CN107849607B - 血浆dna的单分子测序 - Google Patents

血浆dna的单分子测序 Download PDF

Info

Publication number
CN107849607B
CN107849607B CN201680046621.0A CN201680046621A CN107849607B CN 107849607 B CN107849607 B CN 107849607B CN 201680046621 A CN201680046621 A CN 201680046621A CN 107849607 B CN107849607 B CN 107849607B
Authority
CN
China
Prior art keywords
dna fragments
sequence
dna
cell
nanopore
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201680046621.0A
Other languages
English (en)
Chinese (zh)
Other versions
CN107849607A (zh
Inventor
卢煜明
赵慧君
郑淑恒
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chinese University of Hong Kong CUHK
Original Assignee
Chinese University of Hong Kong CUHK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chinese University of Hong Kong CUHK filed Critical Chinese University of Hong Kong CUHK
Priority to CN202211147693.8A priority Critical patent/CN115433769A/zh
Publication of CN107849607A publication Critical patent/CN107849607A/zh
Application granted granted Critical
Publication of CN107849607B publication Critical patent/CN107849607B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/197—Modifications characterised by incorporating a spacer/coupling moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/146—Concentration of target or template
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Nanotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
CN201680046621.0A 2015-08-12 2016-08-12 血浆dna的单分子测序 Active CN107849607B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202211147693.8A CN115433769A (zh) 2015-08-12 2016-08-12 血浆dna的单分子测序

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562204396P 2015-08-12 2015-08-12
US62/204,396 2015-08-12
PCT/CN2016/094802 WO2017025059A1 (en) 2015-08-12 2016-08-12 Single-molecule sequencing of plasma dna

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202211147693.8A Division CN115433769A (zh) 2015-08-12 2016-08-12 血浆dna的单分子测序

Publications (2)

Publication Number Publication Date
CN107849607A CN107849607A (zh) 2018-03-27
CN107849607B true CN107849607B (zh) 2022-10-11

Family

ID=57983895

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201680046621.0A Active CN107849607B (zh) 2015-08-12 2016-08-12 血浆dna的单分子测序
CN202211147693.8A Pending CN115433769A (zh) 2015-08-12 2016-08-12 血浆dna的单分子测序

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202211147693.8A Pending CN115433769A (zh) 2015-08-12 2016-08-12 血浆dna的单分子测序

Country Status (12)

Country Link
US (2) US11319586B2 (https=)
EP (1) EP3334843A4 (https=)
JP (3) JP6867045B2 (https=)
CN (2) CN107849607B (https=)
AU (3) AU2016305103C1 (https=)
CA (1) CA2995422A1 (https=)
HK (1) HK1248286A1 (https=)
IL (3) IL285795B (https=)
MY (1) MY188348A (https=)
SA (1) SA518390907B1 (https=)
TW (2) TWI728994B (https=)
WO (1) WO2017025059A1 (https=)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019040546A1 (en) 2017-08-23 2019-02-28 Roche Sequencing Solutions, Inc ENZYME SCREENING METHODS
US20210062256A1 (en) * 2017-09-07 2021-03-04 Coopergenomics, Inc. Systems and methods for non-invasive preimplantation genetic diagnosis
EP3700856A4 (en) 2017-10-26 2021-12-15 Ultima Genomics, Inc. METHODS AND SYSTEMS FOR SEQUENCE CALL
EP3731959B1 (en) 2017-12-29 2025-10-08 Clear Labs, Inc. Automated priming and library loading device
WO2019204720A1 (en) * 2018-04-20 2019-10-24 The Regents Of The University Of California Nucleic acid sequencing methods and computer-readable media for practicing same
WO2020185790A1 (en) 2019-03-10 2020-09-17 Ultima Genomics, Inc. Methods and systems for sequence calling
US12205674B2 (en) 2019-06-21 2025-01-21 Coopersurgical, Inc. System and method for determining genetic relationships between a sperm provider, oocyte provider, and the respective conceptus
WO2021108532A2 (en) * 2019-11-25 2021-06-03 William Marsh Rice University Linear dna assembly for nanopore sequencing
WO2022008641A1 (en) 2020-07-08 2022-01-13 Roche Sequencing Solutions, Inc. Split-pool synthesis apparatus and methods of performing split-pool synthesis
CN116710571A (zh) 2020-10-15 2023-09-05 卡帕生物系统公司 用于下一代测序文库制备的电泳装置和方法
CN114507708A (zh) * 2020-11-16 2022-05-17 唐纳德·李 一种制备长分子测序dna的方法
EP4308723B1 (en) 2021-03-15 2025-04-23 F. Hoffmann-La Roche AG Targeted next-generation sequencing via anchored primer extension
CN117098854A (zh) 2021-03-26 2023-11-21 豪夫迈·罗氏有限公司 杂交缓冲液配制品
WO2022207682A1 (en) 2021-04-01 2022-10-06 F. Hoffmann-La Roche Ag Immune cell counting of sars-cov-2 patients based on immune repertoire sequencing
US20220328135A1 (en) * 2021-04-12 2022-10-13 The Chinese University Of Hong Kong Base modification analysis using electrical signals
WO2023023561A1 (en) * 2021-08-17 2023-02-23 Quantapore, Inc. Multiplex binding assays with optical nanopore readout
US20250243536A1 (en) * 2021-10-18 2025-07-31 Belgian Volition Srl Use of nanopore sequencing for determining the origin of circulating dna
EP4547839A1 (en) 2022-06-30 2025-05-07 Kapa Biosystems, Inc. Controlling for tagmentation sequencing library insert size using archaeal histone-like proteins
CN114898802B (zh) * 2022-07-14 2022-09-30 臻和(北京)生物科技有限公司 基于血浆游离dna甲基化测序数据的末端序列频率分布特征确定方法、评价方法及装置
EP4581153A1 (en) 2022-09-02 2025-07-09 F. Hoffmann-La Roche AG Improvements to next-generation target enrichment performance
WO2024215528A1 (en) 2023-04-13 2024-10-17 Ventana Medical Systems, Inc. Proliferation assay for fixed solid tumors
CN121605113A (zh) 2023-06-14 2026-03-03 豪夫迈·罗氏有限公司 5-甲酰基胞嘧啶的加速标记及在核酸甲基化测序中的应用
WO2025193604A1 (en) 2024-03-11 2025-09-18 Ventana Medical Systems, Inc. Non-enzymatic dissociation of ffpe tissue and generation of single cells with intact cell surface markers
WO2025210056A1 (en) 2024-04-03 2025-10-09 Roche Sequencing Solutions, Inc. In vitro amplification of dna methylation patterns
WO2025247632A1 (en) 2024-05-27 2025-12-04 European Molecular Biology Laboratory Preparation of cell-free fragmented nucleic acids for genetic analysis sequencing
WO2026082489A1 (en) 2024-10-18 2026-04-23 F. Hoffmann-La Roche Ag Universal pcr to boost molecule recovery in cell free dna library preparation and target enrichment workflows

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012060595A2 (en) * 2010-11-01 2012-05-10 Lg Electronics Inc. Structure with nanopore and apparatus for determining sequences of nucleic acids including the same
CN103827320A (zh) * 2011-07-25 2014-05-28 牛津纳米孔技术有限公司 使用跨膜孔进行双链多核苷酸测序的发夹环方法
WO2016077313A1 (en) * 2014-11-11 2016-05-19 Bgi Shenzhen Multi-pass sequencing

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
GB0504774D0 (en) * 2005-03-08 2005-04-13 Lingvitae As Method
JP2008161056A (ja) 2005-04-08 2008-07-17 Hiroaki Mita Ｄｎａ配列解析装置、ｄｎａ配列解析方法およびプログラム
PL2557520T3 (pl) 2007-07-23 2021-10-11 The Chinese University Of Hong Kong Określanie zaburzenia równowagi sekwencji kwasu nukleinowego
AU2009325069B2 (en) 2008-12-11 2015-03-19 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
DE102008061772A1 (de) * 2008-12-11 2010-06-17 Febit Holding Gmbh Verfahren zur Untersuchung von Nukleinsäure-Populationen
EP3783110B1 (en) 2009-11-05 2022-11-23 The Chinese University Of Hong Kong Fetal genomic analysis from a maternal biological sample
CA2780016C (en) 2009-11-06 2017-09-19 The Chinese University Of Hong Kong Size-based genomic analysis
US9121823B2 (en) 2010-02-19 2015-09-01 The Trustees Of The University Of Pennsylvania High-resolution analysis devices and related methods
CN105243295B (zh) 2010-11-30 2018-08-17 香港中文大学 与癌症相关的遗传或分子畸变的检测
AU2011373694A1 (en) * 2011-07-26 2013-05-02 Verinata Health, Inc. Method for determining the presence or absence of different aneuploidies in a sample
US20130040827A1 (en) 2011-08-14 2013-02-14 Stephen C. Macevicz Method and compositions for detecting and sequencing nucleic acids
CN107828877A (zh) 2012-01-20 2018-03-23 吉尼亚科技公司 基于纳米孔的分子检测与测序
GB2559073A (en) 2012-06-08 2018-07-25 Pacific Biosciences California Inc Modified base detection with nanopore sequencing
WO2013188841A1 (en) * 2012-06-15 2013-12-19 Genia Technologies, Inc. Chip set-up and high-accuracy nucleic acid sequencing
US11261494B2 (en) 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
GB201215449D0 (en) 2012-08-30 2012-10-17 Zoragen Biotechnologies Llp Method of detecting chromosonal abnormalities
SI4056712T1 (sl) 2012-09-20 2024-10-30 The Chinese University Of Hong Kong Neinvazivno določanje metiloma tumorja iz plazme
US10767222B2 (en) * 2013-12-11 2020-09-08 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
AU2016406443A1 (en) 2016-05-10 2018-11-15 Nec Corporation Demand and supply adjustment system, control apparatus, control method, and program

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012060595A2 (en) * 2010-11-01 2012-05-10 Lg Electronics Inc. Structure with nanopore and apparatus for determining sequences of nucleic acids including the same
CN103827320A (zh) * 2011-07-25 2014-05-28 牛津纳米孔技术有限公司 使用跨膜孔进行双链多核苷酸测序的发夹环方法
WO2016077313A1 (en) * 2014-11-11 2016-05-19 Bgi Shenzhen Multi-pass sequencing
CN107002130A (zh) * 2014-11-11 2017-08-01 深圳华大基因研究院 多程测序

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Nannan Liu等.Two-Way Nanopore Sensing of Sequence-Specific Oligonucleotides and Small-Molecule Targets in Complex Matrices Using Integrated DNA Supersandwich Structures.《Angew. Chem. Int.Ed.》.2013,第52卷 *
SWAN: sliding window analysis of nucleotide sequence variability;Vitali Proutski等;《Bioinformatics》;19981231;第14卷(第5期);第467页第1-2段 *
Two-Way Nanopore Sensing of Sequence-Specific Oligonucleotides and Small-Molecule Targets in Complex Matrices Using Integrated DNA Supersandwich Structures;Nannan Liu等;《Angew. Chem. Int.Ed.》;20131231;第52卷;第2007页右栏第2段、第2008页左栏第2段、第2010页左栏第3段和图1 *
基于纳米孔在复杂基质中的核酸和小分子双检测;夏帆;《中国化学会第29届学术年会摘要集——第02分会：分离分析及微、纳流控新方法》;20140711;第2页第1段和图1 *

Also Published As

Publication number Publication date
JP7506408B2 (ja) 2024-06-26
TWI728994B (zh) 2021-06-01
IL285795A (en) 2021-09-30
CN107849607A (zh) 2018-03-27
TW202130815A (zh) 2021-08-16
AU2016305103A1 (en) 2018-03-22
MY188348A (en) 2021-12-02
US11319586B2 (en) 2022-05-03
TWI793586B (zh) 2023-02-21
IL257074B (en) 2021-09-30
AU2020202153A1 (en) 2020-04-16
JP2021118691A (ja) 2021-08-12
EP3334843A1 (en) 2018-06-20
TW201718874A (zh) 2017-06-01
AU2016305103B2 (en) 2020-01-02
JP2024113130A (ja) 2024-08-21
AU2023202572B2 (en) 2025-04-10
JP7810455B2 (ja) 2026-02-03
IL294192B1 (en) 2023-04-01
US20220205038A1 (en) 2022-06-30
IL294192B2 (en) 2023-08-01
WO2017025059A1 (en) 2017-02-16
SA518390907B1 (ar) 2021-09-08
EP3334843A4 (en) 2019-01-02
HK1245850A1 (zh) 2018-08-31
AU2023202572A1 (en) 2023-05-18
IL294192A (en) 2022-08-01
AU2020202153B2 (en) 2023-02-02
JP2018531583A (ja) 2018-11-01
AU2016305103C1 (en) 2020-06-04
IL285795B (en) 2022-07-01
US20170044606A1 (en) 2017-02-16
JP6867045B2 (ja) 2021-04-28
CA2995422A1 (en) 2017-02-16
CN115433769A (zh) 2022-12-06
IL257074A (en) 2018-03-29
HK1248286A1 (zh) 2018-10-12

Similar Documents

Publication Publication Date Title
JP7810455B2 (ja) 血漿ｄｎａの単分子配列決定
CN107077537B (zh) 用短读测序数据检测重复扩增
TWI867325B (zh) 利用血漿中dna的尺寸及數量畸變以偵測癌症
ES2564656T3 (es) Medios y métodos para el diagnóstico no invasivo de la aneuploidía cromosómica
TWI727938B (zh) 血漿粒線體ｄｎａ分析之應用
CN114999567A (zh) 游离dna的片段化模式的分析
CN113832139A (zh) 使用具有独特分子索引(umi)的冗余读段在测序dna片段中抑制误差
CN110023509A (zh) 基因型分型测定中的非独特条形码
HK40083172A (en) Single-molecule sequencing of plasma dna
HK1245850B (en) Single-molecule sequencing of plasma dna
HK40058694A (en) Detecting repeat expansions with short read sequencing data
HK40079331A (en) Analysis of fragmentation patterns of cell-free dna
HK1205204B (zh) 快速非整倍性检测
HK1237825A1 (en) Applications of plasma mitochondrial dna analysis

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1245850

Country of ref document: HK

GR01 Patent grant
GR01 Patent grant
TG01 Patent term adjustment
TG01 Patent term adjustment

Free format text: NEW EXPIRY DATE: 20361219