TWI728994B - 血漿dna之單分子定序 - Google Patents

血漿dna之單分子定序 Download PDF

Info

Publication number
TWI728994B
TWI728994B TW105125873A TW105125873A TWI728994B TW I728994 B TWI728994 B TW I728994B TW 105125873 A TW105125873 A TW 105125873A TW 105125873 A TW105125873 A TW 105125873A TW I728994 B TWI728994 B TW I728994B
Authority
TW
Taiwan
Prior art keywords
dna fragments
plasma
sequence
dna
serum
Prior art date
Application number
TW105125873A
Other languages
English (en)
Chinese (zh)
Other versions
TW201718874A (zh
Inventor
煜明 盧
慧君 趙
淑恒 鄭
Original Assignee
香港中文大學
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 香港中文大學 filed Critical 香港中文大學
Publication of TW201718874A publication Critical patent/TW201718874A/zh
Application granted granted Critical
Publication of TWI728994B publication Critical patent/TWI728994B/zh

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721Investigating individual macromolecules, e.g. by translocation through nanopores
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10Sequence alignment; Homology search
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/50Other enzymatic activities
    • C12Q2521/501Ligase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/151Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/191Modifications characterised by incorporating an adaptor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/197Modifications characterised by incorporating a spacer/coupling moiety
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/146Concentration of target or template
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60Detection means characterised by use of a special device
    • C12Q2565/631Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Nanotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
TW105125873A 2015-08-12 2016-08-12 血漿dna之單分子定序 TWI728994B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562204396P 2015-08-12 2015-08-12
US62/204,396 2015-08-12

Publications (2)

Publication Number Publication Date
TW201718874A TW201718874A (zh) 2017-06-01
TWI728994B true TWI728994B (zh) 2021-06-01

Family

ID=57983895

Family Applications (2)

Application Number Title Priority Date Filing Date
TW105125873A TWI728994B (zh) 2015-08-12 2016-08-12 血漿dna之單分子定序
TW110115619A TWI793586B (zh) 2015-08-12 2016-08-12 血漿dna之單分子定序

Family Applications After (1)

Application Number Title Priority Date Filing Date
TW110115619A TWI793586B (zh) 2015-08-12 2016-08-12 血漿dna之單分子定序

Country Status (12)

Country Link
US (2) US11319586B2 (https=)
EP (1) EP3334843A4 (https=)
JP (3) JP6867045B2 (https=)
CN (2) CN107849607B (https=)
AU (3) AU2016305103C1 (https=)
CA (1) CA2995422A1 (https=)
HK (1) HK1248286A1 (https=)
IL (3) IL285795B (https=)
MY (1) MY188348A (https=)
SA (1) SA518390907B1 (https=)
TW (2) TWI728994B (https=)
WO (1) WO2017025059A1 (https=)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019040546A1 (en) 2017-08-23 2019-02-28 Roche Sequencing Solutions, Inc ENZYME SCREENING METHODS
US20210062256A1 (en) * 2017-09-07 2021-03-04 Coopergenomics, Inc. Systems and methods for non-invasive preimplantation genetic diagnosis
EP3700856A4 (en) 2017-10-26 2021-12-15 Ultima Genomics, Inc. METHODS AND SYSTEMS FOR SEQUENCE CALL
EP3731959B1 (en) 2017-12-29 2025-10-08 Clear Labs, Inc. Automated priming and library loading device
WO2019204720A1 (en) * 2018-04-20 2019-10-24 The Regents Of The University Of California Nucleic acid sequencing methods and computer-readable media for practicing same
WO2020185790A1 (en) 2019-03-10 2020-09-17 Ultima Genomics, Inc. Methods and systems for sequence calling
US12205674B2 (en) 2019-06-21 2025-01-21 Coopersurgical, Inc. System and method for determining genetic relationships between a sperm provider, oocyte provider, and the respective conceptus
WO2021108532A2 (en) * 2019-11-25 2021-06-03 William Marsh Rice University Linear dna assembly for nanopore sequencing
WO2022008641A1 (en) 2020-07-08 2022-01-13 Roche Sequencing Solutions, Inc. Split-pool synthesis apparatus and methods of performing split-pool synthesis
CN116710571A (zh) 2020-10-15 2023-09-05 卡帕生物系统公司 用于下一代测序文库制备的电泳装置和方法
CN114507708A (zh) * 2020-11-16 2022-05-17 唐纳德·李 一种制备长分子测序dna的方法
EP4308723B1 (en) 2021-03-15 2025-04-23 F. Hoffmann-La Roche AG Targeted next-generation sequencing via anchored primer extension
CN117098854A (zh) 2021-03-26 2023-11-21 豪夫迈·罗氏有限公司 杂交缓冲液配制品
WO2022207682A1 (en) 2021-04-01 2022-10-06 F. Hoffmann-La Roche Ag Immune cell counting of sars-cov-2 patients based on immune repertoire sequencing
US20220328135A1 (en) * 2021-04-12 2022-10-13 The Chinese University Of Hong Kong Base modification analysis using electrical signals
WO2023023561A1 (en) * 2021-08-17 2023-02-23 Quantapore, Inc. Multiplex binding assays with optical nanopore readout
US20250243536A1 (en) * 2021-10-18 2025-07-31 Belgian Volition Srl Use of nanopore sequencing for determining the origin of circulating dna
EP4547839A1 (en) 2022-06-30 2025-05-07 Kapa Biosystems, Inc. Controlling for tagmentation sequencing library insert size using archaeal histone-like proteins
CN114898802B (zh) * 2022-07-14 2022-09-30 臻和(北京)生物科技有限公司 基于血浆游离dna甲基化测序数据的末端序列频率分布特征确定方法、评价方法及装置
EP4581153A1 (en) 2022-09-02 2025-07-09 F. Hoffmann-La Roche AG Improvements to next-generation target enrichment performance
WO2024215528A1 (en) 2023-04-13 2024-10-17 Ventana Medical Systems, Inc. Proliferation assay for fixed solid tumors
CN121605113A (zh) 2023-06-14 2026-03-03 豪夫迈·罗氏有限公司 5-甲酰基胞嘧啶的加速标记及在核酸甲基化测序中的应用
WO2025193604A1 (en) 2024-03-11 2025-09-18 Ventana Medical Systems, Inc. Non-enzymatic dissociation of ffpe tissue and generation of single cells with intact cell surface markers
WO2025210056A1 (en) 2024-04-03 2025-10-09 Roche Sequencing Solutions, Inc. In vitro amplification of dna methylation patterns
WO2025247632A1 (en) 2024-05-27 2025-12-04 European Molecular Biology Laboratory Preparation of cell-free fragmented nucleic acids for genetic analysis sequencing
WO2026082489A1 (en) 2024-10-18 2026-04-23 F. Hoffmann-La Roche Ag Universal pcr to boost molecule recovery in cell free dna library preparation and target enrichment workflows

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006095169A1 (en) * 2005-03-08 2006-09-14 Lingvitae As Method for preparing polynucleotides for analysis
US20120045771A1 (en) * 2008-12-11 2012-02-23 Febit Holding Gmbh Method for analysis of nucleic acid populations
WO2012060595A2 (en) * 2010-11-01 2012-05-10 Lg Electronics Inc. Structure with nanopore and apparatus for determining sequences of nucleic acids including the same
US20130040827A1 (en) * 2011-08-14 2013-02-14 Stephen C. Macevicz Method and compositions for detecting and sequencing nucleic acids

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
JP2008161056A (ja) 2005-04-08 2008-07-17 Hiroaki Mita Dna配列解析装置、dna配列解析方法およびプログラム
PL2557520T3 (pl) 2007-07-23 2021-10-11 The Chinese University Of Hong Kong Określanie zaburzenia równowagi sekwencji kwasu nukleinowego
AU2009325069B2 (en) 2008-12-11 2015-03-19 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
EP3783110B1 (en) 2009-11-05 2022-11-23 The Chinese University Of Hong Kong Fetal genomic analysis from a maternal biological sample
CA2780016C (en) 2009-11-06 2017-09-19 The Chinese University Of Hong Kong Size-based genomic analysis
US9121823B2 (en) 2010-02-19 2015-09-01 The Trustees Of The University Of Pennsylvania High-resolution analysis devices and related methods
CN105243295B (zh) 2010-11-30 2018-08-17 香港中文大学 与癌症相关的遗传或分子畸变的检测
JP6298404B2 (ja) * 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
AU2011373694A1 (en) * 2011-07-26 2013-05-02 Verinata Health, Inc. Method for determining the presence or absence of different aneuploidies in a sample
CN107828877A (zh) 2012-01-20 2018-03-23 吉尼亚科技公司 基于纳米孔的分子检测与测序
GB2559073A (en) 2012-06-08 2018-07-25 Pacific Biosciences California Inc Modified base detection with nanopore sequencing
WO2013188841A1 (en) * 2012-06-15 2013-12-19 Genia Technologies, Inc. Chip set-up and high-accuracy nucleic acid sequencing
US11261494B2 (en) 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
GB201215449D0 (en) 2012-08-30 2012-10-17 Zoragen Biotechnologies Llp Method of detecting chromosonal abnormalities
SI4056712T1 (sl) 2012-09-20 2024-10-30 The Chinese University Of Hong Kong Neinvazivno določanje metiloma tumorja iz plazme
US10767222B2 (en) * 2013-12-11 2020-09-08 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
WO2016077313A1 (en) * 2014-11-11 2016-05-19 Bgi Shenzhen Multi-pass sequencing
AU2016406443A1 (en) 2016-05-10 2018-11-15 Nec Corporation Demand and supply adjustment system, control apparatus, control method, and program

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006095169A1 (en) * 2005-03-08 2006-09-14 Lingvitae As Method for preparing polynucleotides for analysis
US20120045771A1 (en) * 2008-12-11 2012-02-23 Febit Holding Gmbh Method for analysis of nucleic acid populations
WO2012060595A2 (en) * 2010-11-01 2012-05-10 Lg Electronics Inc. Structure with nanopore and apparatus for determining sequences of nucleic acids including the same
US20130040827A1 (en) * 2011-08-14 2013-02-14 Stephen C. Macevicz Method and compositions for detecting and sequencing nucleic acids

Also Published As

Publication number Publication date
JP7506408B2 (ja) 2024-06-26
IL285795A (en) 2021-09-30
CN107849607A (zh) 2018-03-27
TW202130815A (zh) 2021-08-16
AU2016305103A1 (en) 2018-03-22
MY188348A (en) 2021-12-02
US11319586B2 (en) 2022-05-03
TWI793586B (zh) 2023-02-21
IL257074B (en) 2021-09-30
AU2020202153A1 (en) 2020-04-16
JP2021118691A (ja) 2021-08-12
EP3334843A1 (en) 2018-06-20
TW201718874A (zh) 2017-06-01
AU2016305103B2 (en) 2020-01-02
JP2024113130A (ja) 2024-08-21
AU2023202572B2 (en) 2025-04-10
JP7810455B2 (ja) 2026-02-03
IL294192B1 (en) 2023-04-01
US20220205038A1 (en) 2022-06-30
IL294192B2 (en) 2023-08-01
WO2017025059A1 (en) 2017-02-16
SA518390907B1 (ar) 2021-09-08
EP3334843A4 (en) 2019-01-02
HK1245850A1 (zh) 2018-08-31
AU2023202572A1 (en) 2023-05-18
IL294192A (en) 2022-08-01
AU2020202153B2 (en) 2023-02-02
JP2018531583A (ja) 2018-11-01
AU2016305103C1 (en) 2020-06-04
IL285795B (en) 2022-07-01
US20170044606A1 (en) 2017-02-16
JP6867045B2 (ja) 2021-04-28
CA2995422A1 (en) 2017-02-16
CN115433769A (zh) 2022-12-06
CN107849607B (zh) 2022-10-11
IL257074A (en) 2018-03-29
HK1248286A1 (zh) 2018-10-12

Similar Documents

Publication Publication Date Title
JP7810455B2 (ja) 血漿dnaの単分子配列決定
TWI867325B (zh) 利用血漿中dna的尺寸及數量畸變以偵測癌症
JP6161607B2 (ja) サンプルにおける異なる異数性の有無を決定する方法
TWI727938B (zh) 血漿粒線體dna分析之應用
CN108026572A (zh) 游离dna的片段化模式的分析
CN107630081A (zh) 母体血浆中胎儿dna分数的基于大小的分析
CN110023509A (zh) 基因型分型测定中的非独特条形码
HK40083172A (en) Single-molecule sequencing of plasma dna
HK1245850B (en) Single-molecule sequencing of plasma dna
HK1246361B (zh) 母体血浆中胎儿dna分数的基於大小的分析
HK1245842B (zh) 母体血浆中胎儿dna分数的基於大小的分析
HK1237825A1 (en) Applications of plasma mitochondrial dna analysis
HK1240279B (zh) 使用血浆dna的尺寸和数目畸变检测癌症
HK1205204B (zh) 快速非整倍性检测