CN107254505A - A kind of evaluation method of material sample block fungicidal properties - Google Patents
A kind of evaluation method of material sample block fungicidal properties Download PDFInfo
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- CN107254505A CN107254505A CN201710582354.5A CN201710582354A CN107254505A CN 107254505 A CN107254505 A CN 107254505A CN 201710582354 A CN201710582354 A CN 201710582354A CN 107254505 A CN107254505 A CN 107254505A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
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Abstract
The invention discloses a kind of evaluation method of material sample block fungicidal properties, comprise the following steps：S100：Mould is cultivated on material sample block；S200：Measure the area of mold colony on material sample block；S300：Calculate the ratio of the mold colony area and material sample block area；S400:The ratio of the mold colony area and material sample block area is bigger, and the fungicidal properties of material sample block is poorer.The present invention cultivates mould using unified condition of culture on material sample block first, after the time that culture mould is specified, calculates the surface area fraction of mould surface area and material sample block, the power of reaction material sample block fungicidal properties is just capable of according to the ratio size.
Description
Technical field
The present invention relates to a kind of evaluation method of material sample block fungicidal properties, belong to inspection, detection technique field.
Background technology
Mould, is being commonly called as filamentous fungi, implies that " mouldy fungi ", and they tend to form branch mycelia in great numbers
Body, but large-scale fructification is produced not as mushroom.Some are grown on the place of damp warm, many articles visually may be used
The villiform seen, cotton-shaped or arachnoid bacterium colony.Mould is ubiquitous in our life, and he, which compares, favors in warm moist
Environment, one has suitable environment substantial amounts of will breed, however at present not article fungicidal properties evaluation method, institute
To become urgent problem to be solved there is provided a kind of evaluation method of fungicidal properties.
The content of the invention
It is an object of the present invention to provide a kind of evaluation method of material sample block fungicidal properties, being capable of section using this method
Evaluation material sample block fungicidal properties, confirm material under certain conditions whether can mould growth, and growth it is mould
The size of bacterium bacterium colony.
In order to solve the above technical problems, the present invention is adopted the following technical scheme that：
A kind of evaluation method of material sample block fungicidal properties, comprises the following steps：S100：Cultivate mould on material sample block
Bacterium；S200：Measure the area and the area of material sample block of mold colony on material sample block；S300：Calculate the mold colony face
Product and the ratio of material sample block area；S400:The ratio of the mold colony area and material sample block area is bigger, material sample block
Fungicidal properties it is poorer.
In a kind of evaluation method of foregoing material sample block fungicidal properties, the step S200 also includes following methods：Will
Camera lens and etalon keep distance to a declared goal A, and etalon is taken pictures, and determining should in etalon unit sizes and photo
The proportionate relationship of unit sizes respective number of pixels；Camera lens and material sample block are kept at a distance A, material sample block is taken pictures, it is right
Mold colony on the block of material sample described in photo is demarcated, and determines the scope of mold colony on material sample block；To on photo
Pixel is counted in the mold colony area of demarcation, and material sample block in photo is counted comprising area respective number of pixels；
The real area of mold colony and the real area of material sample block are calculated according to count results and the proportionate relationship.
In a kind of evaluation method of foregoing material sample block fungicidal properties, in addition to following methods：According on material sample block
The form of mold colony manually draws a circle to approve the scope of the mold colony.
In a kind of evaluation method of foregoing material sample block fungicidal properties, in addition to following methods：One square of the photo
Millimeter includes the pixel of more than 100.
In a kind of evaluation method of foregoing material sample block fungicidal properties, in addition to following methods：Using unified standard
Mould is cultivated on material sample block.
In a kind of evaluation method of foregoing material sample block fungicidal properties, adjust single on material sample block by adjusting video camera
The corresponding number of pixels of unit area is more on the corresponding number of pixels of plane product, material sample block, mold colony on material sample block
The result of calculation of area is more accurate.
In a kind of evaluation method of foregoing material sample block fungicidal properties, camera lens is clapped etalon in recording step S200
According to when lens focus and the proportionate relationship, material sample block and camera lens is kept at a distance when being taken pictures to another material sample block
A, lens focus when being taken pictures using camera lens in S200 to etalon；To the mold colony on material sample block described in the photo
Demarcated, pixel in the mold colony area demarcated on the photo is counted, face is included to material sample block in the photo
Product respective number of pixels is counted；The real area and material of the mold colony are calculated according to count results and the proportionate relationship
Expect the real area of sample block.
Compared with prior art, the present invention cultivates mould using unified condition of culture on material sample block first, cultivates
After the time that mould is specified, the surface area fraction of mould surface area and material sample block is calculated, just can according to the ratio size
The power of reaction material sample block fungicidal properties.
Brief description of the drawings
Fig. 1 is to realize the device used in this evaluation method.
Embodiment
Embodiments of the invention 1：The present embodiment illustrates the evaluation method of the present invention in specific implementation by taking air-conditioning panel as an example
During method, step.
Generally cooled in temperature higher season using air-conditioning, vapor while cooling in air is due to temperature
Reduction condenses into droplet and is covered on air-conditioning panel, and easy dust stratification on air-conditioning panel, does not clear up easy growth for a long time
Mould.
First, one piece of air-conditioning panel is taken；For the ease of subsequent operation, air-conditioning panel can also be cut into the shape of rule
Shape, such as：It is circular, square etc..Then mould is cultivated on air-conditioning panel using conventional method, the method for culture mould will be kept
It is highly consistent, such as：Temperature, humidity, time, type selecting of culture medium etc., are required for unanimously, in order to compare, distinguish any material
The Anti-mildew capability of material is more preferable.
After the culture for completing mould, the area and the area of material sample block of mold colony, calculating side on measurement material sample block
Method is as follows：The camera lens of video camera and etalon are kept into distance to a declared goal A, etalon is taken pictures, etalon is determined
The proportionate relationship of the unit sizes respective number of pixels in unit sizes and photo；Then camera lens and material sample block are kept at a distance A,
Material sample block is taken pictures, then the artificial scope for drawing a circle to approve mold colony in photo, can pass through mold colony and material sample
The form difference of block is drawn a circle to approve, and because mould is grown on air-conditioning panel, air-conditioning panel surface is smooth, and mold colony
There is obvious difference with the configuration of surface of air-conditioning panel, so by naked eyes it may determine that going out the growth scope of mould.Control
Pixel is counted in the mold colony area drawn a circle to approve on piece, and material sample block in photo is counted comprising area respective number of pixels
Number；Then the area of mold colony on material sample block is calculated by the pixel count of mold colony in photo and the proportionate relationship,
The area of material sample block is calculated by the pixel count of material sample block in photo and the proportionate relationship.Calculate the reality of mold colony
After interphase product, it is possible to calculate the ratio of the mold colony area and material sample block area, the bigger explanation air-conditioning panel of ratio
Upper fungus growth it is faster, illustrate that the fungicidal properties of air-conditioning panel is poorer；Conversely, mould life on the smaller explanation air-conditioning panel of ratio
Long is slower, illustrates that the fungicidal properties of air-conditioning panel is stronger.
100 pixels are included it is worth noting that, shooting the photograph come and need to keep 1 square millimeter, if pixel is too
Follow-up measurement accuracy is influenceed at least, is caused the mold colony area calculated inaccurate, is caused the evaluation method of the present invention not
It is enough accurate.Counting to pixel in photo can quickly be obtained by photo handling software.
Embodiment 2：The present embodiment is carried out after embodiment 1 is completed, it has been determined that etalon unit sizes and photograph
The proportionate relationship of the unit sizes respective number of pixels of this in piece.First, one piece of air-conditioning panel is taken；For the ease of subsequent operation, also may be used
So that air-conditioning panel to be cut into the shape of rule, such as：It is circular, square etc..Then trained using conventional method on air-conditioning panel
Mould is supported, the method for culture mould will keep highly consistent, such as：Temperature, humidity, time, type selecting of culture medium etc., are required for one
Cause, in order to which the Anti-mildew capability for comparing, distinguishing any material is more preferable.
After the culture for completing mould, the area and the area of material sample block of mold colony, calculating side on measurement material sample block
Method is as follows：Camera lens and material sample block are kept at a distance A, material sample block is taken pictures, then mould bacterium in artificial delineation photo
The scope fallen, can be drawn a circle to approve by the way that mold colony is different with the form of material sample block, because mould is to be grown in conditioned surface
On plate, air-conditioning panel surface is smooth, and mold colony and the configuration of surface of air-conditioning panel have obvious difference, so passing through meat
Eye is it may determine that go out the growth scope of mould.Pixel in the mold colony area drawn a circle to approve on photo is counted, to photo
Middle material sample block is counted comprising area respective number of pixels；Then the pixel count and embodiment 1 of mold colony in photo are passed through
The proportionate relationship calculates the area of mold colony on material sample block, passes through the pixel count and embodiment 1 of material sample block in photo
The proportionate relationship calculates the area of material sample block.Compared with embodiment 1, because the distance of camera lens and material sample block is unchanged, and
And video camera is not focused, so the proportionate relationship is consistent, this method is applied to the material similar to size
Material sample block is measured.
After the real area for calculating mold colony, it is possible to calculate the mold colony area and material sample block area
Fungus growth is faster on ratio, the bigger explanation air-conditioning panel of ratio, illustrates that the fungicidal properties of air-conditioning panel is poorer；Conversely, than
Fungus growth is slower on the smaller explanation air-conditioning panel of value, illustrates that the fungicidal properties of air-conditioning panel is stronger.
Claims (7)
1. a kind of evaluation method of material sample block fungicidal properties, it is characterised in that comprise the following steps：S100：In material sample block
Upper culture mould；S200：Measure the area and the area of material sample block of mold colony on material sample block；S300：Calculate described mould
Bacterium area of colony and the ratio of material sample block area；S400:The ratio of the mold colony area and material sample block area is bigger,
The fungicidal properties of material sample block is poorer.
2. a kind of evaluation method of material sample block fungicidal properties according to claim 1, it is characterised in that the step
S200 also includes following methods：Camera lens and etalon are kept into distance to a declared goal A, etalon is taken pictures, standard is determined
The proportionate relationship of the unit sizes respective number of pixels in measurer unit sizes and photo；Camera lens and material sample block are kept at a distance A,
Material sample block is taken pictures, the mold colony on the block of material sample described in photo is demarcated, is determined mould on material sample block
The scope of bacterium bacterium colony；Pixel is counted in the mold colony area fixed to photo subscript, and face is included to material sample block in photo
Product respective number of pixels is counted；The real area and material of mold colony are calculated according to count results and the proportionate relationship
The real area of sample block.
3. the evaluation method of a kind of material sample block fungicidal properties according to claim 2, it is characterised in that also including following
Method：The scope of the mold colony is manually drawn a circle to approve according to the form of mold colony on material sample block.
4. the evaluation method of a kind of material sample block fungicidal properties according to claim 3, it is characterised in that also including following
Method：One square millimeter of the photo comprises at least 100 pixels.
5. the evaluation method of a kind of material sample block fungicidal properties according to claim 4, it is characterised in that also including following
Method：Mould is cultivated on material sample block using unified standard.
6. the evaluation method of a kind of material sample block fungicidal properties according to claim 5, it is characterised in that taken the photograph by regulation
The corresponding number of pixels of unit area is got on the corresponding number of pixels of unit area on camera regulation material sample block, material sample block
Many, the result of calculation of mold colony area is more accurate on material sample block.
7. a kind of evaluation method of material sample block fungicidal properties according to claim 2, it is characterised in that recording step
Lens focus and the proportionate relationship when camera lens is taken pictures to etalon in S200, make when being taken pictures to another material sample block
Material sample block and camera lens are kept at a distance A, lens focus when being taken pictures using camera lens in S200 to etalon；To institute in the photo
The mold colony stated on material sample block is demarcated, and pixel in the mold colony area demarcated on the photo is counted, right
Material sample block is counted comprising area respective number of pixels in the photo；This is calculated according to count results and the proportionate relationship
The real area of mold colony and the real area of material sample block.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107843595A (en) * | 2017-10-24 | 2018-03-27 | 南京苏试广博环境可靠性实验室有限公司 | Mould test method |
| CN111323207A (en) * | 2019-09-29 | 2020-06-23 | 云南北方光电仪器有限公司 | Method for evaluating mildew-proof and fog-proof capability of optical element |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107843595A (en) * | 2017-10-24 | 2018-03-27 | 南京苏试广博环境可靠性实验室有限公司 | Mould test method |
| CN107843595B (en) * | 2017-10-24 | 2020-06-26 | 南京苏试广博环境可靠性实验室有限公司 | Mould test method |
| CN111323207A (en) * | 2019-09-29 | 2020-06-23 | 云南北方光电仪器有限公司 | Method for evaluating mildew-proof and fog-proof capability of optical element |
| CN111323207B (en) * | 2019-09-29 | 2022-04-12 | 云南北方光电仪器有限公司 | Method for evaluating mildew-proof and fog-proof capability of optical element |
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Application publication date: 20171017 |
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