filtrate injects water to 500mL, Adjusting pH value with 20% sodium hydroxide, the miillpore filter filtration of 0.22 μm of aperture is filling, and injection red ginseng, wheat are produced after sterilizing to 7.5 Winter and fruit of Chinese magnoliavine composition.
(2) freeze drying powder injection
After above-mentioned injection red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are dispensed by every bottle of 10mL, do not seal and be put into and freeze In the corresponding metal dish of case size, sterilization treatment is carried out to freeze drying box, and the temperature of its shelf is reduced to -30 DEG C, rapidly will Metal dish equipped with preparation is put into freeze drying box, is maintained 2 hours, is then carried out application of vacuum to freeze drying box, make the vacuum in case Spend for 0.2Pa, then shelf heated up, and be strict controlled in below -2 DEG C of eutectic point, kept for 12 hours, be finally warming up to 40 DEG C, And kept for certain time, formulation temperature is overlapped with shelf temperature, be put into sterile filtering gas, control the water content of preparation to exist 2%, rapid sealing.
Embodiment 5：Red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition
(1) injection red ginseng 48g is ground into particle, add 5L concentration be 80% ethanol decoct extraction 3 times, 2 hours every time, Merging filtrate, is concentrated under reduced pressure into paste, adds the water for injection of 250mL times of volume, after being well mixed, 0.4 μm of aperture it is micro- Hole filter membrane filtration, filtrate is for matching somebody with somebody liquid.Tuber of dwarf lilyturf 128g, fruit of Chinese magnoliavine 96g are extracted 3 times with 2.5L distilled water, after extract solution concentration, are used Decoction for the first time, 90% ethanol alcohol precipitation second and third time, is concentrated into paste by 80% ethanol solution alcohol precipitation, then with very Sky is dried, and obtains the tuber of dwarf lilyturf, Schisandra chinens P.E, extract obtained dissolving, the filtering with microporous membrane that 0.4 μm of aperture, filtrate is for matching somebody with somebody Liquid is used.Above-mentioned red ginseng extract and the fruit of Chinese magnoliavine, Radix Ophiopogonis extract are uniformly mixed, after adding 48g mannitol, add 0.5% Activated carbon, heating stirring 20 minutes, after cooling, the miillpore filter that 0.22 μm of aperture filters, and clear and bright filtrate injects water to 500mL, adjusting pH value with 20% sodium hydroxide, the miillpore filter filtration that 0.22 μm of aperture is filling, and injection is produced after sterilizing to 7.5 With red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition.
(2) freeze drying powder injection
After above-mentioned injection red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are dispensed by every bottle of 10mL, do not seal and be put into and freeze In the corresponding metal dish of case size, sterilization treatment is carried out to freeze drying box, and the temperature of its shelf is reduced to -30 DEG C, rapidly will Metal dish equipped with preparation is put into freeze drying box, is maintained 2 hours, is then carried out application of vacuum to freeze drying box, make the vacuum in case Spend for 0.2Pa, then shelf heated up, and be strict controlled in below -2 DEG C of eutectic point, kept for 12 hours, be finally warming up to 40 DEG C, And kept for certain time, formulation temperature is overlapped with shelf temperature, be put into sterile filtering gas, control the water content of preparation to exist 2%, rapid sealing.
Embodiment 6：Red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition
(1) injection red ginseng 50g is ground into particle, add 5L concentration be 80% ethanol decoct extraction 3 times, 2 hours every time, Merging filtrate, is concentrated under reduced pressure into paste, adds the water for injection of 300mL times of volume, after being well mixed, 0.4 μm of aperture it is micro- Hole filter membrane filtration, filtrate is for matching somebody with somebody liquid.Tuber of dwarf lilyturf 150g, fruit of Chinese magnoliavine 75g are extracted 3 times with 2.5L distilled water, after extract solution concentration, are used Decoction for the first time, 90% ethanol alcohol precipitation second and third time, is concentrated into paste by 80% ethanol solution alcohol precipitation, then with very Sky is dried, and obtains the tuber of dwarf lilyturf, Schisandra chinens P.E, extract obtained dissolving, filtering, filtrate is for matching somebody with somebody liquid.Above-mentioned red ginseng is extracted Liquid and the fruit of Chinese magnoliavine, Radix Ophiopogonis extract uniformly mix, add 50g lactose after, add 0.5% activated carbon, heating stirring 20 minutes, After cooling, the miillpore filter that 0.22 μm of aperture is filtered, and clear and bright filtrate injects water to 500mL, and pH is adjusted with 20% sodium hydroxide For value to 7.5, the miillpore filter filtration that 0.22 μm of aperture is filling, and injection red ginseng, the tuber of dwarf lilyturf and five tastes sub-portfolio are produced after sterilizing Thing.
(2) freeze drying powder injection
After above-mentioned injection red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are dispensed by every bottle of 10mL, do not seal and be put into and freeze In the corresponding metal dish of case size, sterilization treatment is carried out to freeze drying box, and the temperature of its shelf is reduced to -30 DEG C, rapidly will Metal dish equipped with preparation is put into freeze drying box, is maintained 2 hours, is then carried out application of vacuum to freeze drying box, make the vacuum in case Spend for 0.2Pa, then shelf heated up, and be strict controlled in below -2 DEG C of eutectic point, kept for 12 hours, be finally warming up to 40 DEG C, And kept for certain time, formulation temperature is overlapped with shelf temperature, be put into sterile filtering gas, control the water content of preparation to exist 2%, rapid sealing.
Except being prepared into the injection or freeze drying powder injection that are suitable to injection and use, red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition are used for Prepare treatment depression medicine when, can also be prepared into as needed it is pharmaceutically acceptable be suitable to oral capsule, tablet, Granule, solution, powder, supensoid agent.Red ginseng of the present invention, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition are used to preparing treatment depressed During the medicine of disease, pharmaceutically acceptable auxiliary material can be further added as needed, such as pharmaceutically acceptable excipient, Diluent, auxiliary material, solvent or combinations thereof.Above-mentioned formulation can be prepared into according to the conventional formulation method in this area Arrive.
By following pharmacological evaluation investigate embodiment 1~6 be prepared red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition injection, Freeze drying powder injection treats the effect of depression.
(2) pharmacological effect is tested
Experimental program：SD rats are randomly divided into blank control group (20), model group (20), extract with fluoxetine group (20,10mg/kg) and administration group (every group 20), stimulation is not awarded in blank control group whole experiment process, normal raising, Model group modeling but normal raising, administration group give red ginseng prepared by embodiment 1~6, the tuber of dwarf lilyturf, five from modeling daystart Taste sub-portfolio composition freeze-dried powder injection, dosage are 8g crude drugs/kg, are administered through tail vein injection, once a day, successive administration four Week.Extract with fluoxetine group gives Prozac from modeling daystart, gives medicament 10mg/kg, gastric infusion, once a day, connects Continuous administration surrounding.Modeling method, by the way that cortisone 40mg/kg is subcutaneously injected, 21 days induced rat depression models, pass through behavior Whether observation and open field test checking model succeed.
After model success, the state of mind, body weight, chaeta, pawl, ear color and luster, amount of drinking water and the activity feelings of each group rat are observed Condition, tested by spacious field, syrup is tested, measure amino acid content, therapeutic action of the evaluation composition to depression.
(1) syrup is tested：Before experiment in quiet room, animal training adapts to drinking-water containing sugar, places 2 simultaneously per cage Water bottle, the 1st 24 hours, 2 bottles be respectively arranged with mass concentration be 1% sucrose solution；Subsequent 24 hours, 1 bottled 1% sucrose is molten Liquid, 1 bottled pure water, 2 bottles of positions are random.Long-term chronic stress be preceding and stress terminate the same day, rat first 20 hours fasting for solids and liquids, so 2 water bottles are randomly placed to every rat afterwards, are respectively provided with 1% sucrose solution and pure water for claiming quality in advance.Rat is recorded 1 Syrup consumption, pure water consumption in hour.Calculate animal stress be front and rear syrup consumption and syrup preference (syrup is inclined Love=syrup consumption/total is liquid-consumed 100%).To above-mentioned blank control group, model group, extract with fluoxetine group and 6 groups of administration groups The syrup preference of rat calculates average respectively.Statistical procedures：
Experimental data is represented with mean+SD, with statistic software SPSS 18.0, one-way analysis of variance, LSD Method.Experimental result is as shown in table 1 below, wherein P<0.01 is to have notable significant difference.
The rat syrup consumption of table 1 and preference rate experimental result
##P<0.01vs blank control groups；**P<0.01vs model groups
Syrup consumption test embodies rat and the pleasant sensation of award is reacted.As shown in Table 1, each group rat syrup disappears before modeling Consumption and preference rate no difference of science of statistics；After model success, the syrup consumption and preference rate of model group rats are compared to blank Control group significantly reduces, and has notable significant difference (P with blank control group rat<0.01)；Give red ginseng, the tuber of dwarf lilyturf, the fruit of Chinese magnoliavine The administration group rat syrup consumption and preference rate of composition are significantly increased compared to model group, have significantly with model group rats Significant difference (P<0.01)；The administration group rat of the pharmaceutical composition of embodiment 1~6 is wherein given, syrup consumes after modeling Amount and preference rate and difference before modeling are smaller；Generally speaking syrup consumption and inclined after the modeling of administration group and extract with fluoxetine group Love rate is more or less the same, it was demonstrated that the red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition prepared by the embodiment of the present invention 1~6 are to Depression in Rats mould The therapeutic effect of type is suitable with antidepression clinical commonly used drug Prozac.
(2) rat open field test：Experiment spacious case apparatus used is made up of opaque timber, and bottom surface is 100cm × 100cm Square and be divided into 25 equilateral grids, around fill black paint vehicle for high 40cm wooden wall, field chamber interior wall.In experiment Preceding and experiment terminates rear afternoon 14：00~18：00 carries out this experimental observation in quiet room.The experiment of syrup preference terminates Afterwards, rat is put into the grid of center, observation rat passes through lattice number in 3min, and (4 pawls just count into grid, for horizontal fortune Dynamic score), rear number (2 fore paws soar or sought connections with wall, for the score that moves vertically).
To above-mentioned blank control group, model group, extract with fluoxetine group and the horizontal movement score of 6 groups of administration group rats and hang down Straight motion scores calculate average respectively.Statistical procedures：Experimental data is represented with mean+SD, uses statistical software SPSS18.0, one-way analysis of variance, LSD methods.Experimental result such as table 2 below shows, wherein P<0.01 is to have notable significant difference.
The rat open field test result of table 2
Group Horizontal movement Vertical movement
Blank control group 33.2±8.32 13.2±2.24
Model group 8.19±4.12## 5.72±2.35##
The administration group of embodiment 1 25.3±5.98** 13.1±2.69**
The administration group of embodiment 2 22.9±6.27** 11.7±2.19**
The administration group of embodiment 3 21.5±7.75** 10.9±2.96**
The administration group of embodiment 4 22.3±5.82** 11.3±2.28**
The administration group of embodiment 5 23.9±8.87** 12.3±2.94**
The administration group of embodiment 6 24.9±8.05** 12.6±2.65**
Extract with fluoxetine group 24.6±5.92** 11.8±2.87**
##P<0.01vs control groups；**P<0.01vs model groups.
Spacious field experiment be used to detecting rat starts in new environment, exploratory behaviour, and to the frightened state of anxiety and right The alertness of new environment；Horizontal movement reflects the situation of animal activity degree, curiosity of the vertical movement reflection animal to fresh ambient Degree, therefore it is usually used in the behavior evaluation of depression research.As shown in Table 2, after model success, the horizontal movement of model group rats Score and vertical movement score significantly reduce compared to blank control group, have notable significant difference (P with blank control group rat <0.01)；Red ginseng, the tuber of dwarf lilyturf, the administration group of fruit of Chinese magnoliavine composition are given compared with model group, administration group rat horizontal movement score (P is dramatically increased with vertical movement score<0.01), show to give Depression in Rats model red ginseng, the tuber of dwarf lilyturf, five tastes sub-portfolio Thing can improve the locomitivity of rat, and experimental result has statistically-significant difference.Generally speaking administration group and extract with fluoxetine The horizontal movement score and vertical movement score of group are more or less the same, it was demonstrated that red ginseng, the tuber of dwarf lilyturf prepared by the embodiment of the present invention 1~6, Fruit of Chinese magnoliavine composition is suitable with antidepression clinical commonly used drug Prozac to the therapeutic effect of Depression in Rats model.
(3) amino acid content in Rat hippocampus：After Behaviors survey terminates, broken end peels off brain, divides on ice bath From cortex of frontal lobe and hippocampus, weigh respectively, be put into 1.5mL cryopreservation tubes, be put into liquid nitrogen rapidly.Using conventional method, homogenate carries Take the amino acid in tissue.Using Shimadzu Lc-10ATVP series of high efficiency liquid chromatographs, with Lc.10ATVP high-pressure pumps and automatically Injector, RF.10AXL fluorescence detectors and Shimadzu chromatographic work station, detect the content of amino acid in tissue.Flow velocity 1mL/min； The μ L of sample size 20, fluorescence detector wavelength excitation wavelength are 357nm；Launch wavelength is 455nm.Statistical procedures：Experimental data Represented with mean+SD, with statistic software SPSS 18.0, one-way analysis of variance, LSD methods.
To Asp in above-mentioned blank control group, model group, extract with fluoxetine group and the hippocampal tissue of 6 groups of administration group rats and Glu concentration calculates average respectively.Experimental result such as table 3 below shows, wherein P<0.01 is to have notable significant difference.
Amino acid content experimental result in the rat hippocampus of table 3
Group Asp(μmol/g) Glu(μmol/g) Tau(μmol/g) GABA(μmol/g)
Blank control group 18.43±3.41 2.37±0.28 5.17±0.72 20.14±3.17
Model group 38.51±5.92## 5.69±0.31## 2.37±0.46## 11.33±4.15##
The administration group of embodiment 1 21.52±5.03** 3.05±0.49** 4.39±0.36** 18.68±4.56**
The administration group of embodiment 2 23.62±4.18** 3.78±0.37** 4.12±0.78** 16.76±4.26**
The administration group of embodiment 3 23.92±4.18** 3.51±0.42** 3.99±0.78** 16.92±3.94**
The administration group of embodiment 4 24.14±4.09** 3.44±0.36** 4.03±0.58** 17.03±4.53**
The administration group of embodiment 5 22.24±3.75** 3.15±0.49** 4.31±0.67** 18.57±4.65**
The administration group of embodiment 6 21.44±6.17** 3.17±0.48** 4.35±0.49** 17.96±4.37**
Extract with fluoxetine group 21.72±4.75** 3.14±0.49** 4.28±0.46** 17.98±4.26**
##P<0.01vs control groups；**P<0.01vs model groups.
Amino acid is the precursor substance of a variety of brain neurotransmitters as the indispensable a kind of material of human body, right Brain function and behavior have important adjustment effect.Glutamic acid (Glu) and aspartic acid (Asp) are mammalian central nervous systems Unite most important 2 kinds of endogenous excitatory amino acids.Gamma aminobutyric acid (GABA) and taurine (Tau) belong to inhibiting nerve and passed Matter, especially Tau, it is of a relatively high in intracerebral content, often play and act synergistically with GABA, play depression effect.
As shown in Table 3, Asp and Glu concentration significantly rises in model group rats hippocampal tissue, and Tau declines with GABA concentration, And there is significant difference (P with control group<0.01) red ginseng, the tuber of dwarf lilyturf, the administration group of fruit of Chinese magnoliavine composition and model group ratio, are given Compared with red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition administration group rat Asp, Glu concentration significantly reduce, and Tau and GABA concentration significantly raises (P<0.01).Generally speaking the Asp of administration group and extract with fluoxetine group, Glu concentration significantly reduce, and Tau and GABA concentration is notable Rise.
Experimental result confirms, red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition can alleviate rat chronic stress afterwards Glu, Asp in hippocampus Toxic action caused by accumulation in tissues, increase the level of the inhibitory aminoacid such as GABA and Tau in hippocampal tissue, reduce excited The ratio of property/inhibitory aminoacid.Red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition prepared by the embodiment of the present invention 1~6 press down to rat The therapeutic effect of strongly fragrant disease model is suitable with antidepression clinical commonly used drug Prozac.
In summary, red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition can effectively improve the various of Depression in Rats model appearance Symptom, and Tau and GABA concentration in hippocampal tissue can be increased, reduce Asp and Glu concentration, it was demonstrated that red ginseng, the tuber of dwarf lilyturf, five tastes subgroup Compound plays the role of to treat depression.The therapeutic effect and clinical conventional treatment of red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition to depression Agents fluoxetine is suitable.The usage amount of Western medicine can be reduced with Western medicine drug combination, reduces rebound phenomena, and prepared by the present invention Red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition have no toxic side effect, suitable for clinical application.
(3) clinical case：
(1) clinical trial protocol
100 patients are treated using traditional Chinese medicine powder injection of the present invention altogether, patient accepts for medical treatment from Neurology Clinic and in hospital Patients with depression；Wherein man 60, female 40；Age is 35~65 years old, average 51.4 years old, medical history 1~5 year.According to it is simple, At random, selected case is divided into treatment group and control group by the principle of parallel control, evaluates disease end of love before and after patient's medication, and right Than treatment group's (pharmaceutical composition of the present invention) and control group (Prozac) clinical efficacy difference.
(2) diagnostic criteria
Western medicine diagnostic criteria：General diagnostic criteria has an ICD-10 and DSM-IV in the world at present, domestic main to use
ICD-10, refer to the depression of First-episode and the depression of recurrence, not including Bipolar Depression.Patient generally has Mental state is low, interest and happy sense is lost, energy is not good or the classical symptom such as sense of fatigue.Other symptoms：1. focal attention and note The ability of meaning reduces；2. self-assessment reduces；3. from guilty idea and valueless sense (in mild episode also have)；4. think Prospects are dim is pessimistic；5. self inflicted injury or the idea committed suiside or behavior；6. sleep-disorder；7. loss of appetite.The course of disease continues at least 2 weeks.
Tcm diagnosis standard：With reference to《Chinese medical disease Standardization of diagnosis and curative effect》Strongly fragrant sick diagnostic criteria diagnosis basis 1. it is excellent it is strongly fragrant not Freely, lassitude, feeling of oppression over the chest and distention of hypochondrium, sighing frequently, or do not feel like eating, insomnia and dreamful sleep, irritability is kind the disease such as to cry；2. have worry too much, be grieved, sorrow The feelings will such as anxious hinders history；3. organic disease can be excluded through each systems inspection and laboratory examination；4. it should differentiate with insane sick, mad disease Disease diagnostic criteria；Main symptom：1. clear thread is not peaceful, 2. it is apt to think of and worries too much, is 3. apt to probably uneasiness；Secondary card：1. have a dizzy spell, pale complexion without China；2. spontaneous perspiration is powerless；3. insomnia and dreamful sleep；It is 4. uncomfortable in chest；5. palpitaition, shortness of breath；6. pale tongue, weak pulse；Main symptom is indispensable, secondary card 1.~6. have It is standby more than 1, it is diagnosable with reference to tongue fur, pulse condition.
(3) treatment method and evaluation criterion：
Red ginseng, the tuber of dwarf lilyturf prepared by treatment group's injection embodiment of the present invention 1, fruit of Chinese magnoliavine composite freeze-dried powder agent, every time 5.2g, after 500mL normal saline dilutions, intravenous injection administration, one time a day, 2 weeks are 1 course for the treatment of, and 3 course for the treatment of evaluations are treated As a result.
Control group：With Fluoxetine in Treatment, 2 pieces/day (40mg), evening sleep before take, the course for the treatment of is 3 weeks.
The standard of curative effect evaluation：Recovery from illness：Above-mentioned primary symptom disappears；It is effective：Above-mentioned primary symptom disappears substantially；Effectively：Above-mentioned primary symptom changes It is kind；It is invalid：Above-mentioned primary symptom is not improved.
(4) treatment results
The course for the treatment of result of patient 3 for the treatment of group 50 is fully recovered 32, effective 8, effective 7, invalid 3, total effective rate 94%.
Control group：The course for the treatment of result of patient 4 for the treatment of group 50 is fully recovered 28, effective 6, effective 13, invalid 3, always Effective percentage 94%.
Above clinical experiments have proved that the red ginseng of the present invention, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition are for when treating depression, always having Efficiency is suitable with Prozac, but cure rate is higher than Prozac, and overall treatment effect is better than Prozac.
Above content is to combine specific preferred embodiment further description made for the present invention, it is impossible to is assert The specific implementation of the present invention is confined to these explanations.For general technical staff of the technical field of the invention, On the premise of not departing from present inventive concept, some simple deduction or replace can also be made, specifically, in the medicine of the present invention Less adjustment is made on the basis of the prescription proportioning of composition, is imitated as long as no the treatment for fundamentally changing the present invention Fruit, it should all be considered as belonging to protection scope of the present invention.

Claims (10)

1. red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the purposes for preparing the medicine for the treatment of depression, it is characterised in that described red Ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are mainly made up of the Chinese medicine material of following parts by weight：2~10 parts of red ginseng, 3~15 parts of the tuber of dwarf lilyturf, 2~8 parts of the fruit of Chinese magnoliavine.
2. red ginseng according to claim 1, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the use for preparing the medicine for the treatment of depression On the way, it is characterised in that the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are made up of the Chinese medicine material of following parts by weight：Red ginseng 2~4 Part, 6~10 parts of the tuber of dwarf lilyturf, 3~7 parts of the fruit of Chinese magnoliavine.
3. red ginseng according to claim 1, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the use for preparing the medicine for the treatment of depression On the way, it is characterised in that the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are made up of the Chinese medicine material of following parts by weight：2 parts of red ginseng, 6 parts of the tuber of dwarf lilyturf, 3 parts of the fruit of Chinese magnoliavine.
4. red ginseng according to claim 1, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the use for preparing the medicine for the treatment of depression On the way, it is characterised in that the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition also include pharmaceutically acceptable excipient, diluent, auxiliary Material, solvent or combinations thereof.
5. red ginseng according to claim 4, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the use for preparing the medicine for the treatment of depression On the way, it is characterised in that the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are suitable to oral capsule, piece to be pharmaceutically acceptable Agent, granule, solution, powder, supensoid agent, or the pharmaceutically acceptable injection for being suitable to intramuscular injection and/or intravenous injection Agent, freeze drying powder injection.
6. red ginseng according to claim 5, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the use for preparing the medicine for the treatment of depression On the way, it is characterised in that the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are pharmaceutically acceptable injection, every milliliter of injection Containing equivalent to 0.1~2.0g of primary dose red ginseng, 0.3~6.0g of the tuber of dwarf lilyturf, 0.15~3.0g of the fruit of Chinese magnoliavine；Every milliliter of injection contains Panaxoside Rg1No less than 0.1mg；Or the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are pharmaceutically acceptable freeze-dried powder Injection.
7. red ginseng according to claim 6, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the use for preparing the medicine for the treatment of depression On the way, it is characterised in that the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition injection are prepared according to following steps：
(i) red ginseng is ground into particle, and the ethanol for adding 60~100 times of volumes extracts 2~5 times, merging filtrate, is concentrated under reduced pressure into thick Paste, the water for injection of 4~6 times of volumes is added, after being well mixed, filtration, filtrate is for matching somebody with somebody liquid；
(ii) tuber of dwarf lilyturf, the fruit of Chinese magnoliavine are extracted 2~5 times with the distilled water of 10~15 times of volumes, after extract solution concentration, with 80% ethanol alcohol It is heavy 90% ethanol alcohol precipitation second and third time, decoction to be concentrated into paste for the first time, then with vacuum drying, be spray-dried or Freeze-drying is dried, and obtains the tuber of dwarf lilyturf, Schisandra chinens P.E, and the extract obtained water for injection for adding 2~3 times of volumes dissolves, Filtering, filtrate is for matching somebody with somebody liquid；
(iii) above-mentioned red ginseng extract, the tuber of dwarf lilyturf and Fructus Schisandrae Chinensis extractive solution are uniformly mixed, adds appropriate excipient, then add 0.5% activated carbon, stir 20~30 minutes, filtered with the miillpore filter that aperture is 0.22 μm, filtrate adds water for injection, uses 20% sodium hydroxide adjusts pH value to be filtered with aperture for 0.22 μm of miillpore filter to 4~9 and produce injection red ginseng, the tuber of dwarf lilyturf and the five tastes Subgroup compound.
8. red ginseng according to claim 6, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the use for preparing the medicine for the treatment of depression On the way, it is characterised in that the red ginseng, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are pharmaceutically acceptable freeze drying powder injection, according to following It is prepared by method：
(A) red ginseng is ground into particle, and the ethanol for adding 60~100 times of volumes extracts 2~5 times, merging filtrate, is evaporated under reduced pressure concentration To paste, the water for injection of 4~6 times of volumes is added, after being well mixed, filtration, filtrate is for matching somebody with somebody liquid；
(B) tuber of dwarf lilyturf, the fruit of Chinese magnoliavine are extracted 2~5 times with the distilled water of 10~15 times of volumes, after extract solution concentration, with 80% ethanol alcohol It is heavy 90% ethanol alcohol precipitation second and third time, decoction to be concentrated into paste for the first time, then with vacuum drying, be spray-dried or Freeze-drying is dried, and obtains the tuber of dwarf lilyturf, Schisandra chinens P.E, and the extract obtained water for injection for adding 2~3 times of volumes dissolves, Filtering, filtrate is for matching somebody with somebody liquid；
(C) by above-mentioned red ginseng extract, the tuber of dwarf lilyturf, Fructus Schisandrae Chinensis extractive solution uniformly mix, add appropriate excipient, then add 0.5% activated carbon, stir 20~30 minutes, filtered with the miillpore filter that aperture is 0.22 μm, filtrate adds water for injection, uses hydrogen Aqueous solution of sodium oxide adjusts pH value to be filtered with aperture for 0.22 μm of miillpore filter to 4~9 and produce red ginseng, the tuber of dwarf lilyturf and five tastes sub-portfolio Composition injection；
(D) by the injection red ginseng obtained by step (C), the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composite injection according to every bottle of 5mL, 10mL, After 20mL quantitative separatings, it is put into freeze drying box, in -25 DEG C~-50 DEG C of temperature after sterilizing, vacuum is to be carried out under 0.2~0.5Pa Freeze-drying, the water content of freeze drying powder injection is controlled to seal produce rapidly 1%~5%.
9. red ginseng according to claim 8, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the use for preparing the medicine for the treatment of depression On the way, it is characterised in that the excipient is mannitol, lactose, dextran, gelatin hydrolysate, glucose, sucrose or theirs is mixed Compound.
10. red ginseng according to claim 1, the tuber of dwarf lilyturf and fruit of Chinese magnoliavine composition are used for the use for preparing the medicine for the treatment of depression On the way, it is characterised in that the depression be endogenous depression or/and reactive depression or/and masked depression or/and The depression that drug-induced secondary depression or/and climacteric or post-natal depression or/and brain trauma or cerebral apoplexy induce Or/and the learning and memory of Secondary cases caused by depressive neurosis and/or depression declines or/and anhedonia.
CN201711373742.9A 2017-12-19 2017-12-19 Red ginseng, the tuber of dwarf lilyturf, fruit of Chinese magnoliavine composition are used for the purposes for preparing the medicine for the treatment of depression Pending CN107854609A (en)

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* Cited by examiner, † Cited by third party
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CN110464008A (en) * 2018-05-10 2019-11-19 无限极（中国）有限公司 It is a kind of to have effects that depressurize Chinese medicine composition of relieving restlessness and preparation method thereof and purposes
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