CN107849097A - 杀虫基因和使用方法 - Google Patents

杀虫基因和使用方法 Download PDF

Info

Publication number
CN107849097A
CN107849097A CN201680043643.1A CN201680043643A CN107849097A CN 107849097 A CN107849097 A CN 107849097A CN 201680043643 A CN201680043643 A CN 201680043643A CN 107849097 A CN107849097 A CN 107849097A
Authority
CN
China
Prior art keywords
ser
thr
leu
gly
asn
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201680043643.1A
Other languages
English (en)
Inventor
J·帕克斯
K·B·罗伯茨
R·E·赛耶
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agricultural Bio Community Ltd By Share Ltd
AgBiome Inc
Original Assignee
Agricultural Bio Community Ltd By Share Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agricultural Bio Community Ltd By Share Ltd filed Critical Agricultural Bio Community Ltd By Share Ltd
Publication of CN107849097A publication Critical patent/CN107849097A/zh
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

提供了具有杀虫活性的组合物及其使用方法。组合物包括具有杀虫活性的分离的和重组的多肽，编码多肽的重组和合成的核酸分子，包含核酸分子的DNA构建体和载体，包含载体的宿主细胞和针对多肽的抗体。编码多肽的核苷酸序列可以用于DNA构建体或表达盒中，用于在目的生物中的转化和表达。提供的组合物和方法可用于生产具有增强的害虫抗性或耐受性的生物。还提供了包含编码本发明的杀虫蛋白质的核苷酸序列的转基因植物和种子。这种植物对昆虫和其他害虫具有抗性。提供了用于产生本文公开的各种多肽以及使用这些多肽控制或杀死害虫的方法。还包括用于检测样品中的本发明多肽的方法和试剂盒。

Description

杀虫基因和使用方法
技术领域
本发明涉及用于防治害虫，特别是植物害虫的方法和组合物。
相关申请的交叉引用
本申请要求于2015年6月22日提交的美国临时申请序列号62/182,855的权益，其通过引用整体并入本文。
经由EFS-WEB作为文本文件提交的序列表的提及
序列表的官方拷贝作为ASCII格式化序列表经由EFS-Web以电子方式提交，文件名为AGB015PCT-SeqListing-1010353.txt，于2016年6月16日创建，具有952,518字节的大小，且与说明书同时提交。包含在该ASCII格式化文件中的序列表是说明书的一部分，并且通过引用整体并入本文。
背景
害虫、植物疾病和杂草对作物可以是严重的威胁。由于害虫和疾病的损失据估计占全世界农业生产的37％，其中由于昆虫、细菌和其他生物的占13％。
毒素是在微生物致病性和/或宿主免疫应答逃避中起重要作用的毒力决定簇。来自革兰氏阳性细菌芽孢杆菌属(Bacillus)，特别是苏云金芽孢杆菌(Bacillusthuringensis)的毒素已被用作杀昆虫蛋白质。目前的策略使用表达这些毒素的基因来产生转基因作物。表达杀昆虫蛋白质毒素的转基因作物被用于对抗来自昆虫的作物损害。
尽管使用芽孢杆菌毒素已成功地控制昆虫，但在其中这些毒素已大量使用的世界上许多地方的一些靶害虫中已发展了针对Bt毒素的抗性。解决这个问题的一个方法是播种Bt作物与常规非Bt作物(避难所)的交替行。避免或减缓昆虫抗性发展的可替代方法是在转基因植物中堆积具有针对昆虫的不同作用方式的杀昆虫基因。使用表达杀昆虫蛋白质毒素的转基因作物的目前策略越来越重视在已经衍生自细菌苏云金芽孢杆菌的那些毒素之外发现新毒素。这些毒素可以证明可用作衍生自苏云金芽孢杆菌的那些毒素的替代物用于在昆虫和害虫抗性转基因植物中的应用。因此，需要新的毒素蛋白质。
概述
提供了具有杀虫活性的组合物及其使用方法。组合物包括具有杀虫活性的分离的和重组的多肽序列，编码杀虫多肽的重组和合成的核酸分子，包含核酸分子的DNA构建体，包含核酸分子的载体，包含载体的宿主细胞和针对杀虫多肽的抗体。编码本文提供的多肽的核苷酸序列可以用于DNA构建体或表达盒中，用于在目的生物包括微生物和植物中的转化和表达。
本文提供的组合物和方法可用于生产具有增强的害虫抗性或耐受性的生物。这些生物和包含生物的组合物对于农业目的是期望的。还提供了包含编码本发明的杀虫蛋白质的核苷酸序列的转基因植物和种子。这种植物对昆虫和其他害虫具有抗性。
提供了用于产生本文公开的各种多肽以及使用这些多肽控制或杀死害虫的方法。还包括用于检测样品中的本发明多肽的方法和试剂盒。
发明详述
现在将在下文中更全面地描述本发明。实际上，这些发明可以以许多不同的形式来体现，并且不应被解释为限制于本文阐述的实施方案；相反，提供这些实施方案使得本公开内容将满足适用的法律要求。
受益于上述说明中呈现的教导，这些发明所属领域的技术人员将会想到本文阐述的本发明的许多修改和其他实施方案。因此，应理解本发明并不限于所公开的特定实施方案，并且修改和其他实施方案预期被包括在所附权利要求的范围内。虽然本文采用了特定的术语，但它们仅以一般的和描述性的含义使用，而不是用于限制的目的。
I.多核苷酸和多肽
提供了用于对生物体赋予杀虫活性的组合物和方法。经修饰的生物体显示出杀虫抗性或耐受性。提供了保留杀虫活性的重组杀虫蛋白质或多肽及其片段和变体，并且包括在SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223和/或224中阐述的那些。杀虫蛋白质针对害虫包括昆虫、真菌、线虫等等是生物学活性的(例如杀虫的)。编码杀虫多肽、包括例如SEQID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223和/或224或其活性片段或变体的核苷酸，可以用于产生转基因生物，例如植物和微生物。杀虫蛋白质针对害虫包括昆虫、真菌、线虫等等是生物学活性的(例如杀虫的)。编码杀虫多肽、包括例如SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223和/或224，或其活性片段或变体的多核苷酸可以用于产生转基因生物，例如植物和微生物。经转化的生物的特征在于包含至少一个稳定掺入的DNA构建体的基因组，所述DNA构建体包含本文公开的杀虫蛋白质的编码序列。在一些实施方案中，编码序列可操作地连接至驱动所编码的杀虫多肽的表达的启动子。相应地，提供了经转化的微生物、植物细胞、植物组织、植物、种子和植物部分。各种多肽、其活性变体及其片段和编码其的多核苷酸在下表1中阐述。如表1所示，提供了各种形式的多肽。提供了全长杀虫多肽以及原始全长序列的修饰形式(即变体)。表1进一步指示“CryBP1”序列。这样的序列(SEQ ID NO:11和93)包含可以与一些毒素基因相关的辅助多肽。在这种情况下，CryBP1序列可以单独使用或与本文提供的任何杀虫多肽组合使用。表1进一步提供了Split-Cry C末端多肽(SEQ ID NO:106)。此类序列包含与Cry类毒素基因的C末端具有同源性的下游蛋白质的序列，并且通常在并非全长且缺失预期的C末端区域的Cry基因之后发现。
i.杀虫蛋白质的类别
本文提供的杀虫蛋白质和编码其的核苷酸序列在用于影响害虫的方法中是有用的。即，本发明的组合物和方法在农业中用于控制或杀死害虫，包括许多作物植物的害虫。本文提供的杀虫蛋白质是来自细菌的毒素蛋白质，并显示出针对某些害虫的活性。杀虫蛋白质来自几个毒素类别，包括Cry、Cyt、BIN、Mtx毒素。关于本文提供的各种SEQ ID NOS的特定蛋白质分类参见例如表1。另外，在本公开内容自始至终参考Pfam数据库条目。Pfam数据库是蛋白质家族的数据库，每个蛋白质家族都由多重序列比对和概况隐马尔可夫模型表示。Finn等人(2014)Nucl.Acid Res.Database Issue 42:D222-D230。
苏云金芽孢杆菌(Bt)是革兰氏阳性细菌，在其生长的孢子形成期过程中以晶体包涵体形式产生杀昆虫蛋白质。苏云金芽孢杆菌(Bt)的蛋白质包涵体被称为晶体蛋白质或δ-内毒素(或Cry蛋白)，其对昆虫纲和其他无脊椎动物的成员是有毒的。类似地，Cyt蛋白是来自Bt的伴孢包涵体蛋白，其显示出溶血(细胞溶解)活性或与已知的Cyt蛋白具有明显的序列相似性。这些毒素对其靶生物是高度特异性的，对人、脊椎动物和植物无害。
Cry毒素的结构显示有五个保守的氨基酸块，主要集中在结构域的中心或结构域之间的连接处。Cry毒素由三个结构域组成，每个结构域具有特定的功能。结构域I是七个α-螺旋的束，其中中央螺旋被六个外部螺旋完全包围。该结构域牵涉膜中的通道形成。结构域II显示为三个反向平行的β-折叠片的三角形柱，其与免疫球蛋白的抗原结合区相似。结构域III包含β-夹心形式的反向平行β-链。毒素蛋白质的N端部分负责其毒性和特异性，并且含有5个保守区域。C末端部分通常是高度保守的，并且可能负责晶体形成。参见例如美国专利号8,878,007。
苏云金芽孢杆菌菌株显示针对不同昆虫目(鳞翅目(Lepidoptera)、双翅目(Diptera)、鞘翅目(Coleoptera)、膜翅目(Hymenoptera)、同翅目(Homoptera)、虱目(Phthiraptera)例如食毛目(Mallophaga)和蜱螨亚纲(Acari))和其他无脊椎动物(线虫动物(Nemathelminthes)、扁形动物(Platyhelminthes)和Sarocomastebrates)的广泛范围的特异性。基于对各种昆虫和无脊椎动物群体的毒性，cry蛋白已被分类成几个组。一般地，CryI表现出对鳞翅目的毒性，CryII表现出对鳞翅目和双翅目的毒性，CryIII表现出对鞘翅目的毒性，Cry IV表现出对双翅目的毒性，并且Cry V和Cry VI表现出对线虫的毒性。基于氨基酸同一性，新的Cry蛋白可以得到鉴定并且分配到一个Cry组中。参见例如，通过引用并入本文的Bravo，A.(1997)J.of Bacteriol.179:2793-2801；Bravo等人(2013)Microb.Biotechnol.6:17-26。
超过750种不同的cry基因序列已被分成73个组(Cry1-Cry73)，而且这个基因家族的新成员还在不断被发现(Crickmore等人(2014)www.btnomenclature.info/)。cry基因家族由可以具有不同作用模式的几个系统发育上无关的蛋白质家族组成：三结构域Cry毒素家族，杀蚊Cry毒素家族，二元样毒素家族和Cyt毒素家族(Bravo等人，2005)。一些Bt菌株产生另外的杀昆虫毒素，VIP毒素。还参见Cohen等人(2011)J.Mol.Biol.413:4-814；在万维网上lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/处找到的Crickmore等人(2014)苏云金芽孢杆菌毒素命名法；Crickmore等人(1988)Microbiol.Mol.Biol.Rev.62:807-813；Gill等人(1992)Ann.Rev.Entomol.37:807-636；Goldbert等人(1997)Appl.Environ.Microbiol.63:2716-2712；Knowles等人(1992)Proc.R.Soc.Ser.B.248:1-7；Koni等人(1994)Microbiology 140:1869-1880；Lailak等人(2013)Biochem.Biophys.Res.Commun.435:216-221；Lopez-Diaz等人(2013)Environ.Microbiol.15:3030-3039；Perez等人(2007)Cell.Microbiol.9:2931-2937；Promdonkoy等人(2003)Biochem.J.374:255-259；Rigden(2009)FEBS Lett.583:1555-1560；Schnepf等人(1998)Microbiol.Mol.Biol.Rev.62:775-806；Soberon等人(2013)Peptides 41:87-93；Thiery等人(1998)J.Am.Mosq.Control Assoc.14:472-476；Thomas等人(1983)FEBS Lett.154:362-368；Wirth等人(1997)Proc.Natl.Acad.Sci.U.S.A.94:10536-10540；Wirth等人(2005)Appl.Environ.Microbiol.71:185-189；和Zhang等人(2006)Biosci.Biotechnol.Biochem.70:2199-2204；其各自通过引用整体并入本文。
Cyt指示具有细胞溶解活性的来自苏云金芽孢杆菌的伴孢晶体包涵体蛋白，或与已知Cyt蛋白具有序列相似性的蛋白质。(Crickmore等人(1998)Microbiol.Mol.Biol.Rev.62:807-813.)。该基因被命名为cyt。这些蛋白质与Cry蛋白在结构和活性方面不同(Gill等人(1992)Annu.Rev.Entomol.37:615-636)。Cyt毒素首先在苏云金芽孢杆菌以色列亚种中发现(Goldberg等人(1977)Mosq.News.37:355-358)。在目前的命名法中存在3个Cyt毒素家族，包括11个正模式标本(holotype)毒素(在万维网上lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/处找到的Crickmore等人(2014)苏云金芽孢杆菌毒素命名法)。具有cyt基因的大多数苏云金芽孢杆菌分离物显示针对双翅目昆虫(特别是蚊子和黑蝇)的活性，但也存在靶向鳞翅目或鞘翅目昆虫的苏云金芽孢杆菌菌株中已描述的cyt基因(Guerchicoff等人(1997)Appl.Environ.Microbiol.63:2716-2721)。
通过X射线晶体学解析的Cyt2A的结构显示单一结构域，其中有两个α-螺旋外层缠绕在混合β-折叠周围。Cyt毒素的进一步可用晶体结构支持具有两个α-螺旋发夹的保守α-β结构模型，所述α螺旋发夹侧接含有七至八条β-链的β-折叠核心。(Cohen等人(2011)J.Mol.Biol.413:80 4-814.)。诱变研究将β-折叠残基鉴定为对于毒性是关键的，而螺旋结构域中的突变不影响毒性(Adang等人，Diversity of Bacillus thuringiensis CrystalToxins and Mechanism of Action.In:T.S.Dhadialla和S.S.Gill编辑，Advances inInsect Physiology，Vol.47，Oxford:Academic Press，2014，第39-87页.)。Cyt毒素的代表性结构域是β-内毒素Bac_thur_toxin(Pfam PF01338)。
对于Cyt毒素的作用模式存在多种建议模式，而且这仍然是活跃研究领域。一些Cyt蛋白(Cyt1A)已显示需要辅助蛋白的存在用于结晶。Cyt1A和Cyt2A原毒素在N和C末端的相同位点处被消化蛋白酶加工为稳定的毒素核心。Cyt毒素然后与非饱和的膜脂质(例如磷脂酰胆碱、磷脂酰乙醇胺和鞘磷脂)相互作用。对于Cyt毒素，孔形成和去污剂样膜破裂已被提议作为非排他性的机制；并且一般公认两者均可以取决于毒素浓度而发生，其中较低的浓度有利于寡聚孔，较高的浓度导致膜破裂。(Butko(2003)Appl.Environ.Microbiol.69:2415-2422)在孔形成模型中，Cyt毒素与细胞膜结合，诱导膜囊泡中阳离子选择性通道的形成，导致细胞的胶体-渗透性裂解。(Knowles等人(1989)FEBS Lett.244:259-262；Knowles等人(1992)Proc.R.Soc.Ser.B.248:1-7和Promdonkoy等人(2003)Biochem.J.374:255-259.)在去污剂模型中，在脂双层的表面上存在毒素的非特异性聚集，导致膜分解和细胞死亡。(Butko(2003)同上；Manceva等人(2005)Biochem.44:589-597.)
多个研究已显示Cyt毒素与其他苏云金芽孢杆菌毒素，特别是Cry、Bin和Mtx毒素之间的协同活性。这种协同作用甚至已显示克服昆虫对其他毒素的抗性。(Wirth 1997，Wirth 2005，Thiery 1998，Zhang 2006.)。Cry毒素的Cyt协同效应推测涉及Cyt1A与Cry毒素结构域II在溶液中或膜平面上的结合，促进Cry毒素预孔寡聚物以形成。该寡聚物的形成不依赖于Cyt寡聚化、结合或插入。(Lailak 2013，Perez 2007，Lopez-Diaz 2013.)
在营养生长期间，与Cry蛋白无关的许多杀虫蛋白质由苏云金芽孢杆菌和蜡状芽孢杆菌(B.cereus)的一些菌株产生(Estruch等人(1996)Proc Natl Acad Sci USA 93:5389–5394；Warren等人(1994)WO 94/21795)。这些营养型杀昆虫蛋白质或Vip不形成伴孢晶体蛋白质，并且明显由细胞分泌。Vip目前被排除在Cry蛋白命名法之外，因为它们不是晶体形成蛋白。术语VIP在含义上是一个误称，因为一些苏云金芽孢杆菌Cry蛋白在营养生长期间以及在静止期和孢子形成期过程中也产生，最著名的是Cry3Aa。苏云金芽孢杆菌基因组中的Vip基因的位置据报道位于也编码cry基因的大质粒中(Mesrati等人(2005)FEMSMicrobiol.Lett.244(2):353-358)。可在万维网上的lifesci.sussex.ac.uk处以路径“/home/Neil_Crickmore/Bt/”和在：“btnomenclature.info/”处找到Bt毒素命名法的网站。还参见Schnepf等人(1998)Microbiol.Mol.Biol.Rev.62(3):775-806。这些参考文献通过引用并入本文。
迄今为止已鉴定了四类Vip。一些Vip基因形成二元双组分蛋白复合物；“A”组分通常是“活动”部分，“B”组分通常是“结合”部分。(Pfam pfam.xfam.org/family/PF03495)。Vip1和Vip4蛋白一般含有二元毒素B蛋白结构域。Vip2蛋白一般含有二元毒素A蛋白结构域。
Vip1和Vip2蛋白是显示出针对鞘翅目的毒性的二元毒素的两个组分。Vip1Aa1和Vip2Aa1针对玉米根虫，特别是玉米根叶甲是有非常高活性的(Diabrotica virgifera)和长角叶甲(Diabrotica longicornis)(Han等人(1999)Nat.Struct.Biol.6:932-936；Warren GW(1997)“Vegetative insecticidal proteins:novel proteins for controlof corn pests”In:Carozzi NB，Koziel M(编辑)Advances in insect control，the roleof transgenic plants；Taylor&Francis Ltd，London，第109-121页)。膜结合的95kDaVip1多聚体提供了用于52kDa vip2ADP-核糖酶进入靶西方玉米根虫细胞的细胞质的途径(Warren(1997)同上)。NAD依赖性ADP-核糖基转移酶Vip2可能在Arg177处修饰单体肌动蛋白以阻断聚合，导致肌动蛋白细胞骨架的损失，并且由于体内肌动蛋白丝内的快速亚单位交换导致最终细胞死亡(Carlier M.F.(1990)Adv.Biophys.26:51-73)。
如同Cry毒素，活化的Vip3A毒素是能够在膜中制备稳定离子通道的成孔蛋白质(Lee等人(2003)Appl.Environ.Microbiol.69:4648–4657)。Vip3蛋白针对几种主要的鳞翅目害虫具有活性(Rang等人(2005)Appl.Environ.Microbiol.71(10):6276-6281；Bhalla等人(2005)FEMS Microbiol.Lett.243:467-472；Estruch等人(1998)WO 9844137；Estruch等人(1996)Proc Natl Acad Sci USA 93:5389-5394；Selvapandiyan等人(2001)Appl.Environ Microbiol.67:5855–5858；Yu等人(1997)Appl.Environ Microbiol.63:532–536)。Vip3A针对黑切根虫(Agrotis ipsilon)、草地贪夜蛾(Spodopterafrugiperda)、甜菜夜蛾(Spodoptera exigua)、烟芽夜蛾(Heliothis virescens)和玉米穗虫(Helicoverpa zea)有活性(Warren等人(1996)WO 96/10083；Es truch等人(1996)ProcNatl Acad Sci USA 93:5389–5394)。如同Cry毒素，Vip3A蛋白质必须在特异性膜蛋白质的中肠上皮表面上识别之前被蛋白酶激活，所述特异性膜蛋白质不同于由Cry毒素识别的那些。
毒素蛋白质的MTX家族的特征在于保守结构域ETX_MTX2(pfam 03318)的存在。该家族的成员与来自球形芽孢杆菌(Bacillus sphaericus)的杀蚊毒素Mtx2和Mtx3以及来自产气荚膜梭菌(Clostridium perfringens)的ε毒素ETX共享序列同源性(Cole等人(2004)Nat.Struct.Mol.Biol.11:797-8；Thanabalu等人(1996)Gene 170:85-89)。MTX样蛋白在结构上不同于三结构域Cry毒素，因为它们具有延长且主要是基于β-折叠的结构。然而，类似于三结构域毒素，MTX样蛋白被认为在靶细胞的膜上形成孔(Adang等人(2014)，同上)。与三结构域Cry蛋白不同，MTX样蛋白的长度小得多，范围从267个氨基酸(Cry23)到340个氨基酸(Cry15A)。
迄今为止，仅15种属于MTX样毒素家族的蛋白质被分配了Cry名称，因而其与三结构域Cry家族相比是相对较小的类别(Crickmore等人(2014)同上；Adang等人(2014)同上)。MTX样毒素家族的成员包括Cry15、Cry23、Cry33、Cry38、Cry45、Cry46、Cry51、Cry60A、Cry60B和Cry64。这个家族显示出一系列的杀昆虫活性，包括针对鳞翅目和鞘翅目的昆虫害虫的活性。这个家族的一些成员可以与其他蛋白质形成二元合作关系，这对于杀昆虫活性可能是需要的或也可能是不需要的。
Cry15是一种在苏云金芽孢杆菌血清变型thompsoni HD542中鉴定的34kDA蛋白质；它与大约40kDa的一种无关蛋白质一起天然存在于晶体中。编码Cry15及其伙伴蛋白的基因在操纵子中一起排列。Cry15单独已显示具有针对鳞翅目昆虫害虫的活性，所述昆虫害虫包括烟草天蛾(Manduca sexta)、苹果蠹蛾(Cydia pomonella)和菜粉蝶(Pierisrapae)，其中所述40kDA蛋白的存在已显示仅增加Cry15针对苹果蠹蛾的活性(Brown K.和Whiteley H.(1992)J.Bacteriol.174:549-557；Naimov等人(2008)Appl.Environ.Microbiol.74:7145–7151)。需要进一步的研究来阐明Cry15的伙伴蛋白质的功能。类似地，Cry23是一种29kDa蛋白质，其已显示与其伙伴蛋白质Cry37一起具有针对鞘翅目害虫赤拟谷盗(Tribolium castaneum)和日本弧丽金龟(Popillia japonica)的活性(Donovan等人(2000)美国专利号6,063,756)。
MTX样家族的新成员仍在不断被鉴定出来。最近在苏云金芽孢杆菌血清变型tolworthi菌株Na205-3的基因组中鉴定了ETX_MTX毒素基因。发现该菌株针对鳞翅目害虫棉铃虫(Helicoverpa armigera)是有毒性的，并且它还含有Cry1、Cry11、Vip1、Vip2和Vip3的同系物(Palma等人(2014)Genome Announc.2(2):e00187-14.2014年3月13日在线发表于doi:10.1128/genomeA.00187-14；PMCID:PMC3953196处)。因为MTX样蛋白相对于三结构域Cry蛋白具有独特的结构域结构，所以它们被认为具有独特的作用模式，从而使其成为昆虫防治和抵抗昆虫抗性中有价值的工具。
细菌细胞产生针对宿主和非宿主生物具有不同特异性的大量毒素。已在许多细菌家族中鉴定了二元毒素的大家族，包括具有针对昆虫害虫的活性的毒素。(Poopathi和Abidha(2010)J.Physiol.Path.1(3):22-38)。球形赖氨酸芽孢杆菌(Lysinibacillussphaericus)(Ls)，以前称为球形芽孢杆菌(Ahmed等人(2007)Int.J.Syst.Evol.Microbiol.57:1117-1125)，是众所周知的昆虫生物防治菌株。Ls产生几种杀昆虫蛋白质，包括高度有效的二元复合物BinA/BinB。这种二元复合物在Ls细胞中形成伴孢晶体，对于双翅目昆虫、特别是蚊子具有强而特异的活性。在一些地区，已报道了对现有Ls杀蚊菌株的昆虫抗性。具有不同靶特异性或克服昆虫抗性的能力的新二元毒素的发现具有重大意义。
Ls二元杀昆虫蛋白质复合物含有两种主要的多肽，即分别命名为BinA和BinB的42kDa多肽和51kDa多肽(Ahmed等人(2007)同上)。这两种多肽协同作用以对其靶赋予毒性。作用方式涉及所述蛋白质与幼虫中肠内受体的结合。在一些情况下，所述蛋白质通过在幼虫肠道中的蛋白酶消化来修饰，产生活化形式。BinB组分被认为涉及结合，而BinA组分赋予毒性(Nielsen-LeRoux等人(2001)Appl.Environ.Microbiol.67(11):5049–5054)。当分别克隆和表达时，BinA组分对蚊子幼虫是有毒性的，而BinB组分则没有。然而，所述蛋白质的共同施用显著增加毒性(Nielsen-LeRoux等人(2001)同上)。
已从细菌来源中描述了少量Bin蛋白同系物。Priest等人(1997)Appl.Environ.Microbiol.63(4):1195-1198描述了鉴定新的Ls菌株的杂交努力，虽然他们鉴定的大部分基因编码的蛋白质与已知的BinA/BinB蛋白质相同。BinA蛋白含有被称为毒素10超家族结构域的确定的保守结构域。该毒素结构域最初通过其在BinA和BinB中的存在得到确定。这两种蛋白质均具有该结构域，尽管BinA和BinB之间的序列相似性在这个区域是有限的(<40％)。也具有杀昆虫活性的Cry49Aa蛋白也具有该结构域(下文描述)。
Ls的Cry48Aa/Cry49Aa二元毒素具有杀死致倦库蚊(Culex quinquefasciatus)幼虫的能力。这些蛋白质属于这样一个蛋白质结构类别，该类别与苏云金芽孢杆菌(Bt)的Cry蛋白复合物(一个众所周知的杀昆虫蛋白质家族)具有一些相似性。Bt的Cry34/Cry35二元毒素也已知会杀死昆虫，包括西方玉米根虫(一种重要的玉米害虫)。已鉴定了几种变体的Cry34是一种小型(14kDa)多肽，而Cry35(也由几种变体编码)是一种44kDa的多肽。这些蛋白质与BinA/BinB蛋白质组具有一些序列同源性，并且被视为在进化上是相关的(Ellis等人(2002)Appl.Environ.Microbiol.68(3):1137-1145)。
磷脂酰肌醇磷脂酶C蛋白质(PI-PLC；也称为磷脂酰肌醇磷脂酶C)是更大的磷脂酶C蛋白质组的成员。这些蛋白质中的许多在信号转导中起重要作用，作为正常细胞生理学的一部分。几种重要的细菌毒素还含有与这些蛋白具有相似性的结构域(Titball，R.W.(1993)Microbiological Reviews.57(2):347-366))。重要的是，这些蛋白质牵涉通过BtCry蛋白在昆虫细胞中毒期间的信号放大(Valaitis，A.P.(2008)Insect Biochemistryand Molecular Biology.38:611-618)。
PI-PLC毒素类别在芽孢杆菌分离株中出现，通常与其他描述的毒素类别(例如二元毒素)的同源物共同出现。这类序列与磷脂酰肌醇磷酸二酯酶(也称为磷脂酰肌醇特异性磷脂酶C-PI-PLC)具有同源性。Heinz等人(Heinz等人(1995)The EMBO Journal.14(16):3855-3863)解决了蜡样芽孢杆菌PI-PLC的晶体结构及其活性位点。通过等人使用定点诱变、动力学和晶体结构分析(等人(1997)Biochemistry.36(42):12802-13)研究了蜡状芽孢杆菌PI-PLC活性位点氨基酸残基在催化和底物结合中的作用。
这些PI-PLC毒素蛋白质含有PLC样磷酸二酯酶、TIMβ/α-桶结构域(IPR017946)和/或磷脂酶C，磷脂酰肌醇特异性X结构域(IPR000909)(也称为PI-PLC X-盒结构域)。我们也已看到具有这些结构域与其他典型芽孢杆菌蛋白质毒素结构域组合的蛋白质。该列表最通常包括凝集素结构域(IPR000772)，可以以一个或多个拷贝存在并且被认为结合细胞膜的糖结合结构域，以及杀昆虫晶体毒素(IPR008872)(也被称为毒素10或P42)，这是二元毒素的限定结构域。这个结构域结构的一个例子在序列APG00732中。
这种PI-PLC类别的毒素之前在美国专利号8,318,900B2 SEQ ID NO 30(DNA)和79(氨基酸)，美国专利公开号20110263488A1 SEQ ID NO 8(DNA)和9(氨基酸)，以及美国专利号8,461,421B2 SEQ ID NO 3(DNA)和63(氨基酸)中有定义。
本文提供了来自这些毒素类别的杀虫蛋白质。杀虫蛋白质根据其结构、与已知毒素的同源性和/或其杀虫特异性进行分类。
ii.杀虫蛋白质和编码其的多核苷酸的变体和片段
本发明的杀虫蛋白质或多肽包括在SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223和/或224中所示的那些及其片段和变体。“杀虫毒素”或“杀虫蛋白质”或“杀虫多肽”是指针对一种或多种害虫(包括昆虫、真菌、线虫等等)有活性的毒素或蛋白质或多肽，使得所述害虫被杀死或受到控制。
“分离的”或“纯化的”多肽或蛋白质或其生物学活性部分基本上或实质上不含如在其天然存在的环境中发现的，通常伴随所述多肽或蛋白质或者与所述多肽或蛋白质相互作用的组分。因此，分离的或纯化的多肽或蛋白质当通过重组技术产生时基本上不含其他细胞材料或培养基，或者当化学合成时基本上不含化学前体或其他化学物质。基本上不含细胞物质的蛋白质包括具有小于约30％、20％、10％、5％或1％(按干重计)的污染蛋白质的蛋白质制剂。当重组产生本发明的蛋白质或其生物学活性部分时，理想地培养基占化学前体或非目的蛋白质的化学物质的少于约30％、20％、10％、5％或1％(按干重计)。
术语“片段”指本发明的多肽序列的一部分。“片段”或“生物学活性部分”包括包含足够数目的邻接氨基酸残基以保留生物学活性(即具有杀虫活性)的多肽。杀虫蛋白质的片段包括比全长序列更短的那些，其或者是由于使用替代的下游起始位点所致，或者是由于产生具有杀虫活性的较短蛋白质的加工所致。加工可以在蛋白质在其中表达的生物体中，或者在摄入蛋白质后在害虫中发生。蛋白质片段的实例可以在表1中找到。杀虫蛋白质的生物学活性部分可以是例如下述任一序列的长度上10、25、50、100、150、200、250个或更多个氨基酸的多肽：SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223和/或224。这些生物学活性部分可以通过重组技术制备并且评估杀虫活性。如本文使用的，片段包含SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223和/或224的至少8个邻接氨基酸。
细菌基因(包括编码本文公开的杀虫蛋白质的那些)在开放读码框的开始附近经常具有多个甲硫氨酸起始密码子。通常，在这些起始密码子中的一个或多个处的翻译起始将导致功能蛋白质的生成。这些起始密码子可以包括ATG密码子。然而，细菌例如芽孢杆菌属菌种(Bacillus sp.)也将密码子GTG识别为起始密码子，并且在GTG密码子处起始翻译的蛋白质在第一个氨基酸处含有甲硫氨酸。在极少数情况下，细菌系统中的翻译可以在TTG密码子处起始，尽管在这种情况下TTG编码甲硫氨酸。此外，通常不能事先确定这些密码子中的哪一个在细菌中天然使用。因此，应理解使用替代甲硫氨酸密码子之一也可以导致杀虫蛋白质的生成。这些杀虫蛋白质涵盖在本发明中，并且可以用于本文公开的方法中。应理解当在植物中表达时，有必要将可替代起始密码子改变为ATG用于正确的翻译。
在各种实施方案中，本文提供的杀虫蛋白质包括由全长核苷酸序列推导的氨基酸序列，以及由于使用替代的下游起始位点而比全长序列更短的氨基酸序列。因此，本发明的核苷酸序列和/或包含本发明的核苷酸序列的载体、宿主细胞和植物(以及制备和使用本发明的核苷酸序列的方法)可以包含编码替代起始位点的核苷酸序列。
可认识到可以对本文提供的杀虫多肽作出修饰，产生变体蛋白质。通过应用定点诱变技术可以引入由人设计的变化。可替代地，也可以鉴定落入本发明范围内的与本文公开的序列在结构和/或功能上相关的天然、然而迄今未知的或迄今未鉴定的多核苷酸和/或多肽。保守氨基酸取代可以在不改变杀虫蛋白质功能的非保守区域中进行。可替代地，可以进行改善毒素活性的修饰。通过结构域III交换修饰Cry毒素在一些情况下已得到了一些杂合毒素，其针对某些昆虫物种具有改善的毒性。因此，结构域III交换可以是改善Cry毒素的毒性或产生针对下述害虫具有毒性的新型杂合毒素的有效策略，所述害虫对于亲本Cry毒素未显示敏感性。结构域II环序列的定点诱变可以导致具有增加的杀昆虫活性的新毒素。结构域II环区是初始Cry毒素的关键结合区，其是具有改善的杀昆虫性质的Cry毒素的诱变和选择的合适靶。Cry毒素的结构域I可以进行修饰，以引入蛋白酶切割位点而改善针对某些害虫的活性。用于在大量cry基因中改组这三个不同结构域的策略和高通量生物测定筛选方法可以提供具有改善的或新型的毒性的新型Cry毒素。
如所指出的，本文公开的多肽的片段和变体将保留杀虫活性。杀虫活性包括组合物实现减少靶害虫的发生或活性的可观察效应的能力，包括例如导致至少一个害虫的死亡，或者害虫生长、进食或正常生理发育的明显下降。数目、害虫生长、进食或正常发育的这些下降可以包含任何统计学上显著的降低，包括例如约5％、10％、15％、20％、25％、30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、85％、90％、95％或更多的降低。认识到杀虫活性相对于天然蛋白质的活性而言可以不同或改善，或者它可以保持不变，只要保留杀虫活性即可。用于测量杀虫活性的方法是本领域众所周知的。参见例如Czapla和Lang(1990)J.Econ.Entomol.83:2480-2485；Andrews等人(1988)Biochem.J.252:199-206；Marrone等人(1985)J.of Economic Entomology 78:290-293；和美国专利号5,743,477，所有这些文献通过引用整体并入本文。
本公开内容的多肽变体包括具有与下述中任一个氨基酸序列有至少约60％、约65％、约70％、约75％、约80％、约85％、约86％、约87％、约88％、约89％、约90％、约91％、约92％、约93％、约94％、约95％、约96％、约97％、约98％或约99％相同、并且保留杀虫活性的氨基酸序列的多肽：SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223和/或224。注意，表1提供了SEQ ID NO:1-224中每一个的变体多肽(和编码其的多核苷酸)的非限制性实例。本发明的杀虫多肽的生物学活性变体可以有少至约1-15个氨基酸残基，少至约1-10，例如约6-10、少至5、少至4、少至3个、少至2个、或少至1个氨基酸残基的差异。在具体的实施方案中，多肽可以包含N'末端或C'末端截短，其可以包括从多肽的N'或C'末端缺失至少10、15、20、25、30、35、40、45、50个氨基酸或更多个氨基酸。
表2提供了基于PFAM数据的在SEQ ID NO:1-224中发现的蛋白质结构域。结构域描述和在给定SEQ ID NO内的位置两者都在表2中提供。在特定的实施方案中，包含SEQ IDNO:1-224中任何一个的活性变体可以与SEQ ID NO:1-224中任何一个有至少70％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％序列同一性，并且进一步包含表2中所示的保守结构域中的至少一个。例如，在一个实施方案中，活性变体将与SEQ ID NO:3有至少70％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％的序列同一性，并且进一步包含在位置207-400处的天然氨基酸。
表2.SEQ ID NO:1-224每一个中的PFAM结构域的概述
还提供了编码本文公开的杀虫多肽的重组或合成核酸。特别有兴趣的是已被设计用于在目的植物中表达的核酸序列。即，可以对核酸序列进行优化以增加宿主植物中的表达。本发明的杀虫蛋白质可以被反向翻译以产生包含为了在特定宿主例如农作物中表达而优化的密码子的核酸。在另一个实施方案中，可以将编码本文提供的多肽的多核苷酸进行优化，用于增加在经转化的植物中的表达。即，多核苷酸可以使用植物偏好的密码子来合成用于改善表达。关于宿主偏好的密码子使用的讨论，参见例如Campbell和Gowri(1990)Plant Physiol.92:1-11。本领域可获得用于合成植物偏好基因的方法。参见例如通过引用并入本文的美国专利号5,380,831和5,436,391，以及Murray等人(1989)Nucleic AcidsRes.17:477-498。通过经转化的植物(例如双子叶植物或单子叶植物)表达这种编码序列将导致产生杀虫多肽，并且在植物中赋予针对害虫的增加抗性。编码本发明杀虫蛋白质的重组和合成核酸分子不包括编码该蛋白的天然存在的细菌序列。
“重组多核苷酸”或“重组核酸”包含两种或更多种化学连接的核酸区段的组合，其在自然界中未发现直接连接。“直接连接”是指两个核酸区段直接相邻并且通过化学连键彼此连接。在具体的实施方案中，重组多核苷酸包含目的多核苷酸或其变体或片段，使得另外的化学连接核酸区段位于目的多核苷酸的5'、3'或内部。可替代地，重组多核苷酸的化学连接的核酸区段可以通过序列缺失而形成。另外的化学连接的核酸区段或缺失以连接所连接的核酸区段的序列可以具有任何长度，包括例如1、2、3、4、5、6、7、8、9、10、15、20个或更多个核苷酸。用于制备这种重组多核苷酸的各种方法包括化学合成或通过基因工程技术操作分离的多核苷酸区段。在具体的实施方案中，重组多核苷酸可以包含重组DNA序列或重组RNA序列。“重组多核苷酸或核酸的片段”包含两个或更多个化学连接的氨基酸区段的组合中的至少一种，所述氨基酸区段在自然界中未发现直接连接。
多核苷酸(RNA或DNA)的片段可以编码保留活性的蛋白质片段。在具体的实施方案中，重组多核苷酸或重组多核苷酸构建体的片段包含所述两个或更多个化学连接的或可操作地连接的核酸区段的至少一个连接，所述核酸区段在自然界中未发现直接连接。编码保留杀虫活性的多肽的生物学活性部分的多核苷酸的片段将编码如下所述序列的全长多肽中存在的至少25、30、40、50、60、70、75、80、90、100、110、120、125、130、140、150、160、170、175、180个邻接氨基酸或高达全部数目的氨基酸：SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223和/或224。在具体的实施方案中，此类多肽片段是活性片段，在另外的其他实施方案中，多肽片段包含重组多肽片段。如本文使用的，重组多肽的片段包含两个或更多个化学连接的氨基酸区段的组合中的至少一种，所述氨基酸区段在自然界中未发现直接连接。
“变体”意指基本上相似的序列。对于多核苷酸，变体包含在天然多核苷酸内的一个或多个内部位点处一个或多个核苷酸的缺失和/或添加和/或在天然多核苷酸的一个或多个位点处一个或多个核苷酸的取代。如本文使用的，“天然”多核苷酸或多肽分别包含天然存在的核苷酸序列或氨基酸序列。
也可以通过比较由变体多核苷酸编码的多肽与由参考多核苷酸编码的多肽之间的序列同一性百分比来评估本发明的特定多核苷酸(即参考多核苷酸)的变体。因此，例如，公开了编码与具有下述序列的多肽有给定百分比序列同一性的多肽的分离的多核苷酸SEQID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223或224。任何两个多肽之间的序列同一性百分比可以使用本文其他地方所述的序列比对程序和参数来计算。当通过比较由它们编码的两种多肽共享的百分比序列同一性来评估本发明的任何给定多核苷酸对时，两种编码多肽之间的序列同一性百分比为与下述序列有至少约40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％，或者更高序列同一性：SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223或224。
在其他实施方案中，本文提供的多核苷酸的变体与天然序列的差别有至少1、2、3、4、5、6、7、8、9、10个或更多个核苷酸。
变体多核苷酸和蛋白质还涵盖衍生自诱变和重组程序如DNA改组的序列和蛋白质。使用这样的程序，操纵本文公开的一种或多种不同的杀虫蛋白质(SEQ ID NO:1-224)，以产生具有所需性质的新杀虫蛋白质。以这种方式，由相关序列多核苷酸群体生成重组多核苷酸文库，所述相关序列多核苷酸包含具有基本序列同一性并且可在体外或体内同源重组的序列区域。例如，使用这种方法，编码目的结构域的序列基序可以在本文提供的杀虫序列和其他已知的杀虫基因之间改组，以获得编码具有改善的目的性质的蛋白质的新基因，例如在酶的情况下有增加的Km。这种DNA改组的策略是本领域已知的。参见例如，Stemmer(1994)Proc.Natl.Acad.Sci.USA 91:10747-10751；Stemmer(1994)Nature 370:389-391；Crameri等人(1997)Nature Biotech.15:436-438；Moore等人(1997)J.Mol.Biol.272:336-347；Zhang等人(1997)Proc.Natl.Acad.Sci.USA 94:4504-4509；Crameri等人(1998)Nature 391:288-291；以及美国专利号5,605,793和5,837,458。“改组”核酸是通过改组程序例如本文所述的任何改组程序产生的核酸。通过例如以人工和任选递归的方式重组(物理上或实质上)两个或更多个核酸(或字符串)来产生改组的核酸。一般地，在改组过程中使用一个或多个筛选步骤来鉴定目的核酸；这个筛选步骤可以在任何重组步骤之前或之后执行。在一些(但不是全部)改组实施方案中，理想的是在选择之前执行多轮重组，以增加待筛选的库的多样性。重组和选择的整个过程任选递归地重复。根据上下文，改组可以指重组和选择的整个过程，或可替代地，可以简单地指整个过程的重组部分。
在一个实施方案中，提供了获得编码包含杀虫活性的改善的多肽的多核苷酸的方法，其中所述改善的多肽具有超过SEQ ID NO:1-224中任何一个的至少一种改善的性质。此类方法可包括(a)重组多个亲本多核苷酸，以产生编码重组杀虫多肽的重组多核苷酸文库；(b)筛选文库以鉴定编码改善的重组杀虫多肽的重组多核苷酸，所述重组多核苷酸具有比亲本多核苷酸改善的增强的性质；(c)回收编码(b)中鉴定的改善的重组杀虫多肽的重组多核苷酸；和(d)使用步骤(c)中回收的重组多核苷酸作为重复步骤(a)中的多个亲本多核苷酸之一，重复步骤(a)、(b)和(c)。
iii.序列比较
如本文使用的，当关于比对氨基酸序列的特定对使用时，术语“同一性”或“百分比同一性”指氨基酸序列同一性百分比，其通过计数在比对中的相同匹配数目，并且将这样的相同匹配数目除以比对序列的长度来获得。如本文使用的，当关于比对氨基酸序列的特定对使用时，术语“相似性”或“百分比相似性”指从比对中的每个氨基酸对的评分矩阵获得的评分总和除以比对序列的长度。
除非另有说明，否则将通过Needleman-Wunsch总体比对和评分算法(Needleman和Wunsch(1970)J.Mol.Biol.48(3):443-453)计算同一性和相似性，如通过作为EMBOSS软件包的部分分配的“针”程序执行的(Rice，P.等人(2000)EMBOSS:The European MolecularBiology Open Software Suite，Trends in Genetics 16(6):276-277，在其他资源中，可在embnet.org/resource/emboss和emboss.sourceforge.net处从EMBnet获得的版本6.3.1)，使用默认缺口罚分和评分矩阵(用于蛋白质的EBLOSUM62和用于DNA的EDNAFULL)。也可以使用等价程序。“等价程序”指任何序列比较程序，对于所讨论的任何两个序列，当与通过来自EMBOSS 6.3.1版的针所生成的相应比对比较时，其生成具有相同核苷酸残基匹配和相同序列同一性百分比的比对。
另外的数学算法是本领域中已知的并且可以用于比较两个序列。参见例如Karlin和Altschul(1990)Proc.Natl.Acad.Sci.USA 87:2264的算法，如Karlin和Altschul(1993)Proc.Natl.Acad.Sci.USA 90:5873-5877中改动。这种算法被引入Altschul等人(1990)J.Mol.Biol.215:403的BLAST程序内。可以用BLASTN程序(针对核苷酸序列搜索的核苷酸查询)执行BLAST核苷酸搜索，以获得与本发明的杀虫样核酸分子同源的核苷酸序列，或用BLASTX程序(针对蛋白质序列搜索的翻译核苷酸查询)，以获得与本发明的杀虫核酸分子同源的蛋白质序列。可以用BLASTP程序(针对蛋白质序列搜索的蛋白质查询)执行BLAST蛋白质搜索，以获得与本发明的杀虫蛋白质分子同源的氨基酸序列，或用TBLASTN程序(针对翻译的核苷酸序列搜索的蛋白质查询)，以获得与本发明的杀虫蛋白质分子同源的核苷酸序列。为了获得用于比较目的的缺口比对，Gapped BLAST(在BLAST 2.0中)可以如Altschul等人(1997)Nucleic Acids Res.25:3389中所述利用。可替代地，PSI-Blast可以用于执行迭代搜索，其检测分子之间的遥远关系。参见Altschul等人(1997)同上。当利用BLAST、GappedBLAST和PSI-Blast程序时，可以使用相应程序(例如BLASTX和BLASTN)的默认参数。比对也可以通过检查手动执行。
当使用限定的氨基酸取代矩阵(例如，BLOSUM62)、缺口存在罚分和缺口延伸罚分，以便获得对于该对序列可能的最高评分，对两个序列就相似性评分进行比对时，它们是“最佳比对的”。氨基酸取代矩阵及其在量化两个序列之间的相似性中的用途是本领域众所周知的，并且在例如Dayhoff等人(1978)“A model of evolutionary change in proteins.”In“Atlas of Protein Sequence and Structure,”第5卷，Suppl.3(编辑M.O.Dayhoff)，第345-352页.Natl.Biomed.Res.Found.，Washington，D.C.和Henikoff等人(1992)Proc.Natl.Acad.Sci.USA 89:10915-10919中描述。BLOSUM62矩阵经常被用作序列比对方案中的缺省评分取代矩阵。对于在比对的序列之一中引入单个氨基酸缺口而施加缺口存在罚分，对于插入已经开放的缺口内的每个另外的空氨基酸位置施加缺口延伸罚分。由比对在每种序列上开始和结束的氨基酸位置、并且任选地通过在一个或两个序列中插入缺口或多个缺口、以便达到最高的可能评分来确定比对。尽管可以手动完成最佳比对和评分，但通过使用计算机执行的比对算法来促进该过程，所述算法例如缺口BLAST2.0，如Altschul等人(1997)Nucleic Acids Res.25:3389-3402中所述的，并且在美国国家生物技术信息中心网站(National Center for Biotechnology Information Website)(www.ncbi.nlm.nih.gov)上可公开获得。最佳比对(包括多重比对)可以使用例如PSI-BLAST来制备，所述PSI-BLAST可通过www.ncbi.nlm.nih.gov获得，其如Al tschul等人(1997)Nucleic Acids Res.25:3389-3402所述。
关于与参考序列最佳比对的氨基酸序列，氨基酸残基“对应于”在比对中与残基配对的参考序列中的位置。“位置”由数目指示，该数目基于其相对于N末端的位置依次鉴定参考序列中的每个氨基酸。例如，在SEQ ID NO:1中，位置1是L，位置2是S，位置3是I等。当测试序列与SEQ ID NO:1最佳比对时，测试序列中与在位置3处的I比对的残基被说成“对应于SEQ ID NO:1的位置3”。由于在确定最佳比对时必须考虑的缺失、插入、截短、融合等，一般而言，如通过从N末端简单计数确定的测试序列中的氨基酸残基数目不一定与参考序列中其对应位置的数目相同。例如，在其中比对的测试序列中存在缺失的情况下，在缺失位点处没有氨基酸对应于参考序列中的位置。当比对的参考序列中存在插入时，该插入将不对应于参考序列中的任何氨基酸位置。在截短或融合的情况下，在参考序列或比对序列中可以存在不对应于相应序列中的任何氨基酸的氨基酸段。
iv.抗体
还涵盖了针对本发明多肽或其变体或片段的抗体。用于产生抗体的方法是本领域众所周知的(参见例如Harlow和Lane(1988)Antibodies:A Laboratory Manual、ColdSpring Harbor Laboratory、Cold Spring Harbor、N.Y.；和美国专利号4,196,265)。这些抗体可以用于检测和分离毒素多肽的试剂盒中。因此，本公开内容提供了包含抗体的试剂盒，所述抗体与本文所述的多肽、包括例如具有下述序列的多肽特异性结合：SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133,134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212,213、214、215、216、217、218、219、220、221、222、223和/或224。
II.害虫
本文提供的组合物和方法可用于对抗各种害虫。“害虫”包括但不限于昆虫、真菌、细菌、线虫、螨、原生动物病原体、动物寄生性肝吸虫等等。特别感兴趣的害虫是昆虫害虫，特别是对农业植物造成严重损害的昆虫害虫。昆虫害虫包括选自鞘翅目、双翅目、膜翅目、鳞翅目、食毛目、同翅目、半翅目、Orthroptera、缨翅目(Thysanoptera)、革翅目(Dermaptera)、等翅目(Isoptera)、虱目、蚤目(Siphonaptera)、毛翅目(Trichoptera)或线虫的昆虫。在非限制性实施方案中，昆虫害虫可以包括西方玉米根虫，玉米根萤叶甲(Diabrotica virgifera virgifera)；秋粘虫，草地贪夜蛾；科罗拉多马铃薯甲虫，马铃薯甲虫(Leptinotarsa decemlineata)；玉米穗虫，玉米穗虫(在北美相同物种攻击棉花且被称为棉铃虫)；欧洲玉米螟，欧洲玉米螟(Ostrinia nubilalis)；小地老虎，黑切根虫；小菜蛾，小菜蛾(Plutella xylostella)；黍豆毛毛虫，大豆夜蛾(Anticarsia gemmatalis)；西南玉米螟，西南玉米螟(Diatraea grandiosella)；棉铃虫，棉铃虫(在全世界除美国外的其他地区发现)；南绿蝽象，稻绿蝽(Nezara viridula)；绿蝽象，Chinavia halaris；Brownmarmorated蝽象，褐翅蝽象(Halyomorpha halys)；和褐臭蝽，褐臭蝽(Euschistusservus)、大豆褐蝽(Euschistus heros)(新热带棕蝽象和/或大豆蝽象)；红蝽(Piezodorusguildinii)(红带蝽象)；Dichelops melacanthus(无通用名)和/或Dichelops furcatus(无通用名)；蚜虫，例如大豆蚜虫。在其他实施方案中，害虫包含线虫，包括但不限于：根结线虫(Meloidogyne hapla)(北方根结线虫)；象耳豆根结线虫(Meloidogyneenterolobii)、花生根结线虫(Meloidogyne arenaria)(花生根结线虫)；和/或爪哇根结线虫(Meloidogyne javanica)。
如本文使用的，术语“害虫”指昆虫和其他类似的害虫，例如蜱螨亚纲的那些，包括但不限于螨和蜱。本发明的昆虫害虫包括但不限于鳞翅目昆虫，例如小蜡螟(Achoroiagrisella)、黑头长翅卷蛾(Acleris gloverana)、Acleris variana、苹果小卷叶蛾(Adoxophyes orana)、黑切根虫、棉叶波纹夜蛾(Alabama argillacea)、秋尺蠖(Alsophilapometaria)、脐橙螟蛾(Amyelois transitella)、地中海粉螟(Anagasta kuehniella)、桃枝麦蛾(Anarsia lineatella)、橙纹犀额蛾(Anisota senatoria)、柞蚕(Antheraeapernyi)、大豆夜蛾、黄卷蛾属物种(Archips sp.)、带卷蛾属物种(Argyrotaenia sp.)、Athetis mindara、家蚕(Bombyx mori)、棉潜蛾(Bucculatrix thurberiella)、粉斑螟蛾(Cadra cautella)、色卷蛾属物种(Choristoneura sp.)、Cochylls hospes、苜蓿黄蝶(Colias eurytheme)、米蛾(Corcyra cephalonica)、Cydia latiferreanus、苹果蠹蛾、古月桃黄蝶(Datana integerrima)、Dendrolimus sibericus、Desmiafeneralis、甜瓜绢野螟(Diaphania hyalinata)、黄瓜绢野螟(Diaphania nitidalis)、西南玉米螟、小蔗螟(Diatraea saccharalis)、白尺蠖蛾(Ennomos subsignaria)、墨西哥稻螟(Eoreumaloftini)、烟草粉螟(Esphestia elutella)、Erannis tilaria、Estigmene acrea、Euliasalubricola、Eupocoellia ambiguella、女贞细卷蛾(Eupoecilia ambiguella)、褐尾蠹(Euproctis chrysorrhoea)、暗缘地老虎(Euxoa messoria)、大蜡螟(Galleriamellonella)、梨小食心虫(Grapholita molesta)、黑拟蛉蛾(Harrisina americana)、Helicoverpa subflexa、玉米穗虫、烟芽夜蛾、Hemileuca oliviae、向日葵斑螟(Homoeosoma electellum)、美国白蛾(Hyphantia cunea)、番茄蠹蛾(Keiferialycopersicella)、Lambdina fiscellaria fiscellaria、Lambdina fiscellarialugubrosa、柳毒蛾(Leucoma salicis)、葡萄花翅小卷蛾(Lobesia botrana)、草地螟(Loxostege sticticalis)、舞毒蛾(Lymantria dispar)、Macalla thyrisalis、天幕毛虫属物种(Malacosoma sp.)、甘蓝夜蛾(Mamestra brassicae)、蓓带夜蛾(Mamestraconfigurata)、番茄天蛾(Manduca quinquemaculata)、烟草天蛾(Manduca sexta)、豆荚野螟(Maruca testulalis)、Melanchra picta、冬尺蛾(Operophtera brumata)、毒蛾属物种(Orgyia sp.)、欧洲玉米螟、春尺蠖(Paleacrita vernata)、美洲大芷凤蝶(Papiliocresphontes)、红棉铃虫(Pectinophora gossypiella)、加州槲蛾(Phryganidiacalifornica)、斑幕潜叶蛾(Phyllonorycter blancardella)、暗脉菜粉蝶(Pieris napi)、菜粉蝶(Pieris rapae)、苜蓿绿夜蛾(Plathypena scabra)、Platynota flouendana、荷兰石竹小卷蛾(Platynota stultana)、Platyptilia carduidactyla、印度谷螟(Plodiainterpunctella)、小莱蛾(Plutella xylostella)、Pontia protodice、美洲粘虫(Pseudaletia unipuncta)、Pseudoplasia includens、杂食尺蠖(Sabulodes aegrotata)、红抚天社蛾(Schizura concinna)、麦蛾(Sitotroga cerealella)、苹果小卷叶蛾(Spilonta ocellana)、斜纹夜蛾属物种(Spodoptera sp.)、Thaurnstopoea pityocampa、衣蛾(Tinsola bisselliella)、粉纹夜蛾(Trichoplusia hi)、温室结网野螟(Udearubigalis)、Xylomyges curiails和苹果巢蛾(Yponomeuta padella)。
昆虫害虫还包括选自双翅目、膜翅目、鳞翅目、食毛目、同翅目、半翅目、Orthroptera、缨翅目、革翅目、等翅目、虱目、蚤目、毛翅目、鞘翅目的昆虫。
对于主要作物的本发明的害虫包括但不限于：玉蜀黍：欧洲玉米螟，欧洲玉米螟；黑切根虫，黑切根虫；玉米穗虫，玉米穗虫；草地贪夜蛾，秋粘虫；西南玉米螟，西南玉米螟；小玉米茎蛀虫(Elasmopalpus lignosellus)，小玉米茎蛀虫；小蔗螟，甘蔗螟虫；西方玉米根虫，例如玉米根萤叶甲；北方玉米根虫，例如Diabrotica longicornis barberi；南方玉米根虫，例如Diabrotica undecimpunctata howardi；梳爪叩甲属物种(Melanotus spp.)，线虫；北方圆头犀金龟(Cyclocephala borealis)，北方假面金龟子(蛴螬)；南方圆头犀金龟(Cyclocephala immaculata)，南方假面金龟子(蛴螬)；日本弧丽金龟，日本金龟子；玉米跳甲(Chaetocnema pulicaria)，玉米跳甲；玉米象虫(Sphenophorus maidis)，玉米象虫；玉米蚜(Rhopalosiphum maidis)，玉米蚜；玉蜀黍根蚜(Anuraphis maidiradicis)，玉米根蚜；Blissus leucopterus leucopterus，麦小蝽；Melanoplus femurrubrum，红腿蚱蜢；迁徒蚱蜢(Melanoplus sanguinipes)，迁徒蚱蜢；灰地种蝇(Hylemya platura)，种蝇；美洲黍潜叶蝇(Agromyza parvicornis)，玉米斑潜叶蝇；玉米黄呆蓟马(Anaphothripsobscrurus)，草蓟马；红火蚁(Solenopsis milesta)，盗蚁；二斑叶螨(Tetranychusurticae)，二斑叶螨；高粱：玉米斑点螟(Chilo partellus)，高粱螟；草地贪夜蛾，秋粘虫；玉米穗虫，玉米穗虫；小玉米茎蛀虫，小玉米茎蛀虫；粒肤地老虎(Feltia subterranea)，颗粒地老虎；Phyllophaga crinita，蛴螬；伪金针虫属(Eleodes)，宽胸叩头虫属(Conoderus)和Aeolus spp.，线虫；谷叶甲虫(Oulema melanopus)，谷物叶甲；玉米跳甲，玉米跳甲；玉米象虫，玉米象虫；玉米蚜，玉米蚜；蔗黄伪毛蚜(Sipha flava)，黄甘蔗蚜虫；麦小蝽，例如Blissus leucopterus leucopterus；高粱瘿蚊(Contarinia sorghicola)，高粱瘿蚊；朱砂叶螨(Tetranychus cinnabarinus)，朱砂叶螨；二斑叶螨，二斑叶螨；小麦：Pseudaletiaunipunctata，粘虫；草地贪夜蛾；秋粘虫；小玉米茎蛀虫，小玉米茎蛀虫；谷叶甲虫，谷物叶甲；三叶草叶象，三叶草叶象；南方玉米根虫，例如Diabrotica undecimpunctata howardi；俄罗斯小麦蚜虫；麦二叉蚜(Schizaphis graminum)，麦二叉蚜；麦长管蚜(Macrosiphumavenae)，麦长管蚜；Melanoplus femurrubrum，红腿蚱蜢；殊种蚱蜢(Melanoplusdifferentialis)，殊种蚱蜢；迁徒蚱蜢，迁徒蚱蜢；小麦瘿蚊(Mayetiola destructor)，小麦瘿蝇；麦红吸浆虫(Sitodiplosis mosellana)，小麦吸浆虫；美州麦杆蝇(Meromyzaamericana)，麦杆蝇；麦种蝇(Hylemya coarctata)，麦种蝇；烟草褐花蓟马(Frankliniellafusca)，烟草蓟马；麦茎蜂(Cephus cinctus)，小麦茎蜂；小麦曲叶螨(Aceria tulipae)，小麦卷叶螨；向日葵：向曰葵茎象甲(Cylindrocupturus adspersus)，向日葵茎象甲；红色种子象(Smicronyx fulus)，红色向日葵种子象鼻虫；灰色种子象(Smicronyx sordidus)，灰色向日葵种子象鼻虫；向日葵食芽蛾(Suleima helianthana)，向日葵芽蛾；向日葵斑螟，向日葵螟；向日葵叶甲(Zygogramma exclamationis)，向日葵甲虫；胡萝卜金龟(Bothyrusgibbosus)，胡萝卜甲虫；向日葵籽瘿蚊(Neolasioptera murtfeldtiana)，向日葵籽瘿蚊；棉花：烟芽夜蛾，烟夜蛾；玉米穗虫，玉米穗虫；甜菜夜蛾(Spodoptera exigua)，甜菜夜蛾；红棉铃虫，红铃虫；棉铃象鼻虫，例如棉铃象甲(Anthonomus grandis)；棉蚜(Aphisgossypii)，棉蚜；棉盲蝽(Pseudatomoscelis seriatus)，棉盲蝽；纹翅粉虱(Trialeurodesabutilonea)，纹翅粉虱；美国牧草盲蝽(Lygus lineolaris)，牧草盲蝽；Melanoplusfemurrubrum，红腿蚱蜢；殊种蚱蜢，殊种蚱蜢；烟蓟马(Thrips tabaci)，葱蓟马；烟草褐花蓟马，烟草蓟马；朱砂叶螨，朱砂叶螨；二斑叶螨，二斑叶螨；稻：小蔗螟，甘蔗螟虫；草地贪夜蛾，秋粘虫；玉米穗虫，玉米穗虫；葡萄肖叶甲(Colaspis brunnea)，葡萄肖叶甲；稻水象(Lissorhoptrus oryzophilus)，稻水象甲；米象(Sitophilus oryzae)，米象；二条黑尾叶蝉(Nephotettix nigropictus)，稻叶蝉；麦小蝽，例如Blissus leucopterusleucopterus；拟绿蝽(Acrosternum hilare)，绿蝽；大豆：大豆夜蛾，大豆尺蠖；大豆夜蛾，黍豆毛毛虫；苜蓿绿夜蛾，绿夜蛾；欧洲玉米螟，欧洲玉米螟；黑切根虫，黑切根虫；甜菜夜蛾，甜菜夜蛾；烟芽夜蛾，烟夜蛾；玉米穗虫，玉米穗虫；墨西哥豆瓢虫(Epilachnavarivestis)，墨西哥豆甲虫；桃蚜(Myzus persicae)，绿桃蚜虫；马铃薯小绿叶蝉(Empoasca fabae)，马铃薯叶蝉；拟绿蝽，绿蝽；Melanoplus femurrubrum，红腿蚱蜢；殊种蚱蜢，殊种蚱蜢；灰地种蝇，种蝇；大豆蓟马(Sericothrips variabilis)，大豆蓟马；烟蓟马，葱蓟马；土耳其斯坦叶螨(Tetranychus turkestani)，草莓蜘蛛螨；二斑叶螨，二斑叶螨；大麦：欧洲玉米螟，欧洲玉米螟；黑切根虫，黑切根虫；麦二叉蚜，麦二叉蚜；麦小蝽，例如Blissus leucopterus leucopterus；拟绿蝽，绿蝽；褐臭蝽，褐臭蝽；Jylemya platura，种蝇；小麦瘿蚊，小麦瘿蝇；麦岩螨，棕色小麦螨；油籽油菜：甘蓝蚜(Vrevicorynebrassicae)，卷心菜蚜虫；十字花科跳甲(Phyllotreta cruciferae)，十字花科跳甲；黄曲条跳甲(Phyllotreta striolata)，黄条跳甲；大豆淡足跳甲(Phyllotreta nemorum)，条纹萝卜跳甲；油菜露尾甲(Meligethes aeneus)，油菜甲虫；以及花粉甲虫Meligethesrufimanus、Meligethes nigrescens、Meligethes canadianus和Meligethesviridescens；马铃薯：马铃薯甲虫，科罗拉多马铃薯甲虫。
本发明提供的方法和组合物可以有效抗半翅目，例如草盲蝽(Lygus hesperus)、美国牧草盲蝽、牧草盲蝽(Lygus pratensis)、长毛草盲蝽(Lygus rugulipennis Popp)、Lygus pabulinus、土豆盲蝽(Calocoris norvegicus)、泛奥盲蝽(Orthops compestris)、苹果盲蝽(Plesiocoris rugicollis)、Cyrtopeltis modestus、黑斑烟盲蝽(Cyrtopeltisnotatus)、白斑盲蝽(Spanagonicus albofasciatus)、Diaphnocoris chlorinonis、Labopidicola allii、棉盲蝽(Pseudatomoscelis seriatus)、苜蓿盲蝽(Adelphocorisrapidus)、四线盲蝽(Poecilocapsus lineatus)、麦长蝽(Blissus leucopterus)、小长蝽(Nysius ericae)、Nysius raphanus、褐臭蝽、稻绿蝽、扁盾蝽属(Eurygaster)、缘蝽科(Coreidae)、红蝽科(Pyrrhocoridae)、Tinidae、负子蝽科(Blostomatidae)、猎蝽科(Reduviidae)和臭虫科(Cimicidae)。感兴趣的害虫还包括咖啡豆象(Araecerusfasciculatus)，咖啡豆象；大豆象(Acanthoscelides obtectus)，大豆象；蚕豆象(Bruchusrufmanus)，蚕豆象；豌豆象(Bruchus pisorum)，豌豆象；墨西哥豆象(Zabrotessubfasciatus)，墨西哥豆象；黄瓜叶甲(Diabrotica balteata)，带状黄瓜甲虫；菜豆萤叶甲(Cerotoma trifurcata)，豆叶甲；玉米根叶甲，墨西哥玉米根虫；黄瓜跳甲，马铃薯跳甲；甘薯跳甲(Chaetocnema confinis)，甘薯跳甲；苜蓿叶象(Hypera postica)，苜蓿象甲；苹果花象(Anthonomus quadrigibbus)，苹果象鼻虫；豆茎象(Sternechus paludatus)，豆茎象鼻虫；埃及苜蓿叶象(Hypera brunnipennis)，埃及苜蓿象甲；谷象(Sitophilusgranaries)，谷象；葡萄象(Craponius inaequalis)，葡萄象鼻虫；玉米象(Sitophiluszeamais)，玉米象；李象(Conotrachelus nenuphar)，李象鼻虫；西印度群岛甘薯象甲，西印度甘薯象甲；紫绒鲍角金龟(Maladera castanea)，亚洲花园甲虫；欧洲金龟(Rhizotrogusmajalis)，欧洲金龟子；蔷薇刺金龟(Macrodactylus subspinosus)，玫瑰金龟子；杂拟谷盗(Tribolium confusum)，杂拟谷盗；黑粉虫(Tenebrio obscurus)，黑粉虫；赤拟谷盗(Tribolium castaneum)，赤拟谷盗；黄粉虫(Tenebrio molitor)，黄粉虫。
线虫包括寄生线虫例如根节、孢囊和病变线虫，包括异皮线虫属物种(Heteroderaspp.)、根结线虫属物种(Meloidogyne spp.)和球异皮线虫属物种(Globodera spp.)；特别是孢囊线虫的成员，包括但不限于大豆孢囊线虫(Heterodera glycines)(大豆孢囊线虫)；甜菜孢囊线虫(Heterodera schachtii)(甜菜孢囊线虫)；禾谷孢囊线虫(Heteroderaavenae)(禾谷孢囊线虫)；以及马铃薯金线虫(Globodera rostochiensis)和马铃薯白线虫(Globodera pailida)(马铃薯孢囊线虫)。病变线虫包括短体线虫属物种(Pratylenchusspp.)。
可以在早期发育阶段(例如幼虫或其他未成熟形式)对昆虫害虫测试本发明组合物的杀虫活性。昆虫可以在完全黑暗中在约20℃至约30℃和约30％至约70％相对湿度下饲养。生物测定可以如Czapla和Lang(1990)J.Econ.Entomol.83(6):2480-2485中所述执行。还参见本文的实验部分。
III.表达盒
可以在表达盒中提供编码本文提供的杀虫蛋白质的多核苷酸，用于在目的生物中表达。所述盒将包括与编码本文提供的杀虫多肽的多核苷酸可操作地连接的5'和3'调节序列，其允许多核苷酸的表达。所述盒可以另外含有待共转化到生物内的至少一种另外的基因或遗传元件。当包括另外的基因或元件时，各组分可操作地连接。可替代地，可以在多个表达盒上提供另外的基因或元件。这样的表达盒有多个限制性位点和/或重组位点，用于插入多核苷酸以处于调节区的转录调节之下。表达盒可以另外含有选择标记基因。
表达盒将在5'-3'转录方向上包括转录和翻译起始区(即启动子)、本发明的杀虫多核苷酸、以及在目的生物即植物或细菌中起作用的转录和翻译终止区(即终止区)。本发明的启动子能够指导或驱动编码序列在宿主细胞中的表达。调节区(即启动子、转录调节区和翻译终止区)对于宿主细胞或彼此之间可以是内源的或异源的。如本文使用的，提及序列时“异源”是源自外来物种的序列，或者如果来自相同物种，则通过有意的人为干预而在组成和/或基因组基因座中从其天然形式实质上修饰而来。如本文使用的，嵌合基因包含可操作地连接至对编码序列异源的转录起始区的编码序列。
方便的终止区可从根瘤土壤杆菌(A.tumefaciens)的Ti质粒获得，例如章鱼碱合酶和胭脂碱合酶终止区。还参见Guer ineau等人(1991)Mol.Gen.Genet.262:141-144；Proudfoot(1991)Cell64:671-674；Sanfacon等人(1991)Genes Dev.5:141-149；Mogen等人(1990)Plant Cell 2:1261-1272；Munroe等人(1990)Gene91:151-158；Ballas等人(1989)Nucleic Acids Res.17:7891-7903；和Joshi等人(1987)Nucleic Acids Res.15:9627-9639。
另外的调节信号包括但不限于转录开始起始位点、操纵子、激活子、增强子、其他调节元件、核糖体结合位点、起始密码子、终止信号等等。参见例如，美国专利号5,039,523和4,853,331；EP0480762A2；Sambrook等人(1992)Molecular Cloning:A LaboratoryManual，编辑Maniatis等人(Cold Spring Harbor Laboratory Press，Cold SpringHarbor，N.Y.)，下文称为“Sambrook 11”；Davis等人，编辑(1980)Advanced BacterialGenetics(Cold Spring Harbor Laboratory Press)，Cold Spring Harbor，N.Y.，以及其中引用的参考文献。
在制备表达盒时，可以操纵各种DNA片段，以便提供以正确取向并且适当地正确读码框的DNA序列。为此，衔接子或接头可以用于连接DNA片段，或者可以涉及其他操纵以提供方便的限制性位点、去除多余的DNA、去除限制性位点等等。为此目的，可以涉及体外诱变、引物修复、限制性、退火、再取代例如转换和颠换。
在本发明的实践中可以使用许多启动子。可以基于所需结果来选择启动子。核酸可以与组成型、诱导型、组织偏好型或其他启动子组合，用于在目的生物中表达。参见例如，WO 99/43838以及美国专利号8,575,425；7,790,846；8,147,856；8,586832；7,772,369；7,534,939；6,072,050；5,659,026；5,608,149；5,608,144；5,604,121；5,569,597；5,466,785；5,399,680；5,268,463；5,608,142；和6,177,611中所述的启动子；各自通过引用并入本文。
为了在植物中表达，组成型启动子还包括CaMV 35S启动子(Odell等(1985)Nature313:810-812)；水稻肌动蛋白(McElroy等人(1990)Plant Cell 2:163-171)；遍在蛋白(Christensen等人(1989)Plant Mol.Biol.12:619-632和Christensen等人(1992)PlantMol.Biol.18:675-689)；pEMU(Last等人(1991)Theor.Appl.Genet.81:581-588)；MAS(Velten等人(1984)EMBO J.3:2723-2730)。诱导型启动子包括驱动发病机制相关蛋白(PR蛋白)表达的那些启动子，所述PR蛋白在被病原体感染后被诱导。参见例如Redolfi等人(1983)Neth.J.Plant Pathol.89:245-254；Uknes等人(1992)Plant Cell 4:645-656；和Van Loon(1985)Plant Mol.Virol.4:111-116；和WO 99/43819，引入本文作为参考。也可以使用在病原体感染位点处或附近局部表达的启动子(Marineau等人(1987)PlantMol.Biol.9:335-342；Matton等人(1989)Molecular Plant-Microbe Interactions 2:325-331；Somsisch等人(1986)Proc.Natl.Acad.Sci.USA 83:2427-2430；Somsisch等人(1988)Mol.Gen.Genet.2:93-98；和Yang(1996)Proc.Natl.Acad.Sci.USA 93:14972-14977；Chen等人(1996)Plant J.10:955-966；Zhang等人(1994)Proc.Natl.Acad.Sci.USA91:2507-2511；Warner等人(1993)Plant J.3:191-201；Siebertz等人(1989)Plant Cell1:961-968；Cordero等人(1992)Physiol.Mol.Plant Path.41:189-200；美国专利号5,750,386(线虫诱导型)；以及其中引用的参考文献)。
创伤诱导型启动子可以用于本发明的构建体中。此类创伤诱导型启动子包括pinII启动子(Ryan(1990)Ann.Rev.Phytopath.28:425-449；Duan等人(1996)NatureBiotechnology 14:494-498)；wun1和wun2(美国专利5,428,148)；win1和win2(Stanford等人(1989)Mol.Gen.Genet.215:200-208)；系统素(McGurl等人(1992)Science 225:1570-1573)；WIP1(Rohmeier等人(1993)Plant Mol.Biol.22:783-792；Eckelkamp等人(1993)FEBS Letters 323:73-76)；MPI基因(Corderok等人(1994)Plant J.6(2):141-150)；等等，通过引用并入本文。
用于本发明的组织偏好型启动子包括在下述中阐述的那些：Yamamoto等人(1997)Plant J.12(2):255-265；Kawamata等人(1997)Plant Cell Physiol.38(7):792-803；Hansen等人(1997)Mol.Gen Genet.254(3):337-343；Russell等人(1997)TransgenicRes.6(2):157-168；Rinehart等人(1996)Plant Physiol.112(3):1331-1341；Van Camp等人(1996)Plant Physiol.112(2):525-535；Canevascini等人(1996)Plant Physiol.112(2):513-524；Yamamoto等人(1994)Plant Cell Physiol.35(5):773-778；Lam(1994)Results Probl.Cell Differ.20:181-196；Orozco等人(1993)Plant Mol Biol.23(6):1129-1138；Matsuoka等人(1993)Proc Natl.Acad.Sci.USA 90(20):9586-9590；和Guevara-Garcia等人(1993)Plant J.4(3):495-505。
叶偏好的启动子包括在下述中阐述的那些：Yamamoto等人(1997)Plant J.12(2):255-265；Kwon等人(1994)Plant Physiol.105:357-67；Yamamoto等人(1994)Plant CellPhysiol.35(5):773-778；Gotor等人(1993)Plant J.3:509-18；Orozco等人(1993)PlantMol.Biol.23(6):1129-1138；和Matsuoka等人(1993)Proc.Natl.Acad.Sci.USA 90(20):9586-9590。
根偏好的启动子是已知的，并且包括下述中的那些：Hire等人(1992)PlantMol.Biol.20(2):207-218(大豆根特异性谷氨酰胺合成酶基因)；Kel ler和Baumgartner(1991)Plant Cell 3(10):1051-1061(根特异性控制元件)；Sanger等人(1990)PlantMol.Biol.14(3):433-443(根瘤土壤杆菌的甘露碱合成酶(MAS)基因；和Miao等人(1991)Plant Cell 3(1):11-22(胞质谷氨酰胺合成酶(GS))；Bogusz等人(1990)Plant Cell 2(7):633-641；Leach和Aoyagi(1991)Plant Science(Limerick)79(1):69-76(rolC和rolD)；Teeri等人(1989)EMBO J.8(2):343-350；Kuster等人(1995)Plant Mol.Biol.29(4):759-772(VfENOD-GRP3基因启动子)；和Capana等人(1994)Plant Mol.Biol.25(4):681-691(rolB启动子)。也参见美国专利号5,837,876；5,750,386；5,633,363；5,459,252；5,401,836；5,110,732；和5,023,179。
“种子偏好型”启动子包括“种子特异性”启动子(在种子发育过程中活跃的那些启动子，例如种子贮藏蛋白的启动子)以及“种子萌发”启动子(在种子萌发过程中活跃的那些启动子)两者。参见Thompson等人(1989)BioEssays 10:108。种子偏好型启动子包括但不限于Cim1(细胞分裂素诱导的信息)；cZ19B1(玉蜀黍19kDa玉米醇溶蛋白)；milps(肌醇-1-磷酸合酶)(参见WO 00/11177和美国专利号6,225,529)。γ-玉米醇溶蛋白是胚乳特异性启动子。球蛋白1(Glb-1)是代表性的胚特异性启动子。对于双子叶植物，种子特异性启动子包括但不限于豆β-菜豆球蛋白、napin、β-伴大豆球蛋白、大豆凝集素、十字花科蛋白等等。对于单子叶植物，种子特异性启动子包括但不限于玉蜀黍15kDa玉米醇溶蛋白、22kDa玉米醇溶蛋白、27kDa玉米醇溶蛋白、γ-玉米醇溶蛋白、蜡质、收缩蛋白(shrunken)1、收缩2、球蛋白1等。也参见WO 00/12733，其中公开了来自end1和end2基因的种子偏好型启动子。
为了在细菌宿主中表达，在细菌中起作用的启动子是本领域众所周知的。这样的启动子包括任何已知的晶体蛋白质基因启动子，包括本发明的任何杀虫蛋白质的启动子，以及对于苏云金芽孢杆菌Σ因子特异性的启动子。可替代地，诱变的或重组的晶体蛋白质编码基因启动子可以被重组改造并且用于促进本文公开的新型基因区段的表达。
表达盒还可以包含用于选择经转化的细胞的可选择标记物基因。可选择标记物基因用于选择经转化的细胞或组织。标记物基因包括编码抗生素抗性的基因，例如编码新霉素磷酸转移酶II(NEO)和潮霉素磷酸转移酶(HPT)的基因，以及赋予对除草剂化合物如草铵膦、溴苯腈、咪唑啉酮和2,4-二氯苯氧乙酸(2,4-D)的抗性的基因。另外的可选择标记物是已知的，并且均可以用于本发明的实践中。参见例如通过引用整体并入本文的于2015年12月18日提交的PCT/US2015/066648，其公开了可以用作可选择标记物的草铵膦抗性序列。
IV.方法、宿主细胞和植物细胞
如所指出的，包含编码杀虫蛋白质或其活性变体或片段的核苷酸序列的DNA构建体可以用于转化感兴趣的植物或感兴趣的其他生物。用于转化的方法涉及将核苷酸构建体引入植物内。“引入”是指将核苷酸构建体以这样的方式引入植物或其他宿主细胞，使得构建体进入植物细胞或宿主细胞的内部。本发明的方法不需要将核苷酸构建体引入植物或宿主细胞的特定方法，只要核苷酸构建体进入植物或宿主生物的至少一个细胞的内部即可。用于将核苷酸构建体引入植物和其他宿主细胞内的方法是本领域已知的，包括但不限于稳定转化方法、瞬时转化方法和病毒介导的方法。
所述方法产生经转化的生物，例如植物，包括完整植物，以及植物器官(例如叶、茎、根等)、种子、植物细胞、繁殖体、胚及其后代。植物细胞可以是分化的或未分化的(例如愈伤组织、悬浮培养细胞、原生质体、叶细胞、根细胞、韧皮部细胞、花粉)。
“转基因植物”或“经转化的植物”或“稳定转化的”植物或细胞或组织指已掺入或整合了编码本发明的至少一种杀虫多肽的多核苷酸的植物。已认识到其他外源或内源核酸序列或DNA片段也可以掺入该植物细胞内。土壤杆菌和生物弹道介导的转化仍然是两种主要采用的方法。然而，可以通过感染、转染、显微注射、电穿孔、微粒轰击、生物弹道或粒子轰击、电穿孔、二氧化硅/碳纤维、超声介导、PEG介导、磷酸钙共沉淀、聚阳离子DMSO技术、DEAE葡聚糖程序、Agro和病毒介导(Caulimoriviruses、双生病毒、RNA植物病毒)、脂质体介导等等来执行。
转化方案以及用于将多肽或多核苷酸序列引入植物内的方案可以根据靶向用于转化的植物或植物细胞的类型(即单子叶或双子叶植物)而变化。转化方法是本领域已知的，包括美国专利号：8,575,425；7,692,068；8,802,934；7,541,517中所述的那些；所述美国专利号各自通过引用并入本文。还参见Rakoczy-Trojanowska，M.(2002)Cell Mol BiolLett.7:849-858；Jones等人(2005)Plant Methods1:5；Rivera等人(2012)Physics ofLife Reviews 9:308-345；Bartlett等人(2008)Plant Methods 4:1-12；Bates，G.W.(1999)Methods in Molecular Biology 111:359-366；Binns和Thomashow(1988)AnnualReviews in Microbiology 42:575-606；Christou，P.(1992)The Plant Journal 2:275-281；Christou，P.(1995)Euphytica 85:13-27；Tzfira等人(2004)TRENDS in Genetics20:375-383；Yao等人(2006)Journal of Experimental Botany57:3737-3746；Zupan和Zambryski(1995)Plant Physiology107:1041-1047；Jones等人(2005)Plant Methods 1:5。
转化可导致核酸稳定或瞬时掺入细胞内。“稳定转化”意指引入宿主细胞内的核苷酸构建体整合到宿主细胞的基因组内，并且能够被其后代继承。“瞬时转化”意指将多核苷酸被引入宿主细胞内，并且不整合到宿主细胞的基因组内。
用于叶绿体转化的方法是本领域已知的。参见例如，Svab等人(1990)Proc.Natl.Acad.Sci.USA 87:8526-8530；Svab和Maliga(1993)Proc.Natl.Acad.Sci.USA90:913-917；Svab和Maliga(1993)EMBO J.12:601-606。该方法依赖于含有可选择标记物的DNA的粒子枪递送，并且通过同源重组将DNA靶向质体基因组。另外，质体转化可以通过由核编码的和质体定向的RNA聚合酶的组织偏好表达反式激活沉默的质体携带的转基因来实现。这种系统已在McBride等人(1994)Proc.Natl.Acad.Sci.USA 91:7301-7305中得到报道。
已转化的细胞可以按照常规方式生长成植物。参见例如，McCormick等人(1986)Plant Cell Reports 5:81-84。然后可以使这些植物生长，并且用相同的转化品系或不同品系授粉，并且鉴定具有所需表型特征的组成型表达的所得到的杂种。可以生长两代或更多代以确保所需表型特征的表达得到稳定维持和继承，然后收获种子以确保已实现了所需表型特征的表达。以这种方式，本发明提供了具有稳定整合到其基因组内的本发明的核苷酸构建体(例如本发明的表达盒)的经转化的种子(也称为“转基因种子”)。
在具体的实施方案中，本文提供的序列可以被靶向宿主细胞或植物细胞的基因组内的特定位点。此类方法包括但不限于针对目的植物基因组序列设计的大范围核酸酶(meganucleases)(D’Halluin等人2013Plant Biotechnol J)；CRISPR-Cas9、TALEN和用于基因组的精确编辑的其他技术(Feng等人(2013)Cell Research 23:1229-1232；Podevin等人(2013)Trends Biotechnology，在线公开；Wei等人(2013)J Gen Genomics；Zhang等人(2013)WO 2013/026740)；Cre-lox位点特异性重组(Dale等人(1995)Plant J 7:649-659；Lyznik等人(2007)Transgenic Plant J 1:1-9；FLP-FRT重组(Li等人(2009)PlantPhysiol 151:1087-1095)；Bxb1介导的整合(Yau等人Plant J(2011)701:147-166)；锌指介导的整合(Wright等人(2005)Plant J 44:693-705)；Cai等人(2009)Plant Mol Biol69:699-709)；和同源重组(Lieberman-Lazarovich和Levy(2011)Methods Mol Biol 701:51-65)；Puchta(2002)Plant Mol Biol48:173-182)。
本文提供的序列可以用于转化任何植物物种，包括但不限于单子叶植物和双子叶植物。感兴趣的植物的实例包括但不限于玉米(玉蜀黍)、高粱、小麦、向日葵、番茄、十字花科植物、辣椒、马铃薯、棉花、稻、大豆、甜菜、甘蔗、烟草、大麦和油菜、芸苔属物种(Brassicasp.)、苜蓿、黑麦、小米、红花、花生、甘薯、木薯(cassaya)、咖啡、椰子、菠萝、柑橘树、可可、茶、香蕉、鳄梨、无花果、番石榴、芒果、橄榄、木瓜、腰果、澳洲坚果、杏仁、燕麦、蔬菜、观赏植物和针叶树。
蔬菜包括但不限于番茄，莴苣，绿豆，利马豆，豌豆和黄瓜属(Curcumis)的成员，例如黄瓜、哈密瓜和甜瓜。观赏植物包括但不限于杜鹃花、绣球花、芙蓉、玫瑰、郁金香、水仙、矮牵牛、康乃馨、一品红和菊花。优选地，本发明的植物是作物植物(例如玉蜀黍、高粱、小麦、向日葵、番茄、十字花科植物、辣椒、马铃薯、棉花、稻、大豆、甜菜、甘蔗、烟草、大麦、油籽油菜等)。
如本文使用的，术语植物包括植物细胞、植物原生质体、由其可以再生植物的植物细胞组织培养物、植物愈伤组织、植物团块和在植物或植物部分中完整的植物细胞例如胚、花粉、胚珠、种子、叶、花、枝、果实、果仁、穗、穗轴、外壳、茎、根、根尖、花药等等。谷类意指通过商业种植者为了除生长或繁殖物种外的目的而生产的成熟种子。再生植物的后代、变体和突变体也包括在本发明的范围内，条件是这些部分包含所引入的多核苷酸。进一步提供了保留了本文公开的序列的经加工的植物产物或副产物，包括例如豆粕。
在另一个实施方案中，编码杀虫蛋白质的基因可以用于转化昆虫致病生物。这样的生物包括杆状病毒、真菌、原生动物、细菌和线虫。可以选择已知占据一种或多种目的作物的“植物圈”(叶面、叶际、根际和/或根面(rhizoplana))的微生物宿主。选择这些微生物以便能够在特定的环境中与野生型微生物成功竞争，提供表达杀虫蛋白质的基因的稳定维持和表达，并且期望地提供杀虫剂免于环境降解和灭活的更好保护。
这样的微生物包括古细菌、细菌、藻类和真菌。特别感兴趣的是微生物，例如细菌，例如芽孢杆菌属、假单胞菌属(Pseudomonas)、欧文氏菌属(Erwinia)、沙雷氏菌属(Serratia)、克雷伯氏菌属(Klebsiella)、黄单胞菌属(Xanthomonas)、链霉菌属(Streptomyces)、根瘤菌属(Rhizobium)、红假单胞菌属(Rhodopseudomonas)、Methylius、土壤杆菌属(Agrobacterium)、乙杆菌属(Acetobacter)、乳杆菌属(Lactobacillus)、节杆菌属(Arthrobacter)、固氮菌属(Azotobacter)、明串珠菌属(Leuconostoc)和产碱菌属(Alcaligenes)。真菌包括酵母，例如酵母属(Saccharomyces)、隐球酵母属(Cryptococcus)、克鲁维酵母属(Kluyveromyces)、掷孢酵母属(Sporobolomyces)、红酵母属(Rhodotorula)和短梗霉属(Aureobasidium)。特别感兴趣的是这些植物圈细菌物种，如丁香假单胞菌(Pseudomonas syringae)、铜绿假单胞菌(Pseudomonas aeruginosa)、荧光假单胞菌(Pseudomonas fluorescens)、粘质沙雷氏菌(Serratia marcescens)、木乙杆菌(Acetobacter xylinum)、土壤杆菌属、球形红假单胞菌(Rhodopseudomonas spheroides)、野油菜黄单胞菌(Xanthomonas campestris)、苜蓿根瘤菌(Rhizobiummelioti)、真养产碱杆菌(Alcaligenes entrophus)、木质棒状杆菌(Clavibacter xyli)和维氏固氮菌(Azotobacter vinlandir)，以及植物圈酵母物种例如深红酵母(Rhodotorula rubra)、粘红酵母(R.glutinis)、海滨红酵母(R.marina)、橙红酵母(R.aurantiaca)、浅白隐球酵母(Cryptococcus albidus)、流散假丝酵母(C.diffluens)、生防酵母(C.laurentii)、罗斯酵母(Saccharomyces rosei)、S.pretoriensis、酿酒酵母(S.cerevisiae)、Sporobolomycesrosues、S.odorus、Kluyveromyces veronae、禾口出芽短梗霉(Aureobasidiumpollulans)、苏云金芽孢杆菌、大肠杆菌(Escherichia coli)、枯草芽孢杆菌等等。
示例性的原核生物(革兰氏阴性和革兰氏阳性两者)包括肠杆菌科(Enterobacteriaceae)，例如埃希氏菌属(Escherichia)、欧文氏菌属、志贺氏菌属(Shigella)、沙门氏菌属(Salmonella)和变形杆菌属(Proteus)；芽孢杆菌科(Bacillaceae)；根瘤菌科(Rhizobiceae)，例如根瘤菌属；螺菌科(Spirillaceae)，例如发光杆菌属(photobacterium)、发酵单胞菌属(Zymomonas)、沙雷氏菌属、气单胞菌属(Aeromonas)、弧菌属(Vibrio)、脱硫弧菌属(Desulfovibrio)、螺旋菌属(Spirillum)；乳杆菌科(Lactobacillaceae)；假单胞菌科(Pseudomonadaceae)，例如假单胞菌属和乙杆菌属；固氮菌科(Azotobacteraceae)和硝化杆菌科(Nitrobacteraceae)。真菌包括藻菌纲(Phycomycetes)和子囊菌纲(Ascomycetes)，例如酵母，如酵母属和裂殖酵母属(Schizosaccharomyces)；和担子菌纲(Basidiomycetes)酵母，例如红酵母属、短梗霉属、掷孢酵母属等等。
编码杀虫蛋白质的基因可以借助于电转化、PEG诱导的转化、热休克、转导、缀合等等引入。具体地，可以将编码杀虫蛋白质的基因克隆到穿梭载体例如pHT3101内(Lerecius等人(1989)FEMS Microbiol.Letts.60:211-218)。含有特定杀虫蛋白质基因的编码序列的穿梭载体pHT3101可以例如借助于电穿孔转化到根部定植的芽孢杆菌内(Lerecius等人(1989)FEMS Microbiol.Letts.60:211-218)。
可以设计表达系统，通过将适当的信号肽融合到杀虫蛋白质的氨基末端，使得杀虫蛋白质分泌到革兰氏阴性细菌的细胞质外。由大肠杆菌识别的信号肽包括OmpA蛋白(Ghrayeb等人(1984)EMBOJ，3:2437-2442)。
杀虫蛋白质及其活性变体可以在细菌宿主中发酵，并且可以将得到的细菌加工且以与苏云金芽孢杆菌菌株被用作杀虫喷雾的相同方式用作微生物喷雾。在杀虫蛋白质由芽孢杆菌分泌的情况下，使用本领域已知的操作去除或突变分泌信号。这种突变和/或缺失可防止杀虫蛋白质在发酵过程期间分泌到生长培养基内。杀虫蛋白质保留在细胞内，然后加工细胞以产生包封的杀虫蛋白质。
可替代地，通过将异源基因引入细胞宿主内来产生杀虫蛋白质。异源基因的表达直接或间接地导致杀虫剂的细胞内生产和维持。然后将这些细胞在细胞施用于靶害虫的环境时能延长细胞中产生的毒素的活性的条件下进行处理。所得到的产物保留了毒素的毒性。然后可以根据常规技术配制这些天然包封的杀虫蛋白质，用于施用于具有靶害虫的环境例如土壤、水和植物叶子。参见例如美国专利号6,468,523和美国公开号20050138685，以及其中引用的参考文献。在本发明中，经转化的微生物(其包括完整生物、细胞、孢子、杀虫蛋白质、杀虫组分、冲击害虫的组分、突变体、活细胞或死细胞和细胞组分，包括活细胞和死细胞以及细胞组分的混合物，并且包括破碎的细胞和细胞组分)或分离的杀虫蛋白质可以用可接受的载体配制成杀虫或农业组合物，其为例如悬浮液、溶液、乳剂、撒粉剂、可分散颗粒剂、可湿性粉剂和可乳化浓缩物、气溶胶、浸渍颗粒剂、佐剂、可涂布糊剂以及包封在例如聚合物物质中。
如本文公开的，农业组合物可以包含多肽、重组多肽或其变体或片段。本文公开的农业组合物可以应用于植物的环境或栽培区域，或者应用于植物、植物部分、植物细胞或种子。
上述公开的这种组合物可以通过添加表面活性剂、惰性载体、防腐剂、湿润剂、进食刺激剂、引诱剂、包封剂、粘合剂、乳化剂、染料、UV保护剂、缓冲剂、流动剂或肥料、微量营养素供体或影响植物生长的其他制剂而获得。一种或多种农业化学品，包括、但不限于除草剂、杀昆虫剂、杀真菌剂、杀细菌剂、杀线虫剂、杀软体动物剂、杀螨剂、植物生长调节剂、收获助剂和肥料，可以与载体、表面活性剂或配制领域常规采用的佐剂或其他组分相组合，以促进产品处理和对于特定靶害虫的应用。合适的载体和佐剂可以是固体或液体，并且对应于制剂技术中通常采用的物质，例如天然或再生矿物质、溶剂、分散剂、润湿剂、增粘剂、粘合剂或肥料。本发明的活性成分通常以组合物的形式应用，并且可以应用于待处理的作物区域、植物或种子。例如，本发明的组合物可以应用于准备在粮仓或筒仓等贮存或者在粮仓或筒仓等贮存期间的谷类。本发明的组合物可以与其他化合物同时或相继应用。应用本发明的活性成分或含有由本发明的细菌菌株产生的至少一种杀虫蛋白质的本发明农业化学组合物的方法包括但不限于叶面施用、种子包被和土壤施用。施用次数和施用速率取决于相应害虫的侵扰强度。
合适的表面活性试剂包括、但不限于阴离子化合物，例如金属的羧酸盐；长链脂肪酸的羧酸盐；N-酰基肌氨酸盐；磷酸与脂肪醇乙氧基化物的单酯或二酯或者这些酯的盐；脂肪醇硫酸盐例如十二烷基硫酸钠、十八烷基硫酸钠或十六烷基硫酸钠；乙氧基化脂肪醇硫酸盐；乙氧基化烷基酚硫酸盐；木质素磺酸盐；石油磺酸盐；烷基芳基磺酸盐例如烷基苯磺酸盐或低级烷基萘磺酸盐，例如丁基萘磺酸盐；磺化萘-甲醛缩合物的盐；磺化苯酚-甲醛缩合物的盐；更复杂的磺酸盐例如酰胺磺酸盐，例如油酸和N-甲基牛磺酸的磺化缩合产物；或二烷基磺基琥珀酸盐，例如琥珀酸二辛酯的磺酸钠。非离子型试剂包括脂肪酸酯、脂肪醇、脂肪酸酰胺或脂肪烷基或烯基取代的酚与环氧乙烷的缩合产物，多元醇醚的脂肪酸酯如脱水山梨糖醇脂肪酸酯，这些酯与环氧乙烷的缩合产物，例如聚氧乙烯脱水山梨糖醇脂肪酸酯，环氧乙烷和环氧丙烷的嵌段共聚物，炔二醇如2,4,7,9-四乙基-5-癸炔-4,7-二醇或乙氧基化炔二醇。阳离子表面活性试剂的实例包括例如脂肪族单胺、二胺或多胺如乙酸盐、萘酸盐或油酸盐；或含氧的胺如聚氧乙烯烷基胺的氧化胺；通过羧酸与二胺或多胺缩合制备的酰胺连接的胺；或季铵盐。
惰性材料的实例包括但不限于无机矿物质如高岭土、层状硅酸盐、碳酸盐、硫酸盐、磷酸盐，或植物材料如软木、粉状玉米芯、花生壳、稻壳和核桃壳。
本发明的组合物可以以直接施用的合适形式，或者作为主要组合物的浓缩物，其在施用前需要用合适量的水或其他稀释剂稀释。杀虫浓度将根据具体制剂的性质而变化，具体地，无论它是浓缩物还是直接使用。该组合物含有1至98％的固体或液体惰性载体，以及0至50％或0.1至50％的表面活性剂。这些组合物将以用于商业产物的标记速率施用，例如当以干燥形式时为约0.01lb-5.0lb/英亩，当以液体形式时为约0.01pts.-10pts./英亩。
在一个进一步的实施方案中，本文提供的组合物以及经转化的微生物和杀虫蛋白质可以在配制前进行处理，以在施用于靶害虫的环境时延长杀虫活性，只要预处理对该杀虫活性没有害处即可。这种处理可以通过化学和/或物理手段进行，只要处理不会有害地影响组合物的性质即可。化学试剂的例子包括但不限于卤化剂；醛如甲醛和戊二醛；抗感染药如苯扎氯铵；醇如异丙醇和乙醇；和组织固定剂如Bouin固定剂和Helly固定剂(参见例如Humason(1967)Animal Tissue Techniques(W.H.Freeman and Co.))。
在一个方面，可以通过将本发明的杀虫蛋白质施加到一个给定区域而杀死该区域内的害虫或减少其中的害虫数目。可替代地，杀虫蛋白质可以预防性地应用于环境区域，以预防易感害虫的侵扰。优选地，害虫摄取或接触杀虫有效量的所述多肽。“杀虫有效量”意指能够使至少一种害虫死亡或明显降低害虫生长、进食或正常生理发育的杀虫剂的量。这个量取决于这种因素，如例如待控制的特定靶害虫、特定环境、地点、植物、作物或待处理的农业地点、环境条件，以及杀虫有效多肽组合物的施用方法、速率、浓度、稳定性和数量而变化。制剂或组合物也可以根据气候条件、环境考虑因素和/或施用频率和/或害虫侵扰的严重程度而变化。
活性成分通常以组合物的形式应用，并且可以应用于待处理的作物区域、植物或种子。因此提供了用于对植物、植物细胞、种子、植物部分或栽培区域提供有效量的包含所述多肽、重组多肽或其活性变体或片段的农业组合物的方法。“有效量”是指足以杀死或控制害虫，或者导致害虫生长、进食或正常生理发育的显著下降的蛋白质或组合物的量。害虫数目、害虫生长、害虫进食或害虫正常发育的这种下降可以包括任何统计学上显著的下降，包括例如下降约5％、10％、15％、20％、25％、30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、85％、90％、95％或更多。例如，所述组合物可以应用于准备在粮仓或筒仓等中贮存或者在粮仓或筒仓等中贮存期间的谷类。所述组合物可以与其他化合物同时或相继应用。应用包含如本文公开的多肽、重组多肽或者其变体或片段中的至少一种的活性成分或农业化学组合物的方法包括但不限于叶面施用、种子包被和土壤施用。
提供了用于增加植物产量的方法。所述方法包括提供表达编码本文公开的杀虫多肽序列的多核苷酸的植物或植物细胞，以及使所述植物或其种子在侵染了所述多肽对其具有杀虫活性的害虫(或易受其侵染)的田地中生长。在一些实施方案中，所述多肽具有针对鳞翅目、鞘翅目、双翅目、半翅目或线虫害虫的杀虫活性、并且所述田地被鳞翅目、半翅目、鞘翅目、双翅目或线虫害虫侵扰。如本文定义的，植物的“产量”指由植物产生的生物质的质量和/或数量。“生物质”意指任何测量的植物产物。生物质产量中的增加是测量的植物产物的产量中的任何改善。增加植物产量具有几种商业应用。例如，增加植物叶生物量可以增加用于人或动物消费的叶用蔬菜的产量。另外，增加叶生物量可以用于增加植物衍生的药物或工业产物的生产。产量增加可以包括任何统计学上显著的增加，包括、但不限于与不表达所述杀虫序列的植物相比，产量有至少1％增加、至少3％增加、至少5％增加、至少10％增加、至少20％增加、至少30％、至少50％、至少70％、至少100％或更大增加。在具体方法中，由于表达本文公开的杀虫蛋白质的植物的害虫抗性改善，植物产量增加。杀虫蛋白质的表达导致害虫侵袭或进食的能力降低。
还可以用一种或多种化学组合物(包括一种或多种除草剂、杀昆虫剂或杀真菌剂)处理植物。
非限制性实施方案包括：
实施方案1.具有杀虫活性的分离的多肽，其包含
(a)包含选自由下述序列组成之组中的氨基酸序列的多肽：SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223或224；或
(b)包含与选自由下述序列组成之组中的氨基酸序列至少有表1所示的序列同一性百分比的氨基酸序列的多肽：SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223或224。
实施方案2.实施方案1的多肽，其中所述多肽包含SEQ ID No.1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223或224中所示的氨基酸序列。
实施方案3.实施方案2的多肽，其进一步包含异源氨基酸序列。
实施方案4.包含实施方案1、2、3中的至少一个所述的多肽的组合物。
实施方案5.编码实施方案1或2所述的多肽的重组核酸分子，其中所述重组核酸分子不是编码所述多肽的天然存在的序列。
实施方案6.实施方案5的重组核酸，其中所述核酸分子是已被设计用于在植物中表达的合成序列。
实施方案7.实施方案5或6的重组核酸分子，其中所述核酸分子与能够指导在植物细胞中的表达的启动子可操作地连接。
实施方案8.实施方案5或6的重组核酸分子，其中所述核酸分子与能够指导在细菌中的表达的启动子可操作地连接。
实施方案9.含有实施方案5-8中任何一个所述的重组核酸分子的宿主细胞。
实施方案10.实施方案9的宿主细胞，其中所述宿主细胞是细菌宿主细胞。
实施方案11.包含可操作地连接至重组核酸分子的启动子的DNA构建体，所述启动子驱动在植物细胞中的表达，所述重组核酸分子包含：
(a)编码包含下述任一氨基酸序列的多肽的核苷酸序列：SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223或224；或
(b)编码包含与选自下述序列的组中的氨基酸序列有至少表1中所示的序列同一性百分比的氨基酸序列的多肽的核苷酸序列：SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223或224。
实施方案12.实施方案11的DNA构建体，其中所述核苷酸序列是已被设计用于在植物中表达的合成DNA序列。
实施方案13.包含实施方案11的DNA构建体的载体。
实施方案14.含有实施方案11或12的DNA构建体或者实施方案13的载体的宿主细胞。
实施方案15.实施方案14的宿主细胞，其中所述宿主细胞是植物细胞。
实施方案16.包含实施方案14或15的宿主细胞的转基因植物。
实施方案17.包含实施方案9、10、14或15的宿主细胞的组合物。
实施方案18.实施方案17的组合物，其中所述组合物选自粉末、粉剂、丸剂、颗粒剂、喷雾剂、乳剂、胶体和溶液。
实施方案19.实施方案17的组合物，其中所述组合物包含按重量计约1％至约99％的所述多肽。
实施方案20.用于控制害虫群体的方法，其包括使所述群体与杀虫有效量的实施方案17-19中任何一个所述的组合物接触。
实施方案21.用于杀死害虫群体的方法，其包括使所述群体与杀虫有效量的实施方案17-19中任何一个所述的组合物接触。
实施方案22.用于产生具有杀虫活性的多肽的方法，其包括在其中编码所述多肽的核酸分子被表达的条件下培养实施方案9、10、14或15所述的宿主细胞。
实施方案23.具有稳定整合到其基因组内的DNA构建体的植物，所述DNA构建体包含编码具有杀虫活性的蛋白质的核苷酸序列，其中所述核苷酸序列包含：
(a)编码包含下述任一氨基酸序列的多肽的核苷酸序列：SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223或224；或
(b)编码包含与选自下述序列的组中的氨基酸序列有至少表1中所示的序列同一性百分比的氨基酸序列的多肽的核苷酸序列：SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223或224。
实施方案24.实施方案23所述的植物的转基因种子。
实施方案25.用于保护植物免受昆虫害虫的方法，其包括在植物或其细胞中表达编码杀虫多肽的核苷酸序列，其中所述核苷酸序列包含：
(a)编码包含下述任一氨基酸序列的多肽的核苷酸序列：SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223或224；或
(b)编码包含与选自下述序列的组中的氨基酸序列有至少表1中所示的序列同一性百分比的氨基酸序列的多肽的核苷酸序列：SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223或224。
实施方案26.实施方案25的方法，其中所述植物产生具有针对鳞翅目害虫或鞘翅目害虫中的至少一种的杀虫活性的杀虫多肽。
实施方案27.用于增加植物产量的方法，其包括在田间生长具有稳定整合到其基因组内的DNA构建体的植物或其种子，所述DNA构建体包含可操作地连接至编码杀虫多肽的核苷酸序列的启动子，所述启动子驱动在植物中的表达，其中所述核苷酸序列包含：
(a)编码包含下述任一氨基酸序列的多肽的核苷酸序列：SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223或224；或
(b)编码包含与选自下述序列的组中的氨基酸序列有至少表1中所示的序列同一性百分比的氨基酸序列的多肽的核苷酸序列：SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223或224。
实施方案28.提供了获得编码包含杀虫活性的改善多肽的多核苷酸的方法，其中所述改善多肽具有超过SEQ ID NO:1-224中任何一个的至少一种改善的性质，所述方法包括：
(a)将包含SEQ ID NO:1-224或者其活性变体或片段的多个亲本多核苷酸重组，以产生编码重组杀虫多肽的重组多核苷酸文库；
(b)筛选所述文库以鉴定编码改善的重组杀虫多肽的重组多核苷酸，所述重组多核苷酸相比于亲本多核苷酸具有改善的更好的性质；
(c)回收编码(b)中鉴定的改善的重组杀虫多肽的重组多核苷酸；和
(d)使用步骤(c)中回收的重组多核苷酸作为重复步骤(a)中的多个亲本多核苷酸之一，重复步骤(a)、(b)和(c)。
下述实施例以举例说明性而非限制性的方式提供。
实验
实验1：通过测序和DNA分析发现新型基因
微生物培养物在标准实验室培养基中以液体培养物生长。在DNA制备之前，使培养物生长至饱和(16至24小时)。通过去污剂裂解从细菌细胞中提取DNA，随后与二氧化硅基质结合并且用乙醇缓冲液洗涤。纯化的DNA用弱碱性水性缓冲液从二氧化硅基质中洗脱下来。
通过分光光度法测试用于测序的DNA的纯度和浓度。根据制造商的方案，使用Nextera XT文库制备试剂盒制备测序文库。根据Illumina HiSeq 2000系统用户指南协议，在HiSeq 2000上生成序列数据。
使用CLC Bio Assembly Cell软件包将测序读数装配成基因组草图。在组装之后，通过几种方法进行基因调用，并且分析所得到的基因序列，以鉴定杀虫基因的新型同系物。新型基因通过BLAST、结构域组成、以及与一组靶杀虫基因的成对比对进行鉴定。这些序列的概括在表1中阐述。
通过PCR从细菌DNA扩增同源性搜索中鉴定的基因，并且克隆到含有框内融合的纯化标签的细菌表达载体内。克隆的基因在大肠杆菌中表达并且通过柱层析纯化。在昆虫饮食生物测定研究中评价纯化的蛋白质，以鉴定活性蛋白质。
使用小麦胚芽和琼脂人造饮食执行昆虫饮食生物测定，将纯化蛋白质作为表面处理应用于所述饮食。将昆虫幼虫应用于经处理的饮食并且监测死亡率。
昆虫饮食生物测定使用包含在其中添加了纯化蛋白质的膜小袋中的蔗糖液体饮食执行。允许昆虫幼虫以饮食小袋为食并且监测死亡率。在生物测定中测试的昆虫包括褐臭蝽(BSB)Euschistus servus(褐臭蝽)和南绿蝽象(SGSB)Nezara viridula(稻绿蝽)。
实施例2.在大肠杆菌中的异源表达
将表3和表4中阐述的每个开放读码框克隆到含有麦芽糖结合蛋白(pMBP)的大肠杆菌表达载体内。将表达载体转化到BL21*RIPL内。用单菌落接种补充有羧苄青霉素的LB培养物，并且使用0.5％过夜培养物在37℃下生长过夜，接种新鲜培养物并且在37℃下培养至对数期。使用250mM IPTG在16℃下将培养物诱导18小时。将细胞形成团块并且重悬浮于补充有蛋白酶抑制剂的10mM Tris pH7.4和150mM NaCl中。通过SDS-PAGE评估蛋白质表达。
实施例3.针对鞘翅目和鳞翅目的杀虫活性
蛋白质表达：如实施例2中所述，将表3中阐述的每种序列在大肠杆菌中表达。接种400mL的LB并且生长至OD 600达到0.6。培养物在16℃下用0.25mM IPTG诱导过夜。将细胞离心下来，并且将细胞团块重悬浮于5mL缓冲液中。重悬浮物在冰上超声处理2分钟。
生物测定：从商业养虫室(Benzon Research Inc.，Carlisle，PA)购买秋粘虫(FAW)、玉米穗虫(CEW)、欧洲玉米螟(ECB)西南玉米螟(SWCB)和小菜蛾(DBM或Px)卵。将FAW、CEW、ECB和BCW卵孵化至羽化在测定设置的12小时内发生的点。将SWCB和DBM作为新生幼虫引入测定中。在含有多物种鳞翅目饮食(Southland Products Inc.，Lake Village，AR)的24孔盘中进行测定。将超声处理的裂解产物样品应用于饮食表面(饮食覆盖层)，并且允许蒸发且浸泡到饮食内。对于CEW、FAW、BCW、ECB和SWCB，将125μl超声处理的裂解产物加入饮食表面并干燥。对于DBM，将50μl 1:2稀释的超声处理的裂解产物加入饮食表面。将生物测定板用带针孔的平板密封膜密封。将板在26℃、65％相对湿度(RH)下在Percival中以16:8白天:黑夜循环温育5天。评价测定中的死亡率水平、生长抑制和进食抑制。
对于西方玉米根虫生物测定法，通过在24孔板(Cellstar，24孔，Greiner Bio)的孔中的饮食顶部表面上分配60μl体积并且允许干燥，在昆虫生物测定中评估蛋白质构建体/裂解产物。每孔含有500μl饮食(Marrone等人，1985)。使用精细尖端涂料刷在每个孔中引入十五至二十个新生幼虫，并且用膜(Viewseal，Greiner Bio One)覆盖板。将生物测定贮存于环境温度下，并且在第4天时对死亡率和/或生长/进食抑制进行评分。
对于科罗拉多马铃薯甲虫(CPB)，从马铃薯叶中切下8号木塞钻孔器大小的叶盘，并且浸入蛋白质构建物/裂解产物中直至彻底湿润，置于滤盘(Millipore，玻璃纤维滤纸，13mm)之上。将60μl dH2O加入每个滤盘中，并且置于24孔板(Cellstar，24孔，Greiner BioOne)的每个孔中。允许叶盘干燥，并且使用精细尖端涂料刷在每个孔中引入五至七个一龄幼虫。将板用膜(Viewseal，Greiner Bio One)覆盖，并且在膜的每个孔中穿孔。构建体用一式四份进行评估，并且在第3天时对死亡率和叶损害进行评分。
表3提供了各种序列针对鞘翅目和鳞翅目的杀虫活性的概括。表格代码：“-”指示并未看到活性；“+”指示杀虫活性；“NT”指示未经测试；“S”指示发育迟缓；“SS”指示轻微发育迟缓；“LF”指示低进食，“M”指示死亡率。
表3.针对鞘翅目和鳞翅目的杀虫活性概括。
实施例4.针对半翅目的杀虫活性
蛋白质表达：如实施例2中所述，将表4中阐述的每种序列在大肠杆菌中表达。接种400mL的LB并且生长至OD 600达到0.6。培养物在16℃下用0.25mM IPTG诱导过夜。将细胞离心下来，并且将细胞团块重悬浮于5mL缓冲液中。重悬浮物在冰上超声处理2分钟。
二龄SGSB从商业养虫室(Benzon Research Inc.，Carlisle，PA)获得。以超声处理的裂解产物样品与20％蔗糖的50％v/v比用于生物测定。将拉伸的石蜡膜用作进食膜，以使SGSB暴露于饮食/样品混合物。将板在25℃：21℃的16:8白天：黑夜循环于65％RH下温育5天。
对于每个样品评分死亡率。结果在表4中阐述。虚线指示未检测到死亡率。表4中列出的蛋白质显示针对南绿蝽象25％的死亡率或50％的死亡率(如所示)(4只中的1只臭虫死亡)。阴性对照(表达结合结构域的空载体和仅缓冲液)两者均未显示死亡率(4只中有0只臭虫死亡)。
表4.针对半翅目的杀虫活性概括
APG Seq ID 针对SGSB测试
APG00078 2 25％
实施例5.大豆的转化
将包含与在植物中有活性的启动子可操作连接的SEQ ID NO:1-224中的每一个或其活性变体或片段的DNA构建体克隆到转化载体内，并且引入土壤杆菌属内，如于2015年12月18日提交的PCT申请PCT/US2015/066702中所述，其通过引用整体并入本文。
接种前四天，将几个环的土壤杆菌划线接种到补充有合适抗生素**(壮观霉素、氯霉素和卡那霉素)的YEP*培养基的新鲜板上。细菌在28℃下在黑暗中生长两天。两天后，在125ml锥形瓶中将几个环的细菌转移到3ml具有抗生素的YEP液体培养基内。将烧瓶置于28℃下250RPM的旋转振荡器上过夜。接种前一天，将2-3ml过夜培养物转移至500ml锥形瓶中的具有抗生素的125ml YEP内。将烧瓶置于28℃下250RPM的旋转振荡器上过夜。
在接种之前，在OD 620处检查细菌培养物的OD。0.8-1.0的OD指示培养物处于对数期。培养物在Oakridge管中以4000RPM离心10分钟。弃去上清液，并且将团块重悬浮于大豆感染培养基(SI)体积中，以达到所需的OD。将培养物伴随定期混合保持直至需要接种。
接种前两天或三天，使用氯气将大豆种子表面灭菌。在通风橱中，将具有种子的培养皿置于盖打开的钟形罩中。将1.75ml 12N HCl缓慢加入在钟形罩内的250ml锥形瓶中的100ml漂白剂中。将盖立即放在钟形罩的顶部上。使种子灭菌14-16小时(过夜)。将顶部从钟形罩上取下，并且替换培养皿的盖子。然后将表面灭菌的培养皿在层流中打开约30分钟，以散开任何剩余的氯气。
将种子用无菌DI水或大豆感染培养基(SI)吸胀1-2天。将20-30粒种子在100x25mm培养皿中用液体覆盖，并且在24℃下在黑暗中温育。吸胀后，弃去不发芽的种子。
子叶外植体在具有无菌滤纸的无菌纸板上进行加工，其中无菌滤纸采用美国专利号7,473,822(通过引用并入本文)的方法使用SI培养基弄湿。
通常，每个处理接种16-20片子叶。弃去用于保持外植体的SI培养基，并且用25ml土壤杆菌属培养物(OD 620＝0.8-20)替换。在所有的外植体都浸没后，接种进行30分钟，伴随盘的周期旋转。30分钟后，去除土壤杆菌属培养物。
通过将一片无菌纸覆盖在大豆共培养基(SCC)上来制备共培养板。无需印迹，将接种的子叶近轴侧面朝下地在滤纸上培养。每块板上可以培养约20个外植体。将板用Parafilm密封，并且在24℃和约120μmoles m-2s-1(在Percival培养箱中)培养4-5天。
共培养后，将子叶在具有200mg/l头孢噻肟和特美汀的25ml大豆洗涤培养基中洗涤3次。将子叶印迹到无菌滤纸上，然后转移到大豆芽诱导培养基(SSI)。外植体的结节末端略微压入培养基内，其中远端以约45度保持高于表面。在每块板上培养不超过10个外植体。用Micropore带包裹板并且在24℃和约120μmoles m-2s-1在Percival中培养。
14天后将外植体转移到新鲜的SSI培养基中。弃去来自茎尖和子叶节的新芽。芽诱导在相同的条件下继续另外14天。
在芽诱导4周后，将子叶从结节端分离，并且在芽诱导区(芽垫)下面进行平行切割。将平行切割的区域放置在大豆芽伸长培养基(SSE)上，并且将外植体在24℃和约120μmoles m-2s-1下在Percival中培养。这个步骤每两周重复一次，高达8周，只要芽继续伸长。
当芽达到2-3cm的长度时，将它们转移到Plantcon容器中的大豆生根培养基(SR)中，并且在相同的条件下温育2周，或者直到根达到约3-4cm的长度。这之后，将植物转移到土壤。
注意，对于大豆转化提到的所有培养基都可见于Paz等人(2010)Agrobacterium-mediated transformation of soybean and recovery of transgenic soybean plants；Plant Transformation Facility of Iowa State University中，所述参考文献通过引用整体并入本文。(参见agron-www.agron.iastate.edu/ptf/protocol/Soybean.pdf.)
实施例6.玉蜀黍的转化
玉蜀黍穗最好在授粉后8-12天收集。将胚从穗中分离出来，尺寸为0.8-1.5mm的那些胚优选用于转化。将胚以盾片侧面向上的方式放置在合适的温育培养基如DN62A5S培养基(3.98g/L N6盐；1mL/L(1000X原液)N6维生素；800mg/L L-天冬酰胺；100mg/L肌醇；1.4g/L L-脯氨酸；100mg/L酪蛋白氨基酸；50g/L蔗糖；1mL/L(1mg/mL原液)2,4-D)上。然而，除DN62A5S外的培养基和盐是合适的，并且是本领域已知的。胚在黑暗中在25℃下温育过夜。然而，将胚温育过夜本身并不是必要的。
将所得到的外植体转移到网格正方形(30-40个/板)，转移到渗透培养基上约30-45分钟，然后转移到光束板(参见例如PCT公开号WO/0138514和美国专利号5,240,842)。设计用于在植物细胞中表达本发明的GRG蛋白质的DNA构建体使用气溶胶光束加速器加速到植物组织内，使用基本上如PCT公开号WO/0138514中所述的条件。光束照射后，将胚在渗透培养基上温育约30分钟，并且置于温育培养基上在黑暗中在25℃下过夜。为了避免过度损伤受光束照射的外植体，在转移至恢复培养基之前将它们温育至少24小时。然后将胚铺展到恢复期培养基上约5天，在25℃下在黑暗中，然后转移到选择培养基上。外植体在选择培养基中温育高达八周，这取决于所利用的特定选择的性质和特征。选择期后，将所得到的愈伤组织转移至胚成熟培养基，直至观察到成熟体细胞胚的形成。然后将得到的成熟体细胞胚置于弱光下，并且通过本领域已知的方法启动再生过程。使所得到的芽在生根培养基上生根，并且将所得到的植物转移到育苗盆中且作为转基因植物繁殖。
实施例7.针对线虫的杀虫活性
大豆孢囊线虫(Heterodera glycine)的体外测定
将大豆孢囊线虫分配到96孔测定板内，每孔具有100ul总体积和100个J2。如SEQID NO:1-224中任何一个所述的感兴趣蛋白质被分配到孔内，并且保持在室温下用于评价。最后，分析含有SCN J2的96孔板的运动性。数据表示为与对照相比的％抑制。命中被定义为大于或等于70％抑制。
大豆孢囊线虫(大豆孢囊线虫)的植物上测定
表达SEQ ID NO:1-224中的一个或多个的大豆植物如本文其他地方所述生成。3周龄的大豆切条用5000个SCN卵/植物进行接种。这种感染保持70天，然后收获用于计数已在植物上发育的SCN孢囊。数据表示为与对照相比的％抑制。命中被定义为大于或等于90％抑制。
南方根结线虫(Meloidogyne incognita)(根结线虫)体外测定
将根结线虫分配到96孔测定板内，每孔具有100ul总体积和100个J2。包含SEQ IDNO:1-398中任何一个的感兴趣蛋白质被分配到孔内，并且保持在室温下用于评价。最后，分析含有RKN J2的96孔板的运动性。数据表示为与对照相比的％抑制。命中被定义为大于或等于70％抑制。
南方根结线虫(根结线虫)植物上测定
表达SEQ ID NO:1-224中的一个或多个的大豆植物如本文其他地方所述生成。3周龄的大豆用5000个RKN卵/植物进行接种。这种感染保持70天，然后收获用于计数已在植物中发育的RKN卵。数据表示为与对照相比的％抑制。命中被定义为大于或等于90％抑制。
实施例8.关于杀虫活性的进一步测定
可以以多种方式测试SEQ ID NO:1-224中所示的各种多肽充当害虫的杀虫剂。一种这样的方法是执行进食测定。在这样的进食测定中，将害虫暴露于含有待测试的化合物的样品或对照样品。通常这通过将待测试的材料或这种材料的适当稀释物放置在害虫将摄取的材料例如人造饮食上执行。待测试的材料可以由液体、固体或浆料组成。待测试的材料可以放置在表面上，然后使其干燥。可替代地，待测试的材料可以与熔化的人造饮食混合，然后分配到测定室内。测定室可以是例如杯子、盘或微量滴定板的孔。
用于刺吸式害虫(例如蚜虫)的测定可能涉及通过分隔物(理想的是可被刺吸式昆虫的吸式口器刺穿的部分)使测试材料与昆虫隔开，以允许测试材料的摄取。通常将测试物质与进食刺激剂(如蔗糖)混合，以促进测试化合物的摄取。
其他类型的测定可以包括将测试物质显微注射到害虫的口腔或肠内，以及转基因植物的开发，随后测试害虫以转基因植物为食的能力。植物测试可能涉及到正常消耗的植物部分的分离，例如，附着到叶上的小笼，或者在含有昆虫的笼中分离整个植物。
测定害虫的其他方法和途径是本领域已知的，并且可见于例如Robertson和Preisler编辑(1992)Pesticide bioassays with arthropods，CRC，Boca Raton，Fla中。可替代地，测定通常在期刊Arthropod Management Tests和Journal of EconomicEntomology中或通过与美国昆虫学会(Entomological Society of America)(ESA)会员的讨论进行描述。可以表达SEQ ID NO:1-224中任何一个，并且用于如本文实施例3和4中所述的测定中。
说明书中提及的所有出版物和专利申请指示本发明所属领域的技术人员的技术水平。所有的出版物和专利申请都通过引用并入本文，其程度与每个个别出版物或专利申请被特别且个别地指出通过引用并入相同。
尽管为清楚理解起见，前述发明已通过举例说明和实施例的方式较为详细地进行了描述，但显而易见的是，可以在所附权利要求书的范围内实施某些改变和修改。
序列表
<110> AGBIOME, Inc.
<120> 杀虫基因和使用方法
<130> 9686991010353
<150> US 62/182,855
<151> 2015-06-22
<160> 224
<170> PatentIn version 3.5
<210> 1
<211> 724
<212> PRT
<213> 芽孢杆菌属的种(Bacillus sp.)
<400> 1
Leu Ser Ile Ile Arg Ile Arg Lys Met Asn Lys Lys Ile Ser Arg Gly
1 5 10 15
Arg Leu Cys Asn Met Glu Leu Lys Val Asn Asp Ile Glu Ile Ala Ser
20 25 30
Asn Asp Lys Gly Tyr Ser His Asp Ser Ser Ile Gly Tyr Ser Asn Pro
35 40 45
Asn Trp Met Asn Lys Met Pro Gly Thr Leu Lys Ile Ser Glu Leu Ser
50 55 60
Ile Pro Gly Thr His Asn Ser Met Ser Arg Tyr Gly Gly Gly Gly Leu
65 70 75 80
Tyr Pro Asp Asp Tyr Ile Lys Thr Gln Thr Met Asp Val Asn Thr Gln
85 90 95
Leu Asn Ser Gly Ile Arg Tyr Leu Asp Ile Arg Leu Arg Lys Glu Ser
100 105 110
Asn Ala Leu Leu Lys Ala Tyr His Gly Ser Val Asn Gln Asn Ala Ile
115 120 125
Phe Ser Ser Asp Ile Leu Asp Lys Ile Thr Ala Phe Leu Arg Thr Asn
130 135 140
Pro Gly Glu Thr Ile Phe Met Arg Val Lys Asn Glu Cys Asn Gly Pro
145 150 155 160
Gly Thr Gly Lys Cys Thr Asp Ala Ser Thr Thr Lys Thr Trp Ala Gln
165 170 175
Ile Phe Glu Asp Asn Tyr Tyr Asn Asn Ala Ser Tyr Ser Asn Tyr Phe
180 185 190
Trp Lys Gly Ala Ser Asn Asn Pro Thr Leu Asn Glu Val Lys Gly Lys
195 200 205
Ile Val Val Met Ser Asp Phe Pro Thr Asp Gly Lys Arg Phe Gly Ile
210 215 220
Pro Tyr Asn Thr Leu Asn Lys Gln Asp Gln Phe Asp Val Asp Asp Ser
225 230 235 240
Ala Asp Ala Met Tyr Ala Lys Trp Thr Ala Val Gly Asn His Leu Lys
245 250 255
Ala Ala Asn Ala Ser Asp Lys Gln Ser Ile Tyr Leu Asn Tyr Leu Ser
260 265 270
Gly Asn Gly Ala Trp Arg Phe Ile Ser Met Gly Ala Ala Pro Tyr Phe
275 280 285
Val Ala Ser Gly Tyr Arg Gly Ser Gly Thr Asn Glu Ser Met Lys Met
290 295 300
Ile Thr Glu His Arg Thr Asp Lys Trp Pro Asp Tyr Pro Arg Gly Tyr
305 310 315 320
Tyr Gly Gln Val Phe Tyr Gly Gly Thr Asn Leu Leu Thr Thr Gln Tyr
325 330 335
Ile Gln Gln Trp Pro Ile Arg His Thr Gly Ile Ile Ala Ala Asp Phe
340 345 350
Pro Gly Lys Gly Leu Ile Asp Arg Val Ile Arg Leu Asn Glu Arg His
355 360 365
Asn Ser Gly Asp Val Arg Tyr Ile Thr Pro Lys Gly Ser Ser Gly Leu
370 375 380
Arg Val Gly Phe Gly Gly Asp Ala Tyr Leu Arg Asn Ser Tyr Val Val
385 390 395 400
Tyr Arg Asn Gly Thr Tyr Ile Gly His Val Asp Ser Gly Ser Pro Tyr
405 410 415
Tyr Ser Tyr Trp Asp Lys Thr Asp Val Gly His Asp Leu Arg Phe Glu
420 425 430
Gly Leu Ala Leu Phe Thr Gly Asp Lys Ile Asp Val Tyr Val Lys Asn
435 440 445
Gly Gly Ser Ser Thr Leu Ile Lys Ser Gln Thr Leu Thr Val Asp Glu
450 455 460
Gln Glu Gly Glu Glu Val Arg Ile Pro Asp Gly Lys Tyr Ser Ile Val
465 470 475 480
Ser Ala Leu Asn Asn Ser Ser Glu Val Ala Met Gln Thr Ser Leu Ser
485 490 495
Asn Asn Arg Tyr Asn Val Val Leu Trp Gly His Thr Asn Arg Glu Leu
500 505 510
Asn Gly Gln Trp Asp Leu Val Tyr Asp Ser Asn Lys Lys Ala Tyr Gln
515 520 525
Leu Lys Ser Ala Trp Lys Val Asp Glu Ala Leu Thr Trp Thr Gln Asp
530 535 540
Ser Ile Asn Val Tyr Thr Ser Pro Ser Met Gly Thr Tyr Asp Gly Ala
545 550 555 560
Tyr Trp Ile Leu Lys Arg Ala Gly Asn Gly Tyr Leu Tyr Leu Glu Asn
565 570 575
Lys Ala Ser Lys Thr Val Leu Asp Val Thr Gly Gly Gly Thr Ala Asn
580 585 590
Gly Thr Asn Ile Asn Val Trp Gly Gln Val Gln Gly Ala Gln Asn Gln
595 600 605
Lys Phe Lys Leu Ile Gln Arg Thr Glu Lys Leu Glu Ala His Ile Asp
610 615 620
Ser Leu Tyr Arg Pro Gln Pro Gly Gln Lys Asn Arg Ser Ser Gly Asn
625 630 635 640
Phe Ser Leu Gly His Leu Ala Asp Gly Thr Arg Val Gln Val Ser Ile
645 650 655
Glu Gly Pro Gly Ala Ser Val Leu Asn Phe Arg Val Met Arg Asp Lys
660 665 670
Ser Gly Asp Thr Asp Pro Thr Ile Trp Ser Asn Val Lys His Gly Ser
675 680 685
Ile Val Thr Ile Pro Ser Gly Ala Asn Lys Asn Asn Leu Tyr Ile Ala
690 695 700
Asn Pro Gly Gly Tyr Ser Gly Asn Gly Thr Phe Lys Ala Lys Phe Gln
705 710 715 720
Thr Leu Gln Asn
<210> 2
<211> 704
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 2
Met Glu Leu Lys Val Asn Asp Ile Glu Ile Ala Ser Asn Asp Lys Gly
1 5 10 15
Tyr Ser His Asp Ser Ser Ile Gly Tyr Ser Asn Pro Asn Trp Met Asn
20 25 30
Lys Met Pro Gly Thr Leu Lys Ile Ser Glu Leu Ser Ile Pro Gly Thr
35 40 45
His Asn Ser Met Ser Arg Tyr Gly Gly Gly Gly Leu Tyr Pro Asp Asp
50 55 60
Tyr Ile Lys Thr Gln Thr Met Asp Val Asn Thr Gln Leu Asn Ser Gly
65 70 75 80
Ile Arg Tyr Leu Asp Ile Arg Leu Arg Lys Glu Ser Asn Ala Leu Leu
85 90 95
Lys Ala Tyr His Gly Ser Val Asn Gln Asn Ala Ile Phe Ser Ser Asp
100 105 110
Ile Leu Asp Lys Ile Thr Ala Phe Leu Arg Thr Asn Pro Gly Glu Thr
115 120 125
Ile Phe Met Arg Val Lys Asn Glu Cys Asn Gly Pro Gly Thr Gly Lys
130 135 140
Cys Thr Asp Ala Ser Thr Thr Lys Thr Trp Ala Gln Ile Phe Glu Asp
145 150 155 160
Asn Tyr Tyr Asn Asn Ala Ser Tyr Ser Asn Tyr Phe Trp Lys Gly Ala
165 170 175
Ser Asn Asn Pro Thr Leu Asn Glu Val Lys Gly Lys Ile Val Val Met
180 185 190
Ser Asp Phe Pro Thr Asp Gly Lys Arg Phe Gly Ile Pro Tyr Asn Thr
195 200 205
Leu Asn Lys Gln Asp Gln Phe Asp Val Asp Asp Ser Ala Asp Ala Met
210 215 220
Tyr Ala Lys Trp Thr Ala Val Gly Asn His Leu Lys Ala Ala Asn Ala
225 230 235 240
Ser Asp Lys Gln Ser Ile Tyr Leu Asn Tyr Leu Ser Gly Asn Gly Ala
245 250 255
Trp Arg Phe Ile Ser Met Gly Ala Ala Pro Tyr Phe Val Ala Ser Gly
260 265 270
Tyr Arg Gly Ser Gly Thr Asn Glu Ser Met Lys Met Ile Thr Glu His
275 280 285
Arg Thr Asp Lys Trp Pro Asp Tyr Pro Arg Gly Tyr Tyr Gly Gln Val
290 295 300
Phe Tyr Gly Gly Thr Asn Leu Leu Thr Thr Gln Tyr Ile Gln Gln Trp
305 310 315 320
Pro Ile Arg His Thr Gly Ile Ile Ala Ala Asp Phe Pro Gly Lys Gly
325 330 335
Leu Ile Asp Arg Val Ile Arg Leu Asn Glu Arg His Asn Ser Gly Asp
340 345 350
Val Arg Tyr Ile Thr Pro Lys Gly Ser Ser Gly Leu Arg Val Gly Phe
355 360 365
Gly Gly Asp Ala Tyr Leu Arg Asn Ser Tyr Val Val Tyr Arg Asn Gly
370 375 380
Thr Tyr Ile Gly His Val Asp Ser Gly Ser Pro Tyr Tyr Ser Tyr Trp
385 390 395 400
Asp Lys Thr Asp Val Gly His Asp Leu Arg Phe Glu Gly Leu Ala Leu
405 410 415
Phe Thr Gly Asp Lys Ile Asp Val Tyr Val Lys Asn Gly Gly Ser Ser
420 425 430
Thr Leu Ile Lys Ser Gln Thr Leu Thr Val Asp Glu Gln Glu Gly Glu
435 440 445
Glu Val Arg Ile Pro Asp Gly Lys Tyr Ser Ile Val Ser Ala Leu Asn
450 455 460
Asn Ser Ser Glu Val Ala Met Gln Thr Ser Leu Ser Asn Asn Arg Tyr
465 470 475 480
Asn Val Val Leu Trp Gly His Thr Asn Arg Glu Leu Asn Gly Gln Trp
485 490 495
Asp Leu Val Tyr Asp Ser Asn Lys Lys Ala Tyr Gln Leu Lys Ser Ala
500 505 510
Trp Lys Val Asp Glu Ala Leu Thr Trp Thr Gln Asp Ser Ile Asn Val
515 520 525
Tyr Thr Ser Pro Ser Met Gly Thr Tyr Asp Gly Ala Tyr Trp Ile Leu
530 535 540
Lys Arg Ala Gly Asn Gly Tyr Leu Tyr Leu Glu Asn Lys Ala Ser Lys
545 550 555 560
Thr Val Leu Asp Val Thr Gly Gly Gly Thr Ala Asn Gly Thr Asn Ile
565 570 575
Asn Val Trp Gly Gln Val Gln Gly Ala Gln Asn Gln Lys Phe Lys Leu
580 585 590
Ile Gln Arg Thr Glu Lys Leu Glu Ala His Ile Asp Ser Leu Tyr Arg
595 600 605
Pro Gln Pro Gly Gln Lys Asn Arg Ser Ser Gly Asn Phe Ser Leu Gly
610 615 620
His Leu Ala Asp Gly Thr Arg Val Gln Val Ser Ile Glu Gly Pro Gly
625 630 635 640
Ala Ser Val Leu Asn Phe Arg Val Met Arg Asp Lys Ser Gly Asp Thr
645 650 655
Asp Pro Thr Ile Trp Ser Asn Val Lys His Gly Ser Ile Val Thr Ile
660 665 670
Pro Ser Gly Ala Asn Lys Asn Asn Leu Tyr Ile Ala Asn Pro Gly Gly
675 680 685
Tyr Ser Gly Asn Gly Thr Phe Lys Ala Lys Phe Gln Thr Leu Gln Asn
690 695 700
<210> 3
<211> 401
<212> PRT
<213> 芽孢杆菌属的种
<400> 3
Met Arg Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ile Ser Glu Ala Ala Ala Pro Glu
20 25 30
Asn Asn Arg Tyr Tyr Asn Ile Gln Leu Lys Ser Asn Pro Lys Lys Val
35 40 45
Trp Asn Val Ser Gln Ala Ser Asn Asp Asn Asp Arg Ala Ile Leu Leu
50 55 60
Trp Gln Gly Gly Ser Ala Asp His Glu Lys Phe Ala Phe Phe Gln Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Val Val
85 90 95
Thr Phe Gly Thr Gly Ser Trp Ile Ser Gly Gly Pro Ile Pro Ala Ser
100 105 110
Glu Thr Leu Gln Gln Gln Ser Trp Thr Gly Glu Ala Lys Gln Lys Trp
115 120 125
Asn Leu Arg Asp Leu Gly Ser Asn Gly Asp Arg Ser Asn Asn Tyr Glu
130 135 140
Ile Val Asn Gln Ala Asn Gly Lys Val Pro Ser Tyr Arg Trp Ile Gly
145 150 155 160
Ser Thr Val Asp Asn Thr Glu Phe Leu Gln Leu Asp Asn Ser Asn Pro
165 170 175
Ser Asp Ala Asp Lys Val Phe Thr Leu Phe Asn Gly Thr Gly Asn Leu
180 185 190
Phe Gly Ile Asp Ile Thr Phe Phe Asn Asp Thr Ile Ser Val Pro Lys
195 200 205
Leu Pro Ala Ile Gly Thr Arg Pro Asp Val Pro Asp Tyr Thr Gly Gly
210 215 220
Val Asn Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala
225 230 235 240
Ser Leu Ile Pro Tyr Phe Met Val Lys Asp Asn Gln Ala Asn Asp Tyr
245 250 255
Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr
260 265 270
Trp Asp Lys Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser Glu Ala
275 280 285
Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys Met Thr
290 295 300
Asp Thr Leu Ser Met Asn Phe Gly Ala Asp Leu Gly Ile Lys Phe Gly
305 310 315 320
Asp Ala Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile Gln Thr
325 330 335
Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg Ser Tyr
340 345 350
Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln Tyr Gln
355 360 365
Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val Ser
370 375 380
Asp Pro Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg Lys Thr
385 390 395 400
Ser
<210> 4
<211> 1235
<212> PRT
<213> 芽孢杆菌属的种
<400> 4
Met Lys Gln Gln Tyr Asn Asn Glu Tyr Glu Ile Ile Asp Asn Asp Val
1 5 10 15
Met Gly Tyr Gln Pro Arg Phe Pro Leu Ala Gln Ala Pro Gly Ala Glu
20 25 30
Leu Gln Gln Met Asn Tyr Lys Asp Trp Met Asn Asn Met Cys Ala Glu
35 40 45
Gly Glu Ser Arg Glu Thr Phe Ala Asp Ile Thr Val Lys Asp Gly Val
50 55 60
Thr Val Ala Ile Ser Ile Val Ala Ala Val Leu Gly Val Ser Phe Pro
65 70 75 80
Val Ser Ala Ala Gly Val Ser Ile Ile Ser Thr Leu Ile Pro Tyr Trp
85 90 95
Trp Pro Glu Thr Ala Gly Thr Pro Gly Thr Ser Gln Ala Gln Val Thr
100 105 110
Trp Glu Arg Leu Met Ser Ala Ala Glu Gln Leu Ser Asn Gln Arg Ile
115 120 125
Leu Glu Ser Lys Arg Ser Asp Ala Ile Ala Arg Trp Gln Gly Ile Gln
130 135 140
Ser Leu Gly Arg Asp Phe Tyr Gln Ala Ile Cys Asp Leu Arg Glu Asp
145 150 155 160
Pro Asp Asn Glu Tyr Lys Lys Thr Lys Val Arg Asn Thr Phe Asp Ser
165 170 175
Val Glu Asp Lys Leu Lys Thr Ser Met Pro Phe Phe Arg Ala Gln Lys
180 185 190
Phe Glu Ile Pro Met Leu Ser Met Tyr Ala Gln Ala Ala Asn Met His
195 200 205
Leu Leu Leu Leu Arg Glu Val Val Gln Asn Gly Val Ser Trp Gly Phe
210 215 220
Gln Arg Tyr Glu Val Asp Arg Tyr Tyr Ser His Thr Asp Pro Phe Leu
225 230 235 240
Gly Asn Pro Gly Leu Leu Gln Leu Leu Glu Glu Tyr Thr Asp Tyr Cys
245 250 255
Val Gln Trp Tyr Asn Thr Gly Leu His Gln Gln Tyr Glu Asn Asn Arg
260 265 270
Tyr Asn Trp Asp Thr Phe Asn Asp Phe Arg Arg Asp Met Thr Thr Met
275 280 285
Val Leu Asp Ile Val Ser Leu Trp Pro Thr Tyr Asp Pro Lys Arg Tyr
290 295 300
Pro Leu Pro Thr Lys Ser Gln Leu Thr Arg Thr Val Tyr Thr Asp Leu
305 310 315 320
Leu Gly Phe Ser Gly Asp Ile Glu Tyr Leu Gln Ile Asp Ile Glu Arg
325 330 335
Ala Glu Glu Glu Leu Val Gln Arg Leu Gly Leu Phe Thr Trp Pro Arg
340 345 350
Glu Leu Ile Phe Gln Leu Lys Ser Leu Asn Gln Ile Asn Phe Val Thr
355 360 365
Gly Ser Glu Met Thr Phe Asn Tyr Thr Ala Ser Ala Asn Gly Tyr Arg
370 375 380
Glu Arg Lys Gly Asp Pro Gly Glu Thr Arg Glu Thr Val Val Ile Pro
385 390 395 400
Ser Pro Asp Val Gly Asp Asp Ile Trp Arg Ile Ser Thr Gln Val Asn
405 410 415
Thr Thr Gln Ile Pro Asn Ala Asn Phe Val Arg Gly Trp Asn Phe Ser
420 425 430
Phe Thr Gln Ser Leu Asp Gln Lys Ile Ala Trp Arg Thr Thr Ser Ser
435 440 445
Pro Glu Ile Val Met Gln Gly Leu Ser Cys His Gly Pro Ser Ile Gly
450 455 460
Ser Cys Asp Leu Cys Ser Ser Asn Asn Ser Cys Arg Ser Ile Asn Pro
465 470 475 480
Asn Tyr Ser Ser Pro Cys Asp Asp Lys Ser Val Tyr Ser His Arg Phe
485 490 495
Ser Tyr Leu Gly Ala Gly Leu Lys Ser Asp Leu Thr Arg Leu Thr Tyr
500 505 510
Phe Ser Tyr Gly Trp Thr His Val Ser Ala Asp Lys Asn Asn Leu Leu
515 520 525
Asp Thr His Met Ile Thr Gln Ile Pro Ala Val Lys Gly Tyr Asn Leu
530 535 540
Tyr Gln Gly Ala Arg Val Ile Lys Gly Pro Gly Ser Thr Gly Gly Asp
545 550 555 560
Leu Val Glu Leu Gly Val Asn Gly Gly Val Phe Val Lys Val Val Thr
565 570 575
Pro Ala Ser Glu Thr Val Ser Gly Tyr Thr Ile Arg Leu Arg Tyr Ala
580 585 590
Cys Lys Thr Glu Ile Leu Leu Phe Phe Arg Ile Arg Gln Pro Asp Ser
595 600 605
Asp Asp Arg Val Thr Ile Val Asn Leu Pro Ala Thr Thr Ser Asp Asp
610 615 620
Asn Leu Thr Tyr Ile Ala Phe Asn Tyr Tyr Asn Ser Trp Leu Gly Leu
625 630 635 640
Glu Asn Val Arg Ala Thr Gln Glu Glu Ser Ile Ile Phe Leu Gln Asn
645 650 655
Phe Ser Asn Glu Asn Thr Ile Ile Ile Asp Lys Ile Glu Phe Ile Pro
660 665 670
Ile Glu Gly Ser Leu Glu Val Tyr Gln Ser Asn Gln Asp Leu Glu Lys
675 680 685
Ala Arg Lys Ala Val Asn Ala Leu Phe Thr Asn Asp Thr Lys Asn Ala
690 695 700
Leu Gln Leu Asp Val Thr Asp Tyr Ala Val Asp Gln Ala Ala Asn Leu
705 710 715 720
Val Ala Cys Val Ser Asp Glu Phe His Gly Gln Glu Lys Met Ile Leu
725 730 735
Leu Asp Gln Val Lys Phe Ala Lys Arg Leu Ser Gln Thr Arg Asn Leu
740 745 750
Leu Asn His Gly Asp Phe Glu Ser Pro Asp Trp Ser Lys Glu Asn Gly
755 760 765
Trp Arg Thr Ser His His Val Ser Val Arg Ser Asp Asn Pro Ile Phe
770 775 780
Lys Gly Arg Tyr Leu His Met Pro Gly Ala Thr Ser Pro Gln Phe Ser
785 790 795 800
Asn Asn Ile Tyr Leu Thr Tyr Val Tyr Gln Lys Val Asp Glu Ser Lys
805 810 815
Leu Arg Ser Tyr Thr Arg Tyr Leu Val Arg Gly Phe Val Gly Asn Ser
820 825 830
Lys Asp Leu Glu Leu Leu Val Glu Arg Tyr Gly Lys Asp Val His Val
835 840 845
Glu Met Asp Val Pro Ser Asp Ile Gln Tyr Thr Leu Pro Met Asn Glu
850 855 860
Trp Gly Gly Phe Asp Arg Cys Lys Pro Ala Ser Tyr Gln Ala Arg Ser
865 870 875 880
Pro His Thr Cys Thr Cys Gln Asp Thr Ala Val Ala His Thr Asp Cys
885 890 895
Gln Cys Lys Asp Thr Val Asn Arg Thr Ser Thr Asn Met Tyr Thr Asn
900 905 910
Val Pro Thr Gly Ser Ala Gly Tyr Arg Asn Glu Ser His Asp His Lys
915 920 925
Thr Cys Gly Cys Lys Asp Pro His Val Phe Thr Tyr His Ile Asp Thr
930 935 940
Gly Tyr Val Asp Gln Glu Glu Asn Val Gly Leu Phe Phe Ala Leu Lys
945 950 955 960
Ile Ala Ser Glu Asn Gly Val Ala Asn Ile Asp Asn Leu Glu Ile Ile
965 970 975
Glu Ala Gln Pro Leu Thr Gly Glu Ala Leu Ala Arg Val Lys Asn Arg
980 985 990
Glu Gln Lys Trp Lys Gln Glu Met Ala Lys Lys Arg Leu Glu Thr Glu
995 1000 1005
Arg Ala Val Gln Ala Ala Gln Gly Ala Ile Gln Pro Leu Phe Thr
1010 1015 1020
Asn Gly Gln Tyr Asn Arg Leu Gln Phe Glu Thr Leu Phe Pro Gln
1025 1030 1035
Ile Val His Ala Glu Arg Leu Val Gln Gln Ile Pro Tyr Val His
1040 1045 1050
His Pro Phe Leu Asn Gly Ala Leu Leu Ala Val Pro Asp Met Asn
1055 1060 1065
Phe Glu Ile Val Gln His Leu Leu Thr Val Ile Gly Asn Ala Arg
1070 1075 1080
Ala Leu Tyr Glu Gly Arg Asn Leu Val Arg Asn Gly Thr Phe Ser
1085 1090 1095
Ser Gly Thr Glu Ser Trp His Val Ser Glu Gly Val Lys Val Lys
1100 1105 1110
Pro Leu Gln Asn Thr Ser Val Leu Val Leu Ser Glu Trp Asn His
1115 1120 1125
Glu Ala Ser Gln Gln Leu Arg Ile Asp Pro Asp Arg Gly Tyr Val
1130 1135 1140
Leu Arg Val Thr Ala Arg Lys Glu Gly Ala Gly Lys Gly Thr Val
1145 1150 1155
Thr Met Ser Asp Cys Ala Asp Tyr Thr Glu Ile Leu Thr Phe Thr
1160 1165 1170
Ser Cys Asp Tyr Asn Thr Ile Gly Thr Gln Thr Met Thr Gly Gly
1175 1180 1185
Thr Leu Ser Gly Phe Val Thr Lys Thr Leu Glu Ile Phe Pro Asp
1190 1195 1200
Thr Asp Arg Ile Arg Ile Asp Ile Gly Glu Thr Glu Gly Thr Phe
1205 1210 1215
Lys Ile Glu Ser Val Glu Leu Ile Cys Met Glu Gln Met Asp Asp
1220 1225 1230
His Leu
1235
<210> 5
<211> 673
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 5
Met Lys Gln Gln Tyr Asn Asn Glu Tyr Glu Ile Ile Asp Asn Asp Val
1 5 10 15
Met Gly Tyr Gln Pro Arg Phe Pro Leu Ala Gln Ala Pro Gly Ala Glu
20 25 30
Leu Gln Gln Met Asn Tyr Lys Asp Trp Met Asn Asn Met Cys Ala Glu
35 40 45
Gly Glu Ser Arg Glu Thr Phe Ala Asp Ile Thr Val Lys Asp Gly Val
50 55 60
Thr Val Ala Ile Ser Ile Val Ala Ala Val Leu Gly Val Ser Phe Pro
65 70 75 80
Val Ser Ala Ala Gly Val Ser Ile Ile Ser Thr Leu Ile Pro Tyr Trp
85 90 95
Trp Pro Glu Thr Ala Gly Thr Pro Gly Thr Ser Gln Ala Gln Val Thr
100 105 110
Trp Glu Arg Leu Met Ser Ala Ala Glu Gln Leu Ser Asn Gln Arg Ile
115 120 125
Leu Glu Ser Lys Arg Ser Asp Ala Ile Ala Arg Trp Gln Gly Ile Gln
130 135 140
Ser Leu Gly Arg Asp Phe Tyr Gln Ala Ile Cys Asp Leu Arg Glu Asp
145 150 155 160
Pro Asp Asn Glu Tyr Lys Lys Thr Lys Val Arg Asn Thr Phe Asp Ser
165 170 175
Val Glu Asp Lys Leu Lys Thr Ser Met Pro Phe Phe Arg Ala Gln Lys
180 185 190
Phe Glu Ile Pro Met Leu Ser Met Tyr Ala Gln Ala Ala Asn Met His
195 200 205
Leu Leu Leu Leu Arg Glu Val Val Gln Asn Gly Val Ser Trp Gly Phe
210 215 220
Gln Arg Tyr Glu Val Asp Arg Tyr Tyr Ser His Thr Asp Pro Phe Leu
225 230 235 240
Gly Asn Pro Gly Leu Leu Gln Leu Leu Glu Glu Tyr Thr Asp Tyr Cys
245 250 255
Val Gln Trp Tyr Asn Thr Gly Leu His Gln Gln Tyr Glu Asn Asn Arg
260 265 270
Tyr Asn Trp Asp Thr Phe Asn Asp Phe Arg Arg Asp Met Thr Thr Met
275 280 285
Val Leu Asp Ile Val Ser Leu Trp Pro Thr Tyr Asp Pro Lys Arg Tyr
290 295 300
Pro Leu Pro Thr Lys Ser Gln Leu Thr Arg Thr Val Tyr Thr Asp Leu
305 310 315 320
Leu Gly Phe Ser Gly Asp Ile Glu Tyr Leu Gln Ile Asp Ile Glu Arg
325 330 335
Ala Glu Glu Glu Leu Val Gln Arg Leu Gly Leu Phe Thr Trp Pro Arg
340 345 350
Glu Leu Ile Phe Gln Leu Lys Ser Leu Asn Gln Ile Asn Phe Val Thr
355 360 365
Gly Ser Glu Met Thr Phe Asn Tyr Thr Ala Ser Ala Asn Gly Tyr Arg
370 375 380
Glu Arg Lys Gly Asp Pro Gly Glu Thr Arg Glu Thr Val Val Ile Pro
385 390 395 400
Ser Pro Asp Val Gly Asp Asp Ile Trp Arg Ile Ser Thr Gln Val Asn
405 410 415
Thr Thr Gln Ile Pro Asn Ala Asn Phe Val Arg Gly Trp Asn Phe Ser
420 425 430
Phe Thr Gln Ser Leu Asp Gln Lys Ile Ala Trp Arg Thr Thr Ser Ser
435 440 445
Pro Glu Ile Val Met Gln Gly Leu Ser Cys His Gly Pro Ser Ile Gly
450 455 460
Ser Cys Asp Leu Cys Ser Ser Asn Asn Ser Cys Arg Ser Ile Asn Pro
465 470 475 480
Asn Tyr Ser Ser Pro Cys Asp Asp Lys Ser Val Tyr Ser His Arg Phe
485 490 495
Ser Tyr Leu Gly Ala Gly Leu Lys Ser Asp Leu Thr Arg Leu Thr Tyr
500 505 510
Phe Ser Tyr Gly Trp Thr His Val Ser Ala Asp Lys Asn Asn Leu Leu
515 520 525
Asp Thr His Met Ile Thr Gln Ile Pro Ala Val Lys Gly Tyr Asn Leu
530 535 540
Tyr Gln Gly Ala Arg Val Ile Lys Gly Pro Gly Ser Thr Gly Gly Asp
545 550 555 560
Leu Val Glu Leu Gly Val Asn Gly Gly Val Phe Val Lys Val Val Thr
565 570 575
Pro Ala Ser Glu Thr Val Ser Gly Tyr Thr Ile Arg Leu Arg Tyr Ala
580 585 590
Cys Lys Thr Glu Ile Leu Leu Phe Phe Arg Ile Arg Gln Pro Asp Ser
595 600 605
Asp Asp Arg Val Thr Ile Val Asn Leu Pro Ala Thr Thr Ser Asp Asp
610 615 620
Asn Leu Thr Tyr Ile Ala Phe Asn Tyr Tyr Asn Ser Trp Leu Gly Leu
625 630 635 640
Glu Asn Val Arg Ala Thr Gln Glu Glu Ser Ile Ile Phe Leu Gln Asn
645 650 655
Phe Ser Asn Glu Asn Thr Ile Ile Ile Asp Lys Ile Glu Phe Ile Pro
660 665 670
Ile
<210> 6
<211> 404
<212> PRT
<213> 芽孢杆菌属的种
<400> 6
Met Arg Lys Tyr Lys Lys Leu Ala Val Val Val Pro Leu Ala Gly Met
1 5 10 15
Leu Met Ser Gly Ala Gly Val Leu Gly Gly Ala Thr Ser Ala His Ala
20 25 30
Asp Arg Ile Asn Ile Asp Gly Val Arg Thr Gly Gly Pro Val Ser Asp
35 40 45
Gly Gly Asn Phe Ser Asp Thr Ser Gly Gly Gln Thr Phe Ser Asp Lys
50 55 60
Glu Leu Ala Asn Ile Tyr Leu Gln His Trp Lys Glu Gly Lys Tyr Gly
65 70 75 80
Asn Trp Arg Asp Gly Leu Tyr Met Ser His Gly Asp Phe Ser Asp Phe
85 90 95
Pro Pro Glu Phe Lys Ala Gly Val Met Ser Pro Gly Tyr Leu Ile Pro
100 105 110
Gln Ala Gln Leu Pro Ala Ile Ile Glu Lys Ile Lys Asn Thr Glu Gln
115 120 125
Leu Glu Leu Met Asp Leu Leu Gln Ile Leu Thr Pro Glu Thr Thr Val
130 135 140
Asp Lys Asp Val Gln Lys His Arg Tyr Leu Thr Val Asn Thr Asp Lys
145 150 155 160
Lys Thr Arg Ile Asn Ile Asp Lys Thr Glu Val Ile Ser Ala Pro Ile
165 170 175
Ala Ala Pro Gln Lys Val Thr Asn Asp Thr Asp Gln Glu Gly Ser Ala
180 185 190
Thr Ala Pro Ser Ile Ser Lys Lys Phe Thr Ser Thr Ile Ser Arg Thr
195 200 205
Asp Thr His Ser Thr Thr Leu Gly Met Lys Gln Thr Ile Lys Gly Gly
210 215 220
Ile Asp Phe Leu Gly Ile Ala Glu Gly Asp Phe Ser Gln Glu Phe Asn
225 230 235 240
Glu Ser Tyr Thr Tyr Ser Asn Ala Ser Gln Lys Leu Thr Ser Asp Glu
245 250 255
Thr Thr Ile Thr Ser Gly Thr Val Thr Ile Lys Thr Gln Pro His Ser
260 265 270
Ala Tyr Lys Phe Asp Ile Ile Phe Gln Gln Val Lys Asn Ser Gly Thr
275 280 285
Val Thr Gly Thr Ser His Leu Gly Gly Gly Tyr Gly Ser Ser Gly Lys
290 295 300
Asp Ala Tyr Tyr Asp Leu Ser Ile Tyr Lys Lys Met Lys Val Ile Gln
305 310 315 320
Asp Leu Tyr Pro Gln Leu Trp Ala Val Leu Gln Ala Lys Gly Ile Asp
325 330 335
Val Asn Asp Thr Thr Gln Glu Val Asn Tyr Thr Gly Ala Ile Ser Phe
340 345 350
Glu Gly Ile Thr Gly Ala Lys Ile Asp Val Thr Val Lys Asn Leu Ala
355 360 365
Thr Gly Asp Val Ile Asn Arg Leu Ile Thr Pro Thr Pro Val Lys Pro
370 375 380
Gly Thr Asn Met Asn Ile Thr Glu Gln Leu Gln Gly Thr Ile Gln Ser
385 390 395 400
Ile Val Ser Gln
<210> 7
<211> 353
<212> PRT
<213> 芽孢杆菌属的种
<400> 7
Met Val Lys Arg Asp Ile Ser Ala Arg Asp Leu Gly Gly Lys Glu Asp
1 5 10 15
Thr Pro Phe Leu Leu Lys Lys Asp Glu Trp Ile Lys Ile Gln Lys Tyr
20 25 30
Thr Gly Asp Gly Ala Tyr Leu Pro Val Asn Val Asn Glu Met Arg Lys
35 40 45
Ala Leu Leu Leu Pro Asn Thr Glu Glu Leu Pro Asp Phe Gly Glu Leu
50 55 60
Tyr Thr Val Asn Thr Ser Ile Lys Gly His Cys Lys Asn Trp Asn Asp
65 70 75 80
Thr Thr Tyr Arg Glu Ile Leu Asp Val Ala Asn Lys Ile Val Asn Tyr
85 90 95
Ser Arg Arg Ala Glu Val Phe Tyr Ala Pro Leu Leu Glu Cys Leu Pro
100 105 110
Asp Ile Leu Glu Gly Asp Thr Val Ala Leu Glu Lys Phe Gln Lys Ile
115 120 125
Cys Lys Lys Leu Ala Thr Glu Ala Lys Asp Phe Ser Asp His Ala Glu
130 135 140
Thr Leu Ala Lys Asn Val Glu Gln Phe Ala Asn Asp Thr Ala Asn Asp
145 150 155 160
Tyr Lys Asn Leu Ile Asn Val Lys Val Lys Tyr Asp Ala Leu Tyr Gly
165 170 175
Lys Asn Ser Glu Glu Val Thr Arg Leu Arg Ala Glu Val Glu Lys Leu
180 185 190
Arg Glu Glu Leu Glu Gly Tyr Ile Glu Asp Leu Glu Asp Phe Glu Glu
195 200 205
Glu Val Trp Leu Ser Leu Leu Leu Gly Pro Ile Phe Gly Phe Val Leu
210 215 220
Lys Glu Ile Leu Glu Ser Thr Lys Gly Lys Met Leu Gln Ala Lys Ile
225 230 235 240
Asp Ala Thr Arg Gln Lys Ile Glu Ala Thr Asn Ala Thr Ile Gln Arg
245 250 255
Asn Val Tyr Leu Met Ser Leu Leu Asp Lys Ala Asp Val Gly Thr Asp
260 265 270
Lys Thr Gln Asn Gln Ile Glu Glu Ala Leu Pro Val Ile Asn Lys Ile
275 280 285
Lys Ala Ile Trp Tyr Ser Leu His Thr Asp Leu Leu Thr Leu Ser Asn
290 295 300
Ile Thr Met Lys Asp Ile His Asp Asp Pro Glu Phe Ala Asp Leu Gly
305 310 315 320
Ile Glu Leu Ala Ile Met Gln Trp Val Lys Val Gly Lys Gln Ala Asp
325 330 335
Asp Phe Arg Ile Asn Ala Asp Val Gly Phe Ile Val Asp Gln Tyr Ile
340 345 350
Ala
<210> 8
<211> 494
<212> PRT
<213> 芽孢杆菌属的种
<400> 8
Met Asp Thr Lys Phe Leu Val Thr Phe Leu Lys Glu Leu Ile Asn Glu
1 5 10 15
Lys Ser Val Asn Lys Val Met Ala Ser Thr Gly Thr Gln Phe Ile Lys
20 25 30
Asn Ala Gln Lys Asn Pro Asp Asn Phe Asn Asn Thr Phe Arg Thr Leu
35 40 45
Ala Met Val Ser Ala Glu Phe Ile Pro Val Gly Gly Ile Phe Ile Ser
50 55 60
Pro Leu Ile Gly Leu Leu Trp Lys Glu Glu Gly Thr Ser Asp Gln Ile
65 70 75 80
Asn Ala Leu Arg Asn Glu Leu Ser Glu Leu Ile Asp Gln Lys Ile Glu
85 90 95
Thr Glu His Leu His Ser Leu Ala Lys Glu Phe Glu Ile Leu Glu Gly
100 105 110
Thr Ile Lys Gln Leu Glu Ser Ser Val Asn Gly His Ser Glu Glu Tyr
115 120 125
Phe Glu Ser His Gly Thr Ile Gln Glu Ser Arg Arg Asn Trp Ala His
130 135 140
Ser Ile Gln Arg Ser Phe Gln Arg Leu Ile Gly Leu Ala Ser Gln Asp
145 150 155 160
Arg His Lys Ile Ala Asp Leu Ala Leu Tyr Thr Lys Ile Ala Leu Ser
165 170 175
His Val Ser Phe Leu Asn Cys Ile His Glu Asn Gly Gln Gly Ser Lys
180 185 190
Phe Lys Tyr Asp Lys Gln Ser Leu Leu Asp Phe Phe Asn His Ser Lys
195 200 205
Leu Ser Asp Ile Ile Asp Asn Tyr Thr Lys Tyr Ile Glu Lys Thr Phe
210 215 220
Lys Glu Gly Asp Asp Lys Phe Lys Glu Lys Ile Lys His Arg Glu Ala
225 230 235 240
Leu Lys Gln Asp Leu Ile Glu Leu Arg Lys Gln Pro Ser Thr Gly Phe
245 250 255
Gly Asp Leu Leu Lys Glu Lys Lys Met Arg Asp Leu Glu Glu Gln Ile
260 265 270
Lys Asn Tyr Glu Lys Met Asp Leu Glu Asn Cys Gln Asn Lys Phe Tyr
275 280 285
Tyr Thr Thr Trp Gly Asp Glu Thr Leu Gln Ala Leu Ile Lys Gly Arg
290 295 300
Trp Thr Leu Lys Asp Gly Lys Leu Tyr Phe Val Asn Lys Asn Gly Glu
305 310 315 320
Lys Gln Thr Arg Trp Leu Gln Leu Gly Val Lys Trp Tyr Tyr Leu Ser
325 330 335
Pro Leu Glu Asn Asn Thr Asn Thr Lys Gly Glu Thr Phe Asn Ile Gly
340 345 350
Gln Met Met Ile Gly Trp Val Arg Ala Glu Asp Lys Trp Tyr Tyr Leu
355 360 365
Ser Pro Ser Asp Asn Asn Met Glu Ile Phe Asn Thr Gly Gln Met Met
370 375 380
Thr Arg Trp Ala Gln Asp Gly Asp Lys Trp Tyr Tyr Leu Ala Pro Val
385 390 395 400
Asn Asp Ala Lys Asn Tyr Asp Gly Ile Lys Phe Ala Gln Gly Gln Met
405 410 415
Met Thr Gly Trp Val Gln Ile Thr Asp Arg Asn Asp Lys Lys Asp Tyr
420 425 430
Tyr Tyr Phe Ser Pro Ser Glu Ser Thr Val Asn Tyr Asp Gly Ile Lys
435 440 445
Phe Ala Gln Gly Gln Met Met Val Gly Trp Val Lys Val Ser Gly Lys
450 455 460
Trp Tyr Tyr Leu Asp Lys Lys Asp Gly Tyr Met Leu His Asp Thr Ile
465 470 475 480
Thr Pro Asp Gly Tyr Arg Val Asp Glu Gln Gly Val Leu Lys
485 490
<210> 9
<211> 1146
<212> PRT
<213> 芽孢杆菌属的种
<400> 9
Leu Phe Met Ile Thr Ser Glu Tyr Glu Gly Asp Asn Lys Met Asn Pro
1 5 10 15
Asn Glu Glu Tyr Lys Thr Thr Val Pro His Ser Asn Lys Ile Gln Pro
20 25 30
Arg Tyr Pro Leu Thr Asn Asn Pro Asp His Pro Leu Gln His Thr Asn
35 40 45
Tyr Lys Asp Trp Leu Gln Met Cys Gln Asn Thr Ser Phe Asp Gly Glu
50 55 60
Asn Pro Val Pro Ser Val Thr Glu Val Gly Ala Ile Gly Ile Ala Ile
65 70 75 80
Ser Ile Val Ser Thr Leu Leu Ser Thr Phe Gly Ala Ala Val Pro Gly
85 90 95
Ala Ala Leu Ala Val Leu Gly Ala Thr Leu Pro Phe Phe Asp Ser Gly
100 105 110
Ser Gly Ser Val Trp Glu Asp Phe Met Ile His Asn Ser Ser Val Leu
115 120 125
Gln Lys Thr Leu Pro Glu Ser Ile Lys Asn Gln Ala Asn Thr Ile Leu
130 135 140
Thr Gly Leu Gln Glu Ser Leu Ile Glu Tyr Lys Arg Val Phe Asn Ile
145 150 155 160
Trp Lys Lys Asp Lys Thr Pro Ala His Ala Glu Gln Val Arg Ser Thr
165 170 175
Tyr Arg Asn Thr His Asn Leu Phe Ile Asn Arg Met Ser Glu Leu Arg
180 185 190
Pro Ser Gln Tyr Lys Gln Ile Leu Leu Ser Val Tyr Cys His Ala Ala
195 200 205
Asn Leu His Leu Gly Leu Leu Val Gln Gly Val Ala Tyr Tyr Asn Glu
210 215 220
Trp Tyr Asn Val Asn Pro Ser Leu Leu Thr Pro Asp Arg Thr Val Asp
225 230 235 240
Asp Glu Tyr Leu Leu Leu Gln Asp Lys Ile Lys Glu Tyr Ile Asp Tyr
245 250 255
Cys Ser Lys Thr Tyr Arg Glu Gly Leu Asn Ile Leu Ala Asn Ser Gln
260 265 270
Ala Asn Leu Lys Ile Tyr Thr Thr Tyr Gln Arg Glu Met Thr Phe Thr
275 280 285
Val Leu Asp Ile Ile Thr Asn Phe Pro Tyr Tyr Asp Phe Lys Lys Tyr
290 295 300
Pro Ile Gly Val Gln Ser Glu Leu Thr Arg Glu Ile Tyr Thr Ile Glu
305 310 315 320
Gln Gln Ile Lys Gln Ile Tyr Leu Asn His Leu Thr Arg Gln Pro Asn
325 330 335
Leu Val Thr His Pro Phe Asn Ser Ser Ile Tyr His Tyr Arg Asn Leu
340 345 350
Pro Phe Ile Thr Ser Gly Phe Thr Ala Val Arg Asn Arg Phe Trp Phe
355 360 365
Thr Asn Ala Arg Asn Leu Ser Val Ser Gly Leu Tyr Gly Ser Thr Ser
370 375 380
Phe Asp Gly Gln Phe Asp Val Ser Thr Gln Asn Val Trp Lys Val Glu
385 390 395 400
Ala Thr Tyr Ala Asp Val Ala Arg Arg Leu Lys Gln Leu Lys Phe Tyr
405 410 415
Ala Gln Leu Pro Asn Pro Ile Thr Ala Thr Thr Gly Thr Pro Asn Asn
420 425 430
Ser Asp Tyr Arg Glu Leu Phe Thr His Glu Ile Pro Gly Arg Asp Gly
435 440 445
Lys Leu Pro Thr Thr Phe Gln Thr Ala Ser His Tyr Leu Ser Tyr Met
450 455 460
Met Met Thr Ala Ser Glu Ser Pro Gly Leu Ser Cys Ala Trp Arg His
465 470 475 480
Ala Ser Val Asn Asn Asp Asn Leu Ile Ser Pro Asn Lys Ile Thr Leu
485 490 495
Ile Pro Ala Val Lys Ala Arg Glu Leu Asp Thr Ser Ser Lys Val Val
500 505 510
Ser Gly Pro Gly His Thr Gly Gly Asp Leu Val Glu Phe Asn Met Ala
515 520 525
His Gly Arg Leu Glu Leu Gln Gly Ser Ile Gly Asn Val Ser Gln Glu
530 535 540
Arg Phe Thr Ile Arg Leu Arg Tyr Ala Ala Ala Ala Asn Glu Ser Pro
545 550 555 560
Arg His Leu Leu Val Thr Ile Pro Asn Ile Gly Val Phe Pro Val Met
565 570 575
Leu Leu Pro Thr Thr Tyr Lys Leu Ser Leu Asn Glu Phe Ser Tyr Asn
580 585 590
Glu Phe Gly Phe Val Asp Ile Ile Pro Ser Asn Glu Ser Ile Ser Phe
595 600 605
Phe Ala Ser Ile Pro Trp Ile Val Ser Ile Thr Ser Thr Arg Gly Ser
610 615 620
Ile Asp Asp Lys Val Tyr Ile Asp Arg Leu Glu Phe Ile Pro Ile Ser
625 630 635 640
Arg Leu Pro Asn Arg Gln Arg Gln Gln Leu Glu Lys Thr Gln Gln Val
645 650 655
Val Asp Gly Leu Phe Thr Ser Ser Glu Lys Gln Glu Ile Lys Met Ser
660 665 670
Thr Thr Asp Phe Arg Ile Asp Gln Thr Ser Thr Leu Val Glu Cys Leu
675 680 685
Ser Asp Thr Glu Tyr Pro Gln Glu Gln Leu Leu Leu Leu Asp Gln Val
690 695 700
Lys Tyr Ala Lys Gln Leu Ser Gln Ser Arg Asn Leu Leu Ser Asn Thr
705 710 715 720
Phe Gly Asn Gln Gly Trp Ser Thr Gln Gly Asp Val Val Leu Ser Ser
725 730 735
Gly Gly Ser Leu Phe Lys Asp Ser Tyr Phe Ser Leu Pro Gly Ala Arg
740 745 750
Thr Thr Gln Val Gly Pro Asp Thr Phe Pro Thr Tyr Met Tyr Gln Lys
755 760 765
Ile Asp Glu Ser Gln Leu Lys Pro Tyr Thr Arg Tyr Lys Val Arg Gly
770 775 780
Phe Ile Glu Ala Ser Asn His Leu Glu Val Ala Ile Ser Arg Tyr Gly
785 790 795 800
Asn Glu Val Lys Thr Met Leu His Val Pro Asp Asp Leu Ala Thr Ser
805 810 815
Ser Thr Arg Asp Asn Cys Gly Glu Trp Asn Arg Cys Glu Thr Thr Gln
820 825 830
His Phe Phe Thr Glu Asn Thr Glu Glu Ile Ser Lys Pro Ala Val Ser
835 840 845
Ser Thr Thr Cys Ser Pro Cys Asp Ala Thr His Thr Ser His Lys Thr
850 855 860
Cys Ile Asp Ser His Asp Phe Ser Phe Thr Ile His Thr Gly Asp Ile
865 870 875 880
Asp Ile Thr Asn Lys Pro Gly Ile His Ile Leu Phe Lys Ile Ser Asp
885 890 895
Pro Asp Gly Phe Ala Thr Leu Gly Asn Leu Glu Val Ile Glu Asp Gly
900 905 910
Pro Ile Phe Glu Asp Thr Ile Ser Thr Val Lys Tyr Gln Glu Gln Arg
915 920 925
Trp Lys Gln Asn Leu Ile Glu Gln Gln Gln Lys Thr Glu Gln Ala Phe
930 935 940
Leu Gln Val Gln Gln Ala Val Asp Ser Leu Phe Gln Asp Ser Gln Tyr
945 950 955 960
Gln Thr Leu Gln Leu Glu Val Thr Ala Ser Gln Ile His Ser Ala Glu
965 970 975
Asn Leu Leu Gln Glu Ile Ser Ser Met His Asn Asp Ile Gln Ser Pro
980 985 990
Lys Asn Glu Glu Arg Phe Thr Ala Leu Asn Asn Gln Ile Gln Gln Ala
995 1000 1005
Tyr His Leu Tyr Ser Lys Arg Asn Met Val Arg Asn Gly Asp Phe
1010 1015 1020
Ser Glu Gly Leu His Tyr Trp Ser Ala Ser Ser Asp Ala Ser Ile
1025 1030 1035
Gln Gln Thr Gln Asp Asp Ser Val Leu Leu Ile Pro Ser Trp Ser
1040 1045 1050
Thr Gln Val Ser Gln Gln Leu Arg Leu Gln Pro Asn His Arg Tyr
1055 1060 1065
Leu Leu Arg Val Ala Ala Leu Lys Glu His Ile Gly Lys Gly Trp
1070 1075 1080
Ile Lys Ile Ser Asp Cys Lys His Gln Val Gln Thr Ile His Phe
1085 1090 1095
Ser Ser Cys Asp Glu Asn Glu Ile His His Ser Ile Tyr Gln Thr
1100 1105 1110
Lys Ser Ile Pro Phe Thr Pro Thr Thr Asp Gln Ile Arg Ile Asp
1115 1120 1125
Ile Gly Glu Thr Glu Gly Ser Phe Thr Ile Arg Ser Ile Glu Leu
1130 1135 1140
Ile Cys Leu
1145
<210> 10
<211> 639
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 10
Leu Phe Met Ile Thr Ser Glu Tyr Glu Gly Asp Asn Lys Met Asn Pro
1 5 10 15
Asn Glu Glu Tyr Lys Thr Thr Val Pro His Ser Asn Lys Ile Gln Pro
20 25 30
Arg Tyr Pro Leu Thr Asn Asn Pro Asp His Pro Leu Gln His Thr Asn
35 40 45
Tyr Lys Asp Trp Leu Gln Met Cys Gln Asn Thr Ser Phe Asp Gly Glu
50 55 60
Asn Pro Val Pro Ser Val Thr Glu Val Gly Ala Ile Gly Ile Ala Ile
65 70 75 80
Ser Ile Val Ser Thr Leu Leu Ser Thr Phe Gly Ala Ala Val Pro Gly
85 90 95
Ala Ala Leu Ala Val Leu Gly Ala Thr Leu Pro Phe Phe Asp Ser Gly
100 105 110
Ser Gly Ser Val Trp Glu Asp Phe Met Ile His Asn Ser Ser Val Leu
115 120 125
Gln Lys Thr Leu Pro Glu Ser Ile Lys Asn Gln Ala Asn Thr Ile Leu
130 135 140
Thr Gly Leu Gln Glu Ser Leu Ile Glu Tyr Lys Arg Val Phe Asn Ile
145 150 155 160
Trp Lys Lys Asp Lys Thr Pro Ala His Ala Glu Gln Val Arg Ser Thr
165 170 175
Tyr Arg Asn Thr His Asn Leu Phe Ile Asn Arg Met Ser Glu Leu Arg
180 185 190
Pro Ser Gln Tyr Lys Gln Ile Leu Leu Ser Val Tyr Cys His Ala Ala
195 200 205
Asn Leu His Leu Gly Leu Leu Val Gln Gly Val Ala Tyr Tyr Asn Glu
210 215 220
Trp Tyr Asn Val Asn Pro Ser Leu Leu Thr Pro Asp Arg Thr Val Asp
225 230 235 240
Asp Glu Tyr Leu Leu Leu Gln Asp Lys Ile Lys Glu Tyr Ile Asp Tyr
245 250 255
Cys Ser Lys Thr Tyr Arg Glu Gly Leu Asn Ile Leu Ala Asn Ser Gln
260 265 270
Ala Asn Leu Lys Ile Tyr Thr Thr Tyr Gln Arg Glu Met Thr Phe Thr
275 280 285
Val Leu Asp Ile Ile Thr Asn Phe Pro Tyr Tyr Asp Phe Lys Lys Tyr
290 295 300
Pro Ile Gly Val Gln Ser Glu Leu Thr Arg Glu Ile Tyr Thr Ile Glu
305 310 315 320
Gln Gln Ile Lys Gln Ile Tyr Leu Asn His Leu Thr Arg Gln Pro Asn
325 330 335
Leu Val Thr His Pro Phe Asn Ser Ser Ile Tyr His Tyr Arg Asn Leu
340 345 350
Pro Phe Ile Thr Ser Gly Phe Thr Ala Val Arg Asn Arg Phe Trp Phe
355 360 365
Thr Asn Ala Arg Asn Leu Ser Val Ser Gly Leu Tyr Gly Ser Thr Ser
370 375 380
Phe Asp Gly Gln Phe Asp Val Ser Thr Gln Asn Val Trp Lys Val Glu
385 390 395 400
Ala Thr Tyr Ala Asp Val Ala Arg Arg Leu Lys Gln Leu Lys Phe Tyr
405 410 415
Ala Gln Leu Pro Asn Pro Ile Thr Ala Thr Thr Gly Thr Pro Asn Asn
420 425 430
Ser Asp Tyr Arg Glu Leu Phe Thr His Glu Ile Pro Gly Arg Asp Gly
435 440 445
Lys Leu Pro Thr Thr Phe Gln Thr Ala Ser His Tyr Leu Ser Tyr Met
450 455 460
Met Met Thr Ala Ser Glu Ser Pro Gly Leu Ser Cys Ala Trp Arg His
465 470 475 480
Ala Ser Val Asn Asn Asp Asn Leu Ile Ser Pro Asn Lys Ile Thr Leu
485 490 495
Ile Pro Ala Val Lys Ala Arg Glu Leu Asp Thr Ser Ser Lys Val Val
500 505 510
Ser Gly Pro Gly His Thr Gly Gly Asp Leu Val Glu Phe Asn Met Ala
515 520 525
His Gly Arg Leu Glu Leu Gln Gly Ser Ile Gly Asn Val Ser Gln Glu
530 535 540
Arg Phe Thr Ile Arg Leu Arg Tyr Ala Ala Ala Ala Asn Glu Ser Pro
545 550 555 560
Arg His Leu Leu Val Thr Ile Pro Asn Ile Gly Val Phe Pro Val Met
565 570 575
Leu Leu Pro Thr Thr Tyr Lys Leu Ser Leu Asn Glu Phe Ser Tyr Asn
580 585 590
Glu Phe Gly Phe Val Asp Ile Ile Pro Ser Asn Glu Ser Ile Ser Phe
595 600 605
Phe Ala Ser Ile Pro Trp Ile Val Ser Ile Thr Ser Thr Arg Gly Ser
610 615 620
Ile Asp Asp Lys Val Tyr Ile Asp Arg Leu Glu Phe Ile Pro Ile
625 630 635
<210> 11
<211> 178
<212> PRT
<213> 芽孢杆菌属的种
<400> 11
Val Glu Arg Thr Met Glu His Lys Lys Ile Asp Ala Pro Ser Ile Ser
1 5 10 15
Tyr Ala Ser Leu Ser Gly His Pro Val Tyr Asp Lys Asn His Ile Leu
20 25 30
Ser Phe Ser Asn Ile Ile Asn Ile Pro Lys Gly Phe Gln Ile Thr Thr
35 40 45
Gln Asn Lys Leu Asn Phe Ser Tyr His Val Ser Leu Leu Ser Met Val
50 55 60
Gln Glu Asn Cys Lys Arg Ile Val Glu Ile Glu Asp Cys Gly Gln Leu
65 70 75 80
Glu Val Asp Leu Gln Ile Leu Lys Ile Lys Gly Ser Ile Pro Tyr Ile
85 90 95
Val Asn Leu Tyr Leu Glu Pro Ile Pro Pro Thr Asn His Cys Val His
100 105 110
Ile Leu Leu Glu Asp Pro Ile Ala Leu Ser Tyr Gln Asp Thr Leu Tyr
115 120 125
Val Asp Gln Val Leu Leu Cys Ser Thr Arg Lys Leu Pro Tyr Tyr Val
130 135 140
Ile Asp Thr Glu His Ile Gln Ile Lys Asn Leu Gln Val Glu Thr Val
145 150 155 160
Glu Pro Gln Gln Leu Val Ala Lys Val Lys Gly Glu Phe His Leu Asp
165 170 175
Tyr Gln
<210> 12
<211> 346
<212> PRT
<213> 芽孢杆菌属的种
<400> 12
Met Lys Lys Tyr Lys Asn Arg Ser Phe Thr Met Asn Asn Lys Lys Asn
1 5 10 15
Met Lys Arg Lys Ile Ala Ala Thr Ser Ser Ile Ile Thr Leu Ala Thr
20 25 30
Ser Phe Ala Phe Thr Ser Pro Glu Leu Val Lys Ala Asp Ser Lys Asn
35 40 45
Ser Val Ser Lys Asn Gln Gln Tyr Met Ala Ala Glu Glu Arg Pro Thr
50 55 60
Val Pro Val Ser Thr Asp Glu Asp Tyr Gly Ile Arg Ser Leu Ala Ile
65 70 75 80
Asp Asn Lys Asp Thr Phe Tyr Asp Val Val Arg Lys Ile Val Gly Trp
85 90 95
Asn Gly Ile Gln Asp Leu Ser Ala Asn Phe Leu His Tyr Ala Lys Pro
100 105 110
Thr Tyr Ser Val Asp Leu Val Ala Glu Pro Lys Asp Glu Pro Leu Ser
115 120 125
Pro Val Leu Ile Gly Lys Thr Asn Ile Gln Asn Lys Ser Gln Ser Thr
130 135 140
Gln Lys Leu Gln Ser Ala Ser Met Ser Lys Thr Val Thr Ser Thr Val
145 150 155 160
Thr Ser Thr Val Thr His Gly Ala Lys Ala Gly Ala Lys Ala Ser Ala
165 170 175
Lys Leu Lys Val Pro Leu Val Ala Glu Ala Gly Val Glu Leu Asn Thr
180 185 190
Glu Tyr Asn Phe Ala Ser Ala Gly Ser Asn Thr Ser Thr Glu Thr Leu
195 200 205
Thr Tyr Thr Leu Pro Ser Gln Ser Ile Glu Leu Glu Pro Gly Gln Arg
210 215 220
Ala Thr Val Thr Ser Asp Leu Ser Met Met Lys Thr Ser Gly Glu Val
225 230 235 240
Gln Leu Arg Thr Lys Tyr Glu Gly Tyr Val His Leu Lys Gly Thr Leu
245 250 255
Ile Asn Thr Pro Asn Asn Ile Asp Asn Asn Ile Lys Met Gly Asp Phe
260 265 270
Val Asp Arg Phe Ile Lys Ala Asp Ser Ser Leu Arg Glu Tyr Trp His
275 280 285
Val Asn Ser Pro Asn Asp Phe Ile Ala Tyr Gln Val Gly Lys Gly Asn
290 295 300
Phe Thr Ala Glu Tyr Gly Thr Val Ala Asn Ile Thr Val Glu Ile Phe
305 310 315 320
Asn Gln Asp Gly Thr Asp Thr Thr Gln Pro Ile Lys Ser Tyr Thr Tyr
325 330 335
Thr Val Ile Pro Glu Val Ser Lys Asn Ile
340 345
<210> 13
<211> 258
<212> PRT
<213> 链霉菌属的种(Streptomyces sp.)
<400> 13
Val Thr Leu Ile Ser Ala Leu Ala Arg Pro Pro Ala Asp Pro Val Gly
1 5 10 15
Asp Gly Gly Asn Gly Asp Asp Arg Thr Pro Gly His Arg Arg Gly Ser
20 25 30
Pro Gly Thr Val Val Ser Gly Pro Ser Ser Ser Ala Arg Arg Ser Leu
35 40 45
Arg His Thr Asp Pro Ser Leu Ile Asn Arg Leu Phe Lys Glu Asp Ser
50 55 60
Leu Ala Ala Ser Phe Lys Thr Val Ile Glu Val Gly Pro Ala His Leu
65 70 75 80
Asp Gln Ala Gln Ala Leu Gly Arg Ala Phe Gln Asp Ala Ile Ala Pro
85 90 95
Ala Thr Val Asn Tyr Asp Phe Asp His Ile Arg Asp Ala Ala Ala Ala
100 105 110
Ile Pro Asp Ser Ser Ile Val Lys Met Val Arg Gly Trp Gly Leu Gln
115 120 125
Glu His Ala Pro Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val
130 135 140
Arg Gln Ala Leu Pro Pro Glu Ser Ala Glu Ala Ser Phe Trp Asp Thr
145 150 155 160
Val Glu Arg Glu Leu Val Asp Ala Phe Thr Gly Leu Ala Ala Gln Glu
165 170 175
Gly Leu Pro Gly Leu Ser Tyr Tyr Glu Glu Gly Ala Asp Arg Thr Arg
180 185 190
Tyr Tyr Arg Asp Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Gly
195 200 205
His Met Tyr Ala Ile Ala Phe Cys Ile Asp Val Thr Ile Gly Leu Asp
210 215 220
Lys Ala Arg Val Gly Ala Leu Glu Met Thr Asp Ala Val Pro Phe Ala
225 230 235 240
Leu Arg Leu Asn Ala Ile Val Leu Arg Gln Glu Leu Thr Arg Ala Leu
245 250 255
Thr Ala
<210> 14
<211> 304
<212> PRT
<213> 芽孢杆菌属的种
<400> 14
Met Thr Asp Thr Lys Met Thr Ile Glu Tyr Leu Phe Thr Pro Tyr Tyr
1 5 10 15
Gly Val Asn Pro Asn Gly Ser Asn Ile Asn Pro Tyr Leu Leu Asn Ala
20 25 30
Asn His Trp Asn Ser Val Met Cys Tyr Phe Asn Tyr Ile Ser Pro Tyr
35 40 45
Thr Glu Ile Arg Asn Arg Asp Thr Leu Gly Lys Leu Leu Tyr Ser Ser
50 55 60
Val Asn Gln Gln Gln Ala Phe Tyr Arg Phe Phe Asp Val Tyr Asn Leu
65 70 75 80
Gln Asp Val Phe Gln Pro Ile Phe Asp Leu His Arg Thr Ile Ser Asn
85 90 95
Ala Ser Thr Trp Trp Thr Ile Gln Gly His Gln Gln Ile Thr Leu Leu
100 105 110
Val Gln Asp Ile Arg Ser Phe Thr Asn Ile Leu Cys Val Arg His Ile
115 120 125
Asp Lys Leu Asn Gln Ile Ile Lys Asp Val Gln Lys Glu Arg Val Ser
130 135 140
Thr Lys Thr Lys Asp Gln Phe Thr Asp Ile Cys Ile Glu Leu Gln Ala
145 150 155 160
Arg Thr Ser Gly Tyr Tyr Gln Arg Leu Asp Ile Leu Asp Gln Ser Lys
165 170 175
Lys Phe Val Asn Ile Leu Leu Lys Tyr Asn Pro Ala Ile His Thr Thr
180 185 190
Leu Asn His Leu Glu Lys Ile Tyr Ser Asp Ser Glu Phe Lys Ala Val
195 200 205
Asp Lys His Tyr Phe Lys Lys Leu Leu Ser Phe Val Arg Lys Gln Gln
210 215 220
Ile Phe Val Gln Tyr Tyr Thr Tyr Thr Ile Gly Thr Leu Gln Ala Leu
225 230 235 240
Thr Asn Leu Arg Gly Ile Trp Thr Glu Tyr Gly Leu Asn Leu Gln Lys
245 250 255
Leu Ile Glu Ala Cys Asp Asn Asn Leu Val Tyr Phe Asn Ser Leu Leu
260 265 270
Ala Arg Leu Lys Leu Glu Asn Ala Ile Asn Ala Trp Gln Asp Val Lys
275 280 285
Lys Lys Ala Asp Arg Phe Leu Gln Thr Trp Gln Asp Asp Thr Arg Cys
290 295 300
<210> 15
<211> 391
<212> PRT
<213> 芽孢杆菌属的种
<400> 15
Met Lys Ser Val Ser Lys Lys Val Met Thr Gly Leu Phe Ala Gly Ala
1 5 10 15
Met Gly Leu Ser Ile Phe Ala Pro Ala Ser Gln Ala Ala Thr Pro Glu
20 25 30
Gln Asn Glu Tyr Tyr Asn Ile Lys Leu Lys Ala Asn Thr Tyr Lys Ile
35 40 45
Trp Asp Val Asp Asn Ala Ser Ser Asp Asn Asp Arg Val Ile Arg Leu
50 55 60
Trp Gly Gly Ser Thr Thr Glu Ala Asn Arg Gln Phe Val Phe Phe Pro
65 70 75 80
Leu Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro
85 90 95
Val Thr Phe Gly Asn Gly Ala Trp Phe Ser Ile Asn Gly Gly Ser Ser
100 105 110
Ile Leu Ala Gly Ala Asp Ile Leu Gln Gln Gln Ser Trp Thr Gly Ser
115 120 125
Ala Asn Gln Gln Trp Tyr Leu Arg Asp Gln Gly Asn Asn Asn Tyr Glu
130 135 140
Ile Val Asn Gln Gly Asn Gly Lys Val Ala Ser Tyr Ala Trp Gly Gly
145 150 155 160
Thr Leu Ala Gln Asn Trp Glu Tyr Val Asp Leu Asp Glu Ser Lys Pro
165 170 175
Tyr Asp Ser Asn Asn Val Phe Asn Ile Ala Asn Ser Gly Ser Ser Ile
180 185 190
Ser Leu Pro Asn Leu Pro Ala Lys Gly Asp Arg Pro Asn Ala Pro Asp
195 200 205
Tyr Asn Gln Thr Gly Arg Ile Asp Gln Gln Leu Pro Gln Thr Ser Glu
210 215 220
Ser Val Val Ile Gly Ala Ser Leu Ile Pro Ser Ile Met Val Lys Asp
225 230 235 240
Asn Ser Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Val
245 250 255
Leu Glu Lys Glu Glu Tyr Trp Glu Lys Val Arg Ser Glu Ile Val Pro
260 265 270
Ala Gly Gly Ser Ser Lys Tyr Thr Val Lys Arg Gly Val Ser Thr Val
275 280 285
Asp Gln Gln Lys Met Thr Asp Thr Leu Asn Met Lys Phe Gly Thr Asp
290 295 300
Leu Gly Phe Lys Phe Gly Asp Thr Thr Ala Ser Ile Lys Ser Glu Ile
305 310 315 320
Ser Lys Thr Leu Gln Thr Glu Val Ser Lys Thr Ser Thr Asp Ala Thr
325 330 335
Glu Glu Thr Lys Glu Lys Gly Phe Ser Ser Thr Ser Gly Lys Asp Thr
340 345 350
Gly Leu Thr Val Tyr Gln Leu Val Thr Lys Tyr Thr Leu Lys Arg Thr
355 360 365
Asp Gly Ser Thr Val Ser Ser Ser Tyr Val Arg Asp Gly Asp Gln Thr
370 375 380
Leu Glu Arg Thr Ile Ala Lys
385 390
<210> 16
<211> 325
<212> PRT
<213> 乳球菌属的种(Lactococcus sp.)
<400> 16
Met Lys Lys Asn Lys Lys Tyr Val Met Val Ile Ser Cys Arg Leu Leu
1 5 10 15
Ile Leu Ser Phe Gly Met Val Pro Lys Asn Val Leu Ala Leu Asn Gln
20 25 30
Gly Gly Ala Gly Asn Ser Gly Ser Gly Tyr Leu Val Asn Asn Met Ser
35 40 45
Glu Lys His Glu Asp Ile Ile Asp Pro Glu Asp Ile Phe Ser Asn Met
50 55 60
Leu Asn Ala Val Gly Lys Tyr Lys Glu His Lys Asp Pro Phe Leu Phe
65 70 75 80
Val Thr Arg Asn Ser Ile Gly Ser Leu Ser Ser Tyr Ser Asn Phe Asn
85 90 95
Ala Glu Gln Asp Gly Asp Pro Ile Ile Glu Asn Glu Ser Leu Leu Phe
100 105 110
Val Gly Glu Ser Ile Leu Thr Asn Asn Met Asn Thr Glu Gln Thr Leu
115 120 125
Lys Thr Asn Ser Phe Ser Lys Thr Met Ser Asn Thr Val Ser Ser Gln
130 135 140
Val Thr His Gly Phe Met Leu Gly Thr Glu Ala Arg Thr Ser Phe Gly
145 150 155 160
Ile Pro Leu Ile Gly Glu Thr Glu Leu Ser Leu Lys Ala Glu Tyr Asn
165 170 175
Phe Ser Ser Ser Glu Thr Asn Glu Ile Ser Glu Ser Tyr Thr Tyr Gly
180 185 190
Val Glu Pro Gln Glu Ile Leu Val Pro Pro His Ser Ser Val Lys Val
195 200 205
Arg Val Asp Leu Asn Thr Ala Asp Ile Ser Gly Lys Val Lys Leu Met
210 215 220
Ala Thr Pro Lys Thr Asp Phe Gly Asp Ile Asp Gly Tyr Phe His Tyr
225 230 235 240
Gly Pro Asp Glu Gln Asn Ile Ala Glu Ser Ile Pro Phe Thr Tyr Asn
245 250 255
Trp Asn Thr Leu Thr Lys Glu Val Arg Gln Leu Asn Leu Pro Val Asp
260 265 270
Asp Ile Asp Tyr Asp Phe Asn Asn Gln Thr Pro Lys Leu Val Gly Ser
275 280 285
Gly Lys Tyr Lys Ala Lys Tyr Gly Thr Thr Phe Lys Val Glu Val Ile
290 295 300
Pro Ile Asn Asn Pro Lys Ala Glu Gly Tyr Ser Tyr Ser Ile Gln Pro
305 310 315 320
Thr His Thr Ala Asn
325
<210> 17
<211> 546
<212> PRT
<213> 芽孢杆菌属的种
<400> 17
Met Lys Glu Lys Lys Asn Ile Gln Glu Arg Ile Gly Asp Asn Val Lys
1 5 10 15
Tyr Asn Glu Arg Lys Lys Ala Lys Arg Asn Tyr Lys Lys Ala Val Leu
20 25 30
Thr Ala Ala Thr Ala Phe Ser Ile Gly Val Thr Ser Ile Gly Gly Ala
35 40 45
Thr Ser Ala Phe Ala Ala Glu Lys Pro Pro Ala Gln Gln Gln Pro Gln
50 55 60
Ser Ser Val Lys Ser Val Gly Gly Ala Asp Asp Val Tyr Ser Asn Glu
65 70 75 80
Ile Asn Asn Pro Pro Phe Lys Ile Glu Gly Asp Ala Leu Thr Leu Thr
85 90 95
Lys Asn Phe Leu Ser Gly Asp Leu Ala Ala Thr Ala Lys Lys Met Trp
100 105 110
Gly Ser Ser Leu Asn Leu Ala Val Gln Ser Ala Gln Gly Lys Asn Val
115 120 125
Leu Asn Val Asn Asn Leu Ala Arg Asp Met Val Met Val Gly Thr Ser
130 135 140
Ala Ile Pro Tyr Gly Gly Val Ile Leu Ser Ser Ile Ile Gly Val Leu
145 150 155 160
Trp Pro Met Ser Gly Thr Asn Gln Met Gln Ala Leu Met Asp Lys Met
165 170 175
Gly Gln Ile Met Asp Gln Lys Ile Glu Lys Tyr Asp Asn Thr Thr Leu
180 185 190
Thr Ser Lys Ile Asn Ala Val Glu Val Ala Leu Lys Arg Phe Glu Thr
195 200 205
Ile Leu Tyr Gly Thr Asp Asp Val Tyr Ser Asp Thr Asn Lys Ala Gly
210 215 220
Glu Ala Leu Arg Gln Ala Thr Val Ile Gln Asn Ala Phe Thr Glu Leu
225 230 235 240
Ile Asn Glu Ser Lys Lys Glu Glu Tyr Glu Thr Ser Glu Leu Pro Ala
245 250 255
Tyr Thr Met Ala Ala Thr Ala His Leu Leu Phe Leu Ser Phe Met Val
260 265 270
Thr Asn Gly Thr Ser Ile Leu Gly Leu Glu Pro Asp Thr Met Lys Asn
275 280 285
Ser Tyr Asn Asn Asp Leu Lys Asn Ala Leu Ser Glu Tyr Asn Ser His
290 295 300
Ile Gln Arg Ile Gly Ser Asp Ser Asp Tyr Lys Leu Ser Gln Leu Pro
305 310 315 320
Gln Lys Ile Asp Ala Ala Arg Val Glu Ile Glu Lys Ala Lys Ala Gly
325 330 335
Met Asn Pro Pro Ile Gly Ala Ala Ala Gly Tyr Trp Ala Glu Ile Tyr
340 345 350
Gln Gln Lys Ser Gln Glu Leu His Asn Leu Leu Glu Gln Gln Lys Gln
355 360 365
Leu Thr Asp Leu Val Asn Ser Thr Thr Lys Asn Glu Ala Phe Ala Ala
370 375 380
Ala Leu Lys Ser Ala Lys Thr Thr Ala Asp Asn Lys Gly Ile Lys Tyr
385 390 395 400
Glu Glu Lys Asp Ser Ser Lys Ile Val Asp Gly Gly Thr Tyr Lys Ile
405 410 415
Ile Ser Ala Leu Asn Asn Gly Gln Tyr Val Asp Asn Gln Arg Glu Thr
420 425 430
Lys Asn Val Ser Ile Tyr His Asn Thr Arg Ser Asp Asn Gln Asn Trp
435 440 445
Lys Phe Ile Phe Asp Ser Ser Lys Gln Ala Tyr Lys Ile Gln Ser Val
450 455 460
Tyr Asp Ser Arg Val Leu Ala Thr Ser Ser Ser Ala Asn Tyr Asn Val
465 470 475 480
Phe Thr Tyr Asn Phe Asp Gly Ser Glu Asp Gln Phe Trp Ile Pro Glu
485 490 495
Asp Ile Gly Asn Gly Thr Tyr Ile Phe Lys Asn Lys Lys Tyr Pro Asp
500 505 510
Arg Val Leu Asp Ile Phe Glu Gly Lys Ala Glu Asp Ser Thr Asn Ile
515 520 525
Thr Ala Tyr Pro Arg Asn Asn Gly Ala Gly Gln Lys Phe Lys Val Ile
530 535 540
Gln Gln
545
<210> 18
<211> 432
<212> PRT
<213> 芽孢杆菌属的种
<400> 18
Leu Val Leu Ser Phe Tyr Ile Ala Lys Val Ile Leu Phe Arg Asn Lys
1 5 10 15
Ser Ile Ser Asn Val Gln Tyr Asn Phe Ile Phe Cys Asn Leu Val Gly
20 25 30
Ile Leu Pro Thr Lys Leu Gly Gly Asn Ile Met Lys Ser Ile Ser Lys
35 40 45
Lys Val Met Ala Gly Leu Gly Val Gly Ala Met Ser Leu Ser Ile Trp
50 55 60
Ala Pro Ser Ser Gln Ala Ala Ala Pro Ala Asn Asn Glu Tyr His Thr
65 70 75 80
Ile His Leu Ala Ala Asn Thr Asn Ile Ala Trp Asp Val Tyr Lys Gly
85 90 95
Trp Gln Thr Asp Asp Ala Gly Ile Val Leu Trp Asp Gly Val Arg Gly
100 105 110
Asp Asn Glu Gln Phe Val Phe Phe Pro Leu Asp Gly Gly Ala Tyr Ala
115 120 125
Ile Val Asn Lys Asn Ser Gly Lys Pro Val Gly Ile Gly Ser Ala Ile
130 135 140
Ile Gly Ser Asp Gly Val Arg Asn Phe Pro Ser Tyr Asn Asn Gln Gly
145 150 155 160
Leu Arg Gln Thr Ser Trp Thr Gly Ala Pro Asn Glu Gln Trp Tyr Leu
165 170 175
Arg Asp Lys Gly Asn Asn Asn Phe Glu Ile Val Asn Gln Gly Ser Gly
180 185 190
Lys Val Ala Ser Trp Ala Gly Val Glu Met Tyr Gly Phe Lys Asp Tyr
195 200 205
Val Asp Leu Asp Glu Ser Asn Pro Ser Asp Lys Asp Arg Leu Phe Asn
210 215 220
Ile Pro Ala Ala Arg Ser Thr Phe Ser Leu Pro Thr Leu Pro Thr Val
225 230 235 240
Gly Thr Arg Pro Asn Ala Pro Asp Tyr Asn Thr Ser Gly Ala Ile Asp
245 250 255
Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu
260 265 270
Ile Pro Ser Ile Met Val Lys Asp Asn Gly Ala Ser Asp Tyr Thr Lys
275 280 285
Ile His Asn Ser Pro Tyr Tyr Val Leu Val Lys Glu Glu Tyr Trp Glu
290 295 300
Lys Val Arg Ser Glu Ile Ile Pro Ala Gly Ala Thr Ser Lys Tyr Thr
305 310 315 320
Val Lys Ser Gly Val Ser Glu Ile Asp Gln Lys Lys Met Ser Asp Thr
325 330 335
Leu Ala Met Ser Phe Gly Ser Asp Leu Gly Leu Lys Phe Gly Glu Ser
340 345 350
Ser Leu Ser Ile Lys Ser Ser Val Ser Arg Thr Ile Gln Thr Glu Val
355 360 365
Thr Lys Thr Ser Thr Asn Ala Thr Glu Glu Thr Glu Glu Lys Ser Ile
370 375 380
Pro Ser Ile Pro Gly Lys Asn Thr Gly Phe Thr Ala Tyr Gln Leu Val
385 390 395 400
Thr Lys Tyr Thr Leu Lys Arg Thr Asp Gly Ser Val Val Thr Asp Pro
405 410 415
Ser Ile Val Arg Asp Ser Lys Gln Thr Ile Ala Arg Thr Ile Ala Lys
420 425 430
<210> 19
<211> 420
<212> PRT
<213> 芽孢杆菌属的种
<400> 19
Val Ile Leu Phe Arg Asn Thr Ser Ile Ser Asn Val Gln Cys Thr Ile
1 5 10 15
Ile Phe Cys Asn Leu Val Gly Ile Leu Pro Thr Lys Leu Gly Gly Asn
20 25 30
Ile Met Lys Ser Ile Ser Lys Lys Ile Met Ala Gly Leu Gly Val Gly
35 40 45
Ala Met Ser Leu Ser Ile Trp Ala Pro Ser Ser Glu Ala Ala Ala Pro
50 55 60
Ala Lys Asn Glu Tyr His Thr Ile His Leu Ala Ala Asn Thr Asn Leu
65 70 75 80
Ala Trp Asp Val Asp Asn Gly Ser Val Asp Asn Asp Arg Ala Ile Leu
85 90 95
Leu Trp Asn Gly Asp Arg Asn Asp Asn Glu Gln Phe Leu Phe Phe Pro
100 105 110
Leu Asp Gly Gly Ser Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro
115 120 125
Val Thr Phe Gly Gly Pro Thr Trp Ser Gly Ser Gly Gly Ile Leu Gly
130 135 140
Asn Gln Asn Leu Asn Gln Arg Ser Trp Thr Gly Ser Ser Asn Glu Gln
145 150 155 160
Trp Tyr Leu Arg Asp Arg Gly Asn Asn Asn Tyr Glu Ile Val Asn Gln
165 170 175
Gly Asn Gly Lys Val Ala Ser Tyr Ala Tyr Gln Trp Ala Ser Arg His
180 185 190
Ile Glu His Val Asp Leu Asp Glu Ser Asn Pro Ser Asp Asn Asp Arg
195 200 205
Leu Phe His Ile Pro Ala Ala Ala Arg Ser Thr Phe Ser Leu Pro Thr
210 215 220
Leu Pro Ala Val Gly Thr Arg Pro Val Ala Pro Glu Tyr Thr Gln Gly
225 230 235 240
Gly Ser Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val
245 250 255
Gly Ala Ser Leu Val Pro Cys Ile Met Val Lys Asp Ser Gln Ala Ser
260 265 270
Asp Tyr Lys Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu
275 280 285
Glu Tyr Trp Asp Lys Thr Phe Ser Ala Val Ile Pro Ala Gly Leu Thr
290 295 300
Arg Asn Tyr Thr Phe Lys Thr Gly Met Thr Ser Val Asp Gln Gln Lys
305 310 315 320
Met Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Phe Gly Leu Lys
325 330 335
Phe Gly Asp Ala Ser Ala Ser Ile Lys Ser Glu Ile Ser Lys Thr Ile
340 345 350
Gln Thr Glu Ile Ser Thr Thr Asn Thr Glu Ala Thr Glu Glu Thr Ile
355 360 365
Thr Ser Thr Val Thr Ser Glu Leu Gly Lys Thr Thr Gly Phe Thr Glu
370 375 380
Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Val
385 390 395 400
Val Ser Asp Pro Trp Val Val Lys Asn Asn Lys Ile Thr Val Ala Arg
405 410 415
Lys Asn Ser Ile
420
<210> 20
<211> 301
<212> PRT
<213> 芽孢杆菌属的种
<400> 20
Leu Arg Gly Lys Phe Met Lys Lys Leu Met Phe Ser Leu Val Ala Thr
1 5 10 15
Thr Ile Ser Met Gly Leu Ile Leu Gly Ser Ala Pro Ala Lys Ala Asp
20 25 30
Val Ser Asn Lys Asn Ser Ala Tyr Gln Asp Ile Gly Glu Arg Val Lys
35 40 45
Lys Met Ala Gln Ser Ala Ala Trp Gly Gly Gln Glu Tyr Arg Ser His
50 55 60
Asn Ile Lys Asp Ile Glu Leu Lys Gly Asn Leu Ile Asp Gly Ser Met
65 70 75 80
Val Glu Asn Ser Gln Val Leu Thr Val Ser Ser Asp Val Leu Glu Asn
85 90 95
Asn Leu Gly His Thr Val Asn Met Pro Ser Thr Gly Tyr Glu His Glu
100 105 110
Phe Glu Glu Thr Thr Ser Thr Thr Asn Thr Ser Gly Trp Lys Phe Gly
115 120 125
Tyr Asn Tyr Asn Ala Ser Phe Ser Val Leu Met Val Ser Ala Ser Gln
130 135 140
Ser Phe Ser Val Glu Tyr Asp Met Ser Thr Ser Asp Thr His Glu Thr
145 150 155 160
Lys Glu Lys Arg Lys Phe Thr Val Pro Ser Ile Glu Val Pro Val Pro
165 170 175
Ala Gly Lys Lys Tyr Lys Val Glu Tyr Val Phe Glu Lys Val Lys Val
180 185 190
Ser Gly Lys Asn Lys Ile Asp Ala Ser Leu Phe Gly Asp Val Thr Tyr
195 200 205
Tyr Tyr Asn Asn Gln Pro Met Ser Pro Gln Leu Leu Tyr Ser Val Gln
210 215 220
Gly Leu Ala Thr Asp Lys Gln Gly Phe Glu Gln Val Ile Arg Asp Ser
225 230 235 240
Ala Ile Gly Asn Asp Arg Phe Gly Ile Lys Thr Thr Gly Ile Gly Gln
245 250 255
Phe Ser Thr Glu Phe Gly Thr Arg Leu Thr Arg Thr Leu Thr Asp Ile
260 265 270
Thr Asp Ala Arg Asn Pro Val Lys Leu Glu Thr Lys Asn Ile Pro Val
275 280 285
Glu Phe Lys Thr Leu Ser Ile Asp Thr Arg Val Ile Lys
290 295 300
<210> 21
<211> 585
<212> PRT
<213> 芽孢杆菌属的种
<400> 21
Met Lys Pro Lys Ser Glu Glu Thr Gln Pro Pro Ser Pro Lys Trp Ser
1 5 10 15
Lys Ser Glu Tyr Asn Asn Tyr Arg Asn Gly Tyr Ala Tyr Asn Ala Asn
20 25 30
Ile Gly Tyr Glu Asn Lys Asn Trp Met Asn Ala Leu Arg Gly Glu Thr
35 40 45
Lys Ile Ser Gln Leu Ser Ile Pro Gly Thr His Gly Ser Thr Ala Leu
50 55 60
His Gly Ile Pro Gly Ala Asp Arg Trp Ile Ile Asn Gln Arg Met Ser
65 70 75 80
Ile Thr Thr Gln Leu Asn Ser Gly Ile Arg Tyr Leu Asp Ile Arg Ala
85 90 95
Arg Arg Thr Gly Thr Ser Phe Ala Met His His Gly Gln Ala Tyr Gln
100 105 110
Lys Lys Met Phe Gly Asp Ile Leu Asn Glu Val Thr Ala Phe Leu Arg
115 120 125
Gln His Pro Gly Glu Thr Val Leu Met Arg Leu Lys Glu Glu His Thr
130 135 140
Pro Glu Asp Gly Ser Arg Ser Phe Glu Glu Ile Leu Lys Gly Tyr Trp
145 150 155 160
Asp Asn Pro Ser Tyr Lys Asn Tyr Phe Trp Gln Pro Ala Gly Ser Gly
165 170 175
Asn Gly Ser Gly Val Asn Ala Asn Pro Glu Leu Lys Asp Val Arg Gly
180 185 190
Lys Ile Val Leu Leu Gln Gln Phe Ala Val Ser Lys Ala Tyr Gly Ile
195 200 205
Asn Tyr Trp Asn Leu Glu Lys Gln Asp His Asp Asn Val Arg Phe Ser
210 215 220
Met Asp Gly Met Tyr Ala Lys Trp Thr Ala Val Lys Asn His Leu Ile
225 230 235 240
Lys Ala Asn Ser Asp Gln Asn Lys Ile Tyr Leu Asn His Leu Ser Gly
245 250 255
Asn Gly Gly Pro Ser Gly Gly Ala Ala Pro Glu Phe Val Ala Ser Ala
260 265 270
Tyr Gly Gly Asp Gly Glu Pro Leu Met Gly Thr Gly Ser Pro Ser Gln
275 280 285
Tyr Pro Asp Phe Pro Thr Glu Asn Tyr Arg Leu Tyr Phe Gly Gly Met
290 295 300
Asn Ile Met Thr Asn Gln Tyr Ile Pro Arg Ser Gly Leu Gln Tyr Thr
305 310 315 320
Gly Ile Val Ala Ala Asp Phe Pro Gly Lys Gly Leu Val Glu Arg Val
325 330 335
Ile Lys Leu Asn Asp Lys His Ile Asp Val Ser Ala Ile His His Met
340 345 350
Gly Leu Asn Ala Gln Lys Lys Val Glu Ile Gly Phe Thr Gly Asn Tyr
355 360 365
Leu Ser Asn Asn Tyr Val Ile Thr Lys Asn Gly Glu Tyr Ile Ala His
370 375 380
Tyr Asp Lys Gly Thr Ala Tyr Tyr Ala Tyr Leu Ser Lys Thr Asp Tyr
385 390 395 400
Gly His Leu Leu Thr His Asp Asp Gly Tyr Ser Phe Phe Asn Gly Asp
405 410 415
Lys Ile Ala Val Ser Leu Glu His Ser Asn Gly Ala Arg Thr Leu Leu
420 425 430
Lys Glu Phe Thr Val Asn Ile Asp Glu Asn Asn Leu Glu Glu Val Lys
435 440 445
Ile Pro Asp Gly Lys Tyr Asn Ile Val Ser Ala Leu Asn Thr Ser Lys
450 455 460
Val Ala Asp Met Thr Gly Asn Asn Asn Val Val Leu Tyr Thr Lys His
465 470 475 480
Gly Asp Lys Asn Gln Gln Trp Lys Phe Val Tyr Asp Ala Asn Lys Gln
485 490 495
Ser Tyr Gln Ile Arg Ser Glu Trp Asn Thr Asn Val Ala Leu Ser Tyr
500 505 510
Val Ser Gly Ser Gly Asn Val Leu Gly Glu Ser Ile Asn Thr Thr Lys
515 520 525
Glu Glu Gln Tyr Trp Lys Leu Arg Arg Ala Gly Asn Gly Tyr Leu Tyr
530 535 540
Val Glu Asn Lys Lys Asn Gly Trp Val Leu Asp Val Glu Arg Ser Ser
545 550 555 560
Thr Ser Asp Gly Thr Asn Ile Ile Leu Phe Val Arg Thr Gly Thr Thr
565 570 575
Asn Gln Lys Phe Lys Leu Ala Arg Ser
580 585
<210> 22
<211> 348
<212> PRT
<213> 芽孢杆菌属的种
<400> 22
Met Lys Thr Lys Ile Lys Leu Pro Asn Lys Lys Leu Met Ala Lys Ser
1 5 10 15
Ser Ile Ile Ala Leu Ala Thr Ser Phe Ala Leu Ile Ser Pro Asp Leu
20 25 30
Val Asn Ala Asp Val Ile Asn Leu Lys Lys Gly Phe Glu Gln Gln Lys
35 40 45
Gln Ile Ala Ala Asp Val Asp Pro Ile Pro Val Pro Ile Glu Asn Val
50 55 60
Lys Gly Leu Gln Asp Leu Trp Leu Asp Ser Lys Glu Met Lys Cys Pro
65 70 75 80
Leu Ala Asp Leu Ile Gly Thr Ala Pro His Asn Leu Ser Asn Leu His
85 90 95
Phe Asn Phe Leu Tyr Tyr Lys Glu Pro Ser Tyr Ser Val Asp Val Ile
100 105 110
Lys Lys Asn Glu Glu Lys Pro Ser Ser Pro Val Tyr Met Gly Thr Thr
115 120 125
Asn Leu Ser His Ser Gly Gly Thr Ser Glu Gln Thr Leu Tyr Ser Gln
130 135 140
Ala Phe Ser Lys Thr Val Thr Ser Ser Val Thr Thr Thr Ile Thr His
145 150 155 160
Gly Ala Lys Val Gly Ala Lys Ala Gly Ala Lys Leu Gln Val Pro Leu
165 170 175
Val Gly Glu Thr Ser Val Glu Leu Asn Ala Glu Tyr Asn Phe Ala Lys
180 185 190
Ala Gly Ser Glu Thr Gly Thr Glu Asn Val Thr Tyr Thr Val Pro Ser
195 200 205
Gln Pro Val Lys Leu Gln Pro Gly Glu Ser Ala Lys Ile Thr Ala Asn
210 215 220
Leu Arg Met Ala Lys Ala Ser Gly Glu Val Arg Leu Arg Thr Thr Tyr
225 230 235 240
Thr Gly Glu Ile Gln Ser Asn Phe Phe Val Asn Thr Pro Tyr Gly Pro
245 250 255
Met Glu Asp Ser Asp Thr Thr Gly Leu Gly Glu Trp Met Lys Thr Leu
260 265 270
Val Asp Phe Asn Glu Asp Leu Lys Asn Gln Trp Thr Phe Ser Pro Thr
275 280 285
Tyr Ala Ser Val Ala Tyr Gln Asp Gly Lys Gly Thr Tyr Asn Ala Thr
290 295 300
Tyr Gly Ala Val Leu Asp Ile His Val Asp Ile Phe Gly Gly Ser Asn
305 310 315 320
Lys Gly Arg Lys Lys Arg Asp Ala Asp Ser Thr Pro Ile Arg Ser Tyr
325 330 335
Ser Tyr Glu Val Thr Pro Glu Ile Val Glu Asn Asn
340 345
<210> 23
<211> 291
<212> PRT
<213> 芽孢杆菌属的种
<400> 23
Met Ala Ile Ala Asp Leu Asp Ala Tyr Leu Ile Ala Leu Gly Lys Lys
1 5 10 15
Ile Gly Phe Pro Ser Ile Thr Gly Val Asn Thr Ser Asn Leu Arg Val
20 25 30
Glu Asn Ala Glu Ser Tyr Gly Phe Glu Ile Lys Asp Ser Lys Pro Gln
35 40 45
Gly Thr Met Phe Met Gly Glu Ser Glu Leu Lys Asn Asp Thr Asp Gln
50 55 60
Thr Gln Thr Ile Lys Ser Asp Ser Phe Thr Lys Thr Ile Thr Asp Ser
65 70 75 80
Val Thr Leu Ser Val Thr Asn Gly Val Thr Thr Gly Val Ser Val Thr
85 90 95
Ile Ser Gly Lys Ile Phe Gly Ile Gly Ala Glu Ser Ser Met Ser Phe
100 105 110
Glu Val Ser Thr Ser Thr Thr Asn Glu Gln Thr Ser Glu Glu Ser Val
115 120 125
Ala Tyr Thr Val Pro Ser Gln Asn Val Leu Val Pro Ser Arg Lys Thr
130 135 140
Tyr Tyr Val Tyr Thr Ser Leu Gln Arg Ser Gln Leu Glu Gly Thr Ile
145 150 155 160
Arg Leu Lys Ala Asp Leu Ser Asn His Phe Ser Phe Lys Thr Ser Gly
165 170 175
Asn Val Ser Pro Ala Leu Met Asn Ile Tyr Asp Phe Val Asn His Tyr
180 185 190
Gln Ser Leu His Pro Leu Pro Ser Gly Ile Ser Leu Asn His Lys Asp
195 200 205
Lys Ser Ile His Phe Glu Gly Val Ala Glu Tyr Ile Tyr Gly Thr Gly
210 215 220
Thr Lys Phe Tyr Val Thr Ile Ser Asp Lys Pro Ser Ser Gln Gly Thr
225 230 235 240
Gln Glu His Lys Ala Phe Asp Val Lys Thr Gly Leu Gly Thr Tyr Ala
245 250 255
Ile Arg Leu Asp Gly Lys Arg Phe Asp Leu Thr Leu Asn Asp Leu Lys
260 265 270
Asp His Leu Glu Pro Lys Asp Phe Glu Lys Ile Met Glu Leu Gln Asn
275 280 285
Glu Ile Val
290
<210> 24
<211> 288
<212> PRT
<213> 芽孢杆菌属的种
<400> 24
Met Ser Ile Tyr Asn Val Glu Ala Asp Leu Phe Asp Leu Cys Arg Glu
1 5 10 15
Tyr Ala Ile Lys His Tyr Asp Ala Asn Asp Ala Thr Phe His Gly Ala
20 25 30
Thr Ile Tyr Asp Arg Ser Val Leu Asn Ser Glu Val Met Pro Asp Gln
35 40 45
Val Glu Phe Asn Val Ile Pro Asp Leu Ser Asn Thr Val Ile Asn Thr
50 55 60
Ile Thr Asn Ala Thr Ser Ile Glu Gln Ser Met Ser Thr Lys Leu Ser
65 70 75 80
Thr Lys Thr Ile Glu Thr Thr Ser Phe Thr Thr Thr Glu Gly Tyr Lys
85 90 95
Val Gly Thr Ser Ile Lys Asn Thr Leu Glu Phe Lys Ala Gly Gly Leu
100 105 110
Phe Ser Ile Gly Gly Val Glu His Lys Phe Glu Val Ser Val Ser Gly
115 120 125
Glu Tyr Asn His Ser Ser Ser Glu Thr Thr Thr Asn Thr Thr Glu Lys
130 135 140
Leu Trp Glu Tyr Thr Ala Pro Ile Lys Val Pro Pro Lys Thr Lys Val
145 150 155 160
Glu Gly Lys Ile Gln Ile Tyr Ser Gly Ala Ile Pro Val Pro Val Thr
165 170 175
Leu Lys Ser Thr Val Leu Gly Leu Gly Gln Ser Pro Asn Gly Lys Leu
180 185 190
Gln Asn Ala Leu Thr Ser Leu Trp Tyr Lys Asp Lys Ala Gly Lys Glu
195 200 205
Trp Val Asp Thr Tyr Gln Thr Ser His Leu Tyr Thr Leu Arg Glu Glu
210 215 220
Trp Ser Gly Tyr Lys Pro Ile Tyr Val Gly Ser Asp Gly Asn Gly Val
225 230 235 240
Lys Leu Gln Gly Lys Gly Thr Val Gly Leu Asp Leu Gly Leu Tyr Ala
245 250 255
Val Val Thr Phe Lys Glu Glu Pro Leu Pro Gly Asn Tyr Ser Met Gly
260 265 270
Asn Glu Arg Thr Tyr Ser Lys Val Ile Phe Arg Asp Gly Arg Val Ile
275 280 285
<210> 25
<211> 332
<212> PRT
<213> 芽孢杆菌属的种
<400> 25
Met Lys Arg Lys Asn Asn Arg Asn Lys Ser Leu Leu Ile Val Ala Thr
1 5 10 15
Ile Ser Thr Ile Gly Leu Thr Gly Ile Ser Ser Thr Pro Ala Phe Ala
20 25 30
Ala Glu Thr Pro Ala Ile Asn Ser Lys Ala Lys Glu Ser Tyr Tyr Ser
35 40 45
Gly Thr Ile Asp Leu Met Phe Ile Asn Glu Phe Asn Lys Asn Cys Leu
50 55 60
Ser Ile Asp Asn Asn Asn Ser Arg Thr Ile Ile Lys Asp Leu Lys Pro
65 70 75 80
Ala Arg Glu Gln Thr Glu Ser Ile Asp Ser Phe Glu Val Asp Asn Ser
85 90 95
Asn Ser Asn Ser Thr Arg Ile Phe Lys Ser Gln Gln Lys Thr Tyr Lys
100 105 110
Asn Thr Asp Thr Val Thr Val Thr His Ala Lys Glu Phe Ser Phe Gly
115 120 125
Gln Glu Ile Ser Ala Glu Leu Gly Ala Pro Lys Leu Leu Glu Asn Leu
130 135 140
Leu Asn Met Ala Lys Ile Asn Leu Lys Ala Thr Phe Gly Gln Lys Ile
145 150 155 160
Thr Asn Ser Lys Ala Glu Ala Lys Ser Gln Glu Gln Ser Thr Thr Tyr
165 170 175
Gly Gly Asp Asn Ile Glu Val Lys Pro Gly Gln Lys Val Lys Val Glu
180 185 190
Tyr Glu Tyr Ile Thr Asn Ile Tyr Ser Gly Thr Leu Glu Thr Leu Ala
195 200 205
Pro Leu Lys Tyr Asn Val Lys Asp Pro Ser Met Ser Tyr Val Ser Pro
210 215 220
Lys Asp Ile Pro Tyr Tyr Lys Lys Thr Leu Pro Tyr Val Ala Lys Leu
225 230 235 240
Ser Gly Gly Lys Lys Tyr Ser Thr Asp Tyr Glu Phe Phe Lys Asp Val
245 250 255
His Asn Thr Pro Asp Asp Trp Ala Val Leu Ile Ala Tyr Asp Lys Gly
260 265 270
Asp Ala Tyr Phe Phe Tyr Ala Trp Glu Leu Lys Asp Thr Thr Thr Leu
275 280 285
Asn Asp Asn Lys Lys Gln Val Tyr Ser Lys Gly Ser Ala Lys Phe Glu
290 295 300
Ala Val Arg Gly Thr Gly Val Lys Val Lys Val Thr Asp Leu Asp Thr
305 310 315 320
Gly Lys Ile Val Lys Asp Lys Val Val Ser Tyr Phe
325 330
<210> 26
<211> 595
<212> PRT
<213> 芽孢杆菌属的种
<400> 26
Met Lys Tyr Lys Asp Arg Lys Asp Ala Lys Arg Lys Tyr Lys Gln Ala
1 5 10 15
Leu Leu Ala Thr Val Ala Thr Met Thr Leu Gly Val Ser Thr Leu Gly
20 25 30
Ser Thr Ala Ser Ala Phe Ala Glu Glu Lys Asp Lys Asn Ile Ala Gln
35 40 45
Gln Gly Ala Glu Gln Gly Glu Pro Ala Glu Lys Asp Thr Thr Ala Thr
50 55 60
Gln Gln Gln Asn Thr Val Gln Gln Lys Asn Pro Gly Thr Tyr Tyr Glu
65 70 75 80
Asp Glu Lys Leu Asn Pro Ile Ala Gln Leu Asp Lys Trp Thr Gln Asp
85 90 95
Leu Gly Lys Asn Thr Gly Ala Ala Asn Tyr Lys Thr Ser Leu Ser Ala
100 105 110
Val Glu Lys Ile Met Pro Leu Leu Ile Lys Asp Ile Thr Lys Asp Asn
115 120 125
Tyr Asn Asn Ser Leu Arg Ala Val Thr Met Phe Gly Ala Ala Leu Ile
130 135 140
Pro Tyr Gly Gly Ser Phe Ile Ser Pro Leu Ile Gly Leu Ile Trp Pro
145 150 155 160
Glu Ser Lys Asp Asp Ala Ala Leu Lys Leu Lys Lys Gln Leu Asp Asp
165 170 175
Leu Arg Lys Glu Leu Ser Asp Leu Met Asp Glu Lys Ile Asp Arg Phe
180 185 190
Asp Gln Glu Gly Ile Gln Ala Ala Ala Asn Val Ile Asn Glu Asn Leu
195 200 205
Lys Lys Leu Glu Asp Gly Ile Ala Gly Lys Thr Pro Gly Ile Phe Tyr
210 215 220
Asp Asn Gln Lys Lys Leu Glu Asp Gly Ile Ala Gly Lys Ser Glu Gly
225 230 235 240
Ser Phe Tyr Asp Ser Gln Ser Gly Phe Thr Val Val Tyr Ser Ile Asn
245 250 255
Ser Ala Phe Glu Leu Leu Leu Ala Asn Ala Ser Lys Pro Ser Phe Lys
260 265 270
Met Gln Glu Leu Pro Leu Tyr Thr Gly Leu Ala Ala Ala His Leu Gln
275 280 285
Phe Leu Glu Phe Val Lys Gln Asn Gly Lys Asn Pro Lys Ile Ala Leu
290 295 300
Thr Asn Glu Asn Tyr Asn Thr Phe Val Asn Lys Ile Ala Glu Lys Thr
305 310 315 320
Pro Val Tyr Thr Gln His Ile Glu Asp Thr Tyr Asn Gln Phe Arg Ser
325 330 335
Lys Ile Glu Gln Lys Met Thr Pro Leu Leu Gln Gly Val Ile Gly Val
340 345 350
Thr Ser Asp Glu Gln Gln Asn Leu Gly Ala Ala Phe Asp Lys Thr Ile
355 360 365
Glu Leu Gln Arg Lys Val Val Gln Ile Thr Gly Gly Asp Arg Leu Gly
370 375 380
Ile Asn Ser Lys Asp Lys Gln Lys Leu Gln Thr Phe Ile Asp Asn Lys
385 390 395 400
Glu Asn Tyr Lys Lys Leu Met Glu Thr Lys Ser Asp Tyr Tyr Asn Ser
405 410 415
Thr Val Gly Leu Phe Ala Val Thr Gln Gly Ile Pro Ser Lys Ile Gln
420 425 430
Lys Pro Glu Thr Thr Pro Thr Trp Gly Pro Leu Lys Thr Leu Gln Gly
435 440 445
Pro Val Ala Asn Gly Thr Tyr Lys Ile Ala Ser Lys Leu Asp Ser Asn
450 455 460
Met Leu Leu Asp Asn Asp His Asn Arg Asn Arg Leu Ala Thr Leu Asn
465 470 475 480
Lys Asp Ile Thr Thr Lys Asp Asn Leu Trp Gly Leu Gly Trp Glu Leu
485 490 495
Arg Tyr Asn Pro Asp Lys Gln Ala Tyr Lys Phe Ile Ser Lys Leu Gly
500 505 510
Ser Asp Glu Leu Ala Thr Asn Gly Gln Asp Asp Thr Val Phe Ala Thr
515 520 525
Thr Asp Arg Asn Asp Asn Asp Leu Arg Tyr Trp Thr Leu Glu Pro Ala
530 535 540
Ala Glu Asp Gly Tyr Phe Phe Ile Lys Asn Lys Ala Ser Gly Arg Val
545 550 555 560
Leu Asp Val Thr Gly Gly Asn Thr Ala Ala Gly Thr Asp Ile Lys Thr
565 570 575
Asn Val Gln Asn Thr Pro Ala Val Ala Ala Gln Leu Phe Lys Leu Glu
580 585 590
Thr Val Gln
595
<210> 27
<211> 303
<212> PRT
<213> 赖氨酸芽孢杆菌属的种(Lysinibacillus sp.)
<400> 27
Met Arg Glu Tyr Phe Met Lys Lys Leu Thr Leu Ala Thr Leu Ala Thr
1 5 10 15
Phe Met Ile Val Gly Leu Ile Phe Gly Ala Val Pro Ala Glu Val Lys
20 25 30
Ala Ser Glu His Gly Lys Asn Asn Val Tyr Gln Asp Leu Asp Glu Arg
35 40 45
Val Lys Arg Met Leu Ile Ser Ala Ala Ala Pro Gly Thr Glu Tyr Arg
50 55 60
Tyr His Asn Ile Ile His Ala Glu Leu Asn Gly Gly Asp Val Glu Gly
65 70 75 80
Thr Leu Ile Glu Asn Ser Glu Thr Leu Thr Val Ser Arg Asn Thr Leu
85 90 95
Glu Asn Asn Phe Asp His Glu Ile Glu Leu Pro Thr Ser Gly Tyr Glu
100 105 110
His Glu Phe Thr Glu Thr Thr Ser Thr Thr Asn Ser Thr Gly Trp Thr
115 120 125
Phe Gly Tyr Asn Tyr Asn Val Glu Ile Ser Ile Pro Met Val Ser Gly
130 135 140
Gly His Ser Phe Ser Val Asp Tyr Asn Met Ser Thr Ala Asn Thr Val
145 150 155 160
Glu Lys Ser Glu Thr Arg Arg Phe Ser Val Pro Ser Val Leu Ile Pro
165 170 175
Val Pro Ala Gly Arg Lys Tyr Leu Val Glu Tyr Arg Phe Glu Lys Gln
180 185 190
Thr Val Ser Gly Arg Asn Arg Ile Asn Ala Asp Leu Phe Gly Asp Ala
195 200 205
Val Tyr Tyr Phe Asn Asn Gln Pro Met Ser Pro Gln Leu Leu Tyr Ser
210 215 220
Ala Leu Arg Phe Ala Thr Asp Thr Gln Gly Phe Glu Gln Val Ile Arg
225 230 235 240
Asp Ser Ala Val Gly Asn Asp Arg Phe Gly Ile Arg Gly Thr Gly Val
245 250 255
Gly Gln Phe Arg Thr Glu Phe Gly Thr Ser Leu Tyr Ile Arg Thr Thr
260 265 270
Asp Ile Thr Asn Ser Gln Glu Pro Val Ile Ile Glu Thr Ile Thr Ile
275 280 285
Pro Val Lys Ile Glu Thr Ile Ser Glu Lys Thr Lys Val Val Glu
290 295 300
<210> 28
<211> 341
<212> PRT
<213> 芽孢杆菌属的种
<400> 28
Met Arg Tyr Lys Tyr Lys Ile Lys His His Asn Lys Lys Lys Gly Ala
1 5 10 15
Ile Asn Met Lys Lys Asn Lys Lys Lys Gln Ile Leu Ser Leu Ala Ile
20 25 30
Leu Thr Ser Ile Gly Ala Ser Met Thr Leu Ala Ser Pro Gly Ser Ala
35 40 45
Ser Ala Asp Gln Ile Ser Pro Ile Gln Lys Ile Thr Gln Glu Gln Gln
50 55 60
Asn Val Ala Ile Ser Asp Trp Arg Glu Pro Phe Gln Lys Thr Tyr Lys
65 70 75 80
Ser Ala Lys Leu Arg Asp Pro Asn Arg Tyr Phe Ser Asn Glu Ala Lys
85 90 95
Ile Asp Leu Trp Gly Val Tyr Gln Ser Ser Val Glu Ala Asp Gly Ser
100 105 110
Pro Thr Ile Thr Asn Ser Lys Ser Leu Phe Val Gly Arg Thr Ile Leu
115 120 125
Thr Asn His Thr Asn Gln Ala Gln Thr Leu Thr Thr Asn Glu Phe Ser
130 135 140
Lys Ser Phe Gly Asn Ser Val Thr Asn Ser Thr Thr Asn Gly Phe Asn
145 150 155 160
Leu Gly Val Ser Thr Ser Ala Ser Phe Gly Ile Pro Leu Ile Gly Glu
165 170 175
Thr Gly Ile Glu Ile Ser Thr Glu Tyr Asn Phe Ser Asn Thr Ala Glu
180 185 190
Lys Thr Lys Thr Glu Ser Tyr Met Tyr Thr Ala Ser Pro Gln Asn Ile
195 200 205
Leu Val Pro Ala Asn Ser Ser Val Glu Val Ile Val Asn Leu Asn Thr
210 215 220
Ala Lys Leu Asn Gly Asn Val Lys Leu Leu Ser His Val Asn Gly Gln
225 230 235 240
Leu Met Trp Asn Thr Ser Lys Thr Gly Gln Lys Ile Ser Thr Ser Phe
245 250 255
Lys Gly Phe Leu Glu Lys Ala Ala Arg Pro Asn Glu Ile Ala Ser Asn
260 265 270
Val Lys Ala His Pro Arg Asn Gly Ala Tyr Leu Ile Gly Lys Gly Lys
275 280 285
Tyr Ser Ala Glu Tyr Gly Thr Glu Phe Ser Val Thr Val Arg Pro Val
290 295 300
Gln Lys Pro Ser Thr Val Ala Ala Lys Ser Lys Ser Met Val Ser Glu
305 310 315 320
His Ser Lys Thr Thr Ser Glu Ser Tyr Thr Tyr Thr Val Lys Pro Glu
325 330 335
Val Lys Lys Glu Gln
340
<210> 29
<211> 380
<212> PRT
<213> 芽孢杆菌属的种
<400> 29
Met Leu Gln Asn Lys Asn Lys Leu Phe Phe Thr Gly Ile Ser Ala Leu
1 5 10 15
Thr Ile Met Thr Ser Gly Leu Thr Ile Ser Thr Pro Thr Ala Ser Ala
20 25 30
Asn Thr Gln Glu Ser Thr Pro Ser Val Tyr Pro Ile Gln Gly Thr Glu
35 40 45
Phe Ser Asp Glu Asn Gly Gln Val Ile Ser Pro Gly Ala Phe Ala Thr
50 55 60
Leu Ser Pro Tyr Trp Leu Ile Asn Asp Ser Asn Asp Gln Leu Leu Asn
65 70 75 80
Phe Tyr Asn Gln Val Lys Gln Arg Leu Leu Asp Lys Gln Asn Thr Glu
85 90 95
Gln Asn Met Leu Lys Leu Asp Glu Tyr Leu Asn Asp Leu Trp Phe Val
100 105 110
Gly Ala Val Ser Glu Leu Tyr Ala Thr Pro Thr Asp Tyr Asn Lys Arg
115 120 125
Leu Glu Ala Ser Gln Leu Val Arg Trp Tyr Lys Asn Asn Asn Ala Ala
130 135 140
Arg Pro Thr Phe Glu Ile Leu Glu Ser Lys Leu Glu Asn Val Glu Pro
145 150 155 160
Ile Leu Asp Ile Thr Glu Pro Ile Gly Tyr Phe Ala Thr Glu Ser Glu
165 170 175
Tyr Thr Asn Asn Ser Pro His Gln Gly Glu Tyr Leu Ile Pro Gly Trp
180 185 190
Ser Asn Thr Thr Ser Thr Thr Tyr Asn Thr Thr Thr Thr Lys Gly Phe
195 200 205
Ser Ile Gly Ala Asn Thr Gly Ile Glu Ile Pro Leu Pro Phe Met Asp
210 215 220
Lys Leu Gly Leu Thr Gly Ser Tyr Asn Phe Ser Lys Ala Glu Asn Val
225 230 235 240
Ser Thr Thr Asp Ser Thr Thr Leu Thr Ile Pro Val Gln Thr Ile Gln
245 250 255
Val Ser Pro Phe Ser His Ser Lys Thr Ser Phe Tyr Phe Gln Thr His
260 265 270
Gln Tyr Arg Ala Lys Phe Asp Val Thr Ser Lys Phe Lys Ala Asp Val
275 280 285
Gly Arg Asp Ala Glu Tyr Thr Lys Lys Arg Ser Ala Phe Tyr Leu Leu
290 295 300
Ala Lys Tyr Asn Glu Tyr Leu Pro Lys Gly Ile Val Val Glu Asn Gly
305 310 315 320
Gly Lys Leu Thr Leu Glu Gly Lys Gly Glu Ile Ser Val Pro Asn His
325 330 335
Ile Asn Gly Phe Lys Leu Val Lys Glu Thr Lys Glu Thr Pro Leu Leu
340 345 350
Gly Gly Glu Gly Lys Thr Thr Lys Glu Ile Tyr Thr Lys Asp Pro Asn
355 360 365
Thr Asn Lys Leu Ile Pro Asp Ala Ser Ser Lys Ser
370 375 380
<210> 30
<211> 257
<212> PRT
<213> 芽孢杆菌属的种
<400> 30
Met Asp Leu Ser His Leu Asn Leu Ser Glu Val Ser Glu Gly Ile Lys
1 5 10 15
Lys Leu Glu Ala Ser Asp Phe Tyr Met Asn Gln Tyr Leu Glu Leu Tyr
20 25 30
Asp Arg Pro Ala His Leu Val Ile Ile Ser Gln Asn Val Ser Glu Ser
35 40 45
Ile Asp Pro Asn Tyr Gln Ser Met Ser Tyr Leu Pro Ala Asn Gly Ala
50 55 60
Asp Asp Thr Ala Phe Asp Leu Leu Lys Lys Phe Asp Pro Ala Val Val
65 70 75 80
Thr Ser Pro Ala Pro Pro Gly Gly Asn Ser Gly Leu Met Met Asp Met
85 90 95
Ala Val Ala Gln Lys Ile Ala Lys Ser Leu Lys Tyr Gly Val Ile Glu
100 105 110
Gly Val Asp Ile Lys Ser Lys Gln Ser Asn Ser Val Lys Ile Ala Asp
115 120 125
His Ile Thr Ala Val Tyr Asn Met Ile Ser Ser Leu Val Thr Thr Val
130 135 140
Ala Ser Asp Ser Val Ser Gly Phe Thr Ser Ile Leu Lys Ala Gly Ile
145 150 155 160
Thr Asn Leu Phe Thr Asp Ile Gly Lys Glu Val Asp Asn Gly Ala Gly
165 170 175
Gly Ile Lys Lys Arg Trp Ala Val Val Thr Tyr Asp Lys Ser Asn Glu
180 185 190
Leu Ser Tyr Thr Tyr Asn Gln Met Phe Ala Tyr Pro Asn Thr Asn Gly
195 200 205
Lys Tyr Ile Gly Met Met Leu Met Ser Thr Gln Thr Lys Ile Thr Ser
210 215 220
Asn Ser Leu Asp Leu Leu Ile Leu Lys Pro Tyr Asp Ile Ala Thr Val
225 230 235 240
Asn Ser Thr Thr Asn Ile Ile Arg Leu Val His Lys Ile Gly Pro Val
245 250 255
Glu
<210> 31
<211> 947
<212> PRT
<213> 芽孢杆菌属的种
<400> 31
Met Ile Met Gln Met Val Thr Lys Ala Met Asn Pro Lys Leu Gly Ala
1 5 10 15
Leu Pro Asp Phe Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Gly
20 25 30
Asn Ile Thr Asp Val Met Ser Thr Ile Phe Gly Val Asp Thr Gly Asp
35 40 45
Ser Ser Ile Asp Asp Val Leu Asn Asn Glu Glu Leu Leu Lys Gln Met
50 55 60
Met Glu Gln Met Asp Thr Met Gln Asp Ser Leu Glu Asp Ile Leu Lys
65 70 75 80
Asn Gln Glu Ile Ser Lys Asn Ile Gln Glu Gln Ile Leu Ala Leu Thr
85 90 95
Thr Asp Leu Ala Thr Ser Ile Asn Lys Glu Leu Val Lys Ile Glu Gly
100 105 110
Ile Leu Thr Thr Tyr Leu Asn Ala Ile Ser Gly Met Leu Lys Asp Ile
115 120 125
Tyr Ser Gln Thr Ser Val Ile Asp Gln Lys Val Asp Lys Leu Leu Ala
130 135 140
Met Met Thr Phe Ala Leu Lys Glu Leu Asp Tyr Ile Lys Asp Asn Val
145 150 155 160
Val Leu Asn Ser Ser Ile Val Glu Ile Thr Pro His Val Gln Lys Leu
165 170 175
Val Tyr Val Asn Lys Lys Phe Leu Ser Leu Thr Arg Gly Phe Leu Gln
180 185 190
Gly Glu Val Leu Ser Ile Asp Ser Met Gln Glu Ile Gln Glu Trp Ala
195 200 205
Lys Ser Ile Leu Ala Thr Glu Met Asn Ser Phe Glu Phe Ser Val Asp
210 215 220
Thr Leu His Asn Ile Ile Ile Gly Asp Asn Leu Tyr Lys Arg Ser Ala
225 230 235 240
Leu Lys Thr Phe Ser Asp Val Leu Leu Asp Asp Ala Asp Gln Tyr Gly
245 250 255
Asp Phe Gly Thr Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser Ser Leu
260 265 270
Ala Thr Leu Gln Ile Asn Ala Tyr Leu Cys Leu Thr Phe Ala Arg Lys
275 280 285
Val Leu Gly Leu Ser Glu Ile Asp Tyr Gln Val Thr Met Gln Asp Arg
290 295 300
Ile Glu Gln Gln Asn Gln Met Phe Val Asn Leu Ile Gln Asp Lys Asn
305 310 315 320
Tyr Ser Asn Ala Leu Glu Thr Lys Gly Ile Tyr Pro Met Pro His Asn
325 330 335
Val Gly Asp Cys Asn Ser Phe Asp Leu Gln Ala Lys Asn Gly Tyr Ala
340 345 350
Leu Ile Gly Leu Glu Phe Phe Met Asp Asn Asn Ile Tyr Lys Ile Lys
355 360 365
Ala Tyr Gln Gly Lys Ile Asp Lys Asn Phe Ser Leu Tyr Ala Asp Thr
370 375 380
Val Glu Glu Ile Ile Ser Asp Asp Leu Ser Ala Val Phe Pro Ala Ile
385 390 395 400
Thr Glu Asn Ala Pro Arg Leu Asp Ile Val Phe Pro Leu Ser Gly Glu
405 410 415
Leu Ile Gly Pro Pro Asn Thr Val Ile Thr Arg Ile Gly Leu Gly Thr
420 425 430
Lys Tyr Asp Lys Asn Arg Glu Ser Asp Val Arg Thr Phe Ala Tyr Ile
435 440 445
Asp Ala Asp Phe Ser Leu Tyr Asp Tyr Ile Ser Gly Thr Ile Ser Lys
450 455 460
Glu Gly Thr Gln Thr Val Ala Leu Glu Gly Asn Ala Gln Asn Gly Cys
465 470 475 480
Arg Cys Tyr Asp Met Trp Pro Ile Gly Leu Ile Gly Asp Leu Tyr Met
485 490 495
Thr Pro Leu Lys Gly Leu Ser Leu Thr Val Asp Glu Gly Thr Leu Asn
500 505 510
Met Ser Gly Glu Ser Tyr Phe Ser Thr Ile Leu Ser Arg Glu Tyr Asn
515 520 525
Thr Asn Phe Ile Leu Phe Pro His Ser Ser Asn Ser Gly Ser Ile Asn
530 535 540
Glu Asn Leu Ile Arg Asn Gly Asp Leu Glu Glu Gln Asp Lys Tyr Trp
545 550 555 560
Glu Gly Thr Glu Pro Ser Phe Phe Val Glu Gly Glu Gly Thr Phe Gly
565 570 575
Ser Asn Ala Leu Lys Val Gln Ala Gln Gly Ala Phe Asp Gln Ile Val
580 585 590
Lys Leu Glu Pro Asn Thr Thr Tyr Arg Leu Thr Ala Phe Thr Lys Val
595 600 605
Lys Asp Ser Asn Tyr Ser Lys Gly Arg Ile Gly Ile Arg Asp Lys Tyr
610 615 620
Ala Tyr Arg Val Glu Glu Ser Phe Leu His Thr Arg Tyr Asn Gln Thr
625 630 635 640
Lys Val Glu Phe Thr Thr Gly Ala Asp Thr Ser Lys Leu Ser Val Phe
645 650 655
Ile Gln Gly Gly Gly Asp Gln Glu Ser Ile Ala Trp Ala Asp Asn Ile
660 665 670
Glu Leu Tyr Lys Val His Gln Gln Asp Asn Met Ile Ser Asp Phe Asn
675 680 685
Phe Asp Thr Tyr Lys Tyr Ser Asn Met Cys Trp Glu Leu Ser Gly Gly
690 695 700
Gly Asp Leu Val Glu Gln Glu Gly Leu Phe Asn Ser Lys Ala Leu Lys
705 710 715 720
Ile Thr Asn Ser Gly Gly Ala Glu Gln Glu Val Lys Leu Lys Ala Asn
725 730 735
Thr Asn Tyr Ile Phe Thr Ala Tyr Val Lys Val Asp Asn Thr Thr Ala
740 745 750
Gln Ile Gly Cys Gly Ser Asn Gln Val Ser Cys Asn Ser Thr Ser Tyr
755 760 765
Thr Pro Val Lys Leu Lys Phe Arg Thr Gly Val Asp Pro Ser Thr Thr
770 775 780
Glu Gly Ser Ile Tyr Cys Ser Asn Leu Asn Asp Ser Gly Thr Val Trp
785 790 795 800
Ala Asp Asn Phe Val Leu Cys Glu Ala Pro Asn Leu Ile Ile Asn Gly
805 810 815
Asp Phe Glu Gln Phe Asp Leu Ser Ser Trp Asn Leu Ser Pro Ser Glu
820 825 830
Gly Gly Ser Ile Tyr Leu Gln Ser Gly Leu Gly Met Ser Arg Ser Asn
835 840 845
Ala Ile Val Leu Arg Gly Thr Gly Asn Glu Ala Gly Lys Ile Ser Gln
850 855 860
Lys Val Pro Leu Lys Pro Phe Thr Lys Tyr Arg Leu Thr Ala Tyr Val
865 870 875 880
Lys Val Ser Lys Gly Ser Ile Ala His Ile Gly Tyr Gly Asp Asn Thr
885 890 895
Cys Ala Cys Ala Val Asp Asp Phe Arg Gln Ala Leu Val Asp Phe Thr
900 905 910
Thr Gly Ala Asn Pro Met Gln Ser Asp Asp Ala Ile Tyr Leu Ser Ser
915 920 925
Gly Asn Ser Lys Tyr Thr Val Ala Asp Asn Phe Glu Leu Tyr Glu Leu
930 935 940
Asp Gln Ile
945
<210> 32
<211> 426
<212> PRT
<213> 芽孢杆菌属的种
<400> 32
Met Asn Leu Pro Arg Leu Glu Lys Asn Gly Leu Arg Lys Leu Glu Lys
1 5 10 15
Asp Val Ile Ser Leu Leu Glu Val Asn Leu Asp Asn His Pro Leu Tyr
20 25 30
Phe Lys Asn Tyr Glu Val Ile Pro Glu Glu Val Lys Ala Phe Tyr Pro
35 40 45
Leu Tyr Leu Ala Ser Ala Asp Glu Asp Thr Gly Lys Tyr Gly Pro Gly
50 55 60
Asp Leu Ser Thr Pro Asp Gly Phe Ile Ile Ser Arg Glu Asp Trp Ser
65 70 75 80
Tyr Val Gln Ser Tyr Ile Gln Thr Gly Met Gly Leu Pro Thr Asp Ala
85 90 95
Asp Ala Met Arg Glu His Leu Thr Ile Pro Ala Ser Thr Ala Met Pro
100 105 110
Asp Asp Phe Asn Arg Val Tyr Asn Val Tyr Ser Ser Asp Lys Phe Leu
115 120 125
Cys Asp Trp Trp Asn Lys Thr Leu Trp Pro Ser Ile Phe Lys Ala Ala
130 135 140
Ser Leu Val Thr Asn Tyr Gly Asn Val Val Asp Gly Ile Val Asp Lys
145 150 155 160
Asn Thr Gly Lys Gly Gln Asp Ser Ser Ser Tyr Tyr Ala Gln Ile Ile
165 170 175
Lys Tyr Leu Glu Gln Leu Lys Thr Ala Asn Glu Ser Gln Arg Glu Thr
180 185 190
Ile Val Lys Asn Ile Gln Ser Tyr Cys Leu Thr Leu Lys Lys Thr Ala
195 200 205
Leu Lys Ala Val Thr Gln Ala Asn Ser Val Thr Thr Gln Leu Asn Ala
210 215 220
Phe Ile Asn Gly Asp Ala Thr Gln Lys Ile Asp Gly Val Leu Val Ile
225 230 235 240
Gln Asn Arg Leu Lys Asp Val Gln Thr Phe Leu Asn Gln Asn Tyr Gly
245 250 255
Pro Asn Ser Gln Asp Gln Gln Asp Leu Asn Asn Gln Ile Thr Asp Leu
260 265 270
Lys Lys Gln Ser Asp Asp Leu Gln Asn Ser Lys Asn Lys Leu Lys Gln
275 280 285
Lys Leu Lys Phe Leu Tyr Phe Gly Ala Leu Ile Gly Phe Leu Val Tyr
290 295 300
Glu Ile Ala Thr Gly Val Glu Gly Lys Lys Ile Gln Lys Gln Ile Asp
305 310 315 320
Asp Ile Gln Asn Gln Ile Asn Gly Lys Gln Ala Glu Leu Gln Arg Asp
325 330 335
Thr Asn Ile Thr Asn Met Ala Asn Ser Ile Asn Gln Asp Ile Gly Gln
340 345 350
Met Leu Asp Gln Gly Gln Asn Ala Ile Pro Val Phe Val Lys Ile Gln
355 360 365
Asn Asn Trp Gln Thr Ile Gly Gly Gln Met Asp Glu Leu Ala Glu Ser
370 375 380
Val Ala Met Leu Gln Asn Ala Ala Asp Ile Asn Ile Val Ile Leu Gln
385 390 395 400
Leu Asn Gln Ala Arg Gln Leu Trp Ala Gln Ile Arg Asp Glu Ala Ile
405 410 415
Gly Phe Thr Met Asn Thr Gln Ala Ser Ser
420 425
<210> 33
<211> 326
<212> PRT
<213> 芽孢杆菌属的种
<400> 33
Met Gln Lys Asn Lys Arg Ile Ile Arg Gly Ile Leu Thr Leu Ser Ile
1 5 10 15
Ile Ala Pro Ile Ser Thr Ser Phe Leu Leu Thr Pro Leu Ser Ser Val
20 25 30
Asn Ala Asp Gln Val Asn Pro Thr Glu Lys Asn Ile Arg Val Lys Thr
35 40 45
Asn Thr Ser Met Gly Ile Ile Lys Trp Glu Asp Leu Ile Phe Asp Asn
50 55 60
Leu His Val Ile Gly Lys Asn Ser Gly Leu Pro Gly Lys Tyr Gln Asp
65 70 75 80
Thr Ile Ser Leu Ser Ser Gly Tyr Phe Trp Asp Val Glu Tyr His Gln
85 90 95
Phe Ser Val Glu Ala Asp Gly Ser Pro Ile Met Thr Asp Ser Asn Asp
100 105 110
Thr Phe Val Ala Lys Thr Thr Leu Thr Asn Asp Thr Asp Gln Glu Gln
115 120 125
Thr Leu Ser Thr Asn Ala Phe Ser Lys Thr Ile Thr Asn Ser Val Ser
130 135 140
Asn Thr Thr Thr His Gly Phe Lys Leu Gly Ala Lys Ala Thr Ala Lys
145 150 155 160
Leu Ser Val Pro Leu Ile Gly Glu Val Gly Thr Glu Leu Ser Thr Glu
165 170 175
Tyr Asn Phe Ser Asp Thr Leu Ala Arg Ser Asn Ser Gly Ser Tyr Thr
180 185 190
Tyr Ile Gly Ser Pro Gln Asn Ile Lys Val Pro Ala His Ser Ser Leu
195 200 205
Glu Val Ile Val Lys Leu Glu Thr Val Thr Ala Lys Gly Asn Val Arg
210 215 220
Leu Leu Thr Lys Ile Ser Gly Arg Asp Arg Gly Asn Phe Ile Tyr Thr
225 230 235 240
Glu Pro Glu Leu Ala Asp His Phe Asp Leu Ser Phe Asn Gln Ile Val
245 250 255
Glu Lys Ala Ser Gln Tyr Lys Asp Phe Lys Asn Ile Val Ala Asn Pro
260 265 270
Asp Gln Glu Thr Ile Thr Leu Ile Gly Thr Gly Glu Tyr Glu Ser Lys
275 280 285
Tyr Gly Thr Glu Phe Ser Val Thr Val Lys Pro Ile Tyr Thr Asn Asn
290 295 300
Arg Glu Lys Arg Glu Ile Asn Glu Gly Tyr Thr Tyr Lys Val Lys Pro
305 310 315 320
Gln Ile Ile Lys His Asp
325
<210> 34
<211> 596
<212> PRT
<213> 芽孢杆菌属的种
<400> 34
Met Lys Tyr Asn Asp Arg Lys Asp Ala Lys Arg Lys Tyr Lys Gln Ala
1 5 10 15
Phe Leu Ala Thr Val Ala Thr Met Thr Leu Gly Val Ser Thr Leu Gly
20 25 30
Ser Thr Ala Ser Ala Phe Ala Glu Glu Lys Lys Thr Glu Val Gln Pro
35 40 45
Gln Gln Ala Thr Pro Asn Ser Asn Ser Leu Gly Ala Gly Gly Gly Ser
50 55 60
Tyr Glu Ser Asp Ala Leu Lys Ala Leu Thr Asp Lys Ile Asn Met Asp
65 70 75 80
Tyr Leu Lys Lys Ala Gly Ser Val Ala Ala Asp Thr Leu Lys Ala Ala
85 90 95
Tyr Lys Asp Ala His Thr Ser Asn Gly Asn Tyr Asn Asn Thr Phe Arg
100 105 110
Thr Leu Thr Met Thr Thr Ser Ala Leu Ile Pro Tyr Gly Gly Val Leu
115 120 125
Ile Ser Pro Ile Ile Gly Leu Ile Trp Pro Glu Asp Leu Thr Ala Glu
130 135 140
Asn Asn Lys Met Ala Ala Leu Ile Lys Gln Leu Asp Glu Met Met Asp
145 150 155 160
Thr Lys Val Thr Asp His Val Val Arg Arg Leu Lys Thr Glu Val Asn
165 170 175
Asp Val Lys Lys Gln Leu Glu Leu Phe Glu Gln Asn Val Asn Thr Gly
180 185 190
Lys His Lys Ser Gly Ser Gly Leu Tyr Ser Leu Thr Glu Glu Asp Gly
195 200 205
Asn Ala Ile Asp Ala Lys Asp Ile His Lys Ala Phe Asn Ser Leu Leu
210 215 220
Glu His Ser Lys Glu Glu Met Gln Glu Val Ala Ala Leu Pro Ile Tyr
225 230 235 240
Thr Ala Met Ala Thr Ala His Leu Glu Phe Leu His Phe Leu Glu Gln
245 250 255
Asn Gly Thr Lys Pro Ile Ile Gly Phe Thr Ser Asn Ala Leu Asn Asn
260 265 270
Ile Leu Lys Thr Arg Glu Gly Leu Glu Asn Glu Thr Phe Gly Gln Lys
275 280 285
Ile Lys Asp Leu Ala Asp Lys Tyr Thr Lys His Val Gln Glu Thr Tyr
290 295 300
Asn Ile Gly Arg Asp Lys Tyr Met Ala Lys Met Gln Ala Ile Thr Thr
305 310 315 320
His Ser Met Asn Glu Asn Asp Glu Tyr Ala Asn Leu Ala Tyr Leu Lys
325 330 335
Ser Leu Asp Asp Ala Arg Ser Glu Met Gly Tyr Ser Gln Ser Lys Ile
340 345 350
Asp Lys Ile Lys Asn Asp Ile Lys Asn Tyr Gln Asn Leu Ile Lys Glu
355 360 365
Lys Ala Asp Phe Arg Asp Gln Thr Ala Glu Asn Val Ala Phe Lys Leu
370 375 380
Thr Ala Met Gly Lys Trp Asn His Gln Gly Asp Lys Trp Tyr Tyr Ile
385 390 395 400
Asn Asn Gln Gly Glu Asn Gln Thr Gly Trp Leu Gln Asp Gly Ser Lys
405 410 415
Tyr Tyr Tyr Phe Ser Pro Glu Lys Thr Asp Lys Phe Glu Lys Gly Gln
420 425 430
Met Val Thr Gly Lys Val Thr Ile Asp Lys Gly Glu Thr Tyr Tyr Phe
435 440 445
Asn Pro His Gly Gly Gln Met Gln Thr Gly Trp Ile Gln Asp Asp Gly
450 455 460
Lys Gln Tyr Tyr Phe Ser Pro Ala Asn Asn Thr Lys Asn Tyr Asp Ser
465 470 475 480
Glu Ser Phe Lys Glu Gly Gln Met Met Thr Gly Trp Leu Ala His Leu
485 490 495
Asp His Tyr Tyr Tyr Met Ser Pro Gly Asp Asp Lys Thr Asn Pro Tyr
500 505 510
Gln Lys Gly Lys Thr Phe Lys Lys Gly Glu Met Trp Thr Gly Trp Leu
515 520 525
Glu Asn Pro Lys Lys Ala Asn Glu Trp Tyr Tyr Met Thr Pro Val Ala
530 535 540
Arg Asp Tyr Lys Val Pro His Leu Ile Gly Asn Asp Asp His Ile Ile
545 550 555 560
Arg Pro Val Gly Ser Met Leu His Asp Glu Tyr Leu Glu Lys Gln Asp
565 570 575
Ser Gly Asp Gly Trp Arg Tyr Tyr Phe Asn Glu Ser Gly Ile Asn Thr
580 585 590
His Arg Tyr Asn
595
<210> 35
<211> 331
<212> PRT
<213> 芽孢杆菌属的种
<400> 35
Val Lys Lys Tyr Lys Lys Leu Ala Val Val Met Pro Leu Ala Ser Leu
1 5 10 15
Leu Thr Thr Gly Ile Gly Val Leu Glu Arg Pro Ser Ser Ser Phe Ala
20 25 30
Asp Thr Ile Pro Gln Lys Lys Leu Ile Gln Met Gln Thr Thr Tyr Leu
35 40 45
Met Thr Asp Gln Gln Ala Lys Asp Gln Ala Lys Gln Glu Val Ala Gln
50 55 60
Val Asn Lys Asp Leu Ser Ser Leu Val Gly Tyr Ser Val Asp His Ala
65 70 75 80
Ser Phe Gln Leu Ile Ser Thr Asn Ala Asn Thr Ile Glu Leu Thr Ser
85 90 95
Ala Glu Ala Thr Gly Asp Ser Asn Ile Glu Leu Gly Asn Tyr His Asn
100 105 110
Gly Thr Asp Leu Ile Gln Asp Tyr Lys Val Pro Gly Lys Lys Glu Thr
115 120 125
Asn Thr Ser Ser Ala Thr Ile Thr Asn Asn Glu Asp Ala Ser Ile Gly
130 135 140
Phe Glu Ser Asp Thr Glu Phe Glu Ile Gly Ile Pro Leu Phe Ala Lys
145 150 155 160
Gly Lys Glu Thr Leu Lys Phe Thr Thr Asn Phe Lys Tyr Ser His Gly
165 170 175
Asp Ser Arg Thr Ser Thr Thr Thr Glu Glu Val Thr Tyr Pro Glu Gln
180 185 190
Val Leu Lys Cys Ser Pro His Gly Thr Thr Lys Leu Val Ser Lys Val
195 200 205
Gln Lys Thr Asn Phe Thr Gly Lys Tyr Ser Gly Lys Ala Leu Ile Thr
210 215 220
Gly Val Lys Asp Ala Asp Gly Lys Ser Leu Asn Ser Glu Glu Leu Phe
225 230 235 240
Thr Leu Tyr Thr Leu Asn Leu Ala Val Asp Ala Ala Ala His Phe Gly
245 250 255
Asp Pro Asn Tyr Glu Gly Met Lys Ile Pro Asp Ser Leu Glu Val Asp
260 265 270
Trp Gln Asn His Lys Ile Tyr Ala Lys Ser Ile Thr Thr Thr Phe Gln
275 280 285
Gly Val Ala Gly His Ser Ser Asp Ala Asn Val Glu Phe Ile Pro Asp
290 295 300
Asp Lys Ala Asn Gln Ser Val Thr Met Ala Leu Ser Glu Tyr Asn Arg
305 310 315 320
Arg Ile Ala Ala Gly Ile Ser Leu Tyr Thr Lys
325 330
<210> 36
<211> 350
<212> PRT
<213> 未知
<220>
<223> 来自环境样品的生物体
<400> 36
Leu Val Gly Asn Thr Gln Gln Asp Leu Cys Ile Thr Ser Leu Tyr Asn
1 5 10 15
Lys Asn Ile Asp Gly Ser Lys Asn Ile Lys Lys Gly Gly Lys Phe Met
20 25 30
Thr Ile Val Asp Ile Glu Gln Lys Ala Tyr Glu Glu Leu Lys Trp Tyr
35 40 45
Ala Ile Arg Glu Leu Asn Ala Gln Ala Asn Ser Phe Thr Asn Pro Ser
50 55 60
Leu Tyr Ser Tyr Asn Ile Asn Asn Ala Asp Ala Val Pro Asp Ile Val
65 70 75 80
Asn Phe Lys Ser Asn Pro Gln Ile Leu Leu Thr Ser Val Gln Val Leu
85 90 95
Ser Asn Asp Thr Ser Ile Gln Gln Ser Gln Thr Leu Lys Phe Ser Gln
100 105 110
Lys Cys Thr Glu Thr Thr Thr Thr Ser Thr Thr Thr Gly Tyr Lys Ile
115 120 125
Gly Thr Ser Ile Lys Ser Thr Thr Lys Gly Lys Ile Lys Val Gly Phe
130 135 140
Leu Met Ala Gly Glu Ile Glu Gln Thr Val Glu Val Ser Leu Thr Gly
145 150 155 160
Glu Tyr Asn His Ser Thr Thr Gln Thr Thr Thr Ser Ser His Glu Glu
165 170 175
Leu Trp Glu Tyr Thr Gln Pro Val Leu Val Pro Ala Arg Ser Lys Val
180 185 190
Ile Ala Thr Met Tyr Ile Met Gly Gly Pro Val Thr Val Pro Met Thr
195 200 205
Leu Ser Ala Asn Ile Ala Gly Thr Gly Val Ser Pro Lys Gly Ser Pro
210 215 220
Tyr Ser Leu Ser Lys Ile Tyr Phe Lys Lys Gln Asn Ser Ser Ala Asn
225 230 235 240
Ser Ser Leu Ser Ala Arg Ala Ser Val Leu Tyr Asn Pro Asn Trp Pro
245 250 255
Gly Tyr Ser Pro Val Phe Thr Gly Val Gly Glu Asn Tyr Ser Leu Asn
260 265 270
Val Glu Gly Ala Ser Ile Ser Ser Asn Thr Val Ser Leu Tyr Ser Tyr
275 280 285
Val Glu Phe Lys Glu Tyr Pro Leu Ser Thr Asp Thr Ala Asn Leu Val
290 295 300
Ala Val Pro Met Arg Glu Tyr Tyr Ser Thr Pro Ile Leu Arg Asp Gly
305 310 315 320
Thr Pro Ala Leu Asn Pro Ala Asp Ser Phe Thr Pro Tyr Ser Arg Phe
325 330 335
Ala Ser Val Asp His Asn Ala His Ser Met Ser Glu Glu Glu
340 345 350
<210> 37
<211> 404
<212> PRT
<213> 芽孢杆菌属的种
<400> 37
Val Lys Thr Lys Lys Trp Leu Arg Thr Leu Pro Ala Val Cys Leu Leu
1 5 10 15
Ser Ser Ala Met Leu Thr Val Pro Thr Ser Ser Phe Ala Ala Ile Asp
20 25 30
Met Ser Ser Cys Thr Lys Lys Asp Tyr Asn Gln Ser Cys Val Glu Gln
35 40 45
Thr Asp Pro Gly Val Asn Asn Arg Thr Tyr Ala Met Tyr Glu Thr Ile
50 55 60
Asn Arg Leu Val Glu Glu Asp Thr Leu Asn Glu Gln Thr Asn Trp Ser
65 70 75 80
Gly Lys Ser Trp Tyr Asn Phe Ile Arg Phe Asn Tyr Leu Ile Leu Pro
85 90 95
Ser Gln Glu Gln Glu Ile Leu His Gln Leu Asn Arg Ile Asp Pro Ser
100 105 110
Asn Val Glu Leu Ser Glu Val Leu Arg Ala Ile Asn Gly Tyr His Asn
115 120 125
Lys Thr Asn Leu Ile Gln Asp Tyr Ala Asn Thr Leu Lys Thr Thr Asp
130 135 140
Leu Asn Ala Thr Glu Tyr Val Glu Glu Pro Thr Val Asp Ser Thr Ser
145 150 155 160
Leu Gln Tyr Lys Glu Leu Asn Thr Val Leu Lys Asn Pro Ser Asn Glu
165 170 175
Ala Leu Thr Lys Thr Gly Gln Ile Ile Gly Gln Lys Ile Thr Ser Ser
180 185 190
Thr Gln Val Thr Asn Thr His Ser Ile Asp Val Gly Arg Lys Phe Ser
195 200 205
Met Lys Asn Ser Val Asn Ile Asp Val Ala Lys Phe Ile Lys Ala Gly
210 215 220
Val Glu Ile Thr Asp Glu Ile His Ala Asn Tyr Asn Tyr Thr Asn Gly
225 230 235 240
Thr Thr Thr Val Asn Thr Lys Glu Asp Ser Val Thr Thr Ser Pro Glu
245 250 255
Ser Val Phe Leu Lys Pro His Thr Gly Ala Val Ile Thr Ala Lys Leu
260 265 270
Ser Thr Pro Ala Tyr Ile Gly Gln Leu Lys Gly Glu Met Ala Leu Asp
275 280 285
Gly Tyr Tyr Thr Asp Gln Val Asn Phe Tyr Pro Thr Trp Val Gln Leu
290 295 300
Gly Ile Tyr Asn Lys Phe Lys Val Ile Ala Met His Asn Pro Asp Ile
305 310 315 320
Trp Glu Lys Leu Gln Glu Leu His Phe Ser Leu Asp Asp Thr Tyr Lys
325 330 335
Lys Val Asn Phe Glu Gly Lys Leu Thr Val Ser Glu Arg Lys Ala Pro
340 345 350
Gly Ser Ser Phe Glu Ser Ser Thr Gln Ile Tyr Glu Leu Asp Ala Lys
355 360 365
Gly Asp Ile Asn Tyr Asn Lys Pro Val Gly Val Pro Asp Thr Lys Thr
370 375 380
Gly Glu Val Asp Ile Gln Leu Asp Met Lys Lys Gln Lys Thr Leu Tyr
385 390 395 400
Leu Arg Thr Asn
<210> 38
<211> 1291
<212> PRT
<213> 芽孢杆菌属的种
<400> 38
Leu Thr Thr Leu Asn Glu Leu Tyr Pro Ser Tyr His Asn Val Leu Ala
1 5 10 15
Ser Pro Pro Val Ile Leu Ala Asp Asp Pro Asn Ser Ala Asn Gly Phe
20 25 30
Lys Asp Met Leu Lys Lys Leu Asp Asp Thr Trp Gly Glu Tyr Asp Lys
35 40 45
Asp Phe Leu Pro Lys Pro Val Thr Ala Ser Leu Ser Ser Leu Ile Asp
50 55 60
Leu Phe Asn Lys Lys Pro Gly Ser Ile Val Gln Phe Ile Gln Thr Gly
65 70 75 80
Ile Arg Leu Ala Phe Leu Ala Val Pro Gly Gly Gln Thr Tyr Ala Trp
85 90 95
Ala Leu Asn Lys Ile Ile Gly Leu Cys Phe Pro Thr Ser Gln Pro Asn
100 105 110
Leu Phe Glu Gln Ile Lys Glu Ala Val Gln Gln Leu Ile Asp Thr Ala
115 120 125
Leu Val Gln Thr Glu Ile Asn Lys Asn Val Asn Ala Leu Asn Gly Met
130 135 140
Thr Leu Pro Ile Arg Tyr Val Ser Asp Ala Met Gln Gln Ala Ile Gly
145 150 155 160
Lys Pro Thr Phe Ser Ser Ala Tyr Ala Gly Thr Asp Gly Cys Ser Thr
165 170 175
Asp Ala Ser Phe Ser Ser Thr Val Tyr Ala Ser Ser Cys Asp Ser Thr
180 185 190
Glu Cys Ser Cys Ser Asp Ala Pro Asp Arg Asn Phe Cys Thr Ala Asp
195 200 205
Pro Cys Pro Ser Pro Cys Lys Cys Arg Leu Asp Ala Val Tyr Asn Gln
210 215 220
Phe Asp Ala Ser Arg Lys Thr Val Glu Asn Ile Leu Thr Gly Met Arg
225 230 235 240
Thr Asn Leu Thr Thr Val Leu Lys Pro Glu Glu Met Thr Gln Tyr Met
245 250 255
Gln Ala Phe Leu Pro Leu Tyr Val Ala Gly Ala Thr Ile Met Leu Ser
260 265 270
Leu Tyr Arg Ser Tyr Ile Ala Phe Ala Gln Arg Tyr Glu Tyr Ala Leu
275 280 285
Gly Pro Asn Asp Thr Thr Lys Thr Thr Asn Asp Gln Leu Arg Val Lys
290 295 300
Ile Ser Glu Glu Ser Lys Tyr Val Tyr Glu Gln Phe Ser Lys Tyr Leu
305 310 315 320
Pro Ala Val Asp Ser Asn Thr Lys Gly Lys Leu Asn Ala Tyr Ile Gln
325 330 335
Tyr Thr Arg Asn Ile Thr Ile Asn Ala Leu Asp Gln Val Ser Thr Trp
340 345 350
Pro Phe Phe Asp Thr Trp Asp Tyr Pro Ser Ser Asp Lys Ala Gly Thr
355 360 365
Leu Ser Arg Pro Leu Ser Leu Ala Phe Asn Pro Thr Arg Ile Ile Phe
370 375 380
Asn Asp Ile Val Gly Pro Val Glu Cys Lys Thr Gly Ser Gln Asp Ser
385 390 395 400
Ser Leu Leu Asn Leu Asn Pro Leu Tyr Asp Ile Asp Gly Lys Ala Leu
405 410 415
Leu Ile Asn Thr Ile Thr Asn Tyr Phe Tyr Asp Asn Ile Gln Leu Lys
420 425 430
Ser Leu Asn Phe Gln Thr Tyr Asn His Asp Asp Asn Ile Tyr His Gln
435 440 445
His Phe Pro Leu Gly Phe Gln Ala Thr Tyr Ser Thr Arg Asp Asn Ser
450 455 460
Thr Leu Lys Val Asn His Thr Val Pro Asp Gly Ser Asn Ile Ser Leu
465 470 475 480
Gly Asn Asp Ile Phe Gly Ser Tyr Pro Ala Phe Asn Ile Ile Asn Met
485 490 495
Ser Ser Ser Gln Leu Asn Ala Gly Leu Ala Tyr Ser Asp Val Ser Asn
500 505 510
Ile Ser Phe Asp Thr Phe Ile Pro Ala Gly Cys Ser Ser Gln Asn Lys
515 520 525
Val Ser Tyr Pro Asp His Arg Ile Gln Ala Ile Tyr Pro Val Ile Pro
530 535 540
Asp Ser Ala Ser Arg Lys Lys Tyr Asp Ser Tyr Gly Asn Ser Asp Lys
545 550 555 560
Pro Gly Phe Ile Thr Thr Leu Ile Pro Asn Asp Thr Thr Thr Trp Ile
565 570 575
Thr Phe Thr Asn Leu Gly Ile Asn Thr Phe Ser Ala Glu Gln Thr Thr
580 585 590
Asn Thr Ser Gly Thr Lys Leu Val Glu Tyr Met Asn Gly Ala Ala Ala
595 600 605
Leu Lys Leu Gln Pro Gly Gln Phe Ala Gln Tyr Ser Ile Asn Thr Ser
610 615 620
Leu Phe Val Leu Pro Ser Asp Phe Lys Tyr Gln Val Arg Val Arg Ala
625 630 635 640
Ala Thr Thr Ala Thr Gly Ser Leu Ser Ile Gln Val Gly Glu Thr Pro
645 650 655
Ala Gln Thr Leu Gln Leu Pro Lys Thr Glu Asn Gln Pro Gly Gly Ile
660 665 670
Val Gly Lys Gln Gly Thr Tyr Met Pro Ile Pro Ala Ser His Ser Met
675 680 685
Asp Leu Thr Gln Arg Asp Thr Asn Leu Phe Thr Val Pro Ser Thr Ser
690 695 700
Ser Leu Ser Met Ser Ile Thr Asn Asn Ser Ala Thr Asp Ile Ile Leu
705 710 715 720
Asp Arg Ile Glu Phe Ile Pro Leu Gln Ala Ile Thr Pro Ser Asn Met
725 730 735
Ser Pro Arg Gln Phe Gly Pro Ala Ser Thr Glu Gln Thr Glu Thr Val
740 745 750
Ala Ile Arg Met Asp Val Tyr Tyr Gly Ile Lys Asp Ile Gln Ala Ile
755 760 765
Val Ser Ser Gly Gly Tyr Ala Lys Phe Arg Tyr Ser Arg His Ser Arg
770 775 780
Arg Gly Ser Phe Ile Val Tyr Glu Ser Thr Thr Asp Ser Asn Thr Trp
785 790 795 800
Asp Tyr Asp Gly Ser Phe Asp Thr Met Asp Ile Ile Tyr Leu Gly Thr
805 810 815
Val Asn Phe Leu Gly Gly Leu Ile Asp Tyr Tyr His Pro Ser Thr Ile
820 825 830
Glu Asp Leu Glu Asn Val Thr Glu Gln Val Tyr Ala Leu Phe Thr Ser
835 840 845
Ser Ser Ser Thr Glu Leu Ala Pro Thr Val Ser Ser Tyr Gln Ile Asn
850 855 860
Gln Val Ala Met Lys Val Arg Ala Leu Ser Asp Glu Val Phe Cys Lys
865 870 875 880
Glu Lys Ala Leu Leu Arg Lys Leu Val Asn Lys Ala Lys Gln Phe Leu
885 890 895
Lys Ala Arg Asn Leu Leu Val Gly Gly Asp Phe Glu Thr Leu Asn Lys
900 905 910
Trp Leu Leu Gly Thr Gln Ala Ile Ile Asn Asp Asp Ser Pro Leu Phe
915 920 925
Asn Gly Asp Tyr Leu Phe Leu Gln Pro Thr Asn Gly Ile Tyr Ser Ser
930 935 940
Tyr Ala Tyr Gln Lys Val Asp Glu Ser Lys Leu Lys Pro Tyr Thr Arg
945 950 955 960
Tyr Lys Val Ser Gly Phe Ile Gly Gln Ser Asn Arg Val Glu Leu Val
965 970 975
Val Ser Arg Tyr Gly Lys Glu Ile Asp Thr Ile Leu Asn Val Pro Tyr
980 985 990
Ala Ala Ala Leu Pro Ile Thr Ser Glu Pro Asn Pro Ser Cys Asp Asn
995 1000 1005
Ser Gln Pro Asp Ser His Phe Phe Ser Tyr Ser Ile Asp Val Gly
1010 1015 1020
Pro Leu His Pro Glu Leu Asn Pro Gly Ile Glu Phe Gly Leu Arg
1025 1030 1035
Ile Val Gln Pro Asn Gly Tyr Ala Thr Val Ser Asn Leu Glu Ile
1040 1045 1050
Met Glu Glu Arg Pro Leu Thr Asp Lys Glu Ile Gln Thr Ile Asn
1055 1060 1065
Arg Lys Asp Gln Lys Trp Glu Lys Ser Val Glu Gln Glu Arg Ala
1070 1075 1080
Lys Ile Ser Ala Leu Leu Gln Pro Thr Ile Asp Gln Ile Asn Ser
1085 1090 1095
Phe Phe Lys Asp Ala Asp Trp Asn Gly Thr Ile Leu Pro His Val
1100 1105 1110
Thr Tyr Gln Ser Leu Ala Ser Val Val Leu Pro Ala Leu Pro Lys
1115 1120 1125
Leu Arg His Trp Phe Met Asn Asp Arg Ala Gly Glu His Tyr His
1130 1135 1140
Ile Leu Gln Glu Leu Lys His Ala Leu Asp Tyr Val Phe Thr Gln
1145 1150 1155
Leu Glu Glu Gln Asn Leu Ile His Asn Gly Asn Phe Thr Asn Gly
1160 1165 1170
Leu Thr Asp Trp Leu Val Glu Gly Asp Ala Gln Ile Thr Leu Leu
1175 1180 1185
Ala Asn Asn Lys Pro Ala Leu Gln Leu Ser Asp Trp Asp Ala Ser
1190 1195 1200
Ala Ser Gln Ser Leu Asp Ile Ser Asp Phe Asp Glu Asn Lys Gln
1205 1210 1215
Tyr Lys Leu Arg Val Tyr Ala Lys Gly Thr Gly Thr Ile Thr Val
1220 1225 1230
Glu Asn Cys Glu Glu Ser Asn Pro Val Ser Phe Asp Thr Asp Thr
1235 1240 1245
Phe Thr Ser Gln Glu Gln Ile Phe Tyr Phe Asp Thr Pro Ser Phe
1250 1255 1260
Val Leu Arg Met Gln Ser Glu Gly Thr Glu Phe Val Val Gly Ser
1265 1270 1275
Val Glu Leu Ile Glu Leu Ser Asp Glu Asp Thr Asn Glu
1280 1285 1290
<210> 39
<211> 728
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 39
Leu Thr Thr Leu Asn Glu Leu Tyr Pro Ser Tyr His Asn Val Leu Ala
1 5 10 15
Ser Pro Pro Val Ile Leu Ala Asp Asp Pro Asn Ser Ala Asn Gly Phe
20 25 30
Lys Asp Met Leu Lys Lys Leu Asp Asp Thr Trp Gly Glu Tyr Asp Lys
35 40 45
Asp Phe Leu Pro Lys Pro Val Thr Ala Ser Leu Ser Ser Leu Ile Asp
50 55 60
Leu Phe Asn Lys Lys Pro Gly Ser Ile Val Gln Phe Ile Gln Thr Gly
65 70 75 80
Ile Arg Leu Ala Phe Leu Ala Val Pro Gly Gly Gln Thr Tyr Ala Trp
85 90 95
Ala Leu Asn Lys Ile Ile Gly Leu Cys Phe Pro Thr Ser Gln Pro Asn
100 105 110
Leu Phe Glu Gln Ile Lys Glu Ala Val Gln Gln Leu Ile Asp Thr Ala
115 120 125
Leu Val Gln Thr Glu Ile Asn Lys Asn Val Asn Ala Leu Asn Gly Met
130 135 140
Thr Leu Pro Ile Arg Tyr Val Ser Asp Ala Met Gln Gln Ala Ile Gly
145 150 155 160
Lys Pro Thr Phe Ser Ser Ala Tyr Ala Gly Thr Asp Gly Cys Ser Thr
165 170 175
Asp Ala Ser Phe Ser Ser Thr Val Tyr Ala Ser Ser Cys Asp Ser Thr
180 185 190
Glu Cys Ser Cys Ser Asp Ala Pro Asp Arg Asn Phe Cys Thr Ala Asp
195 200 205
Pro Cys Pro Ser Pro Cys Lys Cys Arg Leu Asp Ala Val Tyr Asn Gln
210 215 220
Phe Asp Ala Ser Arg Lys Thr Val Glu Asn Ile Leu Thr Gly Met Arg
225 230 235 240
Thr Asn Leu Thr Thr Val Leu Lys Pro Glu Glu Met Thr Gln Tyr Met
245 250 255
Gln Ala Phe Leu Pro Leu Tyr Val Ala Gly Ala Thr Ile Met Leu Ser
260 265 270
Leu Tyr Arg Ser Tyr Ile Ala Phe Ala Gln Arg Tyr Glu Tyr Ala Leu
275 280 285
Gly Pro Asn Asp Thr Thr Lys Thr Thr Asn Asp Gln Leu Arg Val Lys
290 295 300
Ile Ser Glu Glu Ser Lys Tyr Val Tyr Glu Gln Phe Ser Lys Tyr Leu
305 310 315 320
Pro Ala Val Asp Ser Asn Thr Lys Gly Lys Leu Asn Ala Tyr Ile Gln
325 330 335
Tyr Thr Arg Asn Ile Thr Ile Asn Ala Leu Asp Gln Val Ser Thr Trp
340 345 350
Pro Phe Phe Asp Thr Trp Asp Tyr Pro Ser Ser Asp Lys Ala Gly Thr
355 360 365
Leu Ser Arg Pro Leu Ser Leu Ala Phe Asn Pro Thr Arg Ile Ile Phe
370 375 380
Asn Asp Ile Val Gly Pro Val Glu Cys Lys Thr Gly Ser Gln Asp Ser
385 390 395 400
Ser Leu Leu Asn Leu Asn Pro Leu Tyr Asp Ile Asp Gly Lys Ala Leu
405 410 415
Leu Ile Asn Thr Ile Thr Asn Tyr Phe Tyr Asp Asn Ile Gln Leu Lys
420 425 430
Ser Leu Asn Phe Gln Thr Tyr Asn His Asp Asp Asn Ile Tyr His Gln
435 440 445
His Phe Pro Leu Gly Phe Gln Ala Thr Tyr Ser Thr Arg Asp Asn Ser
450 455 460
Thr Leu Lys Val Asn His Thr Val Pro Asp Gly Ser Asn Ile Ser Leu
465 470 475 480
Gly Asn Asp Ile Phe Gly Ser Tyr Pro Ala Phe Asn Ile Ile Asn Met
485 490 495
Ser Ser Ser Gln Leu Asn Ala Gly Leu Ala Tyr Ser Asp Val Ser Asn
500 505 510
Ile Ser Phe Asp Thr Phe Ile Pro Ala Gly Cys Ser Ser Gln Asn Lys
515 520 525
Val Ser Tyr Pro Asp His Arg Ile Gln Ala Ile Tyr Pro Val Ile Pro
530 535 540
Asp Ser Ala Ser Arg Lys Lys Tyr Asp Ser Tyr Gly Asn Ser Asp Lys
545 550 555 560
Pro Gly Phe Ile Thr Thr Leu Ile Pro Asn Asp Thr Thr Thr Trp Ile
565 570 575
Thr Phe Thr Asn Leu Gly Ile Asn Thr Phe Ser Ala Glu Gln Thr Thr
580 585 590
Asn Thr Ser Gly Thr Lys Leu Val Glu Tyr Met Asn Gly Ala Ala Ala
595 600 605
Leu Lys Leu Gln Pro Gly Gln Phe Ala Gln Tyr Ser Ile Asn Thr Ser
610 615 620
Leu Phe Val Leu Pro Ser Asp Phe Lys Tyr Gln Val Arg Val Arg Ala
625 630 635 640
Ala Thr Thr Ala Thr Gly Ser Leu Ser Ile Gln Val Gly Glu Thr Pro
645 650 655
Ala Gln Thr Leu Gln Leu Pro Lys Thr Glu Asn Gln Pro Gly Gly Ile
660 665 670
Val Gly Lys Gln Gly Thr Tyr Met Pro Ile Pro Ala Ser His Ser Met
675 680 685
Asp Leu Thr Gln Arg Asp Thr Asn Leu Phe Thr Val Pro Ser Thr Ser
690 695 700
Ser Leu Ser Met Ser Ile Thr Asn Asn Ser Ala Thr Asp Ile Ile Leu
705 710 715 720
Asp Arg Ile Glu Phe Ile Pro Leu
725
<210> 40
<211> 1176
<212> PRT
<213> 芽孢杆菌属的种
<400> 40
Leu Glu Phe Lys Phe Glu Trp Arg Tyr Phe Met Asn Ser Tyr Asn Lys
1 5 10 15
Asn Glu Tyr Glu Ile Val Asp Ser Pro Tyr Ser Ser Asn Asn Ser Asn
20 25 30
Leu Tyr Thr Asn Ser Arg Tyr Pro Tyr Val Asn Asn Pro Ser Gln Phe
35 40 45
Met Gln Asn Thr Thr Tyr Glu Asp Trp Leu Asn Leu Cys Lys Asp Asn
50 55 60
Ser Lys Tyr Asn Gln Asp Thr Asn Gly Phe Ile Asp Thr Pro Asp Thr
65 70 75 80
Lys Thr Ala Val Thr Ala Ala Ile Thr Val Val Gly Ala Ile Leu Thr
85 90 95
Tyr Ala Gly Val Pro Gly Ala Gly Ala Phe Thr Leu Leu Gly Lys Val
100 105 110
Val Pro Val Phe Trp Pro Ser Ala Ser Asp Lys Ser Gln Asn Ile Trp
115 120 125
Val Lys Phe Ile Glu Ser Ile Gln Asp Ile Leu Gly Lys Lys Leu Asp
130 135 140
Ile Asn Lys Arg Asn Lys Ala Ile Asp Lys Leu Glu Gly Leu Gln Ser
145 150 155 160
Val Ile Tyr Asn Tyr Asn Ala Ser Leu Lys Ser Trp Leu Glu Asn Lys
165 170 175
Asn Asn Ser Ser Phe Gln Thr Asp Val Val Lys Gly Asn Phe Ile Tyr
180 185 190
Ala Asn Asn Ala Phe Ile Glu Ser Met Asn Val Phe Thr Ser Ser Ser
195 200 205
Gly Asn Ser Lys Glu Lys Glu Glu Asn Val Leu Leu Leu Pro Ile Tyr
210 215 220
Thr His Ile Ala Asn Leu His Leu Leu Leu Leu Arg Asp Ala Ser Phe
225 230 235 240
Tyr Gly Lys Tyr Trp Gly Phe Ser Gly Ala Glu Ile Asn Asp Phe Tyr
245 250 255
Ser Lys Gln Gln Gln Asp Thr Ile Thr His Ile Ala His Cys Ile Lys
260 265 270
Tyr Tyr Asn Glu Gly Leu Thr Ile Ala Lys Glu Ala Phe Gln Leu Glu
275 280 285
Asp Lys Trp Asn Lys Phe Asn Ser Tyr Arg Arg Asn Met Thr Leu Lys
290 295 300
Val Leu Asp Val Ile Ser Leu Phe Ser Thr Tyr Asp Thr Lys Arg Tyr
305 310 315 320
Pro Ala Thr Ile Glu Lys Pro Asn Ile Asn Glu Leu Gly Lys Phe Glu
325 330 335
Leu Thr Arg Glu Val Tyr Thr Asp Ala Phe Ser Asp Pro Gly Ser Ser
340 345 350
Leu Asn Ser Ser Leu Thr Met Asp Glu Leu Asp Lys Lys Phe Thr Arg
355 360 365
Gly Pro His Leu Phe Asp Gln Leu Ile Lys Leu Phe Leu Tyr Thr Arg
370 375 380
Phe Ser His Asp Thr Phe Gly Phe Thr Gly Asn Lys Asn Cys Thr Ala
385 390 395 400
Leu Ile Asn Asn Asn Phe Cys Gln Glu Ala Gln Tyr Gly Asp Ile Gln
405 410 415
Lys Ala Asp Thr Glu Ser Thr Phe Ser Ile Asn Gln Leu Ile Asn Lys
420 425 430
Ile Asn Val Thr His Val Arg Asn Asn Pro Gly Ala Ile Ile Arg Met
435 440 445
Asp Phe Tyr Lys Glu Gln Asn Leu Leu Phe Gln Tyr Asn Ser Gly Ser
450 455 460
Lys Ala Thr Leu Val Asp Val Lys Thr Val Gln Ile Ser Asn Gln Ile
465 470 475 480
Asp Ser Ser Ile Pro Asn Ser Glu Thr Tyr Lys His Asn Phe Ser Tyr
485 490 495
Leu Lys Thr Gly Asn Asn Pro Thr Asn Pro Asn Leu Lys Ile Arg Gln
500 505 510
Val Ala Phe Ala Trp Thr His Trp Ser Val Asp Ser Asn Asn Thr Ile
515 520 525
Gln Glu Lys Val Ile Thr Ala Ile Pro Ile Ile Lys Ser Gln Ser Val
530 535 540
Asn Asp Thr Thr Ile Ile Pro Asn Pro Gly His Leu Gly Gly Asp Phe
545 550 555 560
Ile Gln Phe Asn Pro Gly Thr Ser Pro Arg Ile Glu Leu Gln Cys Arg
565 570 575
Ala Ser Asn Phe Pro Thr Thr Lys Asn Tyr Ala Val Arg Ile Arg Tyr
580 585 590
Val Ala Asn Gln Lys Phe Thr Val Ser Val Ser Ile Ala Gly Arg Ala
595 600 605
Thr Thr Phe Ile Thr Ala Asp Thr Met Ala Ala Ser Thr Gln Gly Lys
610 615 620
Tyr Leu Tyr Gln Gln Phe Ser Tyr Gly Asp Val Phe Thr Lys Ser Leu
625 630 635 640
Pro Val Pro Leu Ser Pro Arg Gln Leu Leu Thr Ile Ser Ile Thr Thr
645 650 655
Leu Ser Pro Ile Asn Gln Asn Thr Ile Phe Ala Ile Asp Arg Ile Glu
660 665 670
Phe Ile Pro Val Asp Glu Phe Pro Thr Leu Thr Thr Glu Gln Gln Ser
675 680 685
Ile Glu Thr Leu Gln Asn Gln Ile Asn Asn Leu Phe Thr Asp Tyr Thr
690 695 700
Gln Asn Thr Leu Lys Ser Glu Val Thr Asp Tyr Gln Ile Asp Gln Ile
705 710 715 720
Ala Thr Thr Val Asp Thr Val Ser Glu Glu Gln Tyr Ser Gln Glu Lys
725 730 735
Met Ile Leu Leu Asp Glu Ile Lys His Ala Lys Gln Leu Ser Tyr Ala
740 745 750
Arg Asn Leu Leu Gln Asn Gly Asp Phe Gln Asp Phe Leu Gly Trp Thr
755 760 765
Thr Asn Asn Glu Val Thr Ile Gln Thr Lys Asn Pro Val Phe Lys Glu
770 775 780
Tyr Ala Leu Tyr Met Pro Gly Ala Arg Val Ile Asn Thr Ser Val Phe
785 790 795 800
Pro Thr Tyr Leu Tyr Gln Lys Ile Asp Glu Ser Lys Leu Lys Pro Tyr
805 810 815
Thr Arg Tyr Leu Val Arg Gly Phe Ile Glu Ser Ser Lys Gly Leu Glu
820 825 830
Val Ser Ile Thr Arg Tyr Asn Lys Asp Ile Asn Thr Ile Met Asn Val
835 840 845
Pro Asn Asp Val Ile Thr Ile Gln Ser Tyr Glu Gln Thr Glu Asn Thr
850 855 860
Thr Thr Gln Val Leu Pro Asn Ser Ile Arg Ser Gln Asn Ser Cys Thr
865 870 875 880
Cys Glu Ile Thr Thr Leu Pro Ala Ile Cys Gln Asp Pro His Ala Phe
885 890 895
Ser Phe Phe Ile Asp Thr Gly Asp Leu His Tyr Asn Glu Asn Leu Gly
900 905 910
Ile Ser Ile Gly Phe Lys Ile Ser Asn Pro Asp Gly Tyr Ala Thr Leu
915 920 925
Gly Asn Val Glu Ile Ile Glu Gly Gln Pro Leu Val Asp Gln Glu Ile
930 935 940
Lys Tyr Val Thr Glu Lys Glu Asn Lys Trp Lys Gln Ile Asn Lys Gln
945 950 955 960
Gln Gln Ile Glu Thr Glu Gln Thr Tyr Ser Glu Ala Gln Gln Ala Val
965 970 975
Asn Asn Leu Phe Leu Asn Gly Pro Ser Ser Met Leu Gln Phe Gln Thr
980 985 990
Thr Gln Leu Asp Ile Ile Lys Ala Asp Asn Leu Ile Asn Gln Ile Pro
995 1000 1005
Tyr Val Tyr Asn Ala Trp Leu Pro Asn Glu Pro Gly Val Asn Tyr
1010 1015 1020
Asn Leu Phe Met Asn Leu Lys Asn Gln Ile Ser Gln Ala His Ala
1025 1030 1035
Leu Tyr Thr Asn Arg Asn Ile Ile Gln Asn Gly Asn Phe Met Asn
1040 1045 1050
Asp Thr Thr Asn Trp Phe Thr Ser Pro Asp Val Asn Ile Gln Gln
1055 1060 1065
Thr Asp Asp Gly Ser Val Leu Val Leu Thr Asn Trp Ser Ala Gln
1070 1075 1080
Ala Ser Gln Thr Ile Gln Leu Gln Ser Asn His Arg Tyr Val Leu
1085 1090 1095
Arg Val Ile Ala Lys Lys Glu Gly Gly Gly Thr Gly Tyr Val Lys
1100 1105 1110
Ile Ser Asp Cys Thr Asn Gln Ile Gln Lys Leu Glu Phe His Thr
1115 1120 1125
Tyr Ser Asn Cys Asp Ala Cys Thr Asp Phe Leu Asp Tyr Val Thr
1130 1135 1140
Lys Asp Ile Ile Ile Thr Pro Asn Thr Asn Gln Ile Arg Leu Asp
1145 1150 1155
Ile Gly Glu Thr Glu Gly Val Phe Lys Ile Arg Ser Ile Glu Leu
1160 1165 1170
Ile Cys Ser
1175
<210> 41
<211> 676
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 41
Leu Glu Phe Lys Phe Glu Trp Arg Tyr Phe Met Asn Ser Tyr Asn Lys
1 5 10 15
Asn Glu Tyr Glu Ile Val Asp Ser Pro Tyr Ser Ser Asn Asn Ser Asn
20 25 30
Leu Tyr Thr Asn Ser Arg Tyr Pro Tyr Val Asn Asn Pro Ser Gln Phe
35 40 45
Met Gln Asn Thr Thr Tyr Glu Asp Trp Leu Asn Leu Cys Lys Asp Asn
50 55 60
Ser Lys Tyr Asn Gln Asp Thr Asn Gly Phe Ile Asp Thr Pro Asp Thr
65 70 75 80
Lys Thr Ala Val Thr Ala Ala Ile Thr Val Val Gly Ala Ile Leu Thr
85 90 95
Tyr Ala Gly Val Pro Gly Ala Gly Ala Phe Thr Leu Leu Gly Lys Val
100 105 110
Val Pro Val Phe Trp Pro Ser Ala Ser Asp Lys Ser Gln Asn Ile Trp
115 120 125
Val Lys Phe Ile Glu Ser Ile Gln Asp Ile Leu Gly Lys Lys Leu Asp
130 135 140
Ile Asn Lys Arg Asn Lys Ala Ile Asp Lys Leu Glu Gly Leu Gln Ser
145 150 155 160
Val Ile Tyr Asn Tyr Asn Ala Ser Leu Lys Ser Trp Leu Glu Asn Lys
165 170 175
Asn Asn Ser Ser Phe Gln Thr Asp Val Val Lys Gly Asn Phe Ile Tyr
180 185 190
Ala Asn Asn Ala Phe Ile Glu Ser Met Asn Val Phe Thr Ser Ser Ser
195 200 205
Gly Asn Ser Lys Glu Lys Glu Glu Asn Val Leu Leu Leu Pro Ile Tyr
210 215 220
Thr His Ile Ala Asn Leu His Leu Leu Leu Leu Arg Asp Ala Ser Phe
225 230 235 240
Tyr Gly Lys Tyr Trp Gly Phe Ser Gly Ala Glu Ile Asn Asp Phe Tyr
245 250 255
Ser Lys Gln Gln Gln Asp Thr Ile Thr His Ile Ala His Cys Ile Lys
260 265 270
Tyr Tyr Asn Glu Gly Leu Thr Ile Ala Lys Glu Ala Phe Gln Leu Glu
275 280 285
Asp Lys Trp Asn Lys Phe Asn Ser Tyr Arg Arg Asn Met Thr Leu Lys
290 295 300
Val Leu Asp Val Ile Ser Leu Phe Ser Thr Tyr Asp Thr Lys Arg Tyr
305 310 315 320
Pro Ala Thr Ile Glu Lys Pro Asn Ile Asn Glu Leu Gly Lys Phe Glu
325 330 335
Leu Thr Arg Glu Val Tyr Thr Asp Ala Phe Ser Asp Pro Gly Ser Ser
340 345 350
Leu Asn Ser Ser Leu Thr Met Asp Glu Leu Asp Lys Lys Phe Thr Arg
355 360 365
Gly Pro His Leu Phe Asp Gln Leu Ile Lys Leu Phe Leu Tyr Thr Arg
370 375 380
Phe Ser His Asp Thr Phe Gly Phe Thr Gly Asn Lys Asn Cys Thr Ala
385 390 395 400
Leu Ile Asn Asn Asn Phe Cys Gln Glu Ala Gln Tyr Gly Asp Ile Gln
405 410 415
Lys Ala Asp Thr Glu Ser Thr Phe Ser Ile Asn Gln Leu Ile Asn Lys
420 425 430
Ile Asn Val Thr His Val Arg Asn Asn Pro Gly Ala Ile Ile Arg Met
435 440 445
Asp Phe Tyr Lys Glu Gln Asn Leu Leu Phe Gln Tyr Asn Ser Gly Ser
450 455 460
Lys Ala Thr Leu Val Asp Val Lys Thr Val Gln Ile Ser Asn Gln Ile
465 470 475 480
Asp Ser Ser Ile Pro Asn Ser Glu Thr Tyr Lys His Asn Phe Ser Tyr
485 490 495
Leu Lys Thr Gly Asn Asn Pro Thr Asn Pro Asn Leu Lys Ile Arg Gln
500 505 510
Val Ala Phe Ala Trp Thr His Trp Ser Val Asp Ser Asn Asn Thr Ile
515 520 525
Gln Glu Lys Val Ile Thr Ala Ile Pro Ile Ile Lys Ser Gln Ser Val
530 535 540
Asn Asp Thr Thr Ile Ile Pro Asn Pro Gly His Leu Gly Gly Asp Phe
545 550 555 560
Ile Gln Phe Asn Pro Gly Thr Ser Pro Arg Ile Glu Leu Gln Cys Arg
565 570 575
Ala Ser Asn Phe Pro Thr Thr Lys Asn Tyr Ala Val Arg Ile Arg Tyr
580 585 590
Val Ala Asn Gln Lys Phe Thr Val Ser Val Ser Ile Ala Gly Arg Ala
595 600 605
Thr Thr Phe Ile Thr Ala Asp Thr Met Ala Ala Ser Thr Gln Gly Lys
610 615 620
Tyr Leu Tyr Gln Gln Phe Ser Tyr Gly Asp Val Phe Thr Lys Ser Leu
625 630 635 640
Pro Val Pro Leu Ser Pro Arg Gln Leu Leu Thr Ile Ser Ile Thr Thr
645 650 655
Leu Ser Pro Ile Asn Gln Asn Thr Ile Phe Ala Ile Asp Arg Ile Glu
660 665 670
Phe Ile Pro Val
675
<210> 42
<211> 405
<212> PRT
<213> 芽孢杆菌属的种
<400> 42
Met Lys Lys Thr Pro Tyr Lys Val Met Ala Leu Ser Ala Leu Leu Ala
1 5 10 15
Val Val Thr Ala Ser Asn Ile Val Pro Thr His Thr Val Ala Ala Glu
20 25 30
Asn Thr Val Gln Thr Ile Gln Val Ser Asn Met Lys Lys Asn Met Thr
35 40 45
Ser Tyr Lys Asp Phe Glu Tyr Gln Leu Gly Pro Glu Gly Leu Arg Glu
50 55 60
Ala Met Ala Thr Thr Gly Ser Asn Ala Leu Val Met Asp Leu Tyr Ala
65 70 75 80
Leu Thr Leu Ile Lys Gln Gln Asn Ala Asp Phe Thr Asn Val Asp Ile
85 90 95
Ile Asp Glu Lys Leu Arg Ala Ala Ile Thr Thr His Gln Asn Val Gly
100 105 110
Arg Ser Asn Ala Thr Lys Trp Leu Asp Met Leu Lys Pro Lys Leu Ile
115 120 125
Thr Thr Asn Gln Asn Ile Ile Asn Tyr Asn Thr Lys Phe Gln Asn Tyr
130 135 140
Tyr Asp Thr Leu Leu Asp Ala Val Asp Ala Thr Asp Lys Ala Thr Leu
145 150 155 160
Lys Lys Gly Leu Ser Asn Leu Asn Thr Ser Ile Ser Ile Tyr Lys Lys
165 170 175
Asp Val Asp Asn Leu Ile Ala Glu Leu Ile Ser Phe Arg Asp Arg Met
180 185 190
Ala Thr Asp Thr Gln Asn Phe Lys Ala Asp Thr Ser Ser Leu Val Ser
195 200 205
Ile Leu Thr Ser Glu Asp Ser Gly Leu Pro Ile Leu Gln Gln Gln Ile
210 215 220
Asp Ala Tyr Asn Ala Ala Ile Ala Lys Asp Asn Ala Ile Ile Ile Gly
225 230 235 240
Gly Ser Val Ala Leu Gly Leu Gly Ala Val Gly Ile Ile Gly Gly Gly
245 250 255
Ile Leu Ile Gly Thr Gly Ala Gly Ser Gly Leu Gly Val Thr Leu Val
260 265 270
Val Gly Gly Val Ser Ala Ile Ala Gly Gly Thr Thr Ala Ile Val Leu
275 280 285
Ala Lys Gln Asn Ile Asp Ser Ser Arg Glu Gly Ile Arg Asp Leu Thr
290 295 300
Leu Asn Leu Asn Asp Thr Lys Ala Gln Met Val Val Val Asn Asn Ile
305 310 315 320
Lys Asn Gln Cys Ile Tyr Leu Ser Asp Thr Ile Asp Leu Ala Ile Val
325 330 335
Ala Leu Gln Asn Ile Ser Asn Gln Trp His Ser Met Ser Ala Arg Tyr
340 345 350
Thr Thr Leu Ile Glu Asn Val Asp Ser Ile Asn Pro Asn Glu Phe Ala
355 360 365
Phe Ile Lys Glu Asp Leu His Val Ala Ser Asp Ser Trp Lys Asp Ile
370 375 380
Lys Glu Tyr Ala Asp Lys Leu Tyr Thr Asp Ile Lys Val Val Glu Thr
385 390 395 400
Asp Gly Pro Glu Leu
405
<210> 43
<211> 370
<212> PRT
<213> 芽孢杆菌属的种
<400> 43
Met Thr Asn Gly Asn Lys Asn Asn Glu Asp Ser Val Gln Asn Glu Ile
1 5 10 15
Lys Ser Ile Val Asp Phe Pro Asp Phe Asn Ile Tyr Lys Ile Arg Thr
20 25 30
Asn Tyr Asn Lys Tyr Val Asp Val Ser Gly Asp Pro Asn Asp Asn Phe
35 40 45
Ala Pro Ile Val Gln His Asp Gly His Asn Gly Asn Asn Gln Lys Phe
50 55 60
Leu Ile Tyr Thr Leu Asp Asn Gly Tyr Ser Val Ile Ala Ala Arg His
65 70 75 80
Ser Gly Lys Val Val Asp Val Arg Tyr Ile Asn Phe Pro Ser Pro Phe
85 90 95
Pro Pro Pro Ile Lys Asp His Leu Ile Gln His Asp Phe Lys Asn Gly
100 105 110
Asp Asn Gln Lys Phe Arg Leu Ala Thr Glu Gly Thr Ile Glu Ile Gln
115 120 125
His Asp Gly Gln Val Trp Asp Val Glu Gly Ser Gly Asn Lys Ala Gln
130 135 140
Ile Ile Pro Tyr His Tyr His Gly Gly Ser Asn Gln Lys Phe Arg Leu
145 150 155 160
Glu Lys Ser Gly Ser Val Ser Ile Lys Thr Pro Asn Ile Glu Ile Leu
165 170 175
Pro Ser Ala Pro Asp Phe Lys Thr Asp Asp Leu Asn Glu Glu Leu Pro
180 185 190
Asp Glu Thr Thr Pro Val Val Thr His Val Thr Tyr Leu Pro Tyr Phe
195 200 205
Met Val Lys Asp Ser Tyr Tyr Asn Pro Leu Gln Lys Ile Gln Asp Ser
210 215 220
Pro Tyr Tyr Val Leu Val Arg Arg Gln Phe Trp Lys Lys Ile Thr His
225 230 235 240
Arg Lys Leu Ala Pro Ser Glu Ala Tyr Asp Tyr Glu Gln Ile Thr Gly
245 250 255
Val Ser Arg Thr Asp Gln Thr Ser Met Thr Glu Thr Thr Glu Ile Ser
260 265 270
Ile Gly Ala Asp Leu Gly Phe Met Phe Lys Gly Phe Ser Leu Gly Leu
275 280 285
Ser Thr Ser Ile Thr Lys Ala Leu Ser Val Thr Lys Ser Thr Ser Asn
290 295 300
Thr Glu Ser Thr Glu Lys Lys Glu Gly Val Phe Tyr Lys Asn Pro Phe
305 310 315 320
Asn Tyr Gln Ile Ala Tyr Ala Arg Tyr Met Leu Ile Asn Glu Tyr Tyr
325 330 335
Val Thr Arg Ala Asn Gly Glu Met Ile Thr Thr Gly Asp Ala Ala Tyr
340 345 350
Trp Arg Val Pro Asn Pro Glu Arg Thr Val Ser Gln Ile Ile Pro Arg
355 360 365
Lys Ser
370
<210> 44
<211> 342
<212> PRT
<213> 芽孢杆菌属的种
<400> 44
Met Lys Asn Ile Lys Asn Met Lys Arg Lys Ile Ala Ala Thr Ser Ser
1 5 10 15
Ile Ile Thr Leu Ala Thr Ser Phe Ala Phe Thr Ser Pro Glu Leu Ala
20 25 30
Lys Ala Asp Ser Asn Asn Ile Val Asn Lys Val Pro Glu Gln Gln Gln
35 40 45
Pro Leu Lys Ser Gln Asp Pro Val Ser Val Pro Ser Glu Asp Val Lys
50 55 60
Gly Leu Gln Asp Leu Trp Gln Arg Lys Asp Ile Lys Thr Ala Leu Ala
65 70 75 80
Met Val Ala Ser Val Ser Phe Asp Asp Leu Arg Ser Ile Gly Gly Phe
85 90 95
Asn Phe Leu Tyr Tyr Gln Lys Pro Ser Tyr Ser Val Glu Leu Ile Gly
100 105 110
Asn Pro Lys Asn Glu Pro Ser Ser Pro Val Tyr Leu Gly Thr Ser Asn
115 120 125
Leu Ser His Ser Gly Gly Thr Ser Glu Gln Thr Leu Ser Ser Ala Ala
130 135 140
Phe Ser Lys Ala Val Thr Ser Thr Val Ser Ala Thr Thr Thr His Gly
145 150 155 160
Ala Lys Val Gly Ala Lys Ala Gly Ala Lys Leu Gln Val Pro Leu Val
165 170 175
Ala Glu Ala Ser Val Glu Leu Asn Ala Glu Tyr Asn Phe Ala Asn Ala
180 185 190
Gly Thr Glu Thr Asn Thr Glu Thr Val Thr Tyr Thr Val Pro Ser Gln
195 200 205
Asn Val Lys Leu Lys Pro Gly Glu Thr Ala Lys Val Thr Ala Ser Leu
210 215 220
Arg Met Ile Glu Ala Ser Gly Asp Val Asn Leu Arg Thr Thr Tyr Thr
225 230 235 240
Gly Lys Val Glu Ser Asn Tyr Glu Arg Tyr Ile Gly His Gly Met Gly
245 250 255
Glu Ile Ser Asp Thr Asn Lys Ser Gly Leu Gly Ser Trp Ile Gln Ala
260 265 270
Leu Ala Phe Leu Asp Ser Asp Leu Asn Asn Tyr Trp Ser Tyr Ser Asn
275 280 285
Ser Asp His Asp Val Val Tyr Gln Asp Gly Lys Gly Lys Phe Lys Ala
290 295 300
Thr Tyr Gly Thr Leu Val Asp Ile Lys Val Glu Ile Ser Lys Glu Thr
305 310 315 320
Asp Ser Lys Asp Asn Thr Pro Ile Arg Ser Tyr Ser Tyr Thr Val Thr
325 330 335
Pro Glu Ile Thr Lys Lys
340
<210> 45
<211> 326
<212> PRT
<213> 芽孢杆菌属的种
<400> 45
Met Lys Lys Lys Asn Asn Lys Lys Thr Lys Gln Ile Leu Ser Leu Ala
1 5 10 15
Met Leu Gly Ala Ile Gly Thr Ser Leu Ala Phe Thr Ser Pro Gly Ser
20 25 30
Val Ser Ala Ala Glu Ile Asn Ser Ile Gln Ser Ala Ala Gln Glu Gln
35 40 45
Gly Ser Val Ser Ile Ser Asp Trp Arg Glu Pro Phe Arg Asn Ala Tyr
50 55 60
Lys Ile Ala Gln Lys His Asp Pro Tyr Thr Tyr Phe Gly Glu Asp Ala
65 70 75 80
Thr Glu Tyr Thr Leu Gly Asn Val Tyr Gln Ser Ser Val Glu Ala Asp
85 90 95
Gly Ser Pro Thr Ile Thr Gly Ser Lys Ser Leu Phe Ala Gly Arg Thr
100 105 110
Thr Leu Thr Asn Asp Ser Asp Gln Glu Gln Thr Leu Thr Thr Asp Gln
115 120 125
Phe Ser Lys Thr Phe Glu Asn Ser Val Thr Asn Ser Thr Thr Asn Gly
130 135 140
Phe Asn Leu Gly Val Ser Ala Ser Ser Ser Phe Gly Ile Pro Leu Ile
145 150 155 160
Gly Glu Thr Ser Val Glu Ile Ser Thr Glu Tyr Asn Phe Ser Ser Thr
165 170 175
Glu Glu Lys Thr Lys Ser Glu Ser Tyr Thr Tyr Thr Ala Ser Pro Gln
180 185 190
Asn Ile Lys Val Pro Ala His Ser Ser Val Glu Val Ile Val Asn Leu
195 200 205
Asn Thr Ala Lys Ile Ser Gly Asn Val Lys Leu Leu Ser Lys Ile Asp
210 215 220
Gly Thr Phe Thr His Ala Ser Gly Gly Asp Gly Ser Asn Thr Ala Ser
225 230 235 240
Arg Gly Leu Trp Val Leu Glu Tyr Asp Ala Ser Pro Tyr Glu Lys Leu
245 250 255
Ser Asp Leu Lys Ser Ala Arg Val Gly Ala Glu Ala Tyr Gly Pro Val
260 265 270
Tyr Leu Val Gly Ser Gly Lys Tyr Ser Ala Glu Tyr Gly Thr Glu Phe
275 280 285
Ala Val Thr Val Arg Pro Val Glu Lys Pro Asn Gly Phe Lys Ser Met
290 295 300
Ile Ala Glu Asn Ser Lys Ser Thr Asn Glu Gly Tyr Thr Tyr Lys Val
305 310 315 320
Lys Pro Glu Ile Lys Lys
325
<210> 46
<211> 632
<212> PRT
<213> 芽孢杆菌属的种
<400> 46
Met Asn Arg Asn Lys Asp Cys Asn Cys Asn Asp Gly Phe Gln Lys Ser
1 5 10 15
Asp Met Ser Asp Arg Tyr Pro Tyr Ala Asn Asp Thr Lys Ala Ala Leu
20 25 30
Gln Asn Met Asp Tyr Lys Asp Trp Leu Val Met Asn Gln Gly Glu Glu
35 40 45
Leu Asn Gly Leu Asp Pro Lys Asp Gly Val Asn Ala Ala Val Ala Met
50 55 60
Val Gly Thr Leu Met Trp Thr Phe Gly Asn Pro Ala Phe Thr Gly Pro
65 70 75 80
Leu Gly Gly Val Ile Cys Ala Phe Gly Leu Leu Ile Pro Ile Leu Trp
85 90 95
Pro Asp Asp Glu Glu Pro Gln Lys Val Trp Glu Asp Leu Ile Asn His
100 105 110
Gly Asp Lys Phe Phe Asp Glu Lys Ile Gly Lys Ile Glu Lys Ala Arg
115 120 125
Ala Lys Thr Phe Ile Ala Gly Met Lys Asn Glu Leu Gly Lys Tyr Glu
130 135 140
Lys Ala Leu Glu Asp Trp Lys Ala Asn Lys Asn Gln Asp Thr Ala Ala
145 150 155 160
Thr Val Gln Glu Glu Phe Asp Asp Ala Gly Asp Tyr Leu Asp Thr Lys
165 170 175
Met Glu Glu Leu Lys Ile Glu Gly Tyr Glu Gln Thr Leu Leu Ser Cys
180 185 190
Tyr Ala Gln Ala Ala Leu Met His Leu Asn Leu Leu Ser Gln Gly Val
195 200 205
Gln Tyr Ala Asp Glu Trp Arg Lys Asn Asn Pro Gly Gln Thr Ser Lys
210 215 220
Tyr Trp Arg Glu Met Leu Thr Lys Gly Asp Lys Asn Arg Arg Gly Arg
225 230 235 240
Ile Glu Ala Tyr Met Asp Tyr Cys Thr Lys Thr Tyr Arg Glu Gly Leu
245 250 255
Asn Lys Val Lys Asn Val Pro Asn Ile Thr Trp Tyr Thr Tyr Asn Ser
260 265 270
Tyr Arg Arg Glu Met Thr Leu Ala Val Leu Asp Leu Ile Ala Leu Phe
275 280 285
Pro Asn Tyr Asp Val Asp Arg Tyr Pro Lys Gly Thr Lys Ser Glu Leu
290 295 300
Thr Arg Glu Ile Tyr Met Asp Asn Gly Tyr Thr Lys Asp Val Asp Val
305 310 315 320
Gly Asp Leu Pro Glu Leu Asp Gln Ala Leu Thr Arg Arg Pro His Leu
325 330 335
Phe Asp Ser Leu Ala Glu Leu Lys Leu Ile Pro Ser Asn Val Gly Leu
340 345 350
Ala His Cys Leu Ile Lys Ser Arg Arg Ile Asn Asn Asp Val Leu Val
355 360 365
Gly Tyr Asp Leu Pro Gly Ser Arg His Leu Ser Ser Leu Pro Ile Ile
370 375 380
Gly Val Asn Val Glu Trp Arg Glu Lys His Phe His Gly Gly Lys Leu
385 390 395 400
Cys Asp Arg Ala Asn Thr Gly Ile Glu Ile Leu Thr Trp Ile Pro Asp
405 410 415
Phe Gly Val Thr Gln Ser Ser Ile Phe Lys Met Glu Asp Gly Gly Gly
420 425 430
Cys Ile Glu His Gly Asn Gly Ile Gln Pro Pro Arg Lys Asn Ala Lys
435 440 445
Arg Asp Pro Leu Gly Gly Tyr Ser His Arg Leu Ser Tyr Met Ser Val
450 455 460
Arg Glu Ala Gln Asn Pro Ser Phe Tyr Met Tyr Ser Tyr Ala Trp Thr
465 470 475 480
His Leu Ser Val Asp Glu Lys Asn Thr Ile Ala Lys Asp Thr Ile Thr
485 490 495
Gln Ile Pro Ala Val Lys Ala Val Glu Gln Asn Asn Asp Phe Val Val
500 505 510
Ile Ala Gly Pro Gly His Thr Gly Gly Asp Leu Val Lys Leu Asn Gly
515 520 525
Ile Thr Lys Gln Gly Leu Lys Leu Lys Cys Val Phe Pro Gln Asn Lys
530 535 540
Lys Tyr Lys Ile Arg Leu Arg Tyr Ala Ala Asp Asn Asp Thr Arg Val
545 550 555 560
Asn Ile Ala Val Pro Asp Ser Asp Gly Thr Thr Ala Tyr Trp Ile Asp
565 570 575
Ala Lys Lys Thr Tyr Pro Gly Val Glu Leu Lys Tyr Asn Asn Phe Lys
580 585 590
Tyr Leu Asp Thr Asp Ala Ile Val Asn Thr Pro Asn Lys Ala Val Thr
595 600 605
Ile Ala Ile Lys Asn Ala Leu Lys Asp Thr Ser Ile Ile Leu Asp Arg
610 615 620
Ile Glu Phe Ile Pro Gln Glu Gly
625 630
<210> 47
<211> 630
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 47
Met Asn Arg Asn Lys Asp Cys Asn Cys Asn Asp Gly Phe Gln Lys Ser
1 5 10 15
Asp Met Ser Asp Arg Tyr Pro Tyr Ala Asn Asp Thr Lys Ala Ala Leu
20 25 30
Gln Asn Met Asp Tyr Lys Asp Trp Leu Val Met Asn Gln Gly Glu Glu
35 40 45
Leu Asn Gly Leu Asp Pro Lys Asp Gly Val Asn Ala Ala Val Ala Met
50 55 60
Val Gly Thr Leu Met Trp Thr Phe Gly Asn Pro Ala Phe Thr Gly Pro
65 70 75 80
Leu Gly Gly Val Ile Cys Ala Phe Gly Leu Leu Ile Pro Ile Leu Trp
85 90 95
Pro Asp Asp Glu Glu Pro Gln Lys Val Trp Glu Asp Leu Ile Asn His
100 105 110
Gly Asp Lys Phe Phe Asp Glu Lys Ile Gly Lys Ile Glu Lys Ala Arg
115 120 125
Ala Lys Thr Phe Ile Ala Gly Met Lys Asn Glu Leu Gly Lys Tyr Glu
130 135 140
Lys Ala Leu Glu Asp Trp Lys Ala Asn Lys Asn Gln Asp Thr Ala Ala
145 150 155 160
Thr Val Gln Glu Glu Phe Asp Asp Ala Gly Asp Tyr Leu Asp Thr Lys
165 170 175
Met Glu Glu Leu Lys Ile Glu Gly Tyr Glu Gln Thr Leu Leu Ser Cys
180 185 190
Tyr Ala Gln Ala Ala Leu Met His Leu Asn Leu Leu Ser Gln Gly Val
195 200 205
Gln Tyr Ala Asp Glu Trp Arg Lys Asn Asn Pro Gly Gln Thr Ser Lys
210 215 220
Tyr Trp Arg Glu Met Leu Thr Lys Gly Asp Lys Asn Arg Arg Gly Arg
225 230 235 240
Ile Glu Ala Tyr Met Asp Tyr Cys Thr Lys Thr Tyr Arg Glu Gly Leu
245 250 255
Asn Lys Val Lys Asn Val Pro Asn Ile Thr Trp Tyr Thr Tyr Asn Ser
260 265 270
Tyr Arg Arg Glu Met Thr Leu Ala Val Leu Asp Leu Ile Ala Leu Phe
275 280 285
Pro Asn Tyr Asp Val Asp Arg Tyr Pro Lys Gly Thr Lys Ser Glu Leu
290 295 300
Thr Arg Glu Ile Tyr Met Asp Asn Gly Tyr Thr Lys Asp Val Asp Val
305 310 315 320
Gly Asp Leu Pro Glu Leu Asp Gln Ala Leu Thr Arg Arg Pro His Leu
325 330 335
Phe Asp Ser Leu Ala Glu Leu Lys Leu Ile Pro Ser Asn Val Gly Leu
340 345 350
Ala His Cys Leu Ile Lys Ser Arg Arg Ile Asn Asn Asp Val Leu Val
355 360 365
Gly Tyr Asp Leu Pro Gly Ser Arg His Leu Ser Ser Leu Pro Ile Ile
370 375 380
Gly Val Asn Val Glu Trp Arg Glu Lys His Phe His Gly Gly Lys Leu
385 390 395 400
Cys Asp Arg Ala Asn Thr Gly Ile Glu Ile Leu Thr Trp Ile Pro Asp
405 410 415
Phe Gly Val Thr Gln Ser Ser Ile Phe Lys Met Glu Asp Gly Gly Gly
420 425 430
Cys Ile Glu His Gly Asn Gly Ile Gln Pro Pro Arg Lys Asn Ala Lys
435 440 445
Arg Asp Pro Leu Gly Gly Tyr Ser His Arg Leu Ser Tyr Met Ser Val
450 455 460
Arg Glu Ala Gln Asn Pro Ser Phe Tyr Met Tyr Ser Tyr Ala Trp Thr
465 470 475 480
His Leu Ser Val Asp Glu Lys Asn Thr Ile Ala Lys Asp Thr Ile Thr
485 490 495
Gln Ile Pro Ala Val Lys Ala Val Glu Gln Asn Asn Asp Phe Val Val
500 505 510
Ile Ala Gly Pro Gly His Thr Gly Gly Asp Leu Val Lys Leu Asn Gly
515 520 525
Ile Thr Lys Gln Gly Leu Lys Leu Lys Cys Val Phe Pro Gln Asn Lys
530 535 540
Lys Tyr Lys Ile Arg Leu Arg Tyr Ala Ala Asp Asn Asp Thr Arg Val
545 550 555 560
Asn Ile Ala Val Pro Asp Ser Asp Gly Thr Thr Ala Tyr Trp Ile Asp
565 570 575
Ala Lys Lys Thr Tyr Pro Gly Val Glu Leu Lys Tyr Asn Asn Phe Lys
580 585 590
Tyr Leu Asp Thr Asp Ala Ile Val Asn Thr Pro Asn Lys Ala Val Thr
595 600 605
Ile Ala Ile Lys Asn Ala Leu Lys Asp Thr Ser Ile Ile Leu Asp Arg
610 615 620
Ile Glu Phe Ile Pro Gln
625 630
<210> 48
<211> 218
<212> PRT
<213> 芽孢杆菌属的种
<400> 48
Met Lys Met Tyr Asn Lys Arg Ile Ile Asp Leu Glu Ile Pro Ser Tyr
1 5 10 15
Asn Ala Ile Asn Phe Leu Glu Ile Tyr Arg Leu Pro Leu Val Tyr Leu
20 25 30
Asn Gln Ala Leu Asn Ile Thr Gln His Leu Gln Lys Ser Leu Ser Leu
35 40 45
Gln Thr Leu Asn Leu Glu Phe Asp Gln Leu Asn Asn Leu Ile Ser Glu
50 55 60
Ile Pro Gly Gly Ser Ile Leu Asn Thr Ile Asn Gln Lys Ile Glu Tyr
65 70 75 80
Ser Glu Ile Asn Thr Lys Cys Ala Ile Asn Asn Ile Val Asp Ile Val
85 90 95
Lys Ser Asn Cys Glu Met Phe Glu Asp Asp Phe Ser Leu Gln Ser Lys
100 105 110
Leu Glu Ser Val Ile Ser Asn Val Phe Leu Asn Leu Ala Asn Gln Glu
115 120 125
Glu Asn Pro Tyr Val Phe Tyr His Glu Lys Leu Ser Tyr Asn Thr Ser
130 135 140
Tyr Ser Tyr His Val Ile Phe Ile Ile Asp Asn Met Glu Ser Asn Leu
145 150 155 160
Asn Val Phe Pro Ile Ser Phe Asn Val Asn Val Asp Ser Asn Tyr Glu
165 170 175
Asn Leu Asn Arg Asn Ser Ile Lys Asp Leu His Ser Tyr Lys Ile Lys
180 185 190
Ile Ser Gly Ile Asn Phe Val Ile Ser Lys Asn Ser Glu Leu Ala Glu
195 200 205
Met Val Glu Arg Val Thr Glu Ser Ile Leu
210 215
<210> 49
<211> 360
<212> PRT
<213> 芽孢杆菌属的种
<400> 49
Met Lys Lys Phe Phe Lys Gln Ile Cys Ile Ser Ile Val Ile Leu Leu
1 5 10 15
Met Val Ser Tyr Val Ile Pro Leu His Ile Phe Ala Gln Glu Gln Asn
20 25 30
Ile Gln Gly Gln Gln Ser Asn Glu Lys Tyr Ala Leu Gly Ala Glu Gly
35 40 45
Phe Lys Glu Glu Val Gly His Ser Gly Ser Ser Ile Leu Val Met Asp
50 55 60
Ser Tyr Ala Lys Ile Ile Phe Asn Asn Ser Gln Glu Ile Asp Phe Asn
65 70 75 80
Gly Val Asn Ser Ile Ser Asn Glu Leu Lys Thr Asn Ile Val Ser His
85 90 95
Gln Arg Ala Ala Arg Asn Asn Ala Ala Tyr Trp Leu Glu Asn Ile Arg
100 105 110
Pro Asn Met Leu Gln Thr Val Gln Asp Ile Val Asp Tyr Asn Thr Ile
115 120 125
Phe Gln Val Ser Tyr Ser Thr Met Ala Asp Ala Ile Val Gln Lys Asn
130 135 140
Asn Lys Lys Leu Lys Val Glu Leu Glu Asn Leu Tyr Thr Met Ile Leu
145 150 155 160
Glu Asn Gln Lys Lys Ser Asp Glu Leu Leu Thr Lys Leu Ile Thr Phe
165 170 175
Arg Asp Lys Met Thr Val Asp Thr Gln Asn Phe Lys Ile Asp Val Ala
180 185 190
Asp Val Ser Ala Ile Leu Ala Glu Lys Glu Thr Gly Ile Pro Leu Leu
195 200 205
Lys Gln Gln Ile Asn Ser Tyr Asn Asp Val Ile Lys Lys Asn Asn Asp
210 215 220
Thr Ile Leu Tyr Gly Glu Ile Leu Cys Ala Thr Gly Leu Trp Ile Ile
225 230 235 240
Gly Gly Pro Met Ile Ala Val Ala Leu Asp Asn Ile Asp Asp Ala Glu
245 250 255
Glu Glu Ile Gln Asn Leu Met Ser Gln Ile Ser Ile Ala Gln Glu Glu
260 265 270
Ser Ile Cys Leu Thr Asn Ile Lys Asn Lys Val Ile Tyr Leu Thr Asp
275 280 285
Thr Ile Asp Val Ala Ile Asp Ser Leu Gln Asn Ile Ser Asn Arg Trp
290 295 300
His Thr Ile Gly Ala Lys Tyr Asn Asn Leu Leu Gln Asn Leu Asn Leu
305 310 315 320
Leu Asp Ser Asn Glu Leu Thr Phe Ile Lys Glu Asp Leu Asn Thr Ala
325 330 335
Lys Asp Ser Trp Gln Asp Leu Ser Asp Tyr Ala His Glu Leu Asn Lys
340 345 350
Gly Ile His Glu Ile Glu Lys Asn
355 360
<210> 50
<211> 372
<212> PRT
<213> 芽孢杆菌属的种
<400> 50
Met Lys Phe Lys Ser Thr Lys Leu Ala Ile Cys Thr Met Ala Ala Ala
1 5 10 15
Ala Leu Ser Thr Thr Ile Val Pro Ser Thr Ser Val Phe Ala Ala Glu
20 25 30
Thr Glu Thr Gln Leu Asn Arg Ser Thr Glu Asn Ser Tyr Ser Leu Gln
35 40 45
Lys Ala Asp Ala Glu Ser Tyr Ile Asp Ile Val Gln Asp Arg Met Lys
50 55 60
Gln Arg Asp Arg Glu Ser Lys Cys Thr Gly Lys Pro Ile Asn Met Gln
65 70 75 80
Glu Gln Ile Ile Asp Gly Trp Phe Leu Ser Arg Ile Trp Ile Phe Lys
85 90 95
Asp Gln Asn Asn Ser His Gln Thr Asn Arg Phe Ile Ser Trp Phe Lys
100 105 110
Asp Asn Leu Ala Ser Ser Lys Gly Tyr Glu Ser Ile Ala Thr Gln Met
115 120 125
Gly Leu Asn Ile Glu Ser Leu Asn Asp Met Glu Thr Ser Asn Ile Asn
130 135 140
Tyr Thr Ser Lys Thr Gly Asp Thr Ile Tyr Asn Gly Val Ser Glu Leu
145 150 155 160
Thr Asn Asn Thr Gly Thr Thr Gln Lys Met Lys Thr Asp Ser Phe Gln
165 170 175
Arg Asp Tyr Thr Lys Ser Gln Thr Thr Ser Val Thr Asn Gly Leu Gln
180 185 190
Val Gly Phe Lys Val Thr Ala Lys Gly Val Leu Phe Leu Ala Gly Ala
195 200 205
Asp Phe Glu Thr Ser Val Asn Tyr Asn Leu Ser Thr Thr Thr Ala Gln
210 215 220
Thr Asn Thr Ile Ser Asp Lys Phe Thr Val Pro Ser Gln Glu Val Thr
225 230 235 240
Leu Ala Pro Gly His Lys Ala Ile Val Lys His Asp Leu Arg Lys Met
245 250 255
Val Tyr Ser Gly Thr His Asp Leu Lys Gly Asp Leu Lys Val Ser Phe
260 265 270
Asn Asp Lys Glu Ile Val Gln Lys Phe Ile Tyr Pro Asn Tyr Arg Glu
275 280 285
Ile Asn Leu Ser Asp Ile Arg Lys Thr Met Ile Ala Ile Asp Glu Trp
290 295 300
Asn His Val Lys Pro Ile Asp Phe Tyr Gln Leu Val Gly Ile Lys Asn
305 310 315 320
Gln Ile Lys Asn Gly Asp Thr Ile Tyr Ile Asp Thr Pro Ala Asn Phe
325 330 335
Thr Phe Asn Gly Ala Asn Pro Tyr Tyr Arg Ala Thr Phe Thr Glu Tyr
340 345 350
Asp Ala Asp Gly Lys Pro Ile Lys Thr Lys Lys Leu Ser Glu Asn Tyr
355 360 365
Lys Leu Lys Asn
370
<210> 51
<211> 305
<212> PRT
<213> 芽孢杆菌属的种
<400> 51
Val Ile Gly Ala Ile Ser Ser Ser Phe Ala Phe Thr Ser Pro Gly Ser
1 5 10 15
Val Ser Ala Ala Gln Ile Gly Ser Val Gln Asn Ile Ala Gln Gln Gln
20 25 30
Asn Ala Tyr Ile Ala Asp Trp Arg Glu Pro Phe Glu Ala Ala Tyr Lys
35 40 45
Tyr Met His Asn Leu His Asp Asp Tyr Phe Ile Ser Glu Thr Ala Glu
50 55 60
Tyr Lys Leu Thr Asn Val Tyr Gln Thr Ser Val Glu Gln Ile Gly Ser
65 70 75 80
Pro Thr Ile Thr Asn Ser Ser Ser Leu Phe Val Gly Arg Ser Thr Leu
85 90 95
Thr Asn Asn Thr Asn Gln Asp Gln Thr Leu Thr Thr Asn Glu Phe Ser
100 105 110
Lys Thr Phe Glu Asn Ser Val Thr Asn Ala Thr Thr Asn Gly Phe Asn
115 120 125
Leu Gly Ala Ser Ala Ser Ala Thr Phe Lys Ile Pro Leu Val Gly Glu
130 135 140
Thr Ser Ile Glu Leu Ser Thr Glu Tyr Asn Phe Ser Asp Thr Lys Glu
145 150 155 160
Thr Thr Lys Ser Glu Ser Tyr Thr Tyr Thr Ala Ser Ala Gln Asn Ile
165 170 175
Val Val Pro Ala Asn Ser Ser Val Glu Val Val Val Ser Leu Asn Thr
180 185 190
Ala Lys Ile Asn Gly Asn Val Asn Leu Leu Ser Arg Met Asp Gly Thr
195 200 205
Ile Thr Tyr Asp Arg Ser Leu Asn Gly Gly Tyr Asn Ser Ser Lys Phe
210 215 220
Thr Glu Ser Leu Gly Ser Phe Thr Lys Thr Ala Leu Leu Trp Gly Ser
225 230 235 240
Gln Pro Gly Leu Arg Val Asp Asn Asn Asn Asn Leu Tyr Leu Val Gly
245 250 255
Lys Gly Thr Tyr Ser Ala Glu Tyr Gly Thr Glu Phe Leu Val Ser Val
260 265 270
Arg Pro Val Glu Lys Pro Lys Gly Leu Ala Ala Thr Pro Ser Lys Leu
275 280 285
Asn Asn Glu Gly Tyr Thr Tyr Thr Val Lys Pro Glu Ile Lys Lys Glu
290 295 300
Gln
305
<210> 52
<211> 547
<212> PRT
<213> 芽孢杆菌属的种
<400> 52
Met Glu His Thr Asp Lys Asn Arg Asn Glu Pro Gln Gln Ala Thr Pro
1 5 10 15
Asn Pro Gln Gly Ala Ser Gly Ile Arg Ser Asn Ala Ser Ile Ser Ala
20 25 30
Asp Glu Lys Asp Lys Asn Ala Ile Gln Gln Lys Ser Pro Asn Thr Tyr
35 40 45
Tyr Glu Gly Thr Gln Glu Asn Leu Gly Pro Leu Ala Gln Phe Asp Thr
50 55 60
Trp Val Gln Asp Leu Gly Lys Arg Asn Tyr Lys His Thr Val Ala Met
65 70 75 80
Ala Glu Lys Leu Leu Pro Thr Ile Tyr Lys Asp Leu Asn Arg Gly Asn
85 90 95
Phe Asn Asn Thr Ala Arg Cys Ile Thr Met Leu Ser Thr Ala Leu Ile
100 105 110
Pro Tyr Gly Val Ala Phe Ile Ser Pro Ile Ile Gly Ile Leu Trp Pro
115 120 125
Glu Asn Gly Pro Asn Ile Lys Glu Met Leu Gln Glu Met Glu Asn Lys
130 135 140
Leu Val Arg Ile Met Asp Glu Lys Ile Glu Ala Lys Asp Leu Asp Asp
145 150 155 160
Leu Glu Ala Ala Val Lys Gly Leu Met Val Thr Leu Lys Glu Phe Glu
165 170 175
Asn Ser Leu Gln Gly Asn Ile Gly Gly Glu Asn Tyr Ser Ala Pro Ala
180 185 190
Asp Val Asp Ser Leu Asn Arg Gly Arg Ile Thr Ala Ile Gln Lys Gly
195 200 205
Phe Asn Asp Leu Ile Ser Ala Ala Ser Lys Pro Arg Phe Lys Ile Thr
210 215 220
Glu Leu Pro Ile Tyr Thr Ile Ile Ala Thr Ala His Leu Asn Phe Leu
225 230 235 240
His Thr Val Glu Lys Gln Gly Thr Ser Pro Lys Ile Asn Tyr Thr Glu
245 250 255
Ala Ala Leu Lys Asp Phe Leu Gln Asn Met Lys Lys Asn His Lys Asp
260 265 270
Tyr Ala Asn Tyr Ile Glu Lys Thr Phe Lys Glu Gly Glu Thr Arg Ile
275 280 285
Asp Ser Lys Leu Glu Asp Lys Gln Gln Ile Glu Lys Glu Leu Asp Lys
290 295 300
Val His Glu Gln Leu Ser Ala Leu Gly Pro Lys Pro Lys Asn His Thr
305 310 315 320
His Ile Glu Glu Asn Arg Tyr Ile Asn Lys Lys Glu Glu Leu Tyr Glu
325 330 335
Arg Ala Arg Ser Leu Lys Glu Arg Leu Ser Lys Tyr Asn Glu Leu Met
340 345 350
Tyr Gln Lys Ser Asp Phe Tyr Ser Lys Thr Lys Gly Ser Thr Ala Phe
355 360 365
Gln Ile Ala Ser Thr Gly Lys Thr Thr Ala Asn Pro Ser Trp Val Asn
370 375 380
Thr Glu Gly Ser Trp Val His Asn Gly Phe Trp Tyr Tyr Ile Asp Ala
385 390 395 400
Lys Gly Gln Val Lys Thr Gly Trp Phe Asn Asn Lys Ala Pro Asp Gly
405 410 415
Lys Asp Arg Trp Tyr Tyr Leu Ser Thr Gly Thr Thr Gly Leu Asp Ser
420 425 430
Val Arg Gly Asn Ser Leu Leu Pro Lys Gly Thr Met Leu Thr Gly Trp
435 440 445
Phe His Asp Thr Arg Lys Asp Lys Gln Met Ile Gly Val Asn Thr Lys
450 455 460
Thr Thr Asn Glu Tyr Trp Tyr Tyr Leu Ser Pro Glu Lys Asn Leu Lys
465 470 475 480
Asn Ser Ala Gly Glu Leu Phe Asn Leu Gly Gln Met Met Thr Lys Trp
485 490 495
Val Glu Ile Lys Asp Thr Lys Thr Gly Glu Pro Gln Trp Tyr Tyr Phe
500 505 510
Asn Pro Glu Asp Gly Ser Met Ile His Asp Lys Lys Ala Val Gln Ile
515 520 525
Gly Asn Leu Lys Tyr Asp Phe Asp Ser Asn Gly Val Cys Thr Thr Pro
530 535 540
Asn Gly Tyr
545
<210> 53
<211> 348
<212> PRT
<213> 芽孢杆菌属的种
<400> 53
Val Ser Leu Ser Lys Lys Thr Lys Ile Phe Thr Val Phe Ala Leu Thr
1 5 10 15
Gly Ser Ile Leu Gly Ser Thr Leu Thr Leu Pro Ala Ile Gln Thr Val
20 25 30
Phe Ala Ala Ala Asn Glu Tyr Gln Phe Leu Thr Glu Asn Asp Leu Arg
35 40 45
Asn Gly Val Lys Gln Ala Ile Pro Ser Phe Leu Asp Lys Asn Lys Pro
50 55 60
Tyr Gly Met Tyr Val Glu Tyr Asn Gly Ser Lys Trp Lys Arg Val Ile
65 70 75 80
Asp Pro Lys Glu Leu Asp Gln Trp Tyr Gln Tyr Pro Gln Leu Val Asn
85 90 95
Ala Val Lys Asp Ile Phe Arg Ala Ser Tyr Thr Asn Tyr Val Gly Leu
100 105 110
Lys Asp Leu Thr Ile Glu Asn Gln Asp Ser Lys Ile Thr Ser Leu Arg
115 120 125
Lys Val Gly Asp Pro Glu Leu Ile Gln Leu Gly Thr Ser Glu Leu Val
130 135 140
Asn Thr Asp Ser Glu Thr Lys Gln Asn Leu Thr Ser Ala Pro Phe Ser
145 150 155 160
Lys Ala Phe Thr Ser Thr Ser Thr Val Thr Val Thr Arg Gly Val Asn
165 170 175
Leu Gly Phe Lys Val Gly Ser Lys Ile Ala Ile Pro Leu Val Gly Ala
180 185 190
Asp Ile Ser Thr Glu Phe Ser Arg Asn Thr Asn Trp Ser Thr Asn His
195 200 205
Thr Thr Thr Ser Thr Ser Thr Asp Thr Tyr Thr Ala Pro Ala Gln Thr
210 215 220
Ile Ser Val Pro Pro Arg Thr Lys Ala Ile Val Thr Tyr Val Leu Ser
225 230 235 240
Lys Gln Gln Tyr Glu Gly Glu Ile Ala Leu Lys Gly Asp Leu Thr Gly
245 250 255
Ser Met Phe Leu Tyr Asp Pro Tyr Ile Val Ala Tyr Pro Gly Met Pro
260 265 270
Glu Gln Gln Arg Leu Leu Tyr Thr Ser Thr Leu Tyr Asn Leu Phe Asp
275 280 285
Lys Ser Gly Val Gln Leu Pro Ala Gly Leu Ser Leu Asp Arg Gly Asn
290 295 300
Gln Lys Ile Lys Phe Asn Gly Thr Ala Lys Leu Lys Val Tyr Gly Gly
305 310 315 320
Gln Met His Asp Val Val Leu Lys Ile Val Ser Leu Asp Asp Pro Asn
325 330 335
Lys Val Ile Glu Glu Arg Lys Ile Pro Lys Lys Asn
340 345
<210> 54
<211> 1243
<212> PRT
<213> 芽孢杆菌属的种
<400> 54
Met Asn Gln Asn Tyr Asn Asn Asn Glu Tyr Glu Ile Leu Asp His Gly
1 5 10 15
Asn Arg Ser Tyr Gln Thr Lys Tyr Pro Phe Ala Gln Ala Pro Gly Ser
20 25 30
Glu Cys Gln Lys Pro Asn Ala Lys Asp Gly Ile Tyr Thr Pro Val Gly
35 40 45
Arg Val Asp Thr Val Leu Gln Thr Ile Asp Ile Gly Leu Ser Val Arg
50 55 60
Thr Ala Ile Ser Ile Leu Gln Met Leu Leu Ser Val Ser Phe Pro Ala
65 70 75 80
Leu Gly Arg Ala Ala Gly Leu Ile Asn Ile Ile Phe Gly Phe Leu Trp
85 90 95
Gly Thr Leu Gly Gly Gln Ser Val Trp Glu Ser Phe Met Lys Ala Val
100 105 110
Glu Ser Leu Val Asn Gln Lys Ile Thr Asp Ala Val Arg Ala Lys Ala
115 120 125
Ile Ser Glu Leu Glu Gly Val Gln Asn Ala Leu Glu Leu Tyr Gln Glu
130 135 140
Ala Ala Asp Asp Trp Asn Ala Asn Pro Asp Asp Ala Ser Asn Lys Glu
145 150 155 160
Arg Val Arg Arg Gln Phe Thr Ser Thr Asn Thr Ile Ile Glu Tyr Ala
165 170 175
Met Pro Ser Phe Arg Val Pro Thr Phe Glu Val Pro Leu Leu Thr Val
180 185 190
Tyr Ala Gln Ala Ala Asn Leu His Leu Gln Leu Leu Arg Asp Ala Val
195 200 205
Lys Phe Gly Asn Glu Trp Gly Met Pro Ser Glu Glu Val Glu Asp Leu
210 215 220
Tyr Thr Arg Leu Thr Arg Arg Thr Ala Glu Tyr Thr Asp His Cys Val
225 230 235 240
Thr Ala Tyr Asp Lys Gly Leu Lys Gly Ala Tyr Asp Leu Ala Pro Asn
245 250 255
Pro Thr Asp Tyr Asn Lys Tyr Pro Tyr Leu Asn Pro Tyr Ser Lys Asp
260 265 270
Pro Ile Tyr Gly Lys Tyr Tyr Thr Ala Pro Val Asp Trp Asn Leu Phe
275 280 285
Asn Asp Phe Arg Arg Asp Met Thr Ile Met Val Leu Asp Ile Val Ala
290 295 300
Val Trp Pro Thr Tyr Asn Pro Arg Ile Tyr Thr Asn Pro Asn Gly Val
305 310 315 320
Gln Val Glu Leu Ser Arg Glu Val Tyr Ser Thr Val Tyr Gly Arg Gly
325 330 335
Gly Ser Gly Asn Ser Ser Val Glu Ala Ile Glu Ser Gln Ile Val Arg
340 345 350
Pro Pro His Leu Val Thr Glu Leu Thr Thr Leu Lys Ile Glu Gln Gly
355 360 365
Gly Thr Leu Asp Met Glu Gln Ile Gln Tyr Pro Lys Tyr Met Lys Val
370 375 380
Thr Asn Thr Leu His Tyr Ile Gly Ser Ser Ser Thr Trp Glu Gln Ser
385 390 395 400
Ser Ser Ala Ile Pro Ile Arg Pro Ile Thr Lys Ile His Thr Ile Pro
405 410 415
Ala Asn Asn Ile Gly Asn Leu Ser Leu Ser Gln Leu Glu Val Pro Tyr
420 425 430
Arg Phe Ser Phe Tyr Asn Lys Asp Asp Ala Leu Ile Ser Glu Ile Gly
435 440 445
Ala Glu Phe Pro Pro Asn Asn Val Thr Trp Asn Gly Ile Pro Arg Ala
450 455 460
Glu Asp Ser Asp Gln Asn Ser His His Leu Ser Tyr Val Gly Ala Leu
465 470 475 480
Ser Thr Gln Ser Ser Ala Gly Phe Pro Trp Thr Tyr Pro Thr Glu Leu
485 490 495
Leu Gly Glu Trp Gly Phe Gly Trp Val Ser Asn Ser Leu Thr Pro Glu
500 505 510
Asn Lys Ile Val Asn Asp Lys Ile Thr Gln Ile Pro Ala Val Lys Gly
515 520 525
Tyr Leu Leu Glu Leu Gly Gly Ile Val Ile Lys Gly Pro Gly Ser Thr
530 535 540
Gly Gly Asp Leu Val Gln Leu Ser Ser Asn Gln Ser Gln Val Lys Leu
545 550 555 560
Asn Met Ile Ser Pro Leu Phe Thr Pro Glu Leu Thr Gly His Tyr Ile
565 570 575
Arg Ile Arg Tyr Ala Ser Ser Ala Asp Thr Arg Leu Tyr Ile Ser Trp
580 585 590
Gly Ser Arg Thr Gly Tyr Tyr Asp Ala Lys Ala Thr Tyr Ser Glu Gly
595 600 605
Pro Leu Thr Tyr Lys Thr Phe Gly Tyr Met Asn Ala Gly Trp Leu Tyr
610 615 620
Met Thr Gly Ala Gly Gly Asn Asn Phe Thr Ala Val Ile Lys Asn Gln
625 630 635 640
Gly Asp Thr Pro Ile Ile Ile Asp Lys Ile Glu Phe Ile Pro Phe Thr
645 650 655
Gly Leu Tyr Leu Glu Asp Gln Ala Leu Glu Lys Ala Arg Thr Ala Val
660 665 670
Asn Ala Leu Tyr Thr Ser Asp Ala Lys Ser Ala Leu Lys Leu Asn Ile
675 680 685
Thr Asp Tyr Ala Val Asp Gln Ala Val Asn Leu Val Glu Cys Val Ser
690 695 700
Asp Asp Phe His Ala Lys Glu Lys Met Ile Leu Leu Asp Gln Val Lys
705 710 715 720
Phe Ala Lys Arg Leu Ser Gln Ala Arg Asn Leu Leu His Tyr Gly Asp
725 730 735
Phe Glu Ser Ser Asp Trp Ser Gly Glu Asn Gly Trp Arg Thr Ser Asn
740 745 750
His Val His Val Ala Ser Asp Asn Pro Ile Phe Lys Gly Arg Tyr Leu
755 760 765
His Met Pro Gly Ala Thr Ser Pro Gln Phe Ser Asn Asn Ile Tyr Pro
770 775 780
Thr Tyr Ala Tyr Gln Lys Val Asp Glu Ser Lys Leu Lys Ser Tyr Thr
785 790 795 800
Arg Tyr Leu Val Arg Gly Phe Val Gly Asn Ser Lys Asp Leu Glu Leu
805 810 815
Leu Val Glu Arg Tyr Gly Lys Asp Val His Val Glu Met Asp Val Pro
820 825 830
Asn Asp Ile Gln Tyr Thr Leu Pro Met Asp Glu Cys Gly Gly Phe Asp
835 840 845
Arg Cys Lys Pro Val Ser Asp Gln Ala Arg Ser Pro His Thr Cys Thr
850 855 860
Cys Asn Asp Thr Ala Val Thr His Thr Asp Cys Gln Cys Lys Asp Lys
865 870 875 880
Gly Asn Arg Thr Ser Pro Asn Met Tyr Thr Asn Val Pro Thr Gly Ser
885 890 895
Ala Val Tyr Arg Asn Gly Ser Pro Asp His Lys Ser Cys Gly Cys Lys
900 905 910
Asn Asn Gly Met Tyr Gln Asn Arg Thr His Pro His Lys Ser Cys Gly
915 920 925
Cys Lys Asp Pro His Val Phe Thr Tyr His Ile Asp Thr Gly Cys Val
930 935 940
Asp Gln Glu Glu Asn Leu Gly Leu Trp Phe Ala Leu Lys Ile Ala Ser
945 950 955 960
Glu Asn Gly Val Ala Asn Ile Asp Asn Leu Glu Ile Ile Glu Ala Gln
965 970 975
Pro Leu Thr Gly Glu Ala Leu Ala Arg Val Lys Lys Arg Glu Gln Lys
980 985 990
Trp Lys Gln Glu Met Ala Lys Lys Arg Leu Glu Thr Glu Lys Ala Val
995 1000 1005
Gln Ala Ala Gln Gly Ala Ile Gln Pro Leu Phe Thr Asn Ala Gln
1010 1015 1020
Tyr Asn Arg Leu Gln Phe Glu Thr Leu Phe Pro Gln Ile Val Arg
1025 1030 1035
Ala Glu Lys Leu Val Gln Gln Ile Pro Tyr Val Tyr His Pro Phe
1040 1045 1050
Leu Ser Gly Ala Leu Leu Ala Val Pro Gly Met Asn Phe Asp Ile
1055 1060 1065
Val Gln Gln Leu Ser Ala Leu Phe Asp Arg Ala Arg Gly Leu Tyr
1070 1075 1080
Asp Leu Arg Asn Leu Val Gln Asn Gly Thr Phe Ser Ser Gly Thr
1085 1090 1095
Gly Asn Trp His Val Thr Asp Gly Ile Thr Thr Gln Pro Glu Gly
1100 1105 1110
Asn Thr Ser Val Leu Val Leu Ser Glu Trp Asn His Glu Ala Ser
1115 1120 1125
Gln Gln Leu His Val Asp Pro Asp Arg Gly Tyr Ile Leu Arg Val
1130 1135 1140
Thr Ala Arg Lys Glu Gly Ala Gly Lys Gly Thr Val Thr Met Ser
1145 1150 1155
Asp Cys Ala Asp Tyr Thr Glu Thr Leu Thr Phe Thr Ser Cys Asp
1160 1165 1170
Tyr Asn Thr Val Gly Ile Gln Thr Met Thr Ser Gly Thr Leu Ser
1175 1180 1185
Gly Phe Val Thr Lys Thr Leu Glu Ile Phe Pro Asp Thr Asp Arg
1190 1195 1200
Ile Arg Ile Ala Ile Gly Glu Thr Glu Gly Thr Phe Lys Ile Glu
1205 1210 1215
Ser Val Glu Leu Ile Cys Met Glu Gln Met Glu Asp His Leu Tyr
1220 1225 1230
Asp Met Ala Gly Asn Leu Glu Glu Glu Met
1235 1240
<210> 55
<211> 655
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 55
Met Asn Gln Asn Tyr Asn Asn Asn Glu Tyr Glu Ile Leu Asp His Gly
1 5 10 15
Asn Arg Ser Tyr Gln Thr Lys Tyr Pro Phe Ala Gln Ala Pro Gly Ser
20 25 30
Glu Cys Gln Lys Pro Asn Ala Lys Asp Gly Ile Tyr Thr Pro Val Gly
35 40 45
Arg Val Asp Thr Val Leu Gln Thr Ile Asp Ile Gly Leu Ser Val Arg
50 55 60
Thr Ala Ile Ser Ile Leu Gln Met Leu Leu Ser Val Ser Phe Pro Ala
65 70 75 80
Leu Gly Arg Ala Ala Gly Leu Ile Asn Ile Ile Phe Gly Phe Leu Trp
85 90 95
Gly Thr Leu Gly Gly Gln Ser Val Trp Glu Ser Phe Met Lys Ala Val
100 105 110
Glu Ser Leu Val Asn Gln Lys Ile Thr Asp Ala Val Arg Ala Lys Ala
115 120 125
Ile Ser Glu Leu Glu Gly Val Gln Asn Ala Leu Glu Leu Tyr Gln Glu
130 135 140
Ala Ala Asp Asp Trp Asn Ala Asn Pro Asp Asp Ala Ser Asn Lys Glu
145 150 155 160
Arg Val Arg Arg Gln Phe Thr Ser Thr Asn Thr Ile Ile Glu Tyr Ala
165 170 175
Met Pro Ser Phe Arg Val Pro Thr Phe Glu Val Pro Leu Leu Thr Val
180 185 190
Tyr Ala Gln Ala Ala Asn Leu His Leu Gln Leu Leu Arg Asp Ala Val
195 200 205
Lys Phe Gly Asn Glu Trp Gly Met Pro Ser Glu Glu Val Glu Asp Leu
210 215 220
Tyr Thr Arg Leu Thr Arg Arg Thr Ala Glu Tyr Thr Asp His Cys Val
225 230 235 240
Thr Ala Tyr Asp Lys Gly Leu Lys Gly Ala Tyr Asp Leu Ala Pro Asn
245 250 255
Pro Thr Asp Tyr Asn Lys Tyr Pro Tyr Leu Asn Pro Tyr Ser Lys Asp
260 265 270
Pro Ile Tyr Gly Lys Tyr Tyr Thr Ala Pro Val Asp Trp Asn Leu Phe
275 280 285
Asn Asp Phe Arg Arg Asp Met Thr Ile Met Val Leu Asp Ile Val Ala
290 295 300
Val Trp Pro Thr Tyr Asn Pro Arg Ile Tyr Thr Asn Pro Asn Gly Val
305 310 315 320
Gln Val Glu Leu Ser Arg Glu Val Tyr Ser Thr Val Tyr Gly Arg Gly
325 330 335
Gly Ser Gly Asn Ser Ser Val Glu Ala Ile Glu Ser Gln Ile Val Arg
340 345 350
Pro Pro His Leu Val Thr Glu Leu Thr Thr Leu Lys Ile Glu Gln Gly
355 360 365
Gly Thr Leu Asp Met Glu Gln Ile Gln Tyr Pro Lys Tyr Met Lys Val
370 375 380
Thr Asn Thr Leu His Tyr Ile Gly Ser Ser Ser Thr Trp Glu Gln Ser
385 390 395 400
Ser Ser Ala Ile Pro Ile Arg Pro Ile Thr Lys Ile His Thr Ile Pro
405 410 415
Ala Asn Asn Ile Gly Asn Leu Ser Leu Ser Gln Leu Glu Val Pro Tyr
420 425 430
Arg Phe Ser Phe Tyr Asn Lys Asp Asp Ala Leu Ile Ser Glu Ile Gly
435 440 445
Ala Glu Phe Pro Pro Asn Asn Val Thr Trp Asn Gly Ile Pro Arg Ala
450 455 460
Glu Asp Ser Asp Gln Asn Ser His His Leu Ser Tyr Val Gly Ala Leu
465 470 475 480
Ser Thr Gln Ser Ser Ala Gly Phe Pro Trp Thr Tyr Pro Thr Glu Leu
485 490 495
Leu Gly Glu Trp Gly Phe Gly Trp Val Ser Asn Ser Leu Thr Pro Glu
500 505 510
Asn Lys Ile Val Asn Asp Lys Ile Thr Gln Ile Pro Ala Val Lys Gly
515 520 525
Tyr Leu Leu Glu Leu Gly Gly Ile Val Ile Lys Gly Pro Gly Ser Thr
530 535 540
Gly Gly Asp Leu Val Gln Leu Ser Ser Asn Gln Ser Gln Val Lys Leu
545 550 555 560
Asn Met Ile Ser Pro Leu Phe Thr Pro Glu Leu Thr Gly His Tyr Ile
565 570 575
Arg Ile Arg Tyr Ala Ser Ser Ala Asp Thr Arg Leu Tyr Ile Ser Trp
580 585 590
Gly Ser Arg Thr Gly Tyr Tyr Asp Ala Lys Ala Thr Tyr Ser Glu Gly
595 600 605
Pro Leu Thr Tyr Lys Thr Phe Gly Tyr Met Asn Ala Gly Trp Leu Tyr
610 615 620
Met Thr Gly Ala Gly Gly Asn Asn Phe Thr Ala Val Ile Lys Asn Gln
625 630 635 640
Gly Asp Thr Pro Ile Ile Ile Asp Lys Ile Glu Phe Ile Pro Phe
645 650 655
<210> 56
<211> 282
<212> PRT
<213> 芽孢杆菌属的种
<400> 56
Met Asn Thr Ile Glu Asn Thr Glu Phe Gln Ser Asn Asp Phe Glu Leu
1 5 10 15
Thr Cys Asn His Ser His His Arg Ser Phe Ser Lys Phe Arg His Ile
20 25 30
Thr Leu Thr Ile Pro Ala Glu Glu Gln Tyr Asn Phe Ser Asp Val Tyr
35 40 45
His Val Gln Pro Gln Tyr Leu Pro Gln Ala Leu Arg Leu Thr Asn Ala
50 55 60
Phe Gln Ser Ala Ile Asn Pro Asp Asp Leu Lys Phe Asp Phe Glu Thr
65 70 75 80
Ala Leu Ala Ile Ala Lys Glu Ile Pro Asn Ser Gly Ile Ile Asn Thr
85 90 95
Met Asn Gln Asn Val Val Gln Glu His Leu Gln Ile Ser Val Met Ile
100 105 110
Asp Gln Ile Lys Glu Leu Val Lys Thr Ile Leu Gly Leu Ser Ile Ser
115 120 125
Ser Val Ser Phe Trp Ser Ala Val Ala Gly Ala Ile Thr Asn Thr Phe
130 135 140
Thr Asn Leu Asn Thr Gln Gln Asp Ala Ala Trp Ile Leu Trp Glu Arg
145 150 155 160
Glu Glu Ser Ser Asn Thr Ser Tyr Tyr Tyr Asn Ile Leu Phe Ser Ile
165 170 175
Gln Asn Glu Glu Thr Gly Gly Val Met Ala Val Leu Pro Ile Ser Phe
180 185 190
Glu Ile Ser Val Asn Val Asn Lys Glu Ser Val Leu Phe Phe Thr Ile
195 200 205
Arg Asp Ser Ala Arg Tyr Glu Val Arg Ile Lys Ala Ile Thr Leu Ala
210 215 220
Gln Ala Leu Asp Pro Ile Asn Arg His Leu Arg His Cys Ser Asp Ile
225 230 235 240
Phe Asp Met His Lys Asp Asn Pro Tyr Ile Glu Glu Ile Asn Thr Val
245 250 255
Lys Asn Lys Arg Leu Ile Asp His Ile Ile His Lys Pro Val Arg Ser
260 265 270
Asn Gly Leu Val Leu Pro Asp Ser Cys Glu
275 280
<210> 57
<211> 311
<212> PRT
<213> 芽孢杆菌属的种
<400> 57
Val Asn Asn Lys Leu Leu Val Ser Gly Val Leu Ile Ser Thr Leu Phe
1 5 10 15
Gly Gly Ala Ile Asn Asn Val His Ala Asp Thr Ile Lys Asn Asp Ser
20 25 30
Asn Phe Leu Asn Thr Lys Leu Gly Gln Val Gly Gln Glu Gly Ala Leu
35 40 45
Gln Asp Ile Asp Asn Ile Leu Asn Gln Leu Val Asp Ser Ile Asn Lys
50 55 60
Asn Ser Pro Leu Met Thr His Ile Ala Leu Ala Asp Asn Gly Ile Val
65 70 75 80
Asn Tyr Ser Gly Ile Asn Ile Asn Gln Ser Asn Val Thr Asn Val Ile
85 90 95
Pro Leu Phe Leu Gly Asn Asn Thr Phe Ser Asn Thr Ser Ser Glu Glu
100 105 110
Gln Ser Tyr Asn Thr Ser Ile Phe Ser Gln Ala Ile Thr Tyr Thr Lys
115 120 125
Ser Thr Ser Ile Gln Asn Gly Phe Lys Thr Gly Leu Lys Val Gly Gly
130 135 140
Lys Ala Gly Ile Pro Phe Val Ala Glu Gly Ala Val Glu Thr Ser Leu
145 150 155 160
Glu Tyr Asn Phe Ser Ser Thr Gly Thr Ser Thr Ser Ser Glu Thr Asn
165 170 175
Thr Ile Thr Ala Pro Ser Gln Pro Val Lys Val Pro Ala Gly Lys Thr
180 185 190
Tyr Lys Val Glu Val Tyr Phe Glu Lys Lys Ser Thr Ser Gly Thr Val
195 200 205
Asp Leu Phe Ala Asp Val Asn Gln Gly Val Arg Gly Asn Arg Leu His
210 215 220
Pro Asp Phe Ala Lys Leu Ser His Phe Leu Tyr Arg Ala Glu Asp Lys
225 230 235 240
His Gly Val Ile Gln Thr Pro Asn Asp Pro Asp Ser Ile Arg Ile Lys
245 250 255
Gly Ser Gly Asn Phe Arg Ile Glu Tyr Gly Thr Asn Leu Ile Val Lys
260 265 270
Thr Tyr Asp Ile Ser Ser Lys Pro Asn Leu Lys Phe Arg Ser Leu Ile
275 280 285
Asn Ser Glu Leu Glu Ser Asp Ile Leu Val Asn Glu Gln Ile Ile Pro
290 295 300
Leu Lys Ser Pro Asn Lys Lys
305 310
<210> 58
<211> 307
<212> PRT
<213> 芽孢杆菌属的种
<400> 58
Met Thr Ile Ile Asp Met Gly Ala Val Ala Glu Glu Tyr Met Arg Trp
1 5 10 15
Asn Phe Ser Arg Asp Pro Asp Val Val Pro Gly Ser Pro Arg Tyr Phe
20 25 30
Asn Leu Phe Asp Gly Asp Ile Tyr Asp Asn Arg Ala Glu Pro Thr Gln
35 40 45
Asp Thr Phe Glu Val Thr Pro Ile Leu Ala Lys Thr Ala Ser Gln Thr
50 55 60
Leu Lys Asn Gly Thr Ser Val Pro Gln Gln Gln Thr Val Lys Phe Thr
65 70 75 80
Glu Lys Lys Ala Thr Thr Thr Lys Thr Ser Thr Thr Ser Gly Phe Lys
85 90 95
Asn Ser Lys Thr Thr Lys Thr Gly Thr Lys Leu Asn Ala Gly Ile Lys
100 105 110
Leu Asp Trp Phe Ser Ala Gly Gly Glu Phe Ser Thr Glu Val Ser Val
115 120 125
Thr Gly Glu Tyr Asn His Ser Thr Thr Gln Glu His Thr Thr Thr Thr
130 135 140
Glu Ser Thr Trp Glu Val Thr Gln Pro Val Thr Val Pro Ala Tyr Ser
145 150 155 160
Thr Val Ile Ala Lys Leu Tyr Ile Tyr Asp Ala Ser Phe Ser Val Thr
165 170 175
Cys Asn Leu Asp Gly Lys Ile Arg Gly Thr Ala Thr Ser Pro Ile Pro
180 185 190
Thr Tyr Asn Asp Tyr Met Asn Met Gln Tyr Thr Lys Lys Ser Thr Asn
195 200 205
Arg Thr Val Thr Arg Trp Gln Tyr Ala Gly Asp Met Tyr Asn Ala Asn
210 215 220
Trp Pro Gly Lys Pro Gln Ser Phe Ile Gly Arg Gly Ser Ala Gly Asp
225 230 235 240
Tyr Asn Ser Leu Tyr Tyr Lys Gly Ala Gly Val Ala Arg Val Ala Ser
245 250 255
Gly Ile Tyr Thr Glu Val Glu Phe Ile Glu Thr Arg Ala Ser Gly Glu
260 265 270
Val Val Ser Trp Lys Tyr Gly Tyr Ile Pro Asn Pro Asp Gly Ser Leu
275 280 285
Leu Val Val Pro Gln Gly Ser Arg Gly Ile Glu Val Leu Gln Gly Ser
290 295 300
Gln Ile Tyr
305
<210> 59
<211> 575
<212> PRT
<213> 芽孢杆菌属的种
<400> 59
Met Lys Tyr Lys Asp Arg Arg His Thr Lys His Lys Tyr Lys Gln Val
1 5 10 15
Leu Leu Ala Ala Val Thr Thr Thr Thr Val Gly Ile Ser Thr Leu Gly
20 25 30
Ser Thr Thr Ser Ile Phe Ala Glu Glu Lys Glu Lys Asn Ala Val Gln
35 40 45
Gln Gln Asn Thr Val Gln Gln Lys Ile Ser Asp Lys Tyr Tyr Glu Arg
50 55 60
Gly Ile Glu Met Glu Pro Leu Ala Gln Phe Asp Asn Trp Thr Gln Asn
65 70 75 80
Leu Ala Lys Asn Thr Gly Ala Ala Asn Tyr Lys Asn Thr Leu Ser Leu
85 90 95
Ala Glu Lys Thr Leu Pro Ile Leu Tyr Lys Asp Leu Lys Lys Gly Asn
100 105 110
Phe Ser Asn Thr Ala Arg Ser Leu Leu Met Phe Gly Thr Ala Leu Ile
115 120 125
Pro Tyr Gly Gly Ala Phe Ile Ser Pro Val Leu Gly Leu Leu Trp Pro
130 135 140
Glu Asn Val Glu Asp Gln Lys Asn Gln Leu Gln Ala Leu Arg Lys Glu
145 150 155 160
Leu Thr Thr Leu Met Asp Gln Lys Ile Glu Asp Tyr Asp Val Ala Thr
165 170 175
Leu Lys Gln Gln Thr Lys Ala Leu Ile Asn Glu Leu Arg Phe Phe Glu
180 185 190
Asp Ser Val Asn Gly Lys Tyr Ala Lys Ser Pro Ala Val Gly Gly Thr
195 200 205
Ile Gln Glu Thr Arg Arg Ile Leu Ala Ile Thr Ile Asn Ser Lys Phe
210 215 220
Lys Asp Leu Ile Glu Leu Cys Glu Lys Glu Lys Gln Gln Val Ala Glu
225 230 235 240
Leu Pro Thr Tyr Thr Ile Val Ala Ala Ala His Leu Glu Phe Leu Arg
245 250 255
Phe Ile Glu Val Asn Gly Lys Asp Pro Asn Lys Ile His Ile Asp Asp
260 265 270
Glu Thr Leu Lys Ser Asn Phe Val Asn Gln Ile Pro Glu Phe Thr Asp
275 280 285
Arg Tyr Ile Thr His Ile Gln Lys Thr Tyr Thr Glu Gly Glu Gln Gln
290 295 300
Phe Leu Asp Lys Met Asn Asp Ile Thr Thr His Ser Val Ser Glu Ser
305 310 315 320
Glu Asn Leu Arg Tyr Leu Lys Ser Leu Asp Asn Pro Arg Asp Glu Met
325 330 335
Gly Tyr Thr Gln Asp Lys Ile Asp Lys Ile Lys Gln Asn Ile Lys Asp
340 345 350
Tyr Glu Lys Tyr Ser Ser Ala Lys His Thr Phe Met Leu Asn Thr Ile
355 360 365
Ser Ser Leu Ala Tyr Lys Thr Met Leu Leu Thr Gly Trp Lys Gln Glu
370 375 380
Gly Asn Lys Tyr Tyr Tyr Leu Asp Lys Asn Asn Met Lys Arg Thr Gly
385 390 395 400
Trp Phe Ile Asp Gly Asp Gln Lys Tyr Tyr Leu Ser Pro Glu Lys Thr
405 410 415
Gly Glu Phe Glu Lys Gly Glu Met Val Thr Gly Leu Ile Asn Ile Lys
420 425 430
Asn Thr Ser Asp Ser Pro Ala Leu Thr Tyr Tyr Phe Asn Pro Glu Lys
435 440 445
Gly Lys Lys Gln Gly Thr Leu Met Met Gly Trp Ile Lys Asn Gly Asp
450 455 460
Asn Trp Tyr Tyr Ala Ser Pro Arg Asn Gly Thr Lys Asn Trp Asp Asn
465 470 475 480
Lys Ser Phe Asp Arg Gly Val Leu Val Thr Gly Trp Val Lys Ile Asp
485 490 495
Asn Glu Tyr Tyr Tyr Phe Ser Pro Ala Asp Gly Thr Lys Asn Asp Asp
500 505 510
Gly Lys Glu Phe Lys Lys Gly Glu Met Val Thr Gly Trp Met Asn Val
515 520 525
Asn Gly Ala Trp Tyr Tyr Leu Asn Thr Glu Lys Thr Tyr Gly Leu Ile
530 535 540
Gly His Met Leu His Asp Gln Thr Ala Ser Val Lys Asn Lys Asn Gly
545 550 555 560
Glu Tyr Lys Gln His Arg Phe Asn Glu Asn Gly Thr Leu Ala Trp
565 570 575
<210> 60
<211> 631
<212> PRT
<213> 芽孢杆菌属的种
<400> 60
Met Asn Lys Asn Ser Asn Asn Asn Glu Phe Thr Asn Asn Asn Gly Met
1 5 10 15
Val Ser Gln Pro Lys His Pro Phe Val Lys Glu Pro Gly Ser Glu Leu
20 25 30
Gln Asn Met Asn Thr Val Gly Ala Pro Ile Ala Leu Ser Ser Thr Thr
35 40 45
Thr Arg Asp Gly Val Ile Ile Ala Thr Gly Ile Gly Trp Ala Ile Leu
50 55 60
Gly Phe Val Pro Val Val Gly Pro Gly Leu Ser Ala Ala Ala Gly Leu
65 70 75 80
Leu Asn Val Ile Val Pro Phe Leu Trp Pro Glu Asp Asp Ala Gly Ser
85 90 95
Ser Gln Ile Ser Trp Glu Gln Leu Met Asp Ser Val Glu Glu Leu Val
100 105 110
Asp Glu Arg Ile Leu Thr Ser Lys Arg Ser Asp Ala Thr Ala Arg Leu
115 120 125
Glu Gly Ile Lys Ala Leu Val Asn Glu Tyr Thr Gln Ala Ile Asp Asp
130 135 140
Leu Lys Ala Asp Pro Asn Asn Glu Leu Lys Lys Asp Arg Val Arg Asp
145 150 155 160
Lys Phe Asp Asp Val Glu Asp Tyr Val Lys Val Ser Met Pro Phe Phe
165 170 175
Arg Ala Gln Gly Phe Glu Val Pro Met Leu Ala Met Tyr Ala Gln Ala
180 185 190
Ala Asn Ile His Leu Leu Leu Leu Arg Asp Val Val Gln His Gly Ala
195 200 205
Ser Trp Gly Phe Gln Gln Tyr Glu Ile Asp Arg Tyr Tyr Asn His Thr
210 215 220
Gly Leu Thr Gly Ser Pro Gly Met Leu Gln Leu Leu Ala Gln Tyr Thr
225 230 235 240
Ala His Cys Val Arg Trp Tyr Asn Thr Gly Leu Glu Asn Arg Arg Ala
245 250 255
Thr Asn Asp Trp Asn Lys Phe Asn Asp Phe Arg Arg Asp Met Thr Leu
260 265 270
Met Val Leu Asp Ile Ile Ser Leu Trp Pro Thr Tyr Asp Pro Thr His
275 280 285
Tyr Ala Val Pro Thr Lys Thr Gln Leu Thr Arg Thr Val Tyr Thr Pro
290 295 300
Trp Ile Gly His Leu Arg Asn Arg Trp Gln Gly Tyr Pro Asp Ala Leu
305 310 315 320
Ser Leu Ser Val Val Glu Asn Ser Ile Val Pro Ala Pro His Leu Phe
325 330 335
Thr Trp Leu Arg Glu Val Trp Phe Tyr Phe Thr Gly Gly Gly Gly Pro
340 345 350
Glu Ala Glu Thr Leu Phe Thr Gly Phe Arg Pro Phe Phe Gln Thr Thr
355 360 365
Leu Ser Asp Asn Leu Leu Thr Gly Glu Leu Gln Gly Asn Tyr Gly Thr
370 375 380
Glu Pro Arg Gln Asp Ile Thr Ile Pro Ser Pro Gln Ser Gly Asp Asp
385 390 395 400
Ile Trp Lys Val His Thr Ser Ser Thr Leu Met Ala Glu Gly Pro Glu
405 410 415
His Arg Arg Leu Lys Gly Trp Trp Phe Leu Phe Thr Asn Ser Pro Thr
420 425 430
Gln Gln Ala His Thr Lys Thr Ala Tyr Trp Gln Gln Leu Ile Tyr Thr
435 440 445
His Leu Gly Leu Pro Cys Lys Asn Ser Cys Gly Asp Glu Asn Pro Asn
450 455 460
Leu Ser Asn Pro Cys Asp Asn Ser Ser Leu Tyr Ser His Arg Leu Ser
465 470 475 480
Asn Phe Arg Gly Asn Phe Asp Pro Thr Tyr Cys Ser Tyr Gly Trp Thr
485 490 495
His Val Ser Ala Asp His Asn Asn Leu Ile Asp Ala Gln Lys Ile Thr
500 505 510
Gln Ile Pro Ala Val Lys Ala Ser Ser Ile Ser Gly Gly Ala Arg Val
515 520 525
Val Lys Gly Pro Gly Ser Thr Gly Gly Asp Leu Val Glu Leu Asp Phe
530 535 540
Ser Thr Ala Ser Ser Leu Arg Leu Lys Val Thr Leu Ala Asp Thr Thr
545 550 555 560
Lys Ser Tyr Gln Ile Arg Thr Arg Ser Ala Tyr Gly Ser Val Ser Lys
565 570 575
Ser Ala Asp Thr Leu Thr Tyr Ser Asp Phe Ser Tyr Ala Asn Ser Thr
580 585 590
Ile Ile Pro Pro Arg Asp Ser Ser Glu Tyr Thr Tyr Ile Met Glu Leu
595 600 605
Asp Pro Ser Asp Phe Gly Gly Leu Glu Gly Lys Phe Phe Ile Asp Lys
610 615 620
Ile Glu Phe Ile Pro Ile Gly
625 630
<210> 61
<211> 630
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 61
Met Asn Lys Asn Ser Asn Asn Asn Glu Phe Thr Asn Asn Asn Gly Met
1 5 10 15
Val Ser Gln Pro Lys His Pro Phe Val Lys Glu Pro Gly Ser Glu Leu
20 25 30
Gln Asn Met Asn Thr Val Gly Ala Pro Ile Ala Leu Ser Ser Thr Thr
35 40 45
Thr Arg Asp Gly Val Ile Ile Ala Thr Gly Ile Gly Trp Ala Ile Leu
50 55 60
Gly Phe Val Pro Val Val Gly Pro Gly Leu Ser Ala Ala Ala Gly Leu
65 70 75 80
Leu Asn Val Ile Val Pro Phe Leu Trp Pro Glu Asp Asp Ala Gly Ser
85 90 95
Ser Gln Ile Ser Trp Glu Gln Leu Met Asp Ser Val Glu Glu Leu Val
100 105 110
Asp Glu Arg Ile Leu Thr Ser Lys Arg Ser Asp Ala Thr Ala Arg Leu
115 120 125
Glu Gly Ile Lys Ala Leu Val Asn Glu Tyr Thr Gln Ala Ile Asp Asp
130 135 140
Leu Lys Ala Asp Pro Asn Asn Glu Leu Lys Lys Asp Arg Val Arg Asp
145 150 155 160
Lys Phe Asp Asp Val Glu Asp Tyr Val Lys Val Ser Met Pro Phe Phe
165 170 175
Arg Ala Gln Gly Phe Glu Val Pro Met Leu Ala Met Tyr Ala Gln Ala
180 185 190
Ala Asn Ile His Leu Leu Leu Leu Arg Asp Val Val Gln His Gly Ala
195 200 205
Ser Trp Gly Phe Gln Gln Tyr Glu Ile Asp Arg Tyr Tyr Asn His Thr
210 215 220
Gly Leu Thr Gly Ser Pro Gly Met Leu Gln Leu Leu Ala Gln Tyr Thr
225 230 235 240
Ala His Cys Val Arg Trp Tyr Asn Thr Gly Leu Glu Asn Arg Arg Ala
245 250 255
Thr Asn Asp Trp Asn Lys Phe Asn Asp Phe Arg Arg Asp Met Thr Leu
260 265 270
Met Val Leu Asp Ile Ile Ser Leu Trp Pro Thr Tyr Asp Pro Thr His
275 280 285
Tyr Ala Val Pro Thr Lys Thr Gln Leu Thr Arg Thr Val Tyr Thr Pro
290 295 300
Trp Ile Gly His Leu Arg Asn Arg Trp Gln Gly Tyr Pro Asp Ala Leu
305 310 315 320
Ser Leu Ser Val Val Glu Asn Ser Ile Val Pro Ala Pro His Leu Phe
325 330 335
Thr Trp Leu Arg Glu Val Trp Phe Tyr Phe Thr Gly Gly Gly Gly Pro
340 345 350
Glu Ala Glu Thr Leu Phe Thr Gly Phe Arg Pro Phe Phe Gln Thr Thr
355 360 365
Leu Ser Asp Asn Leu Leu Thr Gly Glu Leu Gln Gly Asn Tyr Gly Thr
370 375 380
Glu Pro Arg Gln Asp Ile Thr Ile Pro Ser Pro Gln Ser Gly Asp Asp
385 390 395 400
Ile Trp Lys Val His Thr Ser Ser Thr Leu Met Ala Glu Gly Pro Glu
405 410 415
His Arg Arg Leu Lys Gly Trp Trp Phe Leu Phe Thr Asn Ser Pro Thr
420 425 430
Gln Gln Ala His Thr Lys Thr Ala Tyr Trp Gln Gln Leu Ile Tyr Thr
435 440 445
His Leu Gly Leu Pro Cys Lys Asn Ser Cys Gly Asp Glu Asn Pro Asn
450 455 460
Leu Ser Asn Pro Cys Asp Asn Ser Ser Leu Tyr Ser His Arg Leu Ser
465 470 475 480
Asn Phe Arg Gly Asn Phe Asp Pro Thr Tyr Cys Ser Tyr Gly Trp Thr
485 490 495
His Val Ser Ala Asp His Asn Asn Leu Ile Asp Ala Gln Lys Ile Thr
500 505 510
Gln Ile Pro Ala Val Lys Ala Ser Ser Ile Ser Gly Gly Ala Arg Val
515 520 525
Val Lys Gly Pro Gly Ser Thr Gly Gly Asp Leu Val Glu Leu Asp Phe
530 535 540
Ser Thr Ala Ser Ser Leu Arg Leu Lys Val Thr Leu Ala Asp Thr Thr
545 550 555 560
Lys Ser Tyr Gln Ile Arg Thr Arg Ser Ala Tyr Gly Ser Val Ser Lys
565 570 575
Ser Ala Asp Thr Leu Thr Tyr Ser Asp Phe Ser Tyr Ala Asn Ser Thr
580 585 590
Ile Ile Pro Pro Arg Asp Ser Ser Glu Tyr Thr Tyr Ile Met Glu Leu
595 600 605
Asp Pro Ser Asp Phe Gly Gly Leu Glu Gly Lys Phe Phe Ile Asp Lys
610 615 620
Ile Glu Phe Ile Pro Ile
625 630
<210> 62
<211> 250
<212> PRT
<213> 芽孢杆菌属的种
<400> 62
Val Asn Pro Phe Arg Tyr Lys Thr Arg Leu Met Lys Gly Glu Cys Lys
1 5 10 15
Met Thr Ser Ile Tyr Leu Lys Lys Asn Glu Cys Ile Arg Asn Leu Arg
20 25 30
Asn Pro Pro Ile Arg Arg Ile Thr Leu Lys Val Pro Cys Lys Asn Lys
35 40 45
Gly Asn Leu Lys Asn Ile Phe Gln Val Glu Pro Gln Tyr Ile Asn Gln
50 55 60
Ala Leu Thr Ala Ser Asn Ala Phe Gln Gly Ala Ile Asp Ser Asn Leu
65 70 75 80
His Phe Asn Tyr Glu Arg Ala Tyr Gln Ile Gly Val Asn Met Pro Gly
85 90 95
Arg Asp Tyr Asp Pro Ser Met Trp Gly Ile Asn Gln Pro Val Ile Gln
100 105 110
Glu Thr Leu Gln Val Ser Ser Met Ile Asn Gln Leu Lys Ser Ile Leu
115 120 125
Asn Arg Phe Leu Tyr Phe Ile Asp Ile Ser Asp Glu Pro Phe Trp Asn
130 135 140
Glu Val Glu Ala Val Ile Val Asn Thr Phe Thr Asn Leu Tyr Thr Gln
145 150 155 160
Gln Asn Ser Ala Trp Ile Phe Tyr His Ser Ser Thr Ser Thr Ser Thr
165 170 175
Ser Tyr Tyr Tyr Asn Ile Met Phe Ala Leu Gln Asp Ser Asn Tyr Arg
180 185 190
Thr Met Ile Val Ile Pro Ile Ala Phe Asp Ile Thr Val Arg Leu Glu
195 200 205
Lys Gln Lys Val Leu Phe Ile Thr Leu Lys Asp Arg Ala Glu Tyr Arg
210 215 220
Val Thr Leu Lys Ser Ile Trp Val Arg Leu Ile Leu Gln Lys Asn Gln
225 230 235 240
Asp Lys Gln Ile Val Asp Cys Phe Asn Ser
245 250
<210> 63
<211> 388
<212> PRT
<213> 芽孢杆菌属的种
<400> 63
Met His Ser Ile Thr Arg Tyr Lys Lys Met Gly Ile Ile Val Pro Leu
1 5 10 15
Val Phe Val Leu Gly Thr Ser Leu Thr Val Val Asn Lys Pro Ile His
20 25 30
Ala Ala Ala Ala Thr Asn Asn Ile Thr Ile Ser Gln Ala Ser Ser Asp
35 40 45
Thr Phe Lys Glu Val Asn Ile Ser Thr Leu Arg Gly Asp Leu Tyr Glu
50 55 60
Arg Phe Val Thr Leu Phe Glu Gly Asn Pro Arg Leu Leu Gly Ile Asn
65 70 75 80
Ser Asp Gly Lys Lys Leu Thr Asp Lys Asp Lys Gln Gln Ile Arg Ser
85 90 95
Phe Ala Gln Ala Ile Arg Asp Asp Asn Phe Lys Asn Ile Asp Asp Ile
100 105 110
Asp Lys Leu Thr Lys Gly Gln Phe Lys Lys Gly Thr Phe Ile Ala Pro
115 120 125
Phe Met Asn Trp Ser Pro Gln Tyr Ser Asn Leu Thr Thr Asn Tyr Asp
130 135 140
Leu Lys Ala Thr Pro Gly Asp Val Lys His Glu Ser Val Glu Val Tyr
145 150 155 160
Lys Tyr Lys Asn Pro Lys Asn Ser Val Glu Gln Thr Phe Lys Thr Asp
165 170 175
Glu Lys Glu Val Glu Ile Gly His Thr Phe Thr Glu Thr His Ala Thr
180 185 190
Gly Gly Lys Phe Gly Tyr Ser Asn Thr Thr Thr Phe Lys Val Gly Val
195 200 205
Pro Leu Asn Gln Ala Thr Thr Thr His Thr Phe Thr Phe Glu Ala Asn
210 215 220
Tyr Asn Tyr Thr Asn Thr His Thr Lys Thr Asp Lys Glu Thr Lys Arg
225 230 235 240
Tyr Pro Ser Gln Thr Val Lys Cys Glu Pro Gly Tyr Ile Thr Gln Leu
245 250 255
Thr Ile Asp Lys Ser Thr Ala Thr Tyr Phe Gly Glu Leu Glu Phe Asn
260 265 270
Ile Gly Ser Ser Ala Lys Glu Leu Met Asn Gln Ile Asn Gln Asn Ile
275 280 285
Lys Asp Ile Asn Ala Phe Ser Lys Asn Lys Leu Ser Val Ile Gln Pro
290 295 300
Asp Glu Glu Glu Leu Met Tyr Thr Leu Tyr Lys Tyr Ser Gly Tyr Asp
305 310 315 320
Ile Pro Ser Tyr Val Thr Leu Asp Asp Glu His Lys Thr Val Ser Phe
325 330 335
Gly Lys Ile Lys Ser Thr Tyr Thr Gly Val Thr Gly Thr Ala Ser Asp
340 345 350
Met Ile Leu Glu Leu Thr Pro Ile Asp Ser Lys Asp Thr Thr Lys Lys
355 360 365
Ser Ile Lys Met Pro Phe Lys Glu Tyr Gln Gln Lys Arg Lys Asn Asn
370 375 380
Glu Pro Ile Ile
385
<210> 64
<211> 461
<212> PRT
<213> 链霉菌属的种
<400> 64
Val Gly Ala Asp Leu Glu Arg Ala Pro Ala Arg Arg Ala Pro Asp Pro
1 5 10 15
Pro Arg Arg His Thr Gly Arg Gly Val Arg Arg Ala Pro Gly Gly His
20 25 30
Arg Gly Arg Gly Ser Arg Pro Ala Pro Arg Arg Arg Gly Arg Pro Gln
35 40 45
Arg His Pro Pro Leu His Arg Asp Asp Gly Arg Arg Arg Pro Gln Gly
50 55 60
Pro Pro Val Arg Arg Gly Gly Gly Gly Leu Gly Arg Arg Pro Arg Arg
65 70 75 80
Gly Ala Arg Pro Ala Pro Pro Arg Arg His Pro Gly Leu Gly Ala Ala
85 90 95
Ala Arg Pro Pro Arg Asp Gly Ala Pro Gly Asp Asp Arg Arg Arg Asp
100 105 110
Arg Ala Gly Arg Gly Gly Arg Arg Arg Ala Arg Gly Ala Ala Val Ala
115 120 125
Gly Pro Pro Ala Pro Pro Gln Gly Pro His Leu Pro Asp Pro Arg Arg
130 135 140
Arg Pro Ala Val Ala Gly Gly Pro Val Val Ala Gly Gly Gly Arg Gly
145 150 155 160
Ala Arg Pro Gly Gly Gly Val His Arg His Arg Ala Arg Pro His Arg
165 170 175
Glu Gln His Pro Gly Pro Pro Arg Arg Gly Glu Glu Gly Pro Glu Ala
180 185 190
Ala Gln Gly Ala Ala Gly Arg Glu Ala Gly Glu Val Arg Glu Ala Gly
195 200 205
Arg Arg Phe Pro Asp Arg Gly Pro Pro Leu Gly Val Pro Gln Gly Arg
210 215 220
Arg Ser Leu Arg Pro Glu Arg Glu Ala Ala Pro Arg Pro Arg Leu Ser
225 230 235 240
Arg Pro His Gly Gly Arg Pro Ala Pro Gly Pro Pro Arg Leu Gly Ile
245 250 255
Leu Thr Pro Pro Asp His Arg Asp His Pro Gly Gly Ala Leu Ala Ala
260 265 270
Ser Phe Arg Thr Val Val Glu Val Ala Pro Asp His Leu Asp Gln Ala
275 280 285
Arg Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro Ala Thr Val
290 295 300
Asn Phe Asp Phe Glu Ala Ile His Arg Ala Ala Ser Ala Val Pro Asp
305 310 315 320
Ser Asp Val Val Lys Met Val Arg Gly Trp Gly Leu Gln Glu Tyr Ala
325 330 335
Pro Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val Arg Gln Ala
340 345 350
Leu Pro Pro Glu Cys Ala Glu Ala Gly Phe Trp Ala Thr Val Glu Arg
355 360 365
Glu Leu Val Gln Ala Phe Thr Gly Leu Ala Ala Gln Glu Gly Gln Pro
370 375 380
Gly Leu Ser Phe Tyr Glu Glu Ser Gly Asp Arg Thr Arg Phe Tyr Arg
385 390 395 400
Asp Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Asp His Met Tyr
405 410 415
Ala Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro Lys Ala Glu
420 425 430
Thr Leu Ala Leu Asp Leu Thr Asp Ile Val Pro Phe Ala Val Arg Leu
435 440 445
Asn Ala Ile Val Val Arg Gln Ala Leu Ser Val Ala Val
450 455 460
<210> 65
<211> 362
<212> PRT
<213> 芽孢杆菌属的种
<400> 65
Met Ala Ile Glu Asn Asn Arg Tyr Tyr Tyr Ile Lys Tyr Phe Asn Ser
1 5 10 15
Thr Asn Trp Val Trp Asp Ile Gln Gly Glu Ser Gln Asp Asp Gly Gly
20 25 30
Asn Val Ile Leu Tyr Asp Lys Lys Asn Ser Asn Asn Gln Gln Phe Val
35 40 45
Met Phe Leu Leu Asp Thr Gly Tyr Ala Ile Val Asn Lys Asn Ser Gly
50 55 60
Arg Val Ile Gly Thr Glu Phe Pro Gly Ser Ser Gly Pro Arg Tyr Tyr
65 70 75 80
Phe Gly Gly Asn Lys Leu Leu Tyr Gln Glu Ser Trp Lys Gly Asn Pro
85 90 95
Ile Gln Gln Phe Leu Phe Lys Glu Gln Thr Ile Pro Gly Phe Thr Asn
100 105 110
Val Tyr Leu Ile Gln Asn Gln Gly Leu Gly Leu Ala Ala Ser Tyr Ala
115 120 125
Glu Gln Ala Gly Ile Pro Phe Ala Phe Val Asp Leu Asp Pro Pro Asn
130 135 140
Pro Asn Asp Gly Gln Gln Gly Phe Gln Leu Ile Glu Gly Asp Ala Ile
145 150 155 160
Asp Leu Pro Ser Leu Pro Pro Leu Gln Ser Gly Arg Pro Pro Val Pro
165 170 175
Asp Tyr Ile Phe Ser Val Tyr Asp Val Leu Pro Gln Thr Ser Ile Ser
180 185 190
Tyr Leu Val Gly Ala Thr Leu Ile Pro Cys Ile Met Val Asn Asp Asn
195 200 205
Gln Arg Ser Pro Ser Thr Lys Ile Gln Asp Ser Pro Tyr Tyr Leu Leu
210 215 220
Thr Lys Glu Val Tyr Trp Asp Gln Glu Tyr Ser Lys Val Val Thr Pro
225 230 235 240
Ser Asn Pro Ile Lys Thr Thr Tyr Lys Ser Gly Ile Ser Asn Ile Asp
245 250 255
Gln Thr Ser Met Ile Asp Thr Leu Lys Ile Ser Ile Gly Ala Asp Phe
260 265 270
Gly Phe Lys Tyr Asp Ala Phe Ser Gly Ser Leu Lys Thr Ser Ile Glu
275 280 285
Lys Thr Leu Gln Thr Thr Val Ser Ser Thr Gln Thr Ser Leu Gln Glu
290 295 300
Lys Thr Val Glu Glu Thr Leu Thr Ala Asn Pro Asn Phe Ser Lys Thr
305 310 315 320
Gly Ile Thr Glu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Tyr Arg Ala
325 330 335
Asp Gly Ser Leu Val Val Glu Pro Trp Ile Val Lys Asp Asp Asn Ile
340 345 350
Ser Val Thr Gln Asp Phe Phe Gln Asn Val
355 360
<210> 66
<211> 819
<212> PRT
<213> 芽孢杆菌属的种
<400> 66
Met Lys Asn Lys Lys Gln Lys Val Leu His Met Thr Val Ala Ala Ser
1 5 10 15
Val Leu Ala Thr Thr Tyr Val Pro Ala Ala Asn Phe Ala Phe Ala Gln
20 25 30
Glu Lys Gln Glu Gln Gly Leu Lys Glu Thr Gln Val Thr Pro Phe Asn
35 40 45
Glu Leu Lys Glu Lys Ile Thr Glu Asn Pro Asn Leu Asp Asn Ser Arg
50 55 60
Met Trp Asn Pro Tyr Lys Gly Val Asp Tyr Val Lys Phe Met Asn Ala
65 70 75 80
Leu Asn Ser Asn Val Trp Lys Pro Val Leu Thr Asp Ala Val Lys Asn
85 90 95
Glu Gly Glu Asn Val Val Pro Val Leu Ile Lys Gly Ala Ile Val Met
100 105 110
Gly Leu Ser Leu Ala Pro Pro Pro Leu Pro Met Leu Gly Thr Val Val
115 120 125
Asp Leu Phe Gln Phe Thr Asp Gly Lys Thr Asp Asp Ala Trp Ser Arg
130 135 140
Thr Val Glu Tyr Val Asn Lys Thr Ile Asp Ser Lys Ile Glu Gln Tyr
145 150 155 160
His Ser Tyr Leu Met Ser Ser Glu Leu Val Ala Leu Gly Ser Ser Leu
165 170 175
Gly Glu Tyr Lys Arg Val Leu Glu Ile Tyr Glu Thr Asn Lys Leu Thr
180 185 190
Arg Val Glu Asp Pro Ala Ala Gly Val Arg Glu Gln Phe Arg Ile Ser
195 200 205
Asn Asp Lys Ala Lys Ala Phe Ile Ala Lys Leu Gln His Pro Gln Asn
210 215 220
Gln Gly Asp Thr Arg Tyr Asn Val Leu Thr Leu Pro Leu Phe Ala Gln
225 230 235 240
Ala Ala Asn Leu His Leu Ala Leu Leu Arg Asp Ala Ile Val Cys Asp
245 250 255
Trp Gly Ile Asp Ser Asn Gln Leu Gln Gly Tyr Lys Asp Glu Leu Lys
260 265 270
Lys Ser Ile Ile Asp Tyr Thr Asn Tyr Ile Asn Lys Thr Tyr Lys Glu
275 280 285
Gly Leu Glu Gln Glu Lys Asn Lys Ala Ala Asp Tyr Ser Asp Pro Glu
290 295 300
Ser Tyr Ile Gln Ala Gly Lys Ser Asp Val Pro Val Leu Arg Gln Trp
305 310 315 320
Phe Pro Glu Val Met Lys Trp Asn Arg Val Ala Asn Tyr Glu Arg Gln
325 330 335
Met Ser Ala Ser Val Leu Asp Leu Ala Ala Val Leu Pro Thr Tyr Asp
340 345 350
Pro Asp Asn Tyr Ser Lys Gln Pro Ile Val Lys Gln Ser Arg Pro Ile
355 360 365
Phe Ala Pro Ile Ile Gly Ile Pro Gly Gly Leu Lys Ser Lys Asp Ser
370 375 380
Ala Gly Thr Phe Gly Ser Tyr Val Phe Asp Val Lys Asn Phe Asp Gln
385 390 395 400
Ile Asn Thr Glu Leu Ala Lys Ser Pro Tyr Asp Tyr Leu Ala Tyr Gly
405 410 415
Gly Ala Leu Asn Gln Ile Gly Ile Arg Thr Ser Gly Tyr Ile Tyr Gly
420 425 430
Ser Ser Thr Asn Thr Thr Tyr Tyr Thr Asn Met Thr His Gly Ser Thr
435 440 445
Ala Gly Asn Gln Thr Asn Ile Ser Ile Asn Pro Asn Asn Pro Ile Thr
450 455 460
Ser Ile Ser Gly Thr Ala Gly Thr Lys Pro Tyr Thr Leu Ser Phe Gln
465 470 475 480
Phe Ala Asp Gly Gly Thr Thr Gln Thr Leu Gly Ser Asn Val Pro Asn
485 490 495
Val Asn Cys Tyr Ala Pro Phe Lys Phe Glu Tyr Pro Gly Tyr Gln Leu
500 505 510
Ser Ala Val Arg Val Phe Gly Thr Ser Ala Ser Thr Gly Asp Ala Asp
515 520 525
Ala Met Val Phe Ala Phe Arg Ser Thr Glu Ile Gly Asp Thr His Gln
530 535 540
Leu Met Ala Asn Val Pro Thr Gln Ile Pro Ala Glu Ile Gly Tyr Gly
545 550 555 560
Gln Glu Asn Thr Leu Asn Glu Val Ile Asn Gly Gln Asn Ala Ile Glu
565 570 575
Met Asn Asn Gly Asn Thr Met Lys Tyr Ser Ile Asn Ser Pro Lys Thr
580 585 590
Gln Gln Tyr Lys Val Arg Tyr Arg Val Ala Ala Asn Glu Glu Ser Ser
595 600 605
Val Arg Leu Gln Asn Met Asp Thr Asn Ile Ser Lys Thr Thr Asp Thr
610 615 620
Ser Asn Thr Ser Arg Asn Thr Val Lys Gly Glu Tyr Gly Tyr Tyr Lys
625 630 635 640
Leu Val Glu Gly Pro Thr Ile Thr Leu Asn Lys Gly Ile Asn Thr Val
645 650 655
Thr Leu Gln Gly Thr Lys Gly Lys Phe Ala Leu Asp Tyr Ile Glu Val
660 665 670
Glu Pro Ile Val Gln Asp Glu Arg Val Tyr Phe Glu Asp Phe Ile Gly
675 680 685
Asp Leu Lys Ser Ser Trp Tyr Gly Ser Asn Ala Gly Phe Ala Pro Gly
690 695 700
Tyr Gly Thr Val His Glu Asn Gly Glu Leu Tyr Thr Phe Ile Asn Glu
705 710 715 720
Lys Val Glu Lys Tyr Ser Gln Tyr Thr Met Arg Val Lys Leu Lys Leu
725 730 735
Asn Ser Asn Asp Pro Asn Val Arg Glu Asn Val Arg Leu Phe Thr Asp
740 745 750
Asn Ala Lys Gly Glu Arg Ile Thr Lys Glu Val Thr Leu Lys Gly Gly
755 760 765
Ala Asn Tyr Gln Glu Phe Lys Leu Glu Phe Met Thr Asn Asp Asn Leu
770 775 780
Asp Lys Thr His Ile Gly Val Gln Lys Ala Ser Gly Ser Asn Thr Val
785 790 795 800
Phe Val Lys Asp Val Glu Val Ile Gly Thr Pro Asn Asp Asn Ala Thr
805 810 815
Pro Tyr Lys
<210> 67
<211> 337
<212> PRT
<213> 乳球菌属的种
<400> 67
Met Lys Lys Asn Arg Lys Ile Ile Asn Thr Cys Val Val Val Leu Val
1 5 10 15
Phe Gly Ala Ser Phe Ser Pro Ile Ala Arg Ala Asp Gly Met Leu Lys
20 25 30
Asn Ile Ser Asp Val Asn Gln Asp Arg Ile Val Asp Val Ser Glu Thr
35 40 45
Met Glu Phe Ile Ala Gln Asn Tyr Tyr Glu Ile His Met Lys Asp Lys
50 55 60
Leu Leu Leu Cys Trp Val Ser Gly Ser Asp Gly Tyr Met Ile Arg Lys
65 70 75 80
Leu Asp Lys Ile Thr Pro Gly Glu Thr Trp Ser Glu Ile Glu Gln Ser
85 90 95
Ser Ile Gln Thr Ser Asn Leu Gln Leu Ser Pro Glu His Leu Glu Asn
100 105 110
Val Gln Val Ser Leu Leu Asp Asn Thr Leu Asn Ile Asn Gln Ala Met
115 120 125
Ser Ser Thr Ser Tyr Cys Lys Ala Val Thr Asn Thr Ser Thr Thr Gln
130 135 140
Thr Asn Gln Gly Val Lys Ala Ser Ala Glu Ala Lys Leu Leu Pro Ile
145 150 155 160
Pro Leu Ile Met Pro Asp Gly Ile Lys Leu Thr Ala Asp Val Asn Ser
165 170 175
Ser Thr Asp Thr Asn Ile Ser Lys Thr Thr Thr Glu Thr Ile Ile Val
180 185 190
Pro Pro Gln Thr Val Ile Val Lys Pro His His Lys Val Lys Val Thr
195 200 205
Ala Glu Phe Tyr Thr Asn Lys Tyr Ser Gly Asp Ile Ile Tyr Asn Thr
210 215 220
Lys Val Asn Lys Ser Ser Thr Gly Leu Lys Val Asn Ala Glu Tyr Tyr
225 230 235 240
Val Pro Phe Asn Pro Phe Ile Gly Trp Leu Thr Pro Asn Arg His Asp
245 250 255
Thr Ile Thr Leu Asp Asn Tyr Glu Met Tyr Arg Gly Asp Asp Ser Ser
260 265 270
Ile Glu Lys Arg Leu Ile Gln Ala Gly Val Lys Phe Asn Ala Asp Gly
275 280 285
Ser Met Gln Ala Leu Cys Lys Thr His Phe Asp Ser Thr Ser Gly Ser
290 295 300
Tyr Ala Lys Ile Ser Val Ile Asp Val Thr Asn Leu Ser Cys Pro Leu
305 310 315 320
Ile Leu Lys Glu Gln Val Tyr Lys Asn Phe Gln Thr Asn Asn Ser Asn
325 330 335
Ser
<210> 68
<211> 518
<212> PRT
<213> 芽孢杆菌属的种
<400> 68
Met Lys Tyr Asn Glu Arg Gln Lys Ala Lys Arg His Tyr Lys Lys Thr
1 5 10 15
Met Phe Ala Val Ala Ala Ala Leu Ser Ile Gly Val Thr Gly Val Gly
20 25 30
Gly Ala Met Ala Val Pro Thr Ala Thr Tyr Ala Ala Thr Asn Ala Gln
35 40 45
Lys Gly Thr Asp Ile Ile Gln Ile Met Ser Asp Gly Ser Ile Lys Ile
50 55 60
Val Asn Asp Gln Leu Asp Lys Gln Ser Pro Ala Lys Ile Leu Thr Asn
65 70 75 80
Leu Gly Gly Ser Ala Phe Ile Asn Leu Val Lys Gly Ala Gln Asn Asn
85 90 95
Gly Met Thr Thr Val Asn Asn Thr Ala Arg Asp Ile Val Met Ala Gly
100 105 110
Thr Ser Gly Ile Pro Tyr Gly Gly Ala Ile Ile Ser Ser Leu Ile Gly
115 120 125
Leu Leu Trp Pro Gln Asp Gly Val Asn Gln Met Lys Gln Met Met Glu
130 135 140
Glu Met Ala Lys Met Met Asp Gln Lys Ile Glu Asp Phe Asp Lys Ala
145 150 155 160
Gly Leu Asn Gln Gln Ile Lys Asp Ala Gln Gly Ala Leu Arg Glu Met
165 170 175
Glu Leu Val Leu Tyr Gly Asp Gly Ser Gly Ser Thr Arg Ser Leu Pro
180 185 190
Gly Thr Ala Ser Glu Lys Ala Gly Leu Ala His Ser Lys Leu Met Thr
195 200 205
Ala Leu Ser Ala Ala Gln Lys Glu Gly Tyr Asp Arg Thr Glu Leu Pro
210 215 220
Ala Tyr Thr Ala Ala Ala Thr Ala His Leu Leu Phe Leu Ser Phe Met
225 230 235 240
Val Glu Asn Gly Glu Ser Pro Leu Phe Lys Ile Asp Ser Asp Ser Met
245 250 255
Asn Arg Thr Tyr Lys Ile Tyr Leu Lys Glu Ala Ile Ser Asp Tyr Lys
260 265 270
Thr His Ile Asn Arg Thr Val Gly Asp Gly Arg Asn Ser Leu Tyr Gln
275 280 285
Ala Asn Tyr Arg Ile Gln Glu Ile Asn Lys Glu Ile Ala Arg Leu Ala
290 295 300
Lys Arg Gln Glu Thr Ser Gly Pro Lys Gly Phe Leu Gln Leu Gln Asp
305 310 315 320
Lys Val Asn Glu Gln Leu Gly Tyr Lys Asn Asp Val Leu Lys Asp Lys
325 330 335
Ala Lys Phe Asp Asn Tyr Val Asn Ser Thr Val Asn Asn Ala Ala Phe
340 345 350
Asn Glu Ala Leu Asn Phe Ala Ile Asp Thr Ala Thr Lys Asn Lys Ile
355 360 365
Ser Ile Thr Asp Pro Arg Ser Gln Ile Ala Asn Gly Val Tyr Val Ile
370 375 380
Ala Ser Gly Leu Asp Asn His Met Val Leu Asp Asn Asp Asn Gly Thr
385 390 395 400
His His Asn Val Ala Thr Tyr Glu Glu His Asp Gly Lys Asn Gln Arg
405 410 415
Trp Leu Ile Ser Tyr Glu Lys Asn Lys Gln Ala Tyr Lys Ile Gln Asn
420 425 430
Leu Gly Asp Asn Gly Pro Val Leu Ala Thr Gly Ser Ser Ala Asn Tyr
435 440 445
Asn Val Phe Gly Tyr Glu Phe Ser Gly Ser Asp Asp Gln Tyr Trp Ile
450 455 460
Pro Glu Ser Val Gly Asp Gly Tyr Tyr Ile Leu Lys Asn Lys Ala Phe
465 470 475 480
Pro Asp Arg Val Leu Asp Val Ser Asn Gly Lys Ile Gly Asn Gly Thr
485 490 495
Asn Val Thr Ile Tyr Pro Arg Asp Ala Lys Asn Pro Ala Asn Gln Lys
500 505 510
Phe Lys Phe Ile Ala Gln
515
<210> 69
<211> 426
<212> PRT
<213> 芽孢杆菌属的种
<400> 69
Met Lys Pro Asn Ser Pro Glu Asn Gln Pro Pro Ser Pro Lys Trp Tyr
1 5 10 15
Lys Gly Asp Tyr Asp Lys Tyr Arg Asp Gly Tyr Ala Tyr Asn Glu Asn
20 25 30
Ile Gly Tyr Glu Asn Lys Asn Trp Met Ser Val Leu Arg Val Thr Thr
35 40 45
Lys Ile Ser Gln Leu Ser Ile Pro Gly Thr His Gly Ser Thr Ala Leu
50 55 60
His Gly Val Thr Gly Gly Ser Arg Trp Ile Ile Asn Gln Arg Met Ser
65 70 75 80
Ile Thr Thr Gln Leu Asn Ser Gly Ile Arg Tyr Leu Asp Ile Arg Ala
85 90 95
Arg Arg Thr Gly Thr Ala Phe Ala Met His His Gly Ala Val Tyr Gln
100 105 110
Lys Lys Met Phe Gly Asp Ile Leu Asn Glu Val Thr Ala Phe Leu Arg
115 120 125
Gln His Ser Gly Glu Thr Val Leu Met Arg Leu Lys Glu Glu His Thr
130 135 140
Pro Glu Pro Gly Ser Gln Ser Phe Glu Glu Ile Leu Lys Gly Tyr Trp
145 150 155 160
Asp Asn Pro Ser Tyr Lys Asn Tyr Phe Trp Gln Pro Ala Gly Ser Gly
165 170 175
Asn Gly Ser Gly Val Asn Ala Asn Pro Glu Leu Lys Asp Val Arg Gly
180 185 190
Lys Ile Val Leu Leu Gln Gln Phe Ser Val Ser Lys Ala Tyr Gly Ile
195 200 205
Asn Tyr Ser Asn Leu Glu Ile Gln Asp His Asp Asn Val Arg Phe Ser
210 215 220
Met Asp Gly Met Tyr Ala Lys Trp Thr Ala Val Lys Asn His Leu Ile
225 230 235 240
Lys Gly Asn Ser Asp Gln Asn Lys Ile Tyr Leu Asn His Leu Ser Gly
245 250 255
Asn Gly Gly Pro Asn Gly Gly Cys Asp Pro Gln Phe Val Ala Ser Gly
260 265 270
Tyr Lys Gly Gly Gly Asp Asp Glu Pro Leu Met Gly Ile Gly Ser Pro
275 280 285
Ser Gln Tyr Pro Asp Phe Pro Thr Glu Asn Tyr Arg Leu Tyr Tyr Gly
290 295 300
Gly Met Asn Ile Met Thr Thr Gln Tyr Ile Pro Arg Ser Gly Leu Glu
305 310 315 320
His Thr Gly Ile Val Ala Ala Asp Phe Pro Gly Lys Gly Leu Val Gly
325 330 335
Ser Ile Ile Gly Arg Asn Tyr Arg His Ile Asn Asn Glu Gln Arg Glu
340 345 350
Ile Ser Ile Gly Leu Val Ile Pro Glu Asn Ser Val Ala Lys Val Thr
355 360 365
Val Tyr Ser Thr Thr Gly Ala Thr Ile Gly Lys Gln Val Met Ala Asn
370 375 380
Thr Asn Leu Val Glu Leu Asn Val Thr Phe Ser Phe His Ile Pro Asp
385 390 395 400
Gly Val Arg Tyr Asp Lys Tyr Thr Leu Glu Ile Phe Pro Leu Asn Thr
405 410 415
Arg Lys Arg Glu Leu Leu Ala Glu Leu Phe
420 425
<210> 70
<211> 343
<212> PRT
<213> 芽孢杆菌属的种
<400> 70
Met Lys Lys Tyr Lys Lys Leu Leu Met Val Ala Pro Leu Ala Cys Met
1 5 10 15
Leu Gly Thr Gly Leu Phe Thr Leu Pro Thr Ala Ser His Ala Asp Ala
20 25 30
Ala Pro Val Ala Ser Val Val Asn Asp Gln Phe Gly Gln Gly Ser Thr
35 40 45
Tyr Ser Thr Glu Asn Ile Ser Asn Val Leu Phe Val Arg Leu Arg Ala
50 55 60
Gly Ile Glu Ala Ser Pro Glu Leu Arg Gln Lys Phe Lys Ile Ala Asp
65 70 75 80
Asp Glu Lys Ile Gln Phe Glu Thr Gly Glu Gly Gly Gly Thr Ile Gly
85 90 95
Gly Asp Lys Val Asn Ala Ile Asn Tyr Gly Leu Arg Asp Gln Leu Ser
100 105 110
Lys Val Thr Ala Ser Ser Thr Gly Thr Asn Ile Thr Val Asn Pro Thr
115 120 125
Thr Val Thr Glu Val Glu Ala Gly Lys Ile Glu Leu Leu Ser Tyr Arg
130 135 140
Asn Asp Thr Pro Ile Thr Gln Lys Gln Phe Thr Thr Glu Lys Gln Val
145 150 155 160
Lys Arg Val Asp Thr Val Thr Ala Thr Asn Gln Tyr Gly Phe Lys Leu
165 170 175
Gly Phe Glu Ser Thr Thr Ser Phe Glu Val Asn Ala Leu Val Ala Lys
180 185 190
Ala Glu Gln Ser Phe Lys Ala Thr Ala Glu Phe Asn Phe Ser His Thr
195 200 205
Asp Thr Asn Thr Val Thr Asn Glu Glu Thr Val Thr Phe Lys Ala Gln
210 215 220
Glu Val Gln Ala Ala Val Gly Gly Thr Thr Asn Tyr Phe Thr Arg Val
225 230 235 240
Gly Lys Ala Glu Phe Ser Gly Ser Phe Gln Thr Asp Ala Ala Leu Thr
245 250 255
Asp Leu Lys Leu Lys Leu Pro Ile Val Lys Gln Gly Asn Asn Glu Thr
260 265 270
Val Val His Thr Glu Glu Val Thr Leu Thr Ser Gln Asp Ile Tyr Thr
275 280 285
Ile Phe Lys Asn Thr Pro Tyr Ile Pro Leu Pro Ala Tyr Leu Ser Phe
290 295 300
Asp Asp Asn Gly Lys Lys Val Leu Val Lys Asn Val Thr Phe Asn Tyr
305 310 315 320
Thr Gly Glu Met Gly Tyr Asn Thr Ala Ala Gln Ala Glu Phe Ile Pro
325 330 335
Phe Asp Gln Asp Asn Pro Lys
340
<210> 71
<211> 366
<212> PRT
<213> 芽孢杆菌属的种
<400> 71
Met Thr Asn Gly Asn Lys Asn Asn Glu Asp Ser Leu Gln Asn Lys Ile
1 5 10 15
Lys Ser Ile Val Asn Phe Pro Asp Phe Asp Ile Tyr Lys Ile Arg Thr
20 25 30
Thr Tyr Asn Arg Tyr Val Asp Val Ser Gly Asp Pro Asp Asn Asn Leu
35 40 45
Ala Pro Ile Val Gln His Asp Gly His Asn Gly Asn Asn Gln Lys Phe
50 55 60
Leu Ile Tyr Thr Leu Asp Asn Gly Tyr Ser Val Ile Ala Ala Arg His
65 70 75 80
Ser Gly Lys Val Val Asp Val Arg Tyr Leu Asn Leu Pro Phe Pro Asn
85 90 95
Pro Asn Leu Asp His Leu Ile Gln His Asp Phe Lys Asn Gly Asp Asn
100 105 110
Gln Lys Phe Arg Leu Ala Thr Glu Gly Thr Ile Glu Ile Lys His Asp
115 120 125
Gly Gln Val Trp Asp Val Ala Gly Asn Gly Asn Asn Ser Leu Ile Ile
130 135 140
Pro Phe Arg Tyr His Gly Ala Ser Asn Gln Lys Phe Arg Leu Glu Lys
145 150 155 160
Ser Gly Ser Val Ser Ile Lys Thr Pro Ser Ile Val Asn Leu Pro Pro
165 170 175
Ala Pro Asp Phe Lys Thr Asn Asp Leu Asn Glu Gln Leu Pro Lys Glu
180 185 190
Thr Thr Pro Val Val Thr His Val Thr Tyr Leu Pro Tyr Phe Met Val
195 200 205
Lys Asp Arg Tyr Tyr Asn Ala Leu Gln Lys Ile Gln Asn Ser Pro Tyr
210 215 220
Tyr Val Leu Val Arg Arg Gln Tyr Trp Asp Lys Ile Thr Gln Arg Ile
225 230 235 240
Leu Ala Pro Ser Glu Thr Tyr Ser Tyr Lys Glu Thr Thr Gly Val Ser
245 250 255
Arg Thr Asp Gln Thr Ser Met Thr Glu Thr Thr Glu Ile Ser Ile Gly
260 265 270
Ala Asp Leu Gly Phe Met Phe Lys Gly Phe Ser Leu Gly Leu Ser Thr
275 280 285
Ser Ile Thr Lys Ser Leu Ser Val Thr Lys Ser Thr Ser Asn Thr Glu
290 295 300
Ser Thr Glu Ile Thr Lys Asp Val Ser Tyr Thr Asn Pro Phe Thr Tyr
305 310 315 320
Gln Ile Ala Tyr Ala Lys Tyr Met Leu Ile Asn Glu Tyr Tyr Val Thr
325 330 335
Arg Ala Asn Gly Glu Met Ile Thr Thr Gly Asp Ala Ala Tyr Trp Arg
340 345 350
Val Pro Asp Pro Glu Gln Thr Val Ser Arg Ile Ile Pro Arg
355 360 365
<210> 72
<211> 304
<212> PRT
<213> 芽孢杆菌属的种
<400> 72
Val Ile Val Asp Val Glu Arg Asp Leu Ile Asp Asn Asn Glu Trp Tyr
1 5 10 15
Ala Arg Val Thr Tyr Asn Ala Asn Pro Ser Thr Phe Arg Asn Gln Ser
20 25 30
Val Tyr Asp Met Asn Val Ser Asp Leu Asp Val Val Pro Ile Thr Thr
35 40 45
Glu Phe Glu Ser Thr Pro Gln Leu Thr Asn Ser Ala Ser Gln Ile Val
50 55 60
Arg Asn Tyr Thr Ser Lys Asp Gln Ser Gln Thr Val Leu Phe Ser Glu
65 70 75 80
Lys Ser Ile Glu Thr Phe Ser Arg Ser Thr Thr Glu Gly Tyr Lys Ile
85 90 95
Gly Ser Ser Ile Lys Ser Thr Thr Ser Phe Lys Val Lys Val Gly Phe
100 105 110
Leu Val Ser Gly Glu Ile Ser Gln Ser Ile Glu Val Ala Val Thr Gly
115 120 125
Glu Tyr Asn His Ser Ser Thr Glu Thr Thr Thr Thr Thr Asn Glu Lys
130 135 140
Leu Trp Gln Val Thr Gln Pro Val Ile Ile Pro Pro Tyr Thr Gln Val
145 150 155 160
Thr Ala Thr Leu Gln Ile Phe Ser Gly Pro Phe Val Val Pro Ala Lys
165 170 175
Thr Lys Ala Thr Ile Gln Gly Lys Gly Thr Asn Asn Gly Ala Tyr Asn
180 185 190
Phe Ala Ser Val Ile Thr Tyr Thr Asp Tyr Gly Gly Lys Thr Trp Thr
195 200 205
Asp Arg Asn Arg Ala Gln Ala Leu Tyr Thr Asp Arg Gly Gln Trp Pro
210 215 220
Gly Tyr Lys Pro Ile Tyr Val Gly Gly Ser Ser Ser Thr Asp Pro Thr
225 230 235 240
Gly Leu Leu Arg Leu Glu Gly Glu Ala Arg Ile Asn Ala Gln Val Gly
245 250 255
Leu Tyr Ala Val Thr Glu Phe Lys Glu Ser Pro Leu Pro Gly Tyr Ala
260 265 270
Asp Ile Gly Ser Asn Arg Thr Tyr Tyr Ala Pro Asn Ile Ile Leu Gly
275 280 285
Asp Gly Arg Ile Ile Pro Val Pro Gly Tyr Gln Arg Tyr Leu Gln Arg
290 295 300
<210> 73
<211> 985
<212> PRT
<213> 芽孢杆菌属的种
<400> 73
Val Leu Leu Thr Leu Tyr Leu Phe Ser Lys Gly Ile Leu Ile Asn Asn
1 5 10 15
Leu Ser Gly Val Met Asn Val Leu Ser Asn Ser Met Val Pro Tyr Asn
20 25 30
Val Leu Arg Asn Ile Lys Met Pro Thr Ile Ser Gly Thr Arg Trp Asp
35 40 45
Lys Glu Met Phe Ser Ser Ala Leu Asp Asn Thr Ser Val Leu Leu Asn
50 55 60
Leu Ile Glu Lys Gly Ile Asn Glu Gly Asp Asp Leu Leu Gly Leu Phe
65 70 75 80
Ser Phe Ile Gly Leu Thr Ala Leu Glu Ala Ile Pro Ile Val Gly Gly
85 90 95
Ala Leu Ser Lys Leu Ile Ser Ile Ile Phe Phe Ser Ser Lys Pro Ser
100 105 110
Ile Ser Tyr Gln Lys Ile Trp Glu Glu Leu Val Lys Ala Ile Asn Gln
115 120 125
Ile Val Asp Lys Lys Ile Glu Glu Ala Leu Val Ser Glu Leu Ile Gln
130 135 140
Glu Leu Ser Gly Phe Ala Asn Val Leu Glu Glu Tyr Arg Asp Ala Tyr
145 150 155 160
Asp Leu Tyr Asn Gly Lys Arg Val Phe Glu Ile Pro Asp Asn Leu Thr
165 170 175
Pro Glu Glu Tyr Leu Val Thr Val Phe Thr Ala Ala Asn Leu Gln Phe
180 185 190
Leu Gln Arg Ile Pro Arg Phe Gln Asn Pro Lys Phe Asp Val Val Phe
195 200 205
Leu Pro Phe Phe Val His Val Ala Glu Met His Ile Leu Leu Ile Arg
210 215 220
Asp Ala Ala Ile His Gly Leu Glu Trp Gly Met Asp Glu Lys Met Asn
225 230 235 240
Gln Lys Phe Lys Lys Asp Leu Lys Asn Cys Ile Glu Glu Tyr Thr Thr
245 250 255
Tyr Leu Leu Lys Ile Tyr Lys Lys Gly Leu Lys Glu Ala Ser Glu Arg
260 265 270
Glu Leu Lys Asp Lys Asp Phe Pro Thr Thr Arg Asp Lys Lys His Tyr
275 280 285
Ile Asn Thr Val Arg Trp Asn Val Ile Asn Gln Tyr Lys Arg Gly Met
290 295 300
Thr Leu Thr Val Phe Asp Phe Ala Tyr Arg Trp Lys Tyr Tyr Gln Glu
305 310 315 320
Asp Tyr Arg Asn Asn Ile Thr Leu Asn Pro Ile Arg Thr Ile Tyr Ser
325 330 335
Asp Ile Ala Gly Ser Val Tyr Pro Tyr Glu Lys Thr Thr Asn Glu Ile
340 345 350
Asp Ile Thr Ile Lys Asp Gln Asn Val Lys Tyr Arg Gly Leu Leu Lys
355 360 365
Lys Leu Gln Val Tyr His Ala Glu Arg Ile Asp Ser Ile Gln Ser Thr
370 375 380
Tyr Leu Arg Asn His Glu Ile Ile Asn Ser Asn Lys Ile Gly Gly Thr
385 390 395 400
Gly Gly Arg Ile Ser Ser Leu Asp Leu Glu Asp His Ile Asn Asn Pro
405 410 415
Leu Ile Gln Val Asn Met Trp Ser Glu Leu Val Pro Ile Ser Leu Gly
420 425 430
Phe Lys Phe Tyr Asn Glu Lys Glu Glu Lys Ile Gly Gly Gly Ser Trp
435 440 445
Trp Pro Asn Pro His Lys Phe Gly Ala Tyr His Phe Val Gly Asn Lys
450 455 460
Val Ser Ser Ile Ile Gly Phe Gly Lys Asn Glu Thr Gly Gly Phe Asn
465 470 475 480
Ser Leu Asp Ala Met Ala Val Gly Phe Lys Leu Asp Asp Tyr Asp Ser
485 490 495
Lys Asn Arg Leu Phe Gly Ile Asn Lys Asn Gly Glu Pro Val Thr Lys
500 505 510
Val Ile Asp Ala Gly Asn Phe Tyr Lys Asp Asn Phe Lys Ser Asn Ile
515 520 525
His Lys Ala Asp Glu Pro Ile Phe Gly Asp Gly Ile Leu Gln Phe Thr
530 535 540
Asn Tyr Leu Ser His Gln Asn Gln Asp Ser Ser Val Met Tyr Gln Ile
545 550 555 560
His Val Glu Ile Glu Gly Thr Tyr Gln Leu His Ala Ile Ile Gly Ala
565 570 575
Lys Lys Gln Arg Glu Lys Leu Ser Ile Lys Val Ile Leu Asn Asp Glu
580 585 590
Gln Gln Lys Asn Leu Ile Thr Asp Tyr Phe Asp Ser Ser Asn Thr Trp
595 600 605
Thr Gly Met Ser Leu Asn Asn Glu Trp Val Tyr Lys Arg Val Leu Ile
610 615 620
Gly Asn Phe Gln Leu Lys Arg Gly Ile Asn Asn Ile Asn Ile His Asn
625 630 635 640
Ser Ile Leu Gln Asp Ser Ala Asn Ile Lys Thr Trp Asn Leu Gly Ser
645 650 655
Ile Glu Leu Thr Met Asp Thr Asn Ser Ile Lys Asp Pro Asp Val Thr
660 665 670
Thr Leu Tyr Asp Lys Asp Asn Tyr Ala Gly Thr Lys Arg Phe Ile Phe
675 680 685
Glu Asp Thr Arg Arg Leu Ile Asp Phe Asn Asp Arg Thr Ser Ser Ile
690 695 700
Lys Val Gly Ser Tyr Val Pro Gly Leu Arg Phe Tyr Glu His Tyr Asp
705 710 715 720
Tyr Gly Gly Gly Phe Ile Asp Leu Gly Ser Gly Glu Lys Leu Ser Leu
725 730 735
Lys Asn His Arg Leu Asn Lys Lys Leu Ser Ser Ala Lys Phe Ala Asn
740 745 750
Leu Val Leu Tyr Lys Glu Asp Asp Tyr Lys Gly Glu Lys Thr Leu Ile
755 760 765
Phe His Asp Ile Pro Tyr Leu Thr Asp Phe Asn Asp Lys Thr Ser Ser
770 775 780
Leu Val Val Ser Ser Asn Val Ser Gly Ile Val Arg Leu Phe Glu His
785 790 795 800
Ser Phe Tyr Asn Gly Glu Tyr Ile Asp Val Ser Gly Gly Gln Lys Ile
805 810 815
Ser Leu Lys Gly His Asn Leu Asn Arg Arg Ile Ser Ser Ile Lys Phe
820 825 830
Leu Lys Glu His Glu Val Phe Asn Gly Leu Tyr Glu Ile Val Thr Ala
835 840 845
Ile Asn Asn Lys Ser Val Val Asp Trp Ser Lys Asp Lys Asn Ser Asn
850 855 860
Val His Leu Trp Glu Arg Arg Asn Gln Asp Asn Gln Lys Trp Tyr Phe
865 870 875 880
Lys Tyr Asp Ser Glu Lys Gln Ala Phe Gln Ile Ile Ser Lys Ala Asp
885 890 895
Glu Tyr Glu Val Leu Ala Trp Asn Asp Phe Lys Asn Ser Met Asn Val
900 905 910
Phe Ala Thr Tyr Asn Ser Lys Lys Asp Glu His Tyr Trp Ile Leu Asp
915 920 925
Tyr Leu Gly Glu Gly Asn Tyr Leu Ile Arg Asn Lys Lys Lys Gln Asn
930 935 940
Leu Val Leu Asp Val Asp Gly His Lys Thr Ala Asn Gly Thr Asn Ile
945 950 955 960
Lys Val Asn Glu Gln His Ala Leu Asp Asn Pro Ser Ile Glu Ala Gln
965 970 975
Lys Phe Lys Ile Asn Leu Val Asp Asn
980 985
<210> 74
<211> 340
<212> PRT
<213> 短芽孢杆菌属的种
<400> 74
Val Asn Cys Met Glu Leu Lys Arg Ile Cys Lys Cys Leu Thr Ile Ser
1 5 10 15
Ala Val Leu Ser Gln Ile Ala Val Tyr Pro Val Thr Ser Tyr Ala Val
20 25 30
Ser Asn Thr Glu Gln Asn Thr Asp Asn Met Lys Ile Thr Glu Glu Lys
35 40 45
Ser Asn Met Ile Arg Asn Gly Leu Leu Gly Asn Val His Lys Ser Ser
50 55 60
Val Lys Ser Ala Tyr Ser Ser Asn Val Thr Asn Val Asp Glu Gln Met
65 70 75 80
Asn Lys Ile Ser Asp Phe Tyr Tyr Gln Asn Asn Leu Arg Gly Lys Glu
85 90 95
Ile Ser Thr Tyr Tyr Tyr Val Asn Gln Leu Lys Glu Lys Lys Thr Thr
100 105 110
Met Ser Leu Asp Leu Asn Ala Ser Asp Ile Asn Asn Val Thr Tyr Asn
115 120 125
Asp Leu Gln Pro Glu Tyr Ile Gly Glu Asn Glu Phe Gln Asn Thr Thr
130 135 140
Asp Lys Asp Gln Thr Phe Thr Thr Ala Ala Tyr Ser His Ala Val Thr
145 150 155 160
Asp Thr Val Ser Ser Thr Val Thr Asn Gly Phe Lys Ile Gly Gly Ser
165 170 175
Gly Asp Thr Ile Phe Lys Ile Pro Ile Leu Leu Lys Asp Gly Ile Lys
180 185 190
Leu Ser Ala Glu Phe Asn Ser Ala Thr Ser Thr Thr Asn Thr Thr Thr
195 200 205
Asp Thr Lys Thr Leu Thr Ala Ser Pro Gln Asn Ile Lys Val Pro Ala
210 215 220
Gly Lys Thr Tyr Lys Val Val Val Asn Leu Tyr Lys Lys Ser Phe Ala
225 230 235 240
Gly Asp Ile Asp Phe Thr Gly Lys Ala Thr Asn Val Asn Ser Lys Leu
245 250 255
Thr Val Asn Ala Thr Tyr Val Gly Pro Gly Phe Pro Arg Arg Asp Lys
260 265 270
Glu Gln Ser Tyr Thr Tyr Ala Thr Ala Asp Met Leu Lys Asp Leu Thr
275 280 285
Asn Asp Gln Arg Asn Gln Ile Thr Gly Ile Ser Phe Asp Asn Asn Lys
290 295 300
Asn Leu Thr Leu Asn Gly Lys Ala Lys Ile Glu Gly Ile Tyr Gly Ser
305 310 315 320
Lys Leu Gln Val Ser Val Tyr Asp Ile Thr Asn Asn Ala His Arg Leu
325 330 335
Val Gln Val Phe
340
<210> 75
<211> 579
<212> PRT
<213> 芽孢杆菌属的种
<400> 75
Met Glu Tyr Thr Gly Lys Asn Ile Asn Lys Ala Gln Gln Asp Asn Pro
1 5 10 15
Pro Pro Gln Gly Ala Ile Ala Thr Ser Ala Phe Ala Pro Glu Glu Asp
20 25 30
Ser Val Ser Val Ile Ser Ser His Thr Ile Asn Ile Ala Asp Thr Gln
35 40 45
Thr Glu Asn Leu Glu Lys Ser Phe Trp Gln Lys Ala Thr Ser Glu Met
50 55 60
Thr Ile Lys Asn Gly Ala Lys Phe Gly Gly Thr Thr Leu Lys Glu Phe
65 70 75 80
Leu Lys Phe Ala Lys Ser Asn Asp Ser Ala Ser Ala Asn Asn Ala Leu
85 90 95
Arg Ala Ile Val Leu Ala Ser Phe Asp Phe Ile Pro Tyr Gly Met Phe
100 105 110
Ile Ser Pro Ile Ile Gly Tyr Ile Trp Glu Glu Gln Gly Gly Ile Lys
115 120 125
Glu Gln Leu Thr Lys Leu Lys Asp Glu Ile Asn Ala Glu Thr Asp Lys
130 135 140
Lys Ile Ala Gln Gln His Leu Ile Asp Leu Asp Ser Lys Phe Ser Asp
145 150 155 160
Leu Leu Glu Asn Leu Asn Lys Leu Glu Asn Ser Val Lys Ser Lys Lys
165 170 175
Ala Glu Met Tyr Tyr Asp Ser Gln Gly Ser Ile Glu Glu Ser Arg Arg
180 185 190
Ile Trp Val Gly Ile Ile Glu Ser Lys Phe Gly Glu Ile Leu Thr Leu
195 200 205
Ala Lys Gln Glu Lys Asn Lys Val Asn Glu Leu Pro Leu Tyr Thr Gln
210 215 220
Val Ala Thr Ala His Ile Ser Phe Leu Lys Asn Leu Glu Ile Asn Gly
225 230 235 240
Pro Gly Pro Asn Tyr Lys Phe Asp Pro Gln Ser Leu Lys Tyr Phe Tyr
245 250 255
Asn Thr Lys His Ile Gln Glu Thr Ile Asp Lys Tyr Thr Thr Tyr Ile
260 265 270
Lys Asn Thr Phe Asp Asp Phe His Ile Lys Asn Ile Glu Lys Ile Tyr
275 280 285
Lys Val Tyr Gly Ser Pro Ile Asp Pro Tyr Ile Phe Ala Thr Ser Glu
290 295 300
Gly Arg Glu Leu Val Lys Gln Lys Leu Asn Lys Leu Leu Leu Glu Ala
305 310 315 320
Lys Ala Thr Leu Asp Lys Ile Asn Asn Glu Thr Val Trp Leu Gly Gly
325 330 335
Trp Ala Ala His Gly Arg Ser Val Glu Lys Ala Thr Asp Glu Leu Asn
340 345 350
Asn Val Lys Val Ala Val Asn Ala Ala Asp Phe Ser Ser Lys Leu Phe
355 360 365
Glu Thr Thr Ile Ala Asp Glu Ser Phe Asn Thr Ile Ala Ser Leu Gly
370 375 380
Thr Trp Ile Glu Lys Asp Gly Lys His Tyr Tyr Asn Asp Arg Tyr Gly
385 390 395 400
Glu Met Val Thr Glu Trp Leu Asn Ile Gly Lys Lys Thr Tyr Phe Phe
405 410 415
Ser Pro Glu Asp Asn Val Glu Asn Leu Ser Gly Ile Lys Phe Lys Lys
420 425 430
Gly Glu Met Ile Thr Gly Trp Val Asn Thr Glu Glu Asn Met Glu Ser
435 440 445
Asn Gln Ile Arg Ser Tyr Leu Phe Val Asp Glu Glu Glu Gly Leu Lys
450 455 460
Asn Ser Ala Gly Glu Leu Phe Thr Leu Gly Gln Met Met Thr Gly Trp
465 470 475 480
His Lys Thr Met Asp Gly Lys Asp Lys Glu Phe Trp Tyr Tyr Phe Thr
485 490 495
Ser Glu Glu Glu Gly Leu Lys Asn Ser Ala Gly Glu Leu Phe Thr Leu
500 505 510
Gly Gln Met Met Thr Gly Trp His Lys Thr Met Asp Glu Lys Gly Lys
515 520 525
Glu Phe Trp Tyr Tyr Phe Ser Pro Lys Glu Glu Gly Leu Lys Asn Phe
530 535 540
Ala Gly Lys Leu Phe Thr Leu Gly Gln Met Met Thr Gly Thr Val Lys
545 550 555 560
Ile Gly Asn Lys Gly Tyr Thr Phe Ala Glu Asn Gly Val Cys Thr Asn
565 570 575
Pro Asn Ile
<210> 76
<211> 1192
<212> PRT
<213> 芽孢杆菌属的种
<400> 76
Met Asn Lys Asn Tyr Asn Glu Glu His Glu Ile Lys Arg Asn Val Thr
1 5 10 15
Ile Lys Tyr Pro Phe Thr Gln Val Ser Asp Phe Glu Val Asn Thr Thr
20 25 30
Asn Tyr Lys Ser Trp Ile Gly Met Cys Thr Arg Glu Asn Ser Arg Glu
35 40 45
Tyr Gly Lys Tyr Asn Lys Asn Ala Val Ile Ile Ala Thr Gly Ile Gly
50 55 60
Trp Ala Val Leu Gly Phe Ile Pro Val Val Gly Pro Gly Leu Ser Ala
65 70 75 80
Ala Ala Gly Val Leu Asn Val Val Ile Pro Phe Ile Trp Pro Glu Glu
85 90 95
Ala Gly Pro Pro Gly Ser Asn Gln Ala Gln Phe Thr Trp Glu Gln Ile
100 105 110
Met Asn Ala Val Glu Glu Leu Thr Asp Asn Lys Ile Asn Ser Ser Ile
115 120 125
Arg Glu Arg Ala Ile Glu Thr Thr Arg Ile Leu Gln Ser Arg Met Arg
130 135 140
Asp Tyr Gln Gln Ala Ile Cys Asn Leu Lys Glu Asn Thr Asp Lys Asp
145 150 155 160
Lys Asp Lys Glu Tyr Lys Glu Asp Val Arg Arg Glu Phe Asn Asp Ala
165 170 175
Glu Asp Gln Leu Lys Ser Ala Ile Ile Gln Phe Ser His Gln Asp Glu
180 185 190
Asn Pro Asp Ile Glu Ala Glu His Asn Ile Leu Leu Leu Ala Ser Phe
195 200 205
Ala Gln Ala Ala Asn Leu His Leu Leu Leu Leu Lys Asp Val Val Gln
210 215 220
Phe Gly Gln Tyr Trp Gly Phe Thr Glu Glu Gln Val Glu Gln Tyr Tyr
225 230 235 240
Ser Asn Lys Ser Thr Ser Val Gly Asn Pro Gly Met Val Gln Leu Leu
245 250 255
Ala Gln Tyr Thr Asp Tyr Cys Thr Lys Trp Tyr His Asn Gly Leu Asn
260 265 270
Lys Leu Lys Pro Gln Asn Ile Asp Gln Lys Thr Trp Asn Lys Tyr Asn
275 280 285
Asp Phe Arg Arg Asp Met Thr Ile Met Val Leu Asp Ile Val Ser Leu
290 295 300
Trp Pro Thr Tyr Asp Pro Lys Arg Tyr Pro Leu Phe Thr Lys Ser Gln
305 310 315 320
Leu Thr Arg Gln Val Tyr Thr Gln Leu Ile Asp Gly Tyr Tyr Ser Glu
325 330 335
Pro Asn Leu Ala Pro Pro Ser Leu Val Lys Trp Leu Lys Glu Val Thr
340 345 350
Phe Tyr Phe Arg Glu Val Ser Asp Glu Glu Gln Ala Trp Glu Lys Thr
355 360 365
Arg Gly Gln Phe Val Gly Tyr Lys Met Lys Phe Val Thr Thr Met Ala
370 375 380
Asp Asp Cys Tyr Glu Ile Glu Thr Pro Ile Val Gly Glu Met Gly Ala
385 390 395 400
Phe Asp Asp Thr Val Arg Phe Asn Gly Glu Val Tyr Lys Val Ser Ser
405 410 415
Ile Asn Leu Lys Arg Glu Ser Thr Ile Asp Gln Glu Asp Ile Asn Leu
420 425 430
Ala His Tyr Leu Gln Lys Leu Glu Leu Tyr Phe Asp Ser Asn Val Ile
435 440 445
Phe Val Ile Gly Thr Asp Tyr Ala Gly Gln Asn Glu Phe Glu Thr Val
450 455 460
Val Ile Asn Gln Gly Leu Ala Cys Asp Asn Ser Val Gln Glu Ile Cys
465 470 475 480
Glu Pro Cys Met Ser Ser Cys Ala Thr Ser Lys Asp Met Ser Phe Pro
485 490 495
Cys Asp Asn Leu Asp Leu Tyr Ser Glu Ser Leu Ser Trp Val Gly Ala
500 505 510
Leu Tyr Arg Gly Thr Asn Phe Pro Asn Arg Gly Leu Ala Asp Trp Val
515 520 525
Phe Gly Trp Thr His Arg Ser Val Asp Ile Asp Asn Gln Val Leu Pro
530 535 540
Gln Asn Ile Thr Gln Ile Pro Ala Val Lys Ala Lys Glu Ile Ile Ser
545 550 555 560
Gly Gln Val Ile Glu Gly Leu Gly Ser Thr Gly Gly Asn Leu Val Arg
565 570 575
Leu Asp Glu Asn Asn Gln Met Leu Leu Gly Val Thr Ile Val Ser Asp
580 585 590
Leu Ser Glu Lys Ile Ser Gly Tyr Tyr Ile Arg Leu Arg Tyr Ala Ser
595 600 605
Asn Gln Asn Asn Arg Leu Thr Val Ser Tyr Tyr Phe Glu Lys Glu Gln
610 615 620
Val Asp Ser Ile Glu Phe Asp Val Leu Lys Thr Tyr Asp Gly Gln Asp
625 630 635 640
Leu Trp Tyr Cys Ala Phe Gly His Gln Thr Val Met Lys Ile Pro Asn
645 650 655
Pro Lys Glu Thr Lys Glu Tyr Thr Ile Lys Ile Glu Asn Lys Gly Asn
660 665 670
Gly Ser Val Ile Ile Asp Lys Val Glu Phe Leu Pro Ile Glu Asp Thr
675 680 685
Leu Glu Ala Tyr Gln Glu Asn Glu Asn Leu Glu Arg Ala Arg Asn Ala
690 695 700
Val Asn Ala Leu Phe Ile Asn Glu Ala Lys Asp Thr Leu Arg Leu Asn
705 710 715 720
Thr Thr Asp Tyr Glu Ile Asp Gln Val Ser Lys Leu Val Glu Cys Leu
725 730 735
Ser Asp Glu Leu Ser Thr Tyr Glu Thr Ile Val Leu Leu Asp Gln Val
740 745 750
Lys Phe Ala Lys Gln Leu Ser Lys Lys Arg Asn Leu Leu Asn Tyr Gly
755 760 765
Asp Phe Glu Phe Leu Asn Trp Ser Gly Arg Asn Gly Trp Lys Ala Ser
770 775 780
Pro His Val Tyr Val Lys Ala Asp Asn Gln Ile Phe Lys Gly His Tyr
785 790 795 800
Leu His Met Pro Ala Ala Ile His Ser Lys Leu Asn Asp Thr Ile Tyr
805 810 815
Pro Thr Tyr Val Tyr Gln Lys Val Asp Glu Ser Lys Leu Lys Ser Tyr
820 825 830
Thr Arg Tyr Leu Val Arg Gly Phe Ile Ser Asp Ser Lys Asp Leu Glu
835 840 845
Leu Leu Val Glu Arg Tyr Gly Glu Asp Val His Val Lys Met Asp Val
850 855 860
Pro Asn Asn Ile Phe Tyr Asn Leu Ser Ile Gly Glu Ser Thr Lys His
865 870 875 880
Ile Lys Thr Ser Gly Ser Tyr Lys Gly Leu Asn Ala Phe Ser Phe Tyr
885 890 895
Ile Asp Thr Gly Leu Val Glu Ile Lys Arg Asn Leu Gly Ile Ile Phe
900 905 910
Ala Leu Lys Ile Thr Ser Ile Asn Gly Ser Ala Asn Val Gly Asn Leu
915 920 925
Glu Ile Ile Glu Glu Gln Pro Leu Val Gly Glu Ala Leu Lys Arg Val
930 935 940
Lys Lys Arg Glu Gln Glu Trp Glu Glu Lys Met Lys His Gln Cys Tyr
945 950 955 960
Glu Thr Asp Met Val Val Arg Asp Thr Arg Asn Ala Ile Asn Thr Leu
965 970 975
Phe Thr Ser Leu Gln Tyr Asn Glu Leu Lys Phe Asp Thr Leu Phe Phe
980 985 990
His Ile Leu Lys Ala Asn Asn Leu Val Gln Gln Ile Pro Tyr Val Cys
995 1000 1005
His Pro Phe Leu Met Lys Gly Asn Pro Val Val Pro Gly Met Asn
1010 1015 1020
Phe Asn Ile Phe Gln Gln Leu Leu Thr Leu Val Glu Lys Ala Lys
1025 1030 1035
Glu Leu Tyr Tyr Leu Arg Asn Val Val Pro Asn Gly Thr Phe Asn
1040 1045 1050
Glu Gly Ile Gly Asn Trp Cys Val Ser Asn Gly Val Arg Ile Lys
1055 1060 1065
Gln Glu Gly Asn Asn Ala Val Leu Ile Leu Arg Glu Trp Gln Asp
1070 1075 1080
Lys Ala Ile Gln Arg Ser Pro Ile Asp Ala Glu Gln Gly Tyr Phe
1085 1090 1095
Leu Arg Val Thr Ala Arg Lys Glu Gly Ser Gly Phe Gly Tyr Val
1100 1105 1110
Val Ile His Asp Ser Asp Asn Met Ile Glu Lys Leu Lys Phe Thr
1115 1120 1125
Ser Cys Asp His Asp Thr Asp Asp Thr Val Ser Val Asp Ser Leu
1130 1135 1140
Met Gly Tyr Val Thr Lys Thr Ala Glu Ile Phe Pro Gly Thr Asn
1145 1150 1155
Gln Val Cys Ile Glu Ile Gly Glu Thr Ala Gly Val Phe Lys Ile
1160 1165 1170
Val Ser Val Glu Leu Ile Lys Thr Arg Asp Met Lys Lys Tyr Ile
1175 1180 1185
Leu Asp Gly Met
1190
<210> 77
<211> 685
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 77
Met Asn Lys Asn Tyr Asn Glu Glu His Glu Ile Lys Arg Asn Val Thr
1 5 10 15
Ile Lys Tyr Pro Phe Thr Gln Val Ser Asp Phe Glu Val Asn Thr Thr
20 25 30
Asn Tyr Lys Ser Trp Ile Gly Met Cys Thr Arg Glu Asn Ser Arg Glu
35 40 45
Tyr Gly Lys Tyr Asn Lys Asn Ala Val Ile Ile Ala Thr Gly Ile Gly
50 55 60
Trp Ala Val Leu Gly Phe Ile Pro Val Val Gly Pro Gly Leu Ser Ala
65 70 75 80
Ala Ala Gly Val Leu Asn Val Val Ile Pro Phe Ile Trp Pro Glu Glu
85 90 95
Ala Gly Pro Pro Gly Ser Asn Gln Ala Gln Phe Thr Trp Glu Gln Ile
100 105 110
Met Asn Ala Val Glu Glu Leu Thr Asp Asn Lys Ile Asn Ser Ser Ile
115 120 125
Arg Glu Arg Ala Ile Glu Thr Thr Arg Ile Leu Gln Ser Arg Met Arg
130 135 140
Asp Tyr Gln Gln Ala Ile Cys Asn Leu Lys Glu Asn Thr Asp Lys Asp
145 150 155 160
Lys Asp Lys Glu Tyr Lys Glu Asp Val Arg Arg Glu Phe Asn Asp Ala
165 170 175
Glu Asp Gln Leu Lys Ser Ala Ile Ile Gln Phe Ser His Gln Asp Glu
180 185 190
Asn Pro Asp Ile Glu Ala Glu His Asn Ile Leu Leu Leu Ala Ser Phe
195 200 205
Ala Gln Ala Ala Asn Leu His Leu Leu Leu Leu Lys Asp Val Val Gln
210 215 220
Phe Gly Gln Tyr Trp Gly Phe Thr Glu Glu Gln Val Glu Gln Tyr Tyr
225 230 235 240
Ser Asn Lys Ser Thr Ser Val Gly Asn Pro Gly Met Val Gln Leu Leu
245 250 255
Ala Gln Tyr Thr Asp Tyr Cys Thr Lys Trp Tyr His Asn Gly Leu Asn
260 265 270
Lys Leu Lys Pro Gln Asn Ile Asp Gln Lys Thr Trp Asn Lys Tyr Asn
275 280 285
Asp Phe Arg Arg Asp Met Thr Ile Met Val Leu Asp Ile Val Ser Leu
290 295 300
Trp Pro Thr Tyr Asp Pro Lys Arg Tyr Pro Leu Phe Thr Lys Ser Gln
305 310 315 320
Leu Thr Arg Gln Val Tyr Thr Gln Leu Ile Asp Gly Tyr Tyr Ser Glu
325 330 335
Pro Asn Leu Ala Pro Pro Ser Leu Val Lys Trp Leu Lys Glu Val Thr
340 345 350
Phe Tyr Phe Arg Glu Val Ser Asp Glu Glu Gln Ala Trp Glu Lys Thr
355 360 365
Arg Gly Gln Phe Val Gly Tyr Lys Met Lys Phe Val Thr Thr Met Ala
370 375 380
Asp Asp Cys Tyr Glu Ile Glu Thr Pro Ile Val Gly Glu Met Gly Ala
385 390 395 400
Phe Asp Asp Thr Val Arg Phe Asn Gly Glu Val Tyr Lys Val Ser Ser
405 410 415
Ile Asn Leu Lys Arg Glu Ser Thr Ile Asp Gln Glu Asp Ile Asn Leu
420 425 430
Ala His Tyr Leu Gln Lys Leu Glu Leu Tyr Phe Asp Ser Asn Val Ile
435 440 445
Phe Val Ile Gly Thr Asp Tyr Ala Gly Gln Asn Glu Phe Glu Thr Val
450 455 460
Val Ile Asn Gln Gly Leu Ala Cys Asp Asn Ser Val Gln Glu Ile Cys
465 470 475 480
Glu Pro Cys Met Ser Ser Cys Ala Thr Ser Lys Asp Met Ser Phe Pro
485 490 495
Cys Asp Asn Leu Asp Leu Tyr Ser Glu Ser Leu Ser Trp Val Gly Ala
500 505 510
Leu Tyr Arg Gly Thr Asn Phe Pro Asn Arg Gly Leu Ala Asp Trp Val
515 520 525
Phe Gly Trp Thr His Arg Ser Val Asp Ile Asp Asn Gln Val Leu Pro
530 535 540
Gln Asn Ile Thr Gln Ile Pro Ala Val Lys Ala Lys Glu Ile Ile Ser
545 550 555 560
Gly Gln Val Ile Glu Gly Leu Gly Ser Thr Gly Gly Asn Leu Val Arg
565 570 575
Leu Asp Glu Asn Asn Gln Met Leu Leu Gly Val Thr Ile Val Ser Asp
580 585 590
Leu Ser Glu Lys Ile Ser Gly Tyr Tyr Ile Arg Leu Arg Tyr Ala Ser
595 600 605
Asn Gln Asn Asn Arg Leu Thr Val Ser Tyr Tyr Phe Glu Lys Glu Gln
610 615 620
Val Asp Ser Ile Glu Phe Asp Val Leu Lys Thr Tyr Asp Gly Gln Asp
625 630 635 640
Leu Trp Tyr Cys Ala Phe Gly His Gln Thr Val Met Lys Ile Pro Asn
645 650 655
Pro Lys Glu Thr Lys Glu Tyr Thr Ile Lys Ile Glu Asn Lys Gly Asn
660 665 670
Gly Ser Val Ile Ile Asp Lys Val Glu Phe Leu Pro Ile
675 680 685
<210> 78
<211> 205
<212> PRT
<213> 芽孢杆菌属的种
<400> 78
Met Asn Arg Gly Thr Ile Ser Asp Lys Lys Lys Ser Gly Tyr Ser Glu
1 5 10 15
Glu Glu Tyr Tyr Leu Lys Leu Ile Ser Asp Val Lys Pro Glu Tyr Thr
20 25 30
Val Gln Thr Leu Asn Leu Met Asp Lys Phe Lys Phe Ile Val Asn Pro
35 40 45
Met Thr Lys Glu Leu Asp Thr Ser Lys Ile Leu Gln Ile Ala Asn Glu
50 55 60
His Pro Asp Leu Ala Ile Val Ser Thr Leu Asn Gln Gln Phe Thr Ser
65 70 75 80
Asn Gln Lys Met Pro Ile Lys Asn Leu Val Asp Ser Ser Lys Lys Phe
85 90 95
Val Phe Ser Thr Ile Gly Asp Ser Tyr Leu Asp Lys Gly Ile Lys Lys
100 105 110
Thr Cys Ile Asn Trp Ile Glu Asn Val Ile Val Lys Leu Asn Ser Gln
115 120 125
Ile Glu His Pro Trp Ile Ser Phe Tyr Thr Glu Ala Lys Asn Lys Thr
130 135 140
Ser Tyr Arg Tyr Asn Ile Leu Phe Ile Phe Gln Arg Ala Ser Phe Leu
145 150 155 160
Val Met Thr Pro Ile Ser Phe Glu Ile Thr Ile Leu Ala Ser Lys Asp
165 170 175
Thr Val Leu Asp Leu Lys Leu Thr Asp Lys Ala Glu Tyr His Val Ser
180 185 190
Ile Lys Leu Ile Gln Val Ile Gln Ser Leu Asn Pro Cys
195 200 205
<210> 79
<211> 463
<212> PRT
<213> 芽孢杆菌属的种
<400> 79
Val Ile Asp Asn Asn Asn Gln Thr Thr Asn Ile Met Asp Gln Phe Ser
1 5 10 15
Asp Leu Met Asn Glu Lys Ile Lys Asn Tyr Asn Arg Gln Asn Ile Lys
20 25 30
Ala Thr Leu Lys Ala Leu His His Glu Leu Lys Ala Leu Asp Asp Ser
35 40 45
Leu Asn Gly Lys Arg Val Ser Glu Ser Tyr Tyr Ala Ser Asp Ser Pro
50 55 60
Asp Glu His Asn Arg Asn Arg Val Arg Phe Ile His Ser Asn Phe Lys
65 70 75 80
Ala Leu Ile Leu Gln Cys Ser Lys Glu Gly Tyr Glu Glu Pro Glu Leu
85 90 95
Pro Leu Tyr Thr Ala Val Ala Thr Ala His Ile Leu Phe Leu Arg Cys
100 105 110
Ile Glu Lys His Gly Leu Gly Pro Lys Leu Gln Phe His Ile Ser Asp
115 120 125
Tyr Asn Glu Phe Met Lys Asp Leu Lys Asp Tyr Pro Asp Lys Tyr Ile
130 135 140
Lys His Ile Lys Glu Thr Tyr Pro Lys Gly Ser Ser Lys Leu Phe Lys
145 150 155 160
Glu Pro Thr Leu Val Pro Lys Tyr Tyr Ala Glu Thr Lys Gly Asn Lys
165 170 175
Ala Phe Trp Met Ala Val Lys Arg Asn Thr Leu Val Pro Gln Asn Gly
180 185 190
Lys Trp Tyr Tyr Leu Asp Arg Asp Gly Lys Met Lys Thr Gly Trp Val
195 200 205
Lys Thr Gly Gly Phe Ile Met Asp Asp Asn Gly Gln Leu Glu Met His
210 215 220
Gln Pro Asp Ala Tyr Tyr Tyr Phe Ser Ser Asn Gly Pro Met Val Thr
225 230 235 240
Gly Trp Phe Thr Asp Ala Asp Gly Arg Thr Tyr Tyr Leu Asn Pro Glu
245 250 255
Glu Thr Gln Lys Phe Tyr Lys Gly Gln Met Ala Glu Gly Phe Val Glu
260 265 270
Ile Gly Lys Gly Lys Trp Phe Tyr Phe Asn Pro Leu Asp Ile Ser Arg
275 280 285
Leu Ala Pro Asn Gly Tyr Asp Asn Leu Pro Glu Ala Gly Lys Lys Leu
290 295 300
Leu Lys Asp Thr Phe Gly Ile Ser Lys Asn Ile Leu Arg Gly Glu Gln
305 310 315 320
Arg Thr Glu Trp Ile His Asp Asn Gly Ala Trp Tyr Tyr Cys Ser Pro
325 330 335
Glu Lys Asn Asn Arg Phe Asp Lys Gly Gln Met Val Thr Gly Glu Val
340 345 350
Asn Ile Lys Asn Ser Glu Gly Asn Ile Lys Thr Tyr Gln Phe Asn Asn
355 360 365
Asp Gly Ala Cys Thr Asn Thr Tyr Gln Lys Met Val Leu Gln Tyr Phe
370 375 380
Met Val Val Val Pro Phe Phe Ile Ala Tyr Gln Leu Phe Tyr Thr Asn
385 390 395 400
Glu Tyr Leu Asn Leu Glu Ile Ile Phe Cys Pro Ser Lys Leu Phe Lys
405 410 415
Thr Phe Phe Asn Leu Gly Glu Leu Thr Tyr Tyr Phe Lys Arg Arg Ser
420 425 430
Ser Leu Tyr Leu Ser Asn Phe Cys Tyr Pro Thr Ile Thr Arg Ile Leu
435 440 445
Ile Leu Leu Tyr Ile Ile Thr Leu Leu Lys Phe Tyr Asn Tyr Leu
450 455 460
<210> 80
<211> 323
<212> PRT
<213> 芽孢杆菌属的种
<400> 80
Met Arg Asn Asn Lys Lys Trp Lys Arg Lys Val Phe Ser Leu Ala Thr
1 5 10 15
Ile Ala Thr Ile Gly Val Ser Cys Thr Thr Leu Pro Asn His Ala Ser
20 25 30
Ala Asp Thr Thr Glu Lys Val Pro Gln Asp Val Ser Lys Ile Gln Glu
35 40 45
Gln Asn Lys Lys Gln Gln Gly Ile Gln Asp Trp Gln Gly Pro Ile Asp
50 55 60
Phe Thr Ile Gly Gln Phe Phe Gln Lys Lys Thr Gly Asn Asn Val Ala
65 70 75 80
His Ile Leu Thr Arg Tyr Asp Asn Pro Lys Phe Ile Glu Thr Thr Ala
85 90 95
Val Ala Ser Ser Ser Pro Lys Ile Thr Asp Leu Lys Glu Val Tyr Val
100 105 110
Gly Arg Ser Ile Leu Arg Asn Asn Thr Asp Ile Asp Gln Thr Leu Tyr
115 120 125
Thr Asp Ser Tyr Ser Glu Thr Ile Thr Glu Ser Thr Thr Ser Ala Val
130 135 140
Thr His Gly Phe Asn Ile Gly Glu Ser Thr Thr Ala Ser Leu Ser Leu
145 150 155 160
Pro Ile Val Gly Ala Gly Phe Glu Gln Glu Val His Met Glu Tyr Asn
165 170 175
Ile Ser Thr Glu Asn Ser Thr Thr Lys Glu Lys Ser Arg Thr Tyr Thr
180 185 190
Ala Pro Ser Gln Ala Ile Val Val Pro Ala His Ser Ser Val Glu Val
195 200 205
Ile Val Lys Leu Asp Arg Ile Lys Ala Asp Gly Lys Val Asp Leu Phe
210 215 220
Ser Lys Met Lys Asp Thr Gln Tyr Thr Ser Leu Val Trp Phe Lys Asp
225 230 235 240
Arg Pro Asp Gln Met Phe Val Gly Ser Val Tyr Asp Ala Val Arg Asp
245 250 255
Val Thr Ser Asp Gln Thr Phe Pro Asp Ile Lys Ala Asn Lys Lys Asp
260 265 270
Lys Thr Ile Ser Leu Ile Gly Lys Gly Asn Tyr Lys Ala Val Leu Gly
275 280 285
Thr Thr Phe Ser Val Asn Val Lys Pro Val Asn Lys Glu Gly Arg Ala
290 295 300
Ile Asn Asp Gly Tyr Thr Tyr Lys Val Asn Pro Gln Ile Thr Asp Val
305 310 315 320
Lys Ile Lys
<210> 81
<211> 270
<212> PRT
<213> 芽孢杆菌属的种
<400> 81
Met Asn Ile Asp Ala Leu Asn Lys Asn Glu Ile Lys Leu Ser Asn Ala
1 5 10 15
Asn Gln Asn Val Cys Asp Asn Phe Leu His Ile Thr Leu Asn Leu Pro
20 25 30
Val Glu Glu Glu Lys Asn Phe Thr Glu Ile Tyr Gln Val Glu Pro Gln
35 40 45
Tyr Leu Ala Gln Ala Leu Arg Leu Ala Asn Ala Phe Gln Gly Ala Ile
50 55 60
Asp Pro Ser Asp Leu Asn Phe Asn Phe Glu Arg Ala Leu Asn Ile Ala
65 70 75 80
Asn Asn Ile Pro Asn Ser Ala Ile Val Lys Thr Met Asn Gln Asn Val
85 90 95
Val Gln Gln Thr Val Gln Ile Ser Val Met Ile Asp Gln Leu Lys Glu
100 105 110
Leu Ile Arg Thr Val Leu Gly Phe Ala Ile Ser Ser Pro Thr Phe Trp
115 120 125
Ser Ser Val Glu Ala Ala Ile Thr Ser Thr Phe Thr Asn Leu Asn Thr
130 135 140
Gln Glu Asp Ser Ala Trp Ile Phe Trp Lys Ser Glu Ser Ser Ser Asn
145 150 155 160
Thr Thr Tyr Tyr Tyr Asn Ile Leu Phe Ser Ile Gln Asn Ala Glu Thr
165 170 175
Gly Gly Val Met Ala Val Leu Pro Ile Ala Phe Glu Ile Ser Val Asn
180 185 190
Leu Glu Lys Lys Lys Val Leu Phe Leu Thr Ile Arg Asp Ser Ala Arg
195 200 205
Tyr Glu Val Arg Val Lys Ala Ile Thr Leu Ala Gln Ala Leu Asp Arg
210 215 220
Asn Gln Arg Asn Ser Thr Asp Ile Phe Asn Met His Lys Asn Asn Pro
225 230 235 240
Tyr Gln Ala Ile Glu Asn Lys Val Phe Asn Glu Glu Met Ile Asn Gln
245 250 255
Leu Met His Lys Pro Val Asn Thr Ser Gly Ile Ile Leu Pro
260 265 270
<210> 82
<211> 1281
<212> PRT
<213> 芽孢杆菌属的种
<400> 82
Met His Gln Asn Tyr Ser Glu Glu Tyr Glu Ile Met Asp Thr Val Gly
1 5 10 15
Met Asn His Gln Ser Arg Tyr Pro Leu Ala Lys Ala Pro Gly Ser Glu
20 25 30
Phe Gln Gln Met Asn Tyr Lys Asn Trp Leu Asn Met Cys Val Ser Gly
35 40 45
Glu Ser Gly Glu Leu Phe Ala Asn Ser Asn Thr Ala Arg Asn Gly Val
50 55 60
Ile Ile Gly Thr Gly Ile Ala Trp Ala Val Leu Thr Leu Ile Pro Gly
65 70 75 80
Tyr Gly Thr Gly Leu Ser Ala Leu Ala Gly Val Leu Asn Val Ile Ile
85 90 95
Pro Tyr Leu Trp Pro Glu Glu Ala Ala Pro Glu Val Pro Ser Gln Phe
100 105 110
Ser Trp Glu Gln Leu Met Thr Ala Ala Glu Glu Leu Ile Asp Lys Lys
115 120 125
Val Asp Ala Tyr Asp Arg Asn Arg Ala Leu Thr Glu Leu Ala Gly Ile
130 135 140
Gln Gln Ala Ile Lys Leu Tyr Gln Asp Ala Ala Cys Asp Leu Lys Lys
145 150 155 160
Asp Pro Thr Asn Thr Gln Leu Gln Asp Thr Val Arg Ala Gln Phe Ile
165 170 175
Ser Thr Asn Thr Thr Ile Tyr Asn Arg Met Pro Thr Phe Arg Ala Ser
180 185 190
Gly Phe Glu Val Ser Leu Leu Ser Ile Tyr Ser Gln Ala Ala Asn Leu
195 200 205
His Leu Ala Phe Leu Lys Asp Gly Val Gln Phe Gly Ala Glu Trp Gly
210 215 220
Met Asp Pro Thr Thr Val Asp Arg Tyr Tyr Gly Tyr Leu Thr Ala Asn
225 230 235 240
Thr Ala Thr Tyr Thr Asp Tyr Cys Val Tyr Trp Tyr Asn Phe Gly Leu
245 250 255
Asn Ala Leu Ile Gln Lys Ala Asn Glu Val Pro Val Tyr Ser Thr Ala
260 265 270
Tyr Trp Asn Lys Pro Asn Asn Phe Arg Arg Asp Met Thr Ile Met Val
275 280 285
Leu Asp Ile Val Ser Leu Trp Pro Thr Tyr Asn Pro Arg Ile Tyr Pro
290 295 300
Gln Thr Thr Lys Ala Gln Leu Thr Arg Ser Val Tyr Thr Pro Ile Ser
305 310 315 320
Gly Asn Ile Pro Arg Ser Phe Glu Gln Val Asp Asp Ala Val Ile Pro
325 330 335
Pro Ala Ser Leu Phe Ser Trp Leu Arg Glu Val Thr Leu Phe Arg Arg
340 345 350
Ala Phe Ala Thr Pro Thr Asn Glu Tyr Thr Leu Phe Thr Gln Tyr Ala
355 360 365
Gly Tyr Lys Met Ala Phe Gln Asn Thr Leu Ala Ser Thr Leu Gln Glu
370 375 380
Thr Pro Val Phe Gly Glu Arg Gly Asp Glu Val Thr Ser Val Gly Ile
385 390 395 400
Gly Glu Glu Pro Asn Phe Glu Val Tyr Lys Met Arg Asn Ile Ile Leu
405 410 415
Gly Pro Glu Ala Ser Arg Ser Phe Glu Ser Pro Gln Thr Phe Asp Phe
420 425 430
Tyr Phe Thr Pro Ser Gly Thr Val Glu Thr Val Gly Ile Asn Tyr Ala
435 440 445
Glu Ile Gln Glu Glu Pro Thr Asn Thr Thr Asn Gln Gly Leu Ala Cys
450 455 460
Ile Ser Thr Lys Asp Pro Asp Thr Cys Asp Pro Cys Thr Thr Thr Cys
465 470 475 480
Ser Val Gly Pro Ile Asn Pro Thr Ile Pro Cys Asp Ser Leu Thr Leu
485 490 495
Tyr Ser His Arg Leu Ser Arg Val Gly Gly Val Leu Gly Ala Asn Pro
500 505 510
Gln Ser Ile Ile Gly Leu Thr Tyr Gly Trp Thr His Ile Ser Ala Asp
515 520 525
Asn Asn Asn Thr Ile Asp Thr Glu Lys Ile Thr Gln Ile Pro Ala Val
530 535 540
Lys Gly Phe Leu Leu Tyr Gly Asp Ala Ser Val Val Lys Gly Pro Gly
545 550 555 560
Ser Thr Gly Gly Asp Leu Val Arg Leu Ser Pro Ser Glu Gln Arg Ser
565 570 575
Gly Leu Phe Arg Ile Gly Phe Thr His Pro Leu Ile Asp Thr Leu Pro
580 585 590
Lys Gly Tyr Thr Ile Arg Leu Arg Tyr Thr Ser Asn Ala Asp Val Thr
595 600 605
Leu Phe Ser Val Leu Glu Ile His Ser Asp Glu Gly Met Lys Asp Thr
610 615 620
Asn Asn Thr Phe Thr Leu Pro Ala Thr Ser Ser Asn Asn Ser Leu Asn
625 630 635 640
Tyr Asn Ser Phe Gly Tyr Tyr Thr Leu Ser Gly Leu Glu Leu Glu Ile
645 650 655
Leu Pro Gly Tyr Glu Gln Thr Pro Val Leu His Leu Asp His Trp Ser
660 665 670
Gly Gly Asp Ile Ile Leu Asp Lys Ile Glu Phe Ile Pro Ile Glu Gly
675 680 685
Ser Val Glu Glu Tyr Gln Ala Asn Gln Asp Leu Glu Lys Ala Arg Lys
690 695 700
Ala Val Asn Ala Leu Tyr Thr Ser Asp Ala Lys Ser Ala Leu Lys Leu
705 710 715 720
Asn Ile Thr Asp Tyr Val Val Asp Gln Ala Ala Asn Leu Val Glu Cys
725 730 735
Val Ser Asp Asp Phe His Val Gln Glu Lys Met Ile Leu Leu Asp Gln
740 745 750
Val Lys Val Ala Lys Arg Leu Ser Arg Ala Arg Asn Leu Leu Asn Tyr
755 760 765
Gly Asp Phe Glu Ser Thr Asp Trp Ser Gly Glu Asn Gly Trp Arg Thr
770 775 780
Ser Gln His Val His Val Ala Ala Asn Asn Pro Ile Phe Lys Gly Arg
785 790 795 800
Tyr Leu His Met Pro Gly Ala Thr Ser Ser Gln Phe Ser Ser Asn Val
805 810 815
Tyr Pro Thr Tyr Ile Tyr Gln Lys Val Asp Glu Ser Lys Leu Lys Ser
820 825 830
Tyr Thr Arg Tyr Leu Val Arg Gly Phe Val Gly Asn Ser Lys Asp Leu
835 840 845
Glu Leu Leu Val Glu Arg Tyr Gly Lys Asp Ile His Val Glu Met Asp
850 855 860
Val Pro Asn Asp Ile Gln Tyr Ser Leu Leu Met Asn Glu Cys Gly Gly
865 870 875 880
Phe Asp Arg Cys Arg Pro Ala Leu Ser Gln Thr Arg Ser Asp His Thr
885 890 895
Cys Thr Cys Lys Asp Thr Thr Ser Met His Thr Asp Cys Gln Cys Lys
900 905 910
Asp Lys Ser Asn Arg Thr Ala Thr Gly Val Tyr Thr Asn Val Ser Pro
915 920 925
Gly Ser Thr Met Tyr Thr Asp Arg Phe His Ala His Lys Ser Cys Gly
930 935 940
Cys Lys Asn Asn Asp Met Tyr Gln Lys Gly Thr His Pro Tyr Thr Ser
945 950 955 960
Cys Gly Cys Thr Asp Ser His Val Phe Ser Tyr His Ile Asp Thr Gly
965 970 975
Cys Val Asp Gln Glu Glu Asn Leu Gly Leu Trp Phe Ala Leu Lys Ile
980 985 990
Ala Ser Glu Arg Gly Val Ala Asn Ile Asp Asn Leu Glu Ile Ile Glu
995 1000 1005
Ala Gln Pro Leu Thr Gly Glu Ala Leu Ala Arg Val Lys Lys Arg
1010 1015 1020
Glu Gln Lys Trp Lys Gln Glu Met Val Lys Lys Arg Leu Glu Thr
1025 1030 1035
Glu Lys Ala Val Gln Ala Ala Gln Gly Ala Ile Gln Pro Leu Phe
1040 1045 1050
Thr Asn Ala Gln Tyr Asn Arg Leu Gln Phe Glu Thr Leu Phe Pro
1055 1060 1065
Gln Ile Val His Ala Asp Trp Leu Val Gln Gln Ile Pro Tyr Val
1070 1075 1080
His His Pro Phe Leu Ser Gly Ala Leu Pro Ala Val Pro Gly Met
1085 1090 1095
Asn Phe Glu Ile Val Gln His Leu Leu Thr Val Ile Gly Asn Ala
1100 1105 1110
Arg Ala Leu Tyr Glu Arg Arg Asn Leu Val Arg Asn Gly Thr Phe
1115 1120 1125
Ser Ser Gly Thr Gly Asn Trp His Val Ser Glu Gly Val Lys Val
1130 1135 1140
Gln Pro Leu Gln Asn Thr Ser Val Leu Val Leu Ser Glu Trp Asn
1145 1150 1155
His Glu Ala Ala Gln Gln Leu Arg Ile Asp Pro Asp Arg Gly Tyr
1160 1165 1170
Val Leu Arg Ile Thr Ala Arg Lys Glu Gly Ala Gly Lys Gly Thr
1175 1180 1185
Val Thr Met Ser Asp Cys Ala Ala Tyr Thr Glu Thr Leu Thr Phe
1190 1195 1200
Thr Ser Cys Asp Tyr Asn Thr Val Gly Ser Gln Thr Met Thr Gly
1205 1210 1215
Gly Thr Leu Ser Gly Phe Val Thr Lys Thr Leu Glu Ile Phe Pro
1220 1225 1230
Asp Thr Asp Arg Ile Arg Ile Asp Ile Gly Glu Thr Glu Gly Thr
1235 1240 1245
Phe Lys Ile Glu Ser Val Glu Leu Ile Cys Met Glu Gln Met Glu
1250 1255 1260
Asn His Leu Tyr Asp Met Ala Gly Asn Leu Lys Glu Glu Val Leu
1265 1270 1275
Leu Gln Ser
1280
<210> 83
<211> 686
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 83
Met His Gln Asn Tyr Ser Glu Glu Tyr Glu Ile Met Asp Thr Val Gly
1 5 10 15
Met Asn His Gln Ser Arg Tyr Pro Leu Ala Lys Ala Pro Gly Ser Glu
20 25 30
Phe Gln Gln Met Asn Tyr Lys Asn Trp Leu Asn Met Cys Val Ser Gly
35 40 45
Glu Ser Gly Glu Leu Phe Ala Asn Ser Asn Thr Ala Arg Asn Gly Val
50 55 60
Ile Ile Gly Thr Gly Ile Ala Trp Ala Val Leu Thr Leu Ile Pro Gly
65 70 75 80
Tyr Gly Thr Gly Leu Ser Ala Leu Ala Gly Val Leu Asn Val Ile Ile
85 90 95
Pro Tyr Leu Trp Pro Glu Glu Ala Ala Pro Glu Val Pro Ser Gln Phe
100 105 110
Ser Trp Glu Gln Leu Met Thr Ala Ala Glu Glu Leu Ile Asp Lys Lys
115 120 125
Val Asp Ala Tyr Asp Arg Asn Arg Ala Leu Thr Glu Leu Ala Gly Ile
130 135 140
Gln Gln Ala Ile Lys Leu Tyr Gln Asp Ala Ala Cys Asp Leu Lys Lys
145 150 155 160
Asp Pro Thr Asn Thr Gln Leu Gln Asp Thr Val Arg Ala Gln Phe Ile
165 170 175
Ser Thr Asn Thr Thr Ile Tyr Asn Arg Met Pro Thr Phe Arg Ala Ser
180 185 190
Gly Phe Glu Val Ser Leu Leu Ser Ile Tyr Ser Gln Ala Ala Asn Leu
195 200 205
His Leu Ala Phe Leu Lys Asp Gly Val Gln Phe Gly Ala Glu Trp Gly
210 215 220
Met Asp Pro Thr Thr Val Asp Arg Tyr Tyr Gly Tyr Leu Thr Ala Asn
225 230 235 240
Thr Ala Thr Tyr Thr Asp Tyr Cys Val Tyr Trp Tyr Asn Phe Gly Leu
245 250 255
Asn Ala Leu Ile Gln Lys Ala Asn Glu Val Pro Val Tyr Ser Thr Ala
260 265 270
Tyr Trp Asn Lys Pro Asn Asn Phe Arg Arg Asp Met Thr Ile Met Val
275 280 285
Leu Asp Ile Val Ser Leu Trp Pro Thr Tyr Asn Pro Arg Ile Tyr Pro
290 295 300
Gln Thr Thr Lys Ala Gln Leu Thr Arg Ser Val Tyr Thr Pro Ile Ser
305 310 315 320
Gly Asn Ile Pro Arg Ser Phe Glu Gln Val Asp Asp Ala Val Ile Pro
325 330 335
Pro Ala Ser Leu Phe Ser Trp Leu Arg Glu Val Thr Leu Phe Arg Arg
340 345 350
Ala Phe Ala Thr Pro Thr Asn Glu Tyr Thr Leu Phe Thr Gln Tyr Ala
355 360 365
Gly Tyr Lys Met Ala Phe Gln Asn Thr Leu Ala Ser Thr Leu Gln Glu
370 375 380
Thr Pro Val Phe Gly Glu Arg Gly Asp Glu Val Thr Ser Val Gly Ile
385 390 395 400
Gly Glu Glu Pro Asn Phe Glu Val Tyr Lys Met Arg Asn Ile Ile Leu
405 410 415
Gly Pro Glu Ala Ser Arg Ser Phe Glu Ser Pro Gln Thr Phe Asp Phe
420 425 430
Tyr Phe Thr Pro Ser Gly Thr Val Glu Thr Val Gly Ile Asn Tyr Ala
435 440 445
Glu Ile Gln Glu Glu Pro Thr Asn Thr Thr Asn Gln Gly Leu Ala Cys
450 455 460
Ile Ser Thr Lys Asp Pro Asp Thr Cys Asp Pro Cys Thr Thr Thr Cys
465 470 475 480
Ser Val Gly Pro Ile Asn Pro Thr Ile Pro Cys Asp Ser Leu Thr Leu
485 490 495
Tyr Ser His Arg Leu Ser Arg Val Gly Gly Val Leu Gly Ala Asn Pro
500 505 510
Gln Ser Ile Ile Gly Leu Thr Tyr Gly Trp Thr His Ile Ser Ala Asp
515 520 525
Asn Asn Asn Thr Ile Asp Thr Glu Lys Ile Thr Gln Ile Pro Ala Val
530 535 540
Lys Gly Phe Leu Leu Tyr Gly Asp Ala Ser Val Val Lys Gly Pro Gly
545 550 555 560
Ser Thr Gly Gly Asp Leu Val Arg Leu Ser Pro Ser Glu Gln Arg Ser
565 570 575
Gly Leu Phe Arg Ile Gly Phe Thr His Pro Leu Ile Asp Thr Leu Pro
580 585 590
Lys Gly Tyr Thr Ile Arg Leu Arg Tyr Thr Ser Asn Ala Asp Val Thr
595 600 605
Leu Phe Ser Val Leu Glu Ile His Ser Asp Glu Gly Met Lys Asp Thr
610 615 620
Asn Asn Thr Phe Thr Leu Pro Ala Thr Ser Ser Asn Asn Ser Leu Asn
625 630 635 640
Tyr Asn Ser Phe Gly Tyr Tyr Thr Leu Ser Gly Leu Glu Leu Glu Ile
645 650 655
Leu Pro Gly Tyr Glu Gln Thr Pro Val Leu His Leu Asp His Trp Ser
660 665 670
Gly Gly Asp Ile Ile Leu Asp Lys Ile Glu Phe Ile Pro Ile
675 680 685
<210> 84
<211> 609
<212> PRT
<213> 芽孢杆菌属的种
<400> 84
Met Lys Asn Lys Pro Val Lys Lys Ala Ile Ala Gly Thr Phe Ile Val
1 5 10 15
Thr Gly Leu Leu Ser Gly Ala Ser Gly Ile Ile Leu Pro Ala Ser Lys
20 25 30
Ala Phe Ala Asp Gln Thr Ser Arg Leu Asp Asp Phe Ile Lys Asn Asp
35 40 45
Glu Ala Gly His Tyr Gly Lys Asn Arg Asp Gly Phe Tyr Ile Lys Ser
50 55 60
Pro Asp Glu Ala Arg Val Leu Pro Gln Gly Gly Ser Trp Phe Thr His
65 70 75 80
Asp Thr His Tyr Arg Asp Ala Glu Ile Ala Pro Phe Phe Leu Ile Asp
85 90 95
Glu Ser Lys Met Asp Ser Ile Ile Lys Glu Val Gln Asn Lys Lys Asp
100 105 110
Met Glu Leu Thr Asp Leu Leu Glu Ile Ile Thr Leu His Ala Pro Ser
115 120 125
Lys Glu Met Asp Gly Glu Asp Asn Asp Ala His Leu Val Arg Tyr Cys
130 135 140
Arg Thr Pro Gly Ile Asp Lys Asp Val Lys Asn His Glu Tyr Leu Thr
145 150 155 160
Val Asp Thr Gly Lys Ser Lys Ile Val Ser Thr Lys Ile Glu Lys Ala
165 170 175
Asp Ala Lys Asp Asp Val Asn Leu Leu Thr Asn Thr Phe Thr Asn Pro
180 185 190
Thr Asp Gly Pro Ile Gln Ala Thr Ser Gly Thr Ile Ser Lys Gln Phe
195 200 205
Thr Asn Thr Ile Ser Thr Thr Gln Thr Asn Ala Thr Thr Leu Gly Met
210 215 220
Lys Gln Thr Leu Lys Thr Gly Ile Asp Phe Phe Asp Ile Ala Lys Glu
225 230 235 240
Asp Phe Ser Leu Glu Phe Asn Glu Ser Tyr Thr Tyr Thr Asn Ala His
245 250 255
Gln Thr Leu Thr Ser Glu Ala Thr Thr Leu Thr Ser Gly Ser Val Ser
260 265 270
Val Asn Ile Lys Pro Gly Lys Ser Tyr Lys Val Gln Thr Met Met Arg
275 280 285
Arg Asp Lys Glu Ser Gly Ile Met Gln Gly Thr Ser Arg Leu Gly Gly
290 295 300
Glu Tyr Thr Ile Ser Asn Gly Asp Tyr Arg Val Asp Leu Asn Ile Tyr
305 310 315 320
Lys Lys Phe Lys Val Ile Gln Asp Phe Tyr Pro Lys Leu Trp Ala Val
325 330 335
Leu Lys Asp Arg Gly Ile Asp Leu Asp Asp Asn Thr Gln Glu Val Leu
340 345 350
Tyr Thr Gly Gly Ile Gly Phe Asp Ser Ile Arg Gly Ala Asn Ile Ile
355 360 365
Thr Thr Ile Thr Asp Leu Asp Thr Gly Asn Lys Ile Glu Lys Pro Val
370 375 380
Ser Gln Val Lys Ala Thr Gly Thr Ile Asn Leu Asp Gly Pro Ile Gln
385 390 395 400
Ala Ser Ile Gln Thr Gly Ile Ala Glu Leu Glu Lys Asn Ser Leu Lys
405 410 415
Leu Gln Thr Ile Asp Gly Val Thr Tyr Cys Val Asp Lys Asp Gly Asn
420 425 430
His Leu Thr Gly Trp Gln Thr Val Ser Gly Thr Gln Tyr Tyr Phe Gly
435 440 445
His Asn Gly Asp Gly Ser Gly Leu Ile His Glu Gly Glu Met Ala Lys
450 455 460
Gly Trp Lys Lys Leu Asp Gly Val Gln Tyr Tyr Phe Asn Lys Thr Gly
465 470 475 480
Asp Gly Ser Gly Met Thr His Glu Gly Glu Met Ala Lys Gly Phe Lys
485 490 495
Thr Ile Asp Gly Val Pro Tyr Tyr Phe Gly Gln Thr Gly Asp Gly Ala
500 505 510
Gly Met Asn Tyr Glu Gly Glu Met Ala Lys Gly Trp Lys Thr Ile Asp
515 520 525
Gly Lys Thr Tyr Tyr Phe Gly Gln Thr Gly Asp Gly Ala Gly Met Asn
530 535 540
His Glu Gly Gln Met Ala Thr Asp Trp Thr Thr Ile Asn Gly Val Lys
545 550 555 560
Tyr Tyr Phe Gly Lys Ala Gly Asp Gly Ile Ala Ser Leu Ala Val Gly
565 570 575
Glu Arg Ala Thr Gly Ser Met Met Ile Asp Gly Gln Val His Tyr Phe
580 585 590
Asn Lys Thr Gly Asp Gly Thr Gly Tyr His His Glu Gly Glu Leu Glu
595 600 605
Trp
<210> 85
<211> 621
<212> PRT
<213> 芽孢杆菌属的种
<400> 85
Met Lys Tyr Lys Asp Arg Thr His Ala Lys Arg Lys Tyr Lys Gln Ala
1 5 10 15
Leu Leu Ala Thr Val Ala Thr Met Thr Leu Gly Val Ser Thr Leu Gly
20 25 30
Ser Thr Ala Thr Ala Phe Ala Asp Glu Lys Gln Thr Glu Val Gln Gln
35 40 45
Gln Gln Ala Asp Ala Pro Pro Ser Thr Gly Pro Val Phe Lys Lys Glu
50 55 60
Lys Asp Gly Thr Phe Thr Val Leu Lys Lys Asn Ile Phe Asn Met Ala
65 70 75 80
Thr Leu Lys Ala Ile Gly Ser Leu Gly Gly Ala Gly Leu Lys Gln Ala
85 90 95
Tyr Ala Asp Tyr Gln Val Asp Gly Gly Asn Phe Asn Asn Thr Phe Arg
100 105 110
Thr Leu Ala Met Gly Ser Ala Ala Leu Ile Pro Tyr Gly Gly Val Val
115 120 125
Leu Ser Pro Leu Ile Gly Leu Leu Trp Ser Glu Asp Lys Thr Ala Glu
130 135 140
Ala Asn Gln Leu Lys Lys Leu Met Glu Ser Ile Ala Ala Gln Thr His
145 150 155 160
Glu Gln Ile Ala Lys Tyr Asp Thr Ala Lys Leu Ser Lys Ala Leu Gln
165 170 175
Asp Leu His Glu Lys Val Glu Glu Phe Glu Asn Leu Val Pro Ile Pro
180 185 190
Gly Ser Gly Ile Phe Tyr Ser Gly Asp Gln Ile His Lys Tyr Glu Asn
195 200 205
Ile Gln Ile Ser Ala Lys Asn Leu Asn Asn Tyr Phe Arg Ser Ala Ile
210 215 220
Ala Ala Cys Gln Thr Glu Gly Tyr Lys Glu Ser Glu Leu Pro Ile Tyr
225 230 235 240
Ile Ile Leu Ala Thr Met His Met Asn Phe Met Asn Tyr Val Ile Lys
245 250 255
Asn Gly Glu Asn Leu Leu His Phe Asp Pro Glu Val Leu Lys Ser Glu
260 265 270
Phe Thr Asp Lys Gln Asp Gly Tyr Ile His Gly Val His Asn Val Pro
275 280 285
Gly Gln Ser Glu Lys Val Gly Tyr Ile Glu Tyr Ile His Asn Phe Ala
290 295 300
Lys Lys Asp Leu Asp Leu Thr Ser Asn Phe Glu Arg Phe Leu Leu Asp
305 310 315 320
Tyr Lys Phe Lys Leu Asp His Asp Lys Ala Leu Ser Ile Gly Ser Ser
325 330 335
Pro Ala Lys Leu Ile Ser Ser Ile Val Met Asn His Asn Asn Lys Leu
340 345 350
Glu Lys Thr Leu Asn Asn Ser Ile Asn Asn Ile Ala Phe Lys Gln Ala
355 360 365
Ala Asn Ile Lys Ser Glu Phe Thr Leu Asp Gln Asn Gly Arg Pro Leu
370 375 380
Tyr Tyr Ser Leu Asp Gly Lys Met Gln Thr Gly Trp Lys Glu Ile Gly
385 390 395 400
Thr Ser Phe Ala Thr Arg His Asp Thr Gly Ser Ser Thr Ala Glu Arg
405 410 415
Leu Phe Ser Arg Glu Trp Leu Lys Ala Gln Gly Ile Gln Tyr Ser Trp
420 425 430
Tyr Tyr Phe Ser Pro Thr Lys Thr Asp Lys Phe Glu Gln Gly Glu Met
435 440 445
Tyr Val Asp Thr Thr Gln Thr Leu Pro Asp Gly Lys Thr Tyr Thr Phe
450 455 460
Asp Ser Asp Gly Lys Cys Leu Asn Pro Asp Gly Gly Ala Val Asn Val
465 470 475 480
Gln Ile Leu Lys Glu Thr Tyr Lys Ile Val Ser Lys Leu Asp Asn Asn
485 490 495
Met Val Leu Asn Thr Ser Asp His Gln Ala Val Leu Lys Gly Thr Asn
500 505 510
Glu Asp Thr Trp Gly Asn Ser Trp Thr Phe Gln Tyr Asp Asn Glu Lys
515 520 525
His Ala Tyr Leu Ile Thr Asn Gly Gln Ser Gly Gly Pro Trp Lys Leu
530 535 540
Ala Glu Tyr Ser Asn Thr Asn Thr Val Phe Ala Thr Asp Asp Lys Ala
545 550 555 560
Asn Lys Gln Glu His Tyr Trp Thr Leu Glu Pro Gly Gly Asp Gly Tyr
565 570 575
Phe Phe Ile His Asn Lys Ser Gln Pro Asp Lys Val Leu Asp Ile Thr
580 585 590
Gly Ser Asn Arg Ala Ala Gly Thr Lys Ile Gly Leu Phe Asn Lys Asn
595 600 605
Gly Gln Asp Asn Gln Lys Phe Lys Leu Glu Lys Ile Asn
610 615 620
<210> 86
<211> 349
<212> PRT
<213> 芽孢杆菌属的种
<400> 86
Met Val Asn Asn Pro Phe Pro Leu Asp Thr Thr Tyr Thr Ile Val Asn
1 5 10 15
Gln Glu Leu Asn Leu Ile Phe Asp Leu Gln Lys Glu Leu Asn Ser Asn
20 25 30
Gly Thr Ser Ile Val Leu Arg Leu Pro Asn Ser Arg Lys Ser Gln Gln
35 40 45
Phe Ile Phe Phe Lys Leu Asp Gly Lys Leu Tyr Ala Ile Ala Asn Gln
50 55 60
Gln Ser Gly Lys Ile Ile Asp Leu Lys Gly Ser Ile Asp Ser Tyr Thr
65 70 75 80
Leu Val Gln Asn Thr Trp Asn Gly Glu Pro Ser Gln Ile Trp Tyr Val
85 90 95
Lys Asn Thr Asn Ile Ser Glu Pro Tyr Tyr Lys Ile Thr Asn Gln Gln
100 105 110
Asn Gly Lys Ile Ala Gln Ala Ile Asn His Ser Asn Asp Thr Asp Asn
115 120 125
Leu Val Ser Leu Thr Ser Trp Asn Asp Gln Lys Met Gln Gln Trp Asn
130 135 140
Phe Thr Pro Lys Lys Ser Ile Lys Phe Pro Lys Asn Ile Glu Ser Lys
145 150 155 160
Pro Leu Pro Phe Pro Pro Asp Tyr Phe Ser Ser Pro Tyr Glu Val Leu
165 170 175
Pro Phe Gln Thr Thr Pro Val Thr Val Ala Ser Ile Leu Leu Pro Cys
180 185 190
Ile Leu Val Thr Asp Asn Glu Trp Asp Asp Gln Thr Lys Ile Gln His
195 200 205
Ser Pro Tyr Tyr Gln Phe Val Lys Glu Gln Tyr Trp Glu Arg Ile Glu
210 215 220
Ser Ile Thr Leu Glu Pro Gly Gln Thr Asn Thr Phe Gln Tyr Lys Thr
225 230 235 240
Gly Met Lys Thr Thr Asp Gln Lys Ser Met Met Asn Thr Ile Gly Met
245 250 255
Ser Ile Gly Ala Asp Leu Gly Phe Gln Phe Asn Gln Thr Ser Thr Lys
260 265 270
Ile Ser Thr Gln Phe Thr Ser Thr Leu Met Val Ser Lys Ser Thr Thr
275 280 285
Ser Glu Gln Leu Thr Glu Thr Thr Ile Thr Asp Ser Leu Thr Asn Thr
290 295 300
Arg Ser Thr Lys Ile Gly Ile Thr Lys Tyr Gln Leu Val Thr Leu Tyr
305 310 315 320
Ser Leu Leu Arg Thr Asp His Ser Phe Val Thr Glu Pro Trp Lys Val
325 330 335
Ser Asp His Leu Thr Thr Lys Asp Arg Thr Ile Ile Ser
340 345
<210> 87
<211> 1047
<212> PRT
<213> 芽孢杆菌属的种
<400> 87
Val His Ile Leu Phe Leu Ser Asp Glu Tyr Val Ser Asn Gly Gly Val
1 5 10 15
Tyr Met Ala His Leu Ser Asp Phe Tyr Pro Ile Ser Tyr Asn Val Leu
20 25 30
Ser Gln Lys Ser Val Tyr Asn Asp Asn Glu Met Tyr Glu Val Gln Gln
35 40 45
Ile Phe Lys Asn Val Leu Thr Ser Trp Glu Gln Tyr Ser Gln Asp Gly
50 55 60
Arg Ile Ser Thr Gln Gln Gln Glu Leu Lys Lys Ala Ile His Asn Leu
65 70 75 80
Lys Lys Tyr Asn Leu Asp Leu Phe Asp Leu Phe Ile Asn Ile Leu Pro
85 90 95
Leu Gly Ala Thr Ile Ser Pro Leu Pro Pro Val His Ser Asn Asp Val
100 105 110
Met Ile Ser Thr Ile Ile Pro Asn Ile Cys Phe Ile Val Asp Ile Val
115 120 125
Trp Pro Gln Ile Pro Lys Gly Leu Asn Thr Gln Leu Asn Met Trp Glu
130 135 140
Val Ile Lys Asp Arg Val Lys Lys Ile Thr Asn Gln Lys Ile Glu Ile
145 150 155 160
Asn Asn Tyr Glu Gln Ile Leu Thr Thr Leu Asn Gln Val Lys Ile His
165 170 175
Leu Leu Asp Leu Lys Asn Ala Ile Gln Thr Thr Val Asp Gln Ser Leu
180 185 190
Ser Tyr Gln Asn Ile Thr Ala Asp Asn Asn Val Tyr His Pro Tyr Lys
195 200 205
Asp Gln Glu Phe Asn Ala Ile Ile Arg Ile Gln Leu Ala Asn Val Leu
210 215 220
Ala Gln Phe Asp Gln Tyr Ile Ser Ile Phe Gln Met Asn Asn Cys Val
225 230 235 240
Ile Ile Glu Leu Pro Tyr Phe Cys Leu Leu Ala Thr Leu His Leu Phe
245 250 255
Thr Leu Gln Ser Val Val His Phe Gly Ser Gln Trp Gly Tyr Ser Thr
260 265 270
Met Gln Ile Lys Asp Tyr Ser Lys Lys Leu Gln Gln Lys Ile Lys Glu
275 280 285
Tyr Thr Asn His Val Tyr Ser Ile Cys Ile Asn Pro Ser His Ile His
290 295 300
Arg Leu Asn Asp Ser Lys Asn Ser Leu Tyr Lys Leu Asp Ser Leu Phe
305 310 315 320
Ser Asn Met Ile Asn Glu Cys Cys Asn Tyr Ile Ala Ile Trp Pro Leu
325 330 335
Leu Asp Pro Val Asp Tyr Pro Ile Arg Thr His Leu Glu Gln Ile His
340 345 350
Ile Pro Phe Ser Asn Ile Ile Thr Ser Glu Asn Ala Thr Phe Glu Leu
355 360 365
Asn Asn His Ile Gln Lys Lys Thr Phe Gln Ser His Asn Leu Ser Lys
370 375 380
Lys Thr Asn Leu Asn Lys Ile Ala Thr Ile Gly Glu Val Asp Arg Asn
385 390 395 400
Ser Gly Val Ile Met Ile Ala Gly Val Pro Val Val Ile Gly Ile Thr
405 410 415
Asn Gly Gln Tyr Val Thr Glu His Ile Thr Lys Thr Ser Ala Ile Lys
420 425 430
Leu Ser Lys Asn Gln Ile Ile Glu Leu Pro Phe Phe Asn Leu Thr Thr
435 440 445
Gly Leu Tyr Gln Ile Arg Tyr Arg Leu Ser Thr Thr Glu Ser Gly Phe
450 455 460
Leu His Phe Lys Leu Gly Arg Trp Asp Asn Ile Val Ser Leu Glu Gln
465 470 475 480
Ile Glu Thr Tyr Asn His Ser Thr Leu Asp Phe Gly Gly Lys Pro Gly
485 490 495
Ile Tyr Lys Leu Pro Ser Lys Lys Val Thr Pro Arg Cys Gly Asn Leu
500 505 510
Ser Ser Ile Asp Asn Ile Leu Ala Ile Asn Ser Gly His His Ile Leu
515 520 525
Thr Ile Ser Asn Ile Gly Ser Val Asn Ile Val Val Asp Arg Ile Glu
530 535 540
Phe Ile Tyr Ile Ser Asn Met Asp Ser Pro His Ser Pro Met Val Phe
545 550 555 560
Asn Asn Lys Lys Tyr Leu Glu Lys Ile Lys Arg Gln Val Asn Gln Leu
565 570 575
Phe Thr Asp Thr Leu Lys Asn Lys Leu Lys Lys Glu Ile Ser Asp Tyr
580 585 590
His Ile Asp Gln Ile Ala Leu Lys Ile Asp Lys Leu Ser Asn Lys Ala
595 600 605
Phe Gln Leu Glu Lys Lys Tyr Leu Arg Gln Leu Ile Asn Lys Ala Lys
610 615 620
Arg Leu Ser Lys Glu Arg Asn Lys Leu Val Gly Gly Asn Phe Glu Lys
625 630 635 640
Leu Asp Lys Trp Leu Leu Gly Tyr Asp Val Val Lys Lys Tyr Asn Lys
645 650 655
Lys Ile Cys Lys Gly Glu Tyr Leu Leu Leu Pro Pro Pro Glu Lys Gly
660 665 670
Leu Ser Tyr Ile Tyr Gln Lys Val Asp Glu Ser Lys Leu Lys Pro Tyr
675 680 685
Thr Arg Tyr Ile Ile Ser Gly Tyr Ile Ile Gln Ser Glu Asn Leu Glu
690 695 700
Leu Ile Val Ser Arg Tyr Gly Asn Glu Ile Gln Thr Leu Leu Asn Val
705 710 715 720
Pro His Ile Asn Glu Asn Thr Phe His Asn Gln Lys His Val Asn Ser
725 730 735
His Phe Phe Ser Tyr Thr Ile Asp Val Gly Met Leu His Pro Glu Ile
740 745 750
Asn Leu Gly Ile Glu Leu Gly Leu Arg Ile Val His Pro Tyr Gly Phe
755 760 765
Ala Lys Leu Ser Asn Leu Glu Ile Arg Glu Ser His Ser Leu Thr Asn
770 775 780
Tyr Asn Thr Leu Lys Arg Gln Asp Ser Leu Tyr Val Asn Lys Tyr Gln
785 790 795 800
Asn Gln Asn Arg Gly Leu Tyr Arg Asn Pro Thr Tyr Gln Ile Asn Asn
805 810 815
Met Lys Ile Lys Asp Asn His Ser Leu Thr Met Gln Glu Ile Ile Gln
820 825 830
Lys Glu Thr Ile Trp Lys Asn Lys Phe Asp Asn Glu Asn Lys Lys Phe
835 840 845
Ile Ile Asp Leu Thr Ser Ile Ile Asn Gln Ile Asn Thr Phe Tyr Lys
850 855 860
Asp Leu Asn Trp Asn Asn Thr Ile His Pro His Val Thr Tyr Gln Lys
865 870 875 880
Ile Asn Gln Val Lys Leu Pro Lys Leu Ser Asn Leu Lys His Trp Phe
885 890 895
Met Asn Asp Arg Pro Gly Glu His Tyr Lys Ile Ile Gln Thr Leu Gln
900 905 910
Asn Ala Leu Asp Cys Ala Phe Ile Gln Leu Glu Asn Gln Asn Leu Ile
915 920 925
Arg Asn Gly Gly Phe Thr Asn Gly Leu Glu Asn Trp Thr Ile Gln Gly
930 935 940
Gln Val Lys Ile Val Thr Phe Ile Pro Asn Lys Gln Thr Val Gln Leu
945 950 955 960
Ser Cys Trp Asp Ala Tyr Met Val Gln Thr Ile Asn Ile Lys Glu Phe
965 970 975
Asp Thr Ile His Thr Tyr Lys Leu Arg Val Tyr Gly Lys Gly Thr Gly
980 985 990
Asn Ile Val Ile Gln His Ser Asp Tyr Val Glu Leu Met Ser Phe Ser
995 1000 1005
Thr Asn Glu Phe Ile Phe Lys Glu Leu Leu Phe Ser Leu Lys Thr
1010 1015 1020
Asn Ile Ile Glu Val Leu Ile Gln Ser Glu Gly Pro Lys Phe Phe
1025 1030 1035
Ile Asp Ser Ile Glu Ile Phe Lys Tyr
1040 1045
<210> 88
<211> 548
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 88
Val His Ile Leu Phe Leu Ser Asp Glu Tyr Val Ser Asn Gly Gly Val
1 5 10 15
Tyr Met Ala His Leu Ser Asp Phe Tyr Pro Ile Ser Tyr Asn Val Leu
20 25 30
Ser Gln Lys Ser Val Tyr Asn Asp Asn Glu Met Tyr Glu Val Gln Gln
35 40 45
Ile Phe Lys Asn Val Leu Thr Ser Trp Glu Gln Tyr Ser Gln Asp Gly
50 55 60
Arg Ile Ser Thr Gln Gln Gln Glu Leu Lys Lys Ala Ile His Asn Leu
65 70 75 80
Lys Lys Tyr Asn Leu Asp Leu Phe Asp Leu Phe Ile Asn Ile Leu Pro
85 90 95
Leu Gly Ala Thr Ile Ser Pro Leu Pro Pro Val His Ser Asn Asp Val
100 105 110
Met Ile Ser Thr Ile Ile Pro Asn Ile Cys Phe Ile Val Asp Ile Val
115 120 125
Trp Pro Gln Ile Pro Lys Gly Leu Asn Thr Gln Leu Asn Met Trp Glu
130 135 140
Val Ile Lys Asp Arg Val Lys Lys Ile Thr Asn Gln Lys Ile Glu Ile
145 150 155 160
Asn Asn Tyr Glu Gln Ile Leu Thr Thr Leu Asn Gln Val Lys Ile His
165 170 175
Leu Leu Asp Leu Lys Asn Ala Ile Gln Thr Thr Val Asp Gln Ser Leu
180 185 190
Ser Tyr Gln Asn Ile Thr Ala Asp Asn Asn Val Tyr His Pro Tyr Lys
195 200 205
Asp Gln Glu Phe Asn Ala Ile Ile Arg Ile Gln Leu Ala Asn Val Leu
210 215 220
Ala Gln Phe Asp Gln Tyr Ile Ser Ile Phe Gln Met Asn Asn Cys Val
225 230 235 240
Ile Ile Glu Leu Pro Tyr Phe Cys Leu Leu Ala Thr Leu His Leu Phe
245 250 255
Thr Leu Gln Ser Val Val His Phe Gly Ser Gln Trp Gly Tyr Ser Thr
260 265 270
Met Gln Ile Lys Asp Tyr Ser Lys Lys Leu Gln Gln Lys Ile Lys Glu
275 280 285
Tyr Thr Asn His Val Tyr Ser Ile Cys Ile Asn Pro Ser His Ile His
290 295 300
Arg Leu Asn Asp Ser Lys Asn Ser Leu Tyr Lys Leu Asp Ser Leu Phe
305 310 315 320
Ser Asn Met Ile Asn Glu Cys Cys Asn Tyr Ile Ala Ile Trp Pro Leu
325 330 335
Leu Asp Pro Val Asp Tyr Pro Ile Arg Thr His Leu Glu Gln Ile His
340 345 350
Ile Pro Phe Ser Asn Ile Ile Thr Ser Glu Asn Ala Thr Phe Glu Leu
355 360 365
Asn Asn His Ile Gln Lys Lys Thr Phe Gln Ser His Asn Leu Ser Lys
370 375 380
Lys Thr Asn Leu Asn Lys Ile Ala Thr Ile Gly Glu Val Asp Arg Asn
385 390 395 400
Ser Gly Val Ile Met Ile Ala Gly Val Pro Val Val Ile Gly Ile Thr
405 410 415
Asn Gly Gln Tyr Val Thr Glu His Ile Thr Lys Thr Ser Ala Ile Lys
420 425 430
Leu Ser Lys Asn Gln Ile Ile Glu Leu Pro Phe Phe Asn Leu Thr Thr
435 440 445
Gly Leu Tyr Gln Ile Arg Tyr Arg Leu Ser Thr Thr Glu Ser Gly Phe
450 455 460
Leu His Phe Lys Leu Gly Arg Trp Asp Asn Ile Val Ser Leu Glu Gln
465 470 475 480
Ile Glu Thr Tyr Asn His Ser Thr Leu Asp Phe Gly Gly Lys Pro Gly
485 490 495
Ile Tyr Lys Leu Pro Ser Lys Lys Val Thr Pro Arg Cys Gly Asn Leu
500 505 510
Ser Ser Ile Asp Asn Ile Leu Ala Ile Asn Ser Gly His His Ile Leu
515 520 525
Thr Ile Ser Asn Ile Gly Ser Val Asn Ile Val Val Asp Arg Ile Glu
530 535 540
Phe Ile Tyr Ile
545
<210> 89
<211> 364
<212> PRT
<213> 芽孢杆菌属的种
<400> 89
Val Asn Glu Met Ala Thr Leu Asn Leu Ser Glu Glu Ser Lys Lys Phe
1 5 10 15
Gln Ser Ile Thr Phe Ala Glu Ser Thr Glu Gln Tyr Gly Leu Arg Gln
20 25 30
Tyr Pro Asn Leu Asn Glu Asn Pro Thr Asn Arg Val Phe Asn Asp Ala
35 40 45
Tyr His Lys Glu Leu Ala Asp Gln Tyr Gly Phe Ile Phe Tyr Lys Leu
50 55 60
Asp Ala Asp Asp Gln Phe Ile Ile Ala Asn Arg Asn Thr Gly Glu Val
65 70 75 80
Leu Gln Ala Pro Gly Thr Thr Gly Pro Asp Ser Val Val Leu His Lys
85 90 95
Gln Tyr Asp Ala Ser Ile Gly Gln Lys Ile Thr Lys Lys Gln Leu Thr
100 105 110
Lys Asp Asn Phe Gln Leu Tyr Val Tyr Asn Ser Thr Gly Ile Ile Ser
115 120 125
Ala Pro Tyr Asp Lys Val Gly Glu Trp Val Asn Ile Val Ser Leu Pro
130 135 140
Lys Asn Ser Asn Glu Asn Asn Val His Thr His Leu Lys Asn Arg Thr
145 150 155 160
Asp Ile Lys Phe Glu Ile Asn Ile Pro Ser Leu Ser Pro Thr Thr Lys
165 170 175
Leu Glu Pro Ile Pro Glu Leu Pro Gly Phe Asn Asp Pro Leu Pro Glu
180 185 190
Asn Glu Asp Ala Lys Arg Ala Val Ile Gly Ser Ala Leu Ile Pro Cys
195 200 205
Val Leu Val Asn Asp Ser Ile Ser Leu Ala Asp Lys Ile Lys Glu Tyr
210 215 220
Pro Tyr Tyr Val Leu Glu Tyr Arg Gln Tyr Trp His Leu Leu Trp Ser
225 230 235 240
Asp Val Phe Thr Ala Gly Ser Asn Lys Ile Lys Thr Glu Val Thr Gly
245 250 255
Ile Pro Ser Glu Ala Gln Glu Lys Met Lys Glu Phe Val Gly Ala Ser
260 265 270
Ile Arg Thr Asp Ile Gly Leu Arg Phe Asn Glu Lys Ser Glu Pro Phe
275 280 285
Gly Lys Gln Ile Thr Ser Gly Leu Asn Val Lys His Ser Tyr Asp Tyr
290 295 300
Glu Leu Gly Glu Thr Thr Thr Thr Thr Asn Val Asp Asn Pro Asn Asp
305 310 315 320
Phe Thr Val Arg Tyr Ala Arg Tyr Ala Arg Ala His Glu Phe Val Leu
325 330 335
Lys Arg Ser Asp Gly Gln Val Val Glu Glu Pro Trp Val Ala Ile Asp
340 345 350
Glu Asn Asp Met Tyr Leu Lys Met Tyr Lys Arg Ala
355 360
<210> 90
<211> 372
<212> PRT
<213> 芽孢杆菌属的种
<400> 90
Val Val Gly Glu Arg Glu Met Lys Arg Tyr Lys Lys Ile Leu Met Val
1 5 10 15
Ala Pro Leu Ala Cys Met Leu Gly Thr Gly Val Val Thr Leu Pro Asp
20 25 30
Ala Ala Tyr Ala Ala Glu Ser Leu Ser Ser Pro Val Ser Phe Thr Asn
35 40 45
Ile Tyr Asn Asp Gly Ala Asp Tyr Thr Lys Glu Asn Leu Ala Lys Asp
50 55 60
Leu Arg Lys Arg Ile Leu Trp Gly Ile Glu Glu Thr Pro Glu Leu Arg
65 70 75 80
Gln Lys Phe Lys Leu Ala Asp Asp Glu Ser Ile Gln Arg Glu Asn Pro
85 90 95
Ala Thr Ser Phe Thr Asp Pro Asn Gly Asn Thr Thr Glu Tyr Pro Ala
100 105 110
Leu Tyr Tyr Gly Leu Glu Asp Gln Leu Ser Lys Val Glu Val Gly Val
115 120 125
Val Gly Ser His Phe Glu Val Asn Pro Thr Val Asp Ser Tyr Glu Asp
130 135 140
Leu Gly Gln Thr Asn Leu Leu Thr Ile Asn Asn Asp Asp Gly Thr Val
145 150 155 160
Ala Gln Thr Ala Gln Thr Pro Glu Thr Thr Glu Lys Phe Ser Glu Thr
165 170 175
Met Ser Tyr Ser Asn Ser Thr Gly Met Lys Ile Gly Ile Gly Thr Ser
180 185 190
Thr Thr Ile Lys Ile Lys Ile Pro Phe Asp Leu Gly Glu Ala Ser Gln
195 200 205
Thr Val Asn Met Asn Ser Glu Phe Ser Phe Asn Asn Thr Gln Thr Gln
210 215 220
Thr Ser Thr His Glu Lys Ser Val Thr Phe Lys Ser Gln Pro Val Val
225 230 235 240
Ala Ala Pro Gly Gly Thr Thr Thr Tyr Tyr Gly Thr Ile Lys Arg Ala
245 250 255
Lys Phe Ser Gly Thr Phe Gln Thr Asp Ala Tyr Val Pro Gly Leu Thr
260 265 270
Leu Lys Leu Pro Ile Val Lys Lys Asn Asn Gly Asn Asp Ile Val His
275 280 285
Thr Glu Glu Val Thr Leu Thr Pro Glu Asp Met Tyr Ala Ile Phe Lys
290 295 300
Asn Gly Leu Pro Val Leu Pro Pro Tyr Leu Ser Leu Asp Asp Lys Ile
305 310 315 320
Lys Lys Val Lys Val Asn Asn Ala Ser Phe Thr Phe Asn Gly Glu Gly
325 330 335
Gly Tyr Tyr Ser Ala Val Gln Val Lys Phe Ile Pro Lys Asp Pro Asn
340 345 350
Lys Lys Ala Gln Val Met Pro Tyr Lys Glu Tyr Val Ala Lys Thr Gln
355 360 365
Glu Lys Ser Leu
370
<210> 91
<211> 378
<212> PRT
<213> 芽孢杆菌属的种
<400> 91
Leu Leu Ile Tyr Ile Cys Lys Ile Leu Leu Lys Lys Gly Gly Leu Ile
1 5 10 15
Met Lys Phe Lys Ser Ala Lys Leu Ala Ile Cys Thr Met Ser Ala Ala
20 25 30
Met Ile Ser Thr Ala Val Ile Pro Ser Thr Ala Val Phe Ala Ala Glu
35 40 45
Thr Gln Leu Asn Val Gly Gln Lys Ala Val Thr Pro Asp Val Ser Ser
50 55 60
Tyr Ile Asp Ile Val Gln Asp Arg Met Lys Gln Arg Asp Lys Glu Ser
65 70 75 80
Lys Leu Ser Gly Glu Ala Ile Asn Met Gln Glu Gln Ile Ile Asp Gly
85 90 95
Trp Phe Leu Ser Arg Phe Trp Ile Phe Lys Asp Gln Asp Asn Asn His
100 105 110
Gln Thr Asn Arg Phe Ile Ser Trp Phe Lys Asp Asn Leu Ala Ser Pro
115 120 125
Lys Gly Tyr Asp Gln Ile Ala Glu Gln Met Gly Leu Asn Ile Glu Ala
130 135 140
Leu Asn Asp Met Asp Thr Ser Ser Ile Asn Tyr Thr Ser Lys Ile Gly
145 150 155 160
Asp Thr Ile Tyr Asn Gly Ile Ser Glu Leu Thr Asn Asn Thr Gly Thr
165 170 175
Thr Gln Lys Met Lys Thr Asp Ser Phe Gln Arg Asp Tyr Thr Lys Ser
180 185 190
Glu Thr Thr Ser Val Thr Asn Gly Leu Gln Val Gly Phe Lys Val Leu
195 200 205
Ala Lys Gly Ile Ile Tyr Leu Ala Gly Ala Asp Leu Glu Ala Ser Val
210 215 220
Asn Tyr Asn Leu Ser Thr Thr Thr Ser Leu Thr Asn Thr Val Ser Asp
225 230 235 240
Lys Phe Thr Val Pro Ser Gln Glu Val Thr Leu Pro Pro Gly His Lys
245 250 255
Ala Val Ile Lys His Asn Leu Asn Lys Met Val Tyr Ser Gly Ser His
260 265 270
Asp Leu Lys Gly Asp Leu Lys Val Ser Phe Ser Asp Lys Glu Leu Val
275 280 285
Gln Lys Phe Ile Tyr Pro Asn Tyr Arg Glu Ile Asn Leu Ser Asp Ile
290 295 300
Arg Lys Thr Met Asn Glu Ile Asp Glu Val Asn Asn Val Asn Pro Val
305 310 315 320
Asp Phe Tyr Gln Leu Val Gly Val Lys Asn His Val Lys Asn Gly Asp
325 330 335
Thr Ile Tyr Ile Asp Thr Pro Ala Glu Phe Thr Phe Asn Gly Ala Asn
340 345 350
Pro Tyr Tyr Arg Ala Ile Phe Thr Glu Tyr Asp Asp Asn Gly Asn Pro
355 360 365
Ile Gln Val Lys Ser Leu Gly Gly Asn Leu
370 375
<210> 92
<211> 732
<212> PRT
<213> 芽孢杆菌属的种
<400> 92
Met Asn Asn Asn Gln Met Asn Phe Ser Asn Asp Ile Thr Asp Asn Ser
1 5 10 15
Asn Ile Asn Asn Phe Glu Asn Lys Ile Gln Asn Asn Met Glu Asn Leu
20 25 30
His Tyr Glu Glu Val Ser Asp Ile Val Ser Asn Leu Glu Glu Lys Asn
35 40 45
Gly Tyr Asp Val Pro Ile Glu Asn Ser Ser Ser Ile Arg Asp Phe Glu
50 55 60
Met Thr Arg Glu Ala Phe Glu Pro Tyr Asn Val Leu Ala Gln Pro Leu
65 70 75 80
Asp Thr Arg Glu Leu Asp Glu Leu Arg Arg Thr Trp Glu Asn Trp Glu
85 90 95
Met Glu Val Ser Phe Leu Asp Thr Arg Ile Val Pro Val Val Gly Val
100 105 110
Ile Gly Ser Phe Leu Ala Lys Glu Ile Ala Lys Ile Ala Gly Lys Lys
115 120 125
Leu Leu Lys Ser Leu Leu Gly Phe Leu Phe Pro Asn Asp Asn Thr Glu
130 135 140
Gln Phe Glu Ala Ile Leu Glu Ala Thr Glu Glu Leu Val Asn Arg Lys
145 150 155 160
Val Thr Glu Val Ile Arg Asp Arg Val Gln Gln Glu Leu Val Gly Leu
165 170 175
Gln Gly Asn Ile Glu Val Phe Leu Gln Asp Val Glu Asp Phe Glu Thr
180 185 190
Phe Ser Phe Leu Ser Val Glu Glu Lys Glu Ile Leu Lys Arg Lys Thr
195 200 205
Tyr Leu Arg Thr Gly Asn Thr Asn Ile Glu Pro Lys Ala Ile Ile Asp
210 215 220
Ser Ile Asn Thr Leu Asn Gln Leu Phe Met Ser Arg Met Pro Gln Phe
225 230 235 240
Ser Ser Ser Asn Pro Asp Trp Lys Leu Glu Leu Leu Pro Leu Phe Ala
245 250 255
Gln Ala Ala Asn Leu His Leu Leu Phe Leu Arg Asp Val Ile Asn Asn
260 265 270
Ala Thr Glu Trp Glu Leu Thr Ser Thr Gln Leu Thr Thr Tyr Lys Thr
275 280 285
Arg Leu Lys Ser Lys Val Val Glu Tyr Ser Asn Tyr Cys Leu Glu Thr
290 295 300
Tyr Arg Glu Asn Phe Asn Lys Ile Tyr Asn Thr Leu Ser Leu Lys Lys
305 310 315 320
Val Leu Asn Phe Arg Asn Tyr Met Val Phe Asn Val Leu Asp Tyr Val
325 330 335
Ser Thr Trp Ser Leu Leu Arg Tyr Ser Asn Val Asn Ile Asn Ser Ser
340 345 350
Thr Asn Ile Tyr His Leu Glu Lys Asp Ile Thr Lys Tyr Ala Ser Trp
355 360 365
Ser Ile Val Asn Gln Phe Ile Gln Gly Lys Pro Phe Lys Ile Phe Ser
370 375 380
Gly Leu Ser Ser Lys Ala Thr Ser Val Thr Phe Arg Gly Ser Asn Gln
385 390 395 400
Ile Asn Leu Pro Ala His His Met Asn Leu Ile Asp Asn Val Thr Thr
405 410 415
His Tyr Lys Gly Gly Thr Ser Ile Gly Pro Tyr Gly Ala Leu Pro Thr
420 425 430
Ala Asn Pro Gly Asn Asp Ile Ser Tyr Gly Phe Lys Asn Tyr Ser Gln
435 440 445
Ser Thr Ser Leu Ser Asn Pro Ile Asp Asn Pro Phe Tyr Lys Val Val
450 455 460
Gln Glu Ile Gln Ile Thr Lys Pro Ser Ser Lys Arg Ile Glu Thr Lys
465 470 475 480
Glu Tyr Ala Met Tyr Gln Ala Val Asn Asn Pro Thr Gly Gln Leu Thr
485 490 495
Asp Ile Tyr Gly Gly Asn Trp Val Ser Phe Pro Glu Tyr Thr Val Lys
500 505 510
His Val Met Glu Leu Pro Val Asp Asn Asp Phe Ser Leu Leu Pro Ala
515 520 525
Val Lys Ser Asn Ile Ser Leu Leu Asn His His Pro Leu Pro Pro Tyr
530 535 540
Lys Tyr Ser Arg Pro Gly Gly Leu Ile Ala Ile Phe Asn Arg Asp Thr
545 550 555 560
Pro Ile Lys Pro Tyr Thr Asn Thr Gly Asn Phe Thr Phe Lys His Thr
565 570 575
Val Pro Ser Asp Gly Ala Gly Phe Thr Ile Ser Pro Leu Gln Phe His
580 585 590
Ser Leu Thr Ala Asn Tyr Gln Pro Asn Ile Asp Arg Ala Cys Ile Gln
595 600 605
Glu Leu Leu Gly Asn His Gly Asp Gly Val Leu Phe Pro Glu Thr Lys
610 615 620
Ala Thr Tyr Thr Arg Tyr Asn Tyr Ala Ile Tyr Asn Pro Thr Ser Thr
625 630 635 640
Ala Arg Gln Tyr Arg Ile Tyr Leu Lys Leu Ala Thr Pro Asn Gly Ala
645 650 655
Ala Lys Phe Trp Leu Val Phe Asn Asn Gly Gly Ser Val Arg Tyr Asp
660 665 670
Val Asp Thr Leu Asn Gln Asn Asp Gly Ile Asn Asp His Gly Ala Ala
675 680 685
Phe His Glu Ala His Tyr Asn Thr Ile Ile Ile Pro Ala Gly Glu Thr
690 695 700
Tyr Val Leu Gly Val Glu His Gln Gly Pro Ala Ser Ile Leu Gly Arg
705 710 715 720
Ile Met Leu Thr Pro Thr Asn Val Thr Pro Ile Tyr
725 730
<210> 93
<211> 187
<212> PRT
<213> 芽孢杆菌属的种
<400> 93
Val Asn Glu Tyr Ile Met Thr Asn His Ser Asn Ser Ser Gly Tyr Ser
1 5 10 15
Asp Asp Gly Val Ile Thr Ser Asn His Thr Met Thr Asp Asn Tyr Ser
20 25 30
Gly Asp Ser Leu Thr Pro Asn Asn Lys Ile Pro Phe Ser Phe Phe Val
35 40 45
Gln Leu Pro Asn Glu Phe Val Tyr His Thr Lys Gln Gln Thr Lys Val
50 55 60
Val Tyr Asn Val Lys Tyr Leu Thr Leu Ser Gln Glu Met Val Lys His
65 70 75 80
Pro Ile Gln Val Asn Gln Leu Gly Asn Ile Asp Ile Asp Leu Gln Val
85 90 95
Leu Lys Val Lys Gly Cys Leu Pro Ile Tyr Phe Asn Ile Pro Ile Thr
100 105 110
Pro Ile Ala Pro Glu His Met Cys Thr Ile His Arg Glu Phe Pro Leu
115 120 125
Leu Tyr Met Gly Tyr Gln Asp Ile Ile Ser Val Asn His Ile Ile Lys
130 135 140
Cys Ser Thr Gly Thr Leu Pro Tyr Tyr Leu Leu Asp Cys Glu His Ile
145 150 155 160
Arg Ile Cys Asp Leu Gln Val Lys Ala Gly Glu Asn Glu Gln Ser Val
165 170 175
Lys Val Ser Gly Glu Phe Glu Leu Leu Tyr Thr
180 185
<210> 94
<211> 395
<212> PRT
<213> 芽孢杆菌属的种
<400> 94
Val Tyr Gln Val Lys Glu Ile Arg Phe Lys Ile Gly Tyr Asn Ser Phe
1 5 10 15
Ser Phe Cys Ile Glu Glu Arg Tyr Met Met Ser Asn Glu Asn Asn Asn
20 25 30
Arg Arg Asn Met Asn Ala Ser Met Gly Ser Asp Gly Glu Ser Ile Gln
35 40 45
Asn Lys Ile Gly Asp Ser Arg Ser Gln Ile Ser Phe Pro Asp Phe Asn
50 55 60
Phe Tyr Lys Ile Arg Thr Phe Ser Gly Lys Tyr Ala Asp Ile Thr Gly
65 70 75 80
Asp Ser Leu Leu Val Gln Tyr Asn Gly His Asn Gly Asp Asn Gln Lys
85 90 95
Phe Leu Ile Val Thr Leu Asp Asn Asp Tyr Ser Ile Ile Ala Ala Lys
100 105 110
His Thr Gly Lys Val Met His Glu Phe Tyr Leu Phe Gly Thr Gly Arg
115 120 125
Phe Leu Gln Ala Asp Phe His Asn Met Asp Glu Gln Lys Phe Phe Ile
130 135 140
Ser Asp Asp Gly Thr Ile Ala Val Lys Lys Asp Arg Lys Val Trp Asp
145 150 155 160
Ala Thr Gly Gly Thr Ser Asn Gly Ser Pro Ile Ile Pro Tyr Asp Tyr
165 170 175
His Gly Ala Ser Asn Gln Lys Phe Thr Leu Glu Arg Ser Gly Ser Val
180 185 190
Ser Val Thr Lys Pro Thr Ile Gly Thr Leu Pro Pro Ala Pro Asp Phe
195 200 205
Lys Thr Thr Asp Leu Asn Glu Ile Leu Pro Asp Asn Thr Glu Pro Val
210 215 220
Ile Thr His Ala Thr Tyr Leu Pro Tyr Phe Met Val Lys Asp Pro Tyr
225 230 235 240
Tyr Asn Asn Gln Gln Lys Met Gln Asn Ser Pro Tyr Tyr Ile Leu Val
245 250 255
Arg Arg Gln Tyr Trp Glu Lys Ile Thr Gln Arg Ile Val Gly Ala Gly
260 265 270
Glu Ser Tyr Lys Tyr Glu Gln Lys Thr Gly Val Ser Arg Thr Asp Gln
275 280 285
Thr Ser Met Thr Glu Thr Thr Glu Ile Ala Ile Gly Ala Asp Met Gly
290 295 300
Phe Met Phe Lys Gly Phe Ser Val Gly Leu Ser Thr Ser Ile Thr Lys
305 310 315 320
Thr Leu Ser Val Thr Lys Ser Thr Ser Thr Thr Glu Ser Thr Glu Thr
325 330 335
Thr Glu Ile Val Thr Asp Ser Asn Pro Phe Met His Thr Ile Ala Arg
340 345 350
Ala Lys Tyr Met Leu Ile Asn Glu Tyr Tyr Val Thr Arg Ala Asn Gly
355 360 365
Glu Lys Ile Thr Ser Gly Asp Ala Ala Tyr Trp Lys Val Pro Asn Pro
370 375 380
Ser Gln Thr Ala Thr Arg Thr Ile Pro Arg Thr
385 390 395
<210> 95
<211> 246
<212> PRT
<213> 芽孢杆菌属的种
<400> 95
Met Asn Gln Ser Leu Thr Asn Leu Lys Lys Ile Lys Phe Gly Pro Ile
1 5 10 15
Phe Lys Lys Ala Ser Leu Leu Asp Glu Asn Gln Pro Ile Ile Gly Ile
20 25 30
Asp Ile Glu Glu Glu Ile Asp Pro Lys Tyr Thr Pro Gln Gly Met Gly
35 40 45
Ile Met Lys Ser Phe Ser Asp Tyr Tyr Glu Ile Trp Gln Asp Val Glu
50 55 60
Arg Asn Asn Ala Glu Ile Val Ile Phe Asp Glu Ala Lys Ala Phe Ala
65 70 75 80
Asn Leu Ser Ser Phe Pro Gly Lys Gly Thr Ala Gly Tyr His Gly Tyr
85 90 95
Asn Ile Trp Lys Asp Ser Lys Glu Ile Val Val Asn Asp Ile Ile Asn
100 105 110
Leu Ile Val Glu Gln Leu Ile Ile Asn Ala Arg Ile Gly Leu Leu Ser
115 120 125
Leu Asn Glu Thr Asp Lys Asp Asn Leu Lys Lys Leu Val Ser Arg Val
130 135 140
Phe Thr Glu Val Gly Thr Lys Asp Tyr Lys Arg Ser Trp Ser Val Tyr
145 150 155 160
Asn Asn Phe Ser Asp Asn Ser Val Val Tyr Ser Tyr Ser Met Gly Leu
165 170 175
Leu Lys Gln Asn Leu Thr Thr Lys Pro Tyr Ile Tyr Ala Met Pro Leu
180 185 190
Gly Phe Gln Ile Arg Val Asn Lys Asn Met Lys Gln Leu Phe Gly Trp
195 200 205
Asn Leu Asn Ser Lys Ala Ala Tyr Asp Val Lys Val Val Ser Met Val
210 215 220
Tyr Arg Glu Thr Ile Asn Pro Asn Leu Ser Gln Met Ser Phe Glu Glu
225 230 235 240
Tyr Leu Glu Ile Leu Phe
245
<210> 96
<211> 241
<212> PRT
<213> 芽孢杆菌属的种
<400> 96
Val Arg Asn Tyr Thr Ser Glu Lys Gln Ser Gln Thr Val Leu Phe Ser
1 5 10 15
Glu Lys Ser Ile Glu Thr Phe Ser Arg Ser Thr Thr Glu Gly Tyr Lys
20 25 30
Val Gly Ser Ser Ile Lys Ser Thr Thr Ser Phe Lys Val Lys Val Gly
35 40 45
Phe Leu Val Ser Gly Glu Ile Ser Glu Ser Ile Glu Val Ala Val Thr
50 55 60
Gly Glu Tyr Asn His Ser Ser Thr Glu Thr Thr Thr Thr Thr Thr Thr
65 70 75 80
Thr Thr Glu Lys Leu Trp Gln Val Thr Gln Pro Val Ile Ile Pro Pro
85 90 95
His Thr Gln Ile Thr Ala Thr Leu Gln Ile Phe Ser Gly Pro Phe Val
100 105 110
Val Pro Ala Lys Thr Lys Ala Thr Ile Gln Gly Lys Gly Thr Ile Asn
115 120 125
Gly Thr Pro Asn Trp Ala Ser Phe Ile Asn Tyr Thr Lys Tyr Asp Gly
130 135 140
Arg Tyr Trp Ala Ala Tyr Asn Arg Ala Ser Gly Leu Tyr Asn Asp Arg
145 150 155 160
Asn Ser Trp Pro Gly Tyr Lys Lys Ile Tyr Val Gly Thr Gly Ser Gly
165 170 175
Thr Gly Ser Leu Arg Leu Glu Gly Glu Ala Arg Ile Asn Ala Gln Val
180 185 190
Gly Leu Tyr Ala Val Thr Glu Phe Lys Glu Ser Pro Leu Pro Gly Tyr
195 200 205
Glu Ser Val Gly Ser Asn Arg Thr Tyr Tyr Ala Pro Asn Ile Ile Leu
210 215 220
Gly Asp Gly Ser Ile Ile Gln Phe Pro Glu Tyr Gln Arg Tyr Leu Gln
225 230 235 240
Arg
<210> 97
<211> 553
<212> PRT
<213> 芽孢杆菌属的种
<400> 97
Met Asn Tyr Lys Asn Arg Thr Asn Ala Lys Arg Lys Tyr Lys Gln Thr
1 5 10 15
Leu Phe Val Thr Ile Ala Thr Met Thr Leu Gly Gly Ser Ile Leu Gly
20 25 30
Asn Thr Thr Ser Ala Phe Ser Ala Glu Asn Thr Ala Glu Lys Gln Lys
35 40 45
Val Ser Ser Tyr Leu Lys Asn Ser Asp Thr Val Gly Ser Asn Val Phe
50 55 60
Lys Val Ala Ala Asp Gly Ser Val Thr Leu Leu Asn Ser Asn Pro Phe
65 70 75 80
Asp Ser Pro Thr Val Lys Ala Ile Thr Leu Leu Gly Gly Asn Leu Leu
85 90 95
Asn Gln Ala Tyr Leu Asp Ala Tyr Lys Lys Asp Phe Thr Gly Thr Ala
100 105 110
Arg Thr Leu Val Leu Gly Ala Thr Ala Leu Ile Pro Tyr Val Gly Ser
115 120 125
Phe Ile Ser Pro Val Ile Gly Leu Leu Trp Pro Ala Asn Ile Gly Thr
130 135 140
Asp Met Thr Ala Gln Thr Lys Ala Leu Val Ala Leu Ile Asn Glu Gln
145 150 155 160
Ile Ala Asp Tyr Asp Ile Glu Thr Leu Glu Gln Lys Gly Lys Thr Leu
165 170 175
Lys Asp Leu Met Ser Arg Phe Asp Asp Leu Leu Asn Gly Lys Pro Leu
180 185 190
Thr Phe Gly Glu Gln Gly Thr Ile Gln Glu Thr Leu Arg Asn Lys Ala
195 200 205
Ser Asn Ile Asn Asp Lys Phe Ile Glu Val Ile Asn Asp Cys Gln Lys
210 215 220
Arg Ser Phe Lys Ala Glu Glu Leu Pro Ile Tyr Thr Leu Ile Ala Thr
225 230 235 240
Ala His Leu Leu Phe Leu Asn Met Ile Glu Gln Asn Gly Gln Ser Ser
245 250 255
Arg Ile Gln Met Asp Asp Glu Thr Tyr Lys Gly Tyr Val Glu Asp Phe
260 265 270
Lys Arg Leu Pro Ile Glu Tyr Ala Asn Tyr Ile Glu Ala Thr Ser Lys
275 280 285
Leu Gly Lys Gln Lys Phe Asn Thr Lys Met Asn Asp Thr Ile Asn Ala
290 295 300
Ala Lys Lys Gln Ala Leu Val Gly Ile Ile Ser Ser Asp Glu Lys Thr
305 310 315 320
Asn Leu Glu Asn Leu Glu Lys Thr Arg Lys Gln Cys Leu Asp Glu Ala
325 330 335
Lys Leu Leu Gln Gln Met Gly Asp Ala Tyr Gly Ala Glu Lys Ala Arg
340 345 350
Gln Lys Ala Gln Lys Ala Tyr Glu Tyr Lys Lys Gln Tyr Glu Asp Leu
355 360 365
Ile Gln Lys Arg Asp Asn Tyr Ile Lys Asn Thr Ala Asp Asn Glu Ala
370 375 380
Phe Lys Leu Ala Lys Thr Ser Met Glu Glu Leu Met Ile Asp Val Met
385 390 395 400
Ile Pro Asn Gly Lys Tyr Ile Ile Gln Ser Val Gln Asn Ser Asn Met
405 410 415
Val Val Gly Met Ser Gly Asn Asn Ile Gln Leu Glu Asp Asn Asn Gly
420 425 430
Gln Leu Asn Asn Lys Phe Val Ile Ile Tyr Asp Gln Ala Arg Asn Ser
435 440 445
Tyr Val Ile Glu Thr Ser Val Glu Thr Gly Leu Ser Ser Tyr Met Ala
450 455 460
Ala Asn Ile Asp Tyr Ser Asn Val Asn Lys Ser Gly Val Asn Val Tyr
465 470 475 480
Ala Ile Pro Ser Ile Ser Ser His Glu Gln Pro Asp Ile Leu His Trp
485 490 495
Lys Leu Lys Arg Ala Arg Gly Ser Lys Phe Val Tyr Leu Glu Asn Thr
500 505 510
Ala Asn Gly Gln Val Leu Asp Val Ser Asn Ala Gly Thr Glu Lys Gly
515 520 525
Thr Asn Ile Ile Thr Trp Pro Leu Lys Gln Gln Gly Gln Asn Gln Asn
530 535 540
Gln Gly Phe Lys Leu Val Pro Thr Asn
545 550
<210> 98
<211> 287
<212> PRT
<213> 芽孢杆菌属的种
<400> 98
Met Asn His Leu Ala Phe Ser Pro Arg Glu Leu Gly Gly Lys Asp Ser
1 5 10 15
Pro Phe Leu Leu Thr Ile Glu Glu Trp Arg Ser Phe Ala Asn Tyr Leu
20 25 30
Asn Lys Trp Ile Ser Ala Pro Thr Ser Ile Asp Gln Val Lys Glu Arg
35 40 45
Ile Tyr Leu Ile Leu Asp Gly Lys Val Lys Ser Asn Trp Asp Arg Phe
50 55 60
Ile Thr Thr Ser Thr Phe Arg Arg Leu Val Glu Glu Ala Leu Ala Thr
65 70 75 80
Lys Leu Asn Val Arg Glu Lys Cys Arg Phe Trp Asn Asn Gly Gly His
85 90 95
Lys Asp Ile Leu Ile Leu Ser Gln Asn Ile Val Gly Phe Ala Asp Leu
100 105 110
Ile Thr Ile Lys Tyr Phe Asn Asp Leu Asn Gln Val Ile Leu Glu Ala
115 120 125
Gln Asn Gly Lys Leu Ser Pro Ser Thr Lys Ser Lys Phe Val Asn Met
130 135 140
Cys Lys Asp Leu Ser Asn His Ala Gln Thr Phe Tyr Gln Gln Ser Gln
145 150 155 160
Ser Ile Ala Thr Ser Leu Ser Glu Phe Tyr Ser Glu Ile Gln Lys Tyr
165 170 175
Glu Glu Ile Val Pro Thr Trp Ser Tyr Leu Met Ser Gln Leu Pro Leu
180 185 190
Tyr Asn Ser Asn Asp Phe Val Val Ala Gln Asn Thr Val Val Tyr Leu
195 200 205
Val Ala Pro Val Lys His Leu Ile Gln Phe Lys Glu Asn Val Leu Pro
210 215 220
Val Ile Arg Lys Val His Arg Ile Trp Gly Ser Ile Ser Tyr Asp Leu
225 230 235 240
Lys Glu Leu Ala Asp Asn Ile Glu Asp Ile Glu Asn Leu Glu Glu Phe
245 250 255
Ile Ala Ala Leu Glu Leu Glu Leu Ala Phe Glu Asp Trp Lys Ala Ile
260 265 270
Arg Asp Glu Ala Glu Asn Phe Tyr Lys Asn Ala Glu Asn Phe Ser
275 280 285
<210> 99
<211> 1261
<212> PRT
<213> 芽孢杆菌属的种
<400> 99
Met Asn Gln Asp Tyr Asn Asn Lys Lys Ser Glu Met Met Asp Thr Asp
1 5 10 15
Val Ile Asn Tyr Gln Thr Arg Tyr Pro Leu Ala Lys Glu Pro Gly Arg
20 25 30
Ile Phe Gln Glu Glu Asn Asn Lys Asp Cys Asn Val Met Tyr Asn Arg
35 40 45
Asn Pro Gly Thr Phe Gly Pro Val Asn Thr Ala Gly Lys Val Ala Ser
50 55 60
Gly Ile Ile Gly Ser Val Met Arg Ser Phe Lys Arg Gln Lys Gln Ile
65 70 75 80
Asp Gln Ile Val Asp Ile Val Leu Ile Glu Val Tyr Ala Thr Leu Trp
85 90 95
Pro Ile Leu Glu Gly Met Trp Tyr Ala Met Met Asp Ala Val Glu Ile
100 105 110
Met Ile Gln Glu Ala Ile Thr Thr Ala Val Arg Ser Lys Ala Gln Ala
115 120 125
Glu Leu Asp Gly Val Arg Asn Ala Leu Ala Leu Tyr Gln Gln Ala Phe
130 135 140
Asp Asp Trp Glu Lys Asp Pro Asp Asn Leu Gln Leu Gln Glu Arg Ile
145 150 155 160
Arg Arg Gln Phe Thr Thr Thr Asn Thr Ile Ile Glu Phe Ala Met Pro
165 170 175
Ser Phe Ala Val Ser Gly Phe Glu Val Pro Leu Leu Val Val Tyr Ala
180 185 190
Gln Ala Ala Asn Leu His Leu Leu Phe Leu Arg Ala Ala Val Val Phe
195 200 205
Gly Glu Lys Trp Gly Met Ser Arg Glu Glu Ile Glu Asp Tyr Tyr Ser
210 215 220
Gly Asn Phe Gly Leu Thr Gly Arg Thr Lys Leu Tyr Thr Asn His Cys
225 230 235 240
Ile Asn Trp Tyr Asp Lys Gly Ile Glu Lys Ala Lys Val Leu Asn Pro
245 250 255
Ser Ala Thr Ile Leu Glu Gln Trp Asn Leu Phe Asn Asp Phe Arg Arg
260 265 270
Glu Met Thr Ile Met Val Leu Asp Ile Val Ala Leu Trp Pro Met Tyr
275 280 285
Asp Val Lys Leu Tyr Pro Glu Gly Ile Thr Thr Glu Leu Thr Arg Thr
290 295 300
Ile Tyr Thr Pro Ser Met Gly Ile Leu Glu Asp Ser Pro Met Met Ser
305 310 315 320
Met Val Arg Glu Asp His Gly Asn Leu Glu Ala Met Ala Ala Gln Glu
325 330 335
Leu Leu Ser Thr Ser Pro Pro Ser Leu Phe Thr Trp Leu Glu Lys Gln
340 345 350
Ile Val Tyr Arg Tyr Lys Asn Ala Ile Tyr Gly Tyr Gln Asn Phe Leu
355 360 365
Arg Thr Thr Phe Gly Glu Glu Phe Ser Ser Gly Ile Ile Gly Ala Thr
370 375 380
Asp Ser Pro Tyr Thr Ser Asn Pro Lys Ser Ile Pro Ile Gly Ser Glu
385 390 395 400
Leu Tyr Asp Val Tyr Lys Val Val Thr Ser Tyr Leu Glu Val Asp Pro
405 410 415
Tyr Pro Asn Lys Asp Ser Pro Ile His Arg Leu Val Tyr Tyr Arg Leu
420 425 430
Lys Asp Glu Gln Glu Ser Ile Ile Arg Thr Asp Thr Ser Glu Gly Pro
435 440 445
Ile Asp Gln Val Ser Glu Ile Thr Tyr Asp Glu Glu Phe Pro Glu Asn
450 455 460
Arg His Arg Leu Ala Tyr Met Ser Gly Leu Gln Ser Phe Phe Ser Leu
465 470 475 480
Ala Trp Thr Thr Glu Gly Leu Phe Asn Ser Tyr Trp Ala Pro Tyr Asn
485 490 495
Ile Thr Leu Gly Trp Thr Tyr Ala Asp Val Asp Arg Thr Asn Thr Ile
500 505 510
Ala Pro Asp Val Ile Thr Gln Ile Pro Ala Val Lys Gly Tyr Lys Ile
515 520 525
Ser Gly Asn Pro Tyr Leu Glu Gly Ala Glu Gly Ile Gly Ala Val Pro
530 535 540
Gly Pro Gly Phe Thr Gly Gly Asn Leu Val Gly Leu Ala Ala Gly Ala
545 550 555 560
Lys Leu Tyr Met Lys Val Thr Asn Pro Val Ser Asn Leu Val Gly Tyr
565 570 575
His Ile Arg Ile Arg Tyr Ala Asn Asn Gln Pro Thr Val Leu Ser Ile
580 585 590
Ser Tyr Gln Gly Leu Glu Thr Ser Ser Gly Arg Phe Asp Ile Pro Val
595 600 605
Thr Tyr Ser Gly Asp Tyr Thr Ala Lys Leu Thr Tyr Glu Ala Phe His
610 615 620
Tyr Lys Glu Thr Ile Val Ile Ser Ser Pro Ile Gly Glu Glu Val Ala
625 630 635 640
Glu Ile Met Leu His Asn Glu Gly Arg Ser His Leu Leu Ile Asp Lys
645 650 655
Ile Glu Phe Ile Pro Ile Glu Gly Ser Leu Glu Glu Tyr Lys Val Asn
660 665 670
Gln Ala Leu Glu Lys Ala Arg Lys Ala Val Asn Val Leu Phe Thr Ser
675 680 685
Asp Ala Lys Asn Ala Leu Lys Leu Asn Ile Thr Asp Tyr Val Val Asp
690 695 700
Gln Ala Ala Asn Leu Val Glu Cys Val Ser Glu Asp Phe His Ala Lys
705 710 715 720
Glu Lys Met Ile Leu Leu Asp Gln Val Lys Phe Ala Lys Arg Leu Ser
725 730 735
Gln Ala Arg Asn Leu Leu Asn Tyr Gly Asp Phe Glu Ser Ser Asp Trp
740 745 750
Ser Gly Glu Asn Gly Trp Arg Thr Ser Asn His Val His Val Ala Val
755 760 765
Asp Asn Pro Thr Phe Lys Gly Arg Tyr Leu His Met Pro Gly Ala Met
770 775 780
Ser Pro Gln Phe Ser Asn Asn Ile Tyr Pro Thr Tyr Val Tyr Gln Lys
785 790 795 800
Val Asp Glu Ser Lys Leu Lys Ser Tyr Thr Arg Tyr Leu Val Arg Gly
805 810 815
Phe Val Gly Asn Ser Lys Asp Leu Glu Leu Leu Val Glu Arg Tyr Gly
820 825 830
Lys Asp Val His Val Glu Met Asn Val Pro Asn Asp Ile Arg Tyr Ser
835 840 845
Leu Ser Met Asn Glu Cys Gly Gly Phe Asp Arg Cys Lys Pro Val Ser
850 855 860
Tyr Glu Thr Ser Pro Ser His Thr Cys Thr Cys Lys Asp Ile Ala Val
865 870 875 880
Ala His Thr Asp Cys Gln Cys Lys Asp Lys Gly Asn Arg Ile Ser Thr
885 890 895
Asn Met Tyr Thr Asn Val Pro Thr Asp Ser Ala Val Tyr Thr Asn Gly
900 905 910
Ser His Ala His Lys Ser Cys Gly Cys Lys His Thr Asn Ser Ser Tyr
915 920 925
Pro Asn Gly Lys His Pro His Lys Pro Cys Gly Cys Gln Asn Pro His
930 935 940
Val Phe Thr Tyr Tyr Ile Asp Thr Gly Cys Val Asp Gln Glu Glu Asn
945 950 955 960
Leu Gly Leu Phe Phe Ala Leu Lys Ile Ala Ser Glu Asn Gly Ile Ala
965 970 975
Asn Ile Asp Asn Leu Glu Ile Ile Glu Ala Gln Pro Leu Thr Gly Glu
980 985 990
Ala Leu Ala Arg Val Lys Lys Arg Glu Gln Lys Trp Lys Gln Glu Arg
995 1000 1005
Asp Lys Lys Arg Leu Glu Thr Glu Lys Ala Val Gln Ala Ala Gln
1010 1015 1020
Glu Ala Ile Gln Gly Leu Phe Thr Asn Thr Gln Gln Asn Arg Leu
1025 1030 1035
Lys Phe Lys Thr Leu Phe Pro Gln Ile Leu Lys Ala Glu Met Leu
1040 1045 1050
Val Gln Gln Ile Pro Tyr Val Tyr His Asp Tyr Leu Leu Gly Ala
1055 1060 1065
Ile Pro Pro Met Leu Gly Met Asn Tyr Glu Ile Tyr Gln Gln Leu
1070 1075 1080
Ala Ala Ser Ile Arg Asn Ala His Thr Leu Tyr Ala Glu Trp Asn
1085 1090 1095
Leu Val Ser Asn Gly Thr Phe Arg Ser Gly Thr Gly Ser Trp Asn
1100 1105 1110
Val Ser Glu Gly Val Lys Val Gln Pro Leu Gln Asn Thr Ser Val
1115 1120 1125
Leu Val Leu Ser Glu Trp Ser His Glu Ala Ser Gln Gln Leu Arg
1130 1135 1140
Ile Asp Pro Asp Arg Gly Tyr Val Leu Arg Val Thr Ala Arg Lys
1145 1150 1155
Glu Gly Ala Gly Lys Gly Thr Val Thr Met Ser Asp Cys Ala Ala
1160 1165 1170
Tyr Thr Glu Thr Leu Thr Phe Thr Ser Cys Asp Tyr Asn Thr Val
1175 1180 1185
Asp Ser Gln Ala Met Thr Gly Asp Thr Leu Ser Gly Phe Val Thr
1190 1195 1200
Lys Thr Leu Glu Ile Phe Pro Asp Thr Asp Arg Ile Arg Ile Asp
1205 1210 1215
Ile Gly Glu Thr Glu Gly Thr Phe Lys Val Glu Ser Val Glu Leu
1220 1225 1230
Ile Cys Met Glu His Met Val Asp Pro Ile Cys Trp Pro Asp Tyr
1235 1240 1245
Asp Cys Pro Phe Pro Ile Glu Pro Pro Ser Val Asn Asp
1250 1255 1260
<210> 100
<211> 662
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 100
Met Asn Gln Asp Tyr Asn Asn Lys Lys Ser Glu Met Met Asp Thr Asp
1 5 10 15
Val Ile Asn Tyr Gln Thr Arg Tyr Pro Leu Ala Lys Glu Pro Gly Arg
20 25 30
Ile Phe Gln Glu Glu Asn Asn Lys Asp Cys Asn Val Met Tyr Asn Arg
35 40 45
Asn Pro Gly Thr Phe Gly Pro Val Asn Thr Ala Gly Lys Val Ala Ser
50 55 60
Gly Ile Ile Gly Ser Val Met Arg Ser Phe Lys Arg Gln Lys Gln Ile
65 70 75 80
Asp Gln Ile Val Asp Ile Val Leu Ile Glu Val Tyr Ala Thr Leu Trp
85 90 95
Pro Ile Leu Glu Gly Met Trp Tyr Ala Met Met Asp Ala Val Glu Ile
100 105 110
Met Ile Gln Glu Ala Ile Thr Thr Ala Val Arg Ser Lys Ala Gln Ala
115 120 125
Glu Leu Asp Gly Val Arg Asn Ala Leu Ala Leu Tyr Gln Gln Ala Phe
130 135 140
Asp Asp Trp Glu Lys Asp Pro Asp Asn Leu Gln Leu Gln Glu Arg Ile
145 150 155 160
Arg Arg Gln Phe Thr Thr Thr Asn Thr Ile Ile Glu Phe Ala Met Pro
165 170 175
Ser Phe Ala Val Ser Gly Phe Glu Val Pro Leu Leu Val Val Tyr Ala
180 185 190
Gln Ala Ala Asn Leu His Leu Leu Phe Leu Arg Ala Ala Val Val Phe
195 200 205
Gly Glu Lys Trp Gly Met Ser Arg Glu Glu Ile Glu Asp Tyr Tyr Ser
210 215 220
Gly Asn Phe Gly Leu Thr Gly Arg Thr Lys Leu Tyr Thr Asn His Cys
225 230 235 240
Ile Asn Trp Tyr Asp Lys Gly Ile Glu Lys Ala Lys Val Leu Asn Pro
245 250 255
Ser Ala Thr Ile Leu Glu Gln Trp Asn Leu Phe Asn Asp Phe Arg Arg
260 265 270
Glu Met Thr Ile Met Val Leu Asp Ile Val Ala Leu Trp Pro Met Tyr
275 280 285
Asp Val Lys Leu Tyr Pro Glu Gly Ile Thr Thr Glu Leu Thr Arg Thr
290 295 300
Ile Tyr Thr Pro Ser Met Gly Ile Leu Glu Asp Ser Pro Met Met Ser
305 310 315 320
Met Val Arg Glu Asp His Gly Asn Leu Glu Ala Met Ala Ala Gln Glu
325 330 335
Leu Leu Ser Thr Ser Pro Pro Ser Leu Phe Thr Trp Leu Glu Lys Gln
340 345 350
Ile Val Tyr Arg Tyr Lys Asn Ala Ile Tyr Gly Tyr Gln Asn Phe Leu
355 360 365
Arg Thr Thr Phe Gly Glu Glu Phe Ser Ser Gly Ile Ile Gly Ala Thr
370 375 380
Asp Ser Pro Tyr Thr Ser Asn Pro Lys Ser Ile Pro Ile Gly Ser Glu
385 390 395 400
Leu Tyr Asp Val Tyr Lys Val Val Thr Ser Tyr Leu Glu Val Asp Pro
405 410 415
Tyr Pro Asn Lys Asp Ser Pro Ile His Arg Leu Val Tyr Tyr Arg Leu
420 425 430
Lys Asp Glu Gln Glu Ser Ile Ile Arg Thr Asp Thr Ser Glu Gly Pro
435 440 445
Ile Asp Gln Val Ser Glu Ile Thr Tyr Asp Glu Glu Phe Pro Glu Asn
450 455 460
Arg His Arg Leu Ala Tyr Met Ser Gly Leu Gln Ser Phe Phe Ser Leu
465 470 475 480
Ala Trp Thr Thr Glu Gly Leu Phe Asn Ser Tyr Trp Ala Pro Tyr Asn
485 490 495
Ile Thr Leu Gly Trp Thr Tyr Ala Asp Val Asp Arg Thr Asn Thr Ile
500 505 510
Ala Pro Asp Val Ile Thr Gln Ile Pro Ala Val Lys Gly Tyr Lys Ile
515 520 525
Ser Gly Asn Pro Tyr Leu Glu Gly Ala Glu Gly Ile Gly Ala Val Pro
530 535 540
Gly Pro Gly Phe Thr Gly Gly Asn Leu Val Gly Leu Ala Ala Gly Ala
545 550 555 560
Lys Leu Tyr Met Lys Val Thr Asn Pro Val Ser Asn Leu Val Gly Tyr
565 570 575
His Ile Arg Ile Arg Tyr Ala Asn Asn Gln Pro Thr Val Leu Ser Ile
580 585 590
Ser Tyr Gln Gly Leu Glu Thr Ser Ser Gly Arg Phe Asp Ile Pro Val
595 600 605
Thr Tyr Ser Gly Asp Tyr Thr Ala Lys Leu Thr Tyr Glu Ala Phe His
610 615 620
Tyr Lys Glu Thr Ile Val Ile Ser Ser Pro Ile Gly Glu Glu Val Ala
625 630 635 640
Glu Ile Met Leu His Asn Glu Gly Arg Ser His Leu Leu Ile Asp Lys
645 650 655
Ile Glu Phe Ile Pro Ile
660
<210> 101
<211> 328
<212> PRT
<213> 芽孢杆菌属的种
<400> 101
Met Arg Lys Tyr Lys Lys Leu Ala Val Ala Met Pro Leu Ala Gly Met
1 5 10 15
Ile Met Ser Gly Val Gly Val Leu Gly Gly Pro Ala Ser Thr Phe Ala
20 25 30
Asp Thr Met Pro Gln Ala Lys Val Ala Pro His Val Thr Asn Phe Ala
35 40 45
Ala Ala Asp His Tyr Gln Gln Phe Met Asp Phe Met Ser Ser Lys Gly
50 55 60
Met Glu Arg Tyr Ala Asn Asn Phe Glu Ile Val Ala Met Pro Asp Ser
65 70 75 80
Gly Pro Lys Asn Ile Arg Ile Thr Glu Ala Lys Pro Ile Asn Ala Pro
85 90 95
Glu Asn Thr Thr Gln Val Met Ser Tyr His Asn Ser Thr Gly Val Gln
100 105 110
Gln Ser Leu Lys Thr Pro Ser Lys Lys Ile Thr His Lys Asn Thr Ile
115 120 125
Ala Ile Lys Asn Gly Gly Ser Ile Lys Thr Thr Ile Glu Ser Lys Thr
130 135 140
Thr Phe Gly Val Gln Val Pro Phe Phe Ala Lys Gly Glu Gln Glu Leu
145 150 155 160
Lys Val Gly Leu Glu Val Thr Tyr Asn Arg Glu Ser Thr Asp Thr Ala
165 170 175
Glu Asn Ile Gln Glu Phe Thr Val Pro Glu Gln Thr Leu Leu Ala Gln
180 185 190
Pro Gly Gly Thr Thr Arg Leu Ile Tyr Thr Val Lys Ser Thr Ala Phe
195 200 205
Ser Gly Thr Tyr Thr Asn Lys Ala Ile Phe Lys Gln Lys Gln Asp Gly
210 215 220
Ile Ser Lys Lys Phe Glu Asn Gly Lys Glu Ile Glu Gly Lys Glu Leu
225 230 235 240
Glu Ser Trp Asn Glu Thr Gln Gly Tyr Arg Phe Ile Lys Gln Ile Ile
245 250 255
Ala Ser Gln Pro Gln Leu Ala Asp Ile Phe Glu Met Asp Asp Ala Asn
260 265 270
Glu Lys Ala Tyr Leu Lys Glu Ala Val Phe Thr Phe Glu Gly Gln Ala
275 280 285
Gly His Gln Ser Thr Ala Glu Ala Val Phe Ile Pro Asp Asp Asn Lys
290 295 300
Lys Ser Glu Val Lys Met Pro Ile Ala Glu Tyr Asn Lys Arg Val Ala
305 310 315 320
Asn Gly Ile Ser Leu Tyr Ala Lys
325
<210> 102
<211> 299
<212> PRT
<213> 芽孢杆菌属的种
<400> 102
Leu Gln Arg Lys Ser Tyr Leu Leu Gly Gly Glu Thr Met Ile His Asp
1 5 10 15
Leu Lys Glu Asp Ile Leu Asn Phe Thr Lys Trp Tyr Ala Glu Lys His
20 25 30
Tyr Asn Ala Asn Pro Asp Thr Phe His Asp Glu Lys Val Phe Asn Pro
35 40 45
Ser Val Ser Asn Thr Glu Val Val Pro Asp Lys Ile Glu Phe Asp Thr
50 55 60
Thr Pro Lys Leu Thr Ala Arg Ala Thr Asp Ile Leu Ile Asn Asp Thr
65 70 75 80
Ser Val Ser Gln Ser Ile Thr Pro Lys Ile Ser Thr Lys Thr Val Glu
85 90 95
Thr Thr Ser Ile Thr Thr Thr Glu Gly Tyr Lys Ile Gly Ala Gly Ile
100 105 110
Lys Tyr Thr Ser Thr Met Lys Leu Lys Leu Phe Leu Ile Gly Glu Ile
115 120 125
Ser Asn Ser Ile Glu Ile Ala Val Thr Ser Glu Tyr Asn His Ser Thr
130 135 140
Ser Glu Thr Gln Thr Asn Thr Thr Glu Lys Ile Trp Glu Tyr Thr Arg
145 150 155 160
Pro Val Ile Val Pro Pro Arg Thr Lys Val Thr Ala Thr Leu Glu Val
165 170 175
Tyr Ala Gly Pro Val Lys Val Pro Val Thr Leu Arg Ser Thr Ile Thr
180 185 190
Gly Met Gly Ile Ala Ser Thr Gly Tyr Gly Tyr Ile Leu Gly Ser Val
195 200 205
Ala Tyr Lys Asp Arg Asp Gly Lys Asn Trp Val Asp Ser Trp Arg Ser
210 215 220
Arg Ser Leu Tyr Asp Phe Lys Ser Thr Trp Pro Gly Phe Lys Pro Val
225 230 235 240
Tyr Val Gly Thr Asp Gly Glu Tyr Gly Val Lys Val Gln Gly Thr Ala
245 250 255
Gln Val Glu Leu Glu Leu Gly Leu Tyr Gly Val Val Thr Tyr Gln Glu
260 265 270
Glu Pro Leu Leu Gly Asn Asp Ser Asn Lys Ser Gln Thr Tyr Arg Glu
275 280 285
Ile Met Ser Arg Gln Gly Leu Tyr Thr Arg Ile
290 295
<210> 103
<211> 337
<212> PRT
<213> 赖氨酸芽孢杆菌属的种
<400> 103
Leu Glu Gly Glu Ser Asp Met Arg Ser Lys Thr Leu Arg Asn Ala Leu
1 5 10 15
Ile Ile Gly Met Val Phe Ile Thr Leu Gly Gly Thr Thr Thr Ile Lys
20 25 30
Pro Ser Gln Thr Tyr Ala Glu Ser Asn Ser Met Gln Glu Lys Thr Ile
35 40 45
Gly Ile Thr Asn Val Asn Gln Val Leu Glu Lys Ile Gly Asn Tyr Tyr
50 55 60
Tyr Gln Arg Asn Leu Ser Leu Thr Trp Tyr Glu Ala Pro Asn Ser Ile
65 70 75 80
Gly Val Lys His Asn Phe Gln Leu Arg Lys Asn Ser Asp Ser Ile Ser
85 90 95
Ser Glu Ile Asp Leu Ser Ile Ser Gly Glu Glu Ile Glu Ser Leu Tyr
100 105 110
Tyr Asp Ser Thr Ile Pro Lys Phe Leu Gly Glu Asn Val Phe Glu Asn
115 120 125
Asn Thr Asp Gln Glu Gln Ser Tyr Asn Thr Ser Lys Phe Ser Glu Thr
130 135 140
Tyr Thr Glu Ser Thr Ser Thr Ser Val Ser Lys Gly Phe Lys Ile Asn
145 150 155 160
Val Gly Arg Asp Phe Thr Ile Pro Leu Ile Leu Asn Glu Gly Gly Lys
165 170 175
Ile Asn Leu Glu Tyr Asn Ser Gly Ser Thr Asn Thr Asn Thr Leu Ser
180 185 190
Lys Thr Tyr Thr Leu Glu Val Pro Ser Gln Pro Val Lys Val Pro Pro
195 200 205
Asn Lys Ile Tyr Lys Ser Val Val Glu Tyr Ser Gln Arg Thr Tyr Lys
210 215 220
Gly Thr Val Lys Phe Tyr Gly Arg Asn Leu His Asn Pro His Pro Ile
225 230 235 240
Asn Thr Ile Lys Thr Thr Gly Ser Tyr Thr Gly Trp Met Gly Met Gln
245 250 255
Glu Ile Lys Gln Phe Thr Phe Asn Asp Pro Leu Tyr Lys His Tyr Asp
260 265 270
Gly Leu Ser Asp Ser Gln Lys Lys Glu Val Glu Asn Asp Gly Val Val
275 280 285
Ile Ile Pro Phe Leu Gly Ala Thr Tyr Val Val Val Glu Gly Lys Gly
290 295 300
Ser Phe Glu Gly Val Tyr Gly Ala Lys Leu Asn Val Lys Thr Tyr Asp
305 310 315 320
Ile Thr Asp Lys Asn Asn Ile Lys Leu Val Asp Ser Arg Ser Ile Asp
325 330 335
Leu
<210> 104
<211> 441
<212> PRT
<213> 芽孢杆菌属的种
<400> 104
Leu Tyr Ala His Lys Gln Pro Phe Phe Phe Ser Phe Leu Asn Val Gln
1 5 10 15
Ser Ser Ala Gly Asp Tyr His Tyr Ser Glu Phe Gln Arg Tyr Ser Ala
20 25 30
Gln Tyr Ala Asp His Cys Glu Leu Val Val Asn Asn Gln Leu Asn Lys
35 40 45
Ile Gln Asn Thr Thr Gly Lys Thr Trp Lys Asp Tyr Asn Glu Tyr Arg
50 55 60
Arg Lys Met Ile Leu Ser Val Phe Asp Ile Val Ala Glu Phe Ser Thr
65 70 75 80
Phe Asp Pro Ile Leu Tyr Lys Gly Ala Ile Asn Arg Glu Ile Leu Thr
85 90 95
Arg Lys Ile Tyr Thr Asp Pro Val Asn Phe Thr Pro Gly Ser Ser Ile
100 105 110
Ala Asn Asp Glu Asn Lys Tyr Thr Val Pro Pro Ser Arg Val Asn Gln
115 120 125
Leu Val Ala Ser Thr Leu Phe Thr Asn Val Ala Ser Ala Gln Tyr Ala
130 135 140
Gly Phe Ile Gly Asn Ile Asn Arg Tyr Leu Ser Leu Ser Arg Gly Glu
145 150 155 160
Pro Phe Asp Gly Pro Leu Ile Gly Asn Gln Val Tyr Glu Lys Val Leu
165 170 175
Ala Gly Val Pro Thr Asn Gln Ser Ile Tyr Glu Val Gly Val Glu Gly
180 185 190
Ile Gln Asn Asp Tyr Pro Arg Asn Ile Gly Leu Arg Trp Gly Ser Leu
195 200 205
Thr Ala Phe Gln Asn Phe Tyr Ala Gly Gly Thr Thr Asn Leu Gly Ser
210 215 220
Leu Thr Arg Val Ser Val Pro Pro Lys Asn Asn Asp Pro Ile Asn Ile
225 230 235 240
Thr Asn Phe Thr His Gln Leu Ser Asp Ile Ile Leu Pro Gly Asn Ser
245 250 255
Gly Ser Ser Phe Ala Trp Thr His Val Glu Val Asn Pro Thr Gly Asn
260 265 270
Tyr Leu Ser Thr Asn Gln Ile Asn Leu Ile Ala Gly Thr Lys Ala Ser
275 280 285
Glu Ser Pro Gln Ile Lys Lys Gly Pro Arg Phe Ile Gly Gly Asp Leu
290 295 300
Leu Thr Ala Asp Trp Arg Thr Gly Asn Ile Ile Ser Pro Ser Tyr Gln
305 310 315 320
Leu Ser Pro Lys Phe Pro Gly Thr Arg Thr Ser Phe Arg Val Arg Val
325 330 335
Arg Tyr Gly Ala Asn Ser Ser Gly Ser Met Gln Phe Thr Phe Gly Ala
340 345 350
Thr Thr Ser Ser Gln Leu Ala Tyr Thr Ala Thr Gly Tyr Ser Pro Thr
355 360 365
Asn Thr Lys Tyr Asn Gln Phe Arg Thr Ile Glu Phe Phe Thr Pro Leu
370 375 380
Glu Ile Thr Thr Glu Gly Lys Leu Ile Leu Val Leu Tyr Asn Thr Asn
385 390 395 400
Ser Ala Pro Thr Phe Val Ile Asp Arg Leu Glu Leu Ile Pro Met Ile
405 410 415
Gly Leu Pro Thr Glu Tyr Asn Glu Pro Gln Lys Leu Glu Thr Ala Lys
420 425 430
Lys Ala Val Thr Asp Leu Phe Thr Asn
435 440
<210> 105
<211> 415
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 105
Leu Tyr Ala His Lys Gln Pro Phe Phe Phe Ser Phe Leu Asn Val Gln
1 5 10 15
Ser Ser Ala Gly Asp Tyr His Tyr Ser Glu Phe Gln Arg Tyr Ser Ala
20 25 30
Gln Tyr Ala Asp His Cys Glu Leu Val Val Asn Asn Gln Leu Asn Lys
35 40 45
Ile Gln Asn Thr Thr Gly Lys Thr Trp Lys Asp Tyr Asn Glu Tyr Arg
50 55 60
Arg Lys Met Ile Leu Ser Val Phe Asp Ile Val Ala Glu Phe Ser Thr
65 70 75 80
Phe Asp Pro Ile Leu Tyr Lys Gly Ala Ile Asn Arg Glu Ile Leu Thr
85 90 95
Arg Lys Ile Tyr Thr Asp Pro Val Asn Phe Thr Pro Gly Ser Ser Ile
100 105 110
Ala Asn Asp Glu Asn Lys Tyr Thr Val Pro Pro Ser Arg Val Asn Gln
115 120 125
Leu Val Ala Ser Thr Leu Phe Thr Asn Val Ala Ser Ala Gln Tyr Ala
130 135 140
Gly Phe Ile Gly Asn Ile Asn Arg Tyr Leu Ser Leu Ser Arg Gly Glu
145 150 155 160
Pro Phe Asp Gly Pro Leu Ile Gly Asn Gln Val Tyr Glu Lys Val Leu
165 170 175
Ala Gly Val Pro Thr Asn Gln Ser Ile Tyr Glu Val Gly Val Glu Gly
180 185 190
Ile Gln Asn Asp Tyr Pro Arg Asn Ile Gly Leu Arg Trp Gly Ser Leu
195 200 205
Thr Ala Phe Gln Asn Phe Tyr Ala Gly Gly Thr Thr Asn Leu Gly Ser
210 215 220
Leu Thr Arg Val Ser Val Pro Pro Lys Asn Asn Asp Pro Ile Asn Ile
225 230 235 240
Thr Asn Phe Thr His Gln Leu Ser Asp Ile Ile Leu Pro Gly Asn Ser
245 250 255
Gly Ser Ser Phe Ala Trp Thr His Val Glu Val Asn Pro Thr Gly Asn
260 265 270
Tyr Leu Ser Thr Asn Gln Ile Asn Leu Ile Ala Gly Thr Lys Ala Ser
275 280 285
Glu Ser Pro Gln Ile Lys Lys Gly Pro Arg Phe Ile Gly Gly Asp Leu
290 295 300
Leu Thr Ala Asp Trp Arg Thr Gly Asn Ile Ile Ser Pro Ser Tyr Gln
305 310 315 320
Leu Ser Pro Lys Phe Pro Gly Thr Arg Thr Ser Phe Arg Val Arg Val
325 330 335
Arg Tyr Gly Ala Asn Ser Ser Gly Ser Met Gln Phe Thr Phe Gly Ala
340 345 350
Thr Thr Ser Ser Gln Leu Ala Tyr Thr Ala Thr Gly Tyr Ser Pro Thr
355 360 365
Asn Thr Lys Tyr Asn Gln Phe Arg Thr Ile Glu Phe Phe Thr Pro Leu
370 375 380
Glu Ile Thr Thr Glu Gly Lys Leu Ile Leu Val Leu Tyr Asn Thr Asn
385 390 395 400
Ser Ala Pro Thr Phe Val Ile Asp Arg Leu Glu Leu Ile Pro Met
405 410 415
<210> 106
<211> 565
<212> PRT
<213> 芽孢杆菌属的种
<400> 106
Val Asn His Met Tyr Ile Asn Thr Met Lys Asn Thr Leu Lys Ile Glu
1 5 10 15
Thr Thr Asp Tyr Glu Ile Asp Gln Ala Ala Ile Ser Ile Glu Cys Val
20 25 30
Ser Asn Glu Gln Asn Ser Gln Glu Lys Met Ile Leu Trp Asp Glu Ile
35 40 45
Lys Gln Ala Lys Gln Leu Ser Gln Ser Arg Asn Leu Leu Tyr Asn Gly
50 55 60
Asp Phe Glu Asp Ala Ser Asn Gly Trp Lys Thr Ser Tyr Thr Ile Glu
65 70 75 80
Ile Arg Lys Asn Ser Pro Ile Phe Lys Gly Gln Tyr Leu His Met Phe
85 90 95
Gly Ala Arg Asp Val Leu Gly Glu Val Phe Pro Thr Tyr Val Tyr Gln
100 105 110
Lys Ile Asp Glu Ser Lys Leu Lys Pro Tyr Thr Arg Tyr Arg Val Arg
115 120 125
Gly Phe Val Gly Ser Ser Lys Asp Leu Lys Leu Val Val Thr Arg Tyr
130 135 140
Gly Lys Glu Ile Asp Ala Ile Met Asp Val Pro Asp Asp Leu Ala Tyr
145 150 155 160
Met Gln Pro Asn Pro Ser Cys Gly Asp Tyr Arg Cys Asp Ser Ser Ser
165 170 175
Gln Ser Met Met Ser Gln Gly Tyr Pro Thr Pro Val Thr Asp Gly Tyr
180 185 190
Ala Ser Val Met Tyr Ala Cys Pro Ser Asp Arg Val Lys Lys His Val
195 200 205
Lys Cys His Asp Arg His Pro Phe Asp Phe His Ile Asp Thr Gly Lys
210 215 220
Leu Asp Thr Asn Thr Asn Leu Gly Met Leu Val Leu Phe Lys Ile Ser
225 230 235 240
His Pro Asn Gly Tyr Ala Thr Leu Gly Asn Leu Glu Val Ile Glu Glu
245 250 255
Gly Pro Leu Thr Asp Glu Ala Leu Glu His Val Lys Gln Lys Glu Lys
260 265 270
Lys Trp Asn Arg His Ile Glu Lys Ala Arg Met Glu Thr Gln Gln Ala
275 280 285
Tyr Asp Pro Ala Lys Gln Ala Val Asp Ala Leu Phe Thr Ser Val Gln
290 295 300
Glu Leu His Tyr His Thr Thr Leu Asn His Ile Lys Asn Ala Asp Gln
305 310 315 320
Leu Val Gln Ser Ile Pro Tyr Val Asn His Ala Trp Leu Pro Asp Ala
325 330 335
Pro Ser Met Asn Tyr Asp Leu Tyr Gln Gly Leu Asn Ala Arg Ile Met
340 345 350
Gln Ala Tyr Asn Leu Tyr Asp Ala Arg Asn Val Ile Thr Asn Gly Asp
355 360 365
Phe Thr Gln Gly Leu His Gly Trp His Thr Thr Gly Asn Ala Ala Val
370 375 380
Gln Gln Met Asp Gly Ala Ser Val Leu Val Leu Ser Asn Trp Ser Ala
385 390 395 400
Gly Val Ser Gln Asn Leu His Ala Gln Asp His His Gly Tyr Val Leu
405 410 415
Arg Val Ile Ala Lys Lys Glu Gly Thr Gly Lys Gly Tyr Val Thr Met
420 425 430
Met Asp Cys Asn Gly Lys Gln Glu Thr Leu Thr Phe Thr Ser Cys Glu
435 440 445
Glu Gly Tyr Met Thr Lys Thr Val Glu Val Phe Pro Glu Ser Asp Arg
450 455 460
Val Arg Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Tyr Ile Asp Ser
465 470 475 480
Ile Glu Leu Leu Cys Met Lys Gly Tyr Thr Ser Asn Tyr Asn Gln Asn
485 490 495
Thr Gly Asn Met Tyr Glu Gln Ser Tyr Asn Gly Asn Tyr Asn Gln Asn
500 505 510
Thr Ser Asp Val Tyr His Gln Gly Tyr Thr Asn Asn Tyr Asn Lys Asp
515 520 525
Ser Ser Ser Met Tyr Asn Gln Asn Tyr Thr Asn Asn Asp Asp Gln His
530 535 540
Ser Gly Cys Thr Cys Asn Gln Gly His Asn Pro Gly Cys Thr Cys Asn
545 550 555 560
Gln Arg Tyr Asn Arg
565
<210> 107
<211> 316
<212> PRT
<213> 链霉菌属的种
<400> 107
Val Ala Gly Gly Gly Arg Gly Ala Arg Pro Gly Gly Gly Val His Arg
1 5 10 15
His Arg Ala Arg Pro His Arg Glu Gln His Pro Gly Pro Pro Arg Arg
20 25 30
Gly Glu Glu Gly Pro Glu Ala Ala Gln Gly Ala Ala Gly Arg Glu Ala
35 40 45
Gly Glu Val Arg Glu Ala Gly Arg Arg Leu Pro Asp Arg Arg Gln Ala
50 55 60
Arg Gly Val Pro Gln Gly Arg Pro Gly Leu Arg Pro Gln Arg Glu Ala
65 70 75 80
Ala Pro Arg Pro Arg Leu Asn Arg Pro His Gly Gly Arg Arg Ala Pro
85 90 95
Gly Arg Gly Arg Leu Gly Ile Leu Thr Pro Pro Asp His Arg Ile Asp
100 105 110
His Arg Ile Asp His Arg Asp His Pro Gly Gly Ala Leu Ala Ala Ser
115 120 125
Phe Arg Thr Val Val Glu Val Ala Gln Asp His Leu Ala Gln Ala Arg
130 135 140
Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro Ala Thr Val Asn
145 150 155 160
Phe Asp Phe Glu Ala Ile His Arg Ala Ala Ser Ala Val Pro Asp Ser
165 170 175
Asp Val Val Lys Met Val Arg Gly Trp Gly Leu Gln Glu His Ala Pro
180 185 190
Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val Arg Gln Ala Leu
195 200 205
Pro Pro Glu Cys Ala Glu Ala Gly Phe Trp Ala Thr Val Glu Arg Glu
210 215 220
Leu Val Gln Ala Phe Thr Gly Leu Ala Ala Gln Glu Gly Arg Pro Gly
225 230 235 240
Leu Ser Phe Tyr Glu Glu Ser Gly Asp Arg Thr Arg Phe Tyr Arg Asp
245 250 255
Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Asp His Met Tyr Ala
260 265 270
Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro Lys Ala Glu Thr
275 280 285
Leu Ala Leu Asp Leu Thr Asp Val Val Ser Phe Ala Val Arg Leu Asn
290 295 300
Ala Ile Val Val Arg Gln Ala Leu Ala Val Ser Val
305 310 315
<210> 108
<211> 336
<212> PRT
<213> 芽孢杆菌属的种
<400> 108
Met Leu Ile Val Cys Tyr Leu Arg Ser Tyr Ile Phe Asn Gly Gly Asn
1 5 10 15
Lys Met Thr Asn Thr Gln Met Asp Asn Lys Tyr Leu Phe Arg Pro Ser
20 25 30
Tyr Glu Ala Lys Pro Asp Gly Thr Thr Val Asn Pro Tyr Tyr Ile Ile
35 40 45
Ile Asn Asp Leu Lys Arg Trp Pro Cys Tyr Val Thr Asp Asn Arg Asn
50 55 60
Phe Ile Ser Ser Ser Glu Glu Leu Gln Thr Arg Val Ala Pro Ser Tyr
65 70 75 80
Asp Pro Thr Gly Lys Tyr Asp Gly Thr Pro Ser Ala Asp Phe Asn Arg
85 90 95
Val Tyr Arg Asp Tyr Ala Leu Asp Pro Ala Phe Arg Pro Leu Tyr Ala
100 105 110
Ile Asn Ala Gln Ile Leu Pro Lys Tyr Asn Tyr Trp His Gln Gly Asn
115 120 125
Trp Gln Tyr Asn Glu Leu Gly Arg Val Ser Gln Leu Gly Gln Asp Ile
130 135 140
Gln Asn Phe Ala Asn Leu Ile Cys Val Lys Tyr Ile Asn Glu Leu Asn
145 150 155 160
Gln Ile Ile Asp Asp Thr Gln Asn Asp Lys Leu Ser Gln Lys Asp Ile
165 170 175
Asp Thr Phe Lys Asn Ile Cys Ser Asp Leu Gln Ser Gln Ile Gly Pro
180 185 190
Cys Gln Arg Arg Ser Arg Asn Ile Tyr Asn Glu Tyr Gln Asn Ala Ile
195 200 205
Asn Ile Leu Leu Ser Gln Ser His His Asp Ala Ala Asn Tyr Ala Phe
210 215 220
Thr Ile Val Asp Asn Ala Tyr Ser Thr Ala Lys Ser Gly Asp Gln Asn
225 230 235 240
Ala Leu Gly Gly Ile Arg Ser Tyr Ala Ser Ser Val Leu Asn Tyr Ala
245 250 255
Asn Ala Tyr Asp Gln Gly Val Ile Thr Ala Ser Met Asn Leu Gln Gly
260 265 270
Met Trp Met Phe Phe Gly Asp Asn Leu Gln Glu Leu Ile Ser Asn Leu
275 280 285
Lys Asp Pro Ile Val Asp His Asn Ala Leu Ile Ala Ser Leu Gln Leu
290 295 300
Glu Ile Ala Ile Glu Glu Trp Gln Asn Val Ser Thr Asn Ala Ala Glu
305 310 315 320
Phe Ile Lys Glu Trp Ala Gly Tyr Gly Ala Ser Leu Gly Tyr Asp Cys
325 330 335
<210> 109
<211> 307
<212> PRT
<213> 芽孢杆菌属的种
<400> 109
Leu Ser Ile Tyr Asn Tyr Arg Ile Thr Ser Trp Phe Gly Asp Arg Ser
1 5 10 15
Ile Leu Pro Ser Asn Glu Thr Leu Gln Gln Gln Ser Trp Thr Gly Glu
20 25 30
Ala Lys Gln Lys Trp Asn Leu Arg Asp Leu Gly Asp Tyr Ser Phe Glu
35 40 45
Ile Val Asn Gln Ala Asn Gly Lys Val Pro Thr Tyr Gln Trp Ala Gly
50 55 60
Ser Leu Val Asp Asn Val Glu Tyr Leu Lys Leu Asp Tyr Ser Asn Pro
65 70 75 80
Ser Asp Pro Asp Lys Val Phe Gly Ile Ile Asn Arg Ser Phe Leu Phe
85 90 95
Asp Gly Thr Asn Tyr Ala Ile His Ala Glu Asp Leu Val Lys Ile Gln
100 105 110
Lys Leu Pro Asp Val Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr Gly
115 120 125
Gly Val Asp Gln Gln Leu Pro Gln Val Ser Asp Ser Val Val Val Gly
130 135 140
Ala Ser Leu Ile Pro Cys Ile Met Val Asn Asp Asn Gln Ala Ser Asp
145 150 155 160
Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu
165 170 175
Tyr Trp Asp Lys Thr Tyr Ser Ala Val Ile Pro Ala Gly Val Thr His
180 185 190
Lys Tyr Ser Phe Thr Thr Gly Met Ser Ser Val Asp Gln Gln Lys Met
195 200 205
Thr Asp Thr Leu Ser Met Ser Ile Gly Ala Asp Phe Gly Leu Ser Phe
210 215 220
Gly Ala Ala Ser Ala Ser Leu Lys Thr Glu Ile Ser Arg Thr Ile Gln
225 230 235 240
Thr Glu Ile Ser Ser Thr Asn Thr Asn Ala Ser Glu Glu Asn Val Thr
245 250 255
Arg Asp Val Gln Ser Glu Pro Gly Lys Thr Thr Gly Phe Thr Glu Tyr
260 265 270
Gln Leu Ala Thr Arg Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val
275 280 285
Ser Asp Pro Trp Val Val Lys Asn Asn Lys Ile Thr Ile Thr Arg Lys
290 295 300
Asn Ala Gln
305
<210> 110
<211> 328
<212> PRT
<213> 赖氨酸芽孢杆菌属的种
<400> 110
Met Arg Lys Asn Lys Lys Ile Val Gln Lys Thr Leu Thr Ile Ala Ala
1 5 10 15
Ile Ala Ser Leu Gly Thr Thr Leu Ala Ile Ser Ser Pro Ser Leu Ala
20 25 30
Ser Ala Asp Gln Ile Asn Thr Ser Glu Asn Asn Leu Gln Arg Glu Val
35 40 45
Lys Ala His Thr Ile Leu Asp Trp Lys Asp Pro Ile Phe Asn Ala Thr
50 55 60
Glu Ile Val Gly Lys Asn Leu Leu Val Pro Gly Ile Tyr Leu Phe Thr
65 70 75 80
Asn Ser Thr Tyr His His Met Gly Tyr Tyr Gly Val Lys Tyr Glu Gln
85 90 95
Phe Ser Val Glu Ala Asp Gly Ser Pro Ser His Thr Asn Ser Thr Ser
100 105 110
Ile Phe Val Gly Lys Thr Thr Leu Thr Asn Ile Thr Asp Gln Glu Gln
115 120 125
Thr Leu Ser Thr Asn Ser Phe Ser Lys Met Ile Ser Asn Ser Val Ser
130 135 140
Asn Ser Thr Thr His Gly Phe Lys Phe Gly Thr Lys Ala Ser Ala Lys
145 150 155 160
Phe Gln Ile Pro Phe Val Gly Glu Thr Gly Ile Glu Leu Ser Ala Glu
165 170 175
Tyr Asn Phe Ser Asp Thr Ser Ser Thr Thr Asn Thr Glu Ser Tyr Thr
180 185 190
Tyr Ile Ala Ser Pro Gln Asn Ile Lys Val Pro Ala His Ser Thr Val
195 200 205
Glu Val Leu Val Asn Leu Asn Thr Val Lys Ala Lys Gly Asn Val Lys
210 215 220
Leu Leu Thr Lys Val Ser Gly Arg Asp Gln Gly Val Phe Val Phe Asp
225 230 235 240
Pro Gln Thr Gly Asp Ile Ser Lys Ser Tyr Tyr Tyr Asp Leu His Phe
245 250 255
Asn Gln Ile Val Glu Lys Ala Ser Lys Phe Glu Lys Leu His Asn Ile
260 265 270
Ser Ser Asn Ser Asp Gly Asn Thr Val Asn Ile Ile Gly Ser Gly Lys
275 280 285
Tyr Glu Ala Glu Tyr Gly Thr Glu Phe Ser Val Thr Val Arg Pro Val
290 295 300
Asp Asn Lys Gly Arg Ser Ile Asn Gly Gly Tyr Thr Phe Asn Val Lys
305 310 315 320
Pro Glu Lys Ile Ile Lys Val Glu
325
<210> 111
<211> 338
<212> PRT
<213> 类芽孢杆菌属的种(Paenibacillus sp.)
<400> 111
Met Arg Lys His Val Leu Lys His Gly Leu Val Ile Gly Leu Ser Ala
1 5 10 15
Leu Met Leu Gly Ser Ala Ser Ala Pro Pro Gln Val Tyr Ala Glu Ala
20 25 30
Ser Ile Ala Gln Glu Lys Ser Ile Gly Ile Val Asp Val Asn Glu Val
35 40 45
Leu Lys Lys Val Gly Thr Tyr Tyr Tyr Lys Asn His Leu Ala Asn Thr
50 55 60
Val Ala Asn Pro Glu Ser Asn Gly Phe Arg Tyr Thr Leu Arg Ala Asn
65 70 75 80
Pro Asp Ser Val Thr Pro Val Ile Asp Leu Ser Ile Glu Ala Arg Thr
85 90 95
Glu Asn Ile His Tyr Asp Ser Thr Ile Pro Ile Phe Ile Gly Glu Asn
100 105 110
Thr Phe Pro Asn Asn Leu Asp Glu Glu Gln Ser Tyr Asn Thr Leu Lys
115 120 125
Tyr Ser Lys Thr Tyr Thr Glu Ser Thr Ser Thr Ser Ile Ser Ser Gly
130 135 140
Phe Lys Val Gly Gly Ala Gly Asp Leu Gly Asn Lys Phe Ile Leu Pro
145 150 155 160
Leu Ile Leu Glu Gly Ala Gly Lys Ile Asn Leu Glu Tyr Asn Thr Gly
165 170 175
Ser Thr Glu Thr Asn Thr Thr Ser Glu Ser Tyr Thr Leu Glu Val Pro
180 185 190
Pro Gln Pro Val Lys Val Pro Pro His Lys Thr Tyr Lys Thr Val Val
195 200 205
Glu Phe Ser Gln Arg Thr Tyr Arg Gly Asp Val Val Phe Thr Gly Arg
210 215 220
Thr Glu Ser Thr Met Ala Asn Arg Ser Ile Ile Asn Ala Thr Gly Ile
225 230 235 240
Tyr Thr Gly Trp Met Gly Met Gln Asp Ser Lys Lys Phe Thr Tyr Asp
245 250 255
Asn Tyr Leu Gly Val His Tyr Ala Gly Leu Thr Ala Ser Gln Gln Gln
260 265 270
Glu Ile Lys Asn Ala Gly Val Gln Ile Thr Thr Ser Gly Tyr Gly Ala
275 280 285
Ala Ala Asn Arg Phe Ser Ser Ile Glu Val Thr Gly Lys Gly Ser Phe
290 295 300
Ser Gly Val Ile Gly Ala Glu Leu Ile Val Thr Thr Tyr Asp Val Thr
305 310 315 320
Asp Ala Lys Asn Ala Arg Leu Val Asn Gln Arg Thr Val Pro Ile Glu
325 330 335
Leu Asp
<210> 112
<211> 684
<212> PRT
<213> 芽孢杆菌属的种
<400> 112
Met Asp Ala Leu Ile Tyr Val Asp Tyr Ile Tyr Ile Asp Thr Ile Ser
1 5 10 15
Ile Ile Asp Ile Glu Asn Ile Tyr Val Lys Asn Gly Gly Ser Leu Met
20 25 30
Lys Arg Asn Lys Asp Cys Asn Asp Gly Ile Gln Lys Ser Asp Met Ser
35 40 45
Asp Arg Tyr Pro Tyr Ala Asn Asp Pro Asn Ala Ala Leu Gln Asn Met
50 55 60
Glu Tyr Lys Glu Trp Leu Ser Val Cys Gln Glu Gly Gly Gln Gln Val
65 70 75 80
Leu His Leu Glu Ser Val Gly Asn Glu Thr Val Asn Ala Val Ser Ala
85 90 95
Gly Val Ile Val Val Gly Thr Met Met Ser Ala Met Ser Ala Pro Leu
100 105 110
Ala Ile Pro Gly Gly Ile Leu Ile Ser Phe Gly Thr Leu Ala Ala Ala
115 120 125
Leu Trp Pro Asp Glu Glu Asn Pro Thr Lys Val Trp Ser Asp Phe Met
130 135 140
Lys His Gly Glu Thr Leu Phe Asp Lys Lys Ile Ser Glu Leu Val Lys
145 150 155 160
Lys Gln Ala Gln Ala His Phe Glu Gly Ile Lys Thr Leu Leu Val Asp
165 170 175
Tyr Glu Lys Ala Phe Asn Asp Trp Lys Glu Asn Lys Asn Arg Leu Thr
180 185 190
Ala Glu Lys Val Lys Arg Glu Phe Asn Asp Val Asp Arg Gln Ile Lys
195 200 205
Thr Val Ile Pro His Leu Lys Tyr Glu Gly Tyr Glu Ala Leu Leu Leu
210 215 220
Ser Cys Phe Ala Gln Val Ala Asn Leu His Leu Asn Leu Leu His Gln
225 230 235 240
Gly Ala Ala Phe Gly Asp Glu Trp Asp Lys Tyr Ser Ser Tyr Leu Ser
245 250 255
Thr Ser Glu Asp Cys Tyr Glu Asp Leu Met Ser Arg Ile Glu Thr Tyr
260 265 270
Ile Asn His Cys Thr Lys Thr Tyr Asn Asp Gly Leu Ile Glu Leu Lys
275 280 285
Asn Lys Pro Asn Ile Lys Trp Asn Ile Tyr Asn Thr Tyr Arg Arg Glu
290 295 300
Met Thr Leu Thr Ala Leu Asp Leu Val Ala Leu Phe Pro Asn Tyr Asp
305 310 315 320
Val Lys Lys Tyr Pro Ile Gly Thr Gln Thr Glu Leu Thr Arg Glu Val
325 330 335
Tyr Thr Val Leu Asn Ser Met Asp Asp Leu Val Asp Phe Glu Ala Ala
340 345 350
Glu Lys Ser Leu Ile Gln Asn Pro His Val Phe Arg Trp Leu Ala Gly
355 360 365
Phe Thr Phe Val Thr Lys Asn Ile Ser Ala Ile Asp Leu Lys Tyr Leu
370 375 380
Ser Gly Ile Arg Asn Trp Tyr Tyr Tyr Thr Asn Phe Asp Thr Leu Lys
385 390 395 400
Ala Asp Lys Ala Asn Ser Glu Asn Ser Gly Gly Gly Ser Gln Lys Phe
405 410 415
Phe Phe Gly Ala Gly Ser Ile Tyr Lys Leu Glu Ile Ile Arg Asn Met
420 425 430
Asp Asp His Trp Gly Gly Leu Pro Phe Ala Asn Glu Val Gln Val Phe
435 440 445
Thr Pro Ser Ile Thr Tyr Asp Asn Lys Gln Ser Gln Thr Asn Ser Val
450 455 460
Tyr Asn Lys Lys Gly Asp Ala Ser Ser Gly Ile Pro Ile Met Asp Leu
465 470 475 480
Leu Tyr Leu Pro Lys Ala Lys Asp Asp Ser Pro Phe Ser Gln Gln Asp
485 490 495
Ile Pro Ser Ala Asn Asn Tyr Ser His Arg Leu Ser Tyr Ile Ala Arg
500 505 510
Val Lys Lys Ile Arg Gly Thr Arg Asp Val Tyr Asn Tyr Ala Phe Ala
515 520 525
Trp Thr His Arg Ser Val Asn Arg His Asn Thr Ile Ala Lys Asp Val
530 535 540
Ile Thr Gln Ile Pro Ala Val Lys Ala Leu Glu Pro Asn Lys Asp Phe
545 550 555 560
Asp Val Ile Lys Gly Pro Gly His Thr Gly Gly Asp Leu Val Lys Leu
565 570 575
Asn Tyr Asn Glu Thr Ser Gly Leu Glu Ile Lys Cys Leu Thr Glu Asn
580 585 590
His Pro Lys Ile Asp Tyr Arg Ile Arg Ile Arg Tyr Ala Ala Asn Glu
595 600 605
Asp Thr Arg Val Glu Ile Ile Val Pro Glu Ser Asp Gly Phe Gly Ser
610 615 620
Ile Lys Pro Ala Lys Lys Thr Tyr Ser Asp Asp Asn Tyr Gln Lys Tyr
625 630 635 640
Asn Ser Phe Gln Tyr Leu Asp Thr Arg Ala Val Val Tyr Gly Glu Gly
645 650 655
Lys Gln Val Ala Leu Ser Ile Lys Asn Leu Glu Pro Asp Thr Phe Ile
660 665 670
Leu Ile Asp Lys Ile Glu Phe Ile Pro Trp Phe Gly
675 680
<210> 113
<211> 682
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 113
Met Asp Ala Leu Ile Tyr Val Asp Tyr Ile Tyr Ile Asp Thr Ile Ser
1 5 10 15
Ile Ile Asp Ile Glu Asn Ile Tyr Val Lys Asn Gly Gly Ser Leu Met
20 25 30
Lys Arg Asn Lys Asp Cys Asn Asp Gly Ile Gln Lys Ser Asp Met Ser
35 40 45
Asp Arg Tyr Pro Tyr Ala Asn Asp Pro Asn Ala Ala Leu Gln Asn Met
50 55 60
Glu Tyr Lys Glu Trp Leu Ser Val Cys Gln Glu Gly Gly Gln Gln Val
65 70 75 80
Leu His Leu Glu Ser Val Gly Asn Glu Thr Val Asn Ala Val Ser Ala
85 90 95
Gly Val Ile Val Val Gly Thr Met Met Ser Ala Met Ser Ala Pro Leu
100 105 110
Ala Ile Pro Gly Gly Ile Leu Ile Ser Phe Gly Thr Leu Ala Ala Ala
115 120 125
Leu Trp Pro Asp Glu Glu Asn Pro Thr Lys Val Trp Ser Asp Phe Met
130 135 140
Lys His Gly Glu Thr Leu Phe Asp Lys Lys Ile Ser Glu Leu Val Lys
145 150 155 160
Lys Gln Ala Gln Ala His Phe Glu Gly Ile Lys Thr Leu Leu Val Asp
165 170 175
Tyr Glu Lys Ala Phe Asn Asp Trp Lys Glu Asn Lys Asn Arg Leu Thr
180 185 190
Ala Glu Lys Val Lys Arg Glu Phe Asn Asp Val Asp Arg Gln Ile Lys
195 200 205
Thr Val Ile Pro His Leu Lys Tyr Glu Gly Tyr Glu Ala Leu Leu Leu
210 215 220
Ser Cys Phe Ala Gln Val Ala Asn Leu His Leu Asn Leu Leu His Gln
225 230 235 240
Gly Ala Ala Phe Gly Asp Glu Trp Asp Lys Tyr Ser Ser Tyr Leu Ser
245 250 255
Thr Ser Glu Asp Cys Tyr Glu Asp Leu Met Ser Arg Ile Glu Thr Tyr
260 265 270
Ile Asn His Cys Thr Lys Thr Tyr Asn Asp Gly Leu Ile Glu Leu Lys
275 280 285
Asn Lys Pro Asn Ile Lys Trp Asn Ile Tyr Asn Thr Tyr Arg Arg Glu
290 295 300
Met Thr Leu Thr Ala Leu Asp Leu Val Ala Leu Phe Pro Asn Tyr Asp
305 310 315 320
Val Lys Lys Tyr Pro Ile Gly Thr Gln Thr Glu Leu Thr Arg Glu Val
325 330 335
Tyr Thr Val Leu Asn Ser Met Asp Asp Leu Val Asp Phe Glu Ala Ala
340 345 350
Glu Lys Ser Leu Ile Gln Asn Pro His Val Phe Arg Trp Leu Ala Gly
355 360 365
Phe Thr Phe Val Thr Lys Asn Ile Ser Ala Ile Asp Leu Lys Tyr Leu
370 375 380
Ser Gly Ile Arg Asn Trp Tyr Tyr Tyr Thr Asn Phe Asp Thr Leu Lys
385 390 395 400
Ala Asp Lys Ala Asn Ser Glu Asn Ser Gly Gly Gly Ser Gln Lys Phe
405 410 415
Phe Phe Gly Ala Gly Ser Ile Tyr Lys Leu Glu Ile Ile Arg Asn Met
420 425 430
Asp Asp His Trp Gly Gly Leu Pro Phe Ala Asn Glu Val Gln Val Phe
435 440 445
Thr Pro Ser Ile Thr Tyr Asp Asn Lys Gln Ser Gln Thr Asn Ser Val
450 455 460
Tyr Asn Lys Lys Gly Asp Ala Ser Ser Gly Ile Pro Ile Met Asp Leu
465 470 475 480
Leu Tyr Leu Pro Lys Ala Lys Asp Asp Ser Pro Phe Ser Gln Gln Asp
485 490 495
Ile Pro Ser Ala Asn Asn Tyr Ser His Arg Leu Ser Tyr Ile Ala Arg
500 505 510
Val Lys Lys Ile Arg Gly Thr Arg Asp Val Tyr Asn Tyr Ala Phe Ala
515 520 525
Trp Thr His Arg Ser Val Asn Arg His Asn Thr Ile Ala Lys Asp Val
530 535 540
Ile Thr Gln Ile Pro Ala Val Lys Ala Leu Glu Pro Asn Lys Asp Phe
545 550 555 560
Asp Val Ile Lys Gly Pro Gly His Thr Gly Gly Asp Leu Val Lys Leu
565 570 575
Asn Tyr Asn Glu Thr Ser Gly Leu Glu Ile Lys Cys Leu Thr Glu Asn
580 585 590
His Pro Lys Ile Asp Tyr Arg Ile Arg Ile Arg Tyr Ala Ala Asn Glu
595 600 605
Asp Thr Arg Val Glu Ile Ile Val Pro Glu Ser Asp Gly Phe Gly Ser
610 615 620
Ile Lys Pro Ala Lys Lys Thr Tyr Ser Asp Asp Asn Tyr Gln Lys Tyr
625 630 635 640
Asn Ser Phe Gln Tyr Leu Asp Thr Arg Ala Val Val Tyr Gly Glu Gly
645 650 655
Lys Gln Val Ala Leu Ser Ile Lys Asn Leu Glu Pro Asp Thr Phe Ile
660 665 670
Leu Ile Asp Lys Ile Glu Phe Ile Pro Trp
675 680
<210> 114
<211> 311
<212> PRT
<213> 芽孢杆菌属的种
<400> 114
Met Val Ile Phe Asp Ile Asp Gln Tyr Leu Tyr Asn Leu Asp Leu Ala
1 5 10 15
Leu Thr His Thr Met Val Ala His Ser Thr Gly Asp Leu Glu His Cys
20 25 30
Lys Ala Asn Asn Tyr Tyr Tyr Asp Glu Val Tyr Tyr Asn Glu Val Asn
35 40 45
Ile Asn Pro Leu His Tyr Asn Asn Ser Arg Val Ile Leu Ser Asn Gln
50 55 60
Lys Leu Asp Tyr Asn Leu Pro Pro Leu Ser Ser Glu Asn Val Ser Pro
65 70 75 80
Leu Val Val Ser Gly Gly Thr Phe Lys Asn Asn Thr Asn Asp Val Gln
85 90 95
Thr Tyr Thr Ser Leu Glu Ile Thr Arg Lys Gln Gly Thr Ser Gln Lys
100 105 110
Ser Ser Ile Thr Tyr Gly Phe Lys Ala Gly Thr Lys Leu Ser Ala Ser
115 120 125
Tyr Lys Leu Lys Gly Gly Ile Ser Leu Ile Ala Gly Gly Glu Thr Ser
130 135 140
Phe Asn Phe Glu Phe Ser Phe Glu Ala Asn Phe Ser Lys Thr Asp Glu
145 150 155 160
Thr Val Glu Thr Asp Glu Thr Thr Val Arg Val Pro Pro Gln Thr Ile
165 170 175
Ile Val Lys Pro Gly Glu Thr Lys Arg Leu Glu Val Thr Leu Tyr Arg
180 185 190
Ile Val Ile Pro Asp Thr Lys Phe Asp Ile Asp Gly Asp Ile Ser Gly
195 200 205
Lys Ile Gln Tyr Gly Ser Gly Asn Pro Tyr Val Asp Ile Tyr Glu Thr
210 215 220
Phe Asp Tyr Val Gln Lys His Cys Pro Gly Thr Tyr Val Asn Arg Ser
225 230 235 240
Gly Asp Asp Leu Ile Arg Leu Asn Ser Ser Lys Lys Thr Leu Arg Phe
245 250 255
Lys Gly Phe Gly Lys Val Ala Phe Glu Tyr Glu Met Asn Met Ile Thr
260 265 270
Ile Asp Asn Lys Thr Gly Lys Val Val His Glu Glu Arg Val Pro Val
275 280 285
Asp Gly Tyr His Pro Ile Ser Tyr Lys Phe Gln Asp Gly Gln Lys Pro
290 295 300
Val Pro Met Gln Ile Phe Gly
305 310
<210> 115
<211> 296
<212> PRT
<213> 芽孢杆菌属的种
<400> 115
Met Lys Ile Asn Lys Leu Lys Glu Glu Pro Gln Asn Phe Tyr Thr Leu
1 5 10 15
Ile Ala Lys Glu Leu Lys Leu Val Ser Ser Cys Tyr Phe Ala Tyr Ile
20 25 30
Tyr Pro Tyr His His Val Thr Asp Asn Lys Thr Leu Ala Asn Leu Leu
35 40 45
Tyr Pro Pro Tyr Thr Asp Arg Gly Val Ile Asn Phe Asp Phe Phe Arg
50 55 60
Lys His Arg Gln Phe Val His Phe Phe His Ile Tyr Glu Met Asp Asp
65 70 75 80
Val Phe His Ser Leu Met Asn Ile His Ala Gly Ile Tyr Asn Ser Asp
85 90 95
Leu Trp Trp Lys Leu Leu Gly Asp Gln Lys Val Asn Leu Leu Ser Gln
100 105 110
Asp Ile Glu Ser Phe Ala Asp Ile Ile Cys Val Glu His Ile Asp Glu
115 120 125
Leu Asn Glu Ile Ile Lys Asn Ala Asp Asn Asn Lys Leu Ser Glu Lys
130 135 140
Asn Ile Asp Thr Leu Lys Lys Ile Tyr Asp Glu Leu Asn Lys Asn Ile
145 150 155 160
Gly Asn Tyr Asn Gln Lys Ala Glu Glu Ile Leu Asn Gln Cys Asn Gln
165 170 175
Leu Leu Gln Phe Ile Val Ser Tyr Ser Gly Lys Ile Asn Asp Thr Ile
180 185 190
Ser Lys Ile Lys Ser Ile Tyr Met Ile Ser Lys Pro Thr Asp Lys Asn
195 200 205
Tyr Phe Glu Asp Leu Leu Leu Phe Leu Lys Lys Glu Lys Ile Val Ile
210 215 220
Asp Arg Tyr Asn Pro Glu Ile Ile Thr Ile Leu Met Asn Leu Gln Ala
225 230 235 240
Ile Trp Arg Val Phe Ala Ser Ser Leu Gln Asp Leu Ile Gln Glu Trp
245 250 255
Glu Asn Pro Leu Ile Asp Asp Asn Thr Leu Val Phe Ser Leu Glu Leu
260 265 270
Lys Ala Ala Ile Ala Thr Tyr Glu Gln Ile Lys Gln Gly Ile Glu Thr
275 280 285
Phe Arg Ile Lys Trp Lys Asp Asp
290 295
<210> 116
<211> 326
<212> PRT
<213> 短芽孢杆菌属的种(Brevibacillus sp.)
<400> 116
Met Lys Lys Lys Met Asn Lys Asn Arg Leu Lys Lys Pro Met Val Ala
1 5 10 15
Ile Met Leu Ala Thr Ser Ile Gly Ile Pro Cys Ile Ser Ala Ser Gly
20 25 30
Ser Ala Leu Ala Thr Glu Asn Thr Pro Thr Ser Val Asn Glu Asn Val
35 40 45
Lys Val Gly Val Thr Asp Val Gln Ser Glu Leu Lys Lys Ile Gly Glu
50 55 60
Tyr His Tyr Thr Asn Asn Ile Ala Gly Thr Lys Ile Glu Ser Trp Pro
65 70 75 80
Thr Leu Thr Phe Gly Ser Arg Pro Asp Ser Val Glu Thr Lys Val Asn
85 90 95
Phe Ser Ile Thr Gly Thr Glu Asn Lys Leu Asn Phe Asp Ser Lys Ile
100 105 110
Val Glu Tyr Val Gly Glu Asn Ile Phe Glu Asn Thr Thr Glu Gln Ser
115 120 125
Gln Lys Phe Ser Thr Ala Lys Tyr Thr Lys Ser Val Thr Glu Ser Ile
130 135 140
Ala Thr Ala Thr Thr Lys Gly Phe Lys Val Gly Gly Ser Gly Asp Gly
145 150 155 160
Ser Asn Ile Phe Thr Ile Pro Leu Leu Leu Asn Asn Gly Ile Lys Ile
165 170 175
Asn Gly Glu Phe Asn Ser Ser Thr Thr Glu Thr Lys Thr Lys Ser Glu
180 185 190
Thr Arg Ser Ile Glu Ala Ser Ala Gln Ser Ile Glu Val Pro Pro His
195 200 205
Lys Lys Tyr Lys Ala Asp Val Val Leu Glu Gln Arg Asn Phe Trp Gly
210 215 220
Asp Val Thr Phe Thr Gly Val Gly Val Lys Pro Ala Thr Thr Ile Lys
225 230 235 240
Gly Thr Ala Ser Tyr Trp Ala Pro Asn Gly Met Gly Ala Trp Lys Gln
245 250 255
Tyr Thr Phe Ser Asp Asn Thr Val Asn Tyr Trp Lys Gly Leu Thr Ala
260 265 270
Ser Gln Lys Asn Ser Ile Asn Gly Ile Lys Phe Glu Asn Asn Asp Val
275 280 285
Lys Ala Glu Gly Thr Ala Lys Val Glu Gly Thr Phe Gly Ser Met Leu
290 295 300
Ile Val Lys Val Tyr Asp Ile Thr Asp Lys Ser Asn Pro Lys Leu Val
305 310 315 320
Glu Thr Arg Ser Phe Lys
325
<210> 117
<211> 630
<212> PRT
<213> 芽孢杆菌属的种
<400> 117
Met Asn Lys Asn Ser Asn Asn Asn Glu Phe Thr Asn Asn Asn Gly Met
1 5 10 15
Val Ser Gln Pro Lys His Pro Phe Val Lys Glu Pro Gly Ser Glu Leu
20 25 30
Gln Asn Met Asn Thr Val Gly Ala Pro Ile Ala Leu Ser Ser Thr Thr
35 40 45
Thr Arg Asp Gly Val Ile Ile Ala Thr Gly Ile Gly Trp Ala Ile Leu
50 55 60
Gly Phe Val Pro Val Val Gly Pro Gly Leu Ser Ala Ala Ala Gly Leu
65 70 75 80
Leu Asn Val Ile Val Pro Phe Leu Trp Pro Glu Asp Asp Ala Gly Ser
85 90 95
Ser Gln Ile Ser Trp Glu Gln Leu Met Asp Ser Val Glu Glu Leu Val
100 105 110
Asp Glu Arg Ile Leu Thr Ser Lys Arg Ser Asp Ala Thr Ala Arg Leu
115 120 125
Glu Gly Ile Lys Ala Leu Val Asn Glu Tyr Thr Gln Ala Ile Asp Asp
130 135 140
Leu Lys Ala Asp Pro Asn Asn Glu Leu Lys Lys Asp Arg Val Arg Asp
145 150 155 160
Lys Phe Asp Asp Val Glu Asp Tyr Val Lys Val Ser Met Pro Phe Phe
165 170 175
Arg Ala Gln Gly Phe Glu Val Pro Met Leu Ala Met Tyr Ala Gln Ala
180 185 190
Ala Asn Ile His Leu Leu Leu Leu Arg Asp Val Val Gln His Gly Ala
195 200 205
Ser Trp Gly Phe Gln Gln Tyr Glu Ile Asp Arg Tyr Tyr Asn His Thr
210 215 220
Gly Leu Thr Gly Ser Pro Gly Met Leu Gln Leu Leu Ala Gln Tyr Thr
225 230 235 240
Ala His Cys Val Arg Trp Tyr Asn Thr Gly Leu Glu Asn Arg Arg Ala
245 250 255
Thr Asn Asp Trp Asn Lys Phe Asn Asp Phe Arg Arg Asp Met Thr Leu
260 265 270
Met Val Leu Asp Ile Ile Ser Leu Trp Pro Thr Tyr Asp Pro Lys Phe
275 280 285
Tyr Ala Val Pro Thr Lys Ser Gln Leu Thr Arg Thr Val Tyr Thr His
290 295 300
Trp Ile Gly His Leu Arg Asn Arg Trp His Gly Asn Pro Asp Ala Leu
305 310 315 320
Ser Leu Ser Val Val Glu Asn Ser Ile Val Pro Ala Pro His Leu Phe
325 330 335
Thr Trp Leu Arg Glu Val Trp Phe Tyr Phe Thr Gly Gly Gly Gly Pro
340 345 350
Ser Ala Glu Thr Leu Phe Thr Gly Phe Arg Pro Phe Phe Gln Thr Thr
355 360 365
Leu Ser Asp Asn Leu Leu Thr Gly Glu Leu Gln Gly Asn Tyr Gly Thr
370 375 380
Glu Pro Arg Gln Asp Ile Thr Ile Pro Ser Pro Gln Ser Gly Asp Asp
385 390 395 400
Ile Trp Lys Val His Thr Ser Ser Ala Leu Met Ala Glu Gly Pro Glu
405 410 415
His Arg Arg Leu Lys Gly Trp Trp Phe Leu Phe Thr Asn Ser Pro Thr
420 425 430
Gln Gln Ala His Thr Lys Thr Gly Tyr Gly Gln Gln Leu Ile Tyr Thr
435 440 445
His Leu Gly Leu Pro Cys Lys Asn Ser Cys Gly Asp Glu Asn Pro Asn
450 455 460
Leu Ser Asn Pro Cys Asp Asn Ser Ser Leu Tyr Ser His Arg Leu Ser
465 470 475 480
Asn Phe Arg Gly Asn Phe Asp Pro Thr Tyr Cys Ser Tyr Gly Trp Thr
485 490 495
His Val Ser Ala Asp His Asn Asn Leu Ile Asp Ala Gln Lys Ile Thr
500 505 510
Gln Ile Pro Ala Val Lys Ala Ser Ser Ile Ser Gly Gly Ala Arg Val
515 520 525
Val Lys Gly Pro Gly Ser Thr Gly Gly Asp Leu Val Glu Leu Asp Phe
530 535 540
Ser Thr Ala Ser Ser Leu Arg Leu Lys Val Thr Leu Ala Asp Thr Thr
545 550 555 560
Lys Ser Tyr Gln Ile Arg Thr Arg Ser Ala Tyr Gly Ser Val Ser Lys
565 570 575
Ser Ala Asp Thr Leu Thr Tyr Ser Asp Phe Ser Tyr Ala Asn Ser Thr
580 585 590
Ile Ile Pro Pro Arg Asp Ser Ser Glu Tyr Thr Tyr Ile Met Glu Leu
595 600 605
Asp Pro Ser Asp Phe Gly Gly Leu Glu Gly Lys Phe Phe Ile Asp Lys
610 615 620
Ile Glu Phe Ile Pro Met
625 630
<210> 118
<211> 373
<212> PRT
<213> 芽孢杆菌属的种
<400> 118
Val Gly Lys Ser Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys
1 5 10 15
Asn Lys Asn Ser Arg Lys Tyr Leu Asp Ile Glu Gly Asn Gln Thr Gly
20 25 30
Asn Asn Ala Asn Val Gln Gln Tyr Glu Tyr Leu Ala Asp Ala Leu Ser
35 40 45
Glu Arg Phe Phe Leu His Lys Leu Asp Asp Asn Tyr Tyr Ala Ile Ile
50 55 60
Asn Leu Asn Ser Gly Lys Val Ile Asp Ile Arg Gly Asn Gln Thr Gly
65 70 75 80
Asn Asn Ala Asn Ile Gln Gln Phe Glu Trp Arg Gly Asp Ala Asn Ser
85 90 95
Glu Tyr Trp Tyr Phe Arg Arg Glu Glu Asp Gly Tyr Tyr Val Ile Glu
100 105 110
Ser Lys His Ser Gly Lys Val Ile Asp Ile Glu Gly Asn Gln Thr Gly
115 120 125
Asn Asn Ala Asn Val Gln Gln Phe Gln Phe Leu Lys Asp Ala Pro Ser
130 135 140
Glu Arg Phe Ala Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile
145 150 155 160
Asn Thr Gln Pro Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp
165 170 175
Gln Leu Pro Glu Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val
180 185 190
Pro Ala Ile Ala Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys
195 200 205
Gln Ile Lys Glu Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp
210 215 220
Lys Lys Gln Glu Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Asn Phe
225 230 235 240
Glu Val Thr Thr Gly Ile Arg Val Thr Asp Gln Glu Thr Ala Thr Lys
245 250 255
Thr Val Ser Leu Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly
260 265 270
Val Ser Leu Gly Met Ser Ser Gln Tyr Ser Gln Glu Leu Gln Thr Ser
275 280 285
Ile Ser His Thr Thr Glu Gln Leu Lys Glu Glu Thr Lys Arg His Thr
290 295 300
Ile Thr Asn Leu Phe Ser Glu Arg Met Ala Tyr Ser Arg Tyr Ile Leu
305 310 315 320
Thr Thr Glu Tyr Ser Val Arg Arg Asp Ser Gly Thr Ile Val Asn Ser
325 330 335
Ser Trp Thr Met Thr Asp Lys Thr Arg Thr His Ala Val Thr Phe Pro
340 345 350
Lys Ser Thr Gly Lys Val Leu Asn Glu Asn Ile Lys Gln Ile Ser Lys
355 360 365
Ser Glu Ser Leu Lys
370
<210> 119
<211> 503
<212> PRT
<213> 芽孢杆菌属的种
<400> 119
Leu Met Lys Tyr Lys Asp Arg Lys His Ala Lys Arg Lys Tyr Lys Gln
1 5 10 15
Ala Leu Leu Ala Thr Val Ala Thr Leu Thr Leu Gly Val Ser Thr Leu
20 25 30
Glu Ser Thr Ala Ser Ala Phe Ala Glu Glu Lys Asp Lys Asn Ala Pro
35 40 45
Gln Gln Gln Asn Thr Ile Gln Gln Lys Asp Ala Gly Thr Tyr Tyr Glu
50 55 60
Gly Glu Gln Lys Glu Leu Asn Pro Leu Ala Gln Leu Asp Lys Trp Thr
65 70 75 80
Gln Asp Leu Gly Lys Thr Ser Gly Ala Gly Asp Tyr Lys Thr Thr Leu
85 90 95
Gly Met Val Glu Lys Leu Leu Pro Thr Ile Tyr Lys Asp Leu Asn Ser
100 105 110
Gly Asn Phe Asn Asn Thr Ala Arg Ser Phe Thr Met Leu Ser Thr Ala
115 120 125
Leu Ile Pro Tyr Gly Gly Ala Phe Ile Ser Pro Ile Val Gly Leu Ile
130 135 140
Trp Pro Glu Asn Thr Thr Ala Gln Asp Ala Lys Leu Gln Lys Met Val
145 150 155 160
Glu Gly Leu Thr Ser Met Met Gly Gln Lys Ile Asn Asp Tyr Asp Leu
165 170 175
Ala Lys Leu Lys Gln Lys Thr Asp Ala Leu Thr Asn Thr Leu Lys Lys
180 185 190
Phe Gln Asp Ser Val Asn Asn Lys Pro Gln Ser Glu Ser Tyr Tyr Asp
195 200 205
Asn Ile Gln Thr Ala Asn Val Thr Tyr Ala Asp Tyr Ile Asn Thr Lys
210 215 220
Phe Glu Glu Ile Ile Lys Glu Ser Gln Thr Pro Gly His Gln Ile Pro
225 230 235 240
Glu Leu Pro Ile Phe Thr Val Ile Ala Thr Ala His Leu Gln Phe Leu
245 250 255
His Phe Ile Glu Glu Asn Ala Val Lys Asn Pro Arg Ile Lys Met Asp
260 265 270
Pro Glu His Leu Asn Arg Tyr Phe Pro Lys Phe Lys Asp Thr Ala Arg
275 280 285
Lys Tyr Arg Lys Tyr Ile Asn Asp Val Asn His Asp Ala Gln Gln Trp
290 295 300
Leu Tyr Asp Phe Thr Gly Gly Gly His Ile Lys Ala Asn Asn Pro Ala
305 310 315 320
His Ile Glu Ala Met Asn Lys Ile Ala Asp Leu Thr Lys Gln Thr Ser
325 330 335
Asp Asn Glu Ala Phe Leu Leu Ala Ser Asn Pro Pro Ser Val Gly Asp
340 345 350
Lys Thr Gly Leu Val Lys Glu Asp Gly Asp Lys Trp His Tyr Tyr Ser
355 360 365
Pro Arg Asp Gly Phe Glu Asn Ser Gln His Gln Lys Phe Asn Lys Gly
370 375 380
Ser Leu Val Thr Gly Pro Leu Asp Ile Asp Gly Arg Thr Tyr Leu Phe
385 390 395 400
Ser Phe Glu Glu Gly Met Lys Asn Ser Ala Gly Lys Lys Phe Asn Lys
405 410 415
Gly Asp Met Phe Thr Gly Trp Tyr Asn Asp Ala Gly Val Trp Ser Tyr
420 425 430
Tyr Lys Pro Glu Ser Gly Val Met Ala Thr Asp Trp Gln Asn Ile Ser
435 440 445
Gly Lys Trp Tyr Tyr Phe Ala Thr Ala Tyr Gln Glu Phe Asn Gly Gly
450 455 460
Ala Phe Ile Ser Lys Gly Asp Met Ile Lys Gly Lys Thr Val Glu Ile
465 470 475 480
Ser Gly Lys Leu Tyr Thr Phe Asp Glu Asn Gly Ala Cys Leu Asn Pro
485 490 495
Asp Gly Lys Pro Ile Ala Lys
500
<210> 120
<211> 722
<212> PRT
<213> 芽孢杆菌属的种
<400> 120
Met Tyr Leu Ile Met Tyr Asn Asn Glu Glu Gln Pro Ile Ala Asn Arg
1 5 10 15
Lys Ser Asn Pro Asn Val Glu Asn Asp Cys Arg Cys Ser Glu Asp Asn
20 25 30
Tyr Asn Leu Leu Gln Glu Glu Pro Thr Leu Ser Gln Val Ser Ile Ala
35 40 45
Asp Thr Lys Asn Leu Tyr Thr Ser Leu Leu Leu Ala Glu Asn Gly Gly
50 55 60
Glu Leu Gln Pro Lys Val Pro Asp Phe Pro Thr Tyr Val Thr Thr Pro
65 70 75 80
Asn Asn Glu Asp Ser Lys Ile Cys Val Lys Asp Ile Thr Cys Val Trp
85 90 95
Gly Lys Trp Gly Glu Asn Arg Gln Gly Phe Leu Glu Pro Ala Val Arg
100 105 110
Ile Thr Pro Val Ala Gly Ala Ile Gly Ser Phe Leu Leu Gln Lys Ile
115 120 125
Gly Gly Leu Ile Ala Thr Lys Leu Ile Asn Gly Leu Tyr Gly Ile Leu
130 135 140
Phe Pro Thr Pro His Pro Ile Thr Leu Glu Gln Ile Leu Glu Ala Val
145 150 155 160
Ser Glu Leu Leu Asn Gln Arg Leu Asp Thr Glu Asn Tyr Asn Arg Val
165 170 175
Ser Leu Glu Leu Glu Gly Leu Gln Asn Ser Ser Lys Ala Phe Ile Glu
180 185 190
Thr Val Glu Ala Tyr Glu Gln Lys Ile Tyr Thr Asp Pro Leu Tyr Ala
195 200 205
Glu Thr His Ala Asp Glu Ile Arg Asn Ala Met Asn Thr Phe His Thr
210 215 220
Leu Cys Val Asn Arg Met Pro Gln Phe Arg Leu Pro Gly Tyr Gln Ile
225 230 235 240
Leu Leu Leu Pro Leu Tyr Ala Gln Ala Ala Asn Ile His Ile Gly Phe
245 250 255
Leu Lys Asp Val Leu Asp Asn Gln Thr Lys Trp Gly Leu Ser Ala Ala
260 265 270
Asp Val Lys Leu Tyr Thr Asn Arg Phe Leu Asp Val Ile Gly Ile Tyr
275 280 285
Ser Thr Tyr Cys Asn Thr Thr Tyr His Thr Asp Phe Ile Lys Gln Phe
290 295 300
Ser Asp Asp Phe Lys Arg Pro Leu Asp Tyr Arg Thr Phe Met Ile Leu
305 310 315 320
Asn Val Leu Asp Tyr Val Ser Leu Trp Ser Met Leu Arg Phe Asn Lys
325 330 335
Leu Glu Val Leu Thr Ser Ala Pro Leu Tyr Glu Tyr Gly Arg Ala Thr
340 345 350
Pro Asn Gly Ile Ile Pro Lys Tyr Ser Tyr Ser Phe Trp Asp Asn Leu
355 360 365
Asn Arg Leu Phe Gln Gly Phe Pro Asn Arg Val Leu Asp Arg Val Val
370 375 380
Ser Gln Asn Lys Leu Ile Phe Ser Asn Arg Pro Pro Thr Leu Tyr Asn
385 390 395 400
Tyr Leu Asn Arg Ser Tyr Ser Ile Thr Thr Tyr Tyr Ser Gly Asn Lys
405 410 415
Ser Phe Lys Val Gly Tyr Asn Thr Tyr Ser Phe Cys Asn Gly Asn Asp
420 425 430
Arg Cys Glu Tyr Tyr Thr Ser Ser Ile Asn Ala Lys Asn Leu Lys Glu
435 440 445
Ala Ser Gln Ile Ser Asn Leu Tyr Thr Tyr Ser Gln Gly Trp Pro Asn
450 455 460
Val Arg Leu Glu Val Arg Asp Trp His Asp Met Arg Tyr Arg Asp Gly
465 470 475 480
Asp Arg Val Glu Ala Thr Ile Ala Ser Asp Pro Tyr Ser Tyr Asn Trp
485 490 495
Val Ser Tyr Pro Glu Arg Thr Ile Gln Ser Ile Ile Gly Leu Pro Pro
500 505 510
Ser Met Leu Asn Glu His Ile Asn Ile Ile Asp Gly Pro Gln Asn Arg
515 520 525
Val Gln Ser Asp Gly Val Ile Ala Ala Asn Ile Thr Ser Phe Gln Arg
530 535 540
Lys Ala Ala Lys Thr Tyr Ala Val Ser Asn Gly Thr Ile Arg His Pro
545 550 555 560
Phe Thr Ala Gly Lys Gly Leu Ala Ile Ser Pro Leu Glu Phe Tyr Asp
565 570 575
Thr Tyr Asn Val Gly Ser Asn Ala Thr Lys Lys Arg Glu Phe Tyr Ile
580 585 590
Val Gln Arg Tyr Gly Asn Asn Gly Asp Ala Val Arg Ile Pro Ser Pro
595 600 605
Lys Ser Phe Gly Ser Asn Phe His Gly Leu Ile Tyr His Ile Phe Asn
610 615 620
Pro Tyr Asp Val Ser Phe Ser Leu Asp Val Tyr Leu Lys Val Ser Ala
625 630 635 640
Pro Asn Arg Thr Ser Val Gly Val Tyr Leu Asn Gly Val Gly Ala Thr
645 650 655
Lys Glu Ile Tyr Thr Ala Thr Asp Asn Asp Gly Val Thr Asp Asn Asp
660 665 670
Ser Glu Ser Lys Ile Val Lys Phe Leu Ser Gly Phe Asn Leu Lys Pro
675 680 685
Gly Asn Val Glu Leu Tyr Ile Glu Asn Ser Gly Ser Ala Asp Leu Phe
690 695 700
Val Glu Asn Ile Ile Val Val Pro Ala Gly Ser Thr Phe Ile Ser Arg
705 710 715 720
Ile Asn
<210> 121
<211> 1888
<212> PRT
<213> 芽孢杆菌属的种
<400> 121
Met Leu Asn Tyr Ile Ser Leu Glu Arg Gly Gly Phe Met Ala Ser Leu
1 5 10 15
Ala Asp Leu Tyr Pro Ser Tyr Tyr Asn Val Leu Ala Glu Pro Pro Phe
20 25 30
Ile Asn Gly Asp Asn Asp Ile Ile Ile Thr Ala Lys Ser Asp Cys Lys
35 40 45
Glu Asp Ser Ala Ser Glu Ala Ala Gly Asn Ser Asn Lys Asn Asn Tyr
50 55 60
Trp Ala Gly Ala Gln Ser Thr Trp Lys Cys Tyr Trp Gln Ser Gly Tyr
65 70 75 80
Gln Asp Ala Tyr Thr Gly Val Ile Ser Ala Ala Phe Ser Asp Asp Asn
85 90 95
Phe Asn Tyr Val Asp Phe Thr Lys Lys Val Ile Gly Leu Asn Gly Phe
100 105 110
Phe Ile Ser Trp Leu Gly Gly Glu Ile Ser Glu Glu Leu Arg Ser Thr
115 120 125
Leu Asn Gly Ala Glu Lys Ala Leu Glu Val Ala Gly Met Val Thr Leu
130 135 140
Pro Ile Val Val Pro Ile Ala Gln Ala Leu Val Met Ala Ile Phe Pro
145 150 155 160
Ile Ser Val Thr Lys Lys Lys Arg Glu Asp Gln Ala Lys Thr Leu Trp
165 170 175
Asp Leu Leu Ala Lys Arg Val Asp Glu Glu Ile Lys Asp Leu Ile Lys
180 185 190
Asp Tyr Asp Ala Asn Ala Leu Asn Gln Phe Ile Ala Gly Phe Gln Thr
195 200 205
Val Leu Lys Asp Leu Ser Asp Ala Val Gln Ile Ala Ile Cys Gln Pro
210 215 220
Ile Asp Leu Asn Pro Asp Ile Pro Ser Lys Val Asn Pro Asn Cys Ile
225 230 235 240
Pro Tyr Thr Asp Ile Glu Leu Val Asn Asn Val Lys Gln Lys Leu Ser
245 250 255
Thr Val Asp Ser Ala Phe Thr Thr Leu Ile Pro Gln Phe Gln Lys Ser
260 265 270
Asp Tyr Ala Ser Ala Glu Leu Pro Gln Phe Cys Leu Ala Ala Thr Leu
275 280 285
His Leu Ser Thr Leu His Ser Ala Ile Ile Met Ser Glu Pro Trp Glu
290 295 300
Leu Asn Pro Asn Ile Arg Lys Ser Tyr Ile Arg Lys Leu Arg Arg Leu
305 310 315 320
Ile Lys Ser Tyr Ser Leu Tyr Val Thr Gln Val Val Thr Lys Ala Ser
325 330 335
Gln Asp Tyr Leu Lys Gln Asn Ser Ala Gly Asn Leu Thr Lys Lys Gly
340 345 350
Phe Asn Glu Tyr Asn Gln Leu Leu Ser Phe Tyr Val Thr Glu Cys Leu
355 360 365
Asp Tyr Val Ala Met Trp Arg Phe Leu Asp Pro Ile Asp Tyr Pro Leu
370 375 380
Pro Thr Tyr Ala Glu Leu Thr Arg Val Ile Val Gly Asn Ile Val Gly
385 390 395 400
Pro Tyr Glu Glu Asp Asn Thr Val Gly Gly Asn Ala Thr Pro Ile Ser
405 410 415
Asp Asp Leu Arg Pro Lys Ile Gln Ile Phe Asp Pro Thr Ser Gln Asp
420 425 430
Phe Lys Glu Leu Ser Asn Ser Arg Met Tyr Asp Leu Leu Gly Leu Thr
435 440 445
Tyr Asn Asn Leu Gln Met Lys Asn Val Thr Met His Ser Gln Gly Asp
450 455 460
Ser Arg Asn Val Val Val Ala Gly Glu His Leu Glu Tyr Met Tyr Tyr
465 470 475 480
Gln Asp Ser Leu Thr Lys Asp Phe Gly Asn Thr Thr Gly His Ser Val
485 490 495
Phe Ser Asp Val Ser Leu Asn Pro Ala Thr Tyr Leu Asn Met Glu Ser
500 505 510
Ala Tyr Asn Asn Val Asn Asn Val Asp Gln Thr Glu Leu His Ile Cys
515 520 525
Lys Met Asn Gly Asp Ile Asp Ile Val Gly Ala Val Asp Phe Glu Thr
530 535 540
Cys Asn Ala His Gly Gly Lys Gly Leu Gly Thr Arg Cys Thr Ser Asn
545 550 555 560
Asn Lys Pro Leu Gly Ile Asp Lys Ile Gln Met Ile Tyr Pro Val Thr
565 570 575
Val Glu Ser Ile Ala Gly Asn Gln Ala Lys Met Gly Phe Val Ala Thr
580 585 590
His Val His Asp Ile Val Thr Pro Glu Asn Ile Ile Gly Gln Lys Met
595 600 605
Gln Asp Asn Asp Gly Asn Asp Thr Asp Gln Pro Leu Leu Gln Ile Arg
610 615 620
Thr Ile Ser Val Glu Lys Gly Lys Leu Ser Gly Asn Ala Arg Pro Ile
625 630 635 640
Lys Glu Tyr Val Thr Gly Ser Asn Pro Val Leu Ile Pro Pro Gly Gly
645 650 655
Ser Ile Asp Leu Thr Cys Thr Asn Leu Thr Ala Gln Lys Tyr Phe Ile
660 665 670
Arg Phe Cys Leu Ser Thr Met Ser Asp Ala Leu Ser Leu Gln Trp Gln
675 680 685
Leu Asn Asn Gly Gln Thr Gln Val Val Ser Ile Pro Thr Ala Thr Ser
690 695 700
Asn Phe Ile Ser Asn Asp Gln Lys Gly Ile Ser Gly Leu Ser Leu Asn
705 710 715 720
Phe Gly Pro Tyr Thr Ala Asp Asn Leu Tyr Ala Val Phe Pro Glu Thr
725 730 735
Thr Phe Asp Asp Asn Gly Leu Pro Ile Leu Pro Asp Glu Ser His Ala
740 745 750
Val Glu Ile Gly Asp Gly Glu Phe Thr Val Thr Ile Thr Asn Pro Ala
755 760 765
Pro Ser Val Ser Asn Asp Ser Asn Asp Ile Thr Val Asp Arg Ile Glu
770 775 780
Phe Ile Pro Tyr Ile Glu Gln Pro Ala Pro Glu Leu Gln Lys Tyr Leu
785 790 795 800
Met Asp Thr Leu Ser Asn Ile Gln Pro Phe Thr Val Gly Leu Asn Gln
805 810 815
Pro Thr Ile Ile Trp Ser Ser Thr Asp Arg Val Ala Thr Glu Leu Asp
820 825 830
Met Arg Tyr Glu Gly Tyr His Pro Ser Phe Ile Asn Ser Asn Pro Arg
835 840 845
Leu Asp Phe Tyr Tyr Asn Asn Val Leu Gln Thr Ala Ile Arg Ser Glu
850 855 860
Thr Pro Asp Ile Tyr Gly Tyr Tyr Asn Tyr Thr Ile Glu Gly Asn Asp
865 870 875 880
Thr Asp Lys Thr Gly Gly Phe Asp Arg Val Glu Leu Ile Ala Asn Thr
885 890 895
Thr Asn Leu Glu Thr Ile Thr Leu Ser Gly Lys Val Tyr Gly Pro Asn
900 905 910
Gln Gln Tyr Lys Pro Thr Pro Pro Pro Thr Pro Pro Thr Pro Pro Thr
915 920 925
Pro Ala Ala Thr Ile Val Asp Ser Val Pro Phe Val Val Gly Lys Gly
930 935 940
Ala Asn Lys Thr Ile Trp Ser Ser Thr Ser Ile Val Ala Asn Gln Ala
945 950 955 960
Asn Leu Lys Leu Val Ala Tyr Gln Pro Asp Phe Ile Thr Tyr Asp Tyr
965 970 975
Gln Phe Ala Phe Tyr Tyr Asn Gly Val Leu Gln Asn Ser Ile Gln Thr
980 985 990
Arg Asp Pro Lys Gly Phe Asp Met Tyr Ala Val Ser Ser Gly Phe Asn
995 1000 1005
Ser Ile Ala Leu Ile Ser Lys Ala Pro Asn Phe Glu Thr Val Arg
1010 1015 1020
Leu Ser Gly Asn Ile Tyr Thr Pro Gln Thr Pro Pro Pro Pro Pro
1025 1030 1035
Pro Pro Thr Pro Leu Pro Ala Ala Thr Ile Val Asp Ser Val Pro
1040 1045 1050
Phe Val Val Gly Lys Gly Ala Asp Thr Thr Ile Trp Arg Ser Thr
1055 1060 1065
Ser Ile Val Ala Asn Gln Ala Asn Leu Lys Leu Val Ala Tyr Gln
1070 1075 1080
Pro Asp Phe Leu Thr Ser Glu His Gln Phe Ala Phe Tyr Tyr Asn
1085 1090 1095
Asp Val Leu Gln Lys Val Thr Leu Thr Gly Gln Asp Ser Lys Gly
1100 1105 1110
Phe Asp Ile Tyr Ser Val Ser Ser Gly Phe Asn Ser Ile Ala Leu
1115 1120 1125
Ile Ser Gln Val Pro Asn Phe Glu Thr Val Arg Leu Ser Gly Asp
1130 1135 1140
Ile Tyr Ala Pro Gln Ile Thr Pro Lys Pro Pro Thr Leu Pro Glu
1145 1150 1155
Ser Val Gly Gly Phe Ser Thr Pro Lys Ser Leu Asp Ile Pro Val
1160 1165 1170
Asn Thr Ser Ser Glu Val Trp Arg Asp Tyr Thr Val Tyr Gly Ile
1175 1180 1185
Gln Ala Lys Ile Asn Thr Arg Phe Leu Asp Ser Pro Asp Arg Asn
1190 1195 1200
Ile Thr Tyr Trp Tyr Gln Phe Tyr Leu Thr Ser Gly Glu Gly Gln
1205 1210 1215
Asn Asp Thr Leu Val His Glu Glu Lys Ala Lys Ser Gly Asp Tyr
1220 1225 1230
Glu Tyr Ile Val Asn His Tyr Phe Asn Lys Ile Val Leu His Val
1235 1240 1245
Thr Gly Phe Tyr Pro Val Ser Glu Pro Ser Thr Ser Ser Val Thr
1250 1255 1260
Ile Glu Ser Cys Ala Ile Tyr Gly Tyr Glu Arg Gln Lys Ile Asp
1265 1270 1275
Pro Ser Val Asn Lys Thr Ile Glu Pro Met Asp Thr Phe Gly Arg
1280 1285 1290
Lys Ser Ile Glu Ile Trp Thr Pro Thr Lys Pro Thr Ala Lys Gly
1295 1300 1305
Phe Glu Ala Ile Leu Gly Val Asp Val Ile Thr Met Gly Ala Trp
1310 1315 1320
Ser Ser Asp Thr Val Tyr Gln Phe Tyr Leu Asn Gly Gln Leu Val
1325 1330 1335
Thr Glu Gln Pro Val Asp Phe Thr Asn Leu Met Lys Asn Val Tyr
1340 1345 1350
Glu Val Pro Gln Gly Phe Asn Ser Ile Ser Ile Phe Asp Pro Asn
1355 1360 1365
Ala Tyr Asp Pro Asp Asp Pro Asp Asp Asn Gly Asn Tyr Pro Leu
1370 1375 1380
Thr Gly Asp Glu Tyr Asn Ile Thr Ile Ile Gly Thr Ile Phe Gly
1385 1390 1395
Asp Ala Ser Ser Ser Asn Leu Pro Pro Glu Asn Pro Val Asp Gly
1400 1405 1410
Thr Met Phe Thr Thr Arg Glu Ala Phe Met Thr Ile Ala Arg Leu
1415 1420 1425
Val Tyr Ala Leu Phe Thr Thr Ser Ser Gln Thr Thr Leu Asn Leu
1430 1435 1440
Thr Ile Thr Asp Tyr Trp Ile His Gln Leu Val Met Lys Val Asn
1445 1450 1455
Lys Leu Ser Asn Glu Lys Phe Gly Ile Glu Lys Lys Glu Leu Leu
1460 1465 1470
Lys Leu Leu Ser Lys Ala Lys Gln Ile Leu Lys Gly Lys Asn Phe
1475 1480 1485
Leu Arg Asn Gly Ser Phe Asp Thr Leu Asp Asn Trp Gln Val Gly
1490 1495 1500
Arg Glu Val Ala Ala Ile Tyr Gly His Glu Leu Phe Gln Gly Asn
1505 1510 1515
Tyr Leu Phe Leu Pro Pro Pro Thr Leu Ala Pro Ser Tyr Ala Tyr
1520 1525 1530
Gln Arg Ile Pro Glu Ser Arg Leu Lys Lys Asn Thr Arg Tyr Lys
1535 1540 1545
Ile Ser Gly Phe Ile Ala Ser Ser Leu Glu Leu Glu Leu Ile Val
1550 1555 1560
Ser Arg Tyr Gly Lys Glu Ile Val Lys Thr Leu Asn Val Pro Tyr
1565 1570 1575
Gly Lys Ala Leu Pro Val Ser Pro Asp Ala His Pro Asn Cys Cys
1580 1585 1590
Asn His Asn Leu Cys Gly Cys Pro Ser Cys Ile Gly Asn Gly Thr
1595 1600 1605
Asn Lys His Phe Phe Asn Tyr Cys Ile Asp Val Gly Thr Val Gln
1610 1615 1620
Thr Ile Thr Asp Leu Gly Ile Glu Phe Gly Val Arg Ile Lys Lys
1625 1630 1635
Gly Asn Gly Tyr Ala Lys Leu Gly Tyr Leu Glu Ile Gln Glu Glu
1640 1645 1650
Arg Pro Leu Thr Pro Gln Glu Val Gln Asn Val Gln Gln Lys Glu
1655 1660 1665
Leu Lys Trp Lys Glu Thr Phe Leu Gln Glu Lys Gln Gln Ile Thr
1670 1675 1680
Asn Leu Leu Asn Pro Ile Ile Thr Lys Ile Asn Ala Phe Phe Lys
1685 1690 1695
Asn Lys Asp Trp Asn Ser Asp Ile Leu Pro His Val Thr Tyr Gln
1700 1705 1710
Asp Leu Ser Thr Ile Ile Ile Pro Lys Leu Pro Lys Met Thr His
1715 1720 1725
Trp Phe Met Thr Asp Arg Val Gly Glu His Tyr Glu Ile Met Gln
1730 1735 1740
Lys Leu Gln Gln Ala Leu Gln Arg Val Arg Thr His Leu Asp Ala
1745 1750 1755
Glu Asn Leu Ile His Asn Gly Asp Phe Thr Asp Gly Leu Gln Asn
1760 1765 1770
Trp Glu Ile Gln Gly Asp Val Ser Leu Phe Ala Pro Gly Ile Leu
1775 1780 1785
Gln Leu Ser Asp Trp Asp Ala Ser Val Ser Gln Thr Ile Glu Ile
1790 1795 1800
Leu Asn Phe Thr Ala Gly Gln Gly Tyr Thr Leu Leu Val Asn Ser
1805 1810 1815
Lys Gly Ala Gly Ser Ile Thr Ile Glu His Gly Gln Val Val Glu
1820 1825 1830
Thr Leu His Met Met Ser Pro Asn Phe Met Thr Lys Thr Leu Thr
1835 1840 1845
Phe Tyr Pro Val Ser Pro Thr Leu Glu Ile Gln Ile Leu Ser Asp
1850 1855 1860
Gly Ala Glu Leu Leu Val Asp Ser Val Thr Ile Leu Gln Asn Pro
1865 1870 1875
Ile Glu Asn Ser Asp Ala Thr Ser Cys Glu
1880 1885
<210> 122
<211> 788
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 122
Met Leu Asn Tyr Ile Ser Leu Glu Arg Gly Gly Phe Met Ala Ser Leu
1 5 10 15
Ala Asp Leu Tyr Pro Ser Tyr Tyr Asn Val Leu Ala Glu Pro Pro Phe
20 25 30
Ile Asn Gly Asp Asn Asp Ile Ile Ile Thr Ala Lys Ser Asp Cys Lys
35 40 45
Glu Asp Ser Ala Ser Glu Ala Ala Gly Asn Ser Asn Lys Asn Asn Tyr
50 55 60
Trp Ala Gly Ala Gln Ser Thr Trp Lys Cys Tyr Trp Gln Ser Gly Tyr
65 70 75 80
Gln Asp Ala Tyr Thr Gly Val Ile Ser Ala Ala Phe Ser Asp Asp Asn
85 90 95
Phe Asn Tyr Val Asp Phe Thr Lys Lys Val Ile Gly Leu Asn Gly Phe
100 105 110
Phe Ile Ser Trp Leu Gly Gly Glu Ile Ser Glu Glu Leu Arg Ser Thr
115 120 125
Leu Asn Gly Ala Glu Lys Ala Leu Glu Val Ala Gly Met Val Thr Leu
130 135 140
Pro Ile Val Val Pro Ile Ala Gln Ala Leu Val Met Ala Ile Phe Pro
145 150 155 160
Ile Ser Val Thr Lys Lys Lys Arg Glu Asp Gln Ala Lys Thr Leu Trp
165 170 175
Asp Leu Leu Ala Lys Arg Val Asp Glu Glu Ile Lys Asp Leu Ile Lys
180 185 190
Asp Tyr Asp Ala Asn Ala Leu Asn Gln Phe Ile Ala Gly Phe Gln Thr
195 200 205
Val Leu Lys Asp Leu Ser Asp Ala Val Gln Ile Ala Ile Cys Gln Pro
210 215 220
Ile Asp Leu Asn Pro Asp Ile Pro Ser Lys Val Asn Pro Asn Cys Ile
225 230 235 240
Pro Tyr Thr Asp Ile Glu Leu Val Asn Asn Val Lys Gln Lys Leu Ser
245 250 255
Thr Val Asp Ser Ala Phe Thr Thr Leu Ile Pro Gln Phe Gln Lys Ser
260 265 270
Asp Tyr Ala Ser Ala Glu Leu Pro Gln Phe Cys Leu Ala Ala Thr Leu
275 280 285
His Leu Ser Thr Leu His Ser Ala Ile Ile Met Ser Glu Pro Trp Glu
290 295 300
Leu Asn Pro Asn Ile Arg Lys Ser Tyr Ile Arg Lys Leu Arg Arg Leu
305 310 315 320
Ile Lys Ser Tyr Ser Leu Tyr Val Thr Gln Val Val Thr Lys Ala Ser
325 330 335
Gln Asp Tyr Leu Lys Gln Asn Ser Ala Gly Asn Leu Thr Lys Lys Gly
340 345 350
Phe Asn Glu Tyr Asn Gln Leu Leu Ser Phe Tyr Val Thr Glu Cys Leu
355 360 365
Asp Tyr Val Ala Met Trp Arg Phe Leu Asp Pro Ile Asp Tyr Pro Leu
370 375 380
Pro Thr Tyr Ala Glu Leu Thr Arg Val Ile Val Gly Asn Ile Val Gly
385 390 395 400
Pro Tyr Glu Glu Asp Asn Thr Val Gly Gly Asn Ala Thr Pro Ile Ser
405 410 415
Asp Asp Leu Arg Pro Lys Ile Gln Ile Phe Asp Pro Thr Ser Gln Asp
420 425 430
Phe Lys Glu Leu Ser Asn Ser Arg Met Tyr Asp Leu Leu Gly Leu Thr
435 440 445
Tyr Asn Asn Leu Gln Met Lys Asn Val Thr Met His Ser Gln Gly Asp
450 455 460
Ser Arg Asn Val Val Val Ala Gly Glu His Leu Glu Tyr Met Tyr Tyr
465 470 475 480
Gln Asp Ser Leu Thr Lys Asp Phe Gly Asn Thr Thr Gly His Ser Val
485 490 495
Phe Ser Asp Val Ser Leu Asn Pro Ala Thr Tyr Leu Asn Met Glu Ser
500 505 510
Ala Tyr Asn Asn Val Asn Asn Val Asp Gln Thr Glu Leu His Ile Cys
515 520 525
Lys Met Asn Gly Asp Ile Asp Ile Val Gly Ala Val Asp Phe Glu Thr
530 535 540
Cys Asn Ala His Gly Gly Lys Gly Leu Gly Thr Arg Cys Thr Ser Asn
545 550 555 560
Asn Lys Pro Leu Gly Ile Asp Lys Ile Gln Met Ile Tyr Pro Val Thr
565 570 575
Val Glu Ser Ile Ala Gly Asn Gln Ala Lys Met Gly Phe Val Ala Thr
580 585 590
His Val His Asp Ile Val Thr Pro Glu Asn Ile Ile Gly Gln Lys Met
595 600 605
Gln Asp Asn Asp Gly Asn Asp Thr Asp Gln Pro Leu Leu Gln Ile Arg
610 615 620
Thr Ile Ser Val Glu Lys Gly Lys Leu Ser Gly Asn Ala Arg Pro Ile
625 630 635 640
Lys Glu Tyr Val Thr Gly Ser Asn Pro Val Leu Ile Pro Pro Gly Gly
645 650 655
Ser Ile Asp Leu Thr Cys Thr Asn Leu Thr Ala Gln Lys Tyr Phe Ile
660 665 670
Arg Phe Cys Leu Ser Thr Met Ser Asp Ala Leu Ser Leu Gln Trp Gln
675 680 685
Leu Asn Asn Gly Gln Thr Gln Val Val Ser Ile Pro Thr Ala Thr Ser
690 695 700
Asn Phe Ile Ser Asn Asp Gln Lys Gly Ile Ser Gly Leu Ser Leu Asn
705 710 715 720
Phe Gly Pro Tyr Thr Ala Asp Asn Leu Tyr Ala Val Phe Pro Glu Thr
725 730 735
Thr Phe Asp Asp Asn Gly Leu Pro Ile Leu Pro Asp Glu Ser His Ala
740 745 750
Val Glu Ile Gly Asp Gly Glu Phe Thr Val Thr Ile Thr Asn Pro Ala
755 760 765
Pro Ser Val Ser Asn Asp Ser Asn Asp Ile Thr Val Asp Arg Ile Glu
770 775 780
Phe Ile Pro Tyr
785
<210> 123
<211> 674
<212> PRT
<213> 芽孢杆菌属的种
<400> 123
Met Asp Ser Asn Lys Gln Lys Glu Met Ser Glu Ile Thr Ser Ile Thr
1 5 10 15
Asn Asp Gly Asn Cys Asp Asp Thr Lys Gly Val Glu Thr Leu Glu Ile
20 25 30
Ala Glu Pro Leu Val Ser Tyr Ser Trp Thr Glu Lys Asn Leu Val Glu
35 40 45
Ile Glu Asp Glu Trp Lys Arg Trp Ser Asp Glu Asn Glu Phe Leu Ser
50 55 60
Ile Pro Pro Val Val Gly Thr Val Ser Ser Leu Ile Leu Lys Lys Ile
65 70 75 80
Ala Gly Met Ile Gly Asp Lys Leu Leu Lys Ser Leu Phe Glu Lys Leu
85 90 95
Phe Pro Thr Pro Asn Pro Val Thr Ile Leu Glu Ile Leu Glu Ala Val
100 105 110
Gln Lys Leu Leu Asn Glu Arg Leu Ser Gln Glu Ile His Gln Arg Ile
115 120 125
Thr Leu Glu Leu Glu Gly Leu Gln Gln Asn Ile Thr Asn Phe Leu Glu
130 135 140
Asp Val Glu Asp Phe Glu Gly Arg Ile Ile Val Asp Glu Leu Glu Asp
145 150 155 160
Gly Asn Gln Pro Leu Ala Ile Ile Asp Ser Ile His Thr Leu Asn Gln
165 170 175
Ile Phe Asp Asn Arg Met Pro Gln Phe Arg Asn Pro Asp Tyr Lys Val
180 185 190
Leu Leu Leu Pro Leu Tyr Ala Gln Ala Ala Asn Leu His Ile Ser Phe
195 200 205
Leu Lys Asp Val Val Asp Asn Ala Ser Lys Trp Gly Leu Thr Ala Ser
210 215 220
Gln Ile Lys Thr Tyr Thr Asn Arg Phe Leu Asp Lys Ile Ser Gln Tyr
225 230 235 240
Ser Asn Tyr Cys Ile Glu Thr Phe Gln Arg Asp Phe Gln Ser Lys Phe
245 250 255
Ser Gln Lys Leu Gln Asp Val Leu Lys Tyr Arg Asn Phe Met Thr Ile
260 265 270
Asn Val Leu Asp Tyr Val Ser Leu Trp Pro Met Leu Arg Phe Thr Lys
275 280 285
Pro Phe Asn Phe Arg Thr Ser Ser Asn Leu Tyr Glu Met Gly Pro Thr
290 295 300
Pro Gly Glu Asn Phe Ile Phe Pro Tyr Ser Ser Trp Ala Asn Phe His
305 310 315 320
Ile Ile Phe Gln Gly Phe Pro Asn Lys Glu Leu Gln Ser Ile Ser Ala
325 330 335
Thr Asn Arg Phe Tyr Arg Ala Tyr Lys Gln Ser Gly Gly Trp Ser Met
340 345 350
Leu Ala Ile Thr Glu Val Met Gly Asn Leu Thr Cys Asn Tyr Ser Gly
355 360 365
Gly Lys Ser Ile Lys Phe Gly Tyr Glu Ser Leu Pro Ser Cys Pro Asp
370 375 380
Asp Thr Gly Val Gln Arg Cys Asp Val Val Lys Glu Glu Ile Asn Asp
385 390 395 400
Phe Ser Arg Ser Gln Leu Asn Gln Val Leu Leu Ser Ser Ser Val Ser
405 410 415
Thr Thr Asn Pro Pro Tyr Met Ser Ala Lys Tyr Leu Gln Asn Val Lys
420 425 430
Leu Ser Asn Gly Thr Val Tyr Asn Leu Ile Asn Gly Glu Tyr Gly Ser
435 440 445
Trp Ile Ser Tyr Pro Asn Tyr Thr Ile Arg Asn Ile Ile Gly Trp Ala
450 455 460
Lys Phe Met Glu Ser Lys Ala Thr Asn Ile Ile Asp Gly Pro Ser Asn
465 470 475 480
Gln Tyr Glu Gly Ser Leu Arg Gly Asn Leu Ser Thr Asn Ile Val Ser
485 490 495
Phe Gln Arg Arg Glu Tyr Asp Pro Tyr Thr Ile Tyr Asn Gly Gly Lys
500 505 510
Arg His Thr Ile Pro Ser His Thr Arg Gly Ile Leu Ile Ser Pro Leu
515 520 525
Glu Tyr Ser Gly Thr Phe Asn Val Gly Thr Gly Thr Lys Gly Asn Phe
530 535 540
Val Ile His Glu Arg His Gly Asn Asn Gly Asp Ala Val Val Ile Pro
545 550 555 560
Lys Thr Asn Gly Gly Tyr Arg His Gly Phe Glu Tyr Ile Leu Phe Asn
565 570 575
Pro Tyr Gly Thr Asn Leu Asn Met Asp Ile Tyr Ile Lys Ile Ser Ala
580 585 590
Pro Val Lys Thr Lys Leu Val Tyr Leu Ile Asn Gly Ala Gly Leu Asn
595 600 605
Lys Glu Ile Val Thr Ser Thr Asp Asn Asp Gly Val Thr Asp Asn Gly
610 615 620
Gln Lys Ser Lys Ile Ile Lys Leu Tyr Ser Asn Lys Gln Leu Val Pro
625 630 635 640
Gly Asn Thr Thr Phe Ser Ile Gln Asn Gly Gly Leu Gly Asp Leu Tyr
645 650 655
Leu Glu Gly Ile Val Val Val Pro Asn Gly Ser Ser Leu Ile Ser Arg
660 665 670
Ile Gln
<210> 124
<211> 610
<212> PRT
<213> 芽孢杆菌属的种
<400> 124
Leu Met Lys Tyr Lys Glu Arg Lys Asp Ala Lys Arg Lys Tyr Lys Gln
1 5 10 15
Ala Leu Leu Thr Thr Val Ala Thr Met Thr Leu Gly Val Asn Thr Leu
20 25 30
Gly Asn Thr Ser Ser Thr Phe Ala Ala Glu Glu Asp Asn Ile Ser Val
35 40 45
Ile Ser Thr His Thr Ile Asn Asn Val Gly Thr Gln Thr Asp Ile Ser
50 55 60
Glu Glu Ala Lys Lys Lys Thr Phe Phe Ser Lys Val Ser Ala Asn Leu
65 70 75 80
Thr Thr Ala Thr Gly Lys Lys Ile Val Val Ala Gly Glu Lys Glu Leu
85 90 95
Leu Asn Met Ile Lys Thr Gln Lys Val Asp Phe Asn Asn Ala Ser Arg
100 105 110
Ala Leu Val Gly Ala Ile Ile Asp Asn Ala Val Pro Gly Gly Met Phe
115 120 125
Leu Ser Pro Leu Ile Asn Thr Ile Trp Pro Glu Asn Gly Asn Gly Glu
130 135 140
Ile Gln Gln Leu Arg Glu Glu Leu Met Lys Val Met Asp Glu Lys Ile
145 150 155 160
Thr Thr Ser His Met Asn Ser Leu Lys Glu Glu Phe Arg Ala Leu Asn
165 170 175
Thr Ile Gln Asn Lys Phe Glu Lys Ala Val Asn Glu Lys Asn Ser Gln
180 185 190
Asn Val Asp Ala Ser Thr Lys Arg Thr Arg Ser Gly Trp Ala Ser Gly
195 200 205
Ile Glu Gln Ala Tyr Met Arg Leu Leu Ala Leu Thr Ser Ser Asn Asp
210 215 220
Phe Lys Val Ala Asp Leu Pro Leu Tyr Thr Gln Ile Ala Thr Ala His
225 230 235 240
Ile Ala Phe Leu Thr Ser Leu Asp Thr Pro Glu Met Ala Ala Lys Val
245 250 255
Asp Ile Thr Thr Thr Gly Leu Lys Glu Phe Tyr Asn Lys Gly Glu Leu
260 265 270
Ser Lys Leu Ala Asn Glu Tyr Met Arg His Ile Thr Glu Thr Tyr Asp
275 280 285
Tyr Ala Lys Arg Glu Tyr Ile Asn Lys Ile Glu Gln Val Gly Val Ala
290 295 300
Phe Asp Pro Ser Asn Glu Ala Asn Gln Gln Ala Asp Leu Asn Asn Arg
305 310 315 320
Leu His Ser Ala Glu Lys Lys Leu Lys Asp Arg Glu Gly Val Leu Ser
325 330 335
Thr Asn Pro Ala Met Ile Trp Asp Asn Asp Pro Lys Tyr Lys Lys Asn
340 345 350
Lys Thr Glu Val Thr Tyr Leu Lys Asp Ala Lys Asp Ala Tyr Pro Thr
355 360 365
Leu Leu Lys Leu Tyr Lys Leu Thr Val Ser Asp Pro Asn Met Ala Glu
370 375 380
Leu Ser Lys Gly Thr Trp Met Glu Lys Ser Gly Lys Trp His Phe Thr
385 390 395 400
Asn Lys Asn Gly His Asp Met Leu Gly Trp Val Gln Asp Asn Gly Lys
405 410 415
Arg Tyr Tyr Ile Ser Pro Glu Asn Gly Thr Lys Asn Ser Ala Gly Asp
420 425 430
Thr Phe Asn Lys Gly Glu Met Val Thr Gly Trp Lys Glu Val Asp Gly
435 440 445
His Arg Tyr Tyr Phe Ser Pro Lys Lys Thr Asp Thr Gly Asn Asn His
450 455 460
Lys Pro Ile Ala Glu Gly Glu Met Val Thr Asp Trp Lys Glu Val Asp
465 470 475 480
Gly His Arg Tyr Tyr Phe Ser Pro Glu Asn Gly Thr Arg Gly Tyr Glu
485 490 495
Arg Pro Ala Asp Asn Glu Lys Glu Ser Pro Phe Lys Glu Phe Thr Lys
500 505 510
Gly Glu Met Met Thr Gly Phe Ile Gln Arg Gln Ser Asp Tyr Asn Trp
515 520 525
Phe Phe Leu Ser Pro Glu Lys Asn Thr Gln Asn Ser Asp Gly Thr Thr
530 535 540
Phe Val Glu Gly Glu Met Met Thr Ala Trp Tyr Ser Asn Pro Lys Asp
545 550 555 560
Asp Lys His Trp Tyr Tyr Phe Ser Thr Ser Asn Gln Pro Asp Asp Ser
565 570 575
Asn Ser Gln Arg Leu Glu Ser Gly Gln Met Ala Lys Tyr Leu Asn Ile
580 585 590
Gly Gly Tyr Asn Phe Asn Arg Tyr Gly Val Cys Thr Asn Pro Asn Tyr
595 600 605
Lys Lys
610
<210> 125
<211> 289
<212> PRT
<213> 芽孢杆菌属的种
<400> 125
Met Asn Tyr Ala Ala Phe Ser Thr His Glu Leu Gly Gly Lys Ser Ser
1 5 10 15
Pro Phe Leu Leu Ser Val Glu Glu Trp Arg Ala Phe Ser Asn Tyr Ile
20 25 30
Asn Gln Cys Val Ser Leu Pro Thr Asn Ile Asp Ser Ile Ile Asp Ala
35 40 45
Ile Asn Arg Val Lys Asn Gly Thr Leu Lys Gln Ser Leu Phe Asp Leu
50 55 60
Arg Asn Arg Gly Ser Ser Tyr Thr Arg Val Leu Thr Thr Ser Ile Glu
65 70 75 80
Met Thr Thr Lys Val Lys Glu Ala Ser Leu Phe Trp Gly Ala Gly Gly
85 90 95
Arg Val Gly Ile Ile Ser Leu Ala Gln Asn Ile Val Arg Tyr Ala Asp
100 105 110
Leu Ile Cys Ile Lys His Tyr Ala Asp Leu Asn Gln Val Leu Leu Glu
115 120 125
Ala Gln Lys Gly His Leu Ser Pro Asn Thr Lys Ser Lys Phe Ile Asn
130 135 140
Ile Cys Lys Asp Leu Ser Glu His Ala Leu Arg Tyr His Leu Glu Ser
145 150 155 160
Gln Thr Met Leu Thr Tyr Ile Ser Gly Phe Tyr Thr Val Ile Gly Gly
165 170 175
Cys Lys Asp Thr His Gln Asn Cys Gln Asp Thr Leu Phe Arg Val Pro
180 185 190
Met Tyr Asn Asn Arg Glu Tyr Glu Val Ile Ala His Thr Asp Ile Tyr
195 200 205
Leu Leu Arg Ser Ile Arg Asn Val Ile Pro Ser Glu Ala Tyr Ile Leu
210 215 220
Pro Val Ile Arg Lys Ile His Leu Ile Trp Ser Ala Ile Ser Tyr Asp
225 230 235 240
Leu Lys Glu Leu Gly Ala Asp Leu Asn Glu Asp Leu Val Ser Leu Pro
245 250 255
Ala Ile Ile Ala Ala Leu Glu Leu Glu Leu Ala Phe Glu Asn Trp Ser
260 265 270
Ala Ile Arg Gln Glu Ala Glu Asp Phe Tyr Lys Asn Ala Glu Asn Phe
275 280 285
Ser
<210> 126
<211> 407
<212> PRT
<213> 芽孢杆菌属的种
<400> 126
Met Gln Arg Lys Val Tyr Thr Lys Ile Ser Gly Cys Ser Arg Glu Ala
1 5 10 15
Ser Leu Cys Pro Asn Ser Pro Asp Ser Ile Val Lys Lys Ile Glu Ile
20 25 30
Lys Glu Val Asn Gln Met Lys Val Asn Lys Gln Gly Leu Pro Ala Glu
35 40 45
Ser Lys Lys Phe Gln Lys Ile Thr Phe Lys Leu Thr His Glu Gly His
50 55 60
Arg Val Leu Thr Ala Ala Lys Thr Lys Asp Gly Glu Gly Leu Lys Ala
65 70 75 80
Cys Thr Lys Ile Ser Val Asp Asn Thr Val Val Asn Glu Gln Ala Lys
85 90 95
His Asp Phe Pro Thr Gln Tyr Gln Ile Phe Tyr Pro Leu Asp Asn Gly
100 105 110
Lys Phe Val Ile Ala Asn Lys Gly Thr Gly Trp Val Leu His Ser Gly
115 120 125
Phe Glu Pro Gly Thr Pro Gln Thr Val Val Thr Ser Thr Pro Phe Asp
130 135 140
Ser Asn Asn Ile Ser Asn Gln Ser Phe Glu Arg Lys Ser Lys Asp Ser
145 150 155 160
Ser Gly Asp Phe Arg Leu Ile Asp Lys Asp Gly Trp Ala Val Asn Val
165 170 175
Cys Ser His Ala His Asn Glu Tyr Thr Lys Ile Thr Thr Val Glu Asp
180 185 190
Asn Ala Glu Lys Glu His Leu Leu Leu Lys Phe Lys Thr Ser Glu Asn
195 200 205
Ala Lys Leu Pro Asn Ile Pro Ser Thr Thr Thr Leu Glu Pro Ile Pro
210 215 220
Glu Leu Lys Gly Phe Asn Asp Pro Leu Pro Glu Asn Glu Asp Ala Lys
225 230 235 240
Arg Ala Val Ile Gly Ser Ala Leu Ile Pro Cys Ile Ile Val Asn Asp
245 250 255
His Ile Pro Leu Glu Asp Arg Ile Lys Gln His Pro Tyr Tyr Ile Leu
260 265 270
Glu Tyr Arg Gln Tyr Trp His Leu Leu Trp Ser Asp Val Phe Thr Ala
275 280 285
Gly Ser Asn Lys Ile Lys Thr Glu Val Thr Gly Ile Pro Ser Glu Ala
290 295 300
Gln Glu Lys Met Lys Glu Leu Ile Gly Ala Ser Ile Arg Thr Asp Ile
305 310 315 320
Gly Leu Arg Phe Thr Asp Lys Ser Glu Pro Phe Gly Lys Gln Ile Thr
325 330 335
Ser Gly Leu Asn Val Lys His Ser Tyr Asp Tyr Glu Leu Gly Glu Thr
340 345 350
Thr Thr Thr Thr Asn Val Asp Asn Pro Asn Asp Phe Thr Val Arg Tyr
355 360 365
Ala Arg Tyr Ala Arg Ala His Glu Phe Val Leu Lys Arg Ser Asp Gly
370 375 380
Gln Val Val Glu Glu Pro Trp Val Ala Val Asp Glu Asn Asp Met Tyr
385 390 395 400
Leu Lys Met Tyr Lys Lys Ala
405
<210> 127
<211> 353
<212> PRT
<213> 芽孢杆菌属的种
<400> 127
Met Val Lys Arg Asp Ile Ser Pro Arg Asn Leu Gly Gly Lys Glu Asp
1 5 10 15
Ser Pro Phe Leu Leu Ser Lys Glu Glu Trp Ile Thr Ile Gln Lys Tyr
20 25 30
Thr Gly Asp Gly Ala Tyr Val Pro Val Asn Glu Ala Glu Met Arg Lys
35 40 45
Ala Leu Ala Leu Ser Ser Ser Asp Glu Met Pro Asp Phe Asn Glu Leu
50 55 60
Tyr Ser Val Tyr Thr Asn Val Lys Asn His Cys Gln Asn Trp Thr Asp
65 70 75 80
Asn Thr Tyr Arg Glu Val Leu Gly Ile Ala Asn Glu Ile Val Asn Tyr
85 90 95
Ala Arg Arg Ala Lys Val Tyr Tyr Lys Pro Leu Ile Asp Tyr Leu Pro
100 105 110
Ala Ile Ile Asp Gly Asp Thr Glu Ala Leu Glu Met Phe Lys Lys Ile
115 120 125
Cys Glu Arg Leu Ala Lys Glu Ala Ala Glu Phe Arg Asp His Ala Gly
130 135 140
Ala Leu Ala Thr Met Ile Gly Ile Phe Ala Thr Asp Thr Ala Asn Asp
145 150 155 160
Tyr Gln Ser Leu Ile Val Val Lys Asp Lys Tyr Asp Lys Lys Tyr Gly
165 170 175
Asp His Ser Glu Glu Val Lys Gln Leu Arg Asp Ser Val Asp Arg Leu
180 185 190
Arg Lys Asp Leu Glu Lys Tyr Met Asp Glu Tyr Glu Asp Tyr Gln Ser
195 200 205
Gln Ser Trp Leu Ser Leu Leu Leu Gly Pro Ile Trp Gly Phe Val Leu
210 215 220
Lys Gly Val Leu Asp Ser Thr Lys Gly Lys Ala Leu Lys Ala Arg Ile
225 230 235 240
Asp Ala Thr Lys Lys Gln Ile Glu Glu Ser Gly Lys Ile Ile Gln Arg
245 250 255
Asn Val Tyr Leu Met Ser Leu Leu Asp Lys Thr Asp Thr Gly Thr Asp
260 265 270
Lys Ile Gln Glu Gln Met Ala Ala Ala Leu Pro Val Ile Gln Lys Ile
275 280 285
His Gly Ile Trp Asn Ser Leu His Ser Asp Leu Asp Glu Leu Ser Lys
290 295 300
Ile Val Met Glu Asp Ile Arg Asp Asp Pro Glu Phe Ala Asp Leu Gly
305 310 315 320
Val Glu Leu Ala Ile Met Gln Trp Glu Ala Val Gly Lys Gln Ala Asp
325 330 335
Asp Phe Arg Val Asn Ala Asp Val Gly Phe Val Val Glu Gln Tyr Thr
340 345 350
Ala
<210> 128
<211> 654
<212> PRT
<213> 未知
<220>
<223> 来自环境样品的生物体
<400> 128
Val His Leu Val Phe Leu Ile Leu Ile Tyr Thr Gln Ile Phe Lys Glu
1 5 10 15
Glu Phe Tyr Met Asn Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys
20 25 30
Asp Gly His Asn Val Met Ala Gln Asp Pro Phe Asn Phe Gln His Lys
35 40 45
Ser Leu Asp Thr Ile Gln Lys Glu Trp Thr Glu Trp Lys Arg Asp Asp
50 55 60
His Ser Leu Tyr Val Ala Pro Val Val Gly Thr Val Ala Val Phe Leu
65 70 75 80
Leu Lys Lys Val Gly Ser Phe Ala Ala Lys Arg Ile Leu Asn Gly Leu
85 90 95
Trp Asp Leu Val Phe Pro Asn Asp Asn Thr Lys Leu Met Gln Asp Ile
100 105 110
Leu Arg Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Ala Asp Thr
115 120 125
Leu Ser Arg Val Asn Ala Glu Leu Glu Gly Leu Gln Lys Asn Val Ala
130 135 140
Glu Phe Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Pro
145 150 155 160
Thr Leu Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe
165 170 175
Leu Asn Arg Leu Thr Gln Phe Gln Leu Arg Gly Tyr Glu Leu Leu Leu
180 185 190
Leu Pro Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg
195 200 205
Asp Val Ile Leu Asn Ala Glu Glu Trp Gly Ile Ser Ala Ala Thr Leu
210 215 220
Arg Thr Tyr Arg Asp Tyr Leu Lys Asp Tyr Thr Lys Glu Tyr Ser Asn
225 230 235 240
Tyr Cys Ile Asn Thr Tyr Gln Thr Ala Phe Lys Lys Leu Asn Thr Pro
245 250 255
Leu His Glu Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe
260 265 270
Glu Tyr Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val
275 280 285
Ser Ser Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Lys
290 295 300
Gln Ser Phe Ser Ala Gln Asn Trp Pro Phe Leu Tyr Ser Leu Phe Gln
305 310 315 320
Val Asn Ser Asn Tyr Val Leu Thr Gly Phe Ser Gly Ser Arg Ser Thr
325 330 335
Val Lys Phe Pro Gly Gly Gly Gly Leu Leu Pro Pro Gly Ser Thr Thr
340 345 350
Thr Tyr Ala Leu His Ser Ala Arg Ala Asn Tyr Lys Glu Gly Val Ser
355 360 365
Ser Gly Ile Ile Gly Gly Thr Asn Phe Asn His Asn Phe Lys Cys Arg
370 375 380
Thr Ile Leu Pro Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp
385 390 395 400
Ser Gly Thr Asp Arg Glu Gly Val Thr Thr Phe Thr Asn Trp Gln Thr
405 410 415
Glu Ala Phe Glu Thr Thr Phe Gly Ile Arg Gly Gly Ala Phe Gly Pro
420 425 430
Arg Gly Asn Ala Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser
435 440 445
Gly Leu Pro Leu Val Val Arg Lys Glu Asp Leu Thr Lys Pro Leu His
450 455 460
Tyr Asn Glu Ile Lys Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly
465 470 475 480
Ala Arg Ala Tyr Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr
485 490 495
Asp Ala His Glu Asn Gly Thr Met Ile His Leu Ala Pro Asp Asp Asp
500 505 510
Thr Gly Phe Thr Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln
515 520 525
Ile Arg Thr Phe Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu
530 535 540
Arg Phe Glu Gln Thr Asn Thr Ala Ala Arg Tyr Thr Phe Arg Gly Asn
545 550 555 560
Gly Asn Asn Tyr Thr Leu Tyr Leu Arg Val Ser Ser Gln Gly Asn Ser
565 570 575
Thr Ile Arg Val Asn Ile Asn Gly Arg Val Phe Asn Val Pro Asn Val
580 585 590
Asn Thr Thr Thr Asn Asn Asp Gly Val Ile Asp Asn Gly Ala Arg Phe
595 600 605
Ser Asp Ile Arg Ile Gly Asn Ile Val Ala Ser Asn Asn Thr Asn Ile
610 615 620
Pro Leu Asp Ile Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Glu Leu
625 630 635 640
Met Asn Ile Met Phe Val Pro Asn Asn Leu Pro Pro Leu Tyr
645 650
<210> 129
<211> 648
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 129
Val His Leu Val Phe Leu Ile Leu Ile Tyr Thr Gln Ile Phe Lys Glu
1 5 10 15
Glu Phe Tyr Met Asn Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys
20 25 30
Asp Gly His Asn Val Met Ala Gln Asp Pro Phe Asn Phe Gln His Lys
35 40 45
Ser Leu Asp Thr Ile Gln Lys Glu Trp Thr Glu Trp Lys Arg Asp Asp
50 55 60
His Ser Leu Tyr Val Ala Pro Val Val Gly Thr Val Ala Val Phe Leu
65 70 75 80
Leu Lys Lys Val Gly Ser Phe Ala Ala Lys Arg Ile Leu Asn Gly Leu
85 90 95
Trp Asp Leu Val Phe Pro Asn Asp Asn Thr Lys Leu Met Gln Asp Ile
100 105 110
Leu Arg Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Ala Asp Thr
115 120 125
Leu Ser Arg Val Asn Ala Glu Leu Glu Gly Leu Gln Lys Asn Val Ala
130 135 140
Glu Phe Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Pro
145 150 155 160
Thr Leu Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe
165 170 175
Leu Asn Arg Leu Thr Gln Phe Gln Leu Arg Gly Tyr Glu Leu Leu Leu
180 185 190
Leu Pro Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg
195 200 205
Asp Val Ile Leu Asn Ala Glu Glu Trp Gly Ile Ser Ala Ala Thr Leu
210 215 220
Arg Thr Tyr Arg Asp Tyr Leu Lys Asp Tyr Thr Lys Glu Tyr Ser Asn
225 230 235 240
Tyr Cys Ile Asn Thr Tyr Gln Thr Ala Phe Lys Lys Leu Asn Thr Pro
245 250 255
Leu His Glu Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe
260 265 270
Glu Tyr Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val
275 280 285
Ser Ser Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Lys
290 295 300
Gln Ser Phe Ser Ala Gln Asn Trp Pro Phe Leu Tyr Ser Leu Phe Gln
305 310 315 320
Val Asn Ser Asn Tyr Val Leu Thr Gly Phe Ser Gly Ser Arg Ser Thr
325 330 335
Val Lys Phe Pro Gly Gly Gly Gly Leu Leu Pro Pro Gly Ser Thr Thr
340 345 350
Thr Tyr Ala Leu His Ser Ala Arg Ala Asn Tyr Lys Glu Gly Val Ser
355 360 365
Ser Gly Ile Ile Gly Gly Thr Asn Phe Asn His Asn Phe Lys Cys Arg
370 375 380
Thr Ile Leu Pro Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp
385 390 395 400
Ser Gly Thr Asp Arg Glu Gly Val Thr Thr Phe Thr Asn Trp Gln Thr
405 410 415
Glu Ala Phe Glu Thr Thr Phe Gly Ile Arg Gly Gly Ala Phe Gly Pro
420 425 430
Arg Gly Asn Ala Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser
435 440 445
Gly Leu Pro Leu Val Val Arg Lys Glu Asp Leu Thr Lys Pro Leu His
450 455 460
Tyr Asn Glu Ile Lys Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly
465 470 475 480
Ala Arg Ala Tyr Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr
485 490 495
Asp Ala His Glu Asn Gly Thr Met Ile His Leu Ala Pro Asp Asp Asp
500 505 510
Thr Gly Phe Thr Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln
515 520 525
Ile Arg Thr Phe Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu
530 535 540
Arg Phe Glu Gln Thr Asn Thr Ala Ala Arg Tyr Thr Phe Arg Gly Asn
545 550 555 560
Gly Asn Asn Tyr Thr Leu Tyr Leu Arg Val Ser Ser Gln Gly Asn Ser
565 570 575
Thr Ile Arg Val Asn Ile Asn Gly Arg Val Phe Asn Val Pro Asn Val
580 585 590
Asn Thr Thr Thr Asn Asn Asp Gly Val Ile Asp Asn Gly Ala Arg Phe
595 600 605
Ser Asp Ile Arg Ile Gly Asn Ile Val Ala Ser Asn Asn Thr Asn Ile
610 615 620
Pro Leu Asp Ile Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Glu Leu
625 630 635 640
Met Asn Ile Met Phe Val Pro Asn
645
<210> 130
<211> 394
<212> PRT
<213> 芽孢杆菌属的种
<400> 130
Met Lys Ile Ile Ile Gly Gly Ser Lys Met Lys Lys Met Leu Ile Thr
1 5 10 15
Gly Val Leu Leu Thr Ala Val Ala Thr Asn Tyr Leu Ile Pro Val Asn
20 25 30
Ala Phe Ala Ala Glu Asn His Leu Glu Val Gln Gln Ile Asn Ser Gln
35 40 45
Gln Glu Arg Ala Ser Asp Thr Leu Ser Leu Ser Leu Arg Arg Val Gly
50 55 60
Ser Asn Ser Ala Leu Leu Gln Gly Tyr Gly Leu Val Ile Leu Gln Gln
65 70 75 80
Ser Asn Ile Thr Leu Pro Ala Met Gly Ser Leu Thr Thr His Gln Lys
85 90 95
Ile Ala Lys Thr Asn Val Lys Glu Trp Leu Asp Glu His Asn Pro Lys
100 105 110
Leu Ile Ala Leu Asn Gln Asp Ile Lys Lys Phe Ser Thr Lys Phe Asn
115 120 125
Gly Tyr Tyr Gln Thr Leu Tyr Asp Leu Ala Gly Gln Val Asn Glu Asn
130 135 140
Glu Gln Glu Lys Gln Asn Phe Glu Lys Gly Leu Ser Thr Leu Lys Asp
145 150 155 160
Gln Gly Gln Asn Thr Lys Ser Thr Met Asn Asp Thr Leu Ser Lys Phe
165 170 175
Lys Asp Phe Lys Thr Leu Leu Asp Asp Asp Val Glu Lys Phe Ser Thr
180 185 190
Ala Ala Ser Asp Ala Ile Gln Ser Val Gln Gly Pro Gly Gly Asp Ala
195 200 205
Ile Gln Leu Arg Glu Asn Ile Lys Lys Leu Gln Gln Asp Ile Gln Thr
210 215 220
Glu Leu Thr Asn Ile Leu Asn Arg Pro Ser Glu Ile Ile Asn Gly Ser
225 230 235 240
Ile Asn Phe Gly Lys Lys Val Tyr Thr Ile Gly Met Ser Ser Ala Glu
245 250 255
Ser Gln Thr Ile Asp Leu Val Ser Ile Gln Ser Leu Thr Gly Asp Leu
260 265 270
Leu Asp Leu Ser Asp His Lys Ile Lys Glu Ser Ala Asn Lys Ile Asp
275 280 285
Ala Ile Asn Asn Glu Leu Val Leu Leu Thr Lys Asn Leu Ser Asp Met
290 295 300
Leu Ile Gln Val Thr Gly Ile Thr Phe Ile Glu Asp Gln Val Thr Gly
305 310 315 320
Phe Ala Gly Met Ile Asp Arg Gln Ile Thr Thr Leu Glu Phe Leu Met
325 330 335
Lys Asp Trp Asp Val Ile Asn Glu Lys Ile Asn Gln Ile Gln Ser Asn
340 345 350
Leu Asp Ser Gly Ala Asn Ser Gly Leu Leu Lys Asp Gln Leu Val Glu
355 360 365
Leu Lys Lys Leu Asn Asp Glu Met Glu Asn Gln Ser Lys Gln Phe Glu
370 375 380
Asp Phe Leu Thr Val Glu Ile Thr Gln Ile
385 390
<210> 131
<211> 525
<212> PRT
<213> 芽孢杆菌属的种
<400> 131
Leu Leu Gly Val Met Gly Glu Leu Gln Asn Ile Leu Tyr Lys Arg Ser
1 5 10 15
Gly Lys Met Asn Thr His Gly Lys Asn Glu Glu Trp Asn Gln His His
20 25 30
Asp Gly Thr Cys Gly Cys Gln Gln Asn Lys Gln Asp Tyr Gly Tyr Glu
35 40 45
Gln Ile Pro Glu His Asp Lys Gln Gln Tyr Ala Ser His Glu Tyr Lys
50 55 60
Asn Gln Glu Tyr Thr Tyr Lys Asp Ser Cys Gly Glu Ile Gly Thr Gly
65 70 75 80
Tyr His Asn Glu Asn Asn Gly Asp Phe Gln Cys Asp Thr Ile Arg Tyr
85 90 95
Thr Thr Pro Asn Leu Pro Val Glu Ser Thr Arg Phe Gln Thr Ile Thr
100 105 110
Asn Ile Asn Thr Asn Asp Asn Gly His Arg Val Met Asp Ala Met Asn
115 120 125
Ile Ala Val Gly Thr Gly Leu Glu Gln Arg Val Gly Gly Cys Pro Trp
130 135 140
Gln Ile Asp Val Glu Asn Thr Val Val Asn Glu Gln Val Arg Phe Asn
145 150 155 160
Asn Gln Ser Gln Leu Phe Val Phe Tyr Gln Thr Asp Ser Gly Gln Phe
165 170 175
Val Ile Ala Asn Arg Gly Asn Gly Arg Val Leu Glu Met Val Glu Phe
180 185 190
Pro Asn Ser Asp Pro Gln Trp His Gln Leu Leu Ile Ser Ser Phe Tyr
195 200 205
Glu Asn Arg Arg Asn Gln Leu Phe Asp Arg Arg Ile Ile Asn Asn Thr
210 215 220
Asp Phe Leu Leu Thr Thr Ile Thr Glu Thr Gly Ser Val Phe Gly Val
225 230 235 240
Gln Asn Cys Asn Asn Asn Pro Asn Ala Trp Gln Ile Tyr Thr Thr Val
245 250 255
Asn Glu Asn Glu Ser Val Gly Phe Arg Asn Asn Thr Tyr Arg Phe Arg
260 265 270
Thr Glu Asn Asn Leu Pro Ile Asn Ile Pro Pro Leu Arg Ser Gly Glu
275 280 285
Pro Leu Gly Pro Leu Pro Ala Leu Arg Tyr Leu Gln Asp Ser Gly Leu
290 295 300
Ser Pro Ala Glu Ala Pro Arg Ala Val Ile Gly Ser Ala Leu Leu Pro
305 310 315 320
Ala Ile Phe Val Glu Asp Val Leu Ser Leu Pro Ala Arg Met Ala Gln
325 330 335
Ser Pro Tyr Tyr Val Leu Glu Tyr Arg Gln Tyr Trp His Arg Leu Trp
340 345 350
Ser Asp Ile Ile Pro Ala Asn Gly Gln Ser Trp Phe Asn Glu Thr Thr
355 360 365
Gly Met Leu Gly Gly Ala Pro Gln Gln Gly Asn Met Arg Asn Thr Ile
370 375 380
Ser Met Ser Ile Ala Pro Asp Leu Gly Ile Arg Phe Gly Thr Gln Ser
385 390 395 400
Met Pro Phe Ser Ile Lys Ile Ala Glu Gly Leu Gly Arg Gln Arg Ser
405 410 415
Asn Ala Asn Ile Asp Ile Gly Glu Thr Ser Gln Asn Ile Arg Tyr Asp
420 425 430
Asn Pro Gln Asn Gln Pro Thr Arg Phe Ala Arg Phe Ala Leu Ala His
435 440 445
Glu Tyr Ala Leu Leu Arg Met Asp Gly Arg Val Ile Asn Arg Trp Thr
450 455 460
Val Val Asp Arg Ser Ser Met Tyr Leu Leu Ser Phe Pro Gln Asn Ala
465 470 475 480
Leu Leu Thr Met Gln Cys Asp Lys Ile Ile Arg Thr Asp Asn Ser Tyr
485 490 495
Asp Leu Ser Val Trp Lys Thr Pro Met Gln Ile Lys Ser Gly Gln Val
500 505 510
Ile Thr Lys Asn Glu Lys Asn Ser Lys Pro Tyr Asn Ala
515 520 525
<210> 132
<211> 463
<212> PRT
<213> 芽孢杆菌属的种
<400> 132
Val Arg Ser Gly Ile Asn Met Asp Leu Ser His Ala Asn His Asp Thr
1 5 10 15
Asp Asn Gln Ile Tyr Lys Thr Ser Cys Glu Cys Asn Gln Lys Thr Glu
20 25 30
Trp Pro Met Ser Lys Lys Gln Glu Gln Thr His Thr Asp Thr His Tyr
35 40 45
Lys Ala Thr Gln Phe Asn Ser Pro Ile Trp Asn Leu Pro Ile Glu Ser
50 55 60
Arg Arg Phe Gln Thr Ile Thr Asp Val Asn Ser Ser Gln Gly Arg Gly
65 70 75 80
Leu Asn Ala Leu Arg Thr Tyr Pro Gly Gln Leu Leu Gly Gly Asp Leu
85 90 95
Gly Ala Phe Cys Arg Thr Val Thr Leu Gln Asn Gln Val Val Asn Asp
100 105 110
Leu Thr Arg Gly Asn Ile Ala Arg Phe Leu Phe Tyr Asn Leu Asp Asn
115 120 125
Gly Gly Phe Val Ile Ala Asn Ala Asn Val Gly Glu Val Leu Gln Glu
130 135 140
Met Asn Ala Asn Asn Ser Ser Leu Ile Thr Asn Thr Tyr Thr Gly Gln
145 150 155 160
Thr Asn Gln Phe Phe Arg Lys Ser Pro Ile Thr Gln Asp His Phe Arg
165 170 175
Leu Leu Thr Asn Glu Asn Met Ile Val Gly Pro Cys Gly Asn Asn Ala
180 185 190
Ser Asn Ser Trp Thr Arg Ile Thr Leu Ala Leu Phe Ser Asn Gln Asn
195 200 205
Phe Arg Tyr Arg His Glu Pro Ser Glu Asn Gln Pro Ile Asn Leu Pro
210 215 220
Gln Ala Ala Ala Thr Ser Glu Leu Ala Pro Leu Pro Ala Leu Thr Ser
225 230 235 240
Leu Asn Asp Pro Gly Pro Ser Pro Val Val Ala Pro Arg Val Thr Ile
245 250 255
Gly Ser Ala Leu Ile Pro Cys Ile Phe Val Asn Asp Val Ile Pro Leu
260 265 270
Gln His Arg Ile Arg Gln Ser Pro Tyr Tyr Val Leu Glu Cys Arg Gln
275 280 285
Tyr Trp His Arg Ile Trp Ser Asp Val Leu Thr Ala Gly Ser Asp Ala
290 295 300
Phe Tyr Glu Glu Arg Thr Gly Ile Ser Asp Gly Ala Gln Ile Asp Ile
305 310 315 320
Arg Asn Thr Met Asp Ile Ser Ile Gly Ser Asp Trp Tyr Leu Arg Phe
325 330 335
Gly Ser Leu Ser Ser Pro Phe Arg Gly Gln Ile Ser Ser Gly Leu Arg
340 345 350
Thr Gln Pro Ser Phe Ser Asn Ile Asp Leu Ala Glu Thr Val Met Ile
355 360 365
Asn Arg His Ile Asn Pro Asn Ser Phe Thr Thr Arg Phe Ser Arg Phe
370 375 380
Thr Lys Ala Ile Glu Tyr Arg Leu Leu Arg Met Asp Gly Thr Val Val
385 390 395 400
Arg Ser Trp Val Ala Val Asp Asn Arg Ser Met Tyr Trp Lys Thr Phe
405 410 415
Pro Asn Asn Ala Gln Ser Thr Met Gln Ser Gln Lys Ile Ile Gln Ser
420 425 430
Asp Thr Ser Tyr Asp Leu Ser Val Trp Lys Thr Pro Met Val Ile Arg
435 440 445
Asp Gly Glu Ile Ile Glu Lys Asn Asp His Asn Ser Lys Thr Gln
450 455 460
<210> 133
<211> 952
<212> PRT
<213> 芽孢杆菌属的种
<400> 133
Met Ile Met Gln Met Val Thr Lys Ala Met Thr Pro Lys Leu Gly Ala
1 5 10 15
Leu Pro Asp Phe Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Asp
20 25 30
Asn Ile Thr Gly Val Met Ser Thr Ile Phe Gly Val Asp Thr Gly Asp
35 40 45
Ser Ser Ile Asp Asn Val Leu Asn Asn Gln Glu Leu Leu Gln Glu Met
50 55 60
Leu Asp Gln Met Ser Thr Met Gln Ser Ser Ile Gln Asp Ile Leu Glu
65 70 75 80
Gln Gln Gly Ile Ser Ala Asp Ile Glu Lys Gln Ile Leu Ala Leu Thr
85 90 95
Thr Asp Leu Ala Thr Ser Ile Asn Thr Glu Leu Gly Lys Ile Glu Gly
100 105 110
Ile Leu Asn Thr Tyr Leu Pro Ala Ile Ser Asn Met Leu Ser Asn Ile
115 120 125
Tyr Glu Gln Thr Ser Leu Ile Asp Gln Lys Val Asp Lys Leu Leu Ala
130 135 140
Leu Met Thr Phe Ala Leu Lys Glu Leu Asp Tyr Ile Lys Asp Asn Val
145 150 155 160
Val Leu Asn Ser Ser Ile Val Glu Ile Thr Pro His Val Gln Lys Leu
165 170 175
Val Tyr Val Asn Lys Lys Phe Leu Ser Leu Thr Arg Gly Phe Leu Gln
180 185 190
Gly Glu Asp Val Ser Ile Asp Ser Met Gln Glu Ile Gln Glu Trp Ala
195 200 205
Lys Ser Ile Leu Ala Thr Glu Met Asn Ser Phe Glu Phe Ser Val Asp
210 215 220
Thr Leu His Asn Ile Ile Ile Gly Asp Asn Leu Tyr Lys Arg Ser Ala
225 230 235 240
Leu Lys Thr Phe Ser Asp Val Leu Leu Asp Asp Ala Asp Gln Tyr Gly
245 250 255
Asp Phe Gly Thr Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser Ser Leu
260 265 270
Ala Thr Leu Gln Ile Asn Ala Tyr Leu Cys Leu Thr Phe Ala Arg Lys
275 280 285
Val Leu Gly Leu Ser Glu Ile Asp Tyr Gln Val Thr Met Gln Asp Arg
290 295 300
Ile Glu Gln Gln Asn Gln Met Phe Val Asn Leu Ile Gln Asp Lys Asn
305 310 315 320
Tyr Ser Asn Ala Leu Glu Ile Thr Gly Ile Tyr Pro Met Ser Val Asp
325 330 335
Trp Gly Asp Cys Lys Ser Thr Asp Leu Gln Ala Asp Ala Gly Tyr Ala
340 345 350
Leu Ile Gly Leu Glu Phe Phe Met Asp Asn Gly Ile Tyr Lys Val Lys
355 360 365
Ala Tyr Gln Gly Lys Ile Gly Lys Asn Phe Ser Val Ser Gly Asp Thr
370 375 380
Val Thr Glu Ile Ile Ser Asp Asp Leu Ser Thr Val Phe Asn Asp Met
385 390 395 400
Thr Asn Asp Asn Pro Glu Leu Asp Val Val Tyr Pro Leu Ser Gly Glu
405 410 415
Leu Thr Gly Pro Pro Asn Thr Ile Ile Thr Arg Ile Gly Phe Gly Thr
420 425 430
Lys Tyr Asp Lys Thr Arg Glu Ser Ser Val Gln Ala Phe Ala Tyr Ile
435 440 445
Asp Ala Asp Phe Ser Pro Tyr Asp Tyr Ile Ser Gly Thr Ile Ser Lys
450 455 460
Glu Gly Thr Gln Thr Val Ser Leu Glu Gly Asn Asp His Lys Asn Arg
465 470 475 480
Cys Tyr Ser Met Trp Pro Ile Gly Leu Ile Gly Asp Leu Tyr Met Thr
485 490 495
Pro Leu Lys Ser Leu Ser Leu Asn Val Asp Val Asp Asp Ser Ser Ala
500 505 510
Tyr Gly Thr Ile Leu Asn Met Ser Gly Glu Ser Tyr Phe Ser Thr Ile
515 520 525
Leu Ser Arg Glu Tyr Asn Thr Asn Phe Ile Leu Phe Pro Tyr Thr Asn
530 535 540
Asn Ser Gly Leu Ile Asp Glu Asn Leu Ile Gln Asn Gly Asn Leu Glu
545 550 555 560
Glu Gln Asp Lys Tyr Trp Glu Gly Thr Glu Pro Ser Phe Phe Ala Glu
565 570 575
Gly Glu Gly Thr Phe Gly Ser Asn Ala Leu Lys Val Gln Ala Gln Gly
580 585 590
Ala Phe Asp Gln Ile Val Lys Leu Glu Pro Asn Thr Thr Tyr Arg Leu
595 600 605
Thr Ala Phe Thr Lys Val Lys Asp Ser Asn Tyr Ser Lys Gly Arg Ile
610 615 620
Gly Ile Arg Asp Met Tyr Asn Tyr Arg Val Glu Lys Ser Phe Leu His
625 630 635 640
Thr Arg Tyr Lys Gln Thr Lys Val Glu Phe Thr Thr Gly Ala Asp Thr
645 650 655
Ser Lys Leu Ser Val Phe Ile Gln Gly Gly Gly Asp Gln Glu Ser Ile
660 665 670
Ala Trp Ala Asp Asn Ile Glu Leu Tyr Lys Val His Gln Gln Asp Asn
675 680 685
Met Ile Ser Asp Phe Asp Phe Asp Thr Tyr Lys Tyr Ser Asn Met Cys
690 695 700
Trp Lys Leu Ser Gly Gly Gly Asp Phe Val Glu Lys Glu Gly Leu Phe
705 710 715 720
Asn Ser Lys Ala Leu Lys Ile Thr Asn Ser Gly Gly Ala Glu Gln Glu
725 730 735
Val Lys Leu Lys Ala Asn Thr Asn Tyr Ile Leu Thr Ala Tyr Val Lys
740 745 750
Val Asp Val Pro Thr Thr Thr Ala Gln Ile Gly Cys Gly Ser Asn Gln
755 760 765
Val Pro Cys Asn Ser Thr Ser Tyr Thr Pro Val Lys Leu Lys Phe Lys
770 775 780
Thr Gly Glu Asp Pro Ser Thr Thr Glu Ser Ser Val Tyr Cys Ser Asn
785 790 795 800
Ser Asn Asp Ser Gly Thr Val Trp Ala Asp Asn Phe Val Leu Tyr Glu
805 810 815
Ala Pro Asn Leu Ile Ile Asn Gly Asp Phe Glu Gln Phe Asp Gln Ser
820 825 830
Ser Trp Asn Phe Ser Pro Ser Glu Gly Gly Arg Ile Tyr Leu Gln Ser
835 840 845
Gly Leu Gly Met Ser Arg Ser Asn Ala Val Val Leu His Gly Gln Asn
850 855 860
Gly Gln Ile Ser Gln Lys Val Pro Leu Lys Pro Phe Thr Lys Tyr Arg
865 870 875 880
Leu Thr Ala Tyr Val Arg Val Ser Asp Gly Gly Thr Ala Trp Ile Gly
885 890 895
Tyr Gly Asp Tyr Gly Ala Ser Cys Thr Ser Lys Glu Phe Lys Gln Ile
900 905 910
Ser Lys Asp Phe Thr Thr Gly Ala Asn Pro Leu Gln Ser Asp Asp Ala
915 920 925
Ile Tyr Leu Ser Ser Asp Asn Ser Lys Tyr Thr Val Ala Asp Asn Phe
930 935 940
Glu Leu Tyr Glu Leu Asp Gln Ile
945 950
<210> 134
<211> 354
<212> PRT
<213> 未知
<220>
<223> 来自环境样品的生物体
<400> 134
Met Glu Ile Lys Lys Asp Ala Tyr Tyr Lys Ile Val Asn Arg Asn Ser
1 5 10 15
Gly Leu Val Ala Asp Val Ala Gly Lys Ser Lys Gly Asn Ser Ala His
20 25 30
Leu Glu Gln Leu Asp Trp His Gly Glu Pro Phe Gln Gln Trp Leu Leu
35 40 45
Tyr Pro Leu Asp Gly Gly Arg Tyr Ala Ile Ile Asn Arg Asn Ser Gly
50 55 60
Leu Val Ala Asp Val Ala Gly Lys Ser Thr Ser Asn Ser Ala Arg Leu
65 70 75 80
Glu Gln Leu Asp Trp His Gly Glu Pro Phe Gln Gln Trp Leu Leu Tyr
85 90 95
Gln Thr Asn Asp Gln Gly Tyr Tyr Lys Ile Val Asn Gln Asn Ser Gly
100 105 110
Leu Val Ala Asp Val Ala Gly Lys Ser Lys Gly Asn Ser Ala His Leu
115 120 125
Glu Gln Leu Asp Asp Gln Asn Glu Ser Phe Gln Tyr Trp Ser Phe Asp
130 135 140
Glu Val Glu Ser Ile Thr Leu Pro Ser Val Glu Thr Lys Pro Leu Pro
145 150 155 160
Ile Ala Pro Gln Tyr Lys Ser Leu Asn Asp Tyr Leu Pro Asp Ser Thr
165 170 175
Glu Pro Val Val Thr Ala Tyr Thr Leu Leu Pro Cys Ile Met Val Asn
180 185 190
Asp Asn Asn Trp Ser Ala Asp Lys Lys Met Gln His Ser Pro Tyr Tyr
195 200 205
Met Leu Thr Lys Thr Gln Tyr Trp Lys Lys Ile Asp Ser His Thr Phe
210 215 220
Ala Pro His Ala Gln Tyr Thr Ser Glu Thr Lys Tyr Gly Met Ser Gln
225 230 235 240
Thr Asp Gln Glu Ser Met Thr Arg Asn Thr Asn Ile Ser Val Lys Ala
245 250 255
Asp Ala Gly Phe Ser Phe Lys Ala Val Ser Ile Gly Ile Ser Lys Ser
260 265 270
Val Thr Lys Glu Leu Gln Val Ser Lys Ser Thr Thr Thr Thr Gln Met
275 280 285
Thr Glu Gln Thr Asn Ser Glu Thr Ile Ala Asn Pro Asn Asn Tyr Thr
290 295 300
Val Ala Trp Thr Lys Tyr Ala Leu Val Thr Glu Tyr Thr Leu Lys Arg
305 310 315 320
Gly Asp Gly Ile Arg Val Ser Asp Pro Trp Thr Val Ile Asn Lys Asn
325 330 335
Asp Glu Arg Glu Ser Tyr Trp Pro Leu Asp Ala Pro Leu Asn Lys Ala
340 345 350
Gln Ile
<210> 135
<211> 375
<212> PRT
<213> 芽孢杆菌属的种
<400> 135
Met Met Lys Lys Ile Pro Tyr Lys Ile Ile Val Ala Ser Ala Leu Leu
1 5 10 15
Thr Met Thr Thr Thr Ser Val Val Ser Pro Val Ala Ala Phe Ala Ser
20 25 30
Glu Ile Glu Gln Pro Ser Thr Gly Asp Met Ser Leu Ser Ala Asn Glu
35 40 45
Val Lys Met Lys Glu Ala Leu Gln Lys Ala Gly Leu Phe Ala Lys Ser
50 55 60
Met Asn Glu Tyr Ser Tyr Leu Leu Ile Asn Asn Pro Asp Val Asn Phe
65 70 75 80
Glu Gly Ile Thr Ile Asn Gly Tyr Ala Asp Leu Pro Asn Lys Ile Val
85 90 95
Gln Asp Gln Arg Asn Ala Arg Ala His Ala Val Thr Trp Asn Thr Gln
100 105 110
Val Lys Arg Gln Leu Leu Asp Thr Leu Thr Gly Ile Val Glu Tyr Asp
115 120 125
Thr Met Phe Asp Asn Tyr Tyr Asp Thr Ile Ile Asp Ala Ile Asn Thr
130 135 140
Gly Asp Gly Asp Thr Leu Lys Glu Gly Ile Thr Asp Leu Arg Ser Glu
145 150 155 160
Ile Gln Gln Asn Gln Lys Thr Ala Gln Asn Leu Ile Ala Glu Leu Thr
165 170 175
Lys Leu Arg Asp Asn Met Gly Glu Asp Val Arg Ala Phe Gly Ser Asn
180 185 190
Lys Glu Gln Leu Tyr Ser Ile Leu Lys Asn Gln Thr Ala Gly Leu Glu
195 200 205
Asp Asp Gln Lys Arg Leu Asp Glu Val Leu Gly Gln Val Asn Tyr Tyr
210 215 220
Lys Lys Leu Glu Ser Asp Gly Phe Thr Thr Met Lys Val Gly Val Phe
225 230 235 240
Gly Leu Trp Trp Ile Gly Gly Ile Ile Val Gly Thr Ala Arg Asp Asn
245 250 255
Leu Gly Lys Leu Glu Pro Tyr Leu Ala Glu Val Arg Gln Thr Val Asp
260 265 270
Asn Lys Thr Thr Leu Asn Arg Val Ile Glu Val Ala His Asn Asn Thr
275 280 285
Asp Gln Met His Lys Ser Ile Asp Asn Ala Ile Asn Ala Leu Glu Tyr
290 295 300
Met His Thr Gln Trp Thr Asp Leu Asp Asn Gln Tyr Ala Gly Val Leu
305 310 315 320
Gln Lys Ile Asp Asn Ala Ala Gly Lys Ala Asp Gln Asn Lys Phe Ala
325 330 335
Tyr Leu Lys Pro Thr Leu Lys Thr Ala Lys Asp Leu Trp Lys Arg Leu
340 345 350
Asn Glu Asp Ala Val Ile Ile Lys Glu Gly Ile Lys Glu Leu Lys Val
355 360 365
Glu Pro Val Thr Pro Gln Lys
370 375
<210> 136
<211> 353
<212> PRT
<213> 芽孢杆菌属的种
<400> 136
Met Val Lys Arg Asp Ile Ser Ala Arg Asp Leu Gly Gly Lys Glu Asp
1 5 10 15
Thr Pro Phe Leu Leu Lys Lys Asp Glu Trp Ile Lys Ile Gln Lys Tyr
20 25 30
Thr Gly Asp Gly Ala Tyr Leu Pro Val Asn Val Asn Glu Met Arg Lys
35 40 45
Ala Leu Ala Leu Glu Asn Thr Ala Glu Leu Pro Asp Phe Gly Glu Leu
50 55 60
Tyr Thr Val Asn Ser Ser Ile Lys Gly His Cys Lys Asn Trp Asn Asp
65 70 75 80
Thr Thr Tyr Arg Glu Ile Leu Asp Val Ala Ser Gln Ile Val Asn Tyr
85 90 95
Ser Arg Arg Ala Glu Val Phe Tyr Ala Pro Leu Leu Val Tyr Leu Pro
100 105 110
Asp Ile Leu Glu Gly Asp Thr Val Ala Leu Glu Lys Phe Gln Lys Ile
115 120 125
Cys Lys Lys Leu Ala Thr Glu Ala Lys Asp Phe Ser Asp His Ala Glu
130 135 140
Thr Leu Ala Lys Thr Val Glu Gln Phe Ala Asn Asp Thr Ala Thr Asp
145 150 155 160
Tyr Lys Asn Leu Ile Asn Val Lys Asp Lys Tyr Asp Ala Leu Tyr Gly
165 170 175
Lys Asn Ser Glu Glu Glu Ile Arg Leu Arg Ala Glu Val Glu Lys Leu
180 185 190
Arg Glu Glu Leu Glu Glu Tyr Ile Glu Asp Leu Glu Asp Phe Glu Glu
195 200 205
Glu Val Trp Leu Ser Leu Leu Leu Gly Pro Ile Phe Gly Phe Val Leu
210 215 220
Lys Glu Ile Leu Glu Ser Thr Lys Gly Lys Met Leu Gln Ala Lys Ile
225 230 235 240
Asp Ala Thr Arg Gln Lys Ile Glu Ala Thr Cys Ala Thr Ile Gln Arg
245 250 255
Asn Val Tyr Leu Met Ser Leu Leu Asp Lys Ala Asp Val Gly Thr Asp
260 265 270
Lys Thr Gln Lys Gln Ile Glu Glu Ala Leu Pro Val Ile Asn Lys Ile
275 280 285
Lys Ala Ile Trp Tyr Ser Leu His Thr Asp Leu Leu Thr Leu Ser Asn
290 295 300
Ile Thr Met Lys Asp Ile His Asp Asp Pro Glu Phe Ala Asp Leu Gly
305 310 315 320
Ile Glu Leu Ala Ile Met Gln Trp Val Lys Val Gly Lys Gln Ala Asp
325 330 335
Asp Phe Arg Ile Asn Ala Asp Val Gly Phe Ile Val Asp Gln Tyr Ile
340 345 350
Ala
<210> 137
<211> 284
<212> PRT
<213> 芽孢杆菌属的种
<400> 137
Met Phe Asp Phe Asp Pro His Ile Ile Thr Tyr Ser Tyr Leu Glu Arg
1 5 10 15
Met Ile Val Gly Met Val Thr Lys Val Met Thr Pro Ser Leu Lys Ser
20 25 30
Leu Pro Asp Phe Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Gly
35 40 45
Asn Ile Ser Asp Ile Met Ser Thr Ile Phe Gly Ile Asp Val Gly Gly
50 55 60
Asn Ser Asp Ile Asp Asp Val Leu Asn Asn Ala Glu Leu Leu Gln Gln
65 70 75 80
Met Met Asp Gln Met Asn Ser Met Gln Gly Ser Leu Ala Asp Ile Leu
85 90 95
Glu Glu Gln Gly Met Ser Ile Glu Leu Ile Gln Lys Leu Thr Ser Leu
100 105 110
Ser Asn Gln Gln Leu Ala Leu Ser Thr Pro Ile Asn Thr Glu Leu Gly
115 120 125
Lys Ile Glu Asp Ile Leu Asn Asn Tyr Leu Pro Ala Ile Ser Asn Met
130 135 140
Leu Asn Glu Val Tyr His Glu Thr Val Gln Ile Asn Ala Lys Val Asp
145 150 155 160
Lys Leu Leu Ser Leu Met Met Tyr Ala Met Lys Glu Leu Asp Leu Ile
165 170 175
Lys Asp Asn Val Ile Leu Asn Ser Ser Val Val Glu Ile Thr Pro His
180 185 190
Val Gln Lys Leu Val Tyr Val Asn Lys Lys Phe Leu Glu Leu Ser Gly
195 200 205
Asn Tyr Met Arg Ser Asp Ile Ser Ile Asp Ser Met Gln Glu Leu Leu
210 215 220
Gln Trp Ala Lys Ser Ile Val Asp Thr Asp Met Asn Ser Phe Val Ser
225 230 235 240
Thr Asp Thr Pro Gly Asn Lys Val Phe Cys Ser Ile His Arg Ala Val
245 250 255
Val Lys Ser Asn Asn Ile Phe Gly Arg Ser Leu Cys Asn Gln Ala Arg
260 265 270
Val Tyr Asn His Leu Ile Arg Arg Gln Cys Pro Gly
275 280
<210> 138
<211> 372
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 138
Met Pro Glu Asn Asn Arg Tyr Tyr Asn Ile Gln Leu Lys Ser Asn Pro
1 5 10 15
Lys Lys Val Trp Asn Val Ser Gln Ala Ser Asn Asp Asn Asp Arg Ala
20 25 30
Ile Leu Leu Trp Gln Gly Gly Ser Ala Asp His Glu Lys Phe Ala Phe
35 40 45
Phe Gln Leu Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly
50 55 60
Lys Val Val Thr Phe Gly Thr Gly Ser Trp Ile Ser Gly Gly Pro Ile
65 70 75 80
Pro Ala Ser Glu Thr Leu Gln Gln Gln Ser Trp Thr Gly Glu Ala Lys
85 90 95
Gln Lys Trp Asn Leu Arg Asp Leu Gly Ser Asn Gly Asp Arg Ser Asn
100 105 110
Asn Tyr Glu Ile Val Asn Gln Ala Asn Gly Lys Val Pro Ser Tyr Arg
115 120 125
Trp Ile Gly Ser Thr Val Asp Asn Thr Glu Phe Leu Gln Leu Asp Asn
130 135 140
Ser Asn Pro Ser Asp Ala Asp Lys Val Phe Thr Leu Phe Asn Gly Thr
145 150 155 160
Gly Asn Leu Phe Gly Ile Asp Ile Thr Phe Phe Asn Asp Thr Ile Ser
165 170 175
Val Pro Lys Leu Pro Ala Ile Gly Thr Arg Pro Asp Val Pro Asp Tyr
180 185 190
Thr Gly Gly Val Asn Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val
195 200 205
Val Gly Ala Ser Leu Ile Pro Tyr Phe Met Val Lys Asp Asn Gln Ala
210 215 220
Asn Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys
225 230 235 240
Glu Glu Tyr Trp Asp Lys Thr Phe Ser Lys Val Ile Gln Pro Gly Leu
245 250 255
Ser Glu Ala Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln
260 265 270
Lys Met Thr Asp Thr Leu Ser Met Asn Phe Gly Ala Asp Leu Gly Ile
275 280 285
Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr
290 295 300
Ile Gln Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr
305 310 315 320
Arg Ser Tyr Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr
325 330 335
Gln Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser
340 345 350
Ile Val Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr
355 360 365
Arg Lys Thr Ser
370
<210> 139
<211> 373
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 139
Met Asp Arg Ile Asn Ile Asp Gly Val Arg Thr Gly Gly Pro Val Ser
1 5 10 15
Asp Gly Gly Asn Phe Ser Asp Thr Ser Gly Gly Gln Thr Phe Ser Asp
20 25 30
Lys Glu Leu Ala Asn Ile Tyr Leu Gln His Trp Lys Glu Gly Lys Tyr
35 40 45
Gly Asn Trp Arg Asp Gly Leu Tyr Met Ser His Gly Asp Phe Ser Asp
50 55 60
Phe Pro Pro Glu Phe Lys Ala Gly Val Met Ser Pro Gly Tyr Leu Ile
65 70 75 80
Pro Gln Ala Gln Leu Pro Ala Ile Ile Glu Lys Ile Lys Asn Thr Glu
85 90 95
Gln Leu Glu Leu Met Asp Leu Leu Gln Ile Leu Thr Pro Glu Thr Thr
100 105 110
Val Asp Lys Asp Val Gln Lys His Arg Tyr Leu Thr Val Asn Thr Asp
115 120 125
Lys Lys Thr Arg Ile Asn Ile Asp Lys Thr Glu Val Ile Ser Ala Pro
130 135 140
Ile Ala Ala Pro Gln Lys Val Thr Asn Asp Thr Asp Gln Glu Gly Ser
145 150 155 160
Ala Thr Ala Pro Ser Ile Ser Lys Lys Phe Thr Ser Thr Ile Ser Arg
165 170 175
Thr Asp Thr His Ser Thr Thr Leu Gly Met Lys Gln Thr Ile Lys Gly
180 185 190
Gly Ile Asp Phe Leu Gly Ile Ala Glu Gly Asp Phe Ser Gln Glu Phe
195 200 205
Asn Glu Ser Tyr Thr Tyr Ser Asn Ala Ser Gln Lys Leu Thr Ser Asp
210 215 220
Glu Thr Thr Ile Thr Ser Gly Thr Val Thr Ile Lys Thr Gln Pro His
225 230 235 240
Ser Ala Tyr Lys Phe Asp Ile Ile Phe Gln Gln Val Lys Asn Ser Gly
245 250 255
Thr Val Thr Gly Thr Ser His Leu Gly Gly Gly Tyr Gly Ser Ser Gly
260 265 270
Lys Asp Ala Tyr Tyr Asp Leu Ser Ile Tyr Lys Lys Met Lys Val Ile
275 280 285
Gln Asp Leu Tyr Pro Gln Leu Trp Ala Val Leu Gln Ala Lys Gly Ile
290 295 300
Asp Val Asn Asp Thr Thr Gln Glu Val Asn Tyr Thr Gly Ala Ile Ser
305 310 315 320
Phe Glu Gly Ile Thr Gly Ala Lys Ile Asp Val Thr Val Lys Asn Leu
325 330 335
Ala Thr Gly Asp Val Ile Asn Arg Leu Ile Thr Pro Thr Pro Val Lys
340 345 350
Pro Gly Thr Asn Met Asn Ile Thr Glu Gln Leu Gln Gly Thr Ile Gln
355 360 365
Ser Ile Val Ser Gln
370
<210> 140
<211> 1133
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 140
Met Asn Pro Asn Glu Glu Tyr Lys Thr Thr Val Pro His Ser Asn Lys
1 5 10 15
Ile Gln Pro Arg Tyr Pro Leu Thr Asn Asn Pro Asp His Pro Leu Gln
20 25 30
His Thr Asn Tyr Lys Asp Trp Leu Gln Met Cys Gln Asn Thr Ser Phe
35 40 45
Asp Gly Glu Asn Pro Val Pro Ser Val Thr Glu Val Gly Ala Ile Gly
50 55 60
Ile Ala Ile Ser Ile Val Ser Thr Leu Leu Ser Thr Phe Gly Ala Ala
65 70 75 80
Val Pro Gly Ala Ala Leu Ala Val Leu Gly Ala Thr Leu Pro Phe Phe
85 90 95
Asp Ser Gly Ser Gly Ser Val Trp Glu Asp Phe Met Ile His Asn Ser
100 105 110
Ser Val Leu Gln Lys Thr Leu Pro Glu Ser Ile Lys Asn Gln Ala Asn
115 120 125
Thr Ile Leu Thr Gly Leu Gln Glu Ser Leu Ile Glu Tyr Lys Arg Val
130 135 140
Phe Asn Ile Trp Lys Lys Asp Lys Thr Pro Ala His Ala Glu Gln Val
145 150 155 160
Arg Ser Thr Tyr Arg Asn Thr His Asn Leu Phe Ile Asn Arg Met Ser
165 170 175
Glu Leu Arg Pro Ser Gln Tyr Lys Gln Ile Leu Leu Ser Val Tyr Cys
180 185 190
His Ala Ala Asn Leu His Leu Gly Leu Leu Val Gln Gly Val Ala Tyr
195 200 205
Tyr Asn Glu Trp Tyr Asn Val Asn Pro Ser Leu Leu Thr Pro Asp Arg
210 215 220
Thr Val Asp Asp Glu Tyr Leu Leu Leu Gln Asp Lys Ile Lys Glu Tyr
225 230 235 240
Ile Asp Tyr Cys Ser Lys Thr Tyr Arg Glu Gly Leu Asn Ile Leu Ala
245 250 255
Asn Ser Gln Ala Asn Leu Lys Ile Tyr Thr Thr Tyr Gln Arg Glu Met
260 265 270
Thr Phe Thr Val Leu Asp Ile Ile Thr Asn Phe Pro Tyr Tyr Asp Phe
275 280 285
Lys Lys Tyr Pro Ile Gly Val Gln Ser Glu Leu Thr Arg Glu Ile Tyr
290 295 300
Thr Ile Glu Gln Gln Ile Lys Gln Ile Tyr Leu Asn His Leu Thr Arg
305 310 315 320
Gln Pro Asn Leu Val Thr His Pro Phe Asn Ser Ser Ile Tyr His Tyr
325 330 335
Arg Asn Leu Pro Phe Ile Thr Ser Gly Phe Thr Ala Val Arg Asn Arg
340 345 350
Phe Trp Phe Thr Asn Ala Arg Asn Leu Ser Val Ser Gly Leu Tyr Gly
355 360 365
Ser Thr Ser Phe Asp Gly Gln Phe Asp Val Ser Thr Gln Asn Val Trp
370 375 380
Lys Val Glu Ala Thr Tyr Ala Asp Val Ala Arg Arg Leu Lys Gln Leu
385 390 395 400
Lys Phe Tyr Ala Gln Leu Pro Asn Pro Ile Thr Ala Thr Thr Gly Thr
405 410 415
Pro Asn Asn Ser Asp Tyr Arg Glu Leu Phe Thr His Glu Ile Pro Gly
420 425 430
Arg Asp Gly Lys Leu Pro Thr Thr Phe Gln Thr Ala Ser His Tyr Leu
435 440 445
Ser Tyr Met Met Met Thr Ala Ser Glu Ser Pro Gly Leu Ser Cys Ala
450 455 460
Trp Arg His Ala Ser Val Asn Asn Asp Asn Leu Ile Ser Pro Asn Lys
465 470 475 480
Ile Thr Leu Ile Pro Ala Val Lys Ala Arg Glu Leu Asp Thr Ser Ser
485 490 495
Lys Val Val Ser Gly Pro Gly His Thr Gly Gly Asp Leu Val Glu Phe
500 505 510
Asn Met Ala His Gly Arg Leu Glu Leu Gln Gly Ser Ile Gly Asn Val
515 520 525
Ser Gln Glu Arg Phe Thr Ile Arg Leu Arg Tyr Ala Ala Ala Ala Asn
530 535 540
Glu Ser Pro Arg His Leu Leu Val Thr Ile Pro Asn Ile Gly Val Phe
545 550 555 560
Pro Val Met Leu Leu Pro Thr Thr Tyr Lys Leu Ser Leu Asn Glu Phe
565 570 575
Ser Tyr Asn Glu Phe Gly Phe Val Asp Ile Ile Pro Ser Asn Glu Ser
580 585 590
Ile Ser Phe Phe Ala Ser Ile Pro Trp Ile Val Ser Ile Thr Ser Thr
595 600 605
Arg Gly Ser Ile Asp Asp Lys Val Tyr Ile Asp Arg Leu Glu Phe Ile
610 615 620
Pro Ile Ser Arg Leu Pro Asn Arg Gln Arg Gln Gln Leu Glu Lys Thr
625 630 635 640
Gln Gln Val Val Asp Gly Leu Phe Thr Ser Ser Glu Lys Gln Glu Ile
645 650 655
Lys Met Ser Thr Thr Asp Phe Arg Ile Asp Gln Thr Ser Thr Leu Val
660 665 670
Glu Cys Leu Ser Asp Thr Glu Tyr Pro Gln Glu Gln Leu Leu Leu Leu
675 680 685
Asp Gln Val Lys Tyr Ala Lys Gln Leu Ser Gln Ser Arg Asn Leu Leu
690 695 700
Ser Asn Thr Phe Gly Asn Gln Gly Trp Ser Thr Gln Gly Asp Val Val
705 710 715 720
Leu Ser Ser Gly Gly Ser Leu Phe Lys Asp Ser Tyr Phe Ser Leu Pro
725 730 735
Gly Ala Arg Thr Thr Gln Val Gly Pro Asp Thr Phe Pro Thr Tyr Met
740 745 750
Tyr Gln Lys Ile Asp Glu Ser Gln Leu Lys Pro Tyr Thr Arg Tyr Lys
755 760 765
Val Arg Gly Phe Ile Glu Ala Ser Asn His Leu Glu Val Ala Ile Ser
770 775 780
Arg Tyr Gly Asn Glu Val Lys Thr Met Leu His Val Pro Asp Asp Leu
785 790 795 800
Ala Thr Ser Ser Thr Arg Asp Asn Cys Gly Glu Trp Asn Arg Cys Glu
805 810 815
Thr Thr Gln His Phe Phe Thr Glu Asn Thr Glu Glu Ile Ser Lys Pro
820 825 830
Ala Val Ser Ser Thr Thr Cys Ser Pro Cys Asp Ala Thr His Thr Ser
835 840 845
His Lys Thr Cys Ile Asp Ser His Asp Phe Ser Phe Thr Ile His Thr
850 855 860
Gly Asp Ile Asp Ile Thr Asn Lys Pro Gly Ile His Ile Leu Phe Lys
865 870 875 880
Ile Ser Asp Pro Asp Gly Phe Ala Thr Leu Gly Asn Leu Glu Val Ile
885 890 895
Glu Asp Gly Pro Ile Phe Glu Asp Thr Ile Ser Thr Val Lys Tyr Gln
900 905 910
Glu Gln Arg Trp Lys Gln Asn Leu Ile Glu Gln Gln Gln Lys Thr Glu
915 920 925
Gln Ala Phe Leu Gln Val Gln Gln Ala Val Asp Ser Leu Phe Gln Asp
930 935 940
Ser Gln Tyr Gln Thr Leu Gln Leu Glu Val Thr Ala Ser Gln Ile His
945 950 955 960
Ser Ala Glu Asn Leu Leu Gln Glu Ile Ser Ser Met His Asn Asp Ile
965 970 975
Gln Ser Pro Lys Asn Glu Glu Arg Phe Thr Ala Leu Asn Asn Gln Ile
980 985 990
Gln Gln Ala Tyr His Leu Tyr Ser Lys Arg Asn Met Val Arg Asn Gly
995 1000 1005
Asp Phe Ser Glu Gly Leu His Tyr Trp Ser Ala Ser Ser Asp Ala
1010 1015 1020
Ser Ile Gln Gln Thr Gln Asp Asp Ser Val Leu Leu Ile Pro Ser
1025 1030 1035
Trp Ser Thr Gln Val Ser Gln Gln Leu Arg Leu Gln Pro Asn His
1040 1045 1050
Arg Tyr Leu Leu Arg Val Ala Ala Leu Lys Glu His Ile Gly Lys
1055 1060 1065
Gly Trp Ile Lys Ile Ser Asp Cys Lys His Gln Val Gln Thr Ile
1070 1075 1080
His Phe Ser Ser Cys Asp Glu Asn Glu Ile His His Ser Ile Tyr
1085 1090 1095
Gln Thr Lys Ser Ile Pro Phe Thr Pro Thr Thr Asp Gln Ile Arg
1100 1105 1110
Ile Asp Ile Gly Glu Thr Glu Gly Ser Phe Thr Ile Arg Ser Ile
1115 1120 1125
Glu Leu Ile Cys Leu
1130
<210> 141
<211> 626
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 141
Met Asn Pro Asn Glu Glu Tyr Lys Thr Thr Val Pro His Ser Asn Lys
1 5 10 15
Ile Gln Pro Arg Tyr Pro Leu Thr Asn Asn Pro Asp His Pro Leu Gln
20 25 30
His Thr Asn Tyr Lys Asp Trp Leu Gln Met Cys Gln Asn Thr Ser Phe
35 40 45
Asp Gly Glu Asn Pro Val Pro Ser Val Thr Glu Val Gly Ala Ile Gly
50 55 60
Ile Ala Ile Ser Ile Val Ser Thr Leu Leu Ser Thr Phe Gly Ala Ala
65 70 75 80
Val Pro Gly Ala Ala Leu Ala Val Leu Gly Ala Thr Leu Pro Phe Phe
85 90 95
Asp Ser Gly Ser Gly Ser Val Trp Glu Asp Phe Met Ile His Asn Ser
100 105 110
Ser Val Leu Gln Lys Thr Leu Pro Glu Ser Ile Lys Asn Gln Ala Asn
115 120 125
Thr Ile Leu Thr Gly Leu Gln Glu Ser Leu Ile Glu Tyr Lys Arg Val
130 135 140
Phe Asn Ile Trp Lys Lys Asp Lys Thr Pro Ala His Ala Glu Gln Val
145 150 155 160
Arg Ser Thr Tyr Arg Asn Thr His Asn Leu Phe Ile Asn Arg Met Ser
165 170 175
Glu Leu Arg Pro Ser Gln Tyr Lys Gln Ile Leu Leu Ser Val Tyr Cys
180 185 190
His Ala Ala Asn Leu His Leu Gly Leu Leu Val Gln Gly Val Ala Tyr
195 200 205
Tyr Asn Glu Trp Tyr Asn Val Asn Pro Ser Leu Leu Thr Pro Asp Arg
210 215 220
Thr Val Asp Asp Glu Tyr Leu Leu Leu Gln Asp Lys Ile Lys Glu Tyr
225 230 235 240
Ile Asp Tyr Cys Ser Lys Thr Tyr Arg Glu Gly Leu Asn Ile Leu Ala
245 250 255
Asn Ser Gln Ala Asn Leu Lys Ile Tyr Thr Thr Tyr Gln Arg Glu Met
260 265 270
Thr Phe Thr Val Leu Asp Ile Ile Thr Asn Phe Pro Tyr Tyr Asp Phe
275 280 285
Lys Lys Tyr Pro Ile Gly Val Gln Ser Glu Leu Thr Arg Glu Ile Tyr
290 295 300
Thr Ile Glu Gln Gln Ile Lys Gln Ile Tyr Leu Asn His Leu Thr Arg
305 310 315 320
Gln Pro Asn Leu Val Thr His Pro Phe Asn Ser Ser Ile Tyr His Tyr
325 330 335
Arg Asn Leu Pro Phe Ile Thr Ser Gly Phe Thr Ala Val Arg Asn Arg
340 345 350
Phe Trp Phe Thr Asn Ala Arg Asn Leu Ser Val Ser Gly Leu Tyr Gly
355 360 365
Ser Thr Ser Phe Asp Gly Gln Phe Asp Val Ser Thr Gln Asn Val Trp
370 375 380
Lys Val Glu Ala Thr Tyr Ala Asp Val Ala Arg Arg Leu Lys Gln Leu
385 390 395 400
Lys Phe Tyr Ala Gln Leu Pro Asn Pro Ile Thr Ala Thr Thr Gly Thr
405 410 415
Pro Asn Asn Ser Asp Tyr Arg Glu Leu Phe Thr His Glu Ile Pro Gly
420 425 430
Arg Asp Gly Lys Leu Pro Thr Thr Phe Gln Thr Ala Ser His Tyr Leu
435 440 445
Ser Tyr Met Met Met Thr Ala Ser Glu Ser Pro Gly Leu Ser Cys Ala
450 455 460
Trp Arg His Ala Ser Val Asn Asn Asp Asn Leu Ile Ser Pro Asn Lys
465 470 475 480
Ile Thr Leu Ile Pro Ala Val Lys Ala Arg Glu Leu Asp Thr Ser Ser
485 490 495
Lys Val Val Ser Gly Pro Gly His Thr Gly Gly Asp Leu Val Glu Phe
500 505 510
Asn Met Ala His Gly Arg Leu Glu Leu Gln Gly Ser Ile Gly Asn Val
515 520 525
Ser Gln Glu Arg Phe Thr Ile Arg Leu Arg Tyr Ala Ala Ala Ala Asn
530 535 540
Glu Ser Pro Arg His Leu Leu Val Thr Ile Pro Asn Ile Gly Val Phe
545 550 555 560
Pro Val Met Leu Leu Pro Thr Thr Tyr Lys Leu Ser Leu Asn Glu Phe
565 570 575
Ser Tyr Asn Glu Phe Gly Phe Val Asp Ile Ile Pro Ser Asn Glu Ser
580 585 590
Ile Ser Phe Phe Ala Ser Ile Pro Trp Ile Val Ser Ile Thr Ser Thr
595 600 605
Arg Gly Ser Ile Asp Asp Lys Val Tyr Ile Asp Arg Leu Glu Phe Ile
610 615 620
Pro Ile
625
<210> 142
<211> 303
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 142
Met Asp Ser Lys Asn Ser Val Ser Lys Asn Gln Gln Tyr Met Ala Ala
1 5 10 15
Glu Glu Arg Pro Thr Val Pro Val Ser Thr Asp Glu Asp Tyr Gly Ile
20 25 30
Arg Ser Leu Ala Ile Asp Asn Lys Asp Thr Phe Tyr Asp Val Val Arg
35 40 45
Lys Ile Val Gly Trp Asn Gly Ile Gln Asp Leu Ser Ala Asn Phe Leu
50 55 60
His Tyr Ala Lys Pro Thr Tyr Ser Val Asp Leu Val Ala Glu Pro Lys
65 70 75 80
Asp Glu Pro Leu Ser Pro Val Leu Ile Gly Lys Thr Asn Ile Gln Asn
85 90 95
Lys Ser Gln Ser Thr Gln Lys Leu Gln Ser Ala Ser Met Ser Lys Thr
100 105 110
Val Thr Ser Thr Val Thr Ser Thr Val Thr His Gly Ala Lys Ala Gly
115 120 125
Ala Lys Ala Ser Ala Lys Leu Lys Val Pro Leu Val Ala Glu Ala Gly
130 135 140
Val Glu Leu Asn Thr Glu Tyr Asn Phe Ala Ser Ala Gly Ser Asn Thr
145 150 155 160
Ser Thr Glu Thr Leu Thr Tyr Thr Leu Pro Ser Gln Ser Ile Glu Leu
165 170 175
Glu Pro Gly Gln Arg Ala Thr Val Thr Ser Asp Leu Ser Met Met Lys
180 185 190
Thr Ser Gly Glu Val Gln Leu Arg Thr Lys Tyr Glu Gly Tyr Val His
195 200 205
Leu Lys Gly Thr Leu Ile Asn Thr Pro Asn Asn Ile Asp Asn Asn Ile
210 215 220
Lys Met Gly Asp Phe Val Asp Arg Phe Ile Lys Ala Asp Ser Ser Leu
225 230 235 240
Arg Glu Tyr Trp His Val Asn Ser Pro Asn Asp Phe Ile Ala Tyr Gln
245 250 255
Val Gly Lys Gly Asn Phe Thr Ala Glu Tyr Gly Thr Val Ala Asn Ile
260 265 270
Thr Val Glu Ile Phe Asn Gln Asp Gly Thr Asp Thr Thr Gln Pro Ile
275 280 285
Lys Ser Tyr Thr Tyr Thr Val Ile Pro Glu Val Ser Lys Asn Ile
290 295 300
<210> 143
<211> 194
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 143
Met Ala Ala Ser Phe Lys Thr Val Ile Glu Val Gly Pro Ala His Leu
1 5 10 15
Asp Gln Ala Gln Ala Leu Gly Arg Ala Phe Gln Asp Ala Ile Ala Pro
20 25 30
Ala Thr Val Asn Tyr Asp Phe Asp His Ile Arg Asp Ala Ala Ala Ala
35 40 45
Ile Pro Asp Ser Ser Ile Val Lys Met Val Arg Gly Trp Gly Leu Gln
50 55 60
Glu His Ala Pro Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val
65 70 75 80
Arg Gln Ala Leu Pro Pro Glu Ser Ala Glu Ala Ser Phe Trp Asp Thr
85 90 95
Val Glu Arg Glu Leu Val Asp Ala Phe Thr Gly Leu Ala Ala Gln Glu
100 105 110
Gly Leu Pro Gly Leu Ser Tyr Tyr Glu Glu Gly Ala Asp Arg Thr Arg
115 120 125
Tyr Tyr Arg Asp Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Gly
130 135 140
His Met Tyr Ala Ile Ala Phe Cys Ile Asp Val Thr Ile Gly Leu Asp
145 150 155 160
Lys Ala Arg Val Gly Ala Leu Glu Met Thr Asp Ala Val Pro Phe Ala
165 170 175
Leu Arg Leu Asn Ala Ile Val Leu Arg Gln Glu Leu Thr Arg Ala Leu
180 185 190
Thr Ala
<210> 144
<211> 364
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 144
Met Ala Thr Pro Glu Gln Asn Glu Tyr Tyr Asn Ile Lys Leu Lys Ala
1 5 10 15
Asn Thr Tyr Lys Ile Trp Asp Val Asp Asn Ala Ser Ser Asp Asn Asp
20 25 30
Arg Val Ile Arg Leu Trp Gly Gly Ser Thr Thr Glu Ala Asn Arg Gln
35 40 45
Phe Val Phe Phe Pro Leu Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys
50 55 60
Asn Ser Gly Lys Pro Val Thr Phe Gly Asn Gly Ala Trp Phe Ser Ile
65 70 75 80
Asn Gly Gly Ser Ser Ile Leu Ala Gly Ala Asp Ile Leu Gln Gln Gln
85 90 95
Ser Trp Thr Gly Ser Ala Asn Gln Gln Trp Tyr Leu Arg Asp Gln Gly
100 105 110
Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly Asn Gly Lys Val Ala Ser
115 120 125
Tyr Ala Trp Gly Gly Thr Leu Ala Gln Asn Trp Glu Tyr Val Asp Leu
130 135 140
Asp Glu Ser Lys Pro Tyr Asp Ser Asn Asn Val Phe Asn Ile Ala Asn
145 150 155 160
Ser Gly Ser Ser Ile Ser Leu Pro Asn Leu Pro Ala Lys Gly Asp Arg
165 170 175
Pro Asn Ala Pro Asp Tyr Asn Gln Thr Gly Arg Ile Asp Gln Gln Leu
180 185 190
Pro Gln Thr Ser Glu Ser Val Val Ile Gly Ala Ser Leu Ile Pro Ser
195 200 205
Ile Met Val Lys Asp Asn Ser Ala Ser Asp Tyr Thr Lys Ile His Asn
210 215 220
Ser Pro Tyr Tyr Val Leu Glu Lys Glu Glu Tyr Trp Glu Lys Val Arg
225 230 235 240
Ser Glu Ile Val Pro Ala Gly Gly Ser Ser Lys Tyr Thr Val Lys Arg
245 250 255
Gly Val Ser Thr Val Asp Gln Gln Lys Met Thr Asp Thr Leu Asn Met
260 265 270
Lys Phe Gly Thr Asp Leu Gly Phe Lys Phe Gly Asp Thr Thr Ala Ser
275 280 285
Ile Lys Ser Glu Ile Ser Lys Thr Leu Gln Thr Glu Val Ser Lys Thr
290 295 300
Ser Thr Asp Ala Thr Glu Glu Thr Lys Glu Lys Gly Phe Ser Ser Thr
305 310 315 320
Ser Gly Lys Asp Thr Gly Leu Thr Val Tyr Gln Leu Val Thr Lys Tyr
325 330 335
Thr Leu Lys Arg Thr Asp Gly Ser Thr Val Ser Ser Ser Tyr Val Arg
340 345 350
Asp Gly Asp Gln Thr Leu Glu Arg Thr Ile Ala Lys
355 360
<210> 145
<211> 297
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 145
Met Leu Asn Gln Gly Gly Ala Gly Asn Ser Gly Ser Gly Tyr Leu Val
1 5 10 15
Asn Asn Met Ser Glu Lys His Glu Asp Ile Ile Asp Pro Glu Asp Ile
20 25 30
Phe Ser Asn Met Leu Asn Ala Val Gly Lys Tyr Lys Glu His Lys Asp
35 40 45
Pro Phe Leu Phe Val Thr Arg Asn Ser Ile Gly Ser Leu Ser Ser Tyr
50 55 60
Ser Asn Phe Asn Ala Glu Gln Asp Gly Asp Pro Ile Ile Glu Asn Glu
65 70 75 80
Ser Leu Leu Phe Val Gly Glu Ser Ile Leu Thr Asn Asn Met Asn Thr
85 90 95
Glu Gln Thr Leu Lys Thr Asn Ser Phe Ser Lys Thr Met Ser Asn Thr
100 105 110
Val Ser Ser Gln Val Thr His Gly Phe Met Leu Gly Thr Glu Ala Arg
115 120 125
Thr Ser Phe Gly Ile Pro Leu Ile Gly Glu Thr Glu Leu Ser Leu Lys
130 135 140
Ala Glu Tyr Asn Phe Ser Ser Ser Glu Thr Asn Glu Ile Ser Glu Ser
145 150 155 160
Tyr Thr Tyr Gly Val Glu Pro Gln Glu Ile Leu Val Pro Pro His Ser
165 170 175
Ser Val Lys Val Arg Val Asp Leu Asn Thr Ala Asp Ile Ser Gly Lys
180 185 190
Val Lys Leu Met Ala Thr Pro Lys Thr Asp Phe Gly Asp Ile Asp Gly
195 200 205
Tyr Phe His Tyr Gly Pro Asp Glu Gln Asn Ile Ala Glu Ser Ile Pro
210 215 220
Phe Thr Tyr Asn Trp Asn Thr Leu Thr Lys Glu Val Arg Gln Leu Asn
225 230 235 240
Leu Pro Val Asp Asp Ile Asp Tyr Asp Phe Asn Asn Gln Thr Pro Lys
245 250 255
Leu Val Gly Ser Gly Lys Tyr Lys Ala Lys Tyr Gly Thr Thr Phe Lys
260 265 270
Val Glu Val Ile Pro Ile Asn Asn Pro Lys Ala Glu Gly Tyr Ser Tyr
275 280 285
Ser Ile Gln Pro Thr His Thr Ala Asn
290 295
<210> 146
<211> 494
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 146
Met Ala Glu Lys Pro Pro Ala Gln Gln Gln Pro Gln Ser Ser Val Lys
1 5 10 15
Ser Val Gly Gly Ala Asp Asp Val Tyr Ser Asn Glu Ile Asn Asn Pro
20 25 30
Pro Phe Lys Ile Glu Gly Asp Ala Leu Thr Leu Thr Lys Asn Phe Leu
35 40 45
Ser Gly Asp Leu Ala Ala Thr Ala Lys Lys Met Trp Gly Ser Ser Leu
50 55 60
Asn Leu Ala Val Gln Ser Ala Gln Gly Lys Asn Val Leu Asn Val Asn
65 70 75 80
Asn Leu Ala Arg Asp Met Val Met Val Gly Thr Ser Ala Ile Pro Tyr
85 90 95
Gly Gly Val Ile Leu Ser Ser Ile Ile Gly Val Leu Trp Pro Met Ser
100 105 110
Gly Thr Asn Gln Met Gln Ala Leu Met Asp Lys Met Gly Gln Ile Met
115 120 125
Asp Gln Lys Ile Glu Lys Tyr Asp Asn Thr Thr Leu Thr Ser Lys Ile
130 135 140
Asn Ala Val Glu Val Ala Leu Lys Arg Phe Glu Thr Ile Leu Tyr Gly
145 150 155 160
Thr Asp Asp Val Tyr Ser Asp Thr Asn Lys Ala Gly Glu Ala Leu Arg
165 170 175
Gln Ala Thr Val Ile Gln Asn Ala Phe Thr Glu Leu Ile Asn Glu Ser
180 185 190
Lys Lys Glu Glu Tyr Glu Thr Ser Glu Leu Pro Ala Tyr Thr Met Ala
195 200 205
Ala Thr Ala His Leu Leu Phe Leu Ser Phe Met Val Thr Asn Gly Thr
210 215 220
Ser Ile Leu Gly Leu Glu Pro Asp Thr Met Lys Asn Ser Tyr Asn Asn
225 230 235 240
Asp Leu Lys Asn Ala Leu Ser Glu Tyr Asn Ser His Ile Gln Arg Ile
245 250 255
Gly Ser Asp Ser Asp Tyr Lys Leu Ser Gln Leu Pro Gln Lys Ile Asp
260 265 270
Ala Ala Arg Val Glu Ile Glu Lys Ala Lys Ala Gly Met Asn Pro Pro
275 280 285
Ile Gly Ala Ala Ala Gly Tyr Trp Ala Glu Ile Tyr Gln Gln Lys Ser
290 295 300
Gln Glu Leu His Asn Leu Leu Glu Gln Gln Lys Gln Leu Thr Asp Leu
305 310 315 320
Val Asn Ser Thr Thr Lys Asn Glu Ala Phe Ala Ala Ala Leu Lys Ser
325 330 335
Ala Lys Thr Thr Ala Asp Asn Lys Gly Ile Lys Tyr Glu Glu Lys Asp
340 345 350
Ser Ser Lys Ile Val Asp Gly Gly Thr Tyr Lys Ile Ile Ser Ala Leu
355 360 365
Asn Asn Gly Gln Tyr Val Asp Asn Gln Arg Glu Thr Lys Asn Val Ser
370 375 380
Ile Tyr His Asn Thr Arg Ser Asp Asn Gln Asn Trp Lys Phe Ile Phe
385 390 395 400
Asp Ser Ser Lys Gln Ala Tyr Lys Ile Gln Ser Val Tyr Asp Ser Arg
405 410 415
Val Leu Ala Thr Ser Ser Ser Ala Asn Tyr Asn Val Phe Thr Tyr Asn
420 425 430
Phe Asp Gly Ser Glu Asp Gln Phe Trp Ile Pro Glu Asp Ile Gly Asn
435 440 445
Gly Thr Tyr Ile Phe Lys Asn Lys Lys Tyr Pro Asp Arg Val Leu Asp
450 455 460
Ile Phe Glu Gly Lys Ala Glu Asp Ser Thr Asn Ile Thr Ala Tyr Pro
465 470 475 480
Arg Asn Asn Gly Ala Gly Gln Lys Phe Lys Val Ile Gln Gln
485 490
<210> 147
<211> 390
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 147
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Gly Val Gly Ala
1 5 10 15
Met Ser Leu Ser Ile Trp Ala Pro Ser Ser Gln Ala Ala Ala Pro Ala
20 25 30
Asn Asn Glu Tyr His Thr Ile His Leu Ala Ala Asn Thr Asn Ile Ala
35 40 45
Trp Asp Val Tyr Lys Gly Trp Gln Thr Asp Asp Ala Gly Ile Val Leu
50 55 60
Trp Asp Gly Val Arg Gly Asp Asn Glu Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Ile Gly Ser Ala Ile Ile Gly Ser Asp Gly Val Arg Asn Phe Pro
100 105 110
Ser Tyr Asn Asn Gln Gly Leu Arg Gln Thr Ser Trp Thr Gly Ala Pro
115 120 125
Asn Glu Gln Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Phe Glu Ile
130 135 140
Val Asn Gln Gly Ser Gly Lys Val Ala Ser Trp Ala Gly Val Glu Met
145 150 155 160
Tyr Gly Phe Lys Asp Tyr Val Asp Leu Asp Glu Ser Asn Pro Ser Asp
165 170 175
Lys Asp Arg Leu Phe Asn Ile Pro Ala Ala Arg Ser Thr Phe Ser Leu
180 185 190
Pro Thr Leu Pro Thr Val Gly Thr Arg Pro Asn Ala Pro Asp Tyr Asn
195 200 205
Thr Ser Gly Ala Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val
210 215 220
Val Val Gly Ala Ser Leu Ile Pro Ser Ile Met Val Lys Asp Asn Gly
225 230 235 240
Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Val Leu Val
245 250 255
Lys Glu Glu Tyr Trp Glu Lys Val Arg Ser Glu Ile Ile Pro Ala Gly
260 265 270
Ala Thr Ser Lys Tyr Thr Val Lys Ser Gly Val Ser Glu Ile Asp Gln
275 280 285
Lys Lys Met Ser Asp Thr Leu Ala Met Ser Phe Gly Ser Asp Leu Gly
290 295 300
Leu Lys Phe Gly Glu Ser Ser Leu Ser Ile Lys Ser Ser Val Ser Arg
305 310 315 320
Thr Ile Gln Thr Glu Val Thr Lys Thr Ser Thr Asn Ala Thr Glu Glu
325 330 335
Thr Glu Glu Lys Ser Ile Pro Ser Ile Pro Gly Lys Asn Thr Gly Phe
340 345 350
Thr Ala Tyr Gln Leu Val Thr Lys Tyr Thr Leu Lys Arg Thr Asp Gly
355 360 365
Ser Val Val Thr Asp Pro Ser Ile Val Arg Asp Ser Lys Gln Thr Ile
370 375 380
Ala Arg Thr Ile Ala Lys
385 390
<210> 148
<211> 387
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 148
Met Lys Ser Ile Ser Lys Lys Ile Met Ala Gly Leu Gly Val Gly Ala
1 5 10 15
Met Ser Leu Ser Ile Trp Ala Pro Ser Ser Glu Ala Ala Ala Pro Ala
20 25 30
Lys Asn Glu Tyr His Thr Ile His Leu Ala Ala Asn Thr Asn Leu Ala
35 40 45
Trp Asp Val Asp Asn Gly Ser Val Asp Asn Asp Arg Ala Ile Leu Leu
50 55 60
Trp Asn Gly Asp Arg Asn Asp Asn Glu Gln Phe Leu Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Ser Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Thr Phe Gly Gly Pro Thr Trp Ser Gly Ser Gly Gly Ile Leu Gly Asn
100 105 110
Gln Asn Leu Asn Gln Arg Ser Trp Thr Gly Ser Ser Asn Glu Gln Trp
115 120 125
Tyr Leu Arg Asp Arg Gly Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly
130 135 140
Asn Gly Lys Val Ala Ser Tyr Ala Tyr Gln Trp Ala Ser Arg His Ile
145 150 155 160
Glu His Val Asp Leu Asp Glu Ser Asn Pro Ser Asp Asn Asp Arg Leu
165 170 175
Phe His Ile Pro Ala Ala Ala Arg Ser Thr Phe Ser Leu Pro Thr Leu
180 185 190
Pro Ala Val Gly Thr Arg Pro Val Ala Pro Glu Tyr Thr Gln Gly Gly
195 200 205
Ser Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly
210 215 220
Ala Ser Leu Val Pro Cys Ile Met Val Lys Asp Ser Gln Ala Ser Asp
225 230 235 240
Tyr Lys Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu
245 250 255
Tyr Trp Asp Lys Thr Phe Ser Ala Val Ile Pro Ala Gly Leu Thr Arg
260 265 270
Asn Tyr Thr Phe Lys Thr Gly Met Thr Ser Val Asp Gln Gln Lys Met
275 280 285
Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Phe Gly Leu Lys Phe
290 295 300
Gly Asp Ala Ser Ala Ser Ile Lys Ser Glu Ile Ser Lys Thr Ile Gln
305 310 315 320
Thr Glu Ile Ser Thr Thr Asn Thr Glu Ala Thr Glu Glu Thr Ile Thr
325 330 335
Ser Thr Val Thr Ser Glu Leu Gly Lys Thr Thr Gly Phe Thr Glu Tyr
340 345 350
Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Val Val
355 360 365
Ser Asp Pro Trp Val Val Lys Asn Asn Lys Ile Thr Val Ala Arg Lys
370 375 380
Asn Ser Ile
385
<210> 149
<211> 271
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 149
Met Asp Val Ser Asn Lys Asn Ser Ala Tyr Gln Asp Ile Gly Glu Arg
1 5 10 15
Val Lys Lys Met Ala Gln Ser Ala Ala Trp Gly Gly Gln Glu Tyr Arg
20 25 30
Ser His Asn Ile Lys Asp Ile Glu Leu Lys Gly Asn Leu Ile Asp Gly
35 40 45
Ser Met Val Glu Asn Ser Gln Val Leu Thr Val Ser Ser Asp Val Leu
50 55 60
Glu Asn Asn Leu Gly His Thr Val Asn Met Pro Ser Thr Gly Tyr Glu
65 70 75 80
His Glu Phe Glu Glu Thr Thr Ser Thr Thr Asn Thr Ser Gly Trp Lys
85 90 95
Phe Gly Tyr Asn Tyr Asn Ala Ser Phe Ser Val Leu Met Val Ser Ala
100 105 110
Ser Gln Ser Phe Ser Val Glu Tyr Asp Met Ser Thr Ser Asp Thr His
115 120 125
Glu Thr Lys Glu Lys Arg Lys Phe Thr Val Pro Ser Ile Glu Val Pro
130 135 140
Val Pro Ala Gly Lys Lys Tyr Lys Val Glu Tyr Val Phe Glu Lys Val
145 150 155 160
Lys Val Ser Gly Lys Asn Lys Ile Asp Ala Ser Leu Phe Gly Asp Val
165 170 175
Thr Tyr Tyr Tyr Asn Asn Gln Pro Met Ser Pro Gln Leu Leu Tyr Ser
180 185 190
Val Gln Gly Leu Ala Thr Asp Lys Gln Gly Phe Glu Gln Val Ile Arg
195 200 205
Asp Ser Ala Ile Gly Asn Asp Arg Phe Gly Ile Lys Thr Thr Gly Ile
210 215 220
Gly Gln Phe Ser Thr Glu Phe Gly Thr Arg Leu Thr Arg Thr Leu Thr
225 230 235 240
Asp Ile Thr Asp Ala Arg Asn Pro Val Lys Leu Glu Thr Lys Asn Ile
245 250 255
Pro Val Glu Phe Lys Thr Leu Ser Ile Asp Thr Arg Val Ile Lys
260 265 270
<210> 150
<211> 314
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 150
Met Asp Val Ile Asn Leu Lys Lys Gly Phe Glu Gln Gln Lys Gln Ile
1 5 10 15
Ala Ala Asp Val Asp Pro Ile Pro Val Pro Ile Glu Asn Val Lys Gly
20 25 30
Leu Gln Asp Leu Trp Leu Asp Ser Lys Glu Met Lys Cys Pro Leu Ala
35 40 45
Asp Leu Ile Gly Thr Ala Pro His Asn Leu Ser Asn Leu His Phe Asn
50 55 60
Phe Leu Tyr Tyr Lys Glu Pro Ser Tyr Ser Val Asp Val Ile Lys Lys
65 70 75 80
Asn Glu Glu Lys Pro Ser Ser Pro Val Tyr Met Gly Thr Thr Asn Leu
85 90 95
Ser His Ser Gly Gly Thr Ser Glu Gln Thr Leu Tyr Ser Gln Ala Phe
100 105 110
Ser Lys Thr Val Thr Ser Ser Val Thr Thr Thr Ile Thr His Gly Ala
115 120 125
Lys Val Gly Ala Lys Ala Gly Ala Lys Leu Gln Val Pro Leu Val Gly
130 135 140
Glu Thr Ser Val Glu Leu Asn Ala Glu Tyr Asn Phe Ala Lys Ala Gly
145 150 155 160
Ser Glu Thr Gly Thr Glu Asn Val Thr Tyr Thr Val Pro Ser Gln Pro
165 170 175
Val Lys Leu Gln Pro Gly Glu Ser Ala Lys Ile Thr Ala Asn Leu Arg
180 185 190
Met Ala Lys Ala Ser Gly Glu Val Arg Leu Arg Thr Thr Tyr Thr Gly
195 200 205
Glu Ile Gln Ser Asn Phe Phe Val Asn Thr Pro Tyr Gly Pro Met Glu
210 215 220
Asp Ser Asp Thr Thr Gly Leu Gly Glu Trp Met Lys Thr Leu Val Asp
225 230 235 240
Phe Asn Glu Asp Leu Lys Asn Gln Trp Thr Phe Ser Pro Thr Tyr Ala
245 250 255
Ser Val Ala Tyr Gln Asp Gly Lys Gly Thr Tyr Asn Ala Thr Tyr Gly
260 265 270
Ala Val Leu Asp Ile His Val Asp Ile Phe Gly Gly Ser Asn Lys Gly
275 280 285
Arg Lys Lys Arg Asp Ala Asp Ser Thr Pro Ile Arg Ser Tyr Ser Tyr
290 295 300
Glu Val Thr Pro Glu Ile Val Glu Asn Asn
305 310
<210> 151
<211> 300
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 151
Met Glu Thr Pro Ala Ile Asn Ser Lys Ala Lys Glu Ser Tyr Tyr Ser
1 5 10 15
Gly Thr Ile Asp Leu Met Phe Ile Asn Glu Phe Asn Lys Asn Cys Leu
20 25 30
Ser Ile Asp Asn Asn Asn Ser Arg Thr Ile Ile Lys Asp Leu Lys Pro
35 40 45
Ala Arg Glu Gln Thr Glu Ser Ile Asp Ser Phe Glu Val Asp Asn Ser
50 55 60
Asn Ser Asn Ser Thr Arg Ile Phe Lys Ser Gln Gln Lys Thr Tyr Lys
65 70 75 80
Asn Thr Asp Thr Val Thr Val Thr His Ala Lys Glu Phe Ser Phe Gly
85 90 95
Gln Glu Ile Ser Ala Glu Leu Gly Ala Pro Lys Leu Leu Glu Asn Leu
100 105 110
Leu Asn Met Ala Lys Ile Asn Leu Lys Ala Thr Phe Gly Gln Lys Ile
115 120 125
Thr Asn Ser Lys Ala Glu Ala Lys Ser Gln Glu Gln Ser Thr Thr Tyr
130 135 140
Gly Gly Asp Asn Ile Glu Val Lys Pro Gly Gln Lys Val Lys Val Glu
145 150 155 160
Tyr Glu Tyr Ile Thr Asn Ile Tyr Ser Gly Thr Leu Glu Thr Leu Ala
165 170 175
Pro Leu Lys Tyr Asn Val Lys Asp Pro Ser Met Ser Tyr Val Ser Pro
180 185 190
Lys Asp Ile Pro Tyr Tyr Lys Lys Thr Leu Pro Tyr Val Ala Lys Leu
195 200 205
Ser Gly Gly Lys Lys Tyr Ser Thr Asp Tyr Glu Phe Phe Lys Asp Val
210 215 220
His Asn Thr Pro Asp Asp Trp Ala Val Leu Ile Ala Tyr Asp Lys Gly
225 230 235 240
Asp Ala Tyr Phe Phe Tyr Ala Trp Glu Leu Lys Asp Thr Thr Thr Leu
245 250 255
Asn Asp Asn Lys Lys Gln Val Tyr Ser Lys Gly Ser Ala Lys Phe Glu
260 265 270
Ala Val Arg Gly Thr Gly Val Lys Val Lys Val Thr Asp Leu Asp Thr
275 280 285
Gly Lys Ile Val Lys Asp Lys Val Val Ser Tyr Phe
290 295 300
<210> 152
<211> 557
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 152
Met Glu Glu Lys Asp Lys Asn Ile Ala Gln Gln Gly Ala Glu Gln Gly
1 5 10 15
Glu Pro Ala Glu Lys Asp Thr Thr Ala Thr Gln Gln Gln Asn Thr Val
20 25 30
Gln Gln Lys Asn Pro Gly Thr Tyr Tyr Glu Asp Glu Lys Leu Asn Pro
35 40 45
Ile Ala Gln Leu Asp Lys Trp Thr Gln Asp Leu Gly Lys Asn Thr Gly
50 55 60
Ala Ala Asn Tyr Lys Thr Ser Leu Ser Ala Val Glu Lys Ile Met Pro
65 70 75 80
Leu Leu Ile Lys Asp Ile Thr Lys Asp Asn Tyr Asn Asn Ser Leu Arg
85 90 95
Ala Val Thr Met Phe Gly Ala Ala Leu Ile Pro Tyr Gly Gly Ser Phe
100 105 110
Ile Ser Pro Leu Ile Gly Leu Ile Trp Pro Glu Ser Lys Asp Asp Ala
115 120 125
Ala Leu Lys Leu Lys Lys Gln Leu Asp Asp Leu Arg Lys Glu Leu Ser
130 135 140
Asp Leu Met Asp Glu Lys Ile Asp Arg Phe Asp Gln Glu Gly Ile Gln
145 150 155 160
Ala Ala Ala Asn Val Ile Asn Glu Asn Leu Lys Lys Leu Glu Asp Gly
165 170 175
Ile Ala Gly Lys Thr Pro Gly Ile Phe Tyr Asp Asn Gln Lys Lys Leu
180 185 190
Glu Asp Gly Ile Ala Gly Lys Ser Glu Gly Ser Phe Tyr Asp Ser Gln
195 200 205
Ser Gly Phe Thr Val Val Tyr Ser Ile Asn Ser Ala Phe Glu Leu Leu
210 215 220
Leu Ala Asn Ala Ser Lys Pro Ser Phe Lys Met Gln Glu Leu Pro Leu
225 230 235 240
Tyr Thr Gly Leu Ala Ala Ala His Leu Gln Phe Leu Glu Phe Val Lys
245 250 255
Gln Asn Gly Lys Asn Pro Lys Ile Ala Leu Thr Asn Glu Asn Tyr Asn
260 265 270
Thr Phe Val Asn Lys Ile Ala Glu Lys Thr Pro Val Tyr Thr Gln His
275 280 285
Ile Glu Asp Thr Tyr Asn Gln Phe Arg Ser Lys Ile Glu Gln Lys Met
290 295 300
Thr Pro Leu Leu Gln Gly Val Ile Gly Val Thr Ser Asp Glu Gln Gln
305 310 315 320
Asn Leu Gly Ala Ala Phe Asp Lys Thr Ile Glu Leu Gln Arg Lys Val
325 330 335
Val Gln Ile Thr Gly Gly Asp Arg Leu Gly Ile Asn Ser Lys Asp Lys
340 345 350
Gln Lys Leu Gln Thr Phe Ile Asp Asn Lys Glu Asn Tyr Lys Lys Leu
355 360 365
Met Glu Thr Lys Ser Asp Tyr Tyr Asn Ser Thr Val Gly Leu Phe Ala
370 375 380
Val Thr Gln Gly Ile Pro Ser Lys Ile Gln Lys Pro Glu Thr Thr Pro
385 390 395 400
Thr Trp Gly Pro Leu Lys Thr Leu Gln Gly Pro Val Ala Asn Gly Thr
405 410 415
Tyr Lys Ile Ala Ser Lys Leu Asp Ser Asn Met Leu Leu Asp Asn Asp
420 425 430
His Asn Arg Asn Arg Leu Ala Thr Leu Asn Lys Asp Ile Thr Thr Lys
435 440 445
Asp Asn Leu Trp Gly Leu Gly Trp Glu Leu Arg Tyr Asn Pro Asp Lys
450 455 460
Gln Ala Tyr Lys Phe Ile Ser Lys Leu Gly Ser Asp Glu Leu Ala Thr
465 470 475 480
Asn Gly Gln Asp Asp Thr Val Phe Ala Thr Thr Asp Arg Asn Asp Asn
485 490 495
Asp Leu Arg Tyr Trp Thr Leu Glu Pro Ala Ala Glu Asp Gly Tyr Phe
500 505 510
Phe Ile Lys Asn Lys Ala Ser Gly Arg Val Leu Asp Val Thr Gly Gly
515 520 525
Asn Thr Ala Ala Gly Thr Asp Ile Lys Thr Asn Val Gln Asn Thr Pro
530 535 540
Ala Val Ala Ala Gln Leu Phe Lys Leu Glu Thr Val Gln
545 550 555
<210> 153
<211> 271
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 153
Met Ser Glu His Gly Lys Asn Asn Val Tyr Gln Asp Leu Asp Glu Arg
1 5 10 15
Val Lys Arg Met Leu Ile Ser Ala Ala Ala Pro Gly Thr Glu Tyr Arg
20 25 30
Tyr His Asn Ile Ile His Ala Glu Leu Asn Gly Gly Asp Val Glu Gly
35 40 45
Thr Leu Ile Glu Asn Ser Glu Thr Leu Thr Val Ser Arg Asn Thr Leu
50 55 60
Glu Asn Asn Phe Asp His Glu Ile Glu Leu Pro Thr Ser Gly Tyr Glu
65 70 75 80
His Glu Phe Thr Glu Thr Thr Ser Thr Thr Asn Ser Thr Gly Trp Thr
85 90 95
Phe Gly Tyr Asn Tyr Asn Val Glu Ile Ser Ile Pro Met Val Ser Gly
100 105 110
Gly His Ser Phe Ser Val Asp Tyr Asn Met Ser Thr Ala Asn Thr Val
115 120 125
Glu Lys Ser Glu Thr Arg Arg Phe Ser Val Pro Ser Val Leu Ile Pro
130 135 140
Val Pro Ala Gly Arg Lys Tyr Leu Val Glu Tyr Arg Phe Glu Lys Gln
145 150 155 160
Thr Val Ser Gly Arg Asn Arg Ile Asn Ala Asp Leu Phe Gly Asp Ala
165 170 175
Val Tyr Tyr Phe Asn Asn Gln Pro Met Ser Pro Gln Leu Leu Tyr Ser
180 185 190
Ala Leu Arg Phe Ala Thr Asp Thr Gln Gly Phe Glu Gln Val Ile Arg
195 200 205
Asp Ser Ala Val Gly Asn Asp Arg Phe Gly Ile Arg Gly Thr Gly Val
210 215 220
Gly Gln Phe Arg Thr Glu Phe Gly Thr Ser Leu Tyr Ile Arg Thr Thr
225 230 235 240
Asp Ile Thr Asn Ser Gln Glu Pro Val Ile Ile Glu Thr Ile Thr Ile
245 250 255
Pro Val Lys Ile Glu Thr Ile Ser Glu Lys Thr Lys Val Val Glu
260 265 270
<210> 154
<211> 292
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 154
Met Asp Gln Ile Ser Pro Ile Gln Lys Ile Thr Gln Glu Gln Gln Asn
1 5 10 15
Val Ala Ile Ser Asp Trp Arg Glu Pro Phe Gln Lys Thr Tyr Lys Ser
20 25 30
Ala Lys Leu Arg Asp Pro Asn Arg Tyr Phe Ser Asn Glu Ala Lys Ile
35 40 45
Asp Leu Trp Gly Val Tyr Gln Ser Ser Val Glu Ala Asp Gly Ser Pro
50 55 60
Thr Ile Thr Asn Ser Lys Ser Leu Phe Val Gly Arg Thr Ile Leu Thr
65 70 75 80
Asn His Thr Asn Gln Ala Gln Thr Leu Thr Thr Asn Glu Phe Ser Lys
85 90 95
Ser Phe Gly Asn Ser Val Thr Asn Ser Thr Thr Asn Gly Phe Asn Leu
100 105 110
Gly Val Ser Thr Ser Ala Ser Phe Gly Ile Pro Leu Ile Gly Glu Thr
115 120 125
Gly Ile Glu Ile Ser Thr Glu Tyr Asn Phe Ser Asn Thr Ala Glu Lys
130 135 140
Thr Lys Thr Glu Ser Tyr Met Tyr Thr Ala Ser Pro Gln Asn Ile Leu
145 150 155 160
Val Pro Ala Asn Ser Ser Val Glu Val Ile Val Asn Leu Asn Thr Ala
165 170 175
Lys Leu Asn Gly Asn Val Lys Leu Leu Ser His Val Asn Gly Gln Leu
180 185 190
Met Trp Asn Thr Ser Lys Thr Gly Gln Lys Ile Ser Thr Ser Phe Lys
195 200 205
Gly Phe Leu Glu Lys Ala Ala Arg Pro Asn Glu Ile Ala Ser Asn Val
210 215 220
Lys Ala His Pro Arg Asn Gly Ala Tyr Leu Ile Gly Lys Gly Lys Tyr
225 230 235 240
Ser Ala Glu Tyr Gly Thr Glu Phe Ser Val Thr Val Arg Pro Val Gln
245 250 255
Lys Pro Ser Thr Val Ala Ala Lys Ser Lys Ser Met Val Ser Glu His
260 265 270
Ser Lys Thr Thr Ser Glu Ser Tyr Thr Tyr Thr Val Lys Pro Glu Val
275 280 285
Lys Lys Glu Gln
290
<210> 155
<211> 349
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 155
Met Asn Thr Gln Glu Ser Thr Pro Ser Val Tyr Pro Ile Gln Gly Thr
1 5 10 15
Glu Phe Ser Asp Glu Asn Gly Gln Val Ile Ser Pro Gly Ala Phe Ala
20 25 30
Thr Leu Ser Pro Tyr Trp Leu Ile Asn Asp Ser Asn Asp Gln Leu Leu
35 40 45
Asn Phe Tyr Asn Gln Val Lys Gln Arg Leu Leu Asp Lys Gln Asn Thr
50 55 60
Glu Gln Asn Met Leu Lys Leu Asp Glu Tyr Leu Asn Asp Leu Trp Phe
65 70 75 80
Val Gly Ala Val Ser Glu Leu Tyr Ala Thr Pro Thr Asp Tyr Asn Lys
85 90 95
Arg Leu Glu Ala Ser Gln Leu Val Arg Trp Tyr Lys Asn Asn Asn Ala
100 105 110
Ala Arg Pro Thr Phe Glu Ile Leu Glu Ser Lys Leu Glu Asn Val Glu
115 120 125
Pro Ile Leu Asp Ile Thr Glu Pro Ile Gly Tyr Phe Ala Thr Glu Ser
130 135 140
Glu Tyr Thr Asn Asn Ser Pro His Gln Gly Glu Tyr Leu Ile Pro Gly
145 150 155 160
Trp Ser Asn Thr Thr Ser Thr Thr Tyr Asn Thr Thr Thr Thr Lys Gly
165 170 175
Phe Ser Ile Gly Ala Asn Thr Gly Ile Glu Ile Pro Leu Pro Phe Met
180 185 190
Asp Lys Leu Gly Leu Thr Gly Ser Tyr Asn Phe Ser Lys Ala Glu Asn
195 200 205
Val Ser Thr Thr Asp Ser Thr Thr Leu Thr Ile Pro Val Gln Thr Ile
210 215 220
Gln Val Ser Pro Phe Ser His Ser Lys Thr Ser Phe Tyr Phe Gln Thr
225 230 235 240
His Gln Tyr Arg Ala Lys Phe Asp Val Thr Ser Lys Phe Lys Ala Asp
245 250 255
Val Gly Arg Asp Ala Glu Tyr Thr Lys Lys Arg Ser Ala Phe Tyr Leu
260 265 270
Leu Ala Lys Tyr Asn Glu Tyr Leu Pro Lys Gly Ile Val Val Glu Asn
275 280 285
Gly Gly Lys Leu Thr Leu Glu Gly Lys Gly Glu Ile Ser Val Pro Asn
290 295 300
His Ile Asn Gly Phe Lys Leu Val Lys Glu Thr Lys Glu Thr Pro Leu
305 310 315 320
Leu Gly Gly Glu Gly Lys Thr Thr Lys Glu Ile Tyr Thr Lys Asp Pro
325 330 335
Asn Thr Asn Lys Leu Ile Pro Asp Ala Ser Ser Lys Ser
340 345
<210> 156
<211> 945
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 156
Met Gln Met Val Thr Lys Ala Met Asn Pro Lys Leu Gly Ala Leu Pro
1 5 10 15
Asp Phe Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Gly Asn Ile
20 25 30
Thr Asp Val Met Ser Thr Ile Phe Gly Val Asp Thr Gly Asp Ser Ser
35 40 45
Ile Asp Asp Val Leu Asn Asn Glu Glu Leu Leu Lys Gln Met Met Glu
50 55 60
Gln Met Asp Thr Met Gln Asp Ser Leu Glu Asp Ile Leu Lys Asn Gln
65 70 75 80
Glu Ile Ser Lys Asn Ile Gln Glu Gln Ile Leu Ala Leu Thr Thr Asp
85 90 95
Leu Ala Thr Ser Ile Asn Lys Glu Leu Val Lys Ile Glu Gly Ile Leu
100 105 110
Thr Thr Tyr Leu Asn Ala Ile Ser Gly Met Leu Lys Asp Ile Tyr Ser
115 120 125
Gln Thr Ser Val Ile Asp Gln Lys Val Asp Lys Leu Leu Ala Met Met
130 135 140
Thr Phe Ala Leu Lys Glu Leu Asp Tyr Ile Lys Asp Asn Val Val Leu
145 150 155 160
Asn Ser Ser Ile Val Glu Ile Thr Pro His Val Gln Lys Leu Val Tyr
165 170 175
Val Asn Lys Lys Phe Leu Ser Leu Thr Arg Gly Phe Leu Gln Gly Glu
180 185 190
Val Leu Ser Ile Asp Ser Met Gln Glu Ile Gln Glu Trp Ala Lys Ser
195 200 205
Ile Leu Ala Thr Glu Met Asn Ser Phe Glu Phe Ser Val Asp Thr Leu
210 215 220
His Asn Ile Ile Ile Gly Asp Asn Leu Tyr Lys Arg Ser Ala Leu Lys
225 230 235 240
Thr Phe Ser Asp Val Leu Leu Asp Asp Ala Asp Gln Tyr Gly Asp Phe
245 250 255
Gly Thr Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser Ser Leu Ala Thr
260 265 270
Leu Gln Ile Asn Ala Tyr Leu Cys Leu Thr Phe Ala Arg Lys Val Leu
275 280 285
Gly Leu Ser Glu Ile Asp Tyr Gln Val Thr Met Gln Asp Arg Ile Glu
290 295 300
Gln Gln Asn Gln Met Phe Val Asn Leu Ile Gln Asp Lys Asn Tyr Ser
305 310 315 320
Asn Ala Leu Glu Thr Lys Gly Ile Tyr Pro Met Pro His Asn Val Gly
325 330 335
Asp Cys Asn Ser Phe Asp Leu Gln Ala Lys Asn Gly Tyr Ala Leu Ile
340 345 350
Gly Leu Glu Phe Phe Met Asp Asn Asn Ile Tyr Lys Ile Lys Ala Tyr
355 360 365
Gln Gly Lys Ile Asp Lys Asn Phe Ser Leu Tyr Ala Asp Thr Val Glu
370 375 380
Glu Ile Ile Ser Asp Asp Leu Ser Ala Val Phe Pro Ala Ile Thr Glu
385 390 395 400
Asn Ala Pro Arg Leu Asp Ile Val Phe Pro Leu Ser Gly Glu Leu Ile
405 410 415
Gly Pro Pro Asn Thr Val Ile Thr Arg Ile Gly Leu Gly Thr Lys Tyr
420 425 430
Asp Lys Asn Arg Glu Ser Asp Val Arg Thr Phe Ala Tyr Ile Asp Ala
435 440 445
Asp Phe Ser Leu Tyr Asp Tyr Ile Ser Gly Thr Ile Ser Lys Glu Gly
450 455 460
Thr Gln Thr Val Ala Leu Glu Gly Asn Ala Gln Asn Gly Cys Arg Cys
465 470 475 480
Tyr Asp Met Trp Pro Ile Gly Leu Ile Gly Asp Leu Tyr Met Thr Pro
485 490 495
Leu Lys Gly Leu Ser Leu Thr Val Asp Glu Gly Thr Leu Asn Met Ser
500 505 510
Gly Glu Ser Tyr Phe Ser Thr Ile Leu Ser Arg Glu Tyr Asn Thr Asn
515 520 525
Phe Ile Leu Phe Pro His Ser Ser Asn Ser Gly Ser Ile Asn Glu Asn
530 535 540
Leu Ile Arg Asn Gly Asp Leu Glu Glu Gln Asp Lys Tyr Trp Glu Gly
545 550 555 560
Thr Glu Pro Ser Phe Phe Val Glu Gly Glu Gly Thr Phe Gly Ser Asn
565 570 575
Ala Leu Lys Val Gln Ala Gln Gly Ala Phe Asp Gln Ile Val Lys Leu
580 585 590
Glu Pro Asn Thr Thr Tyr Arg Leu Thr Ala Phe Thr Lys Val Lys Asp
595 600 605
Ser Asn Tyr Ser Lys Gly Arg Ile Gly Ile Arg Asp Lys Tyr Ala Tyr
610 615 620
Arg Val Glu Glu Ser Phe Leu His Thr Arg Tyr Asn Gln Thr Lys Val
625 630 635 640
Glu Phe Thr Thr Gly Ala Asp Thr Ser Lys Leu Ser Val Phe Ile Gln
645 650 655
Gly Gly Gly Asp Gln Glu Ser Ile Ala Trp Ala Asp Asn Ile Glu Leu
660 665 670
Tyr Lys Val His Gln Gln Asp Asn Met Ile Ser Asp Phe Asn Phe Asp
675 680 685
Thr Tyr Lys Tyr Ser Asn Met Cys Trp Glu Leu Ser Gly Gly Gly Asp
690 695 700
Leu Val Glu Gln Glu Gly Leu Phe Asn Ser Lys Ala Leu Lys Ile Thr
705 710 715 720
Asn Ser Gly Gly Ala Glu Gln Glu Val Lys Leu Lys Ala Asn Thr Asn
725 730 735
Tyr Ile Phe Thr Ala Tyr Val Lys Val Asp Asn Thr Thr Ala Gln Ile
740 745 750
Gly Cys Gly Ser Asn Gln Val Ser Cys Asn Ser Thr Ser Tyr Thr Pro
755 760 765
Val Lys Leu Lys Phe Arg Thr Gly Val Asp Pro Ser Thr Thr Glu Gly
770 775 780
Ser Ile Tyr Cys Ser Asn Leu Asn Asp Ser Gly Thr Val Trp Ala Asp
785 790 795 800
Asn Phe Val Leu Cys Glu Ala Pro Asn Leu Ile Ile Asn Gly Asp Phe
805 810 815
Glu Gln Phe Asp Leu Ser Ser Trp Asn Leu Ser Pro Ser Glu Gly Gly
820 825 830
Ser Ile Tyr Leu Gln Ser Gly Leu Gly Met Ser Arg Ser Asn Ala Ile
835 840 845
Val Leu Arg Gly Thr Gly Asn Glu Ala Gly Lys Ile Ser Gln Lys Val
850 855 860
Pro Leu Lys Pro Phe Thr Lys Tyr Arg Leu Thr Ala Tyr Val Lys Val
865 870 875 880
Ser Lys Gly Ser Ile Ala His Ile Gly Tyr Gly Asp Asn Thr Cys Ala
885 890 895
Cys Ala Val Asp Asp Phe Arg Gln Ala Leu Val Asp Phe Thr Thr Gly
900 905 910
Ala Asn Pro Met Gln Ser Asp Asp Ala Ile Tyr Leu Ser Ser Gly Asn
915 920 925
Ser Lys Tyr Thr Val Ala Asp Asn Phe Glu Leu Tyr Glu Leu Asp Gln
930 935 940
Ile
945
<210> 157
<211> 293
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 157
Met Asp Gln Val Asn Pro Thr Glu Lys Asn Ile Arg Val Lys Thr Asn
1 5 10 15
Thr Ser Met Gly Ile Ile Lys Trp Glu Asp Leu Ile Phe Asp Asn Leu
20 25 30
His Val Ile Gly Lys Asn Ser Gly Leu Pro Gly Lys Tyr Gln Asp Thr
35 40 45
Ile Ser Leu Ser Ser Gly Tyr Phe Trp Asp Val Glu Tyr His Gln Phe
50 55 60
Ser Val Glu Ala Asp Gly Ser Pro Ile Met Thr Asp Ser Asn Asp Thr
65 70 75 80
Phe Val Ala Lys Thr Thr Leu Thr Asn Asp Thr Asp Gln Glu Gln Thr
85 90 95
Leu Ser Thr Asn Ala Phe Ser Lys Thr Ile Thr Asn Ser Val Ser Asn
100 105 110
Thr Thr Thr His Gly Phe Lys Leu Gly Ala Lys Ala Thr Ala Lys Leu
115 120 125
Ser Val Pro Leu Ile Gly Glu Val Gly Thr Glu Leu Ser Thr Glu Tyr
130 135 140
Asn Phe Ser Asp Thr Leu Ala Arg Ser Asn Ser Gly Ser Tyr Thr Tyr
145 150 155 160
Ile Gly Ser Pro Gln Asn Ile Lys Val Pro Ala His Ser Ser Leu Glu
165 170 175
Val Ile Val Lys Leu Glu Thr Val Thr Ala Lys Gly Asn Val Arg Leu
180 185 190
Leu Thr Lys Ile Ser Gly Arg Asp Arg Gly Asn Phe Ile Tyr Thr Glu
195 200 205
Pro Glu Leu Ala Asp His Phe Asp Leu Ser Phe Asn Gln Ile Val Glu
210 215 220
Lys Ala Ser Gln Tyr Lys Asp Phe Lys Asn Ile Val Ala Asn Pro Asp
225 230 235 240
Gln Glu Thr Ile Thr Leu Ile Gly Thr Gly Glu Tyr Glu Ser Lys Tyr
245 250 255
Gly Thr Glu Phe Ser Val Thr Val Lys Pro Ile Tyr Thr Asn Asn Arg
260 265 270
Glu Lys Arg Glu Ile Asn Glu Gly Tyr Thr Tyr Lys Val Lys Pro Gln
275 280 285
Ile Ile Lys His Asp
290
<210> 158
<211> 558
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 158
Met Glu Glu Lys Lys Thr Glu Val Gln Pro Gln Gln Ala Thr Pro Asn
1 5 10 15
Ser Asn Ser Leu Gly Ala Gly Gly Gly Ser Tyr Glu Ser Asp Ala Leu
20 25 30
Lys Ala Leu Thr Asp Lys Ile Asn Met Asp Tyr Leu Lys Lys Ala Gly
35 40 45
Ser Val Ala Ala Asp Thr Leu Lys Ala Ala Tyr Lys Asp Ala His Thr
50 55 60
Ser Asn Gly Asn Tyr Asn Asn Thr Phe Arg Thr Leu Thr Met Thr Thr
65 70 75 80
Ser Ala Leu Ile Pro Tyr Gly Gly Val Leu Ile Ser Pro Ile Ile Gly
85 90 95
Leu Ile Trp Pro Glu Asp Leu Thr Ala Glu Asn Asn Lys Met Ala Ala
100 105 110
Leu Ile Lys Gln Leu Asp Glu Met Met Asp Thr Lys Val Thr Asp His
115 120 125
Val Val Arg Arg Leu Lys Thr Glu Val Asn Asp Val Lys Lys Gln Leu
130 135 140
Glu Leu Phe Glu Gln Asn Val Asn Thr Gly Lys His Lys Ser Gly Ser
145 150 155 160
Gly Leu Tyr Ser Leu Thr Glu Glu Asp Gly Asn Ala Ile Asp Ala Lys
165 170 175
Asp Ile His Lys Ala Phe Asn Ser Leu Leu Glu His Ser Lys Glu Glu
180 185 190
Met Gln Glu Val Ala Ala Leu Pro Ile Tyr Thr Ala Met Ala Thr Ala
195 200 205
His Leu Glu Phe Leu His Phe Leu Glu Gln Asn Gly Thr Lys Pro Ile
210 215 220
Ile Gly Phe Thr Ser Asn Ala Leu Asn Asn Ile Leu Lys Thr Arg Glu
225 230 235 240
Gly Leu Glu Asn Glu Thr Phe Gly Gln Lys Ile Lys Asp Leu Ala Asp
245 250 255
Lys Tyr Thr Lys His Val Gln Glu Thr Tyr Asn Ile Gly Arg Asp Lys
260 265 270
Tyr Met Ala Lys Met Gln Ala Ile Thr Thr His Ser Met Asn Glu Asn
275 280 285
Asp Glu Tyr Ala Asn Leu Ala Tyr Leu Lys Ser Leu Asp Asp Ala Arg
290 295 300
Ser Glu Met Gly Tyr Ser Gln Ser Lys Ile Asp Lys Ile Lys Asn Asp
305 310 315 320
Ile Lys Asn Tyr Gln Asn Leu Ile Lys Glu Lys Ala Asp Phe Arg Asp
325 330 335
Gln Thr Ala Glu Asn Val Ala Phe Lys Leu Thr Ala Met Gly Lys Trp
340 345 350
Asn His Gln Gly Asp Lys Trp Tyr Tyr Ile Asn Asn Gln Gly Glu Asn
355 360 365
Gln Thr Gly Trp Leu Gln Asp Gly Ser Lys Tyr Tyr Tyr Phe Ser Pro
370 375 380
Glu Lys Thr Asp Lys Phe Glu Lys Gly Gln Met Val Thr Gly Lys Val
385 390 395 400
Thr Ile Asp Lys Gly Glu Thr Tyr Tyr Phe Asn Pro His Gly Gly Gln
405 410 415
Met Gln Thr Gly Trp Ile Gln Asp Asp Gly Lys Gln Tyr Tyr Phe Ser
420 425 430
Pro Ala Asn Asn Thr Lys Asn Tyr Asp Ser Glu Ser Phe Lys Glu Gly
435 440 445
Gln Met Met Thr Gly Trp Leu Ala His Leu Asp His Tyr Tyr Tyr Met
450 455 460
Ser Pro Gly Asp Asp Lys Thr Asn Pro Tyr Gln Lys Gly Lys Thr Phe
465 470 475 480
Lys Lys Gly Glu Met Trp Thr Gly Trp Leu Glu Asn Pro Lys Lys Ala
485 490 495
Asn Glu Trp Tyr Tyr Met Thr Pro Val Ala Arg Asp Tyr Lys Val Pro
500 505 510
His Leu Ile Gly Asn Asp Asp His Ile Ile Arg Pro Val Gly Ser Met
515 520 525
Leu His Asp Glu Tyr Leu Glu Lys Gln Asp Ser Gly Asp Gly Trp Arg
530 535 540
Tyr Tyr Phe Asn Glu Ser Gly Ile Asn Thr His Arg Tyr Asn
545 550 555
<210> 159
<211> 300
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 159
Met Asp Thr Ile Pro Gln Lys Lys Leu Ile Gln Met Gln Thr Thr Tyr
1 5 10 15
Leu Met Thr Asp Gln Gln Ala Lys Asp Gln Ala Lys Gln Glu Val Ala
20 25 30
Gln Val Asn Lys Asp Leu Ser Ser Leu Val Gly Tyr Ser Val Asp His
35 40 45
Ala Ser Phe Gln Leu Ile Ser Thr Asn Ala Asn Thr Ile Glu Leu Thr
50 55 60
Ser Ala Glu Ala Thr Gly Asp Ser Asn Ile Glu Leu Gly Asn Tyr His
65 70 75 80
Asn Gly Thr Asp Leu Ile Gln Asp Tyr Lys Val Pro Gly Lys Lys Glu
85 90 95
Thr Asn Thr Ser Ser Ala Thr Ile Thr Asn Asn Glu Asp Ala Ser Ile
100 105 110
Gly Phe Glu Ser Asp Thr Glu Phe Glu Ile Gly Ile Pro Leu Phe Ala
115 120 125
Lys Gly Lys Glu Thr Leu Lys Phe Thr Thr Asn Phe Lys Tyr Ser His
130 135 140
Gly Asp Ser Arg Thr Ser Thr Thr Thr Glu Glu Val Thr Tyr Pro Glu
145 150 155 160
Gln Val Leu Lys Cys Ser Pro His Gly Thr Thr Lys Leu Val Ser Lys
165 170 175
Val Gln Lys Thr Asn Phe Thr Gly Lys Tyr Ser Gly Lys Ala Leu Ile
180 185 190
Thr Gly Val Lys Asp Ala Asp Gly Lys Ser Leu Asn Ser Glu Glu Leu
195 200 205
Phe Thr Leu Tyr Thr Leu Asn Leu Ala Val Asp Ala Ala Ala His Phe
210 215 220
Gly Asp Pro Asn Tyr Glu Gly Met Lys Ile Pro Asp Ser Leu Glu Val
225 230 235 240
Asp Trp Gln Asn His Lys Ile Tyr Ala Lys Ser Ile Thr Thr Thr Phe
245 250 255
Gln Gly Val Ala Gly His Ser Ser Asp Ala Asn Val Glu Phe Ile Pro
260 265 270
Asp Asp Lys Ala Asn Gln Ser Val Thr Met Ala Leu Ser Glu Tyr Asn
275 280 285
Arg Arg Ile Ala Ala Gly Ile Ser Leu Tyr Thr Lys
290 295 300
<210> 160
<211> 319
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 160
Met Thr Ile Val Asp Ile Glu Gln Lys Ala Tyr Glu Glu Leu Lys Trp
1 5 10 15
Tyr Ala Ile Arg Glu Leu Asn Ala Gln Ala Asn Ser Phe Thr Asn Pro
20 25 30
Ser Leu Tyr Ser Tyr Asn Ile Asn Asn Ala Asp Ala Val Pro Asp Ile
35 40 45
Val Asn Phe Lys Ser Asn Pro Gln Ile Leu Leu Thr Ser Val Gln Val
50 55 60
Leu Ser Asn Asp Thr Ser Ile Gln Gln Ser Gln Thr Leu Lys Phe Ser
65 70 75 80
Gln Lys Cys Thr Glu Thr Thr Thr Thr Ser Thr Thr Thr Gly Tyr Lys
85 90 95
Ile Gly Thr Ser Ile Lys Ser Thr Thr Lys Gly Lys Ile Lys Val Gly
100 105 110
Phe Leu Met Ala Gly Glu Ile Glu Gln Thr Val Glu Val Ser Leu Thr
115 120 125
Gly Glu Tyr Asn His Ser Thr Thr Gln Thr Thr Thr Ser Ser His Glu
130 135 140
Glu Leu Trp Glu Tyr Thr Gln Pro Val Leu Val Pro Ala Arg Ser Lys
145 150 155 160
Val Ile Ala Thr Met Tyr Ile Met Gly Gly Pro Val Thr Val Pro Met
165 170 175
Thr Leu Ser Ala Asn Ile Ala Gly Thr Gly Val Ser Pro Lys Gly Ser
180 185 190
Pro Tyr Ser Leu Ser Lys Ile Tyr Phe Lys Lys Gln Asn Ser Ser Ala
195 200 205
Asn Ser Ser Leu Ser Ala Arg Ala Ser Val Leu Tyr Asn Pro Asn Trp
210 215 220
Pro Gly Tyr Ser Pro Val Phe Thr Gly Val Gly Glu Asn Tyr Ser Leu
225 230 235 240
Asn Val Glu Gly Ala Ser Ile Ser Ser Asn Thr Val Ser Leu Tyr Ser
245 250 255
Tyr Val Glu Phe Lys Glu Tyr Pro Leu Ser Thr Asp Thr Ala Asn Leu
260 265 270
Val Ala Val Pro Met Arg Glu Tyr Tyr Ser Thr Pro Ile Leu Arg Asp
275 280 285
Gly Thr Pro Ala Leu Asn Pro Ala Asp Ser Phe Thr Pro Tyr Ser Arg
290 295 300
Phe Ala Ser Val Asp His Asn Ala His Ser Met Ser Glu Glu Glu
305 310 315
<210> 161
<211> 376
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 161
Met Ala Ile Asp Met Ser Ser Cys Thr Lys Lys Asp Tyr Asn Gln Ser
1 5 10 15
Cys Val Glu Gln Thr Asp Pro Gly Val Asn Asn Arg Thr Tyr Ala Met
20 25 30
Tyr Glu Thr Ile Asn Arg Leu Val Glu Glu Asp Thr Leu Asn Glu Gln
35 40 45
Thr Asn Trp Ser Gly Lys Ser Trp Tyr Asn Phe Ile Arg Phe Asn Tyr
50 55 60
Leu Ile Leu Pro Ser Gln Glu Gln Glu Ile Leu His Gln Leu Asn Arg
65 70 75 80
Ile Asp Pro Ser Asn Val Glu Leu Ser Glu Val Leu Arg Ala Ile Asn
85 90 95
Gly Tyr His Asn Lys Thr Asn Leu Ile Gln Asp Tyr Ala Asn Thr Leu
100 105 110
Lys Thr Thr Asp Leu Asn Ala Thr Glu Tyr Val Glu Glu Pro Thr Val
115 120 125
Asp Ser Thr Ser Leu Gln Tyr Lys Glu Leu Asn Thr Val Leu Lys Asn
130 135 140
Pro Ser Asn Glu Ala Leu Thr Lys Thr Gly Gln Ile Ile Gly Gln Lys
145 150 155 160
Ile Thr Ser Ser Thr Gln Val Thr Asn Thr His Ser Ile Asp Val Gly
165 170 175
Arg Lys Phe Ser Met Lys Asn Ser Val Asn Ile Asp Val Ala Lys Phe
180 185 190
Ile Lys Ala Gly Val Glu Ile Thr Asp Glu Ile His Ala Asn Tyr Asn
195 200 205
Tyr Thr Asn Gly Thr Thr Thr Val Asn Thr Lys Glu Asp Ser Val Thr
210 215 220
Thr Ser Pro Glu Ser Val Phe Leu Lys Pro His Thr Gly Ala Val Ile
225 230 235 240
Thr Ala Lys Leu Ser Thr Pro Ala Tyr Ile Gly Gln Leu Lys Gly Glu
245 250 255
Met Ala Leu Asp Gly Tyr Tyr Thr Asp Gln Val Asn Phe Tyr Pro Thr
260 265 270
Trp Val Gln Leu Gly Ile Tyr Asn Lys Phe Lys Val Ile Ala Met His
275 280 285
Asn Pro Asp Ile Trp Glu Lys Leu Gln Glu Leu His Phe Ser Leu Asp
290 295 300
Asp Thr Tyr Lys Lys Val Asn Phe Glu Gly Lys Leu Thr Val Ser Glu
305 310 315 320
Arg Lys Ala Pro Gly Ser Ser Phe Glu Ser Ser Thr Gln Ile Tyr Glu
325 330 335
Leu Asp Ala Lys Gly Asp Ile Asn Tyr Asn Lys Pro Val Gly Val Pro
340 345 350
Asp Thr Lys Thr Gly Glu Val Asp Ile Gln Leu Asp Met Lys Lys Gln
355 360 365
Lys Thr Leu Tyr Leu Arg Thr Asn
370 375
<210> 162
<211> 1291
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 162
Met Thr Thr Leu Asn Glu Leu Tyr Pro Ser Tyr His Asn Val Leu Ala
1 5 10 15
Ser Pro Pro Val Ile Leu Ala Asp Asp Pro Asn Ser Ala Asn Gly Phe
20 25 30
Lys Asp Met Leu Lys Lys Leu Asp Asp Thr Trp Gly Glu Tyr Asp Lys
35 40 45
Asp Phe Leu Pro Lys Pro Val Thr Ala Ser Leu Ser Ser Leu Ile Asp
50 55 60
Leu Phe Asn Lys Lys Pro Gly Ser Ile Val Gln Phe Ile Gln Thr Gly
65 70 75 80
Ile Arg Leu Ala Phe Leu Ala Val Pro Gly Gly Gln Thr Tyr Ala Trp
85 90 95
Ala Leu Asn Lys Ile Ile Gly Leu Cys Phe Pro Thr Ser Gln Pro Asn
100 105 110
Leu Phe Glu Gln Ile Lys Glu Ala Val Gln Gln Leu Ile Asp Thr Ala
115 120 125
Leu Val Gln Thr Glu Ile Asn Lys Asn Val Asn Ala Leu Asn Gly Met
130 135 140
Thr Leu Pro Ile Arg Tyr Val Ser Asp Ala Met Gln Gln Ala Ile Gly
145 150 155 160
Lys Pro Thr Phe Ser Ser Ala Tyr Ala Gly Thr Asp Gly Cys Ser Thr
165 170 175
Asp Ala Ser Phe Ser Ser Thr Val Tyr Ala Ser Ser Cys Asp Ser Thr
180 185 190
Glu Cys Ser Cys Ser Asp Ala Pro Asp Arg Asn Phe Cys Thr Ala Asp
195 200 205
Pro Cys Pro Ser Pro Cys Lys Cys Arg Leu Asp Ala Val Tyr Asn Gln
210 215 220
Phe Asp Ala Ser Arg Lys Thr Val Glu Asn Ile Leu Thr Gly Met Arg
225 230 235 240
Thr Asn Leu Thr Thr Val Leu Lys Pro Glu Glu Met Thr Gln Tyr Met
245 250 255
Gln Ala Phe Leu Pro Leu Tyr Val Ala Gly Ala Thr Ile Met Leu Ser
260 265 270
Leu Tyr Arg Ser Tyr Ile Ala Phe Ala Gln Arg Tyr Glu Tyr Ala Leu
275 280 285
Gly Pro Asn Asp Thr Thr Lys Thr Thr Asn Asp Gln Leu Arg Val Lys
290 295 300
Ile Ser Glu Glu Ser Lys Tyr Val Tyr Glu Gln Phe Ser Lys Tyr Leu
305 310 315 320
Pro Ala Val Asp Ser Asn Thr Lys Gly Lys Leu Asn Ala Tyr Ile Gln
325 330 335
Tyr Thr Arg Asn Ile Thr Ile Asn Ala Leu Asp Gln Val Ser Thr Trp
340 345 350
Pro Phe Phe Asp Thr Trp Asp Tyr Pro Ser Ser Asp Lys Ala Gly Thr
355 360 365
Leu Ser Arg Pro Leu Ser Leu Ala Phe Asn Pro Thr Arg Ile Ile Phe
370 375 380
Asn Asp Ile Val Gly Pro Val Glu Cys Lys Thr Gly Ser Gln Asp Ser
385 390 395 400
Ser Leu Leu Asn Leu Asn Pro Leu Tyr Asp Ile Asp Gly Lys Ala Leu
405 410 415
Leu Ile Asn Thr Ile Thr Asn Tyr Phe Tyr Asp Asn Ile Gln Leu Lys
420 425 430
Ser Leu Asn Phe Gln Thr Tyr Asn His Asp Asp Asn Ile Tyr His Gln
435 440 445
His Phe Pro Leu Gly Phe Gln Ala Thr Tyr Ser Thr Arg Asp Asn Ser
450 455 460
Thr Leu Lys Val Asn His Thr Val Pro Asp Gly Ser Asn Ile Ser Leu
465 470 475 480
Gly Asn Asp Ile Phe Gly Ser Tyr Pro Ala Phe Asn Ile Ile Asn Met
485 490 495
Ser Ser Ser Gln Leu Asn Ala Gly Leu Ala Tyr Ser Asp Val Ser Asn
500 505 510
Ile Ser Phe Asp Thr Phe Ile Pro Ala Gly Cys Ser Ser Gln Asn Lys
515 520 525
Val Ser Tyr Pro Asp His Arg Ile Gln Ala Ile Tyr Pro Val Ile Pro
530 535 540
Asp Ser Ala Ser Arg Lys Lys Tyr Asp Ser Tyr Gly Asn Ser Asp Lys
545 550 555 560
Pro Gly Phe Ile Thr Thr Leu Ile Pro Asn Asp Thr Thr Thr Trp Ile
565 570 575
Thr Phe Thr Asn Leu Gly Ile Asn Thr Phe Ser Ala Glu Gln Thr Thr
580 585 590
Asn Thr Ser Gly Thr Lys Leu Val Glu Tyr Met Asn Gly Ala Ala Ala
595 600 605
Leu Lys Leu Gln Pro Gly Gln Phe Ala Gln Tyr Ser Ile Asn Thr Ser
610 615 620
Leu Phe Val Leu Pro Ser Asp Phe Lys Tyr Gln Val Arg Val Arg Ala
625 630 635 640
Ala Thr Thr Ala Thr Gly Ser Leu Ser Ile Gln Val Gly Glu Thr Pro
645 650 655
Ala Gln Thr Leu Gln Leu Pro Lys Thr Glu Asn Gln Pro Gly Gly Ile
660 665 670
Val Gly Lys Gln Gly Thr Tyr Met Pro Ile Pro Ala Ser His Ser Met
675 680 685
Asp Leu Thr Gln Arg Asp Thr Asn Leu Phe Thr Val Pro Ser Thr Ser
690 695 700
Ser Leu Ser Met Ser Ile Thr Asn Asn Ser Ala Thr Asp Ile Ile Leu
705 710 715 720
Asp Arg Ile Glu Phe Ile Pro Leu Gln Ala Ile Thr Pro Ser Asn Met
725 730 735
Ser Pro Arg Gln Phe Gly Pro Ala Ser Thr Glu Gln Thr Glu Thr Val
740 745 750
Ala Ile Arg Met Asp Val Tyr Tyr Gly Ile Lys Asp Ile Gln Ala Ile
755 760 765
Val Ser Ser Gly Gly Tyr Ala Lys Phe Arg Tyr Ser Arg His Ser Arg
770 775 780
Arg Gly Ser Phe Ile Val Tyr Glu Ser Thr Thr Asp Ser Asn Thr Trp
785 790 795 800
Asp Tyr Asp Gly Ser Phe Asp Thr Met Asp Ile Ile Tyr Leu Gly Thr
805 810 815
Val Asn Phe Leu Gly Gly Leu Ile Asp Tyr Tyr His Pro Ser Thr Ile
820 825 830
Glu Asp Leu Glu Asn Val Thr Glu Gln Val Tyr Ala Leu Phe Thr Ser
835 840 845
Ser Ser Ser Thr Glu Leu Ala Pro Thr Val Ser Ser Tyr Gln Ile Asn
850 855 860
Gln Val Ala Met Lys Val Arg Ala Leu Ser Asp Glu Val Phe Cys Lys
865 870 875 880
Glu Lys Ala Leu Leu Arg Lys Leu Val Asn Lys Ala Lys Gln Phe Leu
885 890 895
Lys Ala Arg Asn Leu Leu Val Gly Gly Asp Phe Glu Thr Leu Asn Lys
900 905 910
Trp Leu Leu Gly Thr Gln Ala Ile Ile Asn Asp Asp Ser Pro Leu Phe
915 920 925
Asn Gly Asp Tyr Leu Phe Leu Gln Pro Thr Asn Gly Ile Tyr Ser Ser
930 935 940
Tyr Ala Tyr Gln Lys Val Asp Glu Ser Lys Leu Lys Pro Tyr Thr Arg
945 950 955 960
Tyr Lys Val Ser Gly Phe Ile Gly Gln Ser Asn Arg Val Glu Leu Val
965 970 975
Val Ser Arg Tyr Gly Lys Glu Ile Asp Thr Ile Leu Asn Val Pro Tyr
980 985 990
Ala Ala Ala Leu Pro Ile Thr Ser Glu Pro Asn Pro Ser Cys Asp Asn
995 1000 1005
Ser Gln Pro Asp Ser His Phe Phe Ser Tyr Ser Ile Asp Val Gly
1010 1015 1020
Pro Leu His Pro Glu Leu Asn Pro Gly Ile Glu Phe Gly Leu Arg
1025 1030 1035
Ile Val Gln Pro Asn Gly Tyr Ala Thr Val Ser Asn Leu Glu Ile
1040 1045 1050
Met Glu Glu Arg Pro Leu Thr Asp Lys Glu Ile Gln Thr Ile Asn
1055 1060 1065
Arg Lys Asp Gln Lys Trp Glu Lys Ser Val Glu Gln Glu Arg Ala
1070 1075 1080
Lys Ile Ser Ala Leu Leu Gln Pro Thr Ile Asp Gln Ile Asn Ser
1085 1090 1095
Phe Phe Lys Asp Ala Asp Trp Asn Gly Thr Ile Leu Pro His Val
1100 1105 1110
Thr Tyr Gln Ser Leu Ala Ser Val Val Leu Pro Ala Leu Pro Lys
1115 1120 1125
Leu Arg His Trp Phe Met Asn Asp Arg Ala Gly Glu His Tyr His
1130 1135 1140
Ile Leu Gln Glu Leu Lys His Ala Leu Asp Tyr Val Phe Thr Gln
1145 1150 1155
Leu Glu Glu Gln Asn Leu Ile His Asn Gly Asn Phe Thr Asn Gly
1160 1165 1170
Leu Thr Asp Trp Leu Val Glu Gly Asp Ala Gln Ile Thr Leu Leu
1175 1180 1185
Ala Asn Asn Lys Pro Ala Leu Gln Leu Ser Asp Trp Asp Ala Ser
1190 1195 1200
Ala Ser Gln Ser Leu Asp Ile Ser Asp Phe Asp Glu Asn Lys Gln
1205 1210 1215
Tyr Lys Leu Arg Val Tyr Ala Lys Gly Thr Gly Thr Ile Thr Val
1220 1225 1230
Glu Asn Cys Glu Glu Ser Asn Pro Val Ser Phe Asp Thr Asp Thr
1235 1240 1245
Phe Thr Ser Gln Glu Gln Ile Phe Tyr Phe Asp Thr Pro Ser Phe
1250 1255 1260
Val Leu Arg Met Gln Ser Glu Gly Thr Glu Phe Val Val Gly Ser
1265 1270 1275
Val Glu Leu Ile Glu Leu Ser Asp Glu Asp Thr Asn Glu
1280 1285 1290
<210> 163
<211> 728
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 163
Met Thr Thr Leu Asn Glu Leu Tyr Pro Ser Tyr His Asn Val Leu Ala
1 5 10 15
Ser Pro Pro Val Ile Leu Ala Asp Asp Pro Asn Ser Ala Asn Gly Phe
20 25 30
Lys Asp Met Leu Lys Lys Leu Asp Asp Thr Trp Gly Glu Tyr Asp Lys
35 40 45
Asp Phe Leu Pro Lys Pro Val Thr Ala Ser Leu Ser Ser Leu Ile Asp
50 55 60
Leu Phe Asn Lys Lys Pro Gly Ser Ile Val Gln Phe Ile Gln Thr Gly
65 70 75 80
Ile Arg Leu Ala Phe Leu Ala Val Pro Gly Gly Gln Thr Tyr Ala Trp
85 90 95
Ala Leu Asn Lys Ile Ile Gly Leu Cys Phe Pro Thr Ser Gln Pro Asn
100 105 110
Leu Phe Glu Gln Ile Lys Glu Ala Val Gln Gln Leu Ile Asp Thr Ala
115 120 125
Leu Val Gln Thr Glu Ile Asn Lys Asn Val Asn Ala Leu Asn Gly Met
130 135 140
Thr Leu Pro Ile Arg Tyr Val Ser Asp Ala Met Gln Gln Ala Ile Gly
145 150 155 160
Lys Pro Thr Phe Ser Ser Ala Tyr Ala Gly Thr Asp Gly Cys Ser Thr
165 170 175
Asp Ala Ser Phe Ser Ser Thr Val Tyr Ala Ser Ser Cys Asp Ser Thr
180 185 190
Glu Cys Ser Cys Ser Asp Ala Pro Asp Arg Asn Phe Cys Thr Ala Asp
195 200 205
Pro Cys Pro Ser Pro Cys Lys Cys Arg Leu Asp Ala Val Tyr Asn Gln
210 215 220
Phe Asp Ala Ser Arg Lys Thr Val Glu Asn Ile Leu Thr Gly Met Arg
225 230 235 240
Thr Asn Leu Thr Thr Val Leu Lys Pro Glu Glu Met Thr Gln Tyr Met
245 250 255
Gln Ala Phe Leu Pro Leu Tyr Val Ala Gly Ala Thr Ile Met Leu Ser
260 265 270
Leu Tyr Arg Ser Tyr Ile Ala Phe Ala Gln Arg Tyr Glu Tyr Ala Leu
275 280 285
Gly Pro Asn Asp Thr Thr Lys Thr Thr Asn Asp Gln Leu Arg Val Lys
290 295 300
Ile Ser Glu Glu Ser Lys Tyr Val Tyr Glu Gln Phe Ser Lys Tyr Leu
305 310 315 320
Pro Ala Val Asp Ser Asn Thr Lys Gly Lys Leu Asn Ala Tyr Ile Gln
325 330 335
Tyr Thr Arg Asn Ile Thr Ile Asn Ala Leu Asp Gln Val Ser Thr Trp
340 345 350
Pro Phe Phe Asp Thr Trp Asp Tyr Pro Ser Ser Asp Lys Ala Gly Thr
355 360 365
Leu Ser Arg Pro Leu Ser Leu Ala Phe Asn Pro Thr Arg Ile Ile Phe
370 375 380
Asn Asp Ile Val Gly Pro Val Glu Cys Lys Thr Gly Ser Gln Asp Ser
385 390 395 400
Ser Leu Leu Asn Leu Asn Pro Leu Tyr Asp Ile Asp Gly Lys Ala Leu
405 410 415
Leu Ile Asn Thr Ile Thr Asn Tyr Phe Tyr Asp Asn Ile Gln Leu Lys
420 425 430
Ser Leu Asn Phe Gln Thr Tyr Asn His Asp Asp Asn Ile Tyr His Gln
435 440 445
His Phe Pro Leu Gly Phe Gln Ala Thr Tyr Ser Thr Arg Asp Asn Ser
450 455 460
Thr Leu Lys Val Asn His Thr Val Pro Asp Gly Ser Asn Ile Ser Leu
465 470 475 480
Gly Asn Asp Ile Phe Gly Ser Tyr Pro Ala Phe Asn Ile Ile Asn Met
485 490 495
Ser Ser Ser Gln Leu Asn Ala Gly Leu Ala Tyr Ser Asp Val Ser Asn
500 505 510
Ile Ser Phe Asp Thr Phe Ile Pro Ala Gly Cys Ser Ser Gln Asn Lys
515 520 525
Val Ser Tyr Pro Asp His Arg Ile Gln Ala Ile Tyr Pro Val Ile Pro
530 535 540
Asp Ser Ala Ser Arg Lys Lys Tyr Asp Ser Tyr Gly Asn Ser Asp Lys
545 550 555 560
Pro Gly Phe Ile Thr Thr Leu Ile Pro Asn Asp Thr Thr Thr Trp Ile
565 570 575
Thr Phe Thr Asn Leu Gly Ile Asn Thr Phe Ser Ala Glu Gln Thr Thr
580 585 590
Asn Thr Ser Gly Thr Lys Leu Val Glu Tyr Met Asn Gly Ala Ala Ala
595 600 605
Leu Lys Leu Gln Pro Gly Gln Phe Ala Gln Tyr Ser Ile Asn Thr Ser
610 615 620
Leu Phe Val Leu Pro Ser Asp Phe Lys Tyr Gln Val Arg Val Arg Ala
625 630 635 640
Ala Thr Thr Ala Thr Gly Ser Leu Ser Ile Gln Val Gly Glu Thr Pro
645 650 655
Ala Gln Thr Leu Gln Leu Pro Lys Thr Glu Asn Gln Pro Gly Gly Ile
660 665 670
Val Gly Lys Gln Gly Thr Tyr Met Pro Ile Pro Ala Ser His Ser Met
675 680 685
Asp Leu Thr Gln Arg Asp Thr Asn Leu Phe Thr Val Pro Ser Thr Ser
690 695 700
Ser Leu Ser Met Ser Ile Thr Asn Asn Ser Ala Thr Asp Ile Ile Leu
705 710 715 720
Asp Arg Ile Glu Phe Ile Pro Leu
725
<210> 164
<211> 667
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 164
Met Asn Ser Tyr Asn Lys Asn Glu Tyr Glu Ile Val Asp Ser Pro Tyr
1 5 10 15
Ser Ser Asn Asn Ser Asn Leu Tyr Thr Asn Ser Arg Tyr Pro Tyr Val
20 25 30
Asn Asn Pro Ser Gln Phe Met Gln Asn Thr Thr Tyr Glu Asp Trp Leu
35 40 45
Asn Leu Cys Lys Asp Asn Ser Lys Tyr Asn Gln Asp Thr Asn Gly Phe
50 55 60
Ile Asp Thr Pro Asp Thr Lys Thr Ala Val Thr Ala Ala Ile Thr Val
65 70 75 80
Val Gly Ala Ile Leu Thr Tyr Ala Gly Val Pro Gly Ala Gly Ala Phe
85 90 95
Thr Leu Leu Gly Lys Val Val Pro Val Phe Trp Pro Ser Ala Ser Asp
100 105 110
Lys Ser Gln Asn Ile Trp Val Lys Phe Ile Glu Ser Ile Gln Asp Ile
115 120 125
Leu Gly Lys Lys Leu Asp Ile Asn Lys Arg Asn Lys Ala Ile Asp Lys
130 135 140
Leu Glu Gly Leu Gln Ser Val Ile Tyr Asn Tyr Asn Ala Ser Leu Lys
145 150 155 160
Ser Trp Leu Glu Asn Lys Asn Asn Ser Ser Phe Gln Thr Asp Val Val
165 170 175
Lys Gly Asn Phe Ile Tyr Ala Asn Asn Ala Phe Ile Glu Ser Met Asn
180 185 190
Val Phe Thr Ser Ser Ser Gly Asn Ser Lys Glu Lys Glu Glu Asn Val
195 200 205
Leu Leu Leu Pro Ile Tyr Thr His Ile Ala Asn Leu His Leu Leu Leu
210 215 220
Leu Arg Asp Ala Ser Phe Tyr Gly Lys Tyr Trp Gly Phe Ser Gly Ala
225 230 235 240
Glu Ile Asn Asp Phe Tyr Ser Lys Gln Gln Gln Asp Thr Ile Thr His
245 250 255
Ile Ala His Cys Ile Lys Tyr Tyr Asn Glu Gly Leu Thr Ile Ala Lys
260 265 270
Glu Ala Phe Gln Leu Glu Asp Lys Trp Asn Lys Phe Asn Ser Tyr Arg
275 280 285
Arg Asn Met Thr Leu Lys Val Leu Asp Val Ile Ser Leu Phe Ser Thr
290 295 300
Tyr Asp Thr Lys Arg Tyr Pro Ala Thr Ile Glu Lys Pro Asn Ile Asn
305 310 315 320
Glu Leu Gly Lys Phe Glu Leu Thr Arg Glu Val Tyr Thr Asp Ala Phe
325 330 335
Ser Asp Pro Gly Ser Ser Leu Asn Ser Ser Leu Thr Met Asp Glu Leu
340 345 350
Asp Lys Lys Phe Thr Arg Gly Pro His Leu Phe Asp Gln Leu Ile Lys
355 360 365
Leu Phe Leu Tyr Thr Arg Phe Ser His Asp Thr Phe Gly Phe Thr Gly
370 375 380
Asn Lys Asn Cys Thr Ala Leu Ile Asn Asn Asn Phe Cys Gln Glu Ala
385 390 395 400
Gln Tyr Gly Asp Ile Gln Lys Ala Asp Thr Glu Ser Thr Phe Ser Ile
405 410 415
Asn Gln Leu Ile Asn Lys Ile Asn Val Thr His Val Arg Asn Asn Pro
420 425 430
Gly Ala Ile Ile Arg Met Asp Phe Tyr Lys Glu Gln Asn Leu Leu Phe
435 440 445
Gln Tyr Asn Ser Gly Ser Lys Ala Thr Leu Val Asp Val Lys Thr Val
450 455 460
Gln Ile Ser Asn Gln Ile Asp Ser Ser Ile Pro Asn Ser Glu Thr Tyr
465 470 475 480
Lys His Asn Phe Ser Tyr Leu Lys Thr Gly Asn Asn Pro Thr Asn Pro
485 490 495
Asn Leu Lys Ile Arg Gln Val Ala Phe Ala Trp Thr His Trp Ser Val
500 505 510
Asp Ser Asn Asn Thr Ile Gln Glu Lys Val Ile Thr Ala Ile Pro Ile
515 520 525
Ile Lys Ser Gln Ser Val Asn Asp Thr Thr Ile Ile Pro Asn Pro Gly
530 535 540
His Leu Gly Gly Asp Phe Ile Gln Phe Asn Pro Gly Thr Ser Pro Arg
545 550 555 560
Ile Glu Leu Gln Cys Arg Ala Ser Asn Phe Pro Thr Thr Lys Asn Tyr
565 570 575
Ala Val Arg Ile Arg Tyr Val Ala Asn Gln Lys Phe Thr Val Ser Val
580 585 590
Ser Ile Ala Gly Arg Ala Thr Thr Phe Ile Thr Ala Asp Thr Met Ala
595 600 605
Ala Ser Thr Gln Gly Lys Tyr Leu Tyr Gln Gln Phe Ser Tyr Gly Asp
610 615 620
Val Phe Thr Lys Ser Leu Pro Val Pro Leu Ser Pro Arg Gln Leu Leu
625 630 635 640
Thr Ile Ser Ile Thr Thr Leu Ser Pro Ile Asn Gln Asn Thr Ile Phe
645 650 655
Ala Ile Asp Arg Ile Glu Phe Ile Pro Val Asp
660 665
<210> 165
<211> 376
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 165
Met Ala Glu Asn Thr Val Gln Thr Ile Gln Val Ser Asn Met Lys Lys
1 5 10 15
Asn Met Thr Ser Tyr Lys Asp Phe Glu Tyr Gln Leu Gly Pro Glu Gly
20 25 30
Leu Arg Glu Ala Met Ala Thr Thr Gly Ser Asn Ala Leu Val Met Asp
35 40 45
Leu Tyr Ala Leu Thr Leu Ile Lys Gln Gln Asn Ala Asp Phe Thr Asn
50 55 60
Val Asp Ile Ile Asp Glu Lys Leu Arg Ala Ala Ile Thr Thr His Gln
65 70 75 80
Asn Val Gly Arg Ser Asn Ala Thr Lys Trp Leu Asp Met Leu Lys Pro
85 90 95
Lys Leu Ile Thr Thr Asn Gln Asn Ile Ile Asn Tyr Asn Thr Lys Phe
100 105 110
Gln Asn Tyr Tyr Asp Thr Leu Leu Asp Ala Val Asp Ala Thr Asp Lys
115 120 125
Ala Thr Leu Lys Lys Gly Leu Ser Asn Leu Asn Thr Ser Ile Ser Ile
130 135 140
Tyr Lys Lys Asp Val Asp Asn Leu Ile Ala Glu Leu Ile Ser Phe Arg
145 150 155 160
Asp Arg Met Ala Thr Asp Thr Gln Asn Phe Lys Ala Asp Thr Ser Ser
165 170 175
Leu Val Ser Ile Leu Thr Ser Glu Asp Ser Gly Leu Pro Ile Leu Gln
180 185 190
Gln Gln Ile Asp Ala Tyr Asn Ala Ala Ile Ala Lys Asp Asn Ala Ile
195 200 205
Ile Ile Gly Gly Ser Val Ala Leu Gly Leu Gly Ala Val Gly Ile Ile
210 215 220
Gly Gly Gly Ile Leu Ile Gly Thr Gly Ala Gly Ser Gly Leu Gly Val
225 230 235 240
Thr Leu Val Val Gly Gly Val Ser Ala Ile Ala Gly Gly Thr Thr Ala
245 250 255
Ile Val Leu Ala Lys Gln Asn Ile Asp Ser Ser Arg Glu Gly Ile Arg
260 265 270
Asp Leu Thr Leu Asn Leu Asn Asp Thr Lys Ala Gln Met Val Val Val
275 280 285
Asn Asn Ile Lys Asn Gln Cys Ile Tyr Leu Ser Asp Thr Ile Asp Leu
290 295 300
Ala Ile Val Ala Leu Gln Asn Ile Ser Asn Gln Trp His Ser Met Ser
305 310 315 320
Ala Arg Tyr Thr Thr Leu Ile Glu Asn Val Asp Ser Ile Asn Pro Asn
325 330 335
Glu Phe Ala Phe Ile Lys Glu Asp Leu His Val Ala Ser Asp Ser Trp
340 345 350
Lys Asp Ile Lys Glu Tyr Ala Asp Lys Leu Tyr Thr Asp Ile Lys Val
355 360 365
Val Glu Thr Asp Gly Pro Glu Leu
370 375
<210> 166
<211> 309
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 166
Met Asp Ser Asn Asn Ile Val Asn Lys Val Pro Glu Gln Gln Gln Pro
1 5 10 15
Leu Lys Ser Gln Asp Pro Val Ser Val Pro Ser Glu Asp Val Lys Gly
20 25 30
Leu Gln Asp Leu Trp Gln Arg Lys Asp Ile Lys Thr Ala Leu Ala Met
35 40 45
Val Ala Ser Val Ser Phe Asp Asp Leu Arg Ser Ile Gly Gly Phe Asn
50 55 60
Phe Leu Tyr Tyr Gln Lys Pro Ser Tyr Ser Val Glu Leu Ile Gly Asn
65 70 75 80
Pro Lys Asn Glu Pro Ser Ser Pro Val Tyr Leu Gly Thr Ser Asn Leu
85 90 95
Ser His Ser Gly Gly Thr Ser Glu Gln Thr Leu Ser Ser Ala Ala Phe
100 105 110
Ser Lys Ala Val Thr Ser Thr Val Ser Ala Thr Thr Thr His Gly Ala
115 120 125
Lys Val Gly Ala Lys Ala Gly Ala Lys Leu Gln Val Pro Leu Val Ala
130 135 140
Glu Ala Ser Val Glu Leu Asn Ala Glu Tyr Asn Phe Ala Asn Ala Gly
145 150 155 160
Thr Glu Thr Asn Thr Glu Thr Val Thr Tyr Thr Val Pro Ser Gln Asn
165 170 175
Val Lys Leu Lys Pro Gly Glu Thr Ala Lys Val Thr Ala Ser Leu Arg
180 185 190
Met Ile Glu Ala Ser Gly Asp Val Asn Leu Arg Thr Thr Tyr Thr Gly
195 200 205
Lys Val Glu Ser Asn Tyr Glu Arg Tyr Ile Gly His Gly Met Gly Glu
210 215 220
Ile Ser Asp Thr Asn Lys Ser Gly Leu Gly Ser Trp Ile Gln Ala Leu
225 230 235 240
Ala Phe Leu Asp Ser Asp Leu Asn Asn Tyr Trp Ser Tyr Ser Asn Ser
245 250 255
Asp His Asp Val Val Tyr Gln Asp Gly Lys Gly Lys Phe Lys Ala Thr
260 265 270
Tyr Gly Thr Leu Val Asp Ile Lys Val Glu Ile Ser Lys Glu Thr Asp
275 280 285
Ser Lys Asp Asn Thr Pro Ile Arg Ser Tyr Ser Tyr Thr Val Thr Pro
290 295 300
Glu Ile Thr Lys Lys
305
<210> 167
<211> 292
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 167
Met Ala Glu Ile Asn Ser Ile Gln Ser Ala Ala Gln Glu Gln Gly Ser
1 5 10 15
Val Ser Ile Ser Asp Trp Arg Glu Pro Phe Arg Asn Ala Tyr Lys Ile
20 25 30
Ala Gln Lys His Asp Pro Tyr Thr Tyr Phe Gly Glu Asp Ala Thr Glu
35 40 45
Tyr Thr Leu Gly Asn Val Tyr Gln Ser Ser Val Glu Ala Asp Gly Ser
50 55 60
Pro Thr Ile Thr Gly Ser Lys Ser Leu Phe Ala Gly Arg Thr Thr Leu
65 70 75 80
Thr Asn Asp Ser Asp Gln Glu Gln Thr Leu Thr Thr Asp Gln Phe Ser
85 90 95
Lys Thr Phe Glu Asn Ser Val Thr Asn Ser Thr Thr Asn Gly Phe Asn
100 105 110
Leu Gly Val Ser Ala Ser Ser Ser Phe Gly Ile Pro Leu Ile Gly Glu
115 120 125
Thr Ser Val Glu Ile Ser Thr Glu Tyr Asn Phe Ser Ser Thr Glu Glu
130 135 140
Lys Thr Lys Ser Glu Ser Tyr Thr Tyr Thr Ala Ser Pro Gln Asn Ile
145 150 155 160
Lys Val Pro Ala His Ser Ser Val Glu Val Ile Val Asn Leu Asn Thr
165 170 175
Ala Lys Ile Ser Gly Asn Val Lys Leu Leu Ser Lys Ile Asp Gly Thr
180 185 190
Phe Thr His Ala Ser Gly Gly Asp Gly Ser Asn Thr Ala Ser Arg Gly
195 200 205
Leu Trp Val Leu Glu Tyr Asp Ala Ser Pro Tyr Glu Lys Leu Ser Asp
210 215 220
Leu Lys Ser Ala Arg Val Gly Ala Glu Ala Tyr Gly Pro Val Tyr Leu
225 230 235 240
Val Gly Ser Gly Lys Tyr Ser Ala Glu Tyr Gly Thr Glu Phe Ala Val
245 250 255
Thr Val Arg Pro Val Glu Lys Pro Asn Gly Phe Lys Ser Met Ile Ala
260 265 270
Glu Asn Ser Lys Ser Thr Asn Glu Gly Tyr Thr Tyr Lys Val Lys Pro
275 280 285
Glu Ile Lys Lys
290
<210> 168
<211> 541
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 168
Met Pro Ile Leu Trp Pro Asp Asp Glu Glu Pro Gln Lys Val Trp Glu
1 5 10 15
Asp Leu Ile Asn His Gly Asp Lys Phe Phe Asp Glu Lys Ile Gly Lys
20 25 30
Ile Glu Lys Ala Arg Ala Lys Thr Phe Ile Ala Gly Met Lys Asn Glu
35 40 45
Leu Gly Lys Tyr Glu Lys Ala Leu Glu Asp Trp Lys Ala Asn Lys Asn
50 55 60
Gln Asp Thr Ala Ala Thr Val Gln Glu Glu Phe Asp Asp Ala Gly Asp
65 70 75 80
Tyr Leu Asp Thr Lys Met Glu Glu Leu Lys Ile Glu Gly Tyr Glu Gln
85 90 95
Thr Leu Leu Ser Cys Tyr Ala Gln Ala Ala Leu Met His Leu Asn Leu
100 105 110
Leu Ser Gln Gly Val Gln Tyr Ala Asp Glu Trp Arg Lys Asn Asn Pro
115 120 125
Gly Gln Thr Ser Lys Tyr Trp Arg Glu Met Leu Thr Lys Gly Asp Lys
130 135 140
Asn Arg Arg Gly Arg Ile Glu Ala Tyr Met Asp Tyr Cys Thr Lys Thr
145 150 155 160
Tyr Arg Glu Gly Leu Asn Lys Val Lys Asn Val Pro Asn Ile Thr Trp
165 170 175
Tyr Thr Tyr Asn Ser Tyr Arg Arg Glu Met Thr Leu Ala Val Leu Asp
180 185 190
Leu Ile Ala Leu Phe Pro Asn Tyr Asp Val Asp Arg Tyr Pro Lys Gly
195 200 205
Thr Lys Ser Glu Leu Thr Arg Glu Ile Tyr Met Asp Asn Gly Tyr Thr
210 215 220
Lys Asp Val Asp Val Gly Asp Leu Pro Glu Leu Asp Gln Ala Leu Thr
225 230 235 240
Arg Arg Pro His Leu Phe Asp Ser Leu Ala Glu Leu Lys Leu Ile Pro
245 250 255
Ser Asn Val Gly Leu Ala His Cys Leu Ile Lys Ser Arg Arg Ile Asn
260 265 270
Asn Asp Val Leu Val Gly Tyr Asp Leu Pro Gly Ser Arg His Leu Ser
275 280 285
Ser Leu Pro Ile Ile Gly Val Asn Val Glu Trp Arg Glu Lys His Phe
290 295 300
His Gly Gly Lys Leu Cys Asp Arg Ala Asn Thr Gly Ile Glu Ile Leu
305 310 315 320
Thr Trp Ile Pro Asp Phe Gly Val Thr Gln Ser Ser Ile Phe Lys Met
325 330 335
Glu Asp Gly Gly Gly Cys Ile Glu His Gly Asn Gly Ile Gln Pro Pro
340 345 350
Arg Lys Asn Ala Lys Arg Asp Pro Leu Gly Gly Tyr Ser His Arg Leu
355 360 365
Ser Tyr Met Ser Val Arg Glu Ala Gln Asn Pro Ser Phe Tyr Met Tyr
370 375 380
Ser Tyr Ala Trp Thr His Leu Ser Val Asp Glu Lys Asn Thr Ile Ala
385 390 395 400
Lys Asp Thr Ile Thr Gln Ile Pro Ala Val Lys Ala Val Glu Gln Asn
405 410 415
Asn Asp Phe Val Val Ile Ala Gly Pro Gly His Thr Gly Gly Asp Leu
420 425 430
Val Lys Leu Asn Gly Ile Thr Lys Gln Gly Leu Lys Leu Lys Cys Val
435 440 445
Phe Pro Gln Asn Lys Lys Tyr Lys Ile Arg Leu Arg Tyr Ala Ala Asp
450 455 460
Asn Asp Thr Arg Val Asn Ile Ala Val Pro Asp Ser Asp Gly Thr Thr
465 470 475 480
Ala Tyr Trp Ile Asp Ala Lys Lys Thr Tyr Pro Gly Val Glu Leu Lys
485 490 495
Tyr Asn Asn Phe Lys Tyr Leu Asp Thr Asp Ala Ile Val Asn Thr Pro
500 505 510
Asn Lys Ala Val Thr Ile Ala Ile Lys Asn Ala Leu Lys Asp Thr Ser
515 520 525
Ile Ile Leu Asp Arg Ile Glu Phe Ile Pro Gln Glu Gly
530 535 540
<210> 169
<211> 216
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 169
Met Tyr Asn Lys Arg Ile Ile Asp Leu Glu Ile Pro Ser Tyr Asn Ala
1 5 10 15
Ile Asn Phe Leu Glu Ile Tyr Arg Leu Pro Leu Val Tyr Leu Asn Gln
20 25 30
Ala Leu Asn Ile Thr Gln His Leu Gln Lys Ser Leu Ser Leu Gln Thr
35 40 45
Leu Asn Leu Glu Phe Asp Gln Leu Asn Asn Leu Ile Ser Glu Ile Pro
50 55 60
Gly Gly Ser Ile Leu Asn Thr Ile Asn Gln Lys Ile Glu Tyr Ser Glu
65 70 75 80
Ile Asn Thr Lys Cys Ala Ile Asn Asn Ile Val Asp Ile Val Lys Ser
85 90 95
Asn Cys Glu Met Phe Glu Asp Asp Phe Ser Leu Gln Ser Lys Leu Glu
100 105 110
Ser Val Ile Ser Asn Val Phe Leu Asn Leu Ala Asn Gln Glu Glu Asn
115 120 125
Pro Tyr Val Phe Tyr His Glu Lys Leu Ser Tyr Asn Thr Ser Tyr Ser
130 135 140
Tyr His Val Ile Phe Ile Ile Asp Asn Met Glu Ser Asn Leu Asn Val
145 150 155 160
Phe Pro Ile Ser Phe Asn Val Asn Val Asp Ser Asn Tyr Glu Asn Leu
165 170 175
Asn Arg Asn Ser Ile Lys Asp Leu His Ser Tyr Lys Ile Lys Ile Ser
180 185 190
Gly Ile Asn Phe Val Ile Ser Lys Asn Ser Glu Leu Ala Glu Met Val
195 200 205
Glu Arg Val Thr Glu Ser Ile Leu
210 215
<210> 170
<211> 332
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 170
Met Glu Gln Asn Ile Gln Gly Gln Gln Ser Asn Glu Lys Tyr Ala Leu
1 5 10 15
Gly Ala Glu Gly Phe Lys Glu Glu Val Gly His Ser Gly Ser Ser Ile
20 25 30
Leu Val Met Asp Ser Tyr Ala Lys Ile Ile Phe Asn Asn Ser Gln Glu
35 40 45
Ile Asp Phe Asn Gly Val Asn Ser Ile Ser Asn Glu Leu Lys Thr Asn
50 55 60
Ile Val Ser His Gln Arg Ala Ala Arg Asn Asn Ala Ala Tyr Trp Leu
65 70 75 80
Glu Asn Ile Arg Pro Asn Met Leu Gln Thr Val Gln Asp Ile Val Asp
85 90 95
Tyr Asn Thr Ile Phe Gln Val Ser Tyr Ser Thr Met Ala Asp Ala Ile
100 105 110
Val Gln Lys Asn Asn Lys Lys Leu Lys Val Glu Leu Glu Asn Leu Tyr
115 120 125
Thr Met Ile Leu Glu Asn Gln Lys Lys Ser Asp Glu Leu Leu Thr Lys
130 135 140
Leu Ile Thr Phe Arg Asp Lys Met Thr Val Asp Thr Gln Asn Phe Lys
145 150 155 160
Ile Asp Val Ala Asp Val Ser Ala Ile Leu Ala Glu Lys Glu Thr Gly
165 170 175
Ile Pro Leu Leu Lys Gln Gln Ile Asn Ser Tyr Asn Asp Val Ile Lys
180 185 190
Lys Asn Asn Asp Thr Ile Leu Tyr Gly Glu Ile Leu Cys Ala Thr Gly
195 200 205
Leu Trp Ile Ile Gly Gly Pro Met Ile Ala Val Ala Leu Asp Asn Ile
210 215 220
Asp Asp Ala Glu Glu Glu Ile Gln Asn Leu Met Ser Gln Ile Ser Ile
225 230 235 240
Ala Gln Glu Glu Ser Ile Cys Leu Thr Asn Ile Lys Asn Lys Val Ile
245 250 255
Tyr Leu Thr Asp Thr Ile Asp Val Ala Ile Asp Ser Leu Gln Asn Ile
260 265 270
Ser Asn Arg Trp His Thr Ile Gly Ala Lys Tyr Asn Asn Leu Leu Gln
275 280 285
Asn Leu Asn Leu Leu Asp Ser Asn Glu Leu Thr Phe Ile Lys Glu Asp
290 295 300
Leu Asn Thr Ala Lys Asp Ser Trp Gln Asp Leu Ser Asp Tyr Ala His
305 310 315 320
Glu Leu Asn Lys Gly Ile His Glu Ile Glu Lys Asn
325 330
<210> 171
<211> 343
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 171
Met Ala Glu Thr Glu Thr Gln Leu Asn Arg Ser Thr Glu Asn Ser Tyr
1 5 10 15
Ser Leu Gln Lys Ala Asp Ala Glu Ser Tyr Ile Asp Ile Val Gln Asp
20 25 30
Arg Met Lys Gln Arg Asp Arg Glu Ser Lys Cys Thr Gly Lys Pro Ile
35 40 45
Asn Met Gln Glu Gln Ile Ile Asp Gly Trp Phe Leu Ser Arg Ile Trp
50 55 60
Ile Phe Lys Asp Gln Asn Asn Ser His Gln Thr Asn Arg Phe Ile Ser
65 70 75 80
Trp Phe Lys Asp Asn Leu Ala Ser Ser Lys Gly Tyr Glu Ser Ile Ala
85 90 95
Thr Gln Met Gly Leu Asn Ile Glu Ser Leu Asn Asp Met Glu Thr Ser
100 105 110
Asn Ile Asn Tyr Thr Ser Lys Thr Gly Asp Thr Ile Tyr Asn Gly Val
115 120 125
Ser Glu Leu Thr Asn Asn Thr Gly Thr Thr Gln Lys Met Lys Thr Asp
130 135 140
Ser Phe Gln Arg Asp Tyr Thr Lys Ser Gln Thr Thr Ser Val Thr Asn
145 150 155 160
Gly Leu Gln Val Gly Phe Lys Val Thr Ala Lys Gly Val Leu Phe Leu
165 170 175
Ala Gly Ala Asp Phe Glu Thr Ser Val Asn Tyr Asn Leu Ser Thr Thr
180 185 190
Thr Ala Gln Thr Asn Thr Ile Ser Asp Lys Phe Thr Val Pro Ser Gln
195 200 205
Glu Val Thr Leu Ala Pro Gly His Lys Ala Ile Val Lys His Asp Leu
210 215 220
Arg Lys Met Val Tyr Ser Gly Thr His Asp Leu Lys Gly Asp Leu Lys
225 230 235 240
Val Ser Phe Asn Asp Lys Glu Ile Val Gln Lys Phe Ile Tyr Pro Asn
245 250 255
Tyr Arg Glu Ile Asn Leu Ser Asp Ile Arg Lys Thr Met Ile Ala Ile
260 265 270
Asp Glu Trp Asn His Val Lys Pro Ile Asp Phe Tyr Gln Leu Val Gly
275 280 285
Ile Lys Asn Gln Ile Lys Asn Gly Asp Thr Ile Tyr Ile Asp Thr Pro
290 295 300
Ala Asn Phe Thr Phe Asn Gly Ala Asn Pro Tyr Tyr Arg Ala Thr Phe
305 310 315 320
Thr Glu Tyr Asp Ala Asp Gly Lys Pro Ile Lys Thr Lys Lys Leu Ser
325 330 335
Glu Asn Tyr Lys Leu Lys Asn
340
<210> 172
<211> 287
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 172
Met Ala Gln Ile Gly Ser Val Gln Asn Ile Ala Gln Gln Gln Asn Ala
1 5 10 15
Tyr Ile Ala Asp Trp Arg Glu Pro Phe Glu Ala Ala Tyr Lys Tyr Met
20 25 30
His Asn Leu His Asp Asp Tyr Phe Ile Ser Glu Thr Ala Glu Tyr Lys
35 40 45
Leu Thr Asn Val Tyr Gln Thr Ser Val Glu Gln Ile Gly Ser Pro Thr
50 55 60
Ile Thr Asn Ser Ser Ser Leu Phe Val Gly Arg Ser Thr Leu Thr Asn
65 70 75 80
Asn Thr Asn Gln Asp Gln Thr Leu Thr Thr Asn Glu Phe Ser Lys Thr
85 90 95
Phe Glu Asn Ser Val Thr Asn Ala Thr Thr Asn Gly Phe Asn Leu Gly
100 105 110
Ala Ser Ala Ser Ala Thr Phe Lys Ile Pro Leu Val Gly Glu Thr Ser
115 120 125
Ile Glu Leu Ser Thr Glu Tyr Asn Phe Ser Asp Thr Lys Glu Thr Thr
130 135 140
Lys Ser Glu Ser Tyr Thr Tyr Thr Ala Ser Ala Gln Asn Ile Val Val
145 150 155 160
Pro Ala Asn Ser Ser Val Glu Val Val Val Ser Leu Asn Thr Ala Lys
165 170 175
Ile Asn Gly Asn Val Asn Leu Leu Ser Arg Met Asp Gly Thr Ile Thr
180 185 190
Tyr Asp Arg Ser Leu Asn Gly Gly Tyr Asn Ser Ser Lys Phe Thr Glu
195 200 205
Ser Leu Gly Ser Phe Thr Lys Thr Ala Leu Leu Trp Gly Ser Gln Pro
210 215 220
Gly Leu Arg Val Asp Asn Asn Asn Asn Leu Tyr Leu Val Gly Lys Gly
225 230 235 240
Thr Tyr Ser Ala Glu Tyr Gly Thr Glu Phe Leu Val Ser Val Arg Pro
245 250 255
Val Glu Lys Pro Lys Gly Leu Ala Ala Thr Pro Ser Lys Leu Asn Asn
260 265 270
Glu Gly Tyr Thr Tyr Thr Val Lys Pro Glu Ile Lys Lys Glu Gln
275 280 285
<210> 173
<211> 315
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 173
Met Ala Ala Asn Glu Tyr Gln Phe Leu Thr Glu Asn Asp Leu Arg Asn
1 5 10 15
Gly Val Lys Gln Ala Ile Pro Ser Phe Leu Asp Lys Asn Lys Pro Tyr
20 25 30
Gly Met Tyr Val Glu Tyr Asn Gly Ser Lys Trp Lys Arg Val Ile Asp
35 40 45
Pro Lys Glu Leu Asp Gln Trp Tyr Gln Tyr Pro Gln Leu Val Asn Ala
50 55 60
Val Lys Asp Ile Phe Arg Ala Ser Tyr Thr Asn Tyr Val Gly Leu Lys
65 70 75 80
Asp Leu Thr Ile Glu Asn Gln Asp Ser Lys Ile Thr Ser Leu Arg Lys
85 90 95
Val Gly Asp Pro Glu Leu Ile Gln Leu Gly Thr Ser Glu Leu Val Asn
100 105 110
Thr Asp Ser Glu Thr Lys Gln Asn Leu Thr Ser Ala Pro Phe Ser Lys
115 120 125
Ala Phe Thr Ser Thr Ser Thr Val Thr Val Thr Arg Gly Val Asn Leu
130 135 140
Gly Phe Lys Val Gly Ser Lys Ile Ala Ile Pro Leu Val Gly Ala Asp
145 150 155 160
Ile Ser Thr Glu Phe Ser Arg Asn Thr Asn Trp Ser Thr Asn His Thr
165 170 175
Thr Thr Ser Thr Ser Thr Asp Thr Tyr Thr Ala Pro Ala Gln Thr Ile
180 185 190
Ser Val Pro Pro Arg Thr Lys Ala Ile Val Thr Tyr Val Leu Ser Lys
195 200 205
Gln Gln Tyr Glu Gly Glu Ile Ala Leu Lys Gly Asp Leu Thr Gly Ser
210 215 220
Met Phe Leu Tyr Asp Pro Tyr Ile Val Ala Tyr Pro Gly Met Pro Glu
225 230 235 240
Gln Gln Arg Leu Leu Tyr Thr Ser Thr Leu Tyr Asn Leu Phe Asp Lys
245 250 255
Ser Gly Val Gln Leu Pro Ala Gly Leu Ser Leu Asp Arg Gly Asn Gln
260 265 270
Lys Ile Lys Phe Asn Gly Thr Ala Lys Leu Lys Val Tyr Gly Gly Gln
275 280 285
Met His Asp Val Val Leu Lys Ile Val Ser Leu Asp Asp Pro Asn Lys
290 295 300
Val Ile Glu Glu Arg Lys Ile Pro Lys Lys Asn
305 310 315
<210> 174
<211> 287
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 174
Met Asp Thr Ile Lys Asn Asp Ser Asn Phe Leu Asn Thr Lys Leu Gly
1 5 10 15
Gln Val Gly Gln Glu Gly Ala Leu Gln Asp Ile Asp Asn Ile Leu Asn
20 25 30
Gln Leu Val Asp Ser Ile Asn Lys Asn Ser Pro Leu Met Thr His Ile
35 40 45
Ala Leu Ala Asp Asn Gly Ile Val Asn Tyr Ser Gly Ile Asn Ile Asn
50 55 60
Gln Ser Asn Val Thr Asn Val Ile Pro Leu Phe Leu Gly Asn Asn Thr
65 70 75 80
Phe Ser Asn Thr Ser Ser Glu Glu Gln Ser Tyr Asn Thr Ser Ile Phe
85 90 95
Ser Gln Ala Ile Thr Tyr Thr Lys Ser Thr Ser Ile Gln Asn Gly Phe
100 105 110
Lys Thr Gly Leu Lys Val Gly Gly Lys Ala Gly Ile Pro Phe Val Ala
115 120 125
Glu Gly Ala Val Glu Thr Ser Leu Glu Tyr Asn Phe Ser Ser Thr Gly
130 135 140
Thr Ser Thr Ser Ser Glu Thr Asn Thr Ile Thr Ala Pro Ser Gln Pro
145 150 155 160
Val Lys Val Pro Ala Gly Lys Thr Tyr Lys Val Glu Val Tyr Phe Glu
165 170 175
Lys Lys Ser Thr Ser Gly Thr Val Asp Leu Phe Ala Asp Val Asn Gln
180 185 190
Gly Val Arg Gly Asn Arg Leu His Pro Asp Phe Ala Lys Leu Ser His
195 200 205
Phe Leu Tyr Arg Ala Glu Asp Lys His Gly Val Ile Gln Thr Pro Asn
210 215 220
Asp Pro Asp Ser Ile Arg Ile Lys Gly Ser Gly Asn Phe Arg Ile Glu
225 230 235 240
Tyr Gly Thr Asn Leu Ile Val Lys Thr Tyr Asp Ile Ser Ser Lys Pro
245 250 255
Asn Leu Lys Phe Arg Ser Leu Ile Asn Ser Glu Leu Glu Ser Asp Ile
260 265 270
Leu Val Asn Glu Gln Ile Ile Pro Leu Lys Ser Pro Asn Lys Lys
275 280 285
<210> 175
<211> 234
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 175
Met Thr Ser Ile Tyr Leu Lys Lys Asn Glu Cys Ile Arg Asn Leu Arg
1 5 10 15
Asn Pro Pro Ile Arg Arg Ile Thr Leu Lys Val Pro Cys Lys Asn Lys
20 25 30
Gly Asn Leu Lys Asn Ile Phe Gln Val Glu Pro Gln Tyr Ile Asn Gln
35 40 45
Ala Leu Thr Ala Ser Asn Ala Phe Gln Gly Ala Ile Asp Ser Asn Leu
50 55 60
His Phe Asn Tyr Glu Arg Ala Tyr Gln Ile Gly Val Asn Met Pro Gly
65 70 75 80
Arg Asp Tyr Asp Pro Ser Met Trp Gly Ile Asn Gln Pro Val Ile Gln
85 90 95
Glu Thr Leu Gln Val Ser Ser Met Ile Asn Gln Leu Lys Ser Ile Leu
100 105 110
Asn Arg Phe Leu Tyr Phe Ile Asp Ile Ser Asp Glu Pro Phe Trp Asn
115 120 125
Glu Val Glu Ala Val Ile Val Asn Thr Phe Thr Asn Leu Tyr Thr Gln
130 135 140
Gln Asn Ser Ala Trp Ile Phe Tyr His Ser Ser Thr Ser Thr Ser Thr
145 150 155 160
Ser Tyr Tyr Tyr Asn Ile Met Phe Ala Leu Gln Asp Ser Asn Tyr Arg
165 170 175
Thr Met Ile Val Ile Pro Ile Ala Phe Asp Ile Thr Val Arg Leu Glu
180 185 190
Lys Gln Lys Val Leu Phe Ile Thr Leu Lys Asp Arg Ala Glu Tyr Arg
195 200 205
Val Thr Leu Lys Ser Ile Trp Val Arg Leu Ile Leu Gln Lys Asn Gln
210 215 220
Asp Lys Gln Ile Val Asp Cys Phe Asn Ser
225 230
<210> 176
<211> 354
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 176
Met Ala Thr Asn Asn Ile Thr Ile Ser Gln Ala Ser Ser Asp Thr Phe
1 5 10 15
Lys Glu Val Asn Ile Ser Thr Leu Arg Gly Asp Leu Tyr Glu Arg Phe
20 25 30
Val Thr Leu Phe Glu Gly Asn Pro Arg Leu Leu Gly Ile Asn Ser Asp
35 40 45
Gly Lys Lys Leu Thr Asp Lys Asp Lys Gln Gln Ile Arg Ser Phe Ala
50 55 60
Gln Ala Ile Arg Asp Asp Asn Phe Lys Asn Ile Asp Asp Ile Asp Lys
65 70 75 80
Leu Thr Lys Gly Gln Phe Lys Lys Gly Thr Phe Ile Ala Pro Phe Met
85 90 95
Asn Trp Ser Pro Gln Tyr Ser Asn Leu Thr Thr Asn Tyr Asp Leu Lys
100 105 110
Ala Thr Pro Gly Asp Val Lys His Glu Ser Val Glu Val Tyr Lys Tyr
115 120 125
Lys Asn Pro Lys Asn Ser Val Glu Gln Thr Phe Lys Thr Asp Glu Lys
130 135 140
Glu Val Glu Ile Gly His Thr Phe Thr Glu Thr His Ala Thr Gly Gly
145 150 155 160
Lys Phe Gly Tyr Ser Asn Thr Thr Thr Phe Lys Val Gly Val Pro Leu
165 170 175
Asn Gln Ala Thr Thr Thr His Thr Phe Thr Phe Glu Ala Asn Tyr Asn
180 185 190
Tyr Thr Asn Thr His Thr Lys Thr Asp Lys Glu Thr Lys Arg Tyr Pro
195 200 205
Ser Gln Thr Val Lys Cys Glu Pro Gly Tyr Ile Thr Gln Leu Thr Ile
210 215 220
Asp Lys Ser Thr Ala Thr Tyr Phe Gly Glu Leu Glu Phe Asn Ile Gly
225 230 235 240
Ser Ser Ala Lys Glu Leu Met Asn Gln Ile Asn Gln Asn Ile Lys Asp
245 250 255
Ile Asn Ala Phe Ser Lys Asn Lys Leu Ser Val Ile Gln Pro Asp Glu
260 265 270
Glu Glu Leu Met Tyr Thr Leu Tyr Lys Tyr Ser Gly Tyr Asp Ile Pro
275 280 285
Ser Tyr Val Thr Leu Asp Asp Glu His Lys Thr Val Ser Phe Gly Lys
290 295 300
Ile Lys Ser Thr Tyr Thr Gly Val Thr Gly Thr Ala Ser Asp Met Ile
305 310 315 320
Leu Glu Leu Thr Pro Ile Asp Ser Lys Asp Thr Thr Lys Lys Ser Ile
325 330 335
Lys Met Pro Phe Lys Glu Tyr Gln Gln Lys Arg Lys Asn Asn Glu Pro
340 345 350
Ile Ile
<210> 177
<211> 192
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 177
Met Ala Ala Ser Phe Arg Thr Val Val Glu Val Ala Pro Asp His Leu
1 5 10 15
Asp Gln Ala Arg Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro
20 25 30
Ala Thr Val Asn Phe Asp Phe Glu Ala Ile His Arg Ala Ala Ser Ala
35 40 45
Val Pro Asp Ser Asp Val Val Lys Met Val Arg Gly Trp Gly Leu Gln
50 55 60
Glu Tyr Ala Pro Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val
65 70 75 80
Arg Gln Ala Leu Pro Pro Glu Cys Ala Glu Ala Gly Phe Trp Ala Thr
85 90 95
Val Glu Arg Glu Leu Val Gln Ala Phe Thr Gly Leu Ala Ala Gln Glu
100 105 110
Gly Gln Pro Gly Leu Ser Phe Tyr Glu Glu Ser Gly Asp Arg Thr Arg
115 120 125
Phe Tyr Arg Asp Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Asp
130 135 140
His Met Tyr Ala Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro
145 150 155 160
Lys Ala Glu Thr Leu Ala Leu Asp Leu Thr Asp Ile Val Pro Phe Ala
165 170 175
Val Arg Leu Asn Ala Ile Val Val Arg Gln Ala Leu Ser Val Ala Val
180 185 190
<210> 178
<211> 789
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 178
Met Gln Glu Lys Gln Glu Gln Gly Leu Lys Glu Thr Gln Val Thr Pro
1 5 10 15
Phe Asn Glu Leu Lys Glu Lys Ile Thr Glu Asn Pro Asn Leu Asp Asn
20 25 30
Ser Arg Met Trp Asn Pro Tyr Lys Gly Val Asp Tyr Val Lys Phe Met
35 40 45
Asn Ala Leu Asn Ser Asn Val Trp Lys Pro Val Leu Thr Asp Ala Val
50 55 60
Lys Asn Glu Gly Glu Asn Val Val Pro Val Leu Ile Lys Gly Ala Ile
65 70 75 80
Val Met Gly Leu Ser Leu Ala Pro Pro Pro Leu Pro Met Leu Gly Thr
85 90 95
Val Val Asp Leu Phe Gln Phe Thr Asp Gly Lys Thr Asp Asp Ala Trp
100 105 110
Ser Arg Thr Val Glu Tyr Val Asn Lys Thr Ile Asp Ser Lys Ile Glu
115 120 125
Gln Tyr His Ser Tyr Leu Met Ser Ser Glu Leu Val Ala Leu Gly Ser
130 135 140
Ser Leu Gly Glu Tyr Lys Arg Val Leu Glu Ile Tyr Glu Thr Asn Lys
145 150 155 160
Leu Thr Arg Val Glu Asp Pro Ala Ala Gly Val Arg Glu Gln Phe Arg
165 170 175
Ile Ser Asn Asp Lys Ala Lys Ala Phe Ile Ala Lys Leu Gln His Pro
180 185 190
Gln Asn Gln Gly Asp Thr Arg Tyr Asn Val Leu Thr Leu Pro Leu Phe
195 200 205
Ala Gln Ala Ala Asn Leu His Leu Ala Leu Leu Arg Asp Ala Ile Val
210 215 220
Cys Asp Trp Gly Ile Asp Ser Asn Gln Leu Gln Gly Tyr Lys Asp Glu
225 230 235 240
Leu Lys Lys Ser Ile Ile Asp Tyr Thr Asn Tyr Ile Asn Lys Thr Tyr
245 250 255
Lys Glu Gly Leu Glu Gln Glu Lys Asn Lys Ala Ala Asp Tyr Ser Asp
260 265 270
Pro Glu Ser Tyr Ile Gln Ala Gly Lys Ser Asp Val Pro Val Leu Arg
275 280 285
Gln Trp Phe Pro Glu Val Met Lys Trp Asn Arg Val Ala Asn Tyr Glu
290 295 300
Arg Gln Met Ser Ala Ser Val Leu Asp Leu Ala Ala Val Leu Pro Thr
305 310 315 320
Tyr Asp Pro Asp Asn Tyr Ser Lys Gln Pro Ile Val Lys Gln Ser Arg
325 330 335
Pro Ile Phe Ala Pro Ile Ile Gly Ile Pro Gly Gly Leu Lys Ser Lys
340 345 350
Asp Ser Ala Gly Thr Phe Gly Ser Tyr Val Phe Asp Val Lys Asn Phe
355 360 365
Asp Gln Ile Asn Thr Glu Leu Ala Lys Ser Pro Tyr Asp Tyr Leu Ala
370 375 380
Tyr Gly Gly Ala Leu Asn Gln Ile Gly Ile Arg Thr Ser Gly Tyr Ile
385 390 395 400
Tyr Gly Ser Ser Thr Asn Thr Thr Tyr Tyr Thr Asn Met Thr His Gly
405 410 415
Ser Thr Ala Gly Asn Gln Thr Asn Ile Ser Ile Asn Pro Asn Asn Pro
420 425 430
Ile Thr Ser Ile Ser Gly Thr Ala Gly Thr Lys Pro Tyr Thr Leu Ser
435 440 445
Phe Gln Phe Ala Asp Gly Gly Thr Thr Gln Thr Leu Gly Ser Asn Val
450 455 460
Pro Asn Val Asn Cys Tyr Ala Pro Phe Lys Phe Glu Tyr Pro Gly Tyr
465 470 475 480
Gln Leu Ser Ala Val Arg Val Phe Gly Thr Ser Ala Ser Thr Gly Asp
485 490 495
Ala Asp Ala Met Val Phe Ala Phe Arg Ser Thr Glu Ile Gly Asp Thr
500 505 510
His Gln Leu Met Ala Asn Val Pro Thr Gln Ile Pro Ala Glu Ile Gly
515 520 525
Tyr Gly Gln Glu Asn Thr Leu Asn Glu Val Ile Asn Gly Gln Asn Ala
530 535 540
Ile Glu Met Asn Asn Gly Asn Thr Met Lys Tyr Ser Ile Asn Ser Pro
545 550 555 560
Lys Thr Gln Gln Tyr Lys Val Arg Tyr Arg Val Ala Ala Asn Glu Glu
565 570 575
Ser Ser Val Arg Leu Gln Asn Met Asp Thr Asn Ile Ser Lys Thr Thr
580 585 590
Asp Thr Ser Asn Thr Ser Arg Asn Thr Val Lys Gly Glu Tyr Gly Tyr
595 600 605
Tyr Lys Leu Val Glu Gly Pro Thr Ile Thr Leu Asn Lys Gly Ile Asn
610 615 620
Thr Val Thr Leu Gln Gly Thr Lys Gly Lys Phe Ala Leu Asp Tyr Ile
625 630 635 640
Glu Val Glu Pro Ile Val Gln Asp Glu Arg Val Tyr Phe Glu Asp Phe
645 650 655
Ile Gly Asp Leu Lys Ser Ser Trp Tyr Gly Ser Asn Ala Gly Phe Ala
660 665 670
Pro Gly Tyr Gly Thr Val His Glu Asn Gly Glu Leu Tyr Thr Phe Ile
675 680 685
Asn Glu Lys Val Glu Lys Tyr Ser Gln Tyr Thr Met Arg Val Lys Leu
690 695 700
Lys Leu Asn Ser Asn Asp Pro Asn Val Arg Glu Asn Val Arg Leu Phe
705 710 715 720
Thr Asp Asn Ala Lys Gly Glu Arg Ile Thr Lys Glu Val Thr Leu Lys
725 730 735
Gly Gly Ala Asn Tyr Gln Glu Phe Lys Leu Glu Phe Met Thr Asn Asp
740 745 750
Asn Leu Asp Lys Thr His Ile Gly Val Gln Lys Ala Ser Gly Ser Asn
755 760 765
Thr Val Phe Val Lys Asp Val Glu Val Ile Gly Thr Pro Asn Asp Asn
770 775 780
Ala Thr Pro Tyr Lys
785
<210> 179
<211> 645
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 179
Met Gln Glu Lys Gln Glu Gln Gly Leu Lys Glu Thr Gln Val Thr Pro
1 5 10 15
Phe Asn Glu Leu Lys Glu Lys Ile Thr Glu Asn Pro Asn Leu Asp Asn
20 25 30
Ser Arg Met Trp Asn Pro Tyr Lys Gly Val Asp Tyr Val Lys Phe Met
35 40 45
Asn Ala Leu Asn Ser Asn Val Trp Lys Pro Val Leu Thr Asp Ala Val
50 55 60
Lys Asn Glu Gly Glu Asn Val Val Pro Val Leu Ile Lys Gly Ala Ile
65 70 75 80
Val Met Gly Leu Ser Leu Ala Pro Pro Pro Leu Pro Met Leu Gly Thr
85 90 95
Val Val Asp Leu Phe Gln Phe Thr Asp Gly Lys Thr Asp Asp Ala Trp
100 105 110
Ser Arg Thr Val Glu Tyr Val Asn Lys Thr Ile Asp Ser Lys Ile Glu
115 120 125
Gln Tyr His Ser Tyr Leu Met Ser Ser Glu Leu Val Ala Leu Gly Ser
130 135 140
Ser Leu Gly Glu Tyr Lys Arg Val Leu Glu Ile Tyr Glu Thr Asn Lys
145 150 155 160
Leu Thr Arg Val Glu Asp Pro Ala Ala Gly Val Arg Glu Gln Phe Arg
165 170 175
Ile Ser Asn Asp Lys Ala Lys Ala Phe Ile Ala Lys Leu Gln His Pro
180 185 190
Gln Asn Gln Gly Asp Thr Arg Tyr Asn Val Leu Thr Leu Pro Leu Phe
195 200 205
Ala Gln Ala Ala Asn Leu His Leu Ala Leu Leu Arg Asp Ala Ile Val
210 215 220
Cys Asp Trp Gly Ile Asp Ser Asn Gln Leu Gln Gly Tyr Lys Asp Glu
225 230 235 240
Leu Lys Lys Ser Ile Ile Asp Tyr Thr Asn Tyr Ile Asn Lys Thr Tyr
245 250 255
Lys Glu Gly Leu Glu Gln Glu Lys Asn Lys Ala Ala Asp Tyr Ser Asp
260 265 270
Pro Glu Ser Tyr Ile Gln Ala Gly Lys Ser Asp Val Pro Val Leu Arg
275 280 285
Gln Trp Phe Pro Glu Val Met Lys Trp Asn Arg Val Ala Asn Tyr Glu
290 295 300
Arg Gln Met Ser Ala Ser Val Leu Asp Leu Ala Ala Val Leu Pro Thr
305 310 315 320
Tyr Asp Pro Asp Asn Tyr Ser Lys Gln Pro Ile Val Lys Gln Ser Arg
325 330 335
Pro Ile Phe Ala Pro Ile Ile Gly Ile Pro Gly Gly Leu Lys Ser Lys
340 345 350
Asp Ser Ala Gly Thr Phe Gly Ser Tyr Val Phe Asp Val Lys Asn Phe
355 360 365
Asp Gln Ile Asn Thr Glu Leu Ala Lys Ser Pro Tyr Asp Tyr Leu Ala
370 375 380
Tyr Gly Gly Ala Leu Asn Gln Ile Gly Ile Arg Thr Ser Gly Tyr Ile
385 390 395 400
Tyr Gly Ser Ser Thr Asn Thr Thr Tyr Tyr Thr Asn Met Thr His Gly
405 410 415
Ser Thr Ala Gly Asn Gln Thr Asn Ile Ser Ile Asn Pro Asn Asn Pro
420 425 430
Ile Thr Ser Ile Ser Gly Thr Ala Gly Thr Lys Pro Tyr Thr Leu Ser
435 440 445
Phe Gln Phe Ala Asp Gly Gly Thr Thr Gln Thr Leu Gly Ser Asn Val
450 455 460
Pro Asn Val Asn Cys Tyr Ala Pro Phe Lys Phe Glu Tyr Pro Gly Tyr
465 470 475 480
Gln Leu Ser Ala Val Arg Val Phe Gly Thr Ser Ala Ser Thr Gly Asp
485 490 495
Ala Asp Ala Met Val Phe Ala Phe Arg Ser Thr Glu Ile Gly Asp Thr
500 505 510
His Gln Leu Met Ala Asn Val Pro Thr Gln Ile Pro Ala Glu Ile Gly
515 520 525
Tyr Gly Gln Glu Asn Thr Leu Asn Glu Val Ile Asn Gly Gln Asn Ala
530 535 540
Ile Glu Met Asn Asn Gly Asn Thr Met Lys Tyr Ser Ile Asn Ser Pro
545 550 555 560
Lys Thr Gln Gln Tyr Lys Val Arg Tyr Arg Val Ala Ala Asn Glu Glu
565 570 575
Ser Ser Val Arg Leu Gln Asn Met Asp Thr Asn Ile Ser Lys Thr Thr
580 585 590
Asp Thr Ser Asn Thr Ser Arg Asn Thr Val Lys Gly Glu Tyr Gly Tyr
595 600 605
Tyr Lys Leu Val Glu Gly Pro Thr Ile Thr Leu Asn Lys Gly Ile Asn
610 615 620
Thr Val Thr Leu Gln Gly Thr Lys Gly Lys Phe Ala Leu Asp Tyr Ile
625 630 635 640
Glu Val Glu Pro Ile
645
<210> 180
<211> 308
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 180
Met Leu Lys Asn Ile Ser Asp Val Asn Gln Asp Arg Ile Val Asp Val
1 5 10 15
Ser Glu Thr Met Glu Phe Ile Ala Gln Asn Tyr Tyr Glu Ile His Met
20 25 30
Lys Asp Lys Leu Leu Leu Cys Trp Val Ser Gly Ser Asp Gly Tyr Met
35 40 45
Ile Arg Lys Leu Asp Lys Ile Thr Pro Gly Glu Thr Trp Ser Glu Ile
50 55 60
Glu Gln Ser Ser Ile Gln Thr Ser Asn Leu Gln Leu Ser Pro Glu His
65 70 75 80
Leu Glu Asn Val Gln Val Ser Leu Leu Asp Asn Thr Leu Asn Ile Asn
85 90 95
Gln Ala Met Ser Ser Thr Ser Tyr Cys Lys Ala Val Thr Asn Thr Ser
100 105 110
Thr Thr Gln Thr Asn Gln Gly Val Lys Ala Ser Ala Glu Ala Lys Leu
115 120 125
Leu Pro Ile Pro Leu Ile Met Pro Asp Gly Ile Lys Leu Thr Ala Asp
130 135 140
Val Asn Ser Ser Thr Asp Thr Asn Ile Ser Lys Thr Thr Thr Glu Thr
145 150 155 160
Ile Ile Val Pro Pro Gln Thr Val Ile Val Lys Pro His His Lys Val
165 170 175
Lys Val Thr Ala Glu Phe Tyr Thr Asn Lys Tyr Ser Gly Asp Ile Ile
180 185 190
Tyr Asn Thr Lys Val Asn Lys Ser Ser Thr Gly Leu Lys Val Asn Ala
195 200 205
Glu Tyr Tyr Val Pro Phe Asn Pro Phe Ile Gly Trp Leu Thr Pro Asn
210 215 220
Arg His Asp Thr Ile Thr Leu Asp Asn Tyr Glu Met Tyr Arg Gly Asp
225 230 235 240
Asp Ser Ser Ile Glu Lys Arg Leu Ile Gln Ala Gly Val Lys Phe Asn
245 250 255
Ala Asp Gly Ser Met Gln Ala Leu Cys Lys Thr His Phe Asp Ser Thr
260 265 270
Ser Gly Ser Tyr Ala Lys Ile Ser Val Ile Asp Val Thr Asn Leu Ser
275 280 285
Cys Pro Leu Ile Leu Lys Glu Gln Val Tyr Lys Asn Phe Gln Thr Asn
290 295 300
Asn Ser Asn Ser
305
<210> 181
<211> 477
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 181
Met Ala Ala Thr Asn Ala Gln Lys Gly Thr Asp Ile Ile Gln Ile Met
1 5 10 15
Ser Asp Gly Ser Ile Lys Ile Val Asn Asp Gln Leu Asp Lys Gln Ser
20 25 30
Pro Ala Lys Ile Leu Thr Asn Leu Gly Gly Ser Ala Phe Ile Asn Leu
35 40 45
Val Lys Gly Ala Gln Asn Asn Gly Met Thr Thr Val Asn Asn Thr Ala
50 55 60
Arg Asp Ile Val Met Ala Gly Thr Ser Gly Ile Pro Tyr Gly Gly Ala
65 70 75 80
Ile Ile Ser Ser Leu Ile Gly Leu Leu Trp Pro Gln Asp Gly Val Asn
85 90 95
Gln Met Lys Gln Met Met Glu Glu Met Ala Lys Met Met Asp Gln Lys
100 105 110
Ile Glu Asp Phe Asp Lys Ala Gly Leu Asn Gln Gln Ile Lys Asp Ala
115 120 125
Gln Gly Ala Leu Arg Glu Met Glu Leu Val Leu Tyr Gly Asp Gly Ser
130 135 140
Gly Ser Thr Arg Ser Leu Pro Gly Thr Ala Ser Glu Lys Ala Gly Leu
145 150 155 160
Ala His Ser Lys Leu Met Thr Ala Leu Ser Ala Ala Gln Lys Glu Gly
165 170 175
Tyr Asp Arg Thr Glu Leu Pro Ala Tyr Thr Ala Ala Ala Thr Ala His
180 185 190
Leu Leu Phe Leu Ser Phe Met Val Glu Asn Gly Glu Ser Pro Leu Phe
195 200 205
Lys Ile Asp Ser Asp Ser Met Asn Arg Thr Tyr Lys Ile Tyr Leu Lys
210 215 220
Glu Ala Ile Ser Asp Tyr Lys Thr His Ile Asn Arg Thr Val Gly Asp
225 230 235 240
Gly Arg Asn Ser Leu Tyr Gln Ala Asn Tyr Arg Ile Gln Glu Ile Asn
245 250 255
Lys Glu Ile Ala Arg Leu Ala Lys Arg Gln Glu Thr Ser Gly Pro Lys
260 265 270
Gly Phe Leu Gln Leu Gln Asp Lys Val Asn Glu Gln Leu Gly Tyr Lys
275 280 285
Asn Asp Val Leu Lys Asp Lys Ala Lys Phe Asp Asn Tyr Val Asn Ser
290 295 300
Thr Val Asn Asn Ala Ala Phe Asn Glu Ala Leu Asn Phe Ala Ile Asp
305 310 315 320
Thr Ala Thr Lys Asn Lys Ile Ser Ile Thr Asp Pro Arg Ser Gln Ile
325 330 335
Ala Asn Gly Val Tyr Val Ile Ala Ser Gly Leu Asp Asn His Met Val
340 345 350
Leu Asp Asn Asp Asn Gly Thr His His Asn Val Ala Thr Tyr Glu Glu
355 360 365
His Asp Gly Lys Asn Gln Arg Trp Leu Ile Ser Tyr Glu Lys Asn Lys
370 375 380
Gln Ala Tyr Lys Ile Gln Asn Leu Gly Asp Asn Gly Pro Val Leu Ala
385 390 395 400
Thr Gly Ser Ser Ala Asn Tyr Asn Val Phe Gly Tyr Glu Phe Ser Gly
405 410 415
Ser Asp Asp Gln Tyr Trp Ile Pro Glu Ser Val Gly Asp Gly Tyr Tyr
420 425 430
Ile Leu Lys Asn Lys Ala Phe Pro Asp Arg Val Leu Asp Val Ser Asn
435 440 445
Gly Lys Ile Gly Asn Gly Thr Asn Val Thr Ile Tyr Pro Arg Asp Ala
450 455 460
Lys Asn Pro Ala Asn Gln Lys Phe Lys Phe Ile Ala Gln
465 470 475
<210> 182
<211> 312
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 182
Met Ala Pro Val Ala Ser Val Val Asn Asp Gln Phe Gly Gln Gly Ser
1 5 10 15
Thr Tyr Ser Thr Glu Asn Ile Ser Asn Val Leu Phe Val Arg Leu Arg
20 25 30
Ala Gly Ile Glu Ala Ser Pro Glu Leu Arg Gln Lys Phe Lys Ile Ala
35 40 45
Asp Asp Glu Lys Ile Gln Phe Glu Thr Gly Glu Gly Gly Gly Thr Ile
50 55 60
Gly Gly Asp Lys Val Asn Ala Ile Asn Tyr Gly Leu Arg Asp Gln Leu
65 70 75 80
Ser Lys Val Thr Ala Ser Ser Thr Gly Thr Asn Ile Thr Val Asn Pro
85 90 95
Thr Thr Val Thr Glu Val Glu Ala Gly Lys Ile Glu Leu Leu Ser Tyr
100 105 110
Arg Asn Asp Thr Pro Ile Thr Gln Lys Gln Phe Thr Thr Glu Lys Gln
115 120 125
Val Lys Arg Val Asp Thr Val Thr Ala Thr Asn Gln Tyr Gly Phe Lys
130 135 140
Leu Gly Phe Glu Ser Thr Thr Ser Phe Glu Val Asn Ala Leu Val Ala
145 150 155 160
Lys Ala Glu Gln Ser Phe Lys Ala Thr Ala Glu Phe Asn Phe Ser His
165 170 175
Thr Asp Thr Asn Thr Val Thr Asn Glu Glu Thr Val Thr Phe Lys Ala
180 185 190
Gln Glu Val Gln Ala Ala Val Gly Gly Thr Thr Asn Tyr Phe Thr Arg
195 200 205
Val Gly Lys Ala Glu Phe Ser Gly Ser Phe Gln Thr Asp Ala Ala Leu
210 215 220
Thr Asp Leu Lys Leu Lys Leu Pro Ile Val Lys Gln Gly Asn Asn Glu
225 230 235 240
Thr Val Val His Thr Glu Glu Val Thr Leu Thr Ser Gln Asp Ile Tyr
245 250 255
Thr Ile Phe Lys Asn Thr Pro Tyr Ile Pro Leu Pro Ala Tyr Leu Ser
260 265 270
Phe Asp Asp Asn Gly Lys Lys Val Leu Val Lys Asn Val Thr Phe Asn
275 280 285
Tyr Thr Gly Glu Met Gly Tyr Asn Thr Ala Ala Gln Ala Glu Phe Ile
290 295 300
Pro Phe Asp Gln Asp Asn Pro Lys
305 310
<210> 183
<211> 304
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 183
Met Ile Val Asp Val Glu Arg Asp Leu Ile Asp Asn Asn Glu Trp Tyr
1 5 10 15
Ala Arg Val Thr Tyr Asn Ala Asn Pro Ser Thr Phe Arg Asn Gln Ser
20 25 30
Val Tyr Asp Met Asn Val Ser Asp Leu Asp Val Val Pro Ile Thr Thr
35 40 45
Glu Phe Glu Ser Thr Pro Gln Leu Thr Asn Ser Ala Ser Gln Ile Val
50 55 60
Arg Asn Tyr Thr Ser Lys Asp Gln Ser Gln Thr Val Leu Phe Ser Glu
65 70 75 80
Lys Ser Ile Glu Thr Phe Ser Arg Ser Thr Thr Glu Gly Tyr Lys Ile
85 90 95
Gly Ser Ser Ile Lys Ser Thr Thr Ser Phe Lys Val Lys Val Gly Phe
100 105 110
Leu Val Ser Gly Glu Ile Ser Gln Ser Ile Glu Val Ala Val Thr Gly
115 120 125
Glu Tyr Asn His Ser Ser Thr Glu Thr Thr Thr Thr Thr Asn Glu Lys
130 135 140
Leu Trp Gln Val Thr Gln Pro Val Ile Ile Pro Pro Tyr Thr Gln Val
145 150 155 160
Thr Ala Thr Leu Gln Ile Phe Ser Gly Pro Phe Val Val Pro Ala Lys
165 170 175
Thr Lys Ala Thr Ile Gln Gly Lys Gly Thr Asn Asn Gly Ala Tyr Asn
180 185 190
Phe Ala Ser Val Ile Thr Tyr Thr Asp Tyr Gly Gly Lys Thr Trp Thr
195 200 205
Asp Arg Asn Arg Ala Gln Ala Leu Tyr Thr Asp Arg Gly Gln Trp Pro
210 215 220
Gly Tyr Lys Pro Ile Tyr Val Gly Gly Ser Ser Ser Thr Asp Pro Thr
225 230 235 240
Gly Leu Leu Arg Leu Glu Gly Glu Ala Arg Ile Asn Ala Gln Val Gly
245 250 255
Leu Tyr Ala Val Thr Glu Phe Lys Glu Ser Pro Leu Pro Gly Tyr Ala
260 265 270
Asp Ile Gly Ser Asn Arg Thr Tyr Tyr Ala Pro Asn Ile Ile Leu Gly
275 280 285
Asp Gly Arg Ile Ile Pro Val Pro Gly Tyr Gln Arg Tyr Leu Gln Arg
290 295 300
<210> 184
<211> 935
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 184
Met Phe Ser Ser Ala Leu Asp Asn Thr Ser Val Leu Leu Asn Leu Ile
1 5 10 15
Glu Lys Gly Ile Asn Glu Gly Asp Asp Leu Leu Gly Leu Phe Ser Phe
20 25 30
Ile Gly Leu Thr Ala Leu Glu Ala Ile Pro Ile Val Gly Gly Ala Leu
35 40 45
Ser Lys Leu Ile Ser Ile Ile Phe Phe Ser Ser Lys Pro Ser Ile Ser
50 55 60
Tyr Gln Lys Ile Trp Glu Glu Leu Val Lys Ala Ile Asn Gln Ile Val
65 70 75 80
Asp Lys Lys Ile Glu Glu Ala Leu Val Ser Glu Leu Ile Gln Glu Leu
85 90 95
Ser Gly Phe Ala Asn Val Leu Glu Glu Tyr Arg Asp Ala Tyr Asp Leu
100 105 110
Tyr Asn Gly Lys Arg Val Phe Glu Ile Pro Asp Asn Leu Thr Pro Glu
115 120 125
Glu Tyr Leu Val Thr Val Phe Thr Ala Ala Asn Leu Gln Phe Leu Gln
130 135 140
Arg Ile Pro Arg Phe Gln Asn Pro Lys Phe Asp Val Val Phe Leu Pro
145 150 155 160
Phe Phe Val His Val Ala Glu Met His Ile Leu Leu Ile Arg Asp Ala
165 170 175
Ala Ile His Gly Leu Glu Trp Gly Met Asp Glu Lys Met Asn Gln Lys
180 185 190
Phe Lys Lys Asp Leu Lys Asn Cys Ile Glu Glu Tyr Thr Thr Tyr Leu
195 200 205
Leu Lys Ile Tyr Lys Lys Gly Leu Lys Glu Ala Ser Glu Arg Glu Leu
210 215 220
Lys Asp Lys Asp Phe Pro Thr Thr Arg Asp Lys Lys His Tyr Ile Asn
225 230 235 240
Thr Val Arg Trp Asn Val Ile Asn Gln Tyr Lys Arg Gly Met Thr Leu
245 250 255
Thr Val Phe Asp Phe Ala Tyr Arg Trp Lys Tyr Tyr Gln Glu Asp Tyr
260 265 270
Arg Asn Asn Ile Thr Leu Asn Pro Ile Arg Thr Ile Tyr Ser Asp Ile
275 280 285
Ala Gly Ser Val Tyr Pro Tyr Glu Lys Thr Thr Asn Glu Ile Asp Ile
290 295 300
Thr Ile Lys Asp Gln Asn Val Lys Tyr Arg Gly Leu Leu Lys Lys Leu
305 310 315 320
Gln Val Tyr His Ala Glu Arg Ile Asp Ser Ile Gln Ser Thr Tyr Leu
325 330 335
Arg Asn His Glu Ile Ile Asn Ser Asn Lys Ile Gly Gly Thr Gly Gly
340 345 350
Arg Ile Ser Ser Leu Asp Leu Glu Asp His Ile Asn Asn Pro Leu Ile
355 360 365
Gln Val Asn Met Trp Ser Glu Leu Val Pro Ile Ser Leu Gly Phe Lys
370 375 380
Phe Tyr Asn Glu Lys Glu Glu Lys Ile Gly Gly Gly Ser Trp Trp Pro
385 390 395 400
Asn Pro His Lys Phe Gly Ala Tyr His Phe Val Gly Asn Lys Val Ser
405 410 415
Ser Ile Ile Gly Phe Gly Lys Asn Glu Thr Gly Gly Phe Asn Ser Leu
420 425 430
Asp Ala Met Ala Val Gly Phe Lys Leu Asp Asp Tyr Asp Ser Lys Asn
435 440 445
Arg Leu Phe Gly Ile Asn Lys Asn Gly Glu Pro Val Thr Lys Val Ile
450 455 460
Asp Ala Gly Asn Phe Tyr Lys Asp Asn Phe Lys Ser Asn Ile His Lys
465 470 475 480
Ala Asp Glu Pro Ile Phe Gly Asp Gly Ile Leu Gln Phe Thr Asn Tyr
485 490 495
Leu Ser His Gln Asn Gln Asp Ser Ser Val Met Tyr Gln Ile His Val
500 505 510
Glu Ile Glu Gly Thr Tyr Gln Leu His Ala Ile Ile Gly Ala Lys Lys
515 520 525
Gln Arg Glu Lys Leu Ser Ile Lys Val Ile Leu Asn Asp Glu Gln Gln
530 535 540
Lys Asn Leu Ile Thr Asp Tyr Phe Asp Ser Ser Asn Thr Trp Thr Gly
545 550 555 560
Met Ser Leu Asn Asn Glu Trp Val Tyr Lys Arg Val Leu Ile Gly Asn
565 570 575
Phe Gln Leu Lys Arg Gly Ile Asn Asn Ile Asn Ile His Asn Ser Ile
580 585 590
Leu Gln Asp Ser Ala Asn Ile Lys Thr Trp Asn Leu Gly Ser Ile Glu
595 600 605
Leu Thr Met Asp Thr Asn Ser Ile Lys Asp Pro Asp Val Thr Thr Leu
610 615 620
Tyr Asp Lys Asp Asn Tyr Ala Gly Thr Lys Arg Phe Ile Phe Glu Asp
625 630 635 640
Thr Arg Arg Leu Ile Asp Phe Asn Asp Arg Thr Ser Ser Ile Lys Val
645 650 655
Gly Ser Tyr Val Pro Gly Leu Arg Phe Tyr Glu His Tyr Asp Tyr Gly
660 665 670
Gly Gly Phe Ile Asp Leu Gly Ser Gly Glu Lys Leu Ser Leu Lys Asn
675 680 685
His Arg Leu Asn Lys Lys Leu Ser Ser Ala Lys Phe Ala Asn Leu Val
690 695 700
Leu Tyr Lys Glu Asp Asp Tyr Lys Gly Glu Lys Thr Leu Ile Phe His
705 710 715 720
Asp Ile Pro Tyr Leu Thr Asp Phe Asn Asp Lys Thr Ser Ser Leu Val
725 730 735
Val Ser Ser Asn Val Ser Gly Ile Val Arg Leu Phe Glu His Ser Phe
740 745 750
Tyr Asn Gly Glu Tyr Ile Asp Val Ser Gly Gly Gln Lys Ile Ser Leu
755 760 765
Lys Gly His Asn Leu Asn Arg Arg Ile Ser Ser Ile Lys Phe Leu Lys
770 775 780
Glu His Glu Val Phe Asn Gly Leu Tyr Glu Ile Val Thr Ala Ile Asn
785 790 795 800
Asn Lys Ser Val Val Asp Trp Ser Lys Asp Lys Asn Ser Asn Val His
805 810 815
Leu Trp Glu Arg Arg Asn Gln Asp Asn Gln Lys Trp Tyr Phe Lys Tyr
820 825 830
Asp Ser Glu Lys Gln Ala Phe Gln Ile Ile Ser Lys Ala Asp Glu Tyr
835 840 845
Glu Val Leu Ala Trp Asn Asp Phe Lys Asn Ser Met Asn Val Phe Ala
850 855 860
Thr Tyr Asn Ser Lys Lys Asp Glu His Tyr Trp Ile Leu Asp Tyr Leu
865 870 875 880
Gly Glu Gly Asn Tyr Leu Ile Arg Asn Lys Lys Lys Gln Asn Leu Val
885 890 895
Leu Asp Val Asp Gly His Lys Thr Ala Asn Gly Thr Asn Ile Lys Val
900 905 910
Asn Glu Gln His Ala Leu Asp Asn Pro Ser Ile Glu Ala Gln Lys Phe
915 920 925
Lys Ile Asn Leu Val Asp Asn
930 935
<210> 185
<211> 459
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 185
Met Glu Glu Gln Gly Gly Ile Lys Glu Gln Leu Thr Lys Leu Lys Asp
1 5 10 15
Glu Ile Asn Ala Glu Thr Asp Lys Lys Ile Ala Gln Gln His Leu Ile
20 25 30
Asp Leu Asp Ser Lys Phe Ser Asp Leu Leu Glu Asn Leu Asn Lys Leu
35 40 45
Glu Asn Ser Val Lys Ser Lys Lys Ala Glu Met Tyr Tyr Asp Ser Gln
50 55 60
Gly Ser Ile Glu Glu Ser Arg Arg Ile Trp Val Gly Ile Ile Glu Ser
65 70 75 80
Lys Phe Gly Glu Ile Leu Thr Leu Ala Lys Gln Glu Lys Asn Lys Val
85 90 95
Asn Glu Leu Pro Leu Tyr Thr Gln Val Ala Thr Ala His Ile Ser Phe
100 105 110
Leu Lys Asn Leu Glu Ile Asn Gly Pro Gly Pro Asn Tyr Lys Phe Asp
115 120 125
Pro Gln Ser Leu Lys Tyr Phe Tyr Asn Thr Lys His Ile Gln Glu Thr
130 135 140
Ile Asp Lys Tyr Thr Thr Tyr Ile Lys Asn Thr Phe Asp Asp Phe His
145 150 155 160
Ile Lys Asn Ile Glu Lys Ile Tyr Lys Val Tyr Gly Ser Pro Ile Asp
165 170 175
Pro Tyr Ile Phe Ala Thr Ser Glu Gly Arg Glu Leu Val Lys Gln Lys
180 185 190
Leu Asn Lys Leu Leu Leu Glu Ala Lys Ala Thr Leu Asp Lys Ile Asn
195 200 205
Asn Glu Thr Val Trp Leu Gly Gly Trp Ala Ala His Gly Arg Ser Val
210 215 220
Glu Lys Ala Thr Asp Glu Leu Asn Asn Val Lys Val Ala Val Asn Ala
225 230 235 240
Ala Asp Phe Ser Ser Lys Leu Phe Glu Thr Thr Ile Ala Asp Glu Ser
245 250 255
Phe Asn Thr Ile Ala Ser Leu Gly Thr Trp Ile Glu Lys Asp Gly Lys
260 265 270
His Tyr Tyr Asn Asp Arg Tyr Gly Glu Met Val Thr Glu Trp Leu Asn
275 280 285
Ile Gly Lys Lys Thr Tyr Phe Phe Ser Pro Glu Asp Asn Val Glu Asn
290 295 300
Leu Ser Gly Ile Lys Phe Lys Lys Gly Glu Met Ile Thr Gly Trp Val
305 310 315 320
Asn Thr Glu Glu Asn Met Glu Ser Asn Gln Ile Arg Ser Tyr Leu Phe
325 330 335
Val Asp Glu Glu Glu Gly Leu Lys Asn Ser Ala Gly Glu Leu Phe Thr
340 345 350
Leu Gly Gln Met Met Thr Gly Trp His Lys Thr Met Asp Gly Lys Asp
355 360 365
Lys Glu Phe Trp Tyr Tyr Phe Thr Ser Glu Glu Glu Gly Leu Lys Asn
370 375 380
Ser Ala Gly Glu Leu Phe Thr Leu Gly Gln Met Met Thr Gly Trp His
385 390 395 400
Lys Thr Met Asp Glu Lys Gly Lys Glu Phe Trp Tyr Tyr Phe Ser Pro
405 410 415
Lys Glu Glu Gly Leu Lys Asn Phe Ala Gly Lys Leu Phe Thr Leu Gly
420 425 430
Gln Met Met Thr Gly Thr Val Lys Ile Gly Asn Lys Gly Tyr Thr Phe
435 440 445
Ala Glu Asn Gly Val Cys Thr Asn Pro Asn Ile
450 455
<210> 186
<211> 463
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 186
Met Ile Asp Asn Asn Asn Gln Thr Thr Asn Ile Met Asp Gln Phe Ser
1 5 10 15
Asp Leu Met Asn Glu Lys Ile Lys Asn Tyr Asn Arg Gln Asn Ile Lys
20 25 30
Ala Thr Leu Lys Ala Leu His His Glu Leu Lys Ala Leu Asp Asp Ser
35 40 45
Leu Asn Gly Lys Arg Val Ser Glu Ser Tyr Tyr Ala Ser Asp Ser Pro
50 55 60
Asp Glu His Asn Arg Asn Arg Val Arg Phe Ile His Ser Asn Phe Lys
65 70 75 80
Ala Leu Ile Leu Gln Cys Ser Lys Glu Gly Tyr Glu Glu Pro Glu Leu
85 90 95
Pro Leu Tyr Thr Ala Val Ala Thr Ala His Ile Leu Phe Leu Arg Cys
100 105 110
Ile Glu Lys His Gly Leu Gly Pro Lys Leu Gln Phe His Ile Ser Asp
115 120 125
Tyr Asn Glu Phe Met Lys Asp Leu Lys Asp Tyr Pro Asp Lys Tyr Ile
130 135 140
Lys His Ile Lys Glu Thr Tyr Pro Lys Gly Ser Ser Lys Leu Phe Lys
145 150 155 160
Glu Pro Thr Leu Val Pro Lys Tyr Tyr Ala Glu Thr Lys Gly Asn Lys
165 170 175
Ala Phe Trp Met Ala Val Lys Arg Asn Thr Leu Val Pro Gln Asn Gly
180 185 190
Lys Trp Tyr Tyr Leu Asp Arg Asp Gly Lys Met Lys Thr Gly Trp Val
195 200 205
Lys Thr Gly Gly Phe Ile Met Asp Asp Asn Gly Gln Leu Glu Met His
210 215 220
Gln Pro Asp Ala Tyr Tyr Tyr Phe Ser Ser Asn Gly Pro Met Val Thr
225 230 235 240
Gly Trp Phe Thr Asp Ala Asp Gly Arg Thr Tyr Tyr Leu Asn Pro Glu
245 250 255
Glu Thr Gln Lys Phe Tyr Lys Gly Gln Met Ala Glu Gly Phe Val Glu
260 265 270
Ile Gly Lys Gly Lys Trp Phe Tyr Phe Asn Pro Leu Asp Ile Ser Arg
275 280 285
Leu Ala Pro Asn Gly Tyr Asp Asn Leu Pro Glu Ala Gly Lys Lys Leu
290 295 300
Leu Lys Asp Thr Phe Gly Ile Ser Lys Asn Ile Leu Arg Gly Glu Gln
305 310 315 320
Arg Thr Glu Trp Ile His Asp Asn Gly Ala Trp Tyr Tyr Cys Ser Pro
325 330 335
Glu Lys Asn Asn Arg Phe Asp Lys Gly Gln Met Val Thr Gly Glu Val
340 345 350
Asn Ile Lys Asn Ser Glu Gly Asn Ile Lys Thr Tyr Gln Phe Asn Asn
355 360 365
Asp Gly Ala Cys Thr Asn Thr Tyr Gln Lys Met Val Leu Gln Tyr Phe
370 375 380
Met Val Val Val Pro Phe Phe Ile Ala Tyr Gln Leu Phe Tyr Thr Asn
385 390 395 400
Glu Tyr Leu Asn Leu Glu Ile Ile Phe Cys Pro Ser Lys Leu Phe Lys
405 410 415
Thr Phe Phe Asn Leu Gly Glu Leu Thr Tyr Tyr Phe Lys Arg Arg Ser
420 425 430
Ser Leu Tyr Leu Ser Asn Phe Cys Tyr Pro Thr Ile Thr Arg Ile Leu
435 440 445
Ile Leu Leu Tyr Ile Ile Thr Leu Leu Lys Phe Tyr Asn Tyr Leu
450 455 460
<210> 187
<211> 291
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 187
Met Asp Thr Thr Glu Lys Val Pro Gln Asp Val Ser Lys Ile Gln Glu
1 5 10 15
Gln Asn Lys Lys Gln Gln Gly Ile Gln Asp Trp Gln Gly Pro Ile Asp
20 25 30
Phe Thr Ile Gly Gln Phe Phe Gln Lys Lys Thr Gly Asn Asn Val Ala
35 40 45
His Ile Leu Thr Arg Tyr Asp Asn Pro Lys Phe Ile Glu Thr Thr Ala
50 55 60
Val Ala Ser Ser Ser Pro Lys Ile Thr Asp Leu Lys Glu Val Tyr Val
65 70 75 80
Gly Arg Ser Ile Leu Arg Asn Asn Thr Asp Ile Asp Gln Thr Leu Tyr
85 90 95
Thr Asp Ser Tyr Ser Glu Thr Ile Thr Glu Ser Thr Thr Ser Ala Val
100 105 110
Thr His Gly Phe Asn Ile Gly Glu Ser Thr Thr Ala Ser Leu Ser Leu
115 120 125
Pro Ile Val Gly Ala Gly Phe Glu Gln Glu Val His Met Glu Tyr Asn
130 135 140
Ile Ser Thr Glu Asn Ser Thr Thr Lys Glu Lys Ser Arg Thr Tyr Thr
145 150 155 160
Ala Pro Ser Gln Ala Ile Val Val Pro Ala His Ser Ser Val Glu Val
165 170 175
Ile Val Lys Leu Asp Arg Ile Lys Ala Asp Gly Lys Val Asp Leu Phe
180 185 190
Ser Lys Met Lys Asp Thr Gln Tyr Thr Ser Leu Val Trp Phe Lys Asp
195 200 205
Arg Pro Asp Gln Met Phe Val Gly Ser Val Tyr Asp Ala Val Arg Asp
210 215 220
Val Thr Ser Asp Gln Thr Phe Pro Asp Ile Lys Ala Asn Lys Lys Asp
225 230 235 240
Lys Thr Ile Ser Leu Ile Gly Lys Gly Asn Tyr Lys Ala Val Leu Gly
245 250 255
Thr Thr Phe Ser Val Asn Val Lys Pro Val Asn Lys Glu Gly Arg Ala
260 265 270
Ile Asn Asp Gly Tyr Thr Tyr Lys Val Asn Pro Gln Ile Thr Asp Val
275 280 285
Lys Ile Lys
290
<210> 188
<211> 575
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 188
Met Asp Gln Thr Ser Arg Leu Asp Asp Phe Ile Lys Asn Asp Glu Ala
1 5 10 15
Gly His Tyr Gly Lys Asn Arg Asp Gly Phe Tyr Ile Lys Ser Pro Asp
20 25 30
Glu Ala Arg Val Leu Pro Gln Gly Gly Ser Trp Phe Thr His Asp Thr
35 40 45
His Tyr Arg Asp Ala Glu Ile Ala Pro Phe Phe Leu Ile Asp Glu Ser
50 55 60
Lys Met Asp Ser Ile Ile Lys Glu Val Gln Asn Lys Lys Asp Met Glu
65 70 75 80
Leu Thr Asp Leu Leu Glu Ile Ile Thr Leu His Ala Pro Ser Lys Glu
85 90 95
Met Asp Gly Glu Asp Asn Asp Ala His Leu Val Arg Tyr Cys Arg Thr
100 105 110
Pro Gly Ile Asp Lys Asp Val Lys Asn His Glu Tyr Leu Thr Val Asp
115 120 125
Thr Gly Lys Ser Lys Ile Val Ser Thr Lys Ile Glu Lys Ala Asp Ala
130 135 140
Lys Asp Asp Val Asn Leu Leu Thr Asn Thr Phe Thr Asn Pro Thr Asp
145 150 155 160
Gly Pro Ile Gln Ala Thr Ser Gly Thr Ile Ser Lys Gln Phe Thr Asn
165 170 175
Thr Ile Ser Thr Thr Gln Thr Asn Ala Thr Thr Leu Gly Met Lys Gln
180 185 190
Thr Leu Lys Thr Gly Ile Asp Phe Phe Asp Ile Ala Lys Glu Asp Phe
195 200 205
Ser Leu Glu Phe Asn Glu Ser Tyr Thr Tyr Thr Asn Ala His Gln Thr
210 215 220
Leu Thr Ser Glu Ala Thr Thr Leu Thr Ser Gly Ser Val Ser Val Asn
225 230 235 240
Ile Lys Pro Gly Lys Ser Tyr Lys Val Gln Thr Met Met Arg Arg Asp
245 250 255
Lys Glu Ser Gly Ile Met Gln Gly Thr Ser Arg Leu Gly Gly Glu Tyr
260 265 270
Thr Ile Ser Asn Gly Asp Tyr Arg Val Asp Leu Asn Ile Tyr Lys Lys
275 280 285
Phe Lys Val Ile Gln Asp Phe Tyr Pro Lys Leu Trp Ala Val Leu Lys
290 295 300
Asp Arg Gly Ile Asp Leu Asp Asp Asn Thr Gln Glu Val Leu Tyr Thr
305 310 315 320
Gly Gly Ile Gly Phe Asp Ser Ile Arg Gly Ala Asn Ile Ile Thr Thr
325 330 335
Ile Thr Asp Leu Asp Thr Gly Asn Lys Ile Glu Lys Pro Val Ser Gln
340 345 350
Val Lys Ala Thr Gly Thr Ile Asn Leu Asp Gly Pro Ile Gln Ala Ser
355 360 365
Ile Gln Thr Gly Ile Ala Glu Leu Glu Lys Asn Ser Leu Lys Leu Gln
370 375 380
Thr Ile Asp Gly Val Thr Tyr Cys Val Asp Lys Asp Gly Asn His Leu
385 390 395 400
Thr Gly Trp Gln Thr Val Ser Gly Thr Gln Tyr Tyr Phe Gly His Asn
405 410 415
Gly Asp Gly Ser Gly Leu Ile His Glu Gly Glu Met Ala Lys Gly Trp
420 425 430
Lys Lys Leu Asp Gly Val Gln Tyr Tyr Phe Asn Lys Thr Gly Asp Gly
435 440 445
Ser Gly Met Thr His Glu Gly Glu Met Ala Lys Gly Phe Lys Thr Ile
450 455 460
Asp Gly Val Pro Tyr Tyr Phe Gly Gln Thr Gly Asp Gly Ala Gly Met
465 470 475 480
Asn Tyr Glu Gly Glu Met Ala Lys Gly Trp Lys Thr Ile Asp Gly Lys
485 490 495
Thr Tyr Tyr Phe Gly Gln Thr Gly Asp Gly Ala Gly Met Asn His Glu
500 505 510
Gly Gln Met Ala Thr Asp Trp Thr Thr Ile Asn Gly Val Lys Tyr Tyr
515 520 525
Phe Gly Lys Ala Gly Asp Gly Ile Ala Ser Leu Ala Val Gly Glu Arg
530 535 540
Ala Thr Gly Ser Met Met Ile Asp Gly Gln Val His Tyr Phe Asn Lys
545 550 555 560
Thr Gly Asp Gly Thr Gly Tyr His His Glu Gly Glu Leu Glu Trp
565 570 575
<210> 189
<211> 583
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 189
Met Asp Glu Lys Gln Thr Glu Val Gln Gln Gln Gln Ala Asp Ala Pro
1 5 10 15
Pro Ser Thr Gly Pro Val Phe Lys Lys Glu Lys Asp Gly Thr Phe Thr
20 25 30
Val Leu Lys Lys Asn Ile Phe Asn Met Ala Thr Leu Lys Ala Ile Gly
35 40 45
Ser Leu Gly Gly Ala Gly Leu Lys Gln Ala Tyr Ala Asp Tyr Gln Val
50 55 60
Asp Gly Gly Asn Phe Asn Asn Thr Phe Arg Thr Leu Ala Met Gly Ser
65 70 75 80
Ala Ala Leu Ile Pro Tyr Gly Gly Val Val Leu Ser Pro Leu Ile Gly
85 90 95
Leu Leu Trp Ser Glu Asp Lys Thr Ala Glu Ala Asn Gln Leu Lys Lys
100 105 110
Leu Met Glu Ser Ile Ala Ala Gln Thr His Glu Gln Ile Ala Lys Tyr
115 120 125
Asp Thr Ala Lys Leu Ser Lys Ala Leu Gln Asp Leu His Glu Lys Val
130 135 140
Glu Glu Phe Glu Asn Leu Val Pro Ile Pro Gly Ser Gly Ile Phe Tyr
145 150 155 160
Ser Gly Asp Gln Ile His Lys Tyr Glu Asn Ile Gln Ile Ser Ala Lys
165 170 175
Asn Leu Asn Asn Tyr Phe Arg Ser Ala Ile Ala Ala Cys Gln Thr Glu
180 185 190
Gly Tyr Lys Glu Ser Glu Leu Pro Ile Tyr Ile Ile Leu Ala Thr Met
195 200 205
His Met Asn Phe Met Asn Tyr Val Ile Lys Asn Gly Glu Asn Leu Leu
210 215 220
His Phe Asp Pro Glu Val Leu Lys Ser Glu Phe Thr Asp Lys Gln Asp
225 230 235 240
Gly Tyr Ile His Gly Val His Asn Val Pro Gly Gln Ser Glu Lys Val
245 250 255
Gly Tyr Ile Glu Tyr Ile His Asn Phe Ala Lys Lys Asp Leu Asp Leu
260 265 270
Thr Ser Asn Phe Glu Arg Phe Leu Leu Asp Tyr Lys Phe Lys Leu Asp
275 280 285
His Asp Lys Ala Leu Ser Ile Gly Ser Ser Pro Ala Lys Leu Ile Ser
290 295 300
Ser Ile Val Met Asn His Asn Asn Lys Leu Glu Lys Thr Leu Asn Asn
305 310 315 320
Ser Ile Asn Asn Ile Ala Phe Lys Gln Ala Ala Asn Ile Lys Ser Glu
325 330 335
Phe Thr Leu Asp Gln Asn Gly Arg Pro Leu Tyr Tyr Ser Leu Asp Gly
340 345 350
Lys Met Gln Thr Gly Trp Lys Glu Ile Gly Thr Ser Phe Ala Thr Arg
355 360 365
His Asp Thr Gly Ser Ser Thr Ala Glu Arg Leu Phe Ser Arg Glu Trp
370 375 380
Leu Lys Ala Gln Gly Ile Gln Tyr Ser Trp Tyr Tyr Phe Ser Pro Thr
385 390 395 400
Lys Thr Asp Lys Phe Glu Gln Gly Glu Met Tyr Val Asp Thr Thr Gln
405 410 415
Thr Leu Pro Asp Gly Lys Thr Tyr Thr Phe Asp Ser Asp Gly Lys Cys
420 425 430
Leu Asn Pro Asp Gly Gly Ala Val Asn Val Gln Ile Leu Lys Glu Thr
435 440 445
Tyr Lys Ile Val Ser Lys Leu Asp Asn Asn Met Val Leu Asn Thr Ser
450 455 460
Asp His Gln Ala Val Leu Lys Gly Thr Asn Glu Asp Thr Trp Gly Asn
465 470 475 480
Ser Trp Thr Phe Gln Tyr Asp Asn Glu Lys His Ala Tyr Leu Ile Thr
485 490 495
Asn Gly Gln Ser Gly Gly Pro Trp Lys Leu Ala Glu Tyr Ser Asn Thr
500 505 510
Asn Thr Val Phe Ala Thr Asp Asp Lys Ala Asn Lys Gln Glu His Tyr
515 520 525
Trp Thr Leu Glu Pro Gly Gly Asp Gly Tyr Phe Phe Ile His Asn Lys
530 535 540
Ser Gln Pro Asp Lys Val Leu Asp Ile Thr Gly Ser Asn Arg Ala Ala
545 550 555 560
Gly Thr Lys Ile Gly Leu Phe Asn Lys Asn Gly Gln Asp Asn Gln Lys
565 570 575
Phe Lys Leu Glu Lys Ile Asn
580
<210> 190
<211> 1030
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 190
Met Ala His Leu Ser Asp Phe Tyr Pro Ile Ser Tyr Asn Val Leu Ser
1 5 10 15
Gln Lys Ser Val Tyr Asn Asp Asn Glu Met Tyr Glu Val Gln Gln Ile
20 25 30
Phe Lys Asn Val Leu Thr Ser Trp Glu Gln Tyr Ser Gln Asp Gly Arg
35 40 45
Ile Ser Thr Gln Gln Gln Glu Leu Lys Lys Ala Ile His Asn Leu Lys
50 55 60
Lys Tyr Asn Leu Asp Leu Phe Asp Leu Phe Ile Asn Ile Leu Pro Leu
65 70 75 80
Gly Ala Thr Ile Ser Pro Leu Pro Pro Val His Ser Asn Asp Val Met
85 90 95
Ile Ser Thr Ile Ile Pro Asn Ile Cys Phe Ile Val Asp Ile Val Trp
100 105 110
Pro Gln Ile Pro Lys Gly Leu Asn Thr Gln Leu Asn Met Trp Glu Val
115 120 125
Ile Lys Asp Arg Val Lys Lys Ile Thr Asn Gln Lys Ile Glu Ile Asn
130 135 140
Asn Tyr Glu Gln Ile Leu Thr Thr Leu Asn Gln Val Lys Ile His Leu
145 150 155 160
Leu Asp Leu Lys Asn Ala Ile Gln Thr Thr Val Asp Gln Ser Leu Ser
165 170 175
Tyr Gln Asn Ile Thr Ala Asp Asn Asn Val Tyr His Pro Tyr Lys Asp
180 185 190
Gln Glu Phe Asn Ala Ile Ile Arg Ile Gln Leu Ala Asn Val Leu Ala
195 200 205
Gln Phe Asp Gln Tyr Ile Ser Ile Phe Gln Met Asn Asn Cys Val Ile
210 215 220
Ile Glu Leu Pro Tyr Phe Cys Leu Leu Ala Thr Leu His Leu Phe Thr
225 230 235 240
Leu Gln Ser Val Val His Phe Gly Ser Gln Trp Gly Tyr Ser Thr Met
245 250 255
Gln Ile Lys Asp Tyr Ser Lys Lys Leu Gln Gln Lys Ile Lys Glu Tyr
260 265 270
Thr Asn His Val Tyr Ser Ile Cys Ile Asn Pro Ser His Ile His Arg
275 280 285
Leu Asn Asp Ser Lys Asn Ser Leu Tyr Lys Leu Asp Ser Leu Phe Ser
290 295 300
Asn Met Ile Asn Glu Cys Cys Asn Tyr Ile Ala Ile Trp Pro Leu Leu
305 310 315 320
Asp Pro Val Asp Tyr Pro Ile Arg Thr His Leu Glu Gln Ile His Ile
325 330 335
Pro Phe Ser Asn Ile Ile Thr Ser Glu Asn Ala Thr Phe Glu Leu Asn
340 345 350
Asn His Ile Gln Lys Lys Thr Phe Gln Ser His Asn Leu Ser Lys Lys
355 360 365
Thr Asn Leu Asn Lys Ile Ala Thr Ile Gly Glu Val Asp Arg Asn Ser
370 375 380
Gly Val Ile Met Ile Ala Gly Val Pro Val Val Ile Gly Ile Thr Asn
385 390 395 400
Gly Gln Tyr Val Thr Glu His Ile Thr Lys Thr Ser Ala Ile Lys Leu
405 410 415
Ser Lys Asn Gln Ile Ile Glu Leu Pro Phe Phe Asn Leu Thr Thr Gly
420 425 430
Leu Tyr Gln Ile Arg Tyr Arg Leu Ser Thr Thr Glu Ser Gly Phe Leu
435 440 445
His Phe Lys Leu Gly Arg Trp Asp Asn Ile Val Ser Leu Glu Gln Ile
450 455 460
Glu Thr Tyr Asn His Ser Thr Leu Asp Phe Gly Gly Lys Pro Gly Ile
465 470 475 480
Tyr Lys Leu Pro Ser Lys Lys Val Thr Pro Arg Cys Gly Asn Leu Ser
485 490 495
Ser Ile Asp Asn Ile Leu Ala Ile Asn Ser Gly His His Ile Leu Thr
500 505 510
Ile Ser Asn Ile Gly Ser Val Asn Ile Val Val Asp Arg Ile Glu Phe
515 520 525
Ile Tyr Ile Ser Asn Met Asp Ser Pro His Ser Pro Met Val Phe Asn
530 535 540
Asn Lys Lys Tyr Leu Glu Lys Ile Lys Arg Gln Val Asn Gln Leu Phe
545 550 555 560
Thr Asp Thr Leu Lys Asn Lys Leu Lys Lys Glu Ile Ser Asp Tyr His
565 570 575
Ile Asp Gln Ile Ala Leu Lys Ile Asp Lys Leu Ser Asn Lys Ala Phe
580 585 590
Gln Leu Glu Lys Lys Tyr Leu Arg Gln Leu Ile Asn Lys Ala Lys Arg
595 600 605
Leu Ser Lys Glu Arg Asn Lys Leu Val Gly Gly Asn Phe Glu Lys Leu
610 615 620
Asp Lys Trp Leu Leu Gly Tyr Asp Val Val Lys Lys Tyr Asn Lys Lys
625 630 635 640
Ile Cys Lys Gly Glu Tyr Leu Leu Leu Pro Pro Pro Glu Lys Gly Leu
645 650 655
Ser Tyr Ile Tyr Gln Lys Val Asp Glu Ser Lys Leu Lys Pro Tyr Thr
660 665 670
Arg Tyr Ile Ile Ser Gly Tyr Ile Ile Gln Ser Glu Asn Leu Glu Leu
675 680 685
Ile Val Ser Arg Tyr Gly Asn Glu Ile Gln Thr Leu Leu Asn Val Pro
690 695 700
His Ile Asn Glu Asn Thr Phe His Asn Gln Lys His Val Asn Ser His
705 710 715 720
Phe Phe Ser Tyr Thr Ile Asp Val Gly Met Leu His Pro Glu Ile Asn
725 730 735
Leu Gly Ile Glu Leu Gly Leu Arg Ile Val His Pro Tyr Gly Phe Ala
740 745 750
Lys Leu Ser Asn Leu Glu Ile Arg Glu Ser His Ser Leu Thr Asn Tyr
755 760 765
Asn Thr Leu Lys Arg Gln Asp Ser Leu Tyr Val Asn Lys Tyr Gln Asn
770 775 780
Gln Asn Arg Gly Leu Tyr Arg Asn Pro Thr Tyr Gln Ile Asn Asn Met
785 790 795 800
Lys Ile Lys Asp Asn His Ser Leu Thr Met Gln Glu Ile Ile Gln Lys
805 810 815
Glu Thr Ile Trp Lys Asn Lys Phe Asp Asn Glu Asn Lys Lys Phe Ile
820 825 830
Ile Asp Leu Thr Ser Ile Ile Asn Gln Ile Asn Thr Phe Tyr Lys Asp
835 840 845
Leu Asn Trp Asn Asn Thr Ile His Pro His Val Thr Tyr Gln Lys Ile
850 855 860
Asn Gln Val Lys Leu Pro Lys Leu Ser Asn Leu Lys His Trp Phe Met
865 870 875 880
Asn Asp Arg Pro Gly Glu His Tyr Lys Ile Ile Gln Thr Leu Gln Asn
885 890 895
Ala Leu Asp Cys Ala Phe Ile Gln Leu Glu Asn Gln Asn Leu Ile Arg
900 905 910
Asn Gly Gly Phe Thr Asn Gly Leu Glu Asn Trp Thr Ile Gln Gly Gln
915 920 925
Val Lys Ile Val Thr Phe Ile Pro Asn Lys Gln Thr Val Gln Leu Ser
930 935 940
Cys Trp Asp Ala Tyr Met Val Gln Thr Ile Asn Ile Lys Glu Phe Asp
945 950 955 960
Thr Ile His Thr Tyr Lys Leu Arg Val Tyr Gly Lys Gly Thr Gly Asn
965 970 975
Ile Val Ile Gln His Ser Asp Tyr Val Glu Leu Met Ser Phe Ser Thr
980 985 990
Asn Glu Phe Ile Phe Lys Glu Leu Leu Phe Ser Leu Lys Thr Asn Ile
995 1000 1005
Ile Glu Val Leu Ile Gln Ser Glu Gly Pro Lys Phe Phe Ile Asp
1010 1015 1020
Ser Ile Glu Ile Phe Lys Tyr
1025 1030
<210> 191
<211> 531
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 191
Met Ala His Leu Ser Asp Phe Tyr Pro Ile Ser Tyr Asn Val Leu Ser
1 5 10 15
Gln Lys Ser Val Tyr Asn Asp Asn Glu Met Tyr Glu Val Gln Gln Ile
20 25 30
Phe Lys Asn Val Leu Thr Ser Trp Glu Gln Tyr Ser Gln Asp Gly Arg
35 40 45
Ile Ser Thr Gln Gln Gln Glu Leu Lys Lys Ala Ile His Asn Leu Lys
50 55 60
Lys Tyr Asn Leu Asp Leu Phe Asp Leu Phe Ile Asn Ile Leu Pro Leu
65 70 75 80
Gly Ala Thr Ile Ser Pro Leu Pro Pro Val His Ser Asn Asp Val Met
85 90 95
Ile Ser Thr Ile Ile Pro Asn Ile Cys Phe Ile Val Asp Ile Val Trp
100 105 110
Pro Gln Ile Pro Lys Gly Leu Asn Thr Gln Leu Asn Met Trp Glu Val
115 120 125
Ile Lys Asp Arg Val Lys Lys Ile Thr Asn Gln Lys Ile Glu Ile Asn
130 135 140
Asn Tyr Glu Gln Ile Leu Thr Thr Leu Asn Gln Val Lys Ile His Leu
145 150 155 160
Leu Asp Leu Lys Asn Ala Ile Gln Thr Thr Val Asp Gln Ser Leu Ser
165 170 175
Tyr Gln Asn Ile Thr Ala Asp Asn Asn Val Tyr His Pro Tyr Lys Asp
180 185 190
Gln Glu Phe Asn Ala Ile Ile Arg Ile Gln Leu Ala Asn Val Leu Ala
195 200 205
Gln Phe Asp Gln Tyr Ile Ser Ile Phe Gln Met Asn Asn Cys Val Ile
210 215 220
Ile Glu Leu Pro Tyr Phe Cys Leu Leu Ala Thr Leu His Leu Phe Thr
225 230 235 240
Leu Gln Ser Val Val His Phe Gly Ser Gln Trp Gly Tyr Ser Thr Met
245 250 255
Gln Ile Lys Asp Tyr Ser Lys Lys Leu Gln Gln Lys Ile Lys Glu Tyr
260 265 270
Thr Asn His Val Tyr Ser Ile Cys Ile Asn Pro Ser His Ile His Arg
275 280 285
Leu Asn Asp Ser Lys Asn Ser Leu Tyr Lys Leu Asp Ser Leu Phe Ser
290 295 300
Asn Met Ile Asn Glu Cys Cys Asn Tyr Ile Ala Ile Trp Pro Leu Leu
305 310 315 320
Asp Pro Val Asp Tyr Pro Ile Arg Thr His Leu Glu Gln Ile His Ile
325 330 335
Pro Phe Ser Asn Ile Ile Thr Ser Glu Asn Ala Thr Phe Glu Leu Asn
340 345 350
Asn His Ile Gln Lys Lys Thr Phe Gln Ser His Asn Leu Ser Lys Lys
355 360 365
Thr Asn Leu Asn Lys Ile Ala Thr Ile Gly Glu Val Asp Arg Asn Ser
370 375 380
Gly Val Ile Met Ile Ala Gly Val Pro Val Val Ile Gly Ile Thr Asn
385 390 395 400
Gly Gln Tyr Val Thr Glu His Ile Thr Lys Thr Ser Ala Ile Lys Leu
405 410 415
Ser Lys Asn Gln Ile Ile Glu Leu Pro Phe Phe Asn Leu Thr Thr Gly
420 425 430
Leu Tyr Gln Ile Arg Tyr Arg Leu Ser Thr Thr Glu Ser Gly Phe Leu
435 440 445
His Phe Lys Leu Gly Arg Trp Asp Asn Ile Val Ser Leu Glu Gln Ile
450 455 460
Glu Thr Tyr Asn His Ser Thr Leu Asp Phe Gly Gly Lys Pro Gly Ile
465 470 475 480
Tyr Lys Leu Pro Ser Lys Lys Val Thr Pro Arg Cys Gly Asn Leu Ser
485 490 495
Ser Ile Asp Asn Ile Leu Ala Ile Asn Ser Gly His His Ile Leu Thr
500 505 510
Ile Ser Asn Ile Gly Ser Val Asn Ile Val Val Asp Arg Ile Glu Phe
515 520 525
Ile Tyr Ile
530
<210> 192
<211> 361
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 192
Met Ala Thr Leu Asn Leu Ser Glu Glu Ser Lys Lys Phe Gln Ser Ile
1 5 10 15
Thr Phe Ala Glu Ser Thr Glu Gln Tyr Gly Leu Arg Gln Tyr Pro Asn
20 25 30
Leu Asn Glu Asn Pro Thr Asn Arg Val Phe Asn Asp Ala Tyr His Lys
35 40 45
Glu Leu Ala Asp Gln Tyr Gly Phe Ile Phe Tyr Lys Leu Asp Ala Asp
50 55 60
Asp Gln Phe Ile Ile Ala Asn Arg Asn Thr Gly Glu Val Leu Gln Ala
65 70 75 80
Pro Gly Thr Thr Gly Pro Asp Ser Val Val Leu His Lys Gln Tyr Asp
85 90 95
Ala Ser Ile Gly Gln Lys Ile Thr Lys Lys Gln Leu Thr Lys Asp Asn
100 105 110
Phe Gln Leu Tyr Val Tyr Asn Ser Thr Gly Ile Ile Ser Ala Pro Tyr
115 120 125
Asp Lys Val Gly Glu Trp Val Asn Ile Val Ser Leu Pro Lys Asn Ser
130 135 140
Asn Glu Asn Asn Val His Thr His Leu Lys Asn Arg Thr Asp Ile Lys
145 150 155 160
Phe Glu Ile Asn Ile Pro Ser Leu Ser Pro Thr Thr Lys Leu Glu Pro
165 170 175
Ile Pro Glu Leu Pro Gly Phe Asn Asp Pro Leu Pro Glu Asn Glu Asp
180 185 190
Ala Lys Arg Ala Val Ile Gly Ser Ala Leu Ile Pro Cys Val Leu Val
195 200 205
Asn Asp Ser Ile Ser Leu Ala Asp Lys Ile Lys Glu Tyr Pro Tyr Tyr
210 215 220
Val Leu Glu Tyr Arg Gln Tyr Trp His Leu Leu Trp Ser Asp Val Phe
225 230 235 240
Thr Ala Gly Ser Asn Lys Ile Lys Thr Glu Val Thr Gly Ile Pro Ser
245 250 255
Glu Ala Gln Glu Lys Met Lys Glu Phe Val Gly Ala Ser Ile Arg Thr
260 265 270
Asp Ile Gly Leu Arg Phe Asn Glu Lys Ser Glu Pro Phe Gly Lys Gln
275 280 285
Ile Thr Ser Gly Leu Asn Val Lys His Ser Tyr Asp Tyr Glu Leu Gly
290 295 300
Glu Thr Thr Thr Thr Thr Asn Val Asp Asn Pro Asn Asp Phe Thr Val
305 310 315 320
Arg Tyr Ala Arg Tyr Ala Arg Ala His Glu Phe Val Leu Lys Arg Ser
325 330 335
Asp Gly Gln Val Val Glu Glu Pro Trp Val Ala Ile Asp Glu Asn Asp
340 345 350
Met Tyr Leu Lys Met Tyr Lys Arg Ala
355 360
<210> 193
<211> 337
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 193
Met Ala Glu Ser Leu Ser Ser Pro Val Ser Phe Thr Asn Ile Tyr Asn
1 5 10 15
Asp Gly Ala Asp Tyr Thr Lys Glu Asn Leu Ala Lys Asp Leu Arg Lys
20 25 30
Arg Ile Leu Trp Gly Ile Glu Glu Thr Pro Glu Leu Arg Gln Lys Phe
35 40 45
Lys Leu Ala Asp Asp Glu Ser Ile Gln Arg Glu Asn Pro Ala Thr Ser
50 55 60
Phe Thr Asp Pro Asn Gly Asn Thr Thr Glu Tyr Pro Ala Leu Tyr Tyr
65 70 75 80
Gly Leu Glu Asp Gln Leu Ser Lys Val Glu Val Gly Val Val Gly Ser
85 90 95
His Phe Glu Val Asn Pro Thr Val Asp Ser Tyr Glu Asp Leu Gly Gln
100 105 110
Thr Asn Leu Leu Thr Ile Asn Asn Asp Asp Gly Thr Val Ala Gln Thr
115 120 125
Ala Gln Thr Pro Glu Thr Thr Glu Lys Phe Ser Glu Thr Met Ser Tyr
130 135 140
Ser Asn Ser Thr Gly Met Lys Ile Gly Ile Gly Thr Ser Thr Thr Ile
145 150 155 160
Lys Ile Lys Ile Pro Phe Asp Leu Gly Glu Ala Ser Gln Thr Val Asn
165 170 175
Met Asn Ser Glu Phe Ser Phe Asn Asn Thr Gln Thr Gln Thr Ser Thr
180 185 190
His Glu Lys Ser Val Thr Phe Lys Ser Gln Pro Val Val Ala Ala Pro
195 200 205
Gly Gly Thr Thr Thr Tyr Tyr Gly Thr Ile Lys Arg Ala Lys Phe Ser
210 215 220
Gly Thr Phe Gln Thr Asp Ala Tyr Val Pro Gly Leu Thr Leu Lys Leu
225 230 235 240
Pro Ile Val Lys Lys Asn Asn Gly Asn Asp Ile Val His Thr Glu Glu
245 250 255
Val Thr Leu Thr Pro Glu Asp Met Tyr Ala Ile Phe Lys Asn Gly Leu
260 265 270
Pro Val Leu Pro Pro Tyr Leu Ser Leu Asp Asp Lys Ile Lys Lys Val
275 280 285
Lys Val Asn Asn Ala Ser Phe Thr Phe Asn Gly Glu Gly Gly Tyr Tyr
290 295 300
Ser Ala Val Gln Val Lys Phe Ile Pro Lys Asp Pro Asn Lys Lys Ala
305 310 315 320
Gln Val Met Pro Tyr Lys Glu Tyr Val Ala Lys Thr Gln Glu Lys Ser
325 330 335
Leu
<210> 194
<211> 333
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 194
Met Ala Glu Thr Gln Leu Asn Val Gly Gln Lys Ala Val Thr Pro Asp
1 5 10 15
Val Ser Ser Tyr Ile Asp Ile Val Gln Asp Arg Met Lys Gln Arg Asp
20 25 30
Lys Glu Ser Lys Leu Ser Gly Glu Ala Ile Asn Met Gln Glu Gln Ile
35 40 45
Ile Asp Gly Trp Phe Leu Ser Arg Phe Trp Ile Phe Lys Asp Gln Asp
50 55 60
Asn Asn His Gln Thr Asn Arg Phe Ile Ser Trp Phe Lys Asp Asn Leu
65 70 75 80
Ala Ser Pro Lys Gly Tyr Asp Gln Ile Ala Glu Gln Met Gly Leu Asn
85 90 95
Ile Glu Ala Leu Asn Asp Met Asp Thr Ser Ser Ile Asn Tyr Thr Ser
100 105 110
Lys Ile Gly Asp Thr Ile Tyr Asn Gly Ile Ser Glu Leu Thr Asn Asn
115 120 125
Thr Gly Thr Thr Gln Lys Met Lys Thr Asp Ser Phe Gln Arg Asp Tyr
130 135 140
Thr Lys Ser Glu Thr Thr Ser Val Thr Asn Gly Leu Gln Val Gly Phe
145 150 155 160
Lys Val Leu Ala Lys Gly Ile Ile Tyr Leu Ala Gly Ala Asp Leu Glu
165 170 175
Ala Ser Val Asn Tyr Asn Leu Ser Thr Thr Thr Ser Leu Thr Asn Thr
180 185 190
Val Ser Asp Lys Phe Thr Val Pro Ser Gln Glu Val Thr Leu Pro Pro
195 200 205
Gly His Lys Ala Val Ile Lys His Asn Leu Asn Lys Met Val Tyr Ser
210 215 220
Gly Ser His Asp Leu Lys Gly Asp Leu Lys Val Ser Phe Ser Asp Lys
225 230 235 240
Glu Leu Val Gln Lys Phe Ile Tyr Pro Asn Tyr Arg Glu Ile Asn Leu
245 250 255
Ser Asp Ile Arg Lys Thr Met Asn Glu Ile Asp Glu Val Asn Asn Val
260 265 270
Asn Pro Val Asp Phe Tyr Gln Leu Val Gly Val Lys Asn His Val Lys
275 280 285
Asn Gly Asp Thr Ile Tyr Ile Asp Thr Pro Ala Glu Phe Thr Phe Asn
290 295 300
Gly Ala Asn Pro Tyr Tyr Arg Ala Ile Phe Thr Glu Tyr Asp Asp Asn
305 310 315 320
Gly Asn Pro Ile Gln Val Lys Ser Leu Gly Gly Asn Leu
325 330
<210> 195
<211> 370
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 195
Met Ser Asn Glu Asn Asn Asn Arg Arg Asn Met Asn Ala Ser Met Gly
1 5 10 15
Ser Asp Gly Glu Ser Ile Gln Asn Lys Ile Gly Asp Ser Arg Ser Gln
20 25 30
Ile Ser Phe Pro Asp Phe Asn Phe Tyr Lys Ile Arg Thr Phe Ser Gly
35 40 45
Lys Tyr Ala Asp Ile Thr Gly Asp Ser Leu Leu Val Gln Tyr Asn Gly
50 55 60
His Asn Gly Asp Asn Gln Lys Phe Leu Ile Val Thr Leu Asp Asn Asp
65 70 75 80
Tyr Ser Ile Ile Ala Ala Lys His Thr Gly Lys Val Met His Glu Phe
85 90 95
Tyr Leu Phe Gly Thr Gly Arg Phe Leu Gln Ala Asp Phe His Asn Met
100 105 110
Asp Glu Gln Lys Phe Phe Ile Ser Asp Asp Gly Thr Ile Ala Val Lys
115 120 125
Lys Asp Arg Lys Val Trp Asp Ala Thr Gly Gly Thr Ser Asn Gly Ser
130 135 140
Pro Ile Ile Pro Tyr Asp Tyr His Gly Ala Ser Asn Gln Lys Phe Thr
145 150 155 160
Leu Glu Arg Ser Gly Ser Val Ser Val Thr Lys Pro Thr Ile Gly Thr
165 170 175
Leu Pro Pro Ala Pro Asp Phe Lys Thr Thr Asp Leu Asn Glu Ile Leu
180 185 190
Pro Asp Asn Thr Glu Pro Val Ile Thr His Ala Thr Tyr Leu Pro Tyr
195 200 205
Phe Met Val Lys Asp Pro Tyr Tyr Asn Asn Gln Gln Lys Met Gln Asn
210 215 220
Ser Pro Tyr Tyr Ile Leu Val Arg Arg Gln Tyr Trp Glu Lys Ile Thr
225 230 235 240
Gln Arg Ile Val Gly Ala Gly Glu Ser Tyr Lys Tyr Glu Gln Lys Thr
245 250 255
Gly Val Ser Arg Thr Asp Gln Thr Ser Met Thr Glu Thr Thr Glu Ile
260 265 270
Ala Ile Gly Ala Asp Met Gly Phe Met Phe Lys Gly Phe Ser Val Gly
275 280 285
Leu Ser Thr Ser Ile Thr Lys Thr Leu Ser Val Thr Lys Ser Thr Ser
290 295 300
Thr Thr Glu Ser Thr Glu Thr Thr Glu Ile Val Thr Asp Ser Asn Pro
305 310 315 320
Phe Met His Thr Ile Ala Arg Ala Lys Tyr Met Leu Ile Asn Glu Tyr
325 330 335
Tyr Val Thr Arg Ala Asn Gly Glu Lys Ile Thr Ser Gly Asp Ala Ala
340 345 350
Tyr Trp Lys Val Pro Asn Pro Ser Gln Thr Ala Thr Arg Thr Ile Pro
355 360 365
Arg Thr
370
<210> 196
<211> 241
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 196
Met Arg Asn Tyr Thr Ser Glu Lys Gln Ser Gln Thr Val Leu Phe Ser
1 5 10 15
Glu Lys Ser Ile Glu Thr Phe Ser Arg Ser Thr Thr Glu Gly Tyr Lys
20 25 30
Val Gly Ser Ser Ile Lys Ser Thr Thr Ser Phe Lys Val Lys Val Gly
35 40 45
Phe Leu Val Ser Gly Glu Ile Ser Glu Ser Ile Glu Val Ala Val Thr
50 55 60
Gly Glu Tyr Asn His Ser Ser Thr Glu Thr Thr Thr Thr Thr Thr Thr
65 70 75 80
Thr Thr Glu Lys Leu Trp Gln Val Thr Gln Pro Val Ile Ile Pro Pro
85 90 95
His Thr Gln Ile Thr Ala Thr Leu Gln Ile Phe Ser Gly Pro Phe Val
100 105 110
Val Pro Ala Lys Thr Lys Ala Thr Ile Gln Gly Lys Gly Thr Ile Asn
115 120 125
Gly Thr Pro Asn Trp Ala Ser Phe Ile Asn Tyr Thr Lys Tyr Asp Gly
130 135 140
Arg Tyr Trp Ala Ala Tyr Asn Arg Ala Ser Gly Leu Tyr Asn Asp Arg
145 150 155 160
Asn Ser Trp Pro Gly Tyr Lys Lys Ile Tyr Val Gly Thr Gly Ser Gly
165 170 175
Thr Gly Ser Leu Arg Leu Glu Gly Glu Ala Arg Ile Asn Ala Gln Val
180 185 190
Gly Leu Tyr Ala Val Thr Glu Phe Lys Glu Ser Pro Leu Pro Gly Tyr
195 200 205
Glu Ser Val Gly Ser Asn Arg Thr Tyr Tyr Ala Pro Asn Ile Ile Leu
210 215 220
Gly Asp Gly Ser Ile Ile Gln Phe Pro Glu Tyr Gln Arg Tyr Leu Gln
225 230 235 240
Arg
<210> 197
<211> 297
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 197
Met Asp Thr Met Pro Gln Ala Lys Val Ala Pro His Val Thr Asn Phe
1 5 10 15
Ala Ala Ala Asp His Tyr Gln Gln Phe Met Asp Phe Met Ser Ser Lys
20 25 30
Gly Met Glu Arg Tyr Ala Asn Asn Phe Glu Ile Val Ala Met Pro Asp
35 40 45
Ser Gly Pro Lys Asn Ile Arg Ile Thr Glu Ala Lys Pro Ile Asn Ala
50 55 60
Pro Glu Asn Thr Thr Gln Val Met Ser Tyr His Asn Ser Thr Gly Val
65 70 75 80
Gln Gln Ser Leu Lys Thr Pro Ser Lys Lys Ile Thr His Lys Asn Thr
85 90 95
Ile Ala Ile Lys Asn Gly Gly Ser Ile Lys Thr Thr Ile Glu Ser Lys
100 105 110
Thr Thr Phe Gly Val Gln Val Pro Phe Phe Ala Lys Gly Glu Gln Glu
115 120 125
Leu Lys Val Gly Leu Glu Val Thr Tyr Asn Arg Glu Ser Thr Asp Thr
130 135 140
Ala Glu Asn Ile Gln Glu Phe Thr Val Pro Glu Gln Thr Leu Leu Ala
145 150 155 160
Gln Pro Gly Gly Thr Thr Arg Leu Ile Tyr Thr Val Lys Ser Thr Ala
165 170 175
Phe Ser Gly Thr Tyr Thr Asn Lys Ala Ile Phe Lys Gln Lys Gln Asp
180 185 190
Gly Ile Ser Lys Lys Phe Glu Asn Gly Lys Glu Ile Glu Gly Lys Glu
195 200 205
Leu Glu Ser Trp Asn Glu Thr Gln Gly Tyr Arg Phe Ile Lys Gln Ile
210 215 220
Ile Ala Ser Gln Pro Gln Leu Ala Asp Ile Phe Glu Met Asp Asp Ala
225 230 235 240
Asn Glu Lys Ala Tyr Leu Lys Glu Ala Val Phe Thr Phe Glu Gly Gln
245 250 255
Ala Gly His Gln Ser Thr Ala Glu Ala Val Phe Ile Pro Asp Asp Asn
260 265 270
Lys Lys Ser Glu Val Lys Met Pro Ile Ala Glu Tyr Asn Lys Arg Val
275 280 285
Ala Asn Gly Ile Ser Leu Tyr Ala Lys
290 295
<210> 198
<211> 287
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 198
Met Ile His Asp Leu Lys Glu Asp Ile Leu Asn Phe Thr Lys Trp Tyr
1 5 10 15
Ala Glu Lys His Tyr Asn Ala Asn Pro Asp Thr Phe His Asp Glu Lys
20 25 30
Val Phe Asn Pro Ser Val Ser Asn Thr Glu Val Val Pro Asp Lys Ile
35 40 45
Glu Phe Asp Thr Thr Pro Lys Leu Thr Ala Arg Ala Thr Asp Ile Leu
50 55 60
Ile Asn Asp Thr Ser Val Ser Gln Ser Ile Thr Pro Lys Ile Ser Thr
65 70 75 80
Lys Thr Val Glu Thr Thr Ser Ile Thr Thr Thr Glu Gly Tyr Lys Ile
85 90 95
Gly Ala Gly Ile Lys Tyr Thr Ser Thr Met Lys Leu Lys Leu Phe Leu
100 105 110
Ile Gly Glu Ile Ser Asn Ser Ile Glu Ile Ala Val Thr Ser Glu Tyr
115 120 125
Asn His Ser Thr Ser Glu Thr Gln Thr Asn Thr Thr Glu Lys Ile Trp
130 135 140
Glu Tyr Thr Arg Pro Val Ile Val Pro Pro Arg Thr Lys Val Thr Ala
145 150 155 160
Thr Leu Glu Val Tyr Ala Gly Pro Val Lys Val Pro Val Thr Leu Arg
165 170 175
Ser Thr Ile Thr Gly Met Gly Ile Ala Ser Thr Gly Tyr Gly Tyr Ile
180 185 190
Leu Gly Ser Val Ala Tyr Lys Asp Arg Asp Gly Lys Asn Trp Val Asp
195 200 205
Ser Trp Arg Ser Arg Ser Leu Tyr Asp Phe Lys Ser Thr Trp Pro Gly
210 215 220
Phe Lys Pro Val Tyr Val Gly Thr Asp Gly Glu Tyr Gly Val Lys Val
225 230 235 240
Gln Gly Thr Ala Gln Val Glu Leu Glu Leu Gly Leu Tyr Gly Val Val
245 250 255
Thr Tyr Gln Glu Glu Pro Leu Leu Gly Asn Asp Ser Asn Lys Ser Gln
260 265 270
Thr Tyr Arg Glu Ile Met Ser Arg Gln Gly Leu Tyr Thr Arg Ile
275 280 285
<210> 199
<211> 307
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 199
Met Lys Pro Ser Gln Thr Tyr Ala Glu Ser Asn Ser Met Gln Glu Lys
1 5 10 15
Thr Ile Gly Ile Thr Asn Val Asn Gln Val Leu Glu Lys Ile Gly Asn
20 25 30
Tyr Tyr Tyr Gln Arg Asn Leu Ser Leu Thr Trp Tyr Glu Ala Pro Asn
35 40 45
Ser Ile Gly Val Lys His Asn Phe Gln Leu Arg Lys Asn Ser Asp Ser
50 55 60
Ile Ser Ser Glu Ile Asp Leu Ser Ile Ser Gly Glu Glu Ile Glu Ser
65 70 75 80
Leu Tyr Tyr Asp Ser Thr Ile Pro Lys Phe Leu Gly Glu Asn Val Phe
85 90 95
Glu Asn Asn Thr Asp Gln Glu Gln Ser Tyr Asn Thr Ser Lys Phe Ser
100 105 110
Glu Thr Tyr Thr Glu Ser Thr Ser Thr Ser Val Ser Lys Gly Phe Lys
115 120 125
Ile Asn Val Gly Arg Asp Phe Thr Ile Pro Leu Ile Leu Asn Glu Gly
130 135 140
Gly Lys Ile Asn Leu Glu Tyr Asn Ser Gly Ser Thr Asn Thr Asn Thr
145 150 155 160
Leu Ser Lys Thr Tyr Thr Leu Glu Val Pro Ser Gln Pro Val Lys Val
165 170 175
Pro Pro Asn Lys Ile Tyr Lys Ser Val Val Glu Tyr Ser Gln Arg Thr
180 185 190
Tyr Lys Gly Thr Val Lys Phe Tyr Gly Arg Asn Leu His Asn Pro His
195 200 205
Pro Ile Asn Thr Ile Lys Thr Thr Gly Ser Tyr Thr Gly Trp Met Gly
210 215 220
Met Gln Glu Ile Lys Gln Phe Thr Phe Asn Asp Pro Leu Tyr Lys His
225 230 235 240
Tyr Asp Gly Leu Ser Asp Ser Gln Lys Lys Glu Val Glu Asn Asp Gly
245 250 255
Val Val Ile Ile Pro Phe Leu Gly Ala Thr Tyr Val Val Val Glu Gly
260 265 270
Lys Gly Ser Phe Glu Gly Val Tyr Gly Ala Lys Leu Asn Val Lys Thr
275 280 285
Tyr Asp Ile Thr Asp Lys Asn Asn Ile Lys Leu Val Asp Ser Arg Ser
290 295 300
Ile Asp Leu
305
<210> 200
<211> 441
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 200
Met Tyr Ala His Lys Gln Pro Phe Phe Phe Ser Phe Leu Asn Val Gln
1 5 10 15
Ser Ser Ala Gly Asp Tyr His Tyr Ser Glu Phe Gln Arg Tyr Ser Ala
20 25 30
Gln Tyr Ala Asp His Cys Glu Leu Val Val Asn Asn Gln Leu Asn Lys
35 40 45
Ile Gln Asn Thr Thr Gly Lys Thr Trp Lys Asp Tyr Asn Glu Tyr Arg
50 55 60
Arg Lys Met Ile Leu Ser Val Phe Asp Ile Val Ala Glu Phe Ser Thr
65 70 75 80
Phe Asp Pro Ile Leu Tyr Lys Gly Ala Ile Asn Arg Glu Ile Leu Thr
85 90 95
Arg Lys Ile Tyr Thr Asp Pro Val Asn Phe Thr Pro Gly Ser Ser Ile
100 105 110
Ala Asn Asp Glu Asn Lys Tyr Thr Val Pro Pro Ser Arg Val Asn Gln
115 120 125
Leu Val Ala Ser Thr Leu Phe Thr Asn Val Ala Ser Ala Gln Tyr Ala
130 135 140
Gly Phe Ile Gly Asn Ile Asn Arg Tyr Leu Ser Leu Ser Arg Gly Glu
145 150 155 160
Pro Phe Asp Gly Pro Leu Ile Gly Asn Gln Val Tyr Glu Lys Val Leu
165 170 175
Ala Gly Val Pro Thr Asn Gln Ser Ile Tyr Glu Val Gly Val Glu Gly
180 185 190
Ile Gln Asn Asp Tyr Pro Arg Asn Ile Gly Leu Arg Trp Gly Ser Leu
195 200 205
Thr Ala Phe Gln Asn Phe Tyr Ala Gly Gly Thr Thr Asn Leu Gly Ser
210 215 220
Leu Thr Arg Val Ser Val Pro Pro Lys Asn Asn Asp Pro Ile Asn Ile
225 230 235 240
Thr Asn Phe Thr His Gln Leu Ser Asp Ile Ile Leu Pro Gly Asn Ser
245 250 255
Gly Ser Ser Phe Ala Trp Thr His Val Glu Val Asn Pro Thr Gly Asn
260 265 270
Tyr Leu Ser Thr Asn Gln Ile Asn Leu Ile Ala Gly Thr Lys Ala Ser
275 280 285
Glu Ser Pro Gln Ile Lys Lys Gly Pro Arg Phe Ile Gly Gly Asp Leu
290 295 300
Leu Thr Ala Asp Trp Arg Thr Gly Asn Ile Ile Ser Pro Ser Tyr Gln
305 310 315 320
Leu Ser Pro Lys Phe Pro Gly Thr Arg Thr Ser Phe Arg Val Arg Val
325 330 335
Arg Tyr Gly Ala Asn Ser Ser Gly Ser Met Gln Phe Thr Phe Gly Ala
340 345 350
Thr Thr Ser Ser Gln Leu Ala Tyr Thr Ala Thr Gly Tyr Ser Pro Thr
355 360 365
Asn Thr Lys Tyr Asn Gln Phe Arg Thr Ile Glu Phe Phe Thr Pro Leu
370 375 380
Glu Ile Thr Thr Glu Gly Lys Leu Ile Leu Val Leu Tyr Asn Thr Asn
385 390 395 400
Ser Ala Pro Thr Phe Val Ile Asp Arg Leu Glu Leu Ile Pro Met Ile
405 410 415
Gly Leu Pro Thr Glu Tyr Asn Glu Pro Gln Lys Leu Glu Thr Ala Lys
420 425 430
Lys Ala Val Thr Asp Leu Phe Thr Asn
435 440
<210> 201
<211> 415
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 201
Met Tyr Ala His Lys Gln Pro Phe Phe Phe Ser Phe Leu Asn Val Gln
1 5 10 15
Ser Ser Ala Gly Asp Tyr His Tyr Ser Glu Phe Gln Arg Tyr Ser Ala
20 25 30
Gln Tyr Ala Asp His Cys Glu Leu Val Val Asn Asn Gln Leu Asn Lys
35 40 45
Ile Gln Asn Thr Thr Gly Lys Thr Trp Lys Asp Tyr Asn Glu Tyr Arg
50 55 60
Arg Lys Met Ile Leu Ser Val Phe Asp Ile Val Ala Glu Phe Ser Thr
65 70 75 80
Phe Asp Pro Ile Leu Tyr Lys Gly Ala Ile Asn Arg Glu Ile Leu Thr
85 90 95
Arg Lys Ile Tyr Thr Asp Pro Val Asn Phe Thr Pro Gly Ser Ser Ile
100 105 110
Ala Asn Asp Glu Asn Lys Tyr Thr Val Pro Pro Ser Arg Val Asn Gln
115 120 125
Leu Val Ala Ser Thr Leu Phe Thr Asn Val Ala Ser Ala Gln Tyr Ala
130 135 140
Gly Phe Ile Gly Asn Ile Asn Arg Tyr Leu Ser Leu Ser Arg Gly Glu
145 150 155 160
Pro Phe Asp Gly Pro Leu Ile Gly Asn Gln Val Tyr Glu Lys Val Leu
165 170 175
Ala Gly Val Pro Thr Asn Gln Ser Ile Tyr Glu Val Gly Val Glu Gly
180 185 190
Ile Gln Asn Asp Tyr Pro Arg Asn Ile Gly Leu Arg Trp Gly Ser Leu
195 200 205
Thr Ala Phe Gln Asn Phe Tyr Ala Gly Gly Thr Thr Asn Leu Gly Ser
210 215 220
Leu Thr Arg Val Ser Val Pro Pro Lys Asn Asn Asp Pro Ile Asn Ile
225 230 235 240
Thr Asn Phe Thr His Gln Leu Ser Asp Ile Ile Leu Pro Gly Asn Ser
245 250 255
Gly Ser Ser Phe Ala Trp Thr His Val Glu Val Asn Pro Thr Gly Asn
260 265 270
Tyr Leu Ser Thr Asn Gln Ile Asn Leu Ile Ala Gly Thr Lys Ala Ser
275 280 285
Glu Ser Pro Gln Ile Lys Lys Gly Pro Arg Phe Ile Gly Gly Asp Leu
290 295 300
Leu Thr Ala Asp Trp Arg Thr Gly Asn Ile Ile Ser Pro Ser Tyr Gln
305 310 315 320
Leu Ser Pro Lys Phe Pro Gly Thr Arg Thr Ser Phe Arg Val Arg Val
325 330 335
Arg Tyr Gly Ala Asn Ser Ser Gly Ser Met Gln Phe Thr Phe Gly Ala
340 345 350
Thr Thr Ser Ser Gln Leu Ala Tyr Thr Ala Thr Gly Tyr Ser Pro Thr
355 360 365
Asn Thr Lys Tyr Asn Gln Phe Arg Thr Ile Glu Phe Phe Thr Pro Leu
370 375 380
Glu Ile Thr Thr Glu Gly Lys Leu Ile Leu Val Leu Tyr Asn Thr Asn
385 390 395 400
Ser Ala Pro Thr Phe Val Ile Asp Arg Leu Glu Leu Ile Pro Met
405 410 415
<210> 202
<211> 192
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 202
Met Ala Ala Ser Phe Arg Thr Val Val Glu Val Ala Gln Asp His Leu
1 5 10 15
Ala Gln Ala Arg Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro
20 25 30
Ala Thr Val Asn Phe Asp Phe Glu Ala Ile His Arg Ala Ala Ser Ala
35 40 45
Val Pro Asp Ser Asp Val Val Lys Met Val Arg Gly Trp Gly Leu Gln
50 55 60
Glu His Ala Pro Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val
65 70 75 80
Arg Gln Ala Leu Pro Pro Glu Cys Ala Glu Ala Gly Phe Trp Ala Thr
85 90 95
Val Glu Arg Glu Leu Val Gln Ala Phe Thr Gly Leu Ala Ala Gln Glu
100 105 110
Gly Arg Pro Gly Leu Ser Phe Tyr Glu Glu Ser Gly Asp Arg Thr Arg
115 120 125
Phe Tyr Arg Asp Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Asp
130 135 140
His Met Tyr Ala Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro
145 150 155 160
Lys Ala Glu Thr Leu Ala Leu Asp Leu Thr Asp Val Val Ser Phe Ala
165 170 175
Val Arg Leu Asn Ala Ile Val Val Arg Gln Ala Leu Ala Val Ser Val
180 185 190
<210> 203
<211> 319
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 203
Met Thr Asn Thr Gln Met Asp Asn Lys Tyr Leu Phe Arg Pro Ser Tyr
1 5 10 15
Glu Ala Lys Pro Asp Gly Thr Thr Val Asn Pro Tyr Tyr Ile Ile Ile
20 25 30
Asn Asp Leu Lys Arg Trp Pro Cys Tyr Val Thr Asp Asn Arg Asn Phe
35 40 45
Ile Ser Ser Ser Glu Glu Leu Gln Thr Arg Val Ala Pro Ser Tyr Asp
50 55 60
Pro Thr Gly Lys Tyr Asp Gly Thr Pro Ser Ala Asp Phe Asn Arg Val
65 70 75 80
Tyr Arg Asp Tyr Ala Leu Asp Pro Ala Phe Arg Pro Leu Tyr Ala Ile
85 90 95
Asn Ala Gln Ile Leu Pro Lys Tyr Asn Tyr Trp His Gln Gly Asn Trp
100 105 110
Gln Tyr Asn Glu Leu Gly Arg Val Ser Gln Leu Gly Gln Asp Ile Gln
115 120 125
Asn Phe Ala Asn Leu Ile Cys Val Lys Tyr Ile Asn Glu Leu Asn Gln
130 135 140
Ile Ile Asp Asp Thr Gln Asn Asp Lys Leu Ser Gln Lys Asp Ile Asp
145 150 155 160
Thr Phe Lys Asn Ile Cys Ser Asp Leu Gln Ser Gln Ile Gly Pro Cys
165 170 175
Gln Arg Arg Ser Arg Asn Ile Tyr Asn Glu Tyr Gln Asn Ala Ile Asn
180 185 190
Ile Leu Leu Ser Gln Ser His His Asp Ala Ala Asn Tyr Ala Phe Thr
195 200 205
Ile Val Asp Asn Ala Tyr Ser Thr Ala Lys Ser Gly Asp Gln Asn Ala
210 215 220
Leu Gly Gly Ile Arg Ser Tyr Ala Ser Ser Val Leu Asn Tyr Ala Asn
225 230 235 240
Ala Tyr Asp Gln Gly Val Ile Thr Ala Ser Met Asn Leu Gln Gly Met
245 250 255
Trp Met Phe Phe Gly Asp Asn Leu Gln Glu Leu Ile Ser Asn Leu Lys
260 265 270
Asp Pro Ile Val Asp His Asn Ala Leu Ile Ala Ser Leu Gln Leu Glu
275 280 285
Ile Ala Ile Glu Glu Trp Gln Asn Val Ser Thr Asn Ala Ala Glu Phe
290 295 300
Ile Lys Glu Trp Ala Gly Tyr Gly Ala Ser Leu Gly Tyr Asp Cys
305 310 315
<210> 204
<211> 307
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 204
Met Ser Ile Tyr Asn Tyr Arg Ile Thr Ser Trp Phe Gly Asp Arg Ser
1 5 10 15
Ile Leu Pro Ser Asn Glu Thr Leu Gln Gln Gln Ser Trp Thr Gly Glu
20 25 30
Ala Lys Gln Lys Trp Asn Leu Arg Asp Leu Gly Asp Tyr Ser Phe Glu
35 40 45
Ile Val Asn Gln Ala Asn Gly Lys Val Pro Thr Tyr Gln Trp Ala Gly
50 55 60
Ser Leu Val Asp Asn Val Glu Tyr Leu Lys Leu Asp Tyr Ser Asn Pro
65 70 75 80
Ser Asp Pro Asp Lys Val Phe Gly Ile Ile Asn Arg Ser Phe Leu Phe
85 90 95
Asp Gly Thr Asn Tyr Ala Ile His Ala Glu Asp Leu Val Lys Ile Gln
100 105 110
Lys Leu Pro Asp Val Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr Gly
115 120 125
Gly Val Asp Gln Gln Leu Pro Gln Val Ser Asp Ser Val Val Val Gly
130 135 140
Ala Ser Leu Ile Pro Cys Ile Met Val Asn Asp Asn Gln Ala Ser Asp
145 150 155 160
Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu
165 170 175
Tyr Trp Asp Lys Thr Tyr Ser Ala Val Ile Pro Ala Gly Val Thr His
180 185 190
Lys Tyr Ser Phe Thr Thr Gly Met Ser Ser Val Asp Gln Gln Lys Met
195 200 205
Thr Asp Thr Leu Ser Met Ser Ile Gly Ala Asp Phe Gly Leu Ser Phe
210 215 220
Gly Ala Ala Ser Ala Ser Leu Lys Thr Glu Ile Ser Arg Thr Ile Gln
225 230 235 240
Thr Glu Ile Ser Ser Thr Asn Thr Asn Ala Ser Glu Glu Asn Val Thr
245 250 255
Arg Asp Val Gln Ser Glu Pro Gly Lys Thr Thr Gly Phe Thr Glu Tyr
260 265 270
Gln Leu Ala Thr Arg Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val
275 280 285
Ser Asp Pro Trp Val Val Lys Asn Asn Lys Ile Thr Ile Thr Arg Lys
290 295 300
Asn Ala Gln
305
<210> 205
<211> 295
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 205
Met Asp Gln Ile Asn Thr Ser Glu Asn Asn Leu Gln Arg Glu Val Lys
1 5 10 15
Ala His Thr Ile Leu Asp Trp Lys Asp Pro Ile Phe Asn Ala Thr Glu
20 25 30
Ile Val Gly Lys Asn Leu Leu Val Pro Gly Ile Tyr Leu Phe Thr Asn
35 40 45
Ser Thr Tyr His His Met Gly Tyr Tyr Gly Val Lys Tyr Glu Gln Phe
50 55 60
Ser Val Glu Ala Asp Gly Ser Pro Ser His Thr Asn Ser Thr Ser Ile
65 70 75 80
Phe Val Gly Lys Thr Thr Leu Thr Asn Ile Thr Asp Gln Glu Gln Thr
85 90 95
Leu Ser Thr Asn Ser Phe Ser Lys Met Ile Ser Asn Ser Val Ser Asn
100 105 110
Ser Thr Thr His Gly Phe Lys Phe Gly Thr Lys Ala Ser Ala Lys Phe
115 120 125
Gln Ile Pro Phe Val Gly Glu Thr Gly Ile Glu Leu Ser Ala Glu Tyr
130 135 140
Asn Phe Ser Asp Thr Ser Ser Thr Thr Asn Thr Glu Ser Tyr Thr Tyr
145 150 155 160
Ile Ala Ser Pro Gln Asn Ile Lys Val Pro Ala His Ser Thr Val Glu
165 170 175
Val Leu Val Asn Leu Asn Thr Val Lys Ala Lys Gly Asn Val Lys Leu
180 185 190
Leu Thr Lys Val Ser Gly Arg Asp Gln Gly Val Phe Val Phe Asp Pro
195 200 205
Gln Thr Gly Asp Ile Ser Lys Ser Tyr Tyr Tyr Asp Leu His Phe Asn
210 215 220
Gln Ile Val Glu Lys Ala Ser Lys Phe Glu Lys Leu His Asn Ile Ser
225 230 235 240
Ser Asn Ser Asp Gly Asn Thr Val Asn Ile Ile Gly Ser Gly Lys Tyr
245 250 255
Glu Ala Glu Tyr Gly Thr Glu Phe Ser Val Thr Val Arg Pro Val Asp
260 265 270
Asn Lys Gly Arg Ser Ile Asn Gly Gly Tyr Thr Phe Asn Val Lys Pro
275 280 285
Glu Lys Ile Ile Lys Val Glu
290 295
<210> 206
<211> 315
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 206
Met Pro Pro Gln Val Tyr Ala Glu Ala Ser Ile Ala Gln Glu Lys Ser
1 5 10 15
Ile Gly Ile Val Asp Val Asn Glu Val Leu Lys Lys Val Gly Thr Tyr
20 25 30
Tyr Tyr Lys Asn His Leu Ala Asn Thr Val Ala Asn Pro Glu Ser Asn
35 40 45
Gly Phe Arg Tyr Thr Leu Arg Ala Asn Pro Asp Ser Val Thr Pro Val
50 55 60
Ile Asp Leu Ser Ile Glu Ala Arg Thr Glu Asn Ile His Tyr Asp Ser
65 70 75 80
Thr Ile Pro Ile Phe Ile Gly Glu Asn Thr Phe Pro Asn Asn Leu Asp
85 90 95
Glu Glu Gln Ser Tyr Asn Thr Leu Lys Tyr Ser Lys Thr Tyr Thr Glu
100 105 110
Ser Thr Ser Thr Ser Ile Ser Ser Gly Phe Lys Val Gly Gly Ala Gly
115 120 125
Asp Leu Gly Asn Lys Phe Ile Leu Pro Leu Ile Leu Glu Gly Ala Gly
130 135 140
Lys Ile Asn Leu Glu Tyr Asn Thr Gly Ser Thr Glu Thr Asn Thr Thr
145 150 155 160
Ser Glu Ser Tyr Thr Leu Glu Val Pro Pro Gln Pro Val Lys Val Pro
165 170 175
Pro His Lys Thr Tyr Lys Thr Val Val Glu Phe Ser Gln Arg Thr Tyr
180 185 190
Arg Gly Asp Val Val Phe Thr Gly Arg Thr Glu Ser Thr Met Ala Asn
195 200 205
Arg Ser Ile Ile Asn Ala Thr Gly Ile Tyr Thr Gly Trp Met Gly Met
210 215 220
Gln Asp Ser Lys Lys Phe Thr Tyr Asp Asn Tyr Leu Gly Val His Tyr
225 230 235 240
Ala Gly Leu Thr Ala Ser Gln Gln Gln Glu Ile Lys Asn Ala Gly Val
245 250 255
Gln Ile Thr Thr Ser Gly Tyr Gly Ala Ala Ala Asn Arg Phe Ser Ser
260 265 270
Ile Glu Val Thr Gly Lys Gly Ser Phe Ser Gly Val Ile Gly Ala Glu
275 280 285
Leu Ile Val Thr Thr Tyr Asp Val Thr Asp Ala Lys Asn Ala Arg Leu
290 295 300
Val Asn Gln Arg Thr Val Pro Ile Glu Leu Asp
305 310 315
<210> 207
<211> 653
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 207
Met Lys Arg Asn Lys Asp Cys Asn Asp Gly Ile Gln Lys Ser Asp Met
1 5 10 15
Ser Asp Arg Tyr Pro Tyr Ala Asn Asp Pro Asn Ala Ala Leu Gln Asn
20 25 30
Met Glu Tyr Lys Glu Trp Leu Ser Val Cys Gln Glu Gly Gly Gln Gln
35 40 45
Val Leu His Leu Glu Ser Val Gly Asn Glu Thr Val Asn Ala Val Ser
50 55 60
Ala Gly Val Ile Val Val Gly Thr Met Met Ser Ala Met Ser Ala Pro
65 70 75 80
Leu Ala Ile Pro Gly Gly Ile Leu Ile Ser Phe Gly Thr Leu Ala Ala
85 90 95
Ala Leu Trp Pro Asp Glu Glu Asn Pro Thr Lys Val Trp Ser Asp Phe
100 105 110
Met Lys His Gly Glu Thr Leu Phe Asp Lys Lys Ile Ser Glu Leu Val
115 120 125
Lys Lys Gln Ala Gln Ala His Phe Glu Gly Ile Lys Thr Leu Leu Val
130 135 140
Asp Tyr Glu Lys Ala Phe Asn Asp Trp Lys Glu Asn Lys Asn Arg Leu
145 150 155 160
Thr Ala Glu Lys Val Lys Arg Glu Phe Asn Asp Val Asp Arg Gln Ile
165 170 175
Lys Thr Val Ile Pro His Leu Lys Tyr Glu Gly Tyr Glu Ala Leu Leu
180 185 190
Leu Ser Cys Phe Ala Gln Val Ala Asn Leu His Leu Asn Leu Leu His
195 200 205
Gln Gly Ala Ala Phe Gly Asp Glu Trp Asp Lys Tyr Ser Ser Tyr Leu
210 215 220
Ser Thr Ser Glu Asp Cys Tyr Glu Asp Leu Met Ser Arg Ile Glu Thr
225 230 235 240
Tyr Ile Asn His Cys Thr Lys Thr Tyr Asn Asp Gly Leu Ile Glu Leu
245 250 255
Lys Asn Lys Pro Asn Ile Lys Trp Asn Ile Tyr Asn Thr Tyr Arg Arg
260 265 270
Glu Met Thr Leu Thr Ala Leu Asp Leu Val Ala Leu Phe Pro Asn Tyr
275 280 285
Asp Val Lys Lys Tyr Pro Ile Gly Thr Gln Thr Glu Leu Thr Arg Glu
290 295 300
Val Tyr Thr Val Leu Asn Ser Met Asp Asp Leu Val Asp Phe Glu Ala
305 310 315 320
Ala Glu Lys Ser Leu Ile Gln Asn Pro His Val Phe Arg Trp Leu Ala
325 330 335
Gly Phe Thr Phe Val Thr Lys Asn Ile Ser Ala Ile Asp Leu Lys Tyr
340 345 350
Leu Ser Gly Ile Arg Asn Trp Tyr Tyr Tyr Thr Asn Phe Asp Thr Leu
355 360 365
Lys Ala Asp Lys Ala Asn Ser Glu Asn Ser Gly Gly Gly Ser Gln Lys
370 375 380
Phe Phe Phe Gly Ala Gly Ser Ile Tyr Lys Leu Glu Ile Ile Arg Asn
385 390 395 400
Met Asp Asp His Trp Gly Gly Leu Pro Phe Ala Asn Glu Val Gln Val
405 410 415
Phe Thr Pro Ser Ile Thr Tyr Asp Asn Lys Gln Ser Gln Thr Asn Ser
420 425 430
Val Tyr Asn Lys Lys Gly Asp Ala Ser Ser Gly Ile Pro Ile Met Asp
435 440 445
Leu Leu Tyr Leu Pro Lys Ala Lys Asp Asp Ser Pro Phe Ser Gln Gln
450 455 460
Asp Ile Pro Ser Ala Asn Asn Tyr Ser His Arg Leu Ser Tyr Ile Ala
465 470 475 480
Arg Val Lys Lys Ile Arg Gly Thr Arg Asp Val Tyr Asn Tyr Ala Phe
485 490 495
Ala Trp Thr His Arg Ser Val Asn Arg His Asn Thr Ile Ala Lys Asp
500 505 510
Val Ile Thr Gln Ile Pro Ala Val Lys Ala Leu Glu Pro Asn Lys Asp
515 520 525
Phe Asp Val Ile Lys Gly Pro Gly His Thr Gly Gly Asp Leu Val Lys
530 535 540
Leu Asn Tyr Asn Glu Thr Ser Gly Leu Glu Ile Lys Cys Leu Thr Glu
545 550 555 560
Asn His Pro Lys Ile Asp Tyr Arg Ile Arg Ile Arg Tyr Ala Ala Asn
565 570 575
Glu Asp Thr Arg Val Glu Ile Ile Val Pro Glu Ser Asp Gly Phe Gly
580 585 590
Ser Ile Lys Pro Ala Lys Lys Thr Tyr Ser Asp Asp Asn Tyr Gln Lys
595 600 605
Tyr Asn Ser Phe Gln Tyr Leu Asp Thr Arg Ala Val Val Tyr Gly Glu
610 615 620
Gly Lys Gln Val Ala Leu Ser Ile Lys Asn Leu Glu Pro Asp Thr Phe
625 630 635 640
Ile Leu Ile Asp Lys Ile Glu Phe Ile Pro Trp Phe Gly
645 650
<210> 208
<211> 291
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 208
Met Thr Glu Asn Thr Pro Thr Ser Val Asn Glu Asn Val Lys Val Gly
1 5 10 15
Val Thr Asp Val Gln Ser Glu Leu Lys Lys Ile Gly Glu Tyr His Tyr
20 25 30
Thr Asn Asn Ile Ala Gly Thr Lys Ile Glu Ser Trp Pro Thr Leu Thr
35 40 45
Phe Gly Ser Arg Pro Asp Ser Val Glu Thr Lys Val Asn Phe Ser Ile
50 55 60
Thr Gly Thr Glu Asn Lys Leu Asn Phe Asp Ser Lys Ile Val Glu Tyr
65 70 75 80
Val Gly Glu Asn Ile Phe Glu Asn Thr Thr Glu Gln Ser Gln Lys Phe
85 90 95
Ser Thr Ala Lys Tyr Thr Lys Ser Val Thr Glu Ser Ile Ala Thr Ala
100 105 110
Thr Thr Lys Gly Phe Lys Val Gly Gly Ser Gly Asp Gly Ser Asn Ile
115 120 125
Phe Thr Ile Pro Leu Leu Leu Asn Asn Gly Ile Lys Ile Asn Gly Glu
130 135 140
Phe Asn Ser Ser Thr Thr Glu Thr Lys Thr Lys Ser Glu Thr Arg Ser
145 150 155 160
Ile Glu Ala Ser Ala Gln Ser Ile Glu Val Pro Pro His Lys Lys Tyr
165 170 175
Lys Ala Asp Val Val Leu Glu Gln Arg Asn Phe Trp Gly Asp Val Thr
180 185 190
Phe Thr Gly Val Gly Val Lys Pro Ala Thr Thr Ile Lys Gly Thr Ala
195 200 205
Ser Tyr Trp Ala Pro Asn Gly Met Gly Ala Trp Lys Gln Tyr Thr Phe
210 215 220
Ser Asp Asn Thr Val Asn Tyr Trp Lys Gly Leu Thr Ala Ser Gln Lys
225 230 235 240
Asn Ser Ile Asn Gly Ile Lys Phe Glu Asn Asn Asp Val Lys Ala Glu
245 250 255
Gly Thr Ala Lys Val Glu Gly Thr Phe Gly Ser Met Leu Ile Val Lys
260 265 270
Val Tyr Asp Ile Thr Asp Lys Ser Asn Pro Lys Leu Val Glu Thr Arg
275 280 285
Ser Phe Lys
290
<210> 209
<211> 369
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 209
Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys Asn Lys Asn Ser
1 5 10 15
Arg Lys Tyr Leu Asp Ile Glu Gly Asn Gln Thr Gly Asn Asn Ala Asn
20 25 30
Val Gln Gln Tyr Glu Tyr Leu Ala Asp Ala Leu Ser Glu Arg Phe Phe
35 40 45
Leu His Lys Leu Asp Asp Asn Tyr Tyr Ala Ile Ile Asn Leu Asn Ser
50 55 60
Gly Lys Val Ile Asp Ile Arg Gly Asn Gln Thr Gly Asn Asn Ala Asn
65 70 75 80
Ile Gln Gln Phe Glu Trp Arg Gly Asp Ala Asn Ser Glu Tyr Trp Tyr
85 90 95
Phe Arg Arg Glu Glu Asp Gly Tyr Tyr Val Ile Glu Ser Lys His Ser
100 105 110
Gly Lys Val Ile Asp Ile Glu Gly Asn Gln Thr Gly Asn Asn Ala Asn
115 120 125
Val Gln Gln Phe Gln Phe Leu Lys Asp Ala Pro Ser Glu Arg Phe Ala
130 135 140
Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile Asn Thr Gln Pro
145 150 155 160
Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp Gln Leu Pro Glu
165 170 175
Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val Pro Ala Ile Ala
180 185 190
Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys Gln Ile Lys Glu
195 200 205
Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp Lys Lys Gln Glu
210 215 220
Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Asn Phe Glu Val Thr Thr
225 230 235 240
Gly Ile Arg Val Thr Asp Gln Glu Thr Ala Thr Lys Thr Val Ser Leu
245 250 255
Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly Val Ser Leu Gly
260 265 270
Met Ser Ser Gln Tyr Ser Gln Glu Leu Gln Thr Ser Ile Ser His Thr
275 280 285
Thr Glu Gln Leu Lys Glu Glu Thr Lys Arg His Thr Ile Thr Asn Leu
290 295 300
Phe Ser Glu Arg Met Ala Tyr Ser Arg Tyr Ile Leu Thr Thr Glu Tyr
305 310 315 320
Ser Val Arg Arg Asp Ser Gly Thr Ile Val Asn Ser Ser Trp Thr Met
325 330 335
Thr Asp Lys Thr Arg Thr His Ala Val Thr Phe Pro Lys Ser Thr Gly
340 345 350
Lys Val Leu Asn Glu Asn Ile Lys Gln Ile Ser Lys Ser Glu Ser Leu
355 360 365
Lys
<210> 210
<211> 466
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 210
Met Phe Ala Glu Glu Lys Asp Lys Asn Ala Pro Gln Gln Gln Asn Thr
1 5 10 15
Ile Gln Gln Lys Asp Ala Gly Thr Tyr Tyr Glu Gly Glu Gln Lys Glu
20 25 30
Leu Asn Pro Leu Ala Gln Leu Asp Lys Trp Thr Gln Asp Leu Gly Lys
35 40 45
Thr Ser Gly Ala Gly Asp Tyr Lys Thr Thr Leu Gly Met Val Glu Lys
50 55 60
Leu Leu Pro Thr Ile Tyr Lys Asp Leu Asn Ser Gly Asn Phe Asn Asn
65 70 75 80
Thr Ala Arg Ser Phe Thr Met Leu Ser Thr Ala Leu Ile Pro Tyr Gly
85 90 95
Gly Ala Phe Ile Ser Pro Ile Val Gly Leu Ile Trp Pro Glu Asn Thr
100 105 110
Thr Ala Gln Asp Ala Lys Leu Gln Lys Met Val Glu Gly Leu Thr Ser
115 120 125
Met Met Gly Gln Lys Ile Asn Asp Tyr Asp Leu Ala Lys Leu Lys Gln
130 135 140
Lys Thr Asp Ala Leu Thr Asn Thr Leu Lys Lys Phe Gln Asp Ser Val
145 150 155 160
Asn Asn Lys Pro Gln Ser Glu Ser Tyr Tyr Asp Asn Ile Gln Thr Ala
165 170 175
Asn Val Thr Tyr Ala Asp Tyr Ile Asn Thr Lys Phe Glu Glu Ile Ile
180 185 190
Lys Glu Ser Gln Thr Pro Gly His Gln Ile Pro Glu Leu Pro Ile Phe
195 200 205
Thr Val Ile Ala Thr Ala His Leu Gln Phe Leu His Phe Ile Glu Glu
210 215 220
Asn Ala Val Lys Asn Pro Arg Ile Lys Met Asp Pro Glu His Leu Asn
225 230 235 240
Arg Tyr Phe Pro Lys Phe Lys Asp Thr Ala Arg Lys Tyr Arg Lys Tyr
245 250 255
Ile Asn Asp Val Asn His Asp Ala Gln Gln Trp Leu Tyr Asp Phe Thr
260 265 270
Gly Gly Gly His Ile Lys Ala Asn Asn Pro Ala His Ile Glu Ala Met
275 280 285
Asn Lys Ile Ala Asp Leu Thr Lys Gln Thr Ser Asp Asn Glu Ala Phe
290 295 300
Leu Leu Ala Ser Asn Pro Pro Ser Val Gly Asp Lys Thr Gly Leu Val
305 310 315 320
Lys Glu Asp Gly Asp Lys Trp His Tyr Tyr Ser Pro Arg Asp Gly Phe
325 330 335
Glu Asn Ser Gln His Gln Lys Phe Asn Lys Gly Ser Leu Val Thr Gly
340 345 350
Pro Leu Asp Ile Asp Gly Arg Thr Tyr Leu Phe Ser Phe Glu Glu Gly
355 360 365
Met Lys Asn Ser Ala Gly Lys Lys Phe Asn Lys Gly Asp Met Phe Thr
370 375 380
Gly Trp Tyr Asn Asp Ala Gly Val Trp Ser Tyr Tyr Lys Pro Glu Ser
385 390 395 400
Gly Val Met Ala Thr Asp Trp Gln Asn Ile Ser Gly Lys Trp Tyr Tyr
405 410 415
Phe Ala Thr Ala Tyr Gln Glu Phe Asn Gly Gly Ala Phe Ile Ser Lys
420 425 430
Gly Asp Met Ile Lys Gly Lys Thr Val Glu Ile Ser Gly Lys Leu Tyr
435 440 445
Thr Phe Asp Glu Asn Gly Ala Cys Leu Asn Pro Asp Gly Lys Pro Ile
450 455 460
Ala Lys
465
<210> 211
<211> 1876
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 211
Met Ala Ser Leu Ala Asp Leu Tyr Pro Ser Tyr Tyr Asn Val Leu Ala
1 5 10 15
Glu Pro Pro Phe Ile Asn Gly Asp Asn Asp Ile Ile Ile Thr Ala Lys
20 25 30
Ser Asp Cys Lys Glu Asp Ser Ala Ser Glu Ala Ala Gly Asn Ser Asn
35 40 45
Lys Asn Asn Tyr Trp Ala Gly Ala Gln Ser Thr Trp Lys Cys Tyr Trp
50 55 60
Gln Ser Gly Tyr Gln Asp Ala Tyr Thr Gly Val Ile Ser Ala Ala Phe
65 70 75 80
Ser Asp Asp Asn Phe Asn Tyr Val Asp Phe Thr Lys Lys Val Ile Gly
85 90 95
Leu Asn Gly Phe Phe Ile Ser Trp Leu Gly Gly Glu Ile Ser Glu Glu
100 105 110
Leu Arg Ser Thr Leu Asn Gly Ala Glu Lys Ala Leu Glu Val Ala Gly
115 120 125
Met Val Thr Leu Pro Ile Val Val Pro Ile Ala Gln Ala Leu Val Met
130 135 140
Ala Ile Phe Pro Ile Ser Val Thr Lys Lys Lys Arg Glu Asp Gln Ala
145 150 155 160
Lys Thr Leu Trp Asp Leu Leu Ala Lys Arg Val Asp Glu Glu Ile Lys
165 170 175
Asp Leu Ile Lys Asp Tyr Asp Ala Asn Ala Leu Asn Gln Phe Ile Ala
180 185 190
Gly Phe Gln Thr Val Leu Lys Asp Leu Ser Asp Ala Val Gln Ile Ala
195 200 205
Ile Cys Gln Pro Ile Asp Leu Asn Pro Asp Ile Pro Ser Lys Val Asn
210 215 220
Pro Asn Cys Ile Pro Tyr Thr Asp Ile Glu Leu Val Asn Asn Val Lys
225 230 235 240
Gln Lys Leu Ser Thr Val Asp Ser Ala Phe Thr Thr Leu Ile Pro Gln
245 250 255
Phe Gln Lys Ser Asp Tyr Ala Ser Ala Glu Leu Pro Gln Phe Cys Leu
260 265 270
Ala Ala Thr Leu His Leu Ser Thr Leu His Ser Ala Ile Ile Met Ser
275 280 285
Glu Pro Trp Glu Leu Asn Pro Asn Ile Arg Lys Ser Tyr Ile Arg Lys
290 295 300
Leu Arg Arg Leu Ile Lys Ser Tyr Ser Leu Tyr Val Thr Gln Val Val
305 310 315 320
Thr Lys Ala Ser Gln Asp Tyr Leu Lys Gln Asn Ser Ala Gly Asn Leu
325 330 335
Thr Lys Lys Gly Phe Asn Glu Tyr Asn Gln Leu Leu Ser Phe Tyr Val
340 345 350
Thr Glu Cys Leu Asp Tyr Val Ala Met Trp Arg Phe Leu Asp Pro Ile
355 360 365
Asp Tyr Pro Leu Pro Thr Tyr Ala Glu Leu Thr Arg Val Ile Val Gly
370 375 380
Asn Ile Val Gly Pro Tyr Glu Glu Asp Asn Thr Val Gly Gly Asn Ala
385 390 395 400
Thr Pro Ile Ser Asp Asp Leu Arg Pro Lys Ile Gln Ile Phe Asp Pro
405 410 415
Thr Ser Gln Asp Phe Lys Glu Leu Ser Asn Ser Arg Met Tyr Asp Leu
420 425 430
Leu Gly Leu Thr Tyr Asn Asn Leu Gln Met Lys Asn Val Thr Met His
435 440 445
Ser Gln Gly Asp Ser Arg Asn Val Val Val Ala Gly Glu His Leu Glu
450 455 460
Tyr Met Tyr Tyr Gln Asp Ser Leu Thr Lys Asp Phe Gly Asn Thr Thr
465 470 475 480
Gly His Ser Val Phe Ser Asp Val Ser Leu Asn Pro Ala Thr Tyr Leu
485 490 495
Asn Met Glu Ser Ala Tyr Asn Asn Val Asn Asn Val Asp Gln Thr Glu
500 505 510
Leu His Ile Cys Lys Met Asn Gly Asp Ile Asp Ile Val Gly Ala Val
515 520 525
Asp Phe Glu Thr Cys Asn Ala His Gly Gly Lys Gly Leu Gly Thr Arg
530 535 540
Cys Thr Ser Asn Asn Lys Pro Leu Gly Ile Asp Lys Ile Gln Met Ile
545 550 555 560
Tyr Pro Val Thr Val Glu Ser Ile Ala Gly Asn Gln Ala Lys Met Gly
565 570 575
Phe Val Ala Thr His Val His Asp Ile Val Thr Pro Glu Asn Ile Ile
580 585 590
Gly Gln Lys Met Gln Asp Asn Asp Gly Asn Asp Thr Asp Gln Pro Leu
595 600 605
Leu Gln Ile Arg Thr Ile Ser Val Glu Lys Gly Lys Leu Ser Gly Asn
610 615 620
Ala Arg Pro Ile Lys Glu Tyr Val Thr Gly Ser Asn Pro Val Leu Ile
625 630 635 640
Pro Pro Gly Gly Ser Ile Asp Leu Thr Cys Thr Asn Leu Thr Ala Gln
645 650 655
Lys Tyr Phe Ile Arg Phe Cys Leu Ser Thr Met Ser Asp Ala Leu Ser
660 665 670
Leu Gln Trp Gln Leu Asn Asn Gly Gln Thr Gln Val Val Ser Ile Pro
675 680 685
Thr Ala Thr Ser Asn Phe Ile Ser Asn Asp Gln Lys Gly Ile Ser Gly
690 695 700
Leu Ser Leu Asn Phe Gly Pro Tyr Thr Ala Asp Asn Leu Tyr Ala Val
705 710 715 720
Phe Pro Glu Thr Thr Phe Asp Asp Asn Gly Leu Pro Ile Leu Pro Asp
725 730 735
Glu Ser His Ala Val Glu Ile Gly Asp Gly Glu Phe Thr Val Thr Ile
740 745 750
Thr Asn Pro Ala Pro Ser Val Ser Asn Asp Ser Asn Asp Ile Thr Val
755 760 765
Asp Arg Ile Glu Phe Ile Pro Tyr Ile Glu Gln Pro Ala Pro Glu Leu
770 775 780
Gln Lys Tyr Leu Met Asp Thr Leu Ser Asn Ile Gln Pro Phe Thr Val
785 790 795 800
Gly Leu Asn Gln Pro Thr Ile Ile Trp Ser Ser Thr Asp Arg Val Ala
805 810 815
Thr Glu Leu Asp Met Arg Tyr Glu Gly Tyr His Pro Ser Phe Ile Asn
820 825 830
Ser Asn Pro Arg Leu Asp Phe Tyr Tyr Asn Asn Val Leu Gln Thr Ala
835 840 845
Ile Arg Ser Glu Thr Pro Asp Ile Tyr Gly Tyr Tyr Asn Tyr Thr Ile
850 855 860
Glu Gly Asn Asp Thr Asp Lys Thr Gly Gly Phe Asp Arg Val Glu Leu
865 870 875 880
Ile Ala Asn Thr Thr Asn Leu Glu Thr Ile Thr Leu Ser Gly Lys Val
885 890 895
Tyr Gly Pro Asn Gln Gln Tyr Lys Pro Thr Pro Pro Pro Thr Pro Pro
900 905 910
Thr Pro Pro Thr Pro Ala Ala Thr Ile Val Asp Ser Val Pro Phe Val
915 920 925
Val Gly Lys Gly Ala Asn Lys Thr Ile Trp Ser Ser Thr Ser Ile Val
930 935 940
Ala Asn Gln Ala Asn Leu Lys Leu Val Ala Tyr Gln Pro Asp Phe Ile
945 950 955 960
Thr Tyr Asp Tyr Gln Phe Ala Phe Tyr Tyr Asn Gly Val Leu Gln Asn
965 970 975
Ser Ile Gln Thr Arg Asp Pro Lys Gly Phe Asp Met Tyr Ala Val Ser
980 985 990
Ser Gly Phe Asn Ser Ile Ala Leu Ile Ser Lys Ala Pro Asn Phe Glu
995 1000 1005
Thr Val Arg Leu Ser Gly Asn Ile Tyr Thr Pro Gln Thr Pro Pro
1010 1015 1020
Pro Pro Pro Pro Pro Thr Pro Leu Pro Ala Ala Thr Ile Val Asp
1025 1030 1035
Ser Val Pro Phe Val Val Gly Lys Gly Ala Asp Thr Thr Ile Trp
1040 1045 1050
Arg Ser Thr Ser Ile Val Ala Asn Gln Ala Asn Leu Lys Leu Val
1055 1060 1065
Ala Tyr Gln Pro Asp Phe Leu Thr Ser Glu His Gln Phe Ala Phe
1070 1075 1080
Tyr Tyr Asn Asp Val Leu Gln Lys Val Thr Leu Thr Gly Gln Asp
1085 1090 1095
Ser Lys Gly Phe Asp Ile Tyr Ser Val Ser Ser Gly Phe Asn Ser
1100 1105 1110
Ile Ala Leu Ile Ser Gln Val Pro Asn Phe Glu Thr Val Arg Leu
1115 1120 1125
Ser Gly Asp Ile Tyr Ala Pro Gln Ile Thr Pro Lys Pro Pro Thr
1130 1135 1140
Leu Pro Glu Ser Val Gly Gly Phe Ser Thr Pro Lys Ser Leu Asp
1145 1150 1155
Ile Pro Val Asn Thr Ser Ser Glu Val Trp Arg Asp Tyr Thr Val
1160 1165 1170
Tyr Gly Ile Gln Ala Lys Ile Asn Thr Arg Phe Leu Asp Ser Pro
1175 1180 1185
Asp Arg Asn Ile Thr Tyr Trp Tyr Gln Phe Tyr Leu Thr Ser Gly
1190 1195 1200
Glu Gly Gln Asn Asp Thr Leu Val His Glu Glu Lys Ala Lys Ser
1205 1210 1215
Gly Asp Tyr Glu Tyr Ile Val Asn His Tyr Phe Asn Lys Ile Val
1220 1225 1230
Leu His Val Thr Gly Phe Tyr Pro Val Ser Glu Pro Ser Thr Ser
1235 1240 1245
Ser Val Thr Ile Glu Ser Cys Ala Ile Tyr Gly Tyr Glu Arg Gln
1250 1255 1260
Lys Ile Asp Pro Ser Val Asn Lys Thr Ile Glu Pro Met Asp Thr
1265 1270 1275
Phe Gly Arg Lys Ser Ile Glu Ile Trp Thr Pro Thr Lys Pro Thr
1280 1285 1290
Ala Lys Gly Phe Glu Ala Ile Leu Gly Val Asp Val Ile Thr Met
1295 1300 1305
Gly Ala Trp Ser Ser Asp Thr Val Tyr Gln Phe Tyr Leu Asn Gly
1310 1315 1320
Gln Leu Val Thr Glu Gln Pro Val Asp Phe Thr Asn Leu Met Lys
1325 1330 1335
Asn Val Tyr Glu Val Pro Gln Gly Phe Asn Ser Ile Ser Ile Phe
1340 1345 1350
Asp Pro Asn Ala Tyr Asp Pro Asp Asp Pro Asp Asp Asn Gly Asn
1355 1360 1365
Tyr Pro Leu Thr Gly Asp Glu Tyr Asn Ile Thr Ile Ile Gly Thr
1370 1375 1380
Ile Phe Gly Asp Ala Ser Ser Ser Asn Leu Pro Pro Glu Asn Pro
1385 1390 1395
Val Asp Gly Thr Met Phe Thr Thr Arg Glu Ala Phe Met Thr Ile
1400 1405 1410
Ala Arg Leu Val Tyr Ala Leu Phe Thr Thr Ser Ser Gln Thr Thr
1415 1420 1425
Leu Asn Leu Thr Ile Thr Asp Tyr Trp Ile His Gln Leu Val Met
1430 1435 1440
Lys Val Asn Lys Leu Ser Asn Glu Lys Phe Gly Ile Glu Lys Lys
1445 1450 1455
Glu Leu Leu Lys Leu Leu Ser Lys Ala Lys Gln Ile Leu Lys Gly
1460 1465 1470
Lys Asn Phe Leu Arg Asn Gly Ser Phe Asp Thr Leu Asp Asn Trp
1475 1480 1485
Gln Val Gly Arg Glu Val Ala Ala Ile Tyr Gly His Glu Leu Phe
1490 1495 1500
Gln Gly Asn Tyr Leu Phe Leu Pro Pro Pro Thr Leu Ala Pro Ser
1505 1510 1515
Tyr Ala Tyr Gln Arg Ile Pro Glu Ser Arg Leu Lys Lys Asn Thr
1520 1525 1530
Arg Tyr Lys Ile Ser Gly Phe Ile Ala Ser Ser Leu Glu Leu Glu
1535 1540 1545
Leu Ile Val Ser Arg Tyr Gly Lys Glu Ile Val Lys Thr Leu Asn
1550 1555 1560
Val Pro Tyr Gly Lys Ala Leu Pro Val Ser Pro Asp Ala His Pro
1565 1570 1575
Asn Cys Cys Asn His Asn Leu Cys Gly Cys Pro Ser Cys Ile Gly
1580 1585 1590
Asn Gly Thr Asn Lys His Phe Phe Asn Tyr Cys Ile Asp Val Gly
1595 1600 1605
Thr Val Gln Thr Ile Thr Asp Leu Gly Ile Glu Phe Gly Val Arg
1610 1615 1620
Ile Lys Lys Gly Asn Gly Tyr Ala Lys Leu Gly Tyr Leu Glu Ile
1625 1630 1635
Gln Glu Glu Arg Pro Leu Thr Pro Gln Glu Val Gln Asn Val Gln
1640 1645 1650
Gln Lys Glu Leu Lys Trp Lys Glu Thr Phe Leu Gln Glu Lys Gln
1655 1660 1665
Gln Ile Thr Asn Leu Leu Asn Pro Ile Ile Thr Lys Ile Asn Ala
1670 1675 1680
Phe Phe Lys Asn Lys Asp Trp Asn Ser Asp Ile Leu Pro His Val
1685 1690 1695
Thr Tyr Gln Asp Leu Ser Thr Ile Ile Ile Pro Lys Leu Pro Lys
1700 1705 1710
Met Thr His Trp Phe Met Thr Asp Arg Val Gly Glu His Tyr Glu
1715 1720 1725
Ile Met Gln Lys Leu Gln Gln Ala Leu Gln Arg Val Arg Thr His
1730 1735 1740
Leu Asp Ala Glu Asn Leu Ile His Asn Gly Asp Phe Thr Asp Gly
1745 1750 1755
Leu Gln Asn Trp Glu Ile Gln Gly Asp Val Ser Leu Phe Ala Pro
1760 1765 1770
Gly Ile Leu Gln Leu Ser Asp Trp Asp Ala Ser Val Ser Gln Thr
1775 1780 1785
Ile Glu Ile Leu Asn Phe Thr Ala Gly Gln Gly Tyr Thr Leu Leu
1790 1795 1800
Val Asn Ser Lys Gly Ala Gly Ser Ile Thr Ile Glu His Gly Gln
1805 1810 1815
Val Val Glu Thr Leu His Met Met Ser Pro Asn Phe Met Thr Lys
1820 1825 1830
Thr Leu Thr Phe Tyr Pro Val Ser Pro Thr Leu Glu Ile Gln Ile
1835 1840 1845
Leu Ser Asp Gly Ala Glu Leu Leu Val Asp Ser Val Thr Ile Leu
1850 1855 1860
Gln Asn Pro Ile Glu Asn Ser Asp Ala Thr Ser Cys Glu
1865 1870 1875
<210> 212
<211> 776
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 212
Met Ala Ser Leu Ala Asp Leu Tyr Pro Ser Tyr Tyr Asn Val Leu Ala
1 5 10 15
Glu Pro Pro Phe Ile Asn Gly Asp Asn Asp Ile Ile Ile Thr Ala Lys
20 25 30
Ser Asp Cys Lys Glu Asp Ser Ala Ser Glu Ala Ala Gly Asn Ser Asn
35 40 45
Lys Asn Asn Tyr Trp Ala Gly Ala Gln Ser Thr Trp Lys Cys Tyr Trp
50 55 60
Gln Ser Gly Tyr Gln Asp Ala Tyr Thr Gly Val Ile Ser Ala Ala Phe
65 70 75 80
Ser Asp Asp Asn Phe Asn Tyr Val Asp Phe Thr Lys Lys Val Ile Gly
85 90 95
Leu Asn Gly Phe Phe Ile Ser Trp Leu Gly Gly Glu Ile Ser Glu Glu
100 105 110
Leu Arg Ser Thr Leu Asn Gly Ala Glu Lys Ala Leu Glu Val Ala Gly
115 120 125
Met Val Thr Leu Pro Ile Val Val Pro Ile Ala Gln Ala Leu Val Met
130 135 140
Ala Ile Phe Pro Ile Ser Val Thr Lys Lys Lys Arg Glu Asp Gln Ala
145 150 155 160
Lys Thr Leu Trp Asp Leu Leu Ala Lys Arg Val Asp Glu Glu Ile Lys
165 170 175
Asp Leu Ile Lys Asp Tyr Asp Ala Asn Ala Leu Asn Gln Phe Ile Ala
180 185 190
Gly Phe Gln Thr Val Leu Lys Asp Leu Ser Asp Ala Val Gln Ile Ala
195 200 205
Ile Cys Gln Pro Ile Asp Leu Asn Pro Asp Ile Pro Ser Lys Val Asn
210 215 220
Pro Asn Cys Ile Pro Tyr Thr Asp Ile Glu Leu Val Asn Asn Val Lys
225 230 235 240
Gln Lys Leu Ser Thr Val Asp Ser Ala Phe Thr Thr Leu Ile Pro Gln
245 250 255
Phe Gln Lys Ser Asp Tyr Ala Ser Ala Glu Leu Pro Gln Phe Cys Leu
260 265 270
Ala Ala Thr Leu His Leu Ser Thr Leu His Ser Ala Ile Ile Met Ser
275 280 285
Glu Pro Trp Glu Leu Asn Pro Asn Ile Arg Lys Ser Tyr Ile Arg Lys
290 295 300
Leu Arg Arg Leu Ile Lys Ser Tyr Ser Leu Tyr Val Thr Gln Val Val
305 310 315 320
Thr Lys Ala Ser Gln Asp Tyr Leu Lys Gln Asn Ser Ala Gly Asn Leu
325 330 335
Thr Lys Lys Gly Phe Asn Glu Tyr Asn Gln Leu Leu Ser Phe Tyr Val
340 345 350
Thr Glu Cys Leu Asp Tyr Val Ala Met Trp Arg Phe Leu Asp Pro Ile
355 360 365
Asp Tyr Pro Leu Pro Thr Tyr Ala Glu Leu Thr Arg Val Ile Val Gly
370 375 380
Asn Ile Val Gly Pro Tyr Glu Glu Asp Asn Thr Val Gly Gly Asn Ala
385 390 395 400
Thr Pro Ile Ser Asp Asp Leu Arg Pro Lys Ile Gln Ile Phe Asp Pro
405 410 415
Thr Ser Gln Asp Phe Lys Glu Leu Ser Asn Ser Arg Met Tyr Asp Leu
420 425 430
Leu Gly Leu Thr Tyr Asn Asn Leu Gln Met Lys Asn Val Thr Met His
435 440 445
Ser Gln Gly Asp Ser Arg Asn Val Val Val Ala Gly Glu His Leu Glu
450 455 460
Tyr Met Tyr Tyr Gln Asp Ser Leu Thr Lys Asp Phe Gly Asn Thr Thr
465 470 475 480
Gly His Ser Val Phe Ser Asp Val Ser Leu Asn Pro Ala Thr Tyr Leu
485 490 495
Asn Met Glu Ser Ala Tyr Asn Asn Val Asn Asn Val Asp Gln Thr Glu
500 505 510
Leu His Ile Cys Lys Met Asn Gly Asp Ile Asp Ile Val Gly Ala Val
515 520 525
Asp Phe Glu Thr Cys Asn Ala His Gly Gly Lys Gly Leu Gly Thr Arg
530 535 540
Cys Thr Ser Asn Asn Lys Pro Leu Gly Ile Asp Lys Ile Gln Met Ile
545 550 555 560
Tyr Pro Val Thr Val Glu Ser Ile Ala Gly Asn Gln Ala Lys Met Gly
565 570 575
Phe Val Ala Thr His Val His Asp Ile Val Thr Pro Glu Asn Ile Ile
580 585 590
Gly Gln Lys Met Gln Asp Asn Asp Gly Asn Asp Thr Asp Gln Pro Leu
595 600 605
Leu Gln Ile Arg Thr Ile Ser Val Glu Lys Gly Lys Leu Ser Gly Asn
610 615 620
Ala Arg Pro Ile Lys Glu Tyr Val Thr Gly Ser Asn Pro Val Leu Ile
625 630 635 640
Pro Pro Gly Gly Ser Ile Asp Leu Thr Cys Thr Asn Leu Thr Ala Gln
645 650 655
Lys Tyr Phe Ile Arg Phe Cys Leu Ser Thr Met Ser Asp Ala Leu Ser
660 665 670
Leu Gln Trp Gln Leu Asn Asn Gly Gln Thr Gln Val Val Ser Ile Pro
675 680 685
Thr Ala Thr Ser Asn Phe Ile Ser Asn Asp Gln Lys Gly Ile Ser Gly
690 695 700
Leu Ser Leu Asn Phe Gly Pro Tyr Thr Ala Asp Asn Leu Tyr Ala Val
705 710 715 720
Phe Pro Glu Thr Thr Phe Asp Asp Asn Gly Leu Pro Ile Leu Pro Asp
725 730 735
Glu Ser His Ala Val Glu Ile Gly Asp Gly Glu Phe Thr Val Thr Ile
740 745 750
Thr Asn Pro Ala Pro Ser Val Ser Asn Asp Ser Asn Asp Ile Thr Val
755 760 765
Asp Arg Ile Glu Phe Ile Pro Tyr
770 775
<210> 213
<211> 609
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 213
Met Lys Tyr Lys Glu Arg Lys Asp Ala Lys Arg Lys Tyr Lys Gln Ala
1 5 10 15
Leu Leu Thr Thr Val Ala Thr Met Thr Leu Gly Val Asn Thr Leu Gly
20 25 30
Asn Thr Ser Ser Thr Phe Ala Ala Glu Glu Asp Asn Ile Ser Val Ile
35 40 45
Ser Thr His Thr Ile Asn Asn Val Gly Thr Gln Thr Asp Ile Ser Glu
50 55 60
Glu Ala Lys Lys Lys Thr Phe Phe Ser Lys Val Ser Ala Asn Leu Thr
65 70 75 80
Thr Ala Thr Gly Lys Lys Ile Val Val Ala Gly Glu Lys Glu Leu Leu
85 90 95
Asn Met Ile Lys Thr Gln Lys Val Asp Phe Asn Asn Ala Ser Arg Ala
100 105 110
Leu Val Gly Ala Ile Ile Asp Asn Ala Val Pro Gly Gly Met Phe Leu
115 120 125
Ser Pro Leu Ile Asn Thr Ile Trp Pro Glu Asn Gly Asn Gly Glu Ile
130 135 140
Gln Gln Leu Arg Glu Glu Leu Met Lys Val Met Asp Glu Lys Ile Thr
145 150 155 160
Thr Ser His Met Asn Ser Leu Lys Glu Glu Phe Arg Ala Leu Asn Thr
165 170 175
Ile Gln Asn Lys Phe Glu Lys Ala Val Asn Glu Lys Asn Ser Gln Asn
180 185 190
Val Asp Ala Ser Thr Lys Arg Thr Arg Ser Gly Trp Ala Ser Gly Ile
195 200 205
Glu Gln Ala Tyr Met Arg Leu Leu Ala Leu Thr Ser Ser Asn Asp Phe
210 215 220
Lys Val Ala Asp Leu Pro Leu Tyr Thr Gln Ile Ala Thr Ala His Ile
225 230 235 240
Ala Phe Leu Thr Ser Leu Asp Thr Pro Glu Met Ala Ala Lys Val Asp
245 250 255
Ile Thr Thr Thr Gly Leu Lys Glu Phe Tyr Asn Lys Gly Glu Leu Ser
260 265 270
Lys Leu Ala Asn Glu Tyr Met Arg His Ile Thr Glu Thr Tyr Asp Tyr
275 280 285
Ala Lys Arg Glu Tyr Ile Asn Lys Ile Glu Gln Val Gly Val Ala Phe
290 295 300
Asp Pro Ser Asn Glu Ala Asn Gln Gln Ala Asp Leu Asn Asn Arg Leu
305 310 315 320
His Ser Ala Glu Lys Lys Leu Lys Asp Arg Glu Gly Val Leu Ser Thr
325 330 335
Asn Pro Ala Met Ile Trp Asp Asn Asp Pro Lys Tyr Lys Lys Asn Lys
340 345 350
Thr Glu Val Thr Tyr Leu Lys Asp Ala Lys Asp Ala Tyr Pro Thr Leu
355 360 365
Leu Lys Leu Tyr Lys Leu Thr Val Ser Asp Pro Asn Met Ala Glu Leu
370 375 380
Ser Lys Gly Thr Trp Met Glu Lys Ser Gly Lys Trp His Phe Thr Asn
385 390 395 400
Lys Asn Gly His Asp Met Leu Gly Trp Val Gln Asp Asn Gly Lys Arg
405 410 415
Tyr Tyr Ile Ser Pro Glu Asn Gly Thr Lys Asn Ser Ala Gly Asp Thr
420 425 430
Phe Asn Lys Gly Glu Met Val Thr Gly Trp Lys Glu Val Asp Gly His
435 440 445
Arg Tyr Tyr Phe Ser Pro Lys Lys Thr Asp Thr Gly Asn Asn His Lys
450 455 460
Pro Ile Ala Glu Gly Glu Met Val Thr Asp Trp Lys Glu Val Asp Gly
465 470 475 480
His Arg Tyr Tyr Phe Ser Pro Glu Asn Gly Thr Arg Gly Tyr Glu Arg
485 490 495
Pro Ala Asp Asn Glu Lys Glu Ser Pro Phe Lys Glu Phe Thr Lys Gly
500 505 510
Glu Met Met Thr Gly Phe Ile Gln Arg Gln Ser Asp Tyr Asn Trp Phe
515 520 525
Phe Leu Ser Pro Glu Lys Asn Thr Gln Asn Ser Asp Gly Thr Thr Phe
530 535 540
Val Glu Gly Glu Met Met Thr Ala Trp Tyr Ser Asn Pro Lys Asp Asp
545 550 555 560
Lys His Trp Tyr Tyr Phe Ser Thr Ser Asn Gln Pro Asp Asp Ser Asn
565 570 575
Ser Gln Arg Leu Glu Ser Gly Gln Met Ala Lys Tyr Leu Asn Ile Gly
580 585 590
Gly Tyr Asn Phe Asn Arg Tyr Gly Val Cys Thr Asn Pro Asn Tyr Lys
595 600 605
Lys
<210> 214
<211> 571
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 214
Met Ala Glu Glu Asp Asn Ile Ser Val Ile Ser Thr His Thr Ile Asn
1 5 10 15
Asn Val Gly Thr Gln Thr Asp Ile Ser Glu Glu Ala Lys Lys Lys Thr
20 25 30
Phe Phe Ser Lys Val Ser Ala Asn Leu Thr Thr Ala Thr Gly Lys Lys
35 40 45
Ile Val Val Ala Gly Glu Lys Glu Leu Leu Asn Met Ile Lys Thr Gln
50 55 60
Lys Val Asp Phe Asn Asn Ala Ser Arg Ala Leu Val Gly Ala Ile Ile
65 70 75 80
Asp Asn Ala Val Pro Gly Gly Met Phe Leu Ser Pro Leu Ile Asn Thr
85 90 95
Ile Trp Pro Glu Asn Gly Asn Gly Glu Ile Gln Gln Leu Arg Glu Glu
100 105 110
Leu Met Lys Val Met Asp Glu Lys Ile Thr Thr Ser His Met Asn Ser
115 120 125
Leu Lys Glu Glu Phe Arg Ala Leu Asn Thr Ile Gln Asn Lys Phe Glu
130 135 140
Lys Ala Val Asn Glu Lys Asn Ser Gln Asn Val Asp Ala Ser Thr Lys
145 150 155 160
Arg Thr Arg Ser Gly Trp Ala Ser Gly Ile Glu Gln Ala Tyr Met Arg
165 170 175
Leu Leu Ala Leu Thr Ser Ser Asn Asp Phe Lys Val Ala Asp Leu Pro
180 185 190
Leu Tyr Thr Gln Ile Ala Thr Ala His Ile Ala Phe Leu Thr Ser Leu
195 200 205
Asp Thr Pro Glu Met Ala Ala Lys Val Asp Ile Thr Thr Thr Gly Leu
210 215 220
Lys Glu Phe Tyr Asn Lys Gly Glu Leu Ser Lys Leu Ala Asn Glu Tyr
225 230 235 240
Met Arg His Ile Thr Glu Thr Tyr Asp Tyr Ala Lys Arg Glu Tyr Ile
245 250 255
Asn Lys Ile Glu Gln Val Gly Val Ala Phe Asp Pro Ser Asn Glu Ala
260 265 270
Asn Gln Gln Ala Asp Leu Asn Asn Arg Leu His Ser Ala Glu Lys Lys
275 280 285
Leu Lys Asp Arg Glu Gly Val Leu Ser Thr Asn Pro Ala Met Ile Trp
290 295 300
Asp Asn Asp Pro Lys Tyr Lys Lys Asn Lys Thr Glu Val Thr Tyr Leu
305 310 315 320
Lys Asp Ala Lys Asp Ala Tyr Pro Thr Leu Leu Lys Leu Tyr Lys Leu
325 330 335
Thr Val Ser Asp Pro Asn Met Ala Glu Leu Ser Lys Gly Thr Trp Met
340 345 350
Glu Lys Ser Gly Lys Trp His Phe Thr Asn Lys Asn Gly His Asp Met
355 360 365
Leu Gly Trp Val Gln Asp Asn Gly Lys Arg Tyr Tyr Ile Ser Pro Glu
370 375 380
Asn Gly Thr Lys Asn Ser Ala Gly Asp Thr Phe Asn Lys Gly Glu Met
385 390 395 400
Val Thr Gly Trp Lys Glu Val Asp Gly His Arg Tyr Tyr Phe Ser Pro
405 410 415
Lys Lys Thr Asp Thr Gly Asn Asn His Lys Pro Ile Ala Glu Gly Glu
420 425 430
Met Val Thr Asp Trp Lys Glu Val Asp Gly His Arg Tyr Tyr Phe Ser
435 440 445
Pro Glu Asn Gly Thr Arg Gly Tyr Glu Arg Pro Ala Asp Asn Glu Lys
450 455 460
Glu Ser Pro Phe Lys Glu Phe Thr Lys Gly Glu Met Met Thr Gly Phe
465 470 475 480
Ile Gln Arg Gln Ser Asp Tyr Asn Trp Phe Phe Leu Ser Pro Glu Lys
485 490 495
Asn Thr Gln Asn Ser Asp Gly Thr Thr Phe Val Glu Gly Glu Met Met
500 505 510
Thr Ala Trp Tyr Ser Asn Pro Lys Asp Asp Lys His Trp Tyr Tyr Phe
515 520 525
Ser Thr Ser Asn Gln Pro Asp Asp Ser Asn Ser Gln Arg Leu Glu Ser
530 535 540
Gly Gln Met Ala Lys Tyr Leu Asn Ile Gly Gly Tyr Asn Phe Asn Arg
545 550 555 560
Tyr Gly Val Cys Thr Asn Pro Asn Tyr Lys Lys
565 570
<210> 215
<211> 635
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 215
Met Asn Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Gly His
1 5 10 15
Asn Val Met Ala Gln Asp Pro Phe Asn Phe Gln His Lys Ser Leu Asp
20 25 30
Thr Ile Gln Lys Glu Trp Thr Glu Trp Lys Arg Asp Asp His Ser Leu
35 40 45
Tyr Val Ala Pro Val Val Gly Thr Val Ala Val Phe Leu Leu Lys Lys
50 55 60
Val Gly Ser Phe Ala Ala Lys Arg Ile Leu Asn Gly Leu Trp Asp Leu
65 70 75 80
Val Phe Pro Asn Asp Asn Thr Lys Leu Met Gln Asp Ile Leu Arg Glu
85 90 95
Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Ala Asp Thr Leu Ser Arg
100 105 110
Val Asn Ala Glu Leu Glu Gly Leu Gln Lys Asn Val Ala Glu Phe Asn
115 120 125
Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Pro Thr Leu Leu
130 135 140
Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn Arg
145 150 155 160
Leu Thr Gln Phe Gln Leu Arg Gly Tyr Glu Leu Leu Leu Leu Pro Leu
165 170 175
Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val Ile
180 185 190
Leu Asn Ala Glu Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr Tyr
195 200 205
Arg Asp Tyr Leu Lys Asp Tyr Thr Lys Glu Tyr Ser Asn Tyr Cys Ile
210 215 220
Asn Thr Tyr Gln Thr Ala Phe Lys Lys Leu Asn Thr Pro Leu His Glu
225 230 235 240
Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr Val
245 250 255
Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser Gly
260 265 270
Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Lys Gln Ser Phe
275 280 285
Ser Ala Gln Asn Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn Ser
290 295 300
Asn Tyr Val Leu Thr Gly Phe Ser Gly Ser Arg Ser Thr Val Lys Phe
305 310 315 320
Pro Gly Gly Gly Gly Leu Leu Pro Pro Gly Ser Thr Thr Thr Tyr Ala
325 330 335
Leu His Ser Ala Arg Ala Asn Tyr Lys Glu Gly Val Ser Ser Gly Ile
340 345 350
Ile Gly Gly Thr Asn Phe Asn His Asn Phe Lys Cys Arg Thr Ile Leu
355 360 365
Pro Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Thr
370 375 380
Asp Arg Glu Gly Val Thr Thr Phe Thr Asn Trp Gln Thr Glu Ala Phe
385 390 395 400
Glu Thr Thr Phe Gly Ile Arg Gly Gly Ala Phe Gly Pro Arg Gly Asn
405 410 415
Ala Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Leu Pro
420 425 430
Leu Val Val Arg Lys Glu Asp Leu Thr Lys Pro Leu His Tyr Asn Glu
435 440 445
Ile Lys Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala
450 455 460
Tyr Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Asp Ala His
465 470 475 480
Glu Asn Gly Thr Met Ile His Leu Ala Pro Asp Asp Asp Thr Gly Phe
485 490 495
Thr Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Ile Arg Thr
500 505 510
Phe Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu
515 520 525
Gln Thr Asn Thr Ala Ala Arg Tyr Thr Phe Arg Gly Asn Gly Asn Asn
530 535 540
Tyr Thr Leu Tyr Leu Arg Val Ser Ser Gln Gly Asn Ser Thr Ile Arg
545 550 555 560
Val Asn Ile Asn Gly Arg Val Phe Asn Val Pro Asn Val Asn Thr Thr
565 570 575
Thr Asn Asn Asp Gly Val Ile Asp Asn Gly Ala Arg Phe Ser Asp Ile
580 585 590
Arg Ile Gly Asn Ile Val Ala Ser Asn Asn Thr Asn Ile Pro Leu Asp
595 600 605
Ile Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Glu Leu Met Asn Ile
610 615 620
Met Phe Val Pro Asn Asn Leu Pro Pro Leu Tyr
625 630 635
<210> 216
<211> 360
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 216
Met Ala Glu Asn His Leu Glu Val Gln Gln Ile Asn Ser Gln Gln Glu
1 5 10 15
Arg Ala Ser Asp Thr Leu Ser Leu Ser Leu Arg Arg Val Gly Ser Asn
20 25 30
Ser Ala Leu Leu Gln Gly Tyr Gly Leu Val Ile Leu Gln Gln Ser Asn
35 40 45
Ile Thr Leu Pro Ala Met Gly Ser Leu Thr Thr His Gln Lys Ile Ala
50 55 60
Lys Thr Asn Val Lys Glu Trp Leu Asp Glu His Asn Pro Lys Leu Ile
65 70 75 80
Ala Leu Asn Gln Asp Ile Lys Lys Phe Ser Thr Lys Phe Asn Gly Tyr
85 90 95
Tyr Gln Thr Leu Tyr Asp Leu Ala Gly Gln Val Asn Glu Asn Glu Gln
100 105 110
Glu Lys Gln Asn Phe Glu Lys Gly Leu Ser Thr Leu Lys Asp Gln Gly
115 120 125
Gln Asn Thr Lys Ser Thr Met Asn Asp Thr Leu Ser Lys Phe Lys Asp
130 135 140
Phe Lys Thr Leu Leu Asp Asp Asp Val Glu Lys Phe Ser Thr Ala Ala
145 150 155 160
Ser Asp Ala Ile Gln Ser Val Gln Gly Pro Gly Gly Asp Ala Ile Gln
165 170 175
Leu Arg Glu Asn Ile Lys Lys Leu Gln Gln Asp Ile Gln Thr Glu Leu
180 185 190
Thr Asn Ile Leu Asn Arg Pro Ser Glu Ile Ile Asn Gly Ser Ile Asn
195 200 205
Phe Gly Lys Lys Val Tyr Thr Ile Gly Met Ser Ser Ala Glu Ser Gln
210 215 220
Thr Ile Asp Leu Val Ser Ile Gln Ser Leu Thr Gly Asp Leu Leu Asp
225 230 235 240
Leu Ser Asp His Lys Ile Lys Glu Ser Ala Asn Lys Ile Asp Ala Ile
245 250 255
Asn Asn Glu Leu Val Leu Leu Thr Lys Asn Leu Ser Asp Met Leu Ile
260 265 270
Gln Val Thr Gly Ile Thr Phe Ile Glu Asp Gln Val Thr Gly Phe Ala
275 280 285
Gly Met Ile Asp Arg Gln Ile Thr Thr Leu Glu Phe Leu Met Lys Asp
290 295 300
Trp Asp Val Ile Asn Glu Lys Ile Asn Gln Ile Gln Ser Asn Leu Asp
305 310 315 320
Ser Gly Ala Asn Ser Gly Leu Leu Lys Asp Gln Leu Val Glu Leu Lys
325 330 335
Lys Leu Asn Asp Glu Met Glu Asn Gln Ser Lys Gln Phe Glu Asp Phe
340 345 350
Leu Thr Val Glu Ile Thr Gln Ile
355 360
<210> 217
<211> 507
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 217
Met Asn Thr His Gly Lys Asn Glu Glu Trp Asn Gln His His Asp Gly
1 5 10 15
Thr Cys Gly Cys Gln Gln Asn Lys Gln Asp Tyr Gly Tyr Glu Gln Ile
20 25 30
Pro Glu His Asp Lys Gln Gln Tyr Ala Ser His Glu Tyr Lys Asn Gln
35 40 45
Glu Tyr Thr Tyr Lys Asp Ser Cys Gly Glu Ile Gly Thr Gly Tyr His
50 55 60
Asn Glu Asn Asn Gly Asp Phe Gln Cys Asp Thr Ile Arg Tyr Thr Thr
65 70 75 80
Pro Asn Leu Pro Val Glu Ser Thr Arg Phe Gln Thr Ile Thr Asn Ile
85 90 95
Asn Thr Asn Asp Asn Gly His Arg Val Met Asp Ala Met Asn Ile Ala
100 105 110
Val Gly Thr Gly Leu Glu Gln Arg Val Gly Gly Cys Pro Trp Gln Ile
115 120 125
Asp Val Glu Asn Thr Val Val Asn Glu Gln Val Arg Phe Asn Asn Gln
130 135 140
Ser Gln Leu Phe Val Phe Tyr Gln Thr Asp Ser Gly Gln Phe Val Ile
145 150 155 160
Ala Asn Arg Gly Asn Gly Arg Val Leu Glu Met Val Glu Phe Pro Asn
165 170 175
Ser Asp Pro Gln Trp His Gln Leu Leu Ile Ser Ser Phe Tyr Glu Asn
180 185 190
Arg Arg Asn Gln Leu Phe Asp Arg Arg Ile Ile Asn Asn Thr Asp Phe
195 200 205
Leu Leu Thr Thr Ile Thr Glu Thr Gly Ser Val Phe Gly Val Gln Asn
210 215 220
Cys Asn Asn Asn Pro Asn Ala Trp Gln Ile Tyr Thr Thr Val Asn Glu
225 230 235 240
Asn Glu Ser Val Gly Phe Arg Asn Asn Thr Tyr Arg Phe Arg Thr Glu
245 250 255
Asn Asn Leu Pro Ile Asn Ile Pro Pro Leu Arg Ser Gly Glu Pro Leu
260 265 270
Gly Pro Leu Pro Ala Leu Arg Tyr Leu Gln Asp Ser Gly Leu Ser Pro
275 280 285
Ala Glu Ala Pro Arg Ala Val Ile Gly Ser Ala Leu Leu Pro Ala Ile
290 295 300
Phe Val Glu Asp Val Leu Ser Leu Pro Ala Arg Met Ala Gln Ser Pro
305 310 315 320
Tyr Tyr Val Leu Glu Tyr Arg Gln Tyr Trp His Arg Leu Trp Ser Asp
325 330 335
Ile Ile Pro Ala Asn Gly Gln Ser Trp Phe Asn Glu Thr Thr Gly Met
340 345 350
Leu Gly Gly Ala Pro Gln Gln Gly Asn Met Arg Asn Thr Ile Ser Met
355 360 365
Ser Ile Ala Pro Asp Leu Gly Ile Arg Phe Gly Thr Gln Ser Met Pro
370 375 380
Phe Ser Ile Lys Ile Ala Glu Gly Leu Gly Arg Gln Arg Ser Asn Ala
385 390 395 400
Asn Ile Asp Ile Gly Glu Thr Ser Gln Asn Ile Arg Tyr Asp Asn Pro
405 410 415
Gln Asn Gln Pro Thr Arg Phe Ala Arg Phe Ala Leu Ala His Glu Tyr
420 425 430
Ala Leu Leu Arg Met Asp Gly Arg Val Ile Asn Arg Trp Thr Val Val
435 440 445
Asp Arg Ser Ser Met Tyr Leu Leu Ser Phe Pro Gln Asn Ala Leu Leu
450 455 460
Thr Met Gln Cys Asp Lys Ile Ile Arg Thr Asp Asn Ser Tyr Asp Leu
465 470 475 480
Ser Val Trp Lys Thr Pro Met Gln Ile Lys Ser Gly Gln Val Ile Thr
485 490 495
Lys Asn Glu Lys Asn Ser Lys Pro Tyr Asn Ala
500 505
<210> 218
<211> 457
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 218
Met Asp Leu Ser His Ala Asn His Asp Thr Asp Asn Gln Ile Tyr Lys
1 5 10 15
Thr Ser Cys Glu Cys Asn Gln Lys Thr Glu Trp Pro Met Ser Lys Lys
20 25 30
Gln Glu Gln Thr His Thr Asp Thr His Tyr Lys Ala Thr Gln Phe Asn
35 40 45
Ser Pro Ile Trp Asn Leu Pro Ile Glu Ser Arg Arg Phe Gln Thr Ile
50 55 60
Thr Asp Val Asn Ser Ser Gln Gly Arg Gly Leu Asn Ala Leu Arg Thr
65 70 75 80
Tyr Pro Gly Gln Leu Leu Gly Gly Asp Leu Gly Ala Phe Cys Arg Thr
85 90 95
Val Thr Leu Gln Asn Gln Val Val Asn Asp Leu Thr Arg Gly Asn Ile
100 105 110
Ala Arg Phe Leu Phe Tyr Asn Leu Asp Asn Gly Gly Phe Val Ile Ala
115 120 125
Asn Ala Asn Val Gly Glu Val Leu Gln Glu Met Asn Ala Asn Asn Ser
130 135 140
Ser Leu Ile Thr Asn Thr Tyr Thr Gly Gln Thr Asn Gln Phe Phe Arg
145 150 155 160
Lys Ser Pro Ile Thr Gln Asp His Phe Arg Leu Leu Thr Asn Glu Asn
165 170 175
Met Ile Val Gly Pro Cys Gly Asn Asn Ala Ser Asn Ser Trp Thr Arg
180 185 190
Ile Thr Leu Ala Leu Phe Ser Asn Gln Asn Phe Arg Tyr Arg His Glu
195 200 205
Pro Ser Glu Asn Gln Pro Ile Asn Leu Pro Gln Ala Ala Ala Thr Ser
210 215 220
Glu Leu Ala Pro Leu Pro Ala Leu Thr Ser Leu Asn Asp Pro Gly Pro
225 230 235 240
Ser Pro Val Val Ala Pro Arg Val Thr Ile Gly Ser Ala Leu Ile Pro
245 250 255
Cys Ile Phe Val Asn Asp Val Ile Pro Leu Gln His Arg Ile Arg Gln
260 265 270
Ser Pro Tyr Tyr Val Leu Glu Cys Arg Gln Tyr Trp His Arg Ile Trp
275 280 285
Ser Asp Val Leu Thr Ala Gly Ser Asp Ala Phe Tyr Glu Glu Arg Thr
290 295 300
Gly Ile Ser Asp Gly Ala Gln Ile Asp Ile Arg Asn Thr Met Asp Ile
305 310 315 320
Ser Ile Gly Ser Asp Trp Tyr Leu Arg Phe Gly Ser Leu Ser Ser Pro
325 330 335
Phe Arg Gly Gln Ile Ser Ser Gly Leu Arg Thr Gln Pro Ser Phe Ser
340 345 350
Asn Ile Asp Leu Ala Glu Thr Val Met Ile Asn Arg His Ile Asn Pro
355 360 365
Asn Ser Phe Thr Thr Arg Phe Ser Arg Phe Thr Lys Ala Ile Glu Tyr
370 375 380
Arg Leu Leu Arg Met Asp Gly Thr Val Val Arg Ser Trp Val Ala Val
385 390 395 400
Asp Asn Arg Ser Met Tyr Trp Lys Thr Phe Pro Asn Asn Ala Gln Ser
405 410 415
Thr Met Gln Ser Gln Lys Ile Ile Gln Ser Asp Thr Ser Tyr Asp Leu
420 425 430
Ser Val Trp Lys Thr Pro Met Val Ile Arg Asp Gly Glu Ile Ile Glu
435 440 445
Lys Asn Asp His Asn Ser Lys Thr Gln
450 455
<210> 219
<211> 948
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 219
Met Val Thr Lys Ala Met Thr Pro Lys Leu Gly Ala Leu Pro Asp Phe
1 5 10 15
Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Asp Asn Ile Thr Gly
20 25 30
Val Met Ser Thr Ile Phe Gly Val Asp Thr Gly Asp Ser Ser Ile Asp
35 40 45
Asn Val Leu Asn Asn Gln Glu Leu Leu Gln Glu Met Leu Asp Gln Met
50 55 60
Ser Thr Met Gln Ser Ser Ile Gln Asp Ile Leu Glu Gln Gln Gly Ile
65 70 75 80
Ser Ala Asp Ile Glu Lys Gln Ile Leu Ala Leu Thr Thr Asp Leu Ala
85 90 95
Thr Ser Ile Asn Thr Glu Leu Gly Lys Ile Glu Gly Ile Leu Asn Thr
100 105 110
Tyr Leu Pro Ala Ile Ser Asn Met Leu Ser Asn Ile Tyr Glu Gln Thr
115 120 125
Ser Leu Ile Asp Gln Lys Val Asp Lys Leu Leu Ala Leu Met Thr Phe
130 135 140
Ala Leu Lys Glu Leu Asp Tyr Ile Lys Asp Asn Val Val Leu Asn Ser
145 150 155 160
Ser Ile Val Glu Ile Thr Pro His Val Gln Lys Leu Val Tyr Val Asn
165 170 175
Lys Lys Phe Leu Ser Leu Thr Arg Gly Phe Leu Gln Gly Glu Asp Val
180 185 190
Ser Ile Asp Ser Met Gln Glu Ile Gln Glu Trp Ala Lys Ser Ile Leu
195 200 205
Ala Thr Glu Met Asn Ser Phe Glu Phe Ser Val Asp Thr Leu His Asn
210 215 220
Ile Ile Ile Gly Asp Asn Leu Tyr Lys Arg Ser Ala Leu Lys Thr Phe
225 230 235 240
Ser Asp Val Leu Leu Asp Asp Ala Asp Gln Tyr Gly Asp Phe Gly Thr
245 250 255
Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser Ser Leu Ala Thr Leu Gln
260 265 270
Ile Asn Ala Tyr Leu Cys Leu Thr Phe Ala Arg Lys Val Leu Gly Leu
275 280 285
Ser Glu Ile Asp Tyr Gln Val Thr Met Gln Asp Arg Ile Glu Gln Gln
290 295 300
Asn Gln Met Phe Val Asn Leu Ile Gln Asp Lys Asn Tyr Ser Asn Ala
305 310 315 320
Leu Glu Ile Thr Gly Ile Tyr Pro Met Ser Val Asp Trp Gly Asp Cys
325 330 335
Lys Ser Thr Asp Leu Gln Ala Asp Ala Gly Tyr Ala Leu Ile Gly Leu
340 345 350
Glu Phe Phe Met Asp Asn Gly Ile Tyr Lys Val Lys Ala Tyr Gln Gly
355 360 365
Lys Ile Gly Lys Asn Phe Ser Val Ser Gly Asp Thr Val Thr Glu Ile
370 375 380
Ile Ser Asp Asp Leu Ser Thr Val Phe Asn Asp Met Thr Asn Asp Asn
385 390 395 400
Pro Glu Leu Asp Val Val Tyr Pro Leu Ser Gly Glu Leu Thr Gly Pro
405 410 415
Pro Asn Thr Ile Ile Thr Arg Ile Gly Phe Gly Thr Lys Tyr Asp Lys
420 425 430
Thr Arg Glu Ser Ser Val Gln Ala Phe Ala Tyr Ile Asp Ala Asp Phe
435 440 445
Ser Pro Tyr Asp Tyr Ile Ser Gly Thr Ile Ser Lys Glu Gly Thr Gln
450 455 460
Thr Val Ser Leu Glu Gly Asn Asp His Lys Asn Arg Cys Tyr Ser Met
465 470 475 480
Trp Pro Ile Gly Leu Ile Gly Asp Leu Tyr Met Thr Pro Leu Lys Ser
485 490 495
Leu Ser Leu Asn Val Asp Val Asp Asp Ser Ser Ala Tyr Gly Thr Ile
500 505 510
Leu Asn Met Ser Gly Glu Ser Tyr Phe Ser Thr Ile Leu Ser Arg Glu
515 520 525
Tyr Asn Thr Asn Phe Ile Leu Phe Pro Tyr Thr Asn Asn Ser Gly Leu
530 535 540
Ile Asp Glu Asn Leu Ile Gln Asn Gly Asn Leu Glu Glu Gln Asp Lys
545 550 555 560
Tyr Trp Glu Gly Thr Glu Pro Ser Phe Phe Ala Glu Gly Glu Gly Thr
565 570 575
Phe Gly Ser Asn Ala Leu Lys Val Gln Ala Gln Gly Ala Phe Asp Gln
580 585 590
Ile Val Lys Leu Glu Pro Asn Thr Thr Tyr Arg Leu Thr Ala Phe Thr
595 600 605
Lys Val Lys Asp Ser Asn Tyr Ser Lys Gly Arg Ile Gly Ile Arg Asp
610 615 620
Met Tyr Asn Tyr Arg Val Glu Lys Ser Phe Leu His Thr Arg Tyr Lys
625 630 635 640
Gln Thr Lys Val Glu Phe Thr Thr Gly Ala Asp Thr Ser Lys Leu Ser
645 650 655
Val Phe Ile Gln Gly Gly Gly Asp Gln Glu Ser Ile Ala Trp Ala Asp
660 665 670
Asn Ile Glu Leu Tyr Lys Val His Gln Gln Asp Asn Met Ile Ser Asp
675 680 685
Phe Asp Phe Asp Thr Tyr Lys Tyr Ser Asn Met Cys Trp Lys Leu Ser
690 695 700
Gly Gly Gly Asp Phe Val Glu Lys Glu Gly Leu Phe Asn Ser Lys Ala
705 710 715 720
Leu Lys Ile Thr Asn Ser Gly Gly Ala Glu Gln Glu Val Lys Leu Lys
725 730 735
Ala Asn Thr Asn Tyr Ile Leu Thr Ala Tyr Val Lys Val Asp Val Pro
740 745 750
Thr Thr Thr Ala Gln Ile Gly Cys Gly Ser Asn Gln Val Pro Cys Asn
755 760 765
Ser Thr Ser Tyr Thr Pro Val Lys Leu Lys Phe Lys Thr Gly Glu Asp
770 775 780
Pro Ser Thr Thr Glu Ser Ser Val Tyr Cys Ser Asn Ser Asn Asp Ser
785 790 795 800
Gly Thr Val Trp Ala Asp Asn Phe Val Leu Tyr Glu Ala Pro Asn Leu
805 810 815
Ile Ile Asn Gly Asp Phe Glu Gln Phe Asp Gln Ser Ser Trp Asn Phe
820 825 830
Ser Pro Ser Glu Gly Gly Arg Ile Tyr Leu Gln Ser Gly Leu Gly Met
835 840 845
Ser Arg Ser Asn Ala Val Val Leu His Gly Gln Asn Gly Gln Ile Ser
850 855 860
Gln Lys Val Pro Leu Lys Pro Phe Thr Lys Tyr Arg Leu Thr Ala Tyr
865 870 875 880
Val Arg Val Ser Asp Gly Gly Thr Ala Trp Ile Gly Tyr Gly Asp Tyr
885 890 895
Gly Ala Ser Cys Thr Ser Lys Glu Phe Lys Gln Ile Ser Lys Asp Phe
900 905 910
Thr Thr Gly Ala Asn Pro Leu Gln Ser Asp Asp Ala Ile Tyr Leu Ser
915 920 925
Ser Asp Asn Ser Lys Tyr Thr Val Ala Asp Asn Phe Glu Leu Tyr Glu
930 935 940
Leu Asp Gln Ile
945
<210> 220
<211> 345
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 220
Met Ser Glu Ile Glu Gln Pro Ser Thr Gly Asp Met Ser Leu Ser Ala
1 5 10 15
Asn Glu Val Lys Met Lys Glu Ala Leu Gln Lys Ala Gly Leu Phe Ala
20 25 30
Lys Ser Met Asn Glu Tyr Ser Tyr Leu Leu Ile Asn Asn Pro Asp Val
35 40 45
Asn Phe Glu Gly Ile Thr Ile Asn Gly Tyr Ala Asp Leu Pro Asn Lys
50 55 60
Ile Val Gln Asp Gln Arg Asn Ala Arg Ala His Ala Val Thr Trp Asn
65 70 75 80
Thr Gln Val Lys Arg Gln Leu Leu Asp Thr Leu Thr Gly Ile Val Glu
85 90 95
Tyr Asp Thr Met Phe Asp Asn Tyr Tyr Asp Thr Ile Ile Asp Ala Ile
100 105 110
Asn Thr Gly Asp Gly Asp Thr Leu Lys Glu Gly Ile Thr Asp Leu Arg
115 120 125
Ser Glu Ile Gln Gln Asn Gln Lys Thr Ala Gln Asn Leu Ile Ala Glu
130 135 140
Leu Thr Lys Leu Arg Asp Asn Met Gly Glu Asp Val Arg Ala Phe Gly
145 150 155 160
Ser Asn Lys Glu Gln Leu Tyr Ser Ile Leu Lys Asn Gln Thr Ala Gly
165 170 175
Leu Glu Asp Asp Gln Lys Arg Leu Asp Glu Val Leu Gly Gln Val Asn
180 185 190
Tyr Tyr Lys Lys Leu Glu Ser Asp Gly Phe Thr Thr Met Lys Val Gly
195 200 205
Val Phe Gly Leu Trp Trp Ile Gly Gly Ile Ile Val Gly Thr Ala Arg
210 215 220
Asp Asn Leu Gly Lys Leu Glu Pro Tyr Leu Ala Glu Val Arg Gln Thr
225 230 235 240
Val Asp Asn Lys Thr Thr Leu Asn Arg Val Ile Glu Val Ala His Asn
245 250 255
Asn Thr Asp Gln Met His Lys Ser Ile Asp Asn Ala Ile Asn Ala Leu
260 265 270
Glu Tyr Met His Thr Gln Trp Thr Asp Leu Asp Asn Gln Tyr Ala Gly
275 280 285
Val Leu Gln Lys Ile Asp Asn Ala Ala Gly Lys Ala Asp Gln Asn Lys
290 295 300
Phe Ala Tyr Leu Lys Pro Thr Leu Lys Thr Ala Lys Asp Leu Trp Lys
305 310 315 320
Arg Leu Asn Glu Asp Ala Val Ile Ile Lys Glu Gly Ile Lys Glu Leu
325 330 335
Lys Val Glu Pro Val Thr Pro Gln Lys
340 345
<210> 221
<211> 264
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 221
Met Val Thr Lys Val Met Thr Pro Ser Leu Lys Ser Leu Pro Asp Phe
1 5 10 15
Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Gly Asn Ile Ser Asp
20 25 30
Ile Met Ser Thr Ile Phe Gly Ile Asp Val Gly Gly Asn Ser Asp Ile
35 40 45
Asp Asp Val Leu Asn Asn Ala Glu Leu Leu Gln Gln Met Met Asp Gln
50 55 60
Met Asn Ser Met Gln Gly Ser Leu Ala Asp Ile Leu Glu Glu Gln Gly
65 70 75 80
Met Ser Ile Glu Leu Ile Gln Lys Leu Thr Ser Leu Ser Asn Gln Gln
85 90 95
Leu Ala Leu Ser Thr Pro Ile Asn Thr Glu Leu Gly Lys Ile Glu Asp
100 105 110
Ile Leu Asn Asn Tyr Leu Pro Ala Ile Ser Asn Met Leu Asn Glu Val
115 120 125
Tyr His Glu Thr Val Gln Ile Asn Ala Lys Val Asp Lys Leu Leu Ser
130 135 140
Leu Met Met Tyr Ala Met Lys Glu Leu Asp Leu Ile Lys Asp Asn Val
145 150 155 160
Ile Leu Asn Ser Ser Val Val Glu Ile Thr Pro His Val Gln Lys Leu
165 170 175
Val Tyr Val Asn Lys Lys Phe Leu Glu Leu Ser Gly Asn Tyr Met Arg
180 185 190
Ser Asp Ile Ser Ile Asp Ser Met Gln Glu Leu Leu Gln Trp Ala Lys
195 200 205
Ser Ile Val Asp Thr Asp Met Asn Ser Phe Val Ser Thr Asp Thr Pro
210 215 220
Gly Asn Lys Val Phe Cys Ser Ile His Arg Ala Val Val Lys Ser Asn
225 230 235 240
Asn Ile Phe Gly Arg Ser Leu Cys Asn Gln Ala Arg Val Tyr Asn His
245 250 255
Leu Ile Arg Arg Gln Cys Pro Gly
260
<210> 222
<211> 288
<212> PRT
<213> 芽孢杆菌属的种
<400> 222
Met Ser Ile Tyr Asn Val Glu Ala Asp Leu Phe Asp Leu Cys Arg Glu
1 5 10 15
Tyr Ala Ile Lys His Tyr Asp Ala Asn Asp Ala Thr Phe His Gly Ala
20 25 30
Thr Ile Tyr Asp Arg Ser Val Leu Asn Ser Glu Val Met Pro Asp Gln
35 40 45
Val Glu Phe Asn Val Ile Pro Asp Leu Ser Asn Thr Val Ile Asn Thr
50 55 60
Ile Thr Asn Asp Thr Ser Ile Glu Gln Ser Met Ser Thr Lys Leu Ala
65 70 75 80
Thr Lys Thr Ile Glu Thr Thr Ser Phe Thr Thr Thr Glu Gly Tyr Lys
85 90 95
Ile Gly Thr Ser Ile Lys Asn Thr Leu Glu Phe Lys Ala Gly Gly Leu
100 105 110
Phe Ser Ile Gly Gly Val Glu His Lys Phe Glu Val Ser Val Ser Gly
115 120 125
Glu Tyr Asn His Ser Ser Ser Glu Thr Thr Ile Asn Thr Thr Glu Lys
130 135 140
Leu Trp Glu Tyr Thr Ala Pro Ile Lys Val Pro Pro Lys Thr Lys Val
145 150 155 160
Glu Gly Lys Ile Gln Ile Tyr Ser Gly Ala Ile Pro Val Pro Val Thr
165 170 175
Leu Lys Ser Thr Val Leu Gly Leu Gly Gln Ser Pro Asn Gly Lys Leu
180 185 190
Lys Asn Ala Leu Thr Ser Leu Trp Tyr Lys Asp Arg Ala Gly Lys Glu
195 200 205
Trp Val Asp Thr Tyr Gln Thr Ser His Leu Tyr Thr Leu Arg Glu Glu
210 215 220
Trp Ser Gly Tyr Lys Pro Ile Tyr Val Gly Ser Asp Gly Asn Gly Val
225 230 235 240
Lys Leu Gln Gly Lys Gly Thr Val Gly Leu Asp Leu Gly Leu Tyr Ala
245 250 255
Val Val Thr Phe Lys Glu Glu Pro Leu Ser Gly Asn Tyr Ser Met Gly
260 265 270
Asn Glu Arg Thr Tyr Ser Lys Val Ile Phe Arg Asp Gly Arg Val Ile
275 280 285
<210> 223
<211> 293
<212> PRT
<213> 芽孢杆菌属的种
<400> 223
Leu Leu Gly Gly Glu Thr Met Ile His Asp Leu Lys Glu Asp Ile Leu
1 5 10 15
Asp Phe Thr Lys Trp Tyr Ala Glu Lys His Tyr Asn Ala Asn Pro Asp
20 25 30
Thr Phe His Asp Glu Lys Val Phe Asp Pro Ser Val Ser Asn Ile Glu
35 40 45
Val Val Pro Asp Lys Ile Glu Phe Asp Thr Thr Pro Lys Leu Thr Ala
50 55 60
Arg Ala Thr Asp Ile Leu Ile Asn Asp Thr Ser Val Ser Gln Ser Ile
65 70 75 80
Thr Pro Lys Ile Ser Thr Lys Thr Val Glu Thr Thr Ser Ile Thr Thr
85 90 95
Thr Glu Gly Tyr Lys Ile Gly Ala Gly Ile Lys Tyr Thr Ser Thr Met
100 105 110
Lys Leu Lys Leu Phe Leu Ile Gly Glu Ile Ser Asn Ser Ile Glu Ile
115 120 125
Ala Val Thr Ser Glu Tyr Asn His Ser Thr Ser Glu Thr Gln Thr Asn
130 135 140
Thr Thr Glu Lys Ile Trp Glu Tyr Thr Arg Pro Val Ile Val Pro Pro
145 150 155 160
Arg Thr Lys Val Thr Ala Thr Leu Glu Val Tyr Ala Gly Pro Val Lys
165 170 175
Val Pro Val Thr Leu Arg Ser Thr Ile Thr Gly Met Gly Ile Ala Ser
180 185 190
Thr Gly Tyr Gly Tyr Ile Leu Gly Ser Val Ala Tyr Lys Asn Arg Gly
195 200 205
Gly Lys Ser Trp Ile Asp Ser Trp Arg Ser Arg Ser Leu Tyr Asp Phe
210 215 220
Arg Ser Thr Trp Pro Gly Phe Lys Pro Val Tyr Val Gly Thr Asp Gly
225 230 235 240
Glu Tyr Gly Val Lys Val Gln Gly Thr Ala Gln Val Glu Leu Glu Leu
245 250 255
Gly Leu Tyr Gly Val Val Thr Tyr Lys Glu Glu Pro Leu Phe Gly Asn
260 265 270
Asp Ser Asn Asn Ser Gln Thr Tyr Cys Glu Ile Ile Pro Arg Gln Gly
275 280 285
Leu His Thr Arg Ile
290
<210> 224
<211> 287
<212> PRT
<213> 人工序列
<220>
<223> 天然序列的合成变体
<400> 224
Met Ile His Asp Leu Lys Glu Asp Ile Leu Asp Phe Thr Lys Trp Tyr
1 5 10 15
Ala Glu Lys His Tyr Asn Ala Asn Pro Asp Thr Phe His Asp Glu Lys
20 25 30
Val Phe Asp Pro Ser Val Ser Asn Ile Glu Val Val Pro Asp Lys Ile
35 40 45
Glu Phe Asp Thr Thr Pro Lys Leu Thr Ala Arg Ala Thr Asp Ile Leu
50 55 60
Ile Asn Asp Thr Ser Val Ser Gln Ser Ile Thr Pro Lys Ile Ser Thr
65 70 75 80
Lys Thr Val Glu Thr Thr Ser Ile Thr Thr Thr Glu Gly Tyr Lys Ile
85 90 95
Gly Ala Gly Ile Lys Tyr Thr Ser Thr Met Lys Leu Lys Leu Phe Leu
100 105 110
Ile Gly Glu Ile Ser Asn Ser Ile Glu Ile Ala Val Thr Ser Glu Tyr
115 120 125
Asn His Ser Thr Ser Glu Thr Gln Thr Asn Thr Thr Glu Lys Ile Trp
130 135 140
Glu Tyr Thr Arg Pro Val Ile Val Pro Pro Arg Thr Lys Val Thr Ala
145 150 155 160
Thr Leu Glu Val Tyr Ala Gly Pro Val Lys Val Pro Val Thr Leu Arg
165 170 175
Ser Thr Ile Thr Gly Met Gly Ile Ala Ser Thr Gly Tyr Gly Tyr Ile
180 185 190
Leu Gly Ser Val Ala Tyr Lys Asn Arg Gly Gly Lys Ser Trp Ile Asp
195 200 205
Ser Trp Arg Ser Arg Ser Leu Tyr Asp Phe Arg Ser Thr Trp Pro Gly
210 215 220
Phe Lys Pro Val Tyr Val Gly Thr Asp Gly Glu Tyr Gly Val Lys Val
225 230 235 240
Gln Gly Thr Ala Gln Val Glu Leu Glu Leu Gly Leu Tyr Gly Val Val
245 250 255
Thr Tyr Lys Glu Glu Pro Leu Phe Gly Asn Asp Ser Asn Asn Ser Gln
260 265 270
Thr Tyr Cys Glu Ile Ile Pro Arg Gln Gly Leu His Thr Arg Ile
275 280 285

Claims (23)

1.一种具有杀虫活性的重组多肽，其包含：
(a)包含与选自SEQ ID NO:1或2中所示序列的氨基酸序列具有至少90％序列同一性百分比的氨基酸序列的多肽；或，
(b)包含SEQ ID NO:1或2中所示的氨基酸序列的多肽。
2.权利要求1的多肽，所述多肽进一步包含异源氨基酸序列。
3.一种组合物，其包含权利要求1或2的多肽。
4.一种编码氨基酸序列的重组核酸分子，所述氨基酸序列包含：
(a)与选自SEQ ID NO:1或2中所示序列的氨基酸序列具有至少90％的序列同一性百分比；或
(b)SEQ ID NO:1或2中所示的氨基酸序列；
其中所述重组核酸分子并非编码所述多肽的天然存在的序列。
5.权利要求4的重组核酸，其中所述核酸分子是设计用于在植物中表达的合成序列。
6.权利要求4或5的重组核酸分子，其中所述核酸分子与能够指导在植物细胞中的表达的启动子可操作地连接。
7.权利要求4-6中任一项的重组核酸分子，其中所述核酸分子与能够指导在细菌中的表达的启动子可操作地连接。
8.一种宿主细胞，其包含权利要求4-7中任一项的重组核酸分子。
9.权利要求8的宿主细胞，其中所述宿主细胞是细菌宿主细胞。
10.一种DNA构建体，其包含驱动植物细胞中的表达的启动子，所述启动子可操作地连接至重组核酸分子，所述重组核酸分子包含编码多肽的核苷酸序列，所述多肽包含与选自SEQ ID NO:1或2中所示的序列的氨基酸序列具有至少90％序列同一性百分比的氨基酸序列。
11.权利要求10的DNA构建体，其中所述核苷酸序列是设计用于在植物中表达的合成DNA序列。
12.一种载体，其包含权利要求10或11的DNA构建体。
13.一种宿主细胞，其包含权利要求10或11的DNA构建体或者权利要求12的载体。
14.一种组合物，其包含权利要求8、9或13中任一项的宿主细胞。
15.权利要求14的组合物，其中所述组合物选自粉末、粉剂、丸剂、颗粒剂、喷雾剂、乳剂、胶体和溶液。
16.权利要求14或15的组合物，其中所述组合物按重量计包含约1％至约99％的所述多肽。
17.一种用于控制害虫群体的方法，其包括使所述害虫群体与杀虫有效量的权利要求3或14-16中任一项的组合物接触。
18.一种用于产生具有杀虫活性的多肽的方法，其包括在其中编码所述多肽的核酸分子被表达的条件下，培养权利要求8、9或13中任一项的宿主细胞。
19.一种具有稳定整合到其基因组内的DNA构建体的植物，所述DNA构建体包含编码具有杀虫活性的蛋白质的核苷酸序列，其中所述核苷酸序列包含：
(a)编码包含SEQ ID NO:1或2中任何一个的氨基酸序列的多肽的核苷酸序列；或
(b)编码包含与选自SEQ ID NO:1或2中所示序列的氨基酸序列具有至少90％序列同一性百分比的氨基酸序列的多肽的核苷酸序列。
20.权利要求19的植物的转基因种子。
21.一种用于保护植物免受昆虫害虫的方法，其包括在植物或其细胞中表达编码杀虫多肽的核苷酸序列，其中所述核苷酸序列包含：
(a)编码包含SEQ ID NO:1或2中任何一个的氨基酸序列的多肽的核苷酸序列；或
(b)编码包含与选自SEQ ID NO:1或2中所示序列的氨基酸序列具有至少90％序列同一性百分比的氨基酸序列的多肽的核苷酸序列。
22.权利要求21的方法，其中所述植物产生针对鳞翅目害虫、鞘翅目害虫或半翅目害虫中的至少一种具有杀虫活性的杀虫多肽。
23.一种用于增加植物产量的方法，其包括在田间生长具有稳定整合到其基因组内的DNA构建体的植物或其种子，所述DNA构建体包含可操作地连接至编码杀虫多肽的核苷酸序列的启动子，所述启动子驱动在植物中的表达，其中所述核苷酸序列包含：
(a)编码包含SEQ ID NO:1或2中任何一个的氨基酸序列的多肽的核苷酸序列；或
(b)编码包含与选自SEQ ID NO:1或2中所示序列的氨基酸序列具有至少90％序列同一性百分比的氨基酸序列的多肽的核苷酸序列。
CN201680043643.1A 2015-06-22 2016-06-16 杀虫基因和使用方法 Pending CN107849097A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562182855P 2015-06-22 2015-06-22
US62/182,855 2015-06-22
PCT/US2016/037829 WO2016209708A1 (en) 2015-06-22 2016-06-16 Pesticidal genes and methods of use

Publications (1)

Publication Number Publication Date
CN107849097A true CN107849097A (zh) 2018-03-27

Family

ID=56511860

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680043643.1A Pending CN107849097A (zh) 2015-06-22 2016-06-16 杀虫基因和使用方法

Country Status (15)

Country Link
US (3) US20160366881A1 (zh)
EP (2) EP4276186A3 (zh)
KR (1) KR20180019668A (zh)
CN (1) CN107849097A (zh)
AR (1) AR105071A1 (zh)
AU (2) AU2016283985B2 (zh)
BR (1) BR112017027821A2 (zh)
CA (1) CA2989169A1 (zh)
CL (1) CL2017003319A1 (zh)
MA (1) MA49662A (zh)
MX (2) MX2017017146A (zh)
PH (2) PH12017502420A1 (zh)
RU (2) RU2021100887A (zh)
UA (1) UA125336C2 (zh)
WO (1) WO2016209708A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111793637A (zh) * 2020-07-24 2020-10-20 海口海森元生物科技有限公司 一种细菌磷脂酰肌醇特异性磷脂酶c基因及其应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019028235A2 (en) * 2017-08-03 2019-02-07 AgBiome, Inc. PESTICIDAL GENES AND METHODS OF USE

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010099365A2 (en) * 2009-02-27 2010-09-02 Athenix Corporation Pesticidal proteins and methods for their use
CN102066408A (zh) * 2008-06-25 2011-05-18 阿森尼克斯公司 毒素基因及其使用方法
CN103946393A (zh) * 2011-08-19 2014-07-23 合成基因组股份有限公司 用于高通量识别新型杀虫组合物的整合的方法及其用途

Family Cites Families (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4196265A (en) 1977-06-15 1980-04-01 The Wistar Institute Method of producing antibodies
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US4853331A (en) 1985-08-16 1989-08-01 Mycogen Corporation Cloning and expression of Bacillus thuringiensis toxin gene toxic to beetles of the order Coleoptera
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US5039523A (en) 1988-10-27 1991-08-13 Mycogen Corporation Novel Bacillus thuringiensis isolate denoted B.t. PS81F, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
US5240842A (en) 1989-07-11 1993-08-31 Biotechnology Research And Development Corporation Aerosol beam microinjector
ATE225853T1 (de) 1990-04-12 2002-10-15 Syngenta Participations Ag Gewebe-spezifische promotoren
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
CA2051562C (en) 1990-10-12 2003-12-02 Jewel M. Payne Bacillus thuringiensis isolates active against dipteran pests
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
CA2116449C (en) 1991-08-27 2005-04-05 Vaughan Alan Hilder Proteins with insecticidal properties against homopteran insects and their use in plant protection
EP0612208B1 (en) 1991-10-04 2004-09-15 North Carolina State University Pathogen-resistant transgenic plants
TW261517B (zh) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5428148A (en) 1992-04-24 1995-06-27 Beckman Instruments, Inc. N4 - acylated cytidinyl compounds useful in oligonucleotide synthesis
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
US5743477A (en) 1992-08-27 1998-04-28 Dowelanco Insecticidal proteins and method for plant protection
US5849870A (en) * 1993-03-25 1998-12-15 Novartis Finance Corporation Pesticidal proteins and strains
CZ290301B6 (cs) 1993-03-25 2002-07-17 Novartis Ag Pesticidní proteiny, kmeny které je obsahují, nukleotidové sekvence které je kódují a rostliny jimi transformované
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
US6063756A (en) 1996-09-24 2000-05-16 Monsanto Company Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor
PL336081A1 (en) 1997-04-03 2000-06-05 Novartis Ag Plant pests control
CA2315549A1 (en) 1998-02-26 1999-09-02 Pioneer Hi-Bred International, Inc. Family of maize pr-1 genes and promoters
ES2270227T3 (es) 1998-02-26 2007-04-01 Pioneer Hi-Bred International, Inc. Promotor met-1 del maiz.
ATE309362T1 (de) 1998-08-20 2005-11-15 Pioneer Hi Bred Int Samen-bevorzugende promotoren
WO2000012733A1 (en) 1998-08-28 2000-03-09 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from end genes
US6468523B1 (en) 1998-11-02 2002-10-22 Monsanto Technology Llc Polypeptide compositions toxic to diabrotic insects, and methods of use
EP1173578A2 (en) 1999-05-04 2002-01-23 Monsanto Company Coleopteran-toxic polypeptide compositions and insect-resistant transgenic plants
CN1390259A (zh) 1999-09-15 2003-01-08 孟山都技术有限公司 对鳞翅目昆虫有活性的苏云金芽孢杆菌δ内毒素组合物及其使用方法
EP1250447B1 (en) 1999-11-29 2011-12-21 Midwest Oilseeds, Inc. Methods, media and apparatus for the introduction of molecules into plant cells and bacteria using aerosol beams
US8796026B2 (en) * 2003-07-07 2014-08-05 Monsanto Technology Llc Insecticidal proteins secreted from Bacillus thuringiensis and uses therefor
US20050183161A1 (en) 2003-10-14 2005-08-18 Athenix Corporation AXMI-010, a delta-endotoxin gene and methods for its use
WO2005066202A2 (en) 2003-12-22 2005-07-21 E.I. Du Pont De Nemours And Company Bacillus cry9 family members
US7473822B1 (en) 2005-06-27 2009-01-06 Iowa State University Research Foundation, Inc. Soybean transformation and regeneration using half-seed explant
NZ573398A (en) 2006-06-14 2011-11-25 Athenix Corp Axmi-031, axmi-039, axmi-040 and axmi-049, a family of delta-endotoxin genes and methods for their use
ES2864641T3 (es) 2006-06-15 2021-10-14 BASF Agricultural Solutions Seed US LLC Una familia de proteínas pesticidas y procedimientos para su uso
HUE032365T2 (en) 2009-07-02 2017-09-28 Athenix Corp AXMI-205 pesticide gene and methods of application
AR077344A1 (es) * 2009-07-31 2011-08-17 Athenix Corp Familia de genes plaguicidas axmi-192 y metodos para su uso
US8586832B2 (en) 2009-12-21 2013-11-19 Pioneer Hi Bred International Inc Bacillus thuringiensis gene with Lepidopteran activity
MX2013001742A (es) 2010-08-19 2013-05-14 Pioneer Hi Bred Int Nuevo gen de bacillus thuringiensis con actividad lepidoptera
MX2013001741A (es) * 2010-08-20 2013-05-14 Pioneer Hi Bred Int Nuevo gen de bacillus thuringiensis con actividad coleoptera
US8878007B2 (en) 2011-03-10 2014-11-04 Pioneer Hi Bred International Inc Bacillus thuringiensis gene with lepidopteran activity
BR122014004140B8 (pt) 2011-08-22 2023-03-28 Bayer Cropscience Ag Vetor recombinante ou construção recombinante, bem como métodos para obter e produzir uma planta de algodão ou célula vegetal tolerante a um inibidor de hppd, e para cultivar um campo de plantas de algodão
EP2822963A2 (en) * 2012-03-09 2015-01-14 Vestaron Corporation Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides
US9475847B2 (en) * 2012-07-26 2016-10-25 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN102796183B (zh) * 2012-08-02 2013-12-18 北京大北农科技集团股份有限公司 杀虫蛋白质、其编码基因及用途

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102066408A (zh) * 2008-06-25 2011-05-18 阿森尼克斯公司 毒素基因及其使用方法
WO2010099365A2 (en) * 2009-02-27 2010-09-02 Athenix Corporation Pesticidal proteins and methods for their use
CN103946393A (zh) * 2011-08-19 2014-07-23 合成基因组股份有限公司 用于高通量识别新型杀虫组合物的整合的方法及其用途

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHENGCHEN XU, ET AL.: "Structural Insights into Bacillus thuringiensis Cry, Cyt and Parasporin Toxins", 《TOXINS》 *
丁勇: "《基因工程与农业》", 31 December 1994, 科学技术文献出版社 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111793637A (zh) * 2020-07-24 2020-10-20 海口海森元生物科技有限公司 一种细菌磷脂酰肌醇特异性磷脂酶c基因及其应用
CN111793637B (zh) * 2020-07-24 2023-04-14 海口海森元生物科技有限公司 一种细菌磷脂酰肌醇特异性磷脂酶c基因及其应用

Also Published As

Publication number Publication date
US20220272974A1 (en) 2022-09-01
RU2018102090A3 (zh) 2019-12-27
CL2017003319A1 (es) 2018-06-22
MA49662A (fr) 2020-06-03
MX2021003721A (es) 2021-06-08
MX2017017146A (es) 2018-02-23
PH12017502420A1 (en) 2018-07-02
US20160366881A1 (en) 2016-12-22
CA2989169A1 (en) 2016-12-29
AR105071A1 (es) 2017-09-06
AU2016283985B2 (en) 2021-01-21
WO2016209708A1 (en) 2016-12-29
US20190307125A1 (en) 2019-10-10
RU2021100887A3 (zh) 2022-01-21
UA125336C2 (uk) 2022-02-23
PH12021550691A1 (en) 2022-02-21
RU2021100887A (ru) 2021-03-16
AU2016283985A1 (en) 2018-01-18
EP4276186A3 (en) 2024-03-06
EP3310802A1 (en) 2018-04-25
EP4276186A2 (en) 2023-11-15
AU2021202218A1 (en) 2021-05-06
KR20180019668A (ko) 2018-02-26
BR112017027821A2 (pt) 2019-05-14
RU2742467C2 (ru) 2021-02-08
RU2018102090A (ru) 2019-07-22

Similar Documents

Publication Publication Date Title
US10421976B2 (en) Pesticidal genes and methods of use
CN108064234A (zh) 杀虫基因和使用方法
US11898153B2 (en) Pesticidal genes and methods of use
US11453890B2 (en) Pesticidal genes and methods of use
US10793610B2 (en) Pesticidal genes and methods of use
US20220272974A1 (en) Pesticidal genes and methods of use
US20190316147A1 (en) Pesticidal Genes and Methods of Use
US20210108224A1 (en) Pesticidal genes and methods of use
BR112020021445A2 (pt) Genes pesticidas e métodos de uso
US11780890B2 (en) Pesticidal genes and methods of use
CN107438617A (zh) 杀虫基因和使用方法
EP4230642A2 (en) Pesticidal genes and methods of use

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination