CN107843595B - Mould test method - Google Patents
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- CN107843595B CN107843595B CN201711002316.4A CN201711002316A CN107843595B CN 107843595 B CN107843595 B CN 107843595B CN 201711002316 A CN201711002316 A CN 201711002316A CN 107843595 B CN107843595 B CN 107843595B
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
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Abstract
The invention relates to a mould test method, which can obtain the area proportion of mould erosion of a test sample before and after a mould test by applying a high-imaging photographic system to carry out imaging analysis on a mould growing part of the test sample before and after the mould test, and can obtain the variety of a grown strain by carrying out comparative analysis on the color and the shape of the hypha of the grown strain. Therefore, the mould test method can reduce the uncertainty of the mould test, improve the accuracy and the reliability of the mould test, and is beneficial to the mould test to form a unified and effective test result judgment mechanism.
Description
Technical Field
The invention relates to a mould test method.
Background
The mold test is used to test the ability of a sample to resist mold and to determine whether the sample will be adversely affected by mold under conditions conducive to mold growth (i.e., in a humid and warm environment and in the presence of inorganic salts). However, in actual operation, the judgment of the mildew growth condition of the sample in the mildew test is different due to different subjectivity of testers, and a unified and effective test result judgment mechanism cannot be formed.
Based on the above situation, it is necessary to provide a determination process capable of being microscopically visualized for the mold test result.
Disclosure of Invention
The invention aims to provide a mould test method, which can solve the problem that the judgment result of the mould test cannot be microscopic visualized and quantized, so that the mould test result is perfected, and the accuracy of the mould test is improved.
In order to achieve the purpose, the invention provides the following technical scheme: a method of testing for mold comprising the steps of:
s1, providing a strain and mould test box, carrying out primary treatment on the strain, and preparing the strain into a mixed spore suspension; the preliminary treatment comprises strain inspection, culture medium preparation, mould spore activity inspection, strain separation, transplantation, culture and preservation;
and S2, providing a test sample and a control sample, detecting the test sample and the control sample and recording relevant characteristics, wherein the relevant characteristics comprise the smoothness, the defects and the material process of the sample.
S3, preprocessing the test sample and the control sample, and shooting and archiving the preprocessed test sample and the preprocessed control sample through a high-imaging photographic system to obtain a first test sample image and a first control sample image;
s4, placing the test sample and the control sample in the mold test box, spraying the mixed spore suspension on the test sample and the control sample, closing a box door of the mold test box, and starting a mold test;
s5, after the mould test is finished, detecting the test sample and the control sample through the high-imaging photographic system, recording related data, observing and calculating the area proportion of the test sample and the control sample corroded by the mould, and shooting and archiving to obtain a second test sample image and a second control sample image; the related data comprise growth parts, coverage areas, colors, growth forms, growth densities and growth thicknesses of the moulds;
s6, analyzing the result of the mold test by comparing the first test sample graph and the second test sample graph and the first control sample graph and the second control sample graph.
Further, in step S3, the preprocessing specifically includes: the test and control samples were washed with a wash solution, then wiped and air dried.
Further, the cleaning solution is selected from 75% alcohol or 95% alcohol or distilled water or water containing detergent.
Further, the mold test was performed 72 hours after the end of the pretreatment.
Further, in step S4, the test sample and the control sample are placed at the same height of the mold test chamber, and there is a space between the test sample and the control sample.
Further, in step S4, during the mold test, the mold test chamber is ventilated every seven days.
Further, the mold test method further comprises performance judgment, wherein the performance judgment specifically comprises the following steps:
in step S2, detecting electrical and mechanical properties of the test sample;
in step S5, after the mold test is finished, the test sample is left in the mold test chamber under the temperature and humidity condition for electrical and mechanical property detection; or, at the end of the mold test, taking the test sample out of the mold test chamber, and carrying out electrical property and mechanical property detection after the test sample is placed for 24 hours under standard atmospheric conditions.
Further, the mould test method also comprises a test reliability judging step, wherein the test reliability judging step specifically comprises the following steps:
on the seventh day of the mold test, checking the control sample for mold growth from the window of the mold test chamber, and if the mold coverage is greater than or equal to 90%, continuing the mold test; stopping and restarting the mold test if the mold coverage is less than 90%;
on the seventh day of the mold test, performing a mold spore viability test on the strain cultured and preserved in step S1, and if the strain grows normally, continuing the mold test; and if the strains can not grow normally, stopping the mould test and reselecting the strains.
Further, the test reliability judgment further includes:
in step S5, if the area proportion of the control sample that is attacked by mold is smaller than the area proportion of the control sample that is attacked by mold at the seventh day of the mold test, the mold test is stopped and restarted.
Further, the high-imaging photographic system is a microscopic camera.
The invention has the beneficial effects that: the mould test method of the invention can obtain the area proportion of mould erosion of the test sample before and after the mould test by applying the high-imaging camera system to carry out imaging analysis on the mould growing part of the test sample before and after the mould test, and can obtain the variety of the grown strain by carrying out comparative analysis on the color and the shape of the hypha of the grown strain. Therefore, the mould test method can reduce the uncertainty of the mould test, improve the accuracy and the reliability of the mould test, and is beneficial to the mould test to form a unified and effective test result judgment mechanism.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood and to implement them in accordance with the contents of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings.
Drawings
FIG. 1 is a flow chart of a method for testing mold in accordance with one embodiment of the present invention;
FIG. 2 is a photomicrograph of a test sample according to one embodiment of the present invention.
Detailed Description
The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Referring to fig. 1, the mold testing method of the present invention comprises the following steps:
s1, providing a strain and mould test box, carrying out primary treatment on the strain, and preparing the strain into a mixed spore suspension; the preliminary treatment comprises strain inspection, culture medium preparation, mould spore activity inspection, strain separation, transplantation, culture and preservation;
s2, providing a test sample and a control sample, detecting the test sample and the control sample and recording relevant characteristics, wherein the relevant characteristics comprise the smoothness, defects and material process of the sample;
s3, preprocessing the test sample and the control sample, namely cleaning the test sample and the control sample by using a cleaning solution, then wiping and airing, and then shooting and archiving the preprocessed test sample and the preprocessed control sample by using a high-imaging photographic system to obtain a first test sample image and a first control sample image;
s4, after the pretreatment is finished for 72 hours, placing the test sample and the control sample in the mold test box, wherein in order to ensure the accuracy of the mold test, the test sample and the control sample are placed at the same height of the mold test box, and a space exists between the test sample and the control sample; spraying the mixed spore suspension on the test sample and the control sample, closing a box door of the mold test box, and starting a mold test, wherein the mold test box is subjected to ventilation treatment every seven days in the process of the mold test;
s5, after the mould test is finished, detecting the test sample and the control sample through the high-imaging photographic system, recording related data, observing and calculating the area proportion of the test sample and the control sample corroded by the mould, and shooting and archiving to obtain a second test sample image and a second control sample image; the related data comprise growth parts, coverage areas, colors, growth forms, growth densities and growth thicknesses of the moulds;
and S6, comparing the first test sample image with the second test sample image and the first control sample image with the second control sample image to analyze the result of the mold test, namely, performing imaging analysis on the mold growing part of the test sample before and after the mold test to obtain the area proportion of mold erosion of the test sample before and after the mold test, and comparing and analyzing the color and shape of the grown strain hyphae to obtain the variety of the grown strain.
In order to ensure the accuracy of the mould test method, the mould test method further comprises a test reliability judging step, wherein the test reliability judging step specifically comprises the following steps:
on the seventh day of the mold test, checking the control sample for mold growth from the window of the mold test chamber, and if the mold coverage is greater than or equal to 90%, continuing the mold test; stopping and restarting the mold test if the mold coverage is less than 90%;
on the seventh day of the mold test, performing a mold spore viability test on the strain cultured and preserved in step S1, and if the strain grows normally, continuing the mold test; and if the strains can not grow normally, stopping the mould test and reselecting the strains.
In step S5, if the mold-eroded area proportion of the control sample is smaller than the mold-eroded area proportion of the control sample at the seventh day of the mold test, the mold test is stopped and restarted.
In this embodiment, the mold testing method further comprises performance determination:
in step S2, detecting electrical and mechanical properties of the test sample;
in step S5, after the mold test is finished, the test sample is left in the mold test chamber under the temperature and humidity condition for electrical and mechanical property detection; or, at the end of the mold test, taking the test sample out of the mold test chamber, and carrying out electrical property and mechanical property detection after the test sample is placed for 24 hours under standard atmospheric conditions.
Indeed, in other embodiments, the electrical and mechanical properties of the test sample may be selectively measured depending on the application.
In this embodiment, in step S3, the cleaning solution is 75% alcohol, and indeed, in other embodiments, the cleaning solution may be 95% alcohol or distilled water or water containing detergent. In this embodiment, the high-imaging photographing system is a microscope camera, and indeed, in other embodiments, the high-imaging photographing system may also be selected according to actual needs.
Referring to fig. 2, it can be seen that the test samples of this example have obvious hypha and spore growth on the surface of the sample after the mold test, but the substrate does not have obvious corrosion. However, in the conventional mold test, only the entire appearance of the test sample is usually photographed and described, and whether the substrate is corroded in the standard determination cannot be determined by visual observation or photographing with a general camera. Meanwhile, whether the mold grows well or not has no relevant photo support as evidence, and when a full-view picture is directly taken, the specific growth condition of the mold cannot be determined due to different uniformity of spraying spore suspension.
In summary, the following steps: the mould test method of the invention can obtain the area proportion of mould erosion of the test sample before and after the mould test by applying the high-imaging camera system to carry out imaging analysis on the mould growing part of the test sample before and after the mould test, and can obtain the variety of the grown strain by carrying out comparative analysis on the color and the shape of the hypha of the grown strain. Therefore, the mould test method can reduce the uncertainty of the mould test, improve the accuracy and the reliability of the mould test, and is beneficial to the mould test to form a unified and effective test result judgment mechanism.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (9)
1. A method for testing mold, comprising the steps of:
s1, providing a strain and mould test box, carrying out primary treatment on the strain, and preparing the strain into a mixed spore suspension; the preliminary treatment comprises strain inspection, culture medium preparation, mould spore activity inspection, strain separation, transplantation, culture and preservation;
s2, providing a test sample and a control sample, detecting the test sample and the control sample and recording relevant characteristics, wherein the relevant characteristics comprise the smoothness, defects and material process of the sample; and detecting the electrical and mechanical properties of the test sample;
s3, preprocessing the test sample and the control sample, and shooting and archiving the preprocessed test sample and the preprocessed control sample through a high-imaging photographic system to obtain a first test sample image and a first control sample image;
s4, placing the test sample and the control sample in the mold test box, spraying the mixed spore suspension on the test sample and the control sample, closing a box door of the mold test box, and starting a mold test;
s5, after the mould test is finished, detecting the test sample and the control sample through the high-imaging photographic system, recording related data, observing and calculating the area proportion of the test sample and the control sample corroded by the mould, and shooting and archiving to obtain a second test sample image and a second control sample image; the related data comprise growth parts, coverage areas, colors, growth forms, growth densities and growth thicknesses of the moulds;
after the mould test is finished, the test sample is left in the mould test box under the temperature and humidity condition for electrical property and mechanical property detection; or when the mould test is finished, taking the test sample out of the mould test box, and carrying out electrical property and mechanical property detection after the test sample is placed for 24 hours under the standard atmospheric condition;
s6, analyzing the result of the mold test by comparing the first test sample graph and the second test sample graph and the first control sample graph and the second control sample graph.
2. The mold testing method according to claim 1, wherein in step S3, the pretreatment is specifically: the test and control samples were washed with a wash solution, then wiped and air dried.
3. The mold test method according to claim 2, wherein the cleaning solution is selected from 75% alcohol or 95% alcohol or distilled water or water containing a detergent.
4. The mold test method of claim 1, wherein the mold test is performed 72 hours after the end of the pretreatment.
5. The mold testing method of claim 4, wherein in step S4, the test sample and the control sample are placed at the same height of the mold test chamber with a space between the test sample and the control sample.
6. The mold testing method of claim 5, wherein in step S4, the mold testing chamber is ventilated every seven days during the mold test.
7. The mold testing method according to claim 1, further comprising a test reliability judging step, wherein the test reliability judging step is specifically:
on the seventh day of the mold test, checking the control sample for mold growth from the window of the mold test chamber, and if the mold coverage is greater than or equal to 90%, continuing the mold test; stopping and restarting the mold test if the mold coverage is less than 90%;
on the seventh day of the mold test, performing a mold spore viability test on the strain cultured and preserved in step S1, and if the strain grows normally, continuing the mold test; and if the strains can not grow normally, stopping the mould test and reselecting the strains.
8. The mold test method of claim 7, wherein the test reliability determination further comprises:
in step S5, if the area proportion of the control sample that is attacked by mold is smaller than the area proportion of the control sample that is attacked by mold at the seventh day of the mold test, the mold test is stopped and restarted.
9. The mold test method according to any one of claims 1 to 8, characterized in that the high-imaging camera system is a microscopic camera.
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